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Contents:

PCR primers for off-targets of TGGAGGGGAAGACGGCCCGG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529557_F TCGTCGGCAGCGTCCTCCCCTACCTGGTGCCT 59.9 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529557_R GTCTCGTGGGCTCGGTGGATGATGATATCGCCGCG 60.4 1.00
mm0__chr5 131.66 Mbp_chr5_131660016_F TCGTCGGCAGCGTCATGCCCATCATCACACCCTG 60.1 1.00
mm0__chr5 131.66 Mbp_chr5_131660016_R GTCTCGTGGGCTCGGTGTGGAAGGTCTGCTTCACG 60.2 1.00
mm1__chr2 73.90 Mbp_chr2_73901391_F TCGTCGGCAGCGTCAGGCTTCGCAGGAGATGATG 59.8 0.75
mm1__chr2 73.90 Mbp_chr2_73901391_R GTCTCGTGGGCTCGGTATCCAGATGAGCACGCACC 59.8 0.75
mm4__chr20 63.31 Mbp_chr20_63305079_F TCGTCGGCAGCGTCATAGGGTAGGAGCCTGTGGG 60.1 0.74
mm4__chr20 63.31 Mbp_chr20_63305079_R GTCTCGTGGGCTCGGGTATGAAGACAGGGCTGCCC 60.4 0.74
mm1__chr11 1.80 Mbp_chr11_1803995_F TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC 60.4 0.71
mm1__chr11 1.80 Mbp_chr11_1803995_R GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA 60.4 0.71
mm1__chr19 9.52 Mbp_chr19_9518226_F TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA 59.9 0.70
mm1__chr19 9.52 Mbp_chr19_9518226_R GTCTCGTGGGCTCGGGGAGTCCTTCTGACCGATGC 60.1 0.70
mm2__chr22 15.72 Mbp_chr22_15721563_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr22 15.72 Mbp_chr22_15721563_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.65
mm2__chr2 131.26 Mbp_chr2_131263632_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr2 131.26 Mbp_chr2_131263632_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.65
mm2__chr2 130.66 Mbp_chr2_130656826_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr2 130.66 Mbp_chr2_130656826_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.65
mm2__chr2 130.46 Mbp_chr2_130463843_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.65
mm2__chr2 130.46 Mbp_chr2_130463843_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr2 130.08 Mbp_chr2_130075271_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.65
mm2__chr2 130.08 Mbp_chr2_130075271_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr14 19.40 Mbp_chr14_19402808_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.65
mm2__chr14 19.40 Mbp_chr14_19402808_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr14 19.00 Mbp_chr14_18998944_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.65
mm2__chr14 19.00 Mbp_chr14_18998944_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.65
mm4__chr19 50.23 Mbp_chr19_50231971_F TCGTCGGCAGCGTCGACCCCACTCATTGTCCCTG 60.0 0.59
mm4__chr19 50.23 Mbp_chr19_50231971_R GTCTCGTGGGCTCGGCAGCGTCTCCTGGAAGAGTT 59.3 0.59
mm3__chr5 98.34 Mbp_chr5_98338819_F TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT 60.0 0.50
mm3__chr5 98.34 Mbp_chr5_98338819_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.50
mm4__chr3 159.13 Mbp_chr3_159133946_F Primer3: not found at this Tm N.d. 0.48
mm4__chr3 159.13 Mbp_chr3_159133946_R Primer3: not found at this Tm N.d. 0.48
mm4__chr2 60.49 Mbp_chr2_60489712_F Primer3: not found at this Tm N.d. 0.48
mm4__chr2 60.49 Mbp_chr2_60489712_R Primer3: not found at this Tm N.d. 0.48
mm4__chr15 34.53 Mbp_chr15_34528430_F TCGTCGGCAGCGTCTGGCATCTCTCTCTCCGGTA 59.4 0.48
mm4__chr15 34.53 Mbp_chr15_34528430_R GTCTCGTGGGCTCGGCTGCTGAGATGTGACCCCAT 59.4 0.48
mm4__chr15 34.38 Mbp_chr15_34382209_F TCGTCGGCAGCGTCTGGCATCTCTCTCTCCGGTA 59.4 0.48
mm4__chr15 34.38 Mbp_chr15_34382209_R GTCTCGTGGGCTCGGCTGCTGAGATGTGACCCCAT 59.4 0.48
mm4__chr15 101.77 Mbp_chr15_101772440_F TCGTCGGCAGCGTCCGCTCACTGATGGCTTCTGT 60.3 0.48
mm4__chr15 101.77 Mbp_chr15_101772440_R GTCTCGTGGGCTCGGGCTCCAGAGTGGCCTTATGG 60.1 0.48
mm4__chr1 52.55 Mbp_chr1_52549432_F Primer3: not found at this Tm N.d. 0.45
mm4__chr1 52.55 Mbp_chr1_52549432_R Primer3: not found at this Tm N.d. 0.45
mm4__chr1 112.79 Mbp_chr1_112794886_F Primer3: not found at this Tm N.d. 0.43
mm4__chr1 112.79 Mbp_chr1_112794886_R Primer3: not found at this Tm N.d. 0.43
mm3__chr22 41.08 Mbp_chr22_41075139_F TCGTCGGCAGCGTCGGAGTCCTTCTGACCCATGC 60.1 0.43
mm3__chr22 41.08 Mbp_chr22_41075139_R GTCTCGTGGGCTCGGTGTTGACAACAGCTCTGGCA 60.1 0.43
mm3__chr1 223.86 Mbp_chr1_223864751_F TCGTCGGCAGCGTCTGACCCATGCTCACCATCAC 60.0 0.43
mm3__chr1 223.86 Mbp_chr1_223864751_R GTCTCGTGGGCTCGGCGCTCGTCATTGACAACAGC 60.1 0.43
mm3__chr5 80.30 Mbp_chr5_80302747_F TCGTCGGCAGCGTCCTCTTGCTCTGGGCCTCATC 60.1 0.40
mm3__chr5 80.30 Mbp_chr5_80302747_R GTCTCGTGGGCTCGGTGGCATATGCAAGGCTAGCT 59.5 0.40
mm4__chr9 137.40 Mbp_chr9_137403842_F TCGTCGGCAGCGTCCTGGGCTCTTCACCAGGAAA 59.5 0.37
mm4__chr9 137.40 Mbp_chr9_137403842_R GTCTCGTGGGCTCGGGGGGTCTATGCAGAGATGGC 59.9 0.37
mm4__chr22 46.55 Mbp_chr22_46550559_F TCGTCGGCAGCGTCGAAAGGACTGGAGGTGAGGC 60.0 0.36
mm4__chr22 46.55 Mbp_chr22_46550559_R GTCTCGTGGGCTCGGTCTGGAATCCCTCCCTCCTG 60.0 0.36
mm4__chr3 194.61 Mbp_chr3_194608949_F Primer3: not found at this Tm N.d. 0.35
mm4__chr3 194.61 Mbp_chr3_194608949_R Primer3: not found at this Tm N.d. 0.35
mm4__chr2 131.63 Mbp_chr2_131626215_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.35
mm4__chr2 131.63 Mbp_chr2_131626215_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.35
mm4__chr22 22.72 Mbp_chr22_22721287_F TCGTCGGCAGCGTCTCATCCTCTCTCTCCAGCCC 60.1 0.34
mm4__chr22 22.72 Mbp_chr22_22721287_R GTCTCGTGGGCTCGGCACCTTCTGCAGAGCCTACC 60.1 0.34
mm4__chr5 80.98 Mbp_chr5_80980842_F Primer3: not found at this Tm N.d. 0.33
mm4__chr5 80.98 Mbp_chr5_80980842_R Primer3: not found at this Tm N.d. 0.33
mm4__chr3 32.24 Mbp_chr3_32238462_F TCGTCGGCAGCGTCTGACAGAAGTAAAGGCCGGG 59.6 0.32
mm4__chr3 32.24 Mbp_chr3_32238462_R GTCTCGTGGGCTCGGCCAGGGTGGCTGGGTTGG 62.6 0.32
mm4__chr7 43.13 Mbp_chr7_43131947_F TCGTCGGCAGCGTCTGGCACAAGTGTTCACCCTT 60.0 0.32
mm4__chr7 43.13 Mbp_chr7_43131947_R GTCTCGTGGGCTCGGTCGATGCCCTTGTAACCCAG 59.7 0.32
mm2__chr5 57.48 Mbp_chr5_57482604_F TCGTCGGCAGCGTCATCTCCCACGTAGCAGTCCT 60.0 0.32
mm2__chr5 57.48 Mbp_chr5_57482604_R GTCTCGTGGGCTCGGATGGGTCAGGGATGTGCAAG 60.0 0.32
mm4__chr18 79.54 Mbp_chr18_79544407_F TCGTCGGCAGCGTCCAGCTGGACCAAGGACAGG 60.0 0.32
mm4__chr18 79.54 Mbp_chr18_79544407_R GTCTCGTGGGCTCGGAGAGAGAGGGTGAGGAGCAG 60.0 0.32
mm4__chr2 239.39 Mbp_chr2_239392591_F TCGTCGGCAGCGTCCAAGCAAGGAGGCACAGTTG 59.6 0.31
mm4__chr2 239.39 Mbp_chr2_239392591_R GTCTCGTGGGCTCGGGTTCTAAGGAGGCTGACCCC 59.4 0.31
mm4__chr17 45.83 Mbp_chr17_45830610_F TCGTCGGCAGCGTCGTTCATCTGCATTGGCTGGG 59.5 0.30
mm4__chr17 45.83 Mbp_chr17_45830610_R GTCTCGTGGGCTCGGAACCCGACCTCCATCCCTC 60.6 0.30
mm4__chr19 11.02 Mbp_chr19_11022228_F TCGTCGGCAGCGTCGCGTCTCTGACCCTATGCTC 59.9 0.29
mm4__chr19 11.02 Mbp_chr19_11022228_R GTCTCGTGGGCTCGGCCTCTAGCTGTGCTCTCCAC 59.5 0.29
mm4__chr18 79.42 Mbp_chr18_79423790_F Primer3: not found at this Tm N.d. 0.27
mm4__chr18 79.42 Mbp_chr18_79423790_R Primer3: not found at this Tm N.d. 0.27
mm4__chr17 18.34 Mbp_chr17_18338414_F Primer3: not found at this Tm N.d. 0.27
mm4__chr17 18.34 Mbp_chr17_18338414_R Primer3: not found at this Tm N.d. 0.27
mm4__chr13 29.17 Mbp_chr13_29165231_F Primer3: not found at this Tm N.d. 0.25
mm4__chr13 29.17 Mbp_chr13_29165231_R Primer3: not found at this Tm N.d. 0.25
mm4__chr5 93.84 Mbp_chr5_93842969_F Primer3: not found at this Tm N.d. 0.24
mm4__chr5 93.84 Mbp_chr5_93842969_R Primer3: not found at this Tm N.d. 0.24
mm4__chr8 119.21 Mbp_chr8_119208666_F Primer3: not found at this Tm N.d. 0.24
mm4__chr8 119.21 Mbp_chr8_119208666_R Primer3: not found at this Tm N.d. 0.24
mm4__chr1 22.01 Mbp_chr1_22009860_F TCGTCGGCAGCGTCCTCGAGTCTCCGCCTTCATC 59.9 0.24
mm4__chr1 22.01 Mbp_chr1_22009860_R GTCTCGTGGGCTCGGAAGAGCCACCACCCTAAAGC 59.9 0.24
mm4__chr1 21.99 Mbp_chr1_21986686_F TCGTCGGCAGCGTCGGTGTTCACTCGAGTCTCCG 60.1 0.24
mm4__chr1 21.99 Mbp_chr1_21986686_R GTCTCGTGGGCTCGGAAGAGCCACCACCCTAAAGC 59.9 0.24
mm4__chr4 145.18 Mbp_chr4_145179760_F TCGTCGGCAGCGTCCTGCCTCCCCACCCAGAC 61.7 0.24
mm4__chr4 145.18 Mbp_chr4_145179760_R GTCTCGTGGGCTCGGGTATCGGAAACTGGTCGCCA 60.1 0.24
mm4__chr16 71.54 Mbp_chr16_71537294_F TCGTCGGCAGCGTCAAAGACCCCTCCCTCAACCT 60.1 0.24
mm4__chr16 71.54 Mbp_chr16_71537294_R GTCTCGTGGGCTCGGTCATGCTGCCCCATCACAAT 60.0 0.24
mm4__chr22 27.27 Mbp_chr22_27270611_F Primer3: not found at this Tm N.d. 0.24
mm4__chr22 27.27 Mbp_chr22_27270611_R Primer3: not found at this Tm N.d. 0.24
mm4__chr8 120.83 Mbp_chr8_120834114_F Primer3: not found at this Tm N.d. 0.23
mm4__chr8 120.83 Mbp_chr8_120834114_R Primer3: not found at this Tm N.d. 0.23
mm4__chr10 129.19 Mbp_chr10_129192599_F TCGTCGGCAGCGTCTGGTGACAGCACAGCAGTAG 59.9 0.23
mm4__chr10 129.19 Mbp_chr10_129192599_R GTCTCGTGGGCTCGGTGGCAAGATCCAATCTGCCC 60.3 0.23
mm4__chr3 129.92 Mbp_chr3_129918401_F Primer3: not found at this Tm N.d. 0.22
mm4__chr3 129.92 Mbp_chr3_129918401_R Primer3: not found at this Tm N.d. 0.22
mm4__chr8 84.95 Mbp_chr8_84948665_F TCGTCGGCAGCGTCCTCTGGTATGTGCAAGGCCA 60.0 0.21
mm4__chr8 84.95 Mbp_chr8_84948665_R GTCTCGTGGGCTCGGCAGTCCTTCTGCCCTATGCC 60.1 0.21
mm4__chr1 229.43 Mbp_chr1_229433009_F TCGTCGGCAGCGTCGCAGCCTGACCTGGTGTC 60.3 0.21
mm4__chr1 229.43 Mbp_chr1_229433009_R GTCTCGTGGGCTCGGGACGAAGACGAGACCACCG 60.1 0.21
mm4__chr1 147.59 Mbp_chr1_147591707_F TCGTCGGCAGCGTCTTCAGTCTCTGGCTTGGCTC 59.6 0.20
mm4__chr1 147.59 Mbp_chr1_147591707_R GTCTCGTGGGCTCGGAGGATCAGAAGCCAAACTGG 57.4 0.20
mm4__chr2 46.66 Mbp_chr2_46656422_F TCGTCGGCAGCGTCTATGTCACCACCCCTGCCTA 59.9 0.20
mm4__chr2 46.66 Mbp_chr2_46656422_R GTCTCGTGGGCTCGGTCTGGTGGGGATCAGTCCTT 59.8 0.20
mm4__chrX 103.38 Mbp_chrX_103377541_F TCGTCGGCAGCGTCAACGAAGAGATGGAGCAGCC 60.1 0.20
mm4__chrX 103.38 Mbp_chrX_103377541_R GTCTCGTGGGCTCGGCTGTCCCCGTCGATTTCCTG 60.4 0.20
mm4__chr7 50.40 Mbp_chr7_50399803_F TCGTCGGCAGCGTCCGTCAGTATGGTGCAGGTGT 60.0 0.19
mm4__chr7 50.40 Mbp_chr7_50399803_R GTCTCGTGGGCTCGGAGCTGAAATCTACAACCGGGG 60.0 0.19
mm4__chr3 38.01 Mbp_chr3_38009732_F TCGTCGGCAGCGTCGCCTCATCCTCCATCTCAGC 59.9 0.18
mm4__chr3 38.01 Mbp_chr3_38009732_R GTCTCGTGGGCTCGGCCACCTAGCAACTGAAGGGG 60.0 0.18
mm4__chr9 114.58 Mbp_chr9_114576054_F Primer3: not found at this Tm N.d. 0.18
mm4__chr9 114.58 Mbp_chr9_114576054_R Primer3: not found at this Tm N.d. 0.18
mm4__chr19 17.51 Mbp_chr19_17510733_F Primer3: not found at this Tm N.d. 0.17
mm4__chr19 17.51 Mbp_chr19_17510733_R Primer3: not found at this Tm N.d. 0.17
mm4__chr5 60.21 Mbp_chr5_60210813_F TCGTCGGCAGCGTCCCAGGTAGTTCTGGTAGGCT 58.1 0.17
mm4__chr5 60.21 Mbp_chr5_60210813_R GTCTCGTGGGCTCGGTGGCCTGCCATAAAAGAGCA 59.9 0.17
mm4__chrX 48.56 Mbp_chrX_48558825_F TCGTCGGCAGCGTCTTGTTTGTTCCCCTGCAGGT 60.0 0.17
mm4__chrX 48.56 Mbp_chrX_48558825_R GTCTCGTGGGCTCGGCTTCTAGCAATGGGCCTGGG 60.4 0.17
mm4__chr1 206.38 Mbp_chr1_206381341_F TCGTCGGCAGCGTCGGGTAAGCAACAGGGTAGAGG 59.7 0.17
mm4__chr1 206.38 Mbp_chr1_206381341_R GTCTCGTGGGCTCGGGAGTATCACTTCCCACCGCC 60.1 0.17
mm4__chr1 144.92 Mbp_chr1_144917402_F TCGTCGGCAGCGTCGAGTATCACTTCCCACCGCC 60.1 0.17
mm4__chr1 144.92 Mbp_chr1_144917402_R GTCTCGTGGGCTCGGGGGTAAGCAACAGGGTAGAGG 59.7 0.17
mm4__chr11 105.74 Mbp_chr11_105744057_F Primer3: not found at this Tm N.d. 0.17
mm4__chr11 105.74 Mbp_chr11_105744057_R Primer3: not found at this Tm N.d. 0.17
mm4__chr2 10.18 Mbp_chr2_10183463_F Primer3: not found at this Tm N.d. 0.16
mm4__chr2 10.18 Mbp_chr2_10183463_R Primer3: not found at this Tm N.d. 0.16
mm4__chr1 204.13 Mbp_chr1_204131754_F TCGTCGGCAGCGTCTGTGATGCTGGTTTGGAGCT 59.8 0.16
mm4__chr1 204.13 Mbp_chr1_204131754_R GTCTCGTGGGCTCGGTGTGTTTACCCCTTCCTGGC 59.8 0.16
mm4__chr1 117.84 Mbp_chr1_117843030_F Primer3: not found at this Tm N.d. 0.16
mm4__chr1 117.84 Mbp_chr1_117843030_R Primer3: not found at this Tm N.d. 0.16
mm4__chr20 61.66 Mbp_chr20_61658196_F TCGTCGGCAGCGTCTGCTCTCGCTTCCAGATCTC 59.2 0.15
mm4__chr20 61.66 Mbp_chr20_61658196_R GTCTCGTGGGCTCGGGTGCGATGGAGCCCTCTTTA 59.8 0.15
mm4__chr12 124.50 Mbp_chr12_124501910_F Primer3: not found at this Tm N.d. 0.15
mm4__chr12 124.50 Mbp_chr12_124501910_R Primer3: not found at this Tm N.d. 0.15
mm4__chr12 90.07 Mbp_chr12_90070769_F TCGTCGGCAGCGTCTCTTAGCCTCAGGGAAAGCC 59.0 0.15
mm4__chr12 90.07 Mbp_chr12_90070769_R GTCTCGTGGGCTCGGCGGTGTCTTACATCCCAGCA 59.7 0.15
mm4__chr22 43.74 Mbp_chr22_43739981_F Primer3: not found at this Tm N.d. 0.15
mm4__chr22 43.74 Mbp_chr22_43739981_R Primer3: not found at this Tm N.d. 0.15
mm3__chr14 99.25 Mbp_chr14_99254717_F TCGTCGGCAGCGTCTCATGGAGGGATTGGAGGACT 59.9 0.15
mm3__chr14 99.25 Mbp_chr14_99254717_R GTCTCGTGGGCTCGGAGGAATCACGGGCACTTCTC 59.7 0.15
mm4__chr12 130.43 Mbp_chr12_130425332_F TCGTCGGCAGCGTCACAGTTCACCAGACACGGAG 59.6 0.14
mm4__chr12 130.43 Mbp_chr12_130425332_R GTCTCGTGGGCTCGGGGAGAAGGGTGACACGTCAG 60.0 0.14
mm3__chr11 111.22 Mbp_chr11_111221709_F TCGTCGGCAGCGTCGCCAGGGGCTCTTAAGTATGT 59.5 0.14
mm3__chr11 111.22 Mbp_chr11_111221709_R GTCTCGTGGGCTCGGCTCTCTGCTTTCATGGGTGC 58.9 0.14
mm4__chr6 165.79 Mbp_chr6_165789069_F TCGTCGGCAGCGTCCTGCTCTTCTGGCTGAGTGG 60.3 0.14
mm4__chr6 165.79 Mbp_chr6_165789069_R GTCTCGTGGGCTCGGCAACCCTGAACTCCAGACCC 59.9 0.14
mm3__chr3 12.07 Mbp_chr3_12070301_F TCGTCGGCAGCGTCTCATTGACAGTGTCTCCGGC 60.0 0.14
mm3__chr3 12.07 Mbp_chr3_12070301_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.14
mm4__chr4 157.64 Mbp_chr4_157637765_F Primer3: not found at this Tm N.d. 0.14
mm4__chr4 157.64 Mbp_chr4_157637765_R Primer3: not found at this Tm N.d. 0.14
mm4__chr10 69.02 Mbp_chr10_69022830_F Primer3: not found at this Tm N.d. 0.14
mm4__chr10 69.02 Mbp_chr10_69022830_R Primer3: not found at this Tm N.d. 0.14
mm4__chr15 63.47 Mbp_chr15_63468580_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15 63.47 Mbp_chr15_63468580_R Primer3: not found at this Tm N.d. 0.13
mm4__chr10 117.38 Mbp_chr10_117375718_F TCGTCGGCAGCGTCGTGGCCGTCACTTCTGGAG 60.3 0.13
mm4__chr10 117.38 Mbp_chr10_117375718_R GTCTCGTGGGCTCGGGAGGATAACGGGATGGCCG 60.0 0.13
mm4__chr16 4.68 Mbp_chr16_4681608_F TCGTCGGCAGCGTCACAGAACTCTGACAGCGTCC 59.6 0.13
mm4__chr16 4.68 Mbp_chr16_4681608_R GTCTCGTGGGCTCGGCCGTCCGAGATGCCTGAATA 59.3 0.13
mm4__chr17 81.25 Mbp_chr17_81253108_F Primer3: not found at this Tm N.d. 0.13
mm4__chr17 81.25 Mbp_chr17_81253108_R Primer3: not found at this Tm N.d. 0.13
mm4__chr8 125.48 Mbp_chr8_125475321_F TCGTCGGCAGCGTCTGGAGTAAGGCCAGAGGTCA 59.8 0.13
mm4__chr8 125.48 Mbp_chr8_125475321_R GTCTCGTGGGCTCGGAGTCAGTAACGGTGTGCAGG 59.9 0.13
mm3__chr9 126.03 Mbp_chr9_126028190_F TCGTCGGCAGCGTCACAGAGCTCTCATCTCCCGA 59.7 0.13
mm3__chr9 126.03 Mbp_chr9_126028190_R GTCTCGTGGGCTCGGGCTGAGCTCAGTGTAGGGTG 60.1 0.13
mm4__chr20 62.65 Mbp_chr20_62653016_F Primer3: not found at this Tm N.d. 0.13
mm4__chr20 62.65 Mbp_chr20_62653016_R Primer3: not found at this Tm N.d. 0.13
mm4__chr8 120.20 Mbp_chr8_120196404_F Primer3: not found at this Tm N.d. 0.13
mm4__chr8 120.20 Mbp_chr8_120196404_R Primer3: not found at this Tm N.d. 0.13
mm4__chr1 50.15 Mbp_chr1_50152174_F TCGTCGGCAGCGTCCCCAGGATGTCTGTAGTCAGG 59.2 0.13
mm4__chr1 50.15 Mbp_chr1_50152174_R GTCTCGTGGGCTCGGGGAAGCAGGGCAGAACTGTC 60.9 0.13
mm4__chr22 21.55 Mbp_chr22_21548576_F TCGTCGGCAGCGTCCTGTCAGGCTCCAGGTCATC 59.8 0.13
mm4__chr22 21.55 Mbp_chr22_21548576_R GTCTCGTGGGCTCGGGCTCCCAGTCAAACTCCTCC 60.0 0.13
mm4__chr22 21.39 Mbp_chr22_21386235_F Primer3: not found at this Tm N.d. 0.13
mm4__chr22 21.39 Mbp_chr22_21386235_R Primer3: not found at this Tm N.d. 0.13
mm4__chr22 18.61 Mbp_chr22_18609034_F TCGTCGGCAGCGTCCTGTCAGGCTCCAGGTCATC 59.8 0.13
mm4__chr22 18.61 Mbp_chr22_18609034_R GTCTCGTGGGCTCGGGCTCCCAGTCAAACTCCTCC 60.0 0.13
mm4__chr21 22.91 Mbp_chr21_22907514_F Primer3: not found at this Tm N.d. 0.12
mm4__chr21 22.91 Mbp_chr21_22907514_R Primer3: not found at this Tm N.d. 0.12
mm4__chr7 155.79 Mbp_chr7_155788778_F TCGTCGGCAGCGTCCTTGGACAATGAGCAACCGC 60.1 0.12
mm4__chr7 155.79 Mbp_chr7_155788778_R GTCTCGTGGGCTCGGCCTTTCCCTGGCTGTGACTC 60.3 0.12
mm4__chr19 14.57 Mbp_chr19_14565698_F TCGTCGGCAGCGTCTGGATCGGTTTCGCCATCAT 59.8 0.12
mm4__chr19 14.57 Mbp_chr19_14565698_R GTCTCGTGGGCTCGGGGGCCACTGCAGGAAAGAA 60.2 0.12
mm4__chr6 31.14 Mbp_chr6_31142556_F TCGTCGGCAGCGTCCACCCACTTCTCCAGGATCC 59.4 0.12
mm4__chr6 31.14 Mbp_chr6_31142556_R GTCTCGTGGGCTCGGACCTTGACAGATGCTCCAGC 60.0 0.12
mm3__chr6 46.21 Mbp_chr6_46205052_F TCGTCGGCAGCGTCCGGCATGTGAAAAGCAGCTT 60.0 0.12
mm3__chr6 46.21 Mbp_chr6_46205052_R GTCTCGTGGGCTCGGCACGTAGGAGTCCTTCTGGC 59.8 0.12
mm4__chr9 127.49 Mbp_chr9_127492143_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 127.49 Mbp_chr9_127492143_R Primer3: not found at this Tm N.d. 0.12
mm4__chr2 18.96 Mbp_chr2_18961053_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 18.96 Mbp_chr2_18961053_R Primer3: not found at this Tm N.d. 0.12
mm4__chr8 12.95 Mbp_chr8_12951418_F TCGTCGGCAGCGTCGTCGTCGTCACCCAAGGTAC 60.3 0.11
mm4__chr8 12.95 Mbp_chr8_12951418_R GTCTCGTGGGCTCGGCCTTGTTGCCTGCCTCTCTC 60.6 0.11
mm3__chr9 2.02 Mbp_chr9_2016820_F TCGTCGGCAGCGTCCCCAACCTGGTTTACCCCTT 59.5 0.11
mm3__chr9 2.02 Mbp_chr9_2016820_R GTCTCGTGGGCTCGGTTTAGGGAAGTGCATCGCGA 59.7 0.11
mm4__chr21 8.45 Mbp_chr21_8445928_F Primer3: not found at this Tm N.d. 0.11
mm4__chr21 8.45 Mbp_chr21_8445928_R Primer3: not found at this Tm N.d. 0.11
mm4__chr21 8.22 Mbp_chr21_8218658_F Primer3: not found at this Tm N.d. 0.11
mm4__chr21 8.22 Mbp_chr21_8218658_R Primer3: not found at this Tm N.d. 0.11
mm4__chr3 42.96 Mbp_chr3_42957196_F Primer3: not found at this Tm N.d. 0.11
mm4__chr3 42.96 Mbp_chr3_42957196_R Primer3: not found at this Tm N.d. 0.11
mm4__chr19 44.82 Mbp_chr19_44820412_F TCGTCGGCAGCGTCCTAACCTTGTCTCCAGCCCC 59.7 0.11
mm4__chr19 44.82 Mbp_chr19_44820412_R GTCTCGTGGGCTCGGTAGCTGGAGTTGGCTTTGGG 59.9 0.11
mm4__chr7 5.44 Mbp_chr7_5439985_F TCGTCGGCAGCGTCCCACGGTCATCAGAGCCATC 60.5 0.11
mm4__chr7 5.44 Mbp_chr7_5439985_R GTCTCGTGGGCTCGGAGGTGCTGTTGACTCCTTGG 59.8 0.11
mm4__chr17 38.70 Mbp_chr17_38704095_F Primer3: not found at this Tm N.d. 0.10
mm4__chr17 38.70 Mbp_chr17_38704095_R Primer3: not found at this Tm N.d. 0.10
mm4__chr9 130.32 Mbp_chr9_130318968_F TCGTCGGCAGCGTCAGAAGGGGCTGTGGAAATGG 59.9 0.10
mm4__chr9 130.32 Mbp_chr9_130318968_R GTCTCGTGGGCTCGGTCTTGGAGGTGTCCTGAGCT 60.1 0.10
mm4__chrX 53.14 Mbp_chrX_53143812_F TCGTCGGCAGCGTCCATGGCCCATGTAGGAGTCC 59.8 0.10
mm4__chrX 53.14 Mbp_chrX_53143812_R GTCTCGTGGGCTCGGCTCTGGCATGTGCAAAGCTG 60.1 0.10
mm3__chr1_KI270714v1_random 40.26 Kbp_chr1_KI270714v1_random_40258_F Primer3: not found at this Tm N.d. 0.10
mm3__chr1_KI270714v1_random 40.26 Kbp_chr1_KI270714v1_random_40258_R Primer3: not found at this Tm N.d. 0.10
mm3__chr1 16.71 Mbp_chr1_16714347_F Primer3: not found at this Tm N.d. 0.10
mm3__chr1 16.71 Mbp_chr1_16714347_R Primer3: not found at this Tm N.d. 0.10
mm4__chr14 96.77 Mbp_chr14_96772334_F TCGTCGGCAGCGTCGGAAAGTATCAGGGGCCTGG 59.8 0.10
mm4__chr14 96.77 Mbp_chr14_96772334_R GTCTCGTGGGCTCGGTTTTCAGAAAGGCCCTGGCA 60.1 0.10
mm4__chr19 16.54 Mbp_chr19_16542976_F TCGTCGGCAGCGTCCGACGAGCTTGACTGGGATT 60.1 0.10
mm4__chr19 16.54 Mbp_chr19_16542976_R GTCTCGTGGGCTCGGCAGCAATTTGAGGGCATGGG 59.8 0.10
mm4__chr19 19.26 Mbp_chr19_19258450_F TCGTCGGCAGCGTCGGTCTCCTGTTTCTGATCGCT 59.7 0.10
mm4__chr19 19.26 Mbp_chr19_19258450_R GTCTCGTGGGCTCGGCTACGACGCCTTCTGCTTCA 60.1 0.10
mm4__chr19 38.51 Mbp_chr19_38512046_F TCGTCGGCAGCGTCTGGGGTGGATGTAGAGGGAG 60.0 0.10
mm4__chr19 38.51 Mbp_chr19_38512046_R GTCTCGTGGGCTCGGGGCCAGGATGTGAAGACAGT 59.6 0.10
mm3__chr12 49.30 Mbp_chr12_49295220_F TCGTCGGCAGCGTCCTAGCTCTGCGAACGGTGAC 60.7 0.10
mm3__chr12 49.30 Mbp_chr12_49295220_R GTCTCGTGGGCTCGGGAAGGTACGGCGGTATGAGG 59.9 0.10
mm4__chr7 100.66 Mbp_chr7_100655570_F TCGTCGGCAGCGTCTCCCTTTCTTCTCTTTGTCCCC 59.6 0.10
mm4__chr7 100.66 Mbp_chr7_100655570_R GTCTCGTGGGCTCGGTGACCTCTCTCCCTGCCAAT 60.2 0.10
mm4__chr15 26.89 Mbp_chr15_26891558_F TCGTCGGCAGCGTCTATGCGCGCTATTCAGCCAT 60.3 0.09
mm4__chr15 26.89 Mbp_chr15_26891558_R GTCTCGTGGGCTCGGCCCTAAACGTGCTTCGGTGA 60.3 0.09
mm4__chr6 158.82 Mbp_chr6_158819603_F TCGTCGGCAGCGTCAAGTGGGGAGGCTGTTTGTC 60.1 0.09
mm4__chr6 158.82 Mbp_chr6_158819603_R GTCTCGTGGGCTCGGCCAAGCGGTCCAGCGATT 60.4 0.09
mm4__chr3 50.47 Mbp_chr3_50473414_F TCGTCGGCAGCGTCCCCTCATGGCTCCAAGCTAC 60.1 0.09
mm4__chr3 50.47 Mbp_chr3_50473414_R GTCTCGTGGGCTCGGAAACAGCTGGGGTGATCTGG 59.9 0.09
mm4__chr2 109.45 Mbp_chr2_109446808_F TCGTCGGCAGCGTCTCTTTGGTGCCTGGGATGAG 59.6 0.09
mm4__chr2 109.45 Mbp_chr2_109446808_R GTCTCGTGGGCTCGGGACTGCACCAGGGTCTGTG 60.3 0.09
mm4__chr2 98.61 Mbp_chr2_98608630_F Primer3: not found at this Tm N.d. 0.09
mm4__chr2 98.61 Mbp_chr2_98608630_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 42.80 Mbp_chr17_42796800_F TCGTCGGCAGCGTCACTGAGCAAGGAAGATCCCA 58.3 0.09
mm4__chr17 42.80 Mbp_chr17_42796800_R GTCTCGTGGGCTCGGACACCTCCACACAGCAAACA 60.0 0.09
mm4__chr11 40.29 Mbp_chr11_40293454_F TCGTCGGCAGCGTCCAGATCCTCCAGGTGTTGCA 59.6 0.09
mm4__chr11 40.29 Mbp_chr11_40293454_R GTCTCGTGGGCTCGGCTCCCGGAGCCACTACCG 61.5 0.09
mm4__chr11 70.75 Mbp_chr11_70745446_F TCGTCGGCAGCGTCGGGCTCACAGTCCCTCAAAA 59.8 0.09
mm4__chr11 70.75 Mbp_chr11_70745446_R GTCTCGTGGGCTCGGGACAGAGCCAAGGGACACAG 60.3 0.09
mm2__chr5 138.25 Mbp_chr5_138252936_F TCGTCGGCAGCGTCCTGCTGCTGTCCTTTCCTCA 59.9 0.09
mm2__chr5 138.25 Mbp_chr5_138252936_R GTCTCGTGGGCTCGGTGCACGCTTCCCTTGATTCT 59.9 0.09
mm4__chr6 101.43 Mbp_chr6_101431437_F TCGTCGGCAGCGTCTGATGCTGTCCTGTGTGCTT 59.8 0.09
mm4__chr6 101.43 Mbp_chr6_101431437_R GTCTCGTGGGCTCGGCGGCTTTGCTGGGGATGA 60.0 0.09
mm4__chr19 19.32 Mbp_chr19_19321008_F TCGTCGGCAGCGTCCTGAGCACTTCCGCACTTCC 61.2 0.08
mm4__chr19 19.32 Mbp_chr19_19321008_R GTCTCGTGGGCTCGGGATAGAGAACGGAGCCCAGC 59.9 0.08
mm4__chr3 128.85 Mbp_chr3_128850660_F TCGTCGGCAGCGTCGTCTTGCCCAGTTCTCCCAT 59.6 0.08
mm4__chr3 128.85 Mbp_chr3_128850660_R GTCTCGTGGGCTCGGCCATGTCAGTCAAATGTCTTTCTGT 59.7 0.08
mm4__chr3 12.87 Mbp_chr3_12871737_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 12.87 Mbp_chr3_12871737_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 11.91 Mbp_chr3_11914212_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 11.91 Mbp_chr3_11914212_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 11.91 Mbp_chr3_11909090_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 11.91 Mbp_chr3_11909090_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 10.01 Mbp_chr3_10006808_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 10.01 Mbp_chr3_10006808_R Primer3: not found at this Tm N.d. 0.08
mm4__chr13 113.08 Mbp_chr13_113083520_F TCGTCGGCAGCGTCTGCTCATGCCCCAAATCCAT 60.0 0.08
mm4__chr13 113.08 Mbp_chr13_113083520_R GTCTCGTGGGCTCGGCCTGGGCTGTTTTCTGTTGC 59.9 0.08
mm4__chr7 153.94 Mbp_chr7_153935009_F TCGTCGGCAGCGTCAAAACAAGGCTTCGGCACTG 59.6 0.08
mm4__chr7 153.94 Mbp_chr7_153935009_R GTCTCGTGGGCTCGGTGTCAGGACATTCGCCATCC 60.1 0.08
mm4__chr14 31.95 Mbp_chr14_31951620_F TCGTCGGCAGCGTCCCCTCCAGTCCCCTCATAGA 59.4 0.08
mm4__chr14 31.95 Mbp_chr14_31951620_R GTCTCGTGGGCTCGGGTGGTAATGTTCAGGGAGCCT 59.7 0.08
mm4__chr1 109.48 Mbp_chr1_109484842_F TCGTCGGCAGCGTCCATCACGCCCCTGAAGAAGT 60.0 0.08
mm4__chr1 109.48 Mbp_chr1_109484842_R GTCTCGTGGGCTCGGCGAAACTGGGAGTCGGATGT 59.7 0.08
mm4__chr6 144.20 Mbp_chr6_144200851_F Primer3: not found at this Tm N.d. 0.08
mm4__chr6 144.20 Mbp_chr6_144200851_R Primer3: not found at this Tm N.d. 0.08
mm4__chr12 9.16 Mbp_chr12_9156321_F TCGTCGGCAGCGTCTCTGCTTCCAGTACCTTCGC 59.7 0.08
mm4__chr12 9.16 Mbp_chr12_9156321_R GTCTCGTGGGCTCGGGATGGAGGAAGGACTGTGCC 60.1 0.08
mm4__chr17 81.04 Mbp_chr17_81038556_F TCGTCGGCAGCGTCGCCCTGGCTGGTCACTCTAG 61.9 0.08
mm4__chr17 81.04 Mbp_chr17_81038556_R GTCTCGTGGGCTCGGTACCAATCACACCAGAGGCG 59.7 0.08
mm4__chr10 45.30 Mbp_chr10_45303669_F TCGTCGGCAGCGTCGCAGGTGGAGAAGGCTTTCT 59.9 0.07
mm4__chr10 45.30 Mbp_chr10_45303669_R GTCTCGTGGGCTCGGTCGCGTCCTATGGCTTCATC 59.9 0.07
mm4__chr9 93.58 Mbp_chr9_93576265_F Primer3: not found at this Tm N.d. 0.07
mm4__chr9 93.58 Mbp_chr9_93576265_R Primer3: not found at this Tm N.d. 0.07
mm4__chr12 132.32 Mbp_chr12_132321980_F Primer3: not found at this Tm N.d. 0.07
mm4__chr12 132.32 Mbp_chr12_132321980_R Primer3: not found at this Tm N.d. 0.07
mm4__chr2 19.80 Mbp_chr2_19799444_F TCGTCGGCAGCGTCGCCGAAGAGTCCAAAACAGC 59.7 0.07
mm4__chr2 19.80 Mbp_chr2_19799444_R GTCTCGTGGGCTCGGACTCCTCTCCCTTGTCCCTC 59.9 0.07
mm3__chr5 154.59 Mbp_chr5_154590930_F TCGTCGGCAGCGTCTCCCTGAAGCCTGGAAGGAT 60.2 0.06
mm3__chr5 154.59 Mbp_chr5_154590930_R GTCTCGTGGGCTCGGGCCAGGAAACCATTCGGAGA 60.0 0.06
mm3__chr2 239.03 Mbp_chr2_239026891_F Primer3: not found at this Tm N.d. 0.06
mm3__chr2 239.03 Mbp_chr2_239026891_R Primer3: not found at this Tm N.d. 0.06
mm4__chr16 894.08 Kbp_chr16_894083_F Primer3: not found at this Tm N.d. 0.06
mm4__chr16 894.08 Kbp_chr16_894083_R Primer3: not found at this Tm N.d. 0.06
mm4__chr9 1.05 Mbp_chr9_1048247_F TCGTCGGCAGCGTCGGAACAGAGGCTTACACCCC 60.0 0.05
mm4__chr9 1.05 Mbp_chr9_1048247_R GTCTCGTGGGCTCGGAGCCCTTCACCAGTTGTTTT 57.8 0.05
mm3__chrX 44.77 Mbp_chrX_44765430_F TCGTCGGCAGCGTCACCTCTTCTGACCCTGGTGT 60.1 0.05
mm3__chrX 44.77 Mbp_chrX_44765430_R GTCTCGTGGGCTCGGTCTGGGGAGAGGAAAGCCAA 60.4 0.05
mm4__chr12 131.85 Mbp_chr12_131846283_F TCGTCGGCAGCGTCCATCCAGTGTCACCCTCAGC 60.3 0.05
mm4__chr12 131.85 Mbp_chr12_131846283_R GTCTCGTGGGCTCGGCAAGGCCCGGCTTCCTTATA 59.5 0.05
mm4__chr13 99.89 Mbp_chr13_99894959_F TCGTCGGCAGCGTCCCGTCTGATGTCCCAGTGTT 59.6 0.05
mm4__chr13 99.89 Mbp_chr13_99894959_R GTCTCGTGGGCTCGGTTCAAATCGGAGGCGGTGAA 59.9 0.05
mm4__chr16 173.64 Kbp_chr16_173641_F TCGTCGGCAGCGTCCACCGTGCTGACCTCCAAAT 60.6 0.05
mm4__chr16 173.64 Kbp_chr16_173641_R GTCTCGTGGGCTCGGCTGCTGCCCACTCAGACTTT 60.2 0.05
mm4__chr10 132.11 Mbp_chr10_132108474_F TCGTCGGCAGCGTCATCTCCTCAGCTTCCAGGGT 59.9 0.05
mm4__chr10 132.11 Mbp_chr10_132108474_R GTCTCGTGGGCTCGGCTCTTGTCTGAGGCTCCACC 59.7 0.05
mm2__chr7 75.99 Mbp_chr7_75988755_F Primer3: not found at this Tm N.d. 0.05
mm2__chr7 75.99 Mbp_chr7_75988755_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 113.06 Mbp_chr1_113063531_F Primer3: not found at this Tm N.d. 0.05
mm4__chr1 113.06 Mbp_chr1_113063531_R Primer3: not found at this Tm N.d. 0.05
mm4__chr18 37.42 Mbp_chr18_37423402_F TCGTCGGCAGCGTCGGAAAGGAGTCCTTGTGGGG 59.9 0.04
mm4__chr18 37.42 Mbp_chr18_37423402_R GTCTCGTGGGCTCGGTGACTTCAAGGGTGCCTCAC 59.8 0.04
mm4__chr1 224.50 Mbp_chr1_224501967_F TCGTCGGCAGCGTCAGGAGGCTGGAGGAAGGTAG 60.0 0.04
mm4__chr1 224.50 Mbp_chr1_224501967_R GTCTCGTGGGCTCGGTCCCCACATTCTTCAGCACC 59.9 0.04
mm4__chr16 169.38 Kbp_chr16_169384_F TCGTCGGCAGCGTCTGTGAGTGCTGTGTTGACCT 59.4 0.04
mm4__chr16 169.38 Kbp_chr16_169384_R GTCTCGTGGGCTCGGAAGATCAGGAAGTGCTGGGG 59.3 0.04
mm4__chr14 104.68 Mbp_chr14_104681991_F TCGTCGGCAGCGTCGAGCTGTCACTCTCCCAAGG 59.7 0.04
mm4__chr14 104.68 Mbp_chr14_104681991_R GTCTCGTGGGCTCGGCTGCTGGCTCTTCTGGGAC 60.0 0.04
mm4__chr19 30.31 Mbp_chr19_30314985_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 30.31 Mbp_chr19_30314985_R Primer3: not found at this Tm N.d. 0.04
mm2__chr18 62.44 Mbp_chr18_62442085_F TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT 60.1 0.04
mm2__chr18 62.44 Mbp_chr18_62442085_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.04
mm1__chr6 88.28 Mbp_chr6_88276907_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.04
mm1__chr6 88.28 Mbp_chr6_88276907_R GTCTCGTGGGCTCGGTGGATGATGCTATCACCGCG 60.3 0.04
mm4__chr20 47.68 Mbp_chr20_47683342_F Primer3: not found at this Tm N.d. 0.04
mm4__chr20 47.68 Mbp_chr20_47683342_R Primer3: not found at this Tm N.d. 0.04
mm4__chrX 108.16 Mbp_chrX_108156298_F Primer3: not found at this Tm N.d. 0.04
mm4__chrX 108.16 Mbp_chrX_108156298_R Primer3: not found at this Tm N.d. 0.04
mm4__chrX 46.29 Mbp_chrX_46289029_F Primer3: not found at this Tm N.d. 0.04
mm4__chrX 46.29 Mbp_chrX_46289029_R Primer3: not found at this Tm N.d. 0.04
mm4__chr15 88.82 Mbp_chr15_88816098_F Primer3: not found at this Tm N.d. 0.04
mm4__chr15 88.82 Mbp_chr15_88816098_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 34.48 Mbp_chr14_34476256_F Primer3: not found at this Tm N.d. 0.04
mm4__chr14 34.48 Mbp_chr14_34476256_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 13.78 Mbp_chr1_13780694_F TCGTCGGCAGCGTCGCTGCCTCCTCTCTTGATCC 59.8 0.04
mm4__chr1 13.78 Mbp_chr1_13780694_R GTCTCGTGGGCTCGGATGGGTGTGGAGATGCACTG 60.0 0.04
mm4__chr1 226.38 Mbp_chr1_226380068_F TCGTCGGCAGCGTCGCCACAACTTCAACAGGCTC 59.6 0.04
mm4__chr1 226.38 Mbp_chr1_226380068_R GTCTCGTGGGCTCGGGCCAACATCCGAGTTGTGTC 59.4 0.04
mm4__chr4 45.03 Mbp_chr4_45034164_F TCGTCGGCAGCGTCGAGGACAAGGAGGGCCATTC 60.1 0.04
mm4__chr4 45.03 Mbp_chr4_45034164_R GTCTCGTGGGCTCGGGCAGACAGACTCCCCTAAGG 59.1 0.04
mm4__chr20 57.53 Mbp_chr20_57526423_F TCGTCGGCAGCGTCGGAATGAGGAGGGGAGGAGA 59.7 0.03
mm4__chr20 57.53 Mbp_chr20_57526423_R GTCTCGTGGGCTCGGGGCCATCCTCCCCTCCTTAT 60.4 0.03
mm3__chr6 42.78 Mbp_chr6_42782778_F TCGTCGGCAGCGTCGTGTGAGTGTGTTTTCCGCC 59.9 0.03
mm3__chr6 42.78 Mbp_chr6_42782778_R GTCTCGTGGGCTCGGTTTTCTTCCTCGCCCGCC 60.3 0.03
mm4__chr20 24.07 Mbp_chr20_24066266_F TCGTCGGCAGCGTCTCAGACAGGTGGAGGAGGAG 59.9 0.03
mm4__chr20 24.07 Mbp_chr20_24066266_R GTCTCGTGGGCTCGGAGCAAAGTCTGAGAGCCCAG 59.6 0.03
mm4__chr10 16.73 Mbp_chr10_16730112_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 16.73 Mbp_chr10_16730112_R Primer3: not found at this Tm N.d. 0.03
mm4__chr9 113.88 Mbp_chr9_113876826_F TCGTCGGCAGCGTCTGCAATTTTCCCCTGACCCT 59.5 0.03
mm4__chr9 113.88 Mbp_chr9_113876826_R GTCTCGTGGGCTCGGAAATTGAGAAAGGAAAATATGGCCT 57.4 0.03
mm4__chr15 69.39 Mbp_chr15_69393673_F TCGTCGGCAGCGTCGGAGAGAACCACCTTCCCCT 60.5 0.03
mm4__chr15 69.39 Mbp_chr15_69393673_R GTCTCGTGGGCTCGGTCAACCCTGATTTTCGGGCA 59.8 0.03
mm4__chr10 4.19 Mbp_chr10_4188630_F TCGTCGGCAGCGTCGACGGGGTTCATGGAGCTTA 59.4 0.03
mm4__chr10 4.19 Mbp_chr10_4188630_R GTCTCGTGGGCTCGGAGTCTGATTGCAACAGGCCA 59.8 0.03
mm4__chr20 24.79 Mbp_chr20_24787469_F TCGTCGGCAGCGTCTTCCTCCCCATACATTCGCC 59.5 0.03
mm4__chr20 24.79 Mbp_chr20_24787469_R GTCTCGTGGGCTCGGAGACATGGAGGGAGGGAGTC 60.0 0.03
mm3__chr7 155.35 Mbp_chr7_155350669_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350669_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350597_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350597_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350525_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350525_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350453_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350453_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350417_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350417_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 155.35 Mbp_chr7_155350237_F TCGTCGGCAGCGTCTCCCTCGGCTTCCTAGAGAC 60.1 0.03
mm3__chr7 155.35 Mbp_chr7_155350237_R GTCTCGTGGGCTCGGGAAGTCGGCCCGGGAGAC 62.1 0.03
mm4__chr10 132.10 Mbp_chr10_132103472_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 132.10 Mbp_chr10_132103472_R Primer3: not found at this Tm N.d. 0.03
mm2__chr3 175.98 Mbp_chr3_175978479_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.03
mm2__chr3 175.98 Mbp_chr3_175978479_R GTCTCGTGGGCTCGGGCAAAGGCTCCTGTATGTGC 59.5 0.03
mm4__chr12 48.89 Mbp_chr12_48890981_F TCGTCGGCAGCGTCGCGACTGAGGTCAGAGCC 59.8 0.03
mm4__chr12 48.89 Mbp_chr12_48890981_R GTCTCGTGGGCTCGGGCAGAAGCCACCACCTCTAA 59.6 0.03
mm4__chrX 150.69 Mbp_chrX_150693807_F TCGTCGGCAGCGTCGGCGGCCCCTCTTCATCC 62.5 0.03
mm4__chrX 150.69 Mbp_chrX_150693807_R GTCTCGTGGGCTCGGATGGGGCGCTGAGGTTTC 60.0 0.03
mm4__chr3 94.14 Mbp_chr3_94140001_F TCGTCGGCAGCGTCAGGTGTTATATGCCATGTCATTCA 58.3 0.03
mm4__chr3 94.14 Mbp_chr3_94140001_R GTCTCGTGGGCTCGGTTACAGGCCTACACGGGGTA 59.9 0.03
mm4__chr1 6.43 Mbp_chr1_6433699_F Primer3: not found at this Tm N.d. 0.03
mm4__chr1 6.43 Mbp_chr1_6433699_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 7.56 Mbp_chr1_7559386_F Primer3: not found at this Tm N.d. 0.03
mm4__chr1 7.56 Mbp_chr1_7559386_R Primer3: not found at this Tm N.d. 0.03
mm4__chr3 135.84 Mbp_chr3_135839632_F TCGTCGGCAGCGTCCCCAGCCTCCAAACCAATGA 60.2 0.03
mm4__chr3 135.84 Mbp_chr3_135839632_R GTCTCGTGGGCTCGGAGGGGTAGAGCAGCATCTCA 60.0 0.03
mm4__chr17 18.98 Mbp_chr17_18982581_F Primer3: not found at this Tm N.d. 0.02
mm4__chr17 18.98 Mbp_chr17_18982581_R Primer3: not found at this Tm N.d. 0.02
mm4__chr12 13.83 Mbp_chr12_13833369_F TCGTCGGCAGCGTCTGGCTTAGGACAGTCACAATGG 60.2 0.02
mm4__chr12 13.83 Mbp_chr12_13833369_R GTCTCGTGGGCTCGGGAATCAGGGAAGGGCTGGAG 59.8 0.02
mm4__chr11 65.61 Mbp_chr11_65607771_F Primer3: not found at this Tm N.d. 0.02
mm4__chr11 65.61 Mbp_chr11_65607771_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 63.91 Mbp_chr15_63905629_F TCGTCGGCAGCGTCGCCATCCAACACCAGAAAGC 59.7 0.02
mm4__chr15 63.91 Mbp_chr15_63905629_R GTCTCGTGGGCTCGGAACCAGGACTCCATCATGCC 59.7 0.02
mm4__chr3 195.76 Mbp_chr3_195763321_F TCGTCGGCAGCGTCATTTACTCCGGGACAGCTGC 60.1 0.02
mm4__chr3 195.76 Mbp_chr3_195763321_R GTCTCGTGGGCTCGGACATACTGAGGCCGAGGAGA 59.7 0.02
mm3__chr18 79.77 Mbp_chr18_79771490_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771490_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771473_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771473_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771439_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771439_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771422_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771422_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771354_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771354_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771320_F TCGTCGGCAGCGTCCATCCTCCCCTCCACGCC 61.8 0.02
mm3__chr18 79.77 Mbp_chr18_79771320_R GTCTCGTGGGCTCGGATGTCATGGAGGGGAGGCT 59.9 0.02
mm3__chr18 79.77 Mbp_chr18_79771303_F TCGTCGGCAGCGTCCATCCTCCCCTCCACGCC 61.8 0.02
mm3__chr18 79.77 Mbp_chr18_79771303_R GTCTCGTGGGCTCGGATGTCATGGAGGGGAGGCT 59.9 0.02
mm3__chr18 79.77 Mbp_chr18_79771269_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771269_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771235_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771235_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771150_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771150_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771133_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.02
mm3__chr18 79.77 Mbp_chr18_79771133_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.02
mm3__chr18 79.77 Mbp_chr18_79771116_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.02
mm3__chr18 79.77 Mbp_chr18_79771116_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.02
mm3__chr18 79.77 Mbp_chr18_79771099_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.02
mm3__chr18 79.77 Mbp_chr18_79771099_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.02
mm3__chr18 79.77 Mbp_chr18_79771065_F Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79771065_R Primer3: not found at this Tm N.d. 0.02
mm3__chr18 79.77 Mbp_chr18_79770997_F TCGTCGGCAGCGTCCTGGGGTCGGAATCCAAAGG 60.3 0.02
mm3__chr18 79.77 Mbp_chr18_79770997_R GTCTCGTGGGCTCGGCCTGTGTGGAGGGGAGGA 59.8 0.02
mm4__chr12 31.23 Mbp_chr12_31226362_F TCGTCGGCAGCGTCGAGGGAATGCACCTGCCTAG 60.1 0.02
mm4__chr12 31.23 Mbp_chr12_31226362_R GTCTCGTGGGCTCGGGGTCTTTCCAAGGACTCCGG 60.0 0.02
mm4__chr22 47.06 Mbp_chr22_47057335_F TCGTCGGCAGCGTCAGCAGAAGTTCTTGCCTCCC 59.9 0.02
mm4__chr22 47.06 Mbp_chr22_47057335_R GTCTCGTGGGCTCGGAGCACTGTCCATCTGGGTTC 59.6 0.02
mm4__chr22 46.25 Mbp_chr22_46250688_F TCGTCGGCAGCGTCGACTAGGCTGCCAGTCTACC 59.2 0.02
mm4__chr22 46.25 Mbp_chr22_46250688_R GTCTCGTGGGCTCGGCTTTGGAGGAGCAGGGACAG 60.0 0.02
mm4__chr13 73.06 Mbp_chr13_73059801_F TCGTCGGCAGCGTCCGTTTCGCTGAGAGTAAATGGG 59.6 0.01
mm4__chr13 73.06 Mbp_chr13_73059801_R GTCTCGTGGGCTCGGCCCTCCCTCTTACCTCTCCC 60.1 0.01
mm4__chr15 75.58 Mbp_chr15_75579440_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 75.58 Mbp_chr15_75579440_R Primer3: not found at this Tm N.d. 0.01
mm4__chr15 66.37 Mbp_chr15_66370475_F TCGTCGGCAGCGTCCGGACGTGAAGAACCAAGGA 59.9 0.01
mm4__chr15 66.37 Mbp_chr15_66370475_R GTCTCGTGGGCTCGGTACTATCCCCTCTGCACCCC 60.1 0.01
mm4__chr21 40.56 Mbp_chr21_40564940_F TCGTCGGCAGCGTCCTAGTTGCTCCCTGTGCCTC 60.1 0.01
mm4__chr21 40.56 Mbp_chr21_40564940_R GTCTCGTGGGCTCGGGAGCAGTATCCTCCCCTCCA 60.1 0.01
mm4__chr1 33.99 Mbp_chr1_33985149_F TCGTCGGCAGCGTCACAGCTTCAAGGCTTCACCA 59.8 0.01
mm4__chr1 33.99 Mbp_chr1_33985149_R GTCTCGTGGGCTCGGTGGATGGGGAAGGAAGGCTA 59.9 0.01
mm4__chr19 15.45 Mbp_chr19_15445454_F TCGTCGGCAGCGTCCTCCATCCAGAGAAGTGGCG 60.1 0.01
mm4__chr19 15.45 Mbp_chr19_15445454_R GTCTCGTGGGCTCGGCCCCTTGAGACATCGCTGAA 59.7 0.01
mm4__chr5 43.00 Mbp_chr5_43001171_F TCGTCGGCAGCGTCTCGCCTGGATTTGCTGGTT 59.9 0.01
mm4__chr5 43.00 Mbp_chr5_43001171_R GTCTCGTGGGCTCGGGCTTAGCCTGTGAGTCCCC 59.7 0.01
mm4__chr20 49.48 Mbp_chr20_49482197_F TCGTCGGCAGCGTCTCCTCCATCATGTGCAGTCG 59.8 0.01
mm4__chr20 49.48 Mbp_chr20_49482197_R GTCTCGTGGGCTCGGGTGTGCGATGACTACAGCCT 60.1 0.01
mm4__chr18 2.91 Mbp_chr18_2908702_F TCGTCGGCAGCGTCGCTCCAGTGCCCATCTCTTT 60.0 0.01
mm4__chr18 2.91 Mbp_chr18_2908702_R GTCTCGTGGGCTCGGCTCCTTCAAGCAGAGGCACA 59.9 0.01
mm4__chr18 79.77 Mbp_chr18_79771337_F TCGTCGGCAGCGTCCATCCTCCCCTCCACGCC 61.8 0.01
mm4__chr18 79.77 Mbp_chr18_79771337_R GTCTCGTGGGCTCGGATGTCATGGAGGGGAGGCT 59.9 0.01
mm4__chr18 79.77 Mbp_chr18_79771252_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 79.77 Mbp_chr18_79771252_R Primer3: not found at this Tm N.d. 0.01
mm4__chr18 79.77 Mbp_chr18_79771201_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 79.77 Mbp_chr18_79771201_R Primer3: not found at this Tm N.d. 0.01
mm3__chr14 104.32 Mbp_chr14_104319519_F Primer3: not found at this Tm N.d. 0.01
mm3__chr14 104.32 Mbp_chr14_104319519_R Primer3: not found at this Tm N.d. 0.01
mm4__chr14 74.41 Mbp_chr14_74407620_F TCGTCGGCAGCGTCCACCACAGCCACGTAGAGAC 60.3 0.01
mm4__chr14 74.41 Mbp_chr14_74407620_R GTCTCGTGGGCTCGGCCTAGACCAGCAAGGCCATC 60.1 0.01
mm4__chr1 99.00 Mbp_chr1_99004161_F TCGTCGGCAGCGTCCCCCAAAGCTCACCTCCTTT 59.8 0.01
mm4__chr1 99.00 Mbp_chr1_99004161_R GTCTCGTGGGCTCGGCAGCTCTTCCCACACGTCAC 60.9 0.01
mm4__chr2 10.28 Mbp_chr2_10277915_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 10.28 Mbp_chr2_10277915_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 4.06 Mbp_chr1_4058990_F TCGTCGGCAGCGTCGTCACCACCTTGCTCTGGTT 60.1 0.01
mm4__chr1 4.06 Mbp_chr1_4058990_R GTCTCGTGGGCTCGGTCTGCAAGGGACCAGCATTT 59.8 0.01
mm3__chr2 100.84 Mbp_chr2_100835853_F TCGTCGGCAGCGTCGAAGTCACTCAGGGTCACCC 59.6 0.01
mm3__chr2 100.84 Mbp_chr2_100835853_R GTCTCGTGGGCTCGGTTGAGTGCTGAGGTCTGTGC 60.2 0.01
mm4__chr8 37.97 Mbp_chr8_37965322_F Primer3: not found at this Tm N.d. 0.01
mm4__chr8 37.97 Mbp_chr8_37965322_R Primer3: not found at this Tm N.d. 0.01
mm4__chrY 280.75 Kbp_chrY_280752_F Primer3: not found at this Tm N.d. 0.01
mm4__chrY 280.75 Kbp_chrY_280752_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 16.42 Mbp_chr17_16417141_F TCGTCGGCAGCGTCCAACATCAAAAGGGTGGCCC 59.6 0.00
mm4__chr17 16.42 Mbp_chr17_16417141_R GTCTCGTGGGCTCGGGTAGGTCCACCCACTGTGTG 59.9 0.00
mm4__chr9 117.09 Mbp_chr9_117091659_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 117.09 Mbp_chr9_117091659_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.83 Mbp_chr1_10834502_F TCGTCGGCAGCGTCGCCAGTGAATCCAGGGAGAC 60.1 0.00
mm4__chr1 10.83 Mbp_chr1_10834502_R GTCTCGTGGGCTCGGTATACCATGCAGTGCCACCC 59.8 0.00
mm4__chr17 76.26 Mbp_chr17_76261917_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 76.26 Mbp_chr17_76261917_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 23.04 Mbp_chr20_23044019_F Primer3: not found at this Tm N.d. 0.00
mm4__chr20 23.04 Mbp_chr20_23044019_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 178.76 Mbp_chr1_178758533_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 178.76 Mbp_chr1_178758533_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 14.51 Mbp_chr19_14506107_F TCGTCGGCAGCGTCAACAAGCTCCTGTCCCTTGG 59.8 0.00
mm4__chr19 14.51 Mbp_chr19_14506107_R GTCTCGTGGGCTCGGGACCGAGCCACTTCCCATAG 59.8 0.00
mm4__chr18 79.77 Mbp_chr18_79771218_F Primer3: not found at this Tm N.d. 0.00
mm4__chr18 79.77 Mbp_chr18_79771218_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 79.77 Mbp_chr18_79771031_F TCGTCGGCAGCGTCCTGGGGTCGGAATCCAAAGG 60.3 0.00
mm4__chr18 79.77 Mbp_chr18_79771031_R GTCTCGTGGGCTCGGCTGGCATGGAGGGGAGGA 60.3 0.00
mm3__chr20 61.80 Mbp_chr20_61800027_F TCGTCGGCAGCGTCTCTCCGCTTGTCTTCATGGC 60.3 0.00
mm3__chr20 61.80 Mbp_chr20_61800027_R GTCTCGTGGGCTCGGGAACCCCTCAGAGGAAGCAC 60.0 0.00
mm4__chr7 29.42 Mbp_chr7_29415587_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 29.42 Mbp_chr7_29415587_R Primer3: not found at this Tm N.d. 0.00
mm3__chr19 3.64 Mbp_chr19_3643634_F Primer3: not found at this Tm N.d. 0.00
mm3__chr19 3.64 Mbp_chr19_3643634_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 79.77 Mbp_chr18_79771524_F TCGTCGGCAGCGTCTCCTCCCCTCCACACAGG 59.8 0.00
mm4__chr18 79.77 Mbp_chr18_79771524_R GTCTCGTGGGCTCGGCTGGGAAGCTGCAGGCAT 60.0 0.00
mm4__chr18 79.77 Mbp_chr18_79771456_F Primer3: not found at this Tm N.d. 0.00
mm4__chr18 79.77 Mbp_chr18_79771456_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 63.06 Mbp_chr2_63058206_F TCGTCGGCAGCGTCCCCTAATTGCCTGCACCAGA 60.0 0.00
mm4__chr2 63.06 Mbp_chr2_63058206_R GTCTCGTGGGCTCGGTGCAGGACACGGATCAGTTT 59.6 0.00
mm4__chr7 75.99 Mbp_chr7_75988833_F TCGTCGGCAGCGTCGCTGGAGGGGAAGAAGGC 59.7 0.00
mm4__chr7 75.99 Mbp_chr7_75988833_R GTCTCGTGGGCTCGGTTCCCCTCCACACACCCA 60.0 0.00
mm4__chr7 75.99 Mbp_chr7_75988808_F TCGTCGGCAGCGTCGCTGGAGGGGAAGAAGGC 59.7 0.00
mm4__chr7 75.99 Mbp_chr7_75988808_R GTCTCGTGGGCTCGGTTCCCCTCCACACACCCA 60.0 0.00
mm4__chr16 28.32 Mbp_chr16_28320006_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 28.32 Mbp_chr16_28320006_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 21.92 Mbp_chr4_21923666_F TCGTCGGCAGCGTCTCAAGTTCAAGGGGCTCATCT 59.0 0.00
mm4__chr4 21.92 Mbp_chr4_21923666_R GTCTCGTGGGCTCGGCAGAGAAGGATGAGCCCACG 60.1 0.00
mm4__chr7 92.25 Mbp_chr7_92246197_F TCGTCGGCAGCGTCGAACATGAGCCGGGCTTGA 60.3 0.00
mm4__chr7 92.25 Mbp_chr7_92246197_R GTCTCGTGGGCTCGGCGAGGCTCAGACTGGGTG 59.4 0.00
mm4__chr2 159.16 Mbp_chr2_159160967_F TCGTCGGCAGCGTCGCCAGGACCTGAGTGTTACC 60.0 0.00
mm4__chr2 159.16 Mbp_chr2_159160967_R GTCTCGTGGGCTCGGGCTCTCACTGGCAGACATGT 60.0 0.00
mm4__chr10 7.51 Mbp_chr10_7508399_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 7.51 Mbp_chr10_7508399_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529557 CTCCCCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCT
TGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCA
mm0__chr5 131.66 Mbp_chr5_131660016 ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG
CAGACCTTCCACA
mm1__chr2 73.90 Mbp_chr2_73901391 AGGCTTCGCAGGAGATGATGCCCCCCGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTCGCCACCAGGTGCGTGCTCATC
TGGATA
mm4__chr20 63.31 Mbp_chr20_63305079 ATAGGGTAGGAGCCTGTGGGAGTGGGCAGGGCCCTGGCCCCGGACTCTTCCTTCTTGGACCTGGCCCCTCGGGTCGTCAT
CCCCTCCCTGGGCAGCCCTGTCTTCATAC
mm1__chr11 1.80 Mbp_chr11_1803995 CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG
ACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT
mm1__chr19 9.52 Mbp_chr19_9518226 AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
GGCATCTGAAGCACCAGGGCGTTATGGTGGGCATCGGTCAGAAGGACTCC
mm2__chr22 15.72 Mbp_chr22_15721563 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm2__chr2 131.26 Mbp_chr2_131263632 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGCGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.66 Mbp_chr2_130656826 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.46 Mbp_chr2_130463843 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr2 130.08 Mbp_chr2_130075271 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.40 Mbp_chr14_19402808 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.00 Mbp_chr14_18998944 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm4__chr19 50.23 Mbp_chr19_50231971 GACCCCACTCATTGTCCCTGCAGCCGACCTCCTCCTCGAGCCCTGCTCCCACTACCGGTTCCTGACCAACGGGCCGTCAT
CCTCTCCCGGCCAGGAGCGGGAACTCTTCCAGGAGACGCTG
mm3__chr5 98.34 Mbp_chr5_98338819 GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT
CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm4__chr15 34.53 Mbp_chr15_34528430 TGGCATCTCTCTCTCCGGTACTGGATGAATCCAAGTTCCAGTTTCTCCACATGCTCCCTCAGGTCCGCCTTCTCCTCCAG
GAGGTCCATAAGGCCGCTCTGGAGCCAAAATAATGGGGTCACATCTCAGCAG
mm4__chr15 34.38 Mbp_chr15_34382209 TGGCATCTCTCTCTCCGGTACTGGATGAATCCAAGTTCCAGTTTCTCCACATGCTCCCTCAGGTCCGCCTTCTCCTCCAG
GAGGTCCATAAGGCCGCTCTGGAGCCAAAATAATGGGGTCACATCTCAGCAG
mm4__chr15 101.77 Mbp_chr15_101772440 CGCTCACTGATGGCTTCTGTCTTTCCAGTACTGGATGAATCAAAGTTCTAGCTTCTCCACTCGCTCCCTCAGGTCCGCCT
TCTCCTCCAGGAGGTCCATAAGGCCACTCTGGAGC
mm3__chr22 41.08 Mbp_chr22_41075139 GGAGTCCTTCTGACCCATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGA
CATCGTCACCCGCGAAGCCGGCCTTGCACATGCCAGAGCTGTTGTCAACA
mm3__chr1 223.86 Mbp_chr1_223864751 TGACCCATGCTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACCATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCC
TGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGAGCG
mm3__chr5 80.30 Mbp_chr5_80302747 CTCTTGCTCTGGGCCTCATCCACATAGGAGTCCTTCTGACCCATGCCCACCATCATGCCCTGGTGCCATGATGAAGGGGA
AGGCAGCCCGGGGGACACCATCGCTCGCAAAGCTAGCCTTGCATATGCCA
mm4__chr9 137.40 Mbp_chr9_137403842 CTGGGCTCTTCACCAGGAAATGAAGGGGAAGACAGACCTGTGGGATAGGGATGGGTCCCGAGGCGGGGCAGTCACACCCC
CACGCCATCTCTGCATAGACCCC
mm4__chr22 46.55 Mbp_chr22_46550559 GAAAGGACTGGAGGTGAGGCTTGTGGAGGGGAAGGCAGACCTGGGGCAGGAGGGAGGGATTCCAGA
mm4__chr2 131.63 Mbp_chr2_131626215 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm4__chr22 22.72 Mbp_chr22_22721287 TCATCCTCTCTCTCCAGCCCCAGCAGGACTGTGAACAAAGCCATAAACAGGGCTGGCCCAGTTCACCTGCATCTGAGACC
TCCAGGCTGCCTTTCCCTCCATTCCTCCAGGTAGGCTCTGCAGAAGGTG
mm4__chr3 32.24 Mbp_chr3_32238462 TGACAGAAGTAAAGGCCGGGAGGGGGAGCGGAAGACGGCCAGAAGGACCCCTGCGCCCTCTCCTCCGCGCCGCCCGCCAG
GCTGCCAACCCAGCCACCCTGG
mm4__chr7 43.13 Mbp_chr7_43131947 TGGCACAAGTGTTCACCCTTTGTCCTTCTGTCTCCTCCTGCTGTCTTATGACCGAGGATTGAAAGCTGCATGCTGAGGAC
TCCACATGGAGGGAAAGGTGGCCTGGGGGCCTGGGTTACAAGGGCATCGA
mm2__chr5 57.48 Mbp_chr5_57482604 ATCTCCCACGTAGCAGTCCTTCTGGCCCATGCCTACCATAACGCCCTGGTGTCGAGGACGCCCTATCATGGAGGGGAACA
CAGCCCGGGGGGCATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGACCCAT
mm4__chr18 79.54 Mbp_chr18_79544407 CAGCTGGACCAAGGACAGGGGGCTGGAGGGAGAGGCGGCCCTGAGGCCAAGCCCAGGGGCTCCCTAGGACAGGGCGGGCC
AGCCCTTCCCCTGGCAGCCCTTCCCCTCTCTGCTCCTCACCCTCTCTCT
mm4__chr2 239.39 Mbp_chr2_239392591 CAAGCAAGGAGGCACAGTTGGCATCACTAATTAAGATACACCCCGCTTGTGTGGACCCACAGGCCATCTTCCCCTCCTTG
TCCACAGCCCCCAGAGGTGGAATGGGGGTCAGCCTCCTTAGAAC
mm4__chr17 45.83 Mbp_chr17_45830610 GTTCATCTGCATTGGCTGGGGTGAGCTGGGCAGCCACCTCCGCAGCCTGGGCAGTGGCGGCCGCCGGGCTGCCCTCTCCT
CCAGACTCAGGCCAGCGGGCTGGGGGGCCTGAGGGATGGAGGTCGGGTT
mm4__chr19 11.02 Mbp_chr19_11022228 GCGTCTCTGACCCTATGCTCCAGGTGCAGAGTGGATGGTGAGGAAGATGGCCCTGAGGCAGCTGTGGGACCGCAAGCGTG
TGGAGAGCACAGCTAGAGG
mm4__chr1 22.01 Mbp_chr1_22009860 CTCGAGTCTCCGCCTTCATCGACTGGATTGAGGAGGTGAGGAGGGCAGGGCGGCCCGGAGGGCTTTAGGGTGGTGGCTCT
T
mm4__chr1 21.99 Mbp_chr1_21986686 GGTGTTCACTCGAGTCTCCGCCTTCATTGACTGGATTGAGGAGGTGAGGAGGGCAGGGCGGCCCGGAGGGCTTTAGGGTG
GTGGCTCTT
mm4__chr4 145.18 Mbp_chr4_145179760 CTGCCTCCCCACCCAGACCCGCCGGGCCGTCCCCGCCTCCCCTCGAAGCCCTCCCCGCCCCCCCGCAATTACCTGCTCCG
CCACGGCCCGGAACAGGCACGACCCGTCCTTGGCGACCAGTTTCCGATAC
mm4__chr16 71.54 Mbp_chr16_71537294 AAAGACCCCTCCCTCAACCTGCATATCGTGCACCTGGTCCGGGACCCCCGGGCCGTGTTCCGTTCCCGAGAACGCACAAA
GGGAGATCTCATGATTGACAGTCGCATTGTGATGGGGCAGCATGA
mm4__chr10 129.19 Mbp_chr10_129192599 TGGTGACAGCACAGCAGTAGCACGGCTGGCCATTTCCTTCTTGTCCATCAGGCCGTCTCCACCTCCATCTGGGGGGCAGA
TTGGATCTTGCCA
mm4__chr8 84.95 Mbp_chr8_84948665 CTCTGGTATGTGCAAGGCCAGAATCCTTGTCAAAGATGCCCCCAGGCCTTCTTCCTTTCCATAGTGGGATGCCCATGGGA
CCAGAGTTTGATGATGGGCATAGGGCAGAAGGACTG
mm4__chr1 229.43 Mbp_chr1_229433009 GCAGCCTGACCTGGTGTCGGGGGCGGCCCACGATGGACGGGAACACGGCCCTAGGGGCGTCATCCCCGGCGAAGCCGGCT
TTCACCAGGCCGGAGCCATTGTCGCACACGAGGGCGGTGGTCTCGTCTTCGTC
mm4__chr1 147.59 Mbp_chr1_147591707 TTCAGTCTCTGGCTTGGCTCCTTGTCTCTCCCTCCTGGCCATCCTCCCCTCCTGGAACTGCCCAAATCCTATTCTTCAAG
CCAGTTTGGCTTCTGATCCT
mm4__chr2 46.66 Mbp_chr2_46656422 TATGTCACCACCCCTGCCTACCCTCTGCCGCAGCCACTGTAGCCAGCGTGCCTCCACCTGGCTGTCTTTCCCTACACAGC
CCGCATGCCCCCCATAGCTGGCTGCACTATGGGAAGGACTGATCCCCACCAGA
mm4__chrX 103.38 Mbp_chrX_103377541 AACGAAGAGATGGAGCAGCCTATGCAGAATGGAGAGGAAGACCGCCCTTTGGGAGGAGGTGAAGGCCACCAGCCTGCAGG
AAATCGACGGGGACAG
mm4__chr7 50.40 Mbp_chr7_50399803 CGTCAGTATGGTGCAGGTGTGATGTGTCCGCAGGTGTGTGTGTATGTGTGCAGGTGTGGGGTCCGCAGGCGTGCTGGGCC
CCCAGGCCGTGTTCCCCTTCCCCTCCCCGGTTGTAGATTTCAGCT
mm4__chr3 38.01 Mbp_chr3_38009732 GCCTCATCCTCCATCTCAGCAGCCTCGTCTTCGTCTGACACCACAGTGGCCTCAGGGCCACCCTCCCCTCCAGGGGGCAG
GAGCCCCCCGAGCTTCTTCCCCTTCAGCAGGATCTTCCCCTTCAGTTGCTAGGTGG
mm4__chr5 60.21 Mbp_chr5_60210813 CCAGGTAGTTCTGGTAGGCTACAATGTAGGGGAAGATGACCAGGTGGCCTCAGGGACCAAGAACAGTTTCACTTTGGTGC
TTCTATTTCTAACAGCTGCGTTTCCATCCTAATGGTGGATGCTCTTTTATGGCAGGCCA
mm4__chrX 48.56 Mbp_chrX_48558825 TTGTTTGTTCCCCTGCAGGTAGCCCGGCTTCAGTAGAAGGAGAGGAAGCCGGCCCTGAGGTCTGGGTTATCATCCCCATT
CTTATAGCACTTTGTTCTGTTTGCCCAGGCCCATTGCTAGAAG
mm4__chr1 206.38 Mbp_chr1_206381341 GGGTAAGCAACAGGGTAGAGGAATAGGAGCTTTCTCCTTTAACCTCTGCCATTCAAACATAAGGAGGAGTTAAGAAACGA
TAGGCTGGCAGGGGGAGATGGCCCGGAGGCAGGAGGGCAGGGCGGTGGGAAGTGATACTC
mm4__chr1 144.92 Mbp_chr1_144917402 GAGTATCACTTCCCACCGCCCTGCCCTCCTGCCTCCGGGCCATCTCCCCCTGCCAGCCTATCGTTTCTTAACTCCTCCTT
ATGTTTGAATGGCAGAGGTTAAAGGAGAAAGCTCCTATTCCTCTACCCTGTTGCTTACCC
mm4__chr1 204.13 Mbp_chr1_204131754 TGTGATGCTGGTTTGGAGCTCAGCACCTCCCACATGGGGCATGCAGGGGGAGACGGACTGGAGGCTCAGGGCACTGGAGG
TAGAAAGGAGCCCTCCCCGCCTCCTTCCCCAGGCCAGGAAGGGGTAAACACA
mm4__chr20 61.66 Mbp_chr20_61658196 TGCTCTCGCTTCCAGATCTCTCTATTAGAGAGGGTTCAAATGCATCTGCAACCCCCGAGCCATCCTCCCTTCCAAGTTCC
AGATCTCTATTAAAGAGGGCTCCATCGCAC
mm4__chr12 90.07 Mbp_chr12_90070769 TCTTAGCCTCAGGGAAAGCCCTGTATCTACACAATCATATCTCCCTTTCCTAATCAGCATTCCTATGCAGGGGAAGACAG
ACAGGAGGAGGCAAGTAAATCAAAGAATCAAGACTTAGTGCTGGGATGTAAGACACCG
mm3__chr14 99.25 Mbp_chr14_99254717 TCATGGAGGGATTGGAGGACTCCTTGGAGCCCCGGCCAGGAGGCAGAAGCCTGGAGGGACAGCCGGCCCGGGGGCCAGGA
GCGTGGGCAGCAGCTGGACGGGTTTCCGGAAGCCTGGGCGGAGAAGTGCCCGTGATTCCT
mm4__chr12 130.43 Mbp_chr12_130425332 ACAGTTCACCAGACACGGAGCCTGACCCGTCCCTGTCCATCCACTGGGCCAACTTCCCTTCCAGCGCTCGGCACCCTCTG
CAGGACTTTCTAGTTAGGCTGACGTGTCACCCTTCTCC
mm3__chr11 111.22 Mbp_chr11_111221709 GCCAGGGGCTCTTAAGTATGTTTCTGCTTCCGTACAGTTTGTGGAGGGGAAGACAGCCCCCAGGGCACCCATGAAAGCAG
AGAG
mm4__chr6 165.79 Mbp_chr6_165789069 CTGCTCTTCTGGCTGAGTGGAAGGAGGCCGAGGGAGGGGCAGAGCATCCTCTGTGGGGGCTGGAGGTGAAGATGGCCATG
TGGAAATGACCCTGACACAGACACTGAGACTGTAGGGTCTGGAGTTCAGGGTTG
mm3__chr3 12.07 Mbp_chr3_12070301 TCATTGACAGTGTCTCCGGCATGTACAAAGCTGGATTTGCTGGGGACGACGCCCCCTGAGCCGTGTTCCCCTCCATCCTT
AGGCGCCCCTGGCACCAGGGCACGATGGTGGGCATGGGTCAGAAGGACTC
mm4__chr10 117.38 Mbp_chr10_117375718 GTGGCCGTCACTTCTGGAGCTGCGGTCGGGATCTGGGAGGACGGCTGGGGACGCGGGCTGGAGGATAAGGCGGTCCGGCG
GAGCGGGCCTGACGCGTTCCGGATGCCGGCCATCCCGTTATCCTC
mm4__chr16 4.68 Mbp_chr16_4681608 ACAGAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGCTCAACGGCCTCCGGGCTGTCTCCC
CGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGGCATCTCGGACGG
mm4__chr8 125.48 Mbp_chr8_125475321 TGGAGTAAGGCCAGAGGTCAGCATTTGGAATGGCCTTGGAGGGGGAAAAGGCCAGGGGGAGCTGGGTGCAGCCAGGAGAG
GCAACTAAGGAGAGGGTACCCTGCACACCGTTACTGACT
mm3__chr9 126.03 Mbp_chr9_126028190 ACAGAGCTCTCATCTCCCGATCTCTGCCCCCCAGGCCTTCCTCCCCTCCAAGAGTTGNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNAAGCAGCTGAACATTTAGTGAGCACTCACCCTACACTGAGCTCAGC
mm4__chr1 50.15 Mbp_chr1_50152174 CCCAGGATGTCTGTAGTCAGGATTACAGACCAGGTATGGTTTCCTTTTGCCTCCTGGCTGTGTTCCCCTTCATCTTACCT
TCCAAATCAGACAGTTCTGCCCTGCTTCC
mm4__chr22 21.55 Mbp_chr22_21548576 CTGTCAGGCTCCAGGTCATCCACCTCACTGGCTGTGTCCCTGTCCAGAGTAGGGGAGGACGGCACGGTGGCCCACATCGA
CCCCTCAGAGGAGGAGTTTGACTGGGAGC
mm4__chr22 18.61 Mbp_chr22_18609034 CTGTCAGGCTCCAGGTCATCCACCTCACTGGCTGTGTCCCTGTCCAGAGTAGGGGAGGACGGCACGGTGGCCCACATCGA
CCCCTCAGAGGAGGAGTTTGACTGGGAGC
mm4__chr7 155.79 Mbp_chr7_155788778 CTTGGACAATGAGCAACCGCAGCCGGCCGCGCAGCCTGGGGGCCGTCTCCCTCTCCAGCCCCCGTGTCCTCTGGTCCACG
ACTGGCTCTTGCCCTCATTCCGTTGGGGCTGAGTCACAGCCAGGGAAAGG
mm4__chr19 14.57 Mbp_chr19_14565698 TGGATCGGTTTCGCCATCATGACGCAGTGTCTCCCAGGTGAGCCTGCCGCCCTCCCTCGGCGGGGCCCTGCCCCTCCGGG
CCGGCAGCCCCTCCCTGACGCCCGTTCTTTCCTGCAGTGGCCC
mm4__chr6 31.14 Mbp_chr6_31142556 CACCCACTTCTCCAGGATCCTCCCAGCCCCCAGGCTGGCTTTCCCTCCAACTGTCAGCTGCTTAGCTGCTCATCTGGGGA
TTGGAGCTGGAGCATCTGTCAAGGT
mm3__chr6 46.21 Mbp_chr6_46205052 CGGCATGTGAAAAGCAGCTTTGCTGGGACAATGCCCTCCGAGCCATATTCCCCTCCATCATCGGGCACCCCCGGCACCAG
GGCGTGATGGTGGGCATGGGCCAGAAGGACTCCTACGTG
mm4__chr8 12.95 Mbp_chr8_12951418 GTCGTCGTCACCCAAGGTACTGCGCTGATGCGCTGCGGGCCGACCAGGTGCTCCCGCCGGGGCGTCTTCTCCTACGCAGG
AAGGGCCACGCCGAGAGAGGCAGGCAACAAGG
mm3__chr9 2.02 Mbp_chr9_2016820 CCCAACCTGGTTTACCCCTTCCTTTGCTCTCCCCCTCCACCCGGCCGTCTTCCCTTCCTCCCGTTTGCGTTGCAAAACAC
GGCCATGCCATCTGCAAAGGTGTCGCGATGCACTTCCCTAAA
mm4__chr19 44.82 Mbp_chr19_44820412 CTAACCTTGTCTCCAGCCCCAACCACATGGCCGTCCTCACCTCCAATCCGTAACCATCCCAGACCGATCCCAAAGCCAAC
TCCAGCTA
mm4__chr7 5.44 Mbp_chr7_5439985 CCACGGTCATCAGAGCCATCGGGGCCCTAGGGCTCAAGGGCAGGGCTGGGGCCCTGGAATTCCCACAGGTGCAGGGGATG
ATGGCTCGGGGGTGGGGAACAGAGGGGCCCAAGGAGTCAACAGCACCT
mm4__chr9 130.32 Mbp_chr9_130318968 AGAAGGGGCTGTGGAAATGGCAGCTGAGTCCCGCCAGGCCCCCCAGGCCATGCTCCCCTCCACAGAAGTGGGCCTGGCTT
AGGCCAGGGGCTTAAGCATCAGAGGGGAGCTCAGGACACCTCCAAGA
mm4__chrX 53.14 Mbp_chrX_53143812 CATGGCCCATGTAGGAGTCCTTCTGGCCCATGCCCACCATCACACCCTGGTGCCGGGGTCACCTGATGATGGAGGGGAAC
ACAGCTCAGGGGCCATCCATCCCCAGCAAAGCCAGCTTTGCACATGCCAGAG
mm4__chr14 96.77 Mbp_chr14_96772334 GGAAAGTATCAGGGGCCTGGCTCTGGGCCTGTGCTGCCTGGTGGGGAAGTCAGCCCTGTGGGCAGGGCCAGTTGGCAGGT
GCCAGGGCCTTTCTGAAAA
mm4__chr19 16.54 Mbp_chr19_16542976 CGACGAGCTTGACTGGGATTGCAGCTGGACGTATTTAGGTTCAAAACATCCTCCTCCTGGCTTTCTTCCGCTCCACTCTT
TGGTCAGGAAGACTGGGGCGGGGTACCCCCCAACCCATGCCCTCAAATTGCTG
mm4__chr19 19.26 Mbp_chr19_19258450 GGTCTCCTGTTTCTGATCGCTAAGAGCTGGGTGGGGAAGAAGGCCCCGGGGTTGGGGTAGTGTTGCAGCAGAGGCCAGTC
TTGGGGTGAGGCCTACGCACCCGGCAGGTTGGACGTGAAGCAGAAGGCGTCGTAG
mm4__chr19 38.51 Mbp_chr19_38512046 TGGGGTGGATGTAGAGGGAGGCACTGTCCTCTGTCCTCTTAGCCATGGCATCCCCCCGGCCCATCTTCCTCTCCCAGGGA
CAGACGCCCCAGCTGTGGTCAACTGTCTTCACATCCTGGCC
mm3__chr12 49.30 Mbp_chr12_49295220 CTAGCTCTGCGAACGGTGACTGCCCATCCTTGGCCGCAATGAGCCACCACCCGTCGGGCCTCCGGGCCGGCTTCAGCTCC
ACCTCATACCGCCGTACCTTC
mm4__chr7 100.66 Mbp_chr7_100655570 TCCCTTTCTTCTCTTTGTCCCCCTCCAGCTCCAGCCCGCCCCCCTCCTCCGCGGCCAGCAGCCCCACTGTGGTGGGGAAG
TCAGCCTGGTGGGGAAGGGTTGGGGGAGTATTGGCAGGGAGAGAGGTCA
mm4__chr15 26.89 Mbp_chr15_26891558 TATGCGCGCTATTCAGCCATGGGGACTGAAAATAATGTCCACGAGGTGGCGCCGTGGCCTCGTGCATCGAGGGGAAGACT
GACAGGAGGGCATTCTAGAGCTTGGTTAAAGCCCCGGATTCACCGAAGCACGTTTAGGG
mm4__chr6 158.82 Mbp_chr6_158819603 AAGTGGGGAGGCTGTTTGTCCCACAGCGGGCAGGGGGAAGTCAGGAAAACGTAAATAAATCTGGAGGGGCGGACGGCTTG
GGGGAATCGCTGGACCGCTTGG
mm4__chr3 50.47 Mbp_chr3_50473414 CCCTCATGGCTCCAAGCTACTGGGAGTGGGGCAGCCTCTAGGATGGAGGCACGCAGGCTGGGCAGCCTTTGAGGGGAAGA
AGGCCAAGGGGCCTGGATGCCAGATCACCCCAGCTGTTT
mm4__chr2 109.45 Mbp_chr2_109446808 TCTTTGGTGCCTGGGATGAGCAAAGTAGTGCGGCAGGCGAGGGGAAGCCGGCCAGGAGGTAGAGGCCAGCCACGCACAGC
CCAGGGGCACAGACCCTGGTGCAGTC
mm4__chr17 42.80 Mbp_chr17_42796800 ACTGAGCAAGGAAGATCCCAACACTGAAGGGGTAGAAGGCCAGGGGGGCATGGAGAGTGCAGCTCCATTATAGTGAAGAG
CCAAACATATGTGAACTGTTTGCTGTGTGGAGGTGT
mm4__chr11 40.29 Mbp_chr11_40293454 CAGATCCTCCAGGTGTTGCAGAACTCCGCGGTCGGGGCCGGGATCACGGGGGAGACGCAGGATGCGGTCCCTCGCCGGTG
GCCCCTGCCAGCCCCCGGGTCCCCTTCTCCTCCATCAGCCGGTAGTGGCTCCGGGAG
mm4__chr11 70.75 Mbp_chr11_70745446 GGGCTCACAGTCCCTCAAAACAAACCTCCGGGCCTCCGTCCCCTCCTCCTGCTGCCTTGAAAACACAGTCTTGGCTGTGT
CCCTTGGCTCTGTC
mm2__chr5 138.25 Mbp_chr5_138252936 CTGCTGCTGTCCTTTCCTCACCCCTTGTGGGCTGCAAGTCCACCTGGGTGTGGTGGAGGGGAAGAGGGCCCTGGGGAAGT
CCAGCTACCATAGGCTCAGGAGCAGAATCAAGGGAAGCGTGCA
mm4__chr6 101.43 Mbp_chr6_101431437 TGATGCTGTCCTGTGTGCTTACTAGAAAGACAAGTCTTTACTCTGACAGAGTTTATATTCTGGTGCTGGGGGCGCCCAAC
AATGGAGGGGAACATGGCTTGGGGGCGTCATCCCCAGCAAAGCCG
mm4__chr19 19.32 Mbp_chr19_19321008 CTGAGCACTTCCGCACTTCCAGCCCGCCCAAAATCCGCCTGTGCGTGCACTGCCTGCAGGCCGTGTTCCCCTTCAAGCCG
CCGCAGCGCATCGAGGCCCGTACACACCTGCAGCTGGGCTCCGTTCTCTATC
mm4__chr3 128.85 Mbp_chr3_128850660 GTCTTGCCCAGTTCTCCCATGCGGCCGTTGGAGCCCACACGTGGCTGACCTGTCATGTTTGTAGTGTTCTTGGACCTCAG
GGCCTTCGTCCTCTCCATTCTAAGTACAGAAAGACATTTGACTGACATGG
mm4__chr13 113.08 Mbp_chr13_113083520 TGCTCATGCCCCAAATCCATCCCCCAGACGGTTTTCCCCTCCAGCCGGACTTCCTCCAGGGGACAGATGCAACAGAAAAC
AGCCCAGG
mm4__chr7 153.94 Mbp_chr7_153935009 AAAACAAGGCTTCGGCACTGAGGTAGCGGAGGGTCCGTCATAGCCCAGGGTGCCATCCTGGCTGGAGGTGCAGGGACAGA
AGGCCCGGGGGAGGAGCTCAGGGACCAGCCCCAGGGATGGCGAATGTCCTGACA
mm4__chr14 31.95 Mbp_chr14_31951620 CCCTCCAGTCCCCTCATAGAGTATATCCTCCCCCATGCCTCCTTCCCCTCCACGCCACATGCCCCAGGCTCCCTGAACAT
TACCAC
mm4__chr1 109.48 Mbp_chr1_109484842 CATCACGCCCCTGAAGAAGTTTGCAAGTGAGAAGAGGTGGAGGAGGAGACTGCACGGAGGGGTCTCTAATGACACTTGTT
CTCCTCTGAGACATCCGACTCCCAGTTTCG
mm4__chr12 9.16 Mbp_chr12_9156321 TCTGCTTCCAGTACCTTCGCTTTGGGAAACTTCAGTGAAGGCATCTCCACCAATAAGCGAGTCATCAATGTCACCCTTTG
TCCTACTGACCATCTTCCCCTCCACCTCCAGGCACAGTCCTTCCTCCATC
mm4__chr17 81.04 Mbp_chr17_81038556 GCCCTGGCTGGTCACTCTAGCAGCTGGTGGGGAAGCCAGCCTGGCGGGGCCGCAGCCGTGCTTCGAGGAGACGGGTGCCA
CAGGGTTCCTGGAGCCAGGAGCCAGTTGGGTTTGCCGCCTCTGGTGTGATTGGTA
mm4__chr10 45.30 Mbp_chr10_45303669 GCAGGTGGAGAAGGCTTTCTGCCTCCCCCAGGCAGTCTTCACCTTCAGGATGCTGGAGACGATGAAGCCATAGGACGCGA
mm4__chr2 19.80 Mbp_chr2_19799444 GCCGAAGAGTCCAAAACAGCATAATGGGTGCTATGATGGAAGGGAAGAAGGCTCCGGGGGAGGGACAAGGGAGAGGAGT
mm3__chr5 154.59 Mbp_chr5_154590930 TCCCTGAAGCCTGGAAGGATAGGTTCAGACCACAGGGCCCTCTTCCCCTTCAAACATCTAAGCCCCTCATTCACCTGGTG
CCCTGCCATTCACTGCAGACTTCTCCGAATGGTTTCCTGGC
mm4__chr9 1.05 Mbp_chr9_1048247 GGAACAGAGGCTTACACCCCTTTCTCCTCCTGTTTCTCCTCCAGTCCCTCTTCCCCTCAATCTAGTTTCCCAAAGTTAGC
AAACCTACTTTCTTGATTTTGTTTCTTAACATTTAAAACAACTGGTGAAGGGCT
mm3__chrX 44.77 Mbp_chrX_44765430 ACCTCTTCTGACCCTGGTGTCTCCCAGCTGTGTCCCTGGCTATAGCCCACACTGCACATATTCTAGGCCACCAGGCCCTC
TTCCCCACCATATTAGTCATCAGCTTTCTATTGGCTTTCCTCTCCCCAGA
mm4__chr12 131.85 Mbp_chr12_131846283 CATCCAGTGTCACCCTCAGCCACATCCCTCCTGCCACCGCCTCTGTCTCCACCCGGCCGTCTCCTCCCCCATCTCCGCAT
CTTCTCTTATAAGGAAGCCGGGCCTTG
mm4__chr13 99.89 Mbp_chr13_99894959 CCGTCTGATGTCCCAGTGTTAGTCCTCCCGGCCGCCCTCCCCTCCTCTCCTCCAGGGACGGCTGGGAAAACAGTCCTGCG
GGATGAAGACTTCACCGCCTCCGATTTGAA
mm4__chr16 173.64 Kbp_chr16_173641 CACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCCAACGGGCCC
TCCTCCCCTCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCAGCAG
mm4__chr10 132.11 Mbp_chr10_132108474 ATCTCCTCAGCTTCCAGGGTGTGCAGCTACTGGAAGCAGGGGCTGGAGGGGAAAAAGGACGGGTGGGACACAGGTGGAGC
CTCAGACAAGAG
mm4__chr18 37.42 Mbp_chr18_37423402 GGAAAGGAGTCCTTGTGGGGCCAGAGTGGGATGGGAAGACTGCCTGGAGGAGGCGGGGTCAAAGGTGGGCTTAGGAGGAG
CTCAGAGACATTGTTGGTAAAAGGAGGGGCCTGAGTGAGGCACCCTTGAAGTCA
mm4__chr1 224.50 Mbp_chr1_224501967 AGGAGGCTGGAGGAAGGTAGGGTGGAGGGGCAGAGGACCTGGAGGCTTCATGAGCAACTGGAGGGAGGTGCTGAAGAATG
TGGGGA
mm4__chr16 169.38 Kbp_chr16_169384 TGTGAGTGCTGTGTTGACCTAAAAACACCATTAAGCTAGAGCATTGGTGGTCATGCCCCCTGCCTGCTGGGCCTCCCACC
AGGCCCTCCTCCCCTCCCTGCCCCAGCACTTCCTGATCTT
mm4__chr14 104.68 Mbp_chr14_104681991 GAGCTGTCACTCTCCCAAGGCGGGAAGTGGGAGGGGCTGGGAGTCTCCAGGGAGGGGAGGAGGGCCTGGAGGAGTTCCCT
GGGGTGGGGGTCCCAGAAGAGCCAGCAG
mm2__chr18 62.44 Mbp_chr18_62442085 CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA
TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC
mm1__chr6 88.28 Mbp_chr6_88276907 GAGTCCTTCTGACCCATGCCCACCATCACTCCCTGGTGCCTAGGGTGCCCCACAATGGAGGGGAAGACGGCCTGGGGAGC
CTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGCATCATCCA
mm4__chr1 13.78 Mbp_chr1_13780694 GCTGCCTCCTCTCTTGATCCCCACAGATCCCTCTTCCCCTCCACCCTGTCCCCCGGTATTAACTGTTGCCACTCCGCCCC
CTCCCCTCCTTCCTACCGTACCTCTTCCAGCCCCCTCTTCCAGTGCATCTCCACACCCAT
mm4__chr1 226.38 Mbp_chr1_226380068 GCCACAACTTCAACAGGCTCTGCCTTCACCTCTGCCCCCCAAGGGGACAAGATGTGCGCTAAGAACAACTCCTGAAGGCT
CTTGGTGGAGGCGGAGACGTCCTGGAGGAAGTCCTCAGACACAACTCGGATGTTGGC
mm4__chr4 45.03 Mbp_chr4_45034164 GAGGACAAGGAGGGCCATTCAGCCAGTGGGCTGCAAAAGTGAGACTGTCACCACAGTGCCCTCTTCCCCTCCCTGGTGCT
GACAGCTTGCAGTCATGCTTGCGCTGTCTGCAAAATATCACCTTAGGGGAGTCTGTCTGC
mm4__chr20 57.53 Mbp_chr20_57526423 GGAATGAGGAGGGGAGGAGAGCCCTGTAGGGGGCGCTGGGAGACCTTGGGGGGAATGGATAAGGAGGGGAAGAGGGCCCT
CAGGGGGCGCTGGGAGACCTGGGGGGGAAATGGATAAGGAGGGGAGGATGGCC
mm3__chr6 42.78 Mbp_chr6_42782778 GTGTGAGTGTGTTTTCCGCCCCCCGGAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCCTGGAGTGACGGCGGGCGAGGAAGAAAA
mm4__chr20 24.07 Mbp_chr20_24066266 TCAGACAGGTGGAGGAGGAGCTGCCTACATTTCCTGCGGGGGGTGGAGGGGAAGTAGGCATGGCGGTGAGTCCTGGGCTC
TCAGACTTTGCT
mm4__chr9 113.88 Mbp_chr9_113876826 TGCAATTTTCCCCTGACCCTCTCTGCGCTCGGGGTCCCCGATGACCCCCCGGGAGGTCTTTCCCCCCAGGTCCCCCATTT
TTGCAACAATCTTTTTTTTTAGGCCATATTTTCCTTTCTCAATTT
mm4__chr15 69.39 Mbp_chr15_69393673 GGAGAGAACCACCTTCCCCTCCAGGGAGGGGAAGACCTCCTGGGGGAGACCAGGAACCTCAGTATTTGCTTGACAGTTAT
CTGATGCTTGCCCGAAAATCAGGGTTGA
mm4__chr10 4.19 Mbp_chr10_4188630 GACGGGGTTCATGGAGCTTATGACGGTTTCCCCAGTGGCATTCAGAGCGCAGCCTGGGGCCTCAGCTCCGTCTTCCCCTC
CCCAGCCTCGGGCGTCAATGAAGGCAGCGCTGGCCTGTTGCAATCAGACT
mm4__chr20 24.79 Mbp_chr20_24787469 TTCCTCCCCATACATTCGCCCCCACCTCCAGCCCTTCTTCCCCTCCCTGACACAAGCATCACCTTTAAGGTCTTTCCTGT
GTCTCTTTCTCTCCAATGCAGGAGGACTCCCTCCCTCCATGTCT
mm3__chr7 155.35 Mbp_chr7_155350237 TCCCTCGGCTTCCTAGAGACCCGGCTTCCGGCTTCCTGCGGTCTCCCGGGCCGGCCTCCCCTCCCGGCTTCCTGAGGTCT
CCCCGGCCGGCCTCCCCTCCCGGCTTCCTGCGGTCTCCCGGGCCGACTTC
mm2__chr3 175.98 Mbp_chr3_175978479 GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC
ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGC
mm4__chr12 48.89 Mbp_chr12_48890981 GCGACTGAGGTCAGAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCATCGCCCACCGGGC
AGTCTTCCCACCGACGATTAGGCTTTGTCTTCAAAACAAGTTAGAGGTGGTGGCTTCTGC
mm4__chrX 150.69 Mbp_chrX_150693807 GGCGGCCCCTCTTCATCCCCTCTGGGCCCTCCTCCCCCCCACCCCCGGAAACCTCAGCGCCCCAT
mm4__chr3 94.14 Mbp_chr3_94140001 AGGTGTTATATGCCATGTCATTCAAAGCCTCCCTGCTGCCTTCCCCTCCAGCCCTACCCCGTGTAGGCCTGTAA
mm4__chr3 135.84 Mbp_chr3_135839632 CCCAGCCTCCAAACCAATGAGAACCTCCCTTTCTTCTCCCCCGGGCTCACATCCCCTCCATGTGCCCCAGGACTTGACCA
GTTGTTGCCCAAAAGCAAGCTATACCACCAGGTTGCCTCCTGAGATGCTGCTCTACCCCT
mm4__chr12 13.83 Mbp_chr12_13833369 TGGCTTAGGACAGTCACAATGGGCCTCGGAACCTTCTTCCCCTCCATCTCCTCTGCATATTGGCCTTTTGCTTATTCCAG
TTTTGCTCTCCAGCCCTTCCCTGATTC
mm4__chr15 63.91 Mbp_chr15_63905629 GCCATCCAACACCAGAAAGCTGGGCTAAGAAGACTCAGTCAGCAGTGCTTTATATAACCTGGATGAGAAGAGGGCCAGGA
GGAGATATGGGCATGATGGAGTCCTGGTT
mm4__chr3 195.76 Mbp_chr3_195763321 ATTTACTCCGGGACAGCTGCGTGGATGGGCTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT
CTCCTCGGCCTCAGTATGT
mm3__chr18 79.77 Mbp_chr18_79771320 CATCCTCCCCTCCACGCCGTCCTCCCCTCCACGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTC
CACGCCATCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCAGCCTCCCCTCCATGACAT
mm3__chr18 79.77 Mbp_chr18_79771303 CATCCTCCCCTCCACGCCGTCCTCCCCTCCACGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTC
CACGCCATCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCAGCCTCCCCTCCATGACAT
mm3__chr18 79.77 Mbp_chr18_79771133 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm3__chr18 79.77 Mbp_chr18_79771116 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm3__chr18 79.77 Mbp_chr18_79771099 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm3__chr18 79.77 Mbp_chr18_79770997 CTGGGGTCGGAATCCAAAGGCGCAGGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACACAGGGCTCCCCTCCACACCG
TCCTCCCCTCCACACAGG
mm4__chr12 31.23 Mbp_chr12_31226362 GAGGGAATGCACCTGCCTAGGCTGGAGGGGAGCAGGGCCTGGGGGTGTGGGTGGTGTTTGCTATTCTTCCCAGGATCACT
GAGCACCTAGGTGAGCTGAGCTACCGGAGTCCTTGGAAAGACC
mm4__chr22 47.06 Mbp_chr22_47057335 AGCAGAAGTTCTTGCCTCCCACCCCATGGTCCCTTAGGCCACCGTCCCGCCTTCCCCTGCACTTTGCAGCAGGATTTTAC
AAGTTTCCTTCCAGGTGCAGGGAACCAGGGTGGCTCGAACCCAGATGGACAGTGCT
mm4__chr22 46.25 Mbp_chr22_46250688 GACTAGGCTGCCAGTCTACCCCACCGGGACGTCTGCCCCCGCATGCAGGAGGGGGTGTGTCGGCCCAACGGGGTCTCAGG
ACCTGTCCCTGCTCCTCCAAAG
mm4__chr13 73.06 Mbp_chr13_73059801 CGTTTCGCTGAGAGTAAATGGGGGGGGGGGCCGGGGGTGGGAAGGATGGAGGGGAATCTGCCCCGGCGGGTCGGCCTGGG
AGAGGTAAGAGGGAGGG
mm4__chr15 66.37 Mbp_chr15_66370475 CGGACGTGAAGAACCAAGGAAGCGGATGTCCTTGGTGGGGGGCTGGAGGGGAGGAGGGCCAGCAGGGGCATGGGGCGGGG
TGCAGAGGGGATAGTA
mm4__chr21 40.56 Mbp_chr21_40564940 CTAGTTGCTCCCTGTGCCTCCAAGGTGAGCACCTGGAGATTGGAAGGGAAGAGGCCCAGGTGGGATGGGCAGAGGTGGAG
GGGAGGATACTGCTC
mm4__chr1 33.99 Mbp_chr1_33985149 ACAGCTTCAAGGCTTCACCAGCAGGTCGATGGAGGGGAAGCTGTCCTGGAGGGATGGAGCCCTGGGCTGTCTCCCTTTCA
CTATGGAGACTCAAAACTCAGGACTTAGCCTTCCTTCCCCATCCA
mm4__chr19 15.45 Mbp_chr19_15445454 CTCCATCCAGAGAAGTGGCGGTGGAGGGGAAGAAGGCTGGTGGGAGGCCTGGGGGATCAGGGCTGGTTCAGCGATGTCTC
AAGGGG
mm4__chr5 43.00 Mbp_chr5_43001171 TCGCCTGGATTTGCTGGTTGCAGAGCAAAATCAAATACAAAAAGTGGTGTGTCTTATGCAGAGATTGGAGGGGAGGACCG
GCCCGGGGTCATGCAGGGTGGGGGACTCACAGGCTAAGC
mm4__chr20 49.48 Mbp_chr20_49482197 TCCTCCATCATGTGCAGTCGCCCAGTGCGGTAGAAGTTGAGGATGGAGGTGAAGGCGCCCGGGTGGCGGTCAAAGAAGTA
CTCGTTGTCGTCGAGGCTGTAGTCATCGCACAC
mm4__chr18 2.91 Mbp_chr18_2908702 GCTCCAGTGCCCATCTCTTTCTTTCCTGCAGCCAGCTTGCCCTCAGTGCCCTCTTCCCCGCCAGGGATAAATGGGCTAGT
GAGGAAGCAGGGACGGGCTGGTACAAATGTGCCTCTGCTTGAAGGAG
mm4__chr18 79.77 Mbp_chr18_79771337 CATCCTCCCCTCCACGCCGTCCTCCCCTCCACGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTC
CACGCCATCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCAGCCTCCCCTCCATGACAT
mm4__chr14 74.41 Mbp_chr14_74407620 CACCACAGCCACGTAGAGACCGGCCCCCACGGCGATGGCGAGTGTGGCTAGGAAGAGGGCCCGGCGGGAGGTGGTGCTGG
CCAGGCGGAAGTCCCCTTTGGAGATGGCCTTGCTGGTCTAGG
mm4__chr1 99.00 Mbp_chr1_99004161 CCCCAAAGCTCACCTCCTTTGCAACCCGGATCCCCACTCCTCACCACCTACGGCCCCTCTTCCCTTCCATCCCCGCCCAG
TCACCCAACGCTGAAGCCACCGCGGGGTGTGGGGGGGTGACGTGTGGGAAGAGCTG
mm4__chr1 4.06 Mbp_chr1_4058990 GTCACCACCTTGCTCTGGTTAGAGGCTGGATGGGAAGAAGGGCTGGTGGGGCTGAGGGGAGGCTGGCCCTGTCTTTTTGC
CTCAGAGCTGGGCAGCAACTCCCACATGAGTATTTAGGGGAAATGCTGGTCCCTTGCAGA
mm3__chr2 100.84 Mbp_chr2_100835853 GAAGTCACTCAGGGTCACCCCAAAACATATCCTCCTGCCCCTCTTCCCCTCCACAGCTGAGCACAGACCTCAGCACTCAA
mm4__chr17 16.42 Mbp_chr17_16417141 CAACATCAAAAGGGTGGCCCTATTGGTTCCCTGTTCCTCACCCCACAGGCCTCCCTCCCTCTCCTCACCCCTGCTGCTCA
TCCCTCCTGGCCCACTTCCCCTGCATTGGGGCTGGGCTCCCACACAGTGGGTGGACCTAC
mm4__chr1 10.83 Mbp_chr1_10834502 GCCAGTGAATCCAGGGAGACTGAGGCTAGAGCAGGGAAGGAGGGGAACACGGGCGGGGGGGGGTGGCACTGCATGGTATA
mm4__chr19 14.51 Mbp_chr19_14506107 AACAAGCTCCTGTCCCTTGGCCTGTGTGTCCAGGGCCTGCCAGGCGTCTTCCCCTCCACGCTGCGGGCTATGGGAAGTGG
CTCGGTC
mm4__chr18 79.77 Mbp_chr18_79771031 CTGGGGTCGGAATCCAAAGGCGCAGGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACACAGGGCTCCCCTCCACACCG
TCCTCCCCTCCACACAGGGCTCCCCTCCACGCCGTCCTCCCCTCCATGCCAG
mm3__chr20 61.80 Mbp_chr20_61800027 TCTCCGCTTGTCTTCATGGCCCGTCCCTTCCCATGACCCGCTCGGCTGCAGCGGCCCTCGGTGGGCACAGTCACCGGGCC
GGCTTCCCCGCCTGTGCTTCCTCTGAGGGGTTC
mm4__chr18 79.77 Mbp_chr18_79771524 TCCTCCCCTCCACACAGGGCTCCCCTCCACGCCGGCCTCCCCTCCATGCTGTCCTCCCATGGACCCGGCTCTCAGGCTGC
CCTCAGTCCCCGGCGTCCCAAGCCCGTGGATGCCTGCAGCTTCCCAG
mm4__chr2 63.06 Mbp_chr2_63058206 CCCTAATTGCCTGCACCAGAAAGAAGAGGGGGAGGGGAAGAGGGTCGGGAGGCGGGAAAGGATAGCTCTGTAATGCTCTC
AGAGCTGAGCCAAAAAGGAAAAGAAAGCAAAACGACAAAAAACTGATCCGTGTCCTGCA
mm4__chr7 75.99 Mbp_chr7_75988833 GCTGGAGGGGAAGAAGGCCGGGTGGGGGGCTGGAGGGGAAGGCCGGGTGGGGGGGTGGAGGGGAAGGCGGGGAGGGGGTG
TGGAGGGGAAGGCGGGGAGGGGGTGTGGAGGGGAAGGCCGGGTGGGTGTGTGGAGGGGAA
mm4__chr7 75.99 Mbp_chr7_75988808 GCTGGAGGGGAAGAAGGCCGGGTGGGGGGCTGGAGGGGAAGGCCGGGTGGGGGGGTGGAGGGGAAGGCGGGGAGGGGGTG
TGGAGGGGAAGGCGGGGAGGGGGTGTGGAGGGGAAGGCCGGGTGGGTGTGTGGAGGGGAA
mm4__chr4 21.92 Mbp_chr4_21923666 TCAAGTTCAAGGGGCTCATCTCCTCCACGAAGTCTTCCCCTCCAGCCACTCTAGCCCACCGTGGGCTCATCCTTCTCTG
mm4__chr7 92.25 Mbp_chr7_92246197 GAACATGAGCCGGGCTTGACCGCGAGGCGGAGGCAAGAGCCACCGCCCCCTCTTCCCCTCCCCCGAGTGAGGCGGCGCAG
CGGCCGGAGAGGGATGGGGGGCGCCCACCCAGTCTGAGCCTCG
mm4__chr2 159.16 Mbp_chr2_159160967 GCCAGGACCTGAGTGTTACCCCTCCGCCCCCTCTCCCCCTCCACGAGATGCTGGGCACAGTGTAGGGCACATGTCTGCCA
GTGAGAGC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.