CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGCGCTCGTCGTCGACAA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	(Wild-type sequence)
298.9	`TCACCATGGATGATGATATCGCCGCGCTC-----------------CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`17 bp deleted → frameshift`
296.4	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCG------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`6 bp deleted → no frameshift`
255.2	`TCACCATGGATGATGATATCGCCGCGCTCGTCG---------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`9 bp deleted → no frameshift`
219.6	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCG------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`12 bp deleted → no frameshift`
219.6	`TCACCATGGATGATGATATCGCCGCGCTCG------------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`12 bp deleted → no frameshift`
188.8	`TCACCATGGATGATGATATCGCCGCGCTCGTCG---------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`15 bp deleted → no frameshift`
180.6	`TCACCATGGATGATGATATCGCCG------------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`24 bp deleted → no frameshift`
179.6	`TCACCATGGATGATGATATCGCCGCG----------------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`16 bp deleted → frameshift`
173.1	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTC-----------CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`11 bp deleted → frameshift`
164.7	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCGACA------------TGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`12 bp deleted → no frameshift`
162.8	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCGACAA------------------GGCCGGCTTCGCGGGCGACGAT`	`18 bp deleted → no frameshift`
162.8	`TCACCATGGATGATGATATCGCCGCGCTCG------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`18 bp deleted → no frameshift`
162.8	`TCACCATGGATGATGATATCGCCG------------------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`18 bp deleted → no frameshift`
154.8	`TCACCATGGATGATGATATCGCCGC-------------------TCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`19 bp deleted → frameshift`
149.1	`TCACCATGGATGATGATATCGCCGCGCTCGTC--------------CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`14 bp deleted → frameshift`
140.0	`TCACCATGGATGATGATATCG---------------------GCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`21 bp deleted → no frameshift`
133.2	`TCACCATGGATGATGATATCGCCGCG----------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`22 bp deleted → frameshift`
133.2	`TCACCATGGATGATGATATCGC----------------------TCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`22 bp deleted → frameshift`
126.8	`TCACCATGGATGATGATATCGCCGCGC-----------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`23 bp deleted → frameshift`
114.8	`TCACCATGGATGATGATATCGCCGC-------------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`25 bp deleted → frameshift`
113.6	`TCACCATGGATGATGATATCGCCG---------------------------------------GCTTCGCGGGCGACGAT`	`39 bp deleted → no frameshift`
110.4	`TCACCATGGATGATGATATCGCCGCGCTCGTCGT--------------------GCAAGGCCGGCTTCGCGGGCGACGAT`	`20 bp deleted → frameshift`
103.6	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCG---------------------------GCTTCGCGGGCGACGAT`	`27 bp deleted → no frameshift`
103.6	`TCACCATGGATGATGATATCG---------------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`27 bp deleted → no frameshift`
99.4	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCGAC---------------------------------------GAT`	`39 bp deleted → no frameshift`
98.8	`TCACCATGGATGATGATATCGC----------------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`28 bp deleted → frameshift`
96.0	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCG---------------------------------CGGGCGACGAT`	`33 bp deleted → no frameshift`
95.1	`TCACCATGGATGATGATATCGCCGCGCTCGT-----------------------GCAAGGCCGGCTTCGCGGGCGACGAT`	`23 bp deleted → frameshift`
94.0	`TCACCATGGATGATGATATCGCCGCGC-----------------------------AAGGCCGGCTTCGCGGGCGACGAT`	`29 bp deleted → frameshift`
89.2	`TCACCATGGATGATGATATCGCCGCGCTCGTCG------------------------------GCTTCGCGGGCGACGAT`	`30 bp deleted → no frameshift`
84.8	`TCACCATGGATGATGATATCGCCGC-------------------------------AAGGCCGGCTTCGCGGGCGACGAT`	`31 bp deleted → frameshift`
84.0	`TCACCATGGATGATGATATCGCCGCG---------------------------------------------GGCGACGAT`	`45 bp deleted → no frameshift`
82.5	`TCACCATGGATGATGATATCGCCGCGCTCGTCG------------------------------------CGGGCGACGAT`	`36 bp deleted → no frameshift`
81.9	`TCACCATGGATGATGATATC--------------------------CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`26 bp deleted → frameshift`
76.8	`TCACCATGGATGATGATATCGCCGCGCTCG---------------------------------GCTTCGCGGGCGACGAT`	`33 bp deleted → no frameshift`
75.0	`TCACCATGGATGATGATATCGCCGCGCT--------------------------------------TCGCGGGCGACGAT`	`38 bp deleted → frameshift`
73.2	`TCACCATGGATGATGATATCGCCGCGC----------------------------------CGGCTTCGCGGGCGACGAT`	`34 bp deleted → frameshift`
73.2	`TCACCATGGATGATGATATCGC----------------------------------AAGGCCGGCTTCGCGGGCGACGAT`	`34 bp deleted → frameshift`
73.2	`TCACCATGG----------------------------------CTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`34 bp deleted → frameshift`
71.0	`TCACCATGGATGATGATATCGCCGCGCTCG---------------------------------------CGGGCGACGAT`	`39 bp deleted → no frameshift`
69.6	`TCACCATGGATGATGATATCGCCGCGC-------------------------------------------GGGCGACGAT`	`43 bp deleted → frameshift`
69.6	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCG-----------------------------------GGCGACGAT`	`35 bp deleted → frameshift`
66.0	`TCACCATGGATGATGATATCGCCGC------------------------------------CGGCTTCGCGGGCGACGAT`	`36 bp deleted → no frameshift`
63.7	`TCACCATGGATGATGATATCGC------------------------------------------------GGGCGACGAT`	`48 bp deleted → no frameshift`
63.0	`TCACCATG---------------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGAT`	`45 bp deleted → no frameshift`
62.8	`TCACCATGGATGATGATATCGCCGCG-------------------------------------GCTTCGCGGGCGACGAT`	`37 bp deleted → frameshift`
61.0	`TCACCATGGATGATGATATCGCCGCGCTCGTCGTCGA------------------------------------------T`	`42 bp deleted → no frameshift`
60.0	`TCACCATGGATGATGATATCGCCGCGCTCGTCG--------------------------------------GGCGACGAT`	`38 bp deleted → frameshift`
60.0	`TCACCATGGATGATG--------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGAT`	`38 bp deleted → frameshift`
54.0	`TCACCATGGATGATGATATCGCCGC----------------------------------------TTCGCGGGCGACGAT`	`40 bp deleted → frameshift`
54.0	`TCACCATGG----------------------------------------CATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`40 bp deleted → frameshift`
51.6	`TCACCATGGATGATGATATCGCCGCGCTCG-----------------------------------------GGCGACGAT`	`41 bp deleted → frameshift`
51.6	`TCACCATGGATG-----------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGAT`	`41 bp deleted → frameshift`
51.6	`TCACCATGGATGATGATATCGCCGCG-------------------------------------------------ACGAT`	`49 bp deleted → frameshift`
48.8	`TCACCATGGATGATGATATCGCCGCGCTCGTCG------------------------------------------ACGAT`	`42 bp deleted → no frameshift`
48.8	`TCACCATGGATGATGATATCG------------------------------------------GCTTCGCGGGCGACGAT`	`42 bp deleted → no frameshift`
48.8	`TCACC------------------------------------------GGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`42 bp deleted → no frameshift`
46.4	`TCACCATGGATGATGATATCGC-------------------------------------------TTCGCGGGCGACGAT`	`43 bp deleted → frameshift`
42.0	`TCACCATGGATGATGATATCGCCGCGCTCGTCG---------------------------------------------AT`	`45 bp deleted → no frameshift`
42.0	`TCACCATGGATGATGATATCGCCGCGCTCG---------------------------------------------ACGAT`	`45 bp deleted → no frameshift`
40.5	`TCACCATGGATGATG----------------------------------------CAAGGCCGGCTTCGCGGGCGACGAT`	`40 bp deleted → frameshift`
38.4	`TCACCATGGATGATGATAT---------------------------------GTGCAAGGCCGGCTTCGCGGGCGACGAT`	`33 bp deleted → no frameshift`
38.0	`TCACCATGGATGATGATATCGCCGCGC-----------------------------------------------GACGAT`	`47 bp deleted → frameshift`
38.0	`TCACCATGGATGATGATATCGCCG-----------------------------------------------GGCGACGAT`	`47 bp deleted → frameshift`
36.4	`TCACCATGGATGATGATATCGCCGCGCTCG------------------------------------------------AT`	`48 bp deleted → no frameshift`
34.8	`TCACCATGGATG-------------------------------------------CAAGGCCGGCTTCGCGGGCGACGAT`	`43 bp deleted → frameshift`
34.8	`TCACCATGGATGATGAT-----------------------------------GTGCAAGGCCGGCTTCGCGGGCGACGAT`	`35 bp deleted → frameshift`
33.3	`TC--------------------------------------------CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`44 bp deleted → frameshift`
32.8	`TCACCATGGATGATGATATCG--------------------------------------------------GGCGACGAT`	`50 bp deleted → frameshift`
31.2	`TCACCATGGATGATGATATCGCCG---------------------------------------------------ACGAT`	`51 bp deleted → no frameshift`
31.2	`TCACCATGG---------------------------------------------------CCGGCTTCGCGGGCGACGAT`	`51 bp deleted → no frameshift`
29.6	`TCACCATGGATGATGATATCGCCGCG----------------------------------------------------AT`	`52 bp deleted → frameshift`
29.6	`TCACCATGGATGATGATATCGC----------------------------------------------------GACGAT`	`52 bp deleted → frameshift`
28.5	`TCACCATG-----------------------------------------------CAAGGCCGGCTTCGCGGGCGACGAT`	`47 bp deleted → frameshift`
27.3	`TCA------------------------------------------------TGTGCAAGGCCGGCTTCGCGGGCGACGAT`	`48 bp deleted → no frameshift`
26.8	`TCACCATGGATGATGATATCGCCG------------------------------------------------------AT`	`54 bp deleted → no frameshift`
26.8	`TCACCATGGATGATGATATCG------------------------------------------------------ACGAT`	`54 bp deleted → no frameshift`
25.6	`TCACCATGG-------------------------------------------------------CTTCGCGGGCGACGAT`	`55 bp deleted → frameshift`
23.4	`TCACCA---------------------------------------------------AGGCCGGCTTCGCGGGCGACGAT`	`51 bp deleted → no frameshift`
23.2	`TCACCATGGATGATGATATCG---------------------------------------------------------AT`	`57 bp deleted → no frameshift`
23.2	`TCACC---------------------------------------------------------GGCTTCGCGGGCGACGAT`	`57 bp deleted → no frameshift`
20.1	`TCA------------------------------------------------------AGGCCGGCTTCGCGGGCGACGAT`	`54 bp deleted → no frameshift`
17.2	`TCACCATGGATGATGAT---------------------------------------------------------------`	`63 bp deleted → no frameshift`
17.2	`TCACCATGG---------------------------------------------------------------GCGACGAT`	`63 bp deleted → no frameshift`
16.4	`TCACCATGG----------------------------------------------------------------CGACGAT`	`64 bp deleted → frameshift`
15.0	`TCACCATGGATGATGA------------------------------------------------------------CGAT`	`60 bp deleted → no frameshift`
14.8	`TCACCATGGATGAT------------------------------------------------------------------`	`66 bp deleted → no frameshift`
12.9	`TCACCATGGATGA---------------------------------------------------------------CGAT`	`63 bp deleted → no frameshift`
12.8	`TCACCATGGAT---------------------------------------------------------------------`	`69 bp deleted → no frameshift`
11.1	`TCACCATGGA------------------------------------------------------------------CGAT`	`66 bp deleted → no frameshift`
11.1	`TC------------------------------------------------------------------GCGGGCGACGAT`	`66 bp deleted → no frameshift`
9.4	`TCACCATGGATGATGATAT-------------------------------------------------------------`	`61 bp deleted → frameshift`
7.8	`TCAC-------------------------------------------------------------------------GAT`	`73 bp deleted → frameshift`
5.2	`TCACCAT-------------------------------------------------------------------------`	`73 bp deleted → frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License