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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529758_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529758_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4__chr9 137.05 Mbp_chr9_137051797_F | TCGTCGGCAGCGTCACACCTTCCCTGCTTAAGCC | 59.9 | 0.44 |
| mm4__chr9 137.05 Mbp_chr9_137051797_R | GTCTCGTGGGCTCGGTTTGTCCTAGCACCTGCACC | 60.2 | 0.44 |
| mm4__chr7 5.51 Mbp_chr7_5506990_F | TCGTCGGCAGCGTCCCCTAATGTGGGCCTGAGTC | 59.8 | 0.34 |
| mm4__chr7 5.51 Mbp_chr7_5506990_R | GTCTCGTGGGCTCGGGCTCCCCTTGGTAAAGCCTT | 59.9 | 0.34 |
| mm4__chr10 92.78 Mbp_chr10_92781680_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4__chr10 92.78 Mbp_chr10_92781680_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4__chrX 13.83 Mbp_chrX_13827558_F | TCGTCGGCAGCGTCCCCTGCACTGAAAGGACACT | 59.8 | 0.32 |
| mm4__chrX 13.83 Mbp_chrX_13827558_R | GTCTCGTGGGCTCGGGAAGCTTTCCATGGTGGCAC | 59.7 | 0.32 |
| mm3__chr17 74.74 Mbp_chr17_74735751_F | TCGTCGGCAGCGTCATAGCACGCTCACCTCCAGA | 60.6 | 0.30 |
| mm3__chr17 74.74 Mbp_chr17_74735751_R | GTCTCGTGGGCTCGGCAGCCTCCAGCCCTGTATTT | 59.7 | 0.30 |
| mm4__chr11 72.66 Mbp_chr11_72655017_F | TCGTCGGCAGCGTCACCCGAGACCTGCCTCTAG | 60.0 | 0.29 |
| mm4__chr11 72.66 Mbp_chr11_72655017_R | GTCTCGTGGGCTCGGCACACCTTCCCCTGATTCCC | 60.0 | 0.29 |
| mm4__chr2 146.20 Mbp_chr2_146197292_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr2 146.20 Mbp_chr2_146197292_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chrX 28.04 Mbp_chrX_28042164_F | TCGTCGGCAGCGTCAGCAAGGGACCATGTCAGTG | 59.9 | 0.27 |
| mm4__chrX 28.04 Mbp_chrX_28042164_R | GTCTCGTGGGCTCGGTTCCTTTTCCCTTACGCCCC | 59.9 | 0.27 |
| mm4__chr15 98.41 Mbp_chr15_98410806_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr15 98.41 Mbp_chr15_98410806_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr4 148.84 Mbp_chr4_148842844_F | TCGTCGGCAGCGTCGACAACATTGGTGCTCTGCC | 59.7 | 0.23 |
| mm4__chr4 148.84 Mbp_chr4_148842844_R | GTCTCGTGGGCTCGGAAAAGCCCACAAAGCCAGGA | 60.3 | 0.23 |
| mm4__chr4 27.27 Mbp_chr4_27266794_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr4 27.27 Mbp_chr4_27266794_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr5 180.39 Mbp_chr5_180390672_F | TCGTCGGCAGCGTCCATTTCCAAGTGCTCGCCAC | 60.1 | 0.20 |
| mm4__chr5 180.39 Mbp_chr5_180390672_R | GTCTCGTGGGCTCGGGCTGGGTCCTCACCTTTCTC | 60.0 | 0.20 |
| mm4__chr1 92.23 Mbp_chr1_92229490_F | TCGTCGGCAGCGTCACCATCTTCTTTCTGTGAGCTGT | 59.9 | 0.20 |
| mm4__chr1 92.23 Mbp_chr1_92229490_R | GTCTCGTGGGCTCGGTGTTCTTGCTGCACACTGGA | 60.1 | 0.20 |
| mm4__chr10 116.56 Mbp_chr10_116555279_F | TCGTCGGCAGCGTCCCCAGCGATGCCAAATTTGT | 59.7 | 0.19 |
| mm4__chr10 116.56 Mbp_chr10_116555279_R | GTCTCGTGGGCTCGGAGACTCAGACACTATAACACCCT | 58.0 | 0.19 |
| mm4__chr14 47.07 Mbp_chr14_47074733_F | TCGTCGGCAGCGTCGCTCAGTCCAAACTCCTGCT | 59.9 | 0.19 |
| mm4__chr14 47.07 Mbp_chr14_47074733_R | GTCTCGTGGGCTCGGAGTGTAGACGGAGAGGAGACA | 59.3 | 0.19 |
| mm4__chr11 69.81 Mbp_chr11_69810980_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr11 69.81 Mbp_chr11_69810980_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr17 10.16 Mbp_chr17_10156797_F | TCGTCGGCAGCGTCTTCTTTAGAGCAGTCGGCCC | 59.7 | 0.14 |
| mm4__chr17 10.16 Mbp_chr17_10156797_R | GTCTCGTGGGCTCGGAGAGGCTTGAGCAGGGTAGA | 59.9 | 0.14 |
| mm4__chr2 86.01 Mbp_chr2_86011445_F | TCGTCGGCAGCGTCTTGTCAGTAGGGGAGGCAGA | 59.8 | 0.13 |
| mm4__chr2 86.01 Mbp_chr2_86011445_R | GTCTCGTGGGCTCGGATATGTTTCCTACCGCCCGC | 60.2 | 0.13 |
| mm4__chr3 81.66 Mbp_chr3_81663854_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 81.66 Mbp_chr3_81663854_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 145.40 Mbp_chr3_145396084_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr3 145.40 Mbp_chr3_145396084_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr10 35.33 Mbp_chr10_35333218_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr10 35.33 Mbp_chr10_35333218_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr21 45.47 Mbp_chr21_45465966_F | TCGTCGGCAGCGTCCGTGAGGGATGCAGGACATT | 60.1 | 0.10 |
| mm4__chr21 45.47 Mbp_chr21_45465966_R | GTCTCGTGGGCTCGGTCTGACACCTCCCAGGACAA | 60.1 | 0.10 |
| mm4__chr12 121.80 Mbp_chr12_121800335_F | TCGTCGGCAGCGTCCTGGAGAGCTGGGCTGGA | 60.6 | 0.08 |
| mm4__chr12 121.80 Mbp_chr12_121800335_R | GTCTCGTGGGCTCGGCCCTCTGAGAGCCGAGTCA | 60.3 | 0.08 |
| mm4__chr16 53.81 Mbp_chr16_53805647_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr16 53.81 Mbp_chr16_53805647_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr10 71.60 Mbp_chr10_71596926_F | TCGTCGGCAGCGTCGAACTGGCACTCCACAATGC | 59.7 | 0.08 |
| mm4__chr10 71.60 Mbp_chr10_71596926_R | GTCTCGTGGGCTCGGAAAGACCTGACCTCAGTGGC | 59.6 | 0.08 |
| mm3__chr1 32.20 Mbp_chr1_32199541_F | TCGTCGGCAGCGTCTCTACTCTAGCAGCTCCGGT | 59.4 | 0.07 |
| mm3__chr1 32.20 Mbp_chr1_32199541_R | GTCTCGTGGGCTCGGAGGGCTTCTTCCTGGGTACT | 59.8 | 0.07 |
| mm4__chr14 74.42 Mbp_chr14_74418561_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr14 74.42 Mbp_chr14_74418561_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr19 18.02 Mbp_chr19_18020307_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr19 18.02 Mbp_chr19_18020307_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 10.60 Mbp_chr21_10596127_F | TCGTCGGCAGCGTCTGAAGAATTGGGCAGGAGGG | 59.6 | 0.06 |
| mm4__chr21 10.60 Mbp_chr21_10596127_R | GTCTCGTGGGCTCGGGGAGCGGCATAAATAAGGAGGA | 59.9 | 0.06 |
| mm4__chr13 19.43 Mbp_chr13_19432409_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr13 19.43 Mbp_chr13_19432409_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr8 140.56 Mbp_chr8_140564041_F | TCGTCGGCAGCGTCGAGCCCTCAGAGCACTGTTG | 60.6 | 0.05 |
| mm4__chr8 140.56 Mbp_chr8_140564041_R | GTCTCGTGGGCTCGGTTTTGCAGATGGTCCTCGGG | 60.3 | 0.05 |
| mm4__chr13 24.96 Mbp_chr13_24961684_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr13 24.96 Mbp_chr13_24961684_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr13 24.59 Mbp_chr13_24587366_F | TCGTCGGCAGCGTCTGAAGAATTGGGCAGGAGGG | 59.6 | 0.05 |
| mm4__chr13 24.59 Mbp_chr13_24587366_R | GTCTCGTGGGCTCGGGGAGGGGCATAAATAAGGAGGA | 59.0 | 0.05 |
| mm4__chr17 70.07 Mbp_chr17_70071670_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr17 70.07 Mbp_chr17_70071670_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr17 49.46 Mbp_chr17_49455675_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr17 49.46 Mbp_chr17_49455675_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr11 32.44 Mbp_chr11_32436404_F | TCGTCGGCAGCGTCGTTAAGCCGGGAAAACAGCG | 60.1 | 0.04 |
| mm4__chr11 32.44 Mbp_chr11_32436404_R | GTCTCGTGGGCTCGGTCGTATCCAAACCCTCCCCT | 59.9 | 0.04 |
| mm4__chr8 25.00 Mbp_chr8_25001718_F | TCGTCGGCAGCGTCCCCTCGTTACTAAGGACGCC | 59.8 | 0.03 |
| mm4__chr8 25.00 Mbp_chr8_25001718_R | GTCTCGTGGGCTCGGTGAGAACGGCACAGATCACC | 60.0 | 0.03 |
| mm4__chr21 44.39 Mbp_chr21_44391162_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr21 44.39 Mbp_chr21_44391162_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 81.45 Mbp_chr17_81450351_F | TCGTCGGCAGCGTCCGTTCCTGGTCTCTGTGGTC | 60.0 | 0.03 |
| mm4__chr17 81.45 Mbp_chr17_81450351_R | GTCTCGTGGGCTCGGATCTTGGGGTCAGAGCATGG | 59.4 | 0.03 |
| mm2__chr14 75.05 Mbp_chr14_75051217_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm2__chr14 75.05 Mbp_chr14_75051217_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr14 70.66 Mbp_chr14_70656985_F | TCGTCGGCAGCGTCAGGGGCCATAGGTGGGATAA | 59.7 | 0.02 |
| mm4__chr14 70.66 Mbp_chr14_70656985_R | GTCTCGTGGGCTCGGGGAATCTGCCCAAGGGAGTT | 59.6 | 0.02 |
| mm3__chr11 3.70 Mbp_chr11_3700673_F | TCGTCGGCAGCGTCTGTGCTTCTGGTAAGTCTCCTG | 59.7 | 0.02 |
| mm3__chr11 3.70 Mbp_chr11_3700673_R | GTCTCGTGGGCTCGGCACAGCACTGTCCATGTGGA | 60.2 | 0.02 |
| mm4__chr19 56.12 Mbp_chr19_56118423_F | TCGTCGGCAGCGTCACAGTACACCCCACTAGGCA | 60.1 | 0.02 |
| mm4__chr19 56.12 Mbp_chr19_56118423_R | GTCTCGTGGGCTCGGCCCAGACCCTTGACCATCAC | 60.0 | 0.02 |
| mm4__chr5 141.88 Mbp_chr5_141877953_F | TCGTCGGCAGCGTCCCTTATCCCCGTCTCTCCGA | 60.1 | 0.01 |
| mm4__chr5 141.88 Mbp_chr5_141877953_R | GTCTCGTGGGCTCGGGTGCTGAGCAGAACCCTAGT | 59.3 | 0.01 |
| mm3__chr6 25.28 Mbp_chr6_25278955_F | TCGTCGGCAGCGTCGTGCGTAAGAGGGTCCCAAA | 59.9 | 0.01 |
| mm3__chr6 25.28 Mbp_chr6_25278955_R | GTCTCGTGGGCTCGGGTGAGCCGGGCTTTCCATT | 60.6 | 0.01 |
| mm4__chr22 46.13 Mbp_chr22_46127549_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 46.13 Mbp_chr22_46127549_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 25.34 Mbp_chr1_25337795_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 25.34 Mbp_chr1_25337795_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 25.27 Mbp_chr1_25267466_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 25.27 Mbp_chr1_25267466_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 77.11 Mbp_chr16_77109911_F | TCGTCGGCAGCGTCACTCATTAAAGTGGGCTCTTTGT | 58.2 | 0.00 |
| mm4__chr16 77.11 Mbp_chr16_77109911_R | GTCTCGTGGGCTCGGCATCATCCTCACACCTGCCA | 59.7 | 0.00 |
| mm4__chrX 153.27 Mbp_chrX_153273118_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chrX 153.27 Mbp_chrX_153273118_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chrX 153.13 Mbp_chrX_153126799_F | TCGTCGGCAGCGTCCTGGAGTAGGGGCAATGTCC | 59.8 | 0.00 |
| mm4__chrX 153.13 Mbp_chrX_153126799_R | GTCTCGTGGGCTCGGTACAGGTCAACGACAGGCAC | 59.9 | 0.00 |
| mm4__chr8 136.79 Mbp_chr8_136794269_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr8 136.79 Mbp_chr8_136794269_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 97.03 Mbp_chr7_97034219_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 97.03 Mbp_chr7_97034219_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 117.00 Mbp_chr7_116998477_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 117.00 Mbp_chr7_116998477_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 12.02 Mbp_chr6_12018366_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 12.02 Mbp_chr6_12018366_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 90.85 Mbp_chr5_90847938_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 90.85 Mbp_chr5_90847938_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 66.50 Mbp_chr5_66503169_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 66.50 Mbp_chr5_66503169_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 14.58 Mbp_chr5_14577147_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 14.58 Mbp_chr5_14577147_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 71.33 Mbp_chr3_71331012_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 71.33 Mbp_chr3_71331012_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 23.88 Mbp_chr3_23883015_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 23.88 Mbp_chr3_23883015_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 129.07 Mbp_chr3_129068759_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 129.07 Mbp_chr3_129068759_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 128.33 Mbp_chr3_128330693_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 128.33 Mbp_chr3_128330693_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 8.32 Mbp_chr21_8315051_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 8.32 Mbp_chr21_8315051_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 8.13 Mbp_chr21_8132006_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 8.13 Mbp_chr21_8132006_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 7.59 Mbp_chr21_7590517_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 7.59 Mbp_chr21_7590517_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 5.85 Mbp_chr21_5854520_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 5.85 Mbp_chr21_5854520_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 5.73 Mbp_chr21_5733936_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 5.73 Mbp_chr21_5733936_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 68.21 Mbp_chr17_68207735_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 68.21 Mbp_chr17_68207735_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 20.02 Mbp_chr17_20016343_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 20.02 Mbp_chr17_20016343_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr15 73.97 Mbp_chr15_73973626_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr15 73.97 Mbp_chr15_73973626_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| mm4__chr9 137.05 Mbp_chr9_137051797 |
ACACCTTCCCTGCTTAAGCCCCCAGAAACGCCCAGGTTGGAGACAAAGGGGCCCAGGTGCCACCCGGAGACCAAGGCTGC TGCCTGCTGAGAGGGTGTGGGTGCAGGTGCTAGGACAAA |
| mm4__chr7 5.51 Mbp_chr7_5506990 |
CCCTAATGTGGGCCTGAGTCAAAATGCCTGATGCCTTTTTGGTTTATACGATGAAGAGGGAGAAGAGCTGGACCTAATGA GACATGAAGTCCCCAGATCGGGGACAAAGGAAGGCAGAAGGCTTTACCAAGGGGAGC |
| mm4__chrX 13.83 Mbp_chrX_13827558 |
CCCTGCACTGAAAGGACACTCATCTGCCTTGACCTTCCTCCCACCCAGGTTGGAGACAAAGGCTTGTGCCAACTTGGATG AGGCCTAACCTTCAATACTGTGCCACCATGGAAAGCTTC |
| mm3__chr17 74.74 Mbp_chr17_74735751 |
ATAGCACGCTCACCTCCAGAAGAGGTAACCCAAGCTCTTTACTCTACTGGAGATCACCTCTGTCCCCACTCTGGGCGCTT CTCCCAGCTGACAGAAAATACCTCCAGCTGATGTCAGAAAATACAGGGCTGGAGGCTG |
| mm4__chr11 72.66 Mbp_chr11_72655017 |
ACCCGAGACCTGCCTCTAGGGCAGGGCGGGATGGTAGCAGCAGGCACACAGGTGCCTGGGAACTGGGAGTTTGATTCTCA GCCTCTGTAGTGCCCAGGTTGGGGACAGTGGGGGAATCAGGGGAAGGTGTG |
| mm4__chrX 28.04 Mbp_chrX_28042164 |
AGCAAGGGACCATGTCAGTGGTCAGAAGGTTCCTCTGAGCGCCCACACTGGGGACAGGGGTTGGGGGTGGGGGCGTAAGG GAAAAGGAA |
| mm4__chr4 148.84 Mbp_chr4_148842844 | GACAACATTGGTGCTCTGCCCCGGTCCCCTGCAATCCCTTGTCCCCATTTTGGGGGCCCTCCTGGCTTTGTGGGCTTTT |
| mm4__chr5 180.39 Mbp_chr5_180390672 |
CATTTCCAAGTGCTCGCCACTCTGGCCACAAACCCCAGGCCAGGAGGCGGCTGCCAACCTCGCTCAGGTTGCCTTGGGGC CTCCATTATCCCCAACCTGGGCATGAAGGAGAAAGGTGAGGACCCAGC |
| mm4__chr1 92.23 Mbp_chr1_92229490 |
ACCATCTTCTTTCTGTGAGCTGTACCCTTGTCCCCATTCTGGACCTGATCCGGAAGTTTGGCTAACTTTTCTTGATTTGT GCTGTTGGTTAATCTGAAGCAGAGAAGGTCTTCCAACTCCAGTGTGCAGCAAGAACA |
| mm4__chr10 116.56 Mbp_chr10_116555279 |
CCCAGCGATGCCAAATTTGTGGATGTAATTCACACGGATGGTGCCCCCATAGTCCCCAATTTGGGTGAGTTCCTCAACCC GTCCCCCAAAGGGTGTTATAGTGTCTGAGTCT |
| mm4__chr14 47.07 Mbp_chr14_47074733 |
GCTCAGTCCAAACTCCTGCTGCATTTGCTGTATACAACCTATTCTGACCTTTTGTTTATAGCTCCCAGACCAATTATACT TCCATTTCCTTTGTCCTGAATCTGGGCACCTCTTGTCTCCTCTCCGTCTACACT |
| mm4__chr17 10.16 Mbp_chr17_10156797 |
TTCTTTAGAGCAGTCGGCCCCCTCCTGCTGCAGGCCTCCCTCCCTTTTCCCCTCTCTGGGCGTGCTTCCCCATCCTCTAC CCTGCTCAAGCCTCT |
| mm4__chr2 86.01 Mbp_chr2_86011445 |
TTGTCAGTAGGGGAGGCAGAGCAGGCAGAGGGGCTGCAAAGTAGGGAGTGAGATGTCAGACAACTGCCTTTGCTGCCTCC CCCTAGTCCCCATTCTGGGAGCCCACTGTAGAAGACACAGCGGGCGGTAGGAAACATAT |
| mm4__chr21 45.47 Mbp_chr21_45465966 |
CGTGAGGGATGCAGGACATTGGGTGTGGCCCTCGAGGACTGGCCTGTCTGGAGCCCACTCCCCCGTGGGTGAACGCGGCT CTTGGTGGAGAGCCCAGTTTGGGGAGAAGGGACGGTCTTGTCCTGGGAGGTGTCAGA |
| mm4__chr12 121.80 Mbp_chr12_121800335 |
CTGGAGAGCTGGGCTGGAGGGCTGCCCAACGGGGCGTCTGCGGCCACCCCGAGGGCCTCCCGCCCTGAGTGAGGACAAAG GGGGCGACCCCCCGCCCCGCGCTGACTCGGCTCTCAGAGGG |
| mm4__chr10 71.60 Mbp_chr10_71596926 |
GAACTGGCACTCCACAATGCCCCTTGCCCAGCCCGCCCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNAGGTTGGGGACATTGGGCCACTGAGGTCAGGTCTTT |
| mm3__chr1 32.20 Mbp_chr1_32199541 |
TCTACTCTAGCAGCTCCGGTGGGGAAGGAGAGGGATTTCTGTTGTCCCCAGTCTGGGCCCCTGGTTATTGAAAAAGTTCG GAATTACTCTTTACCCTTGTGGAGTGTTCTGAGTGTTGGAAGTACCCAGGAAGAAGCCCT |
| mm4__chr21 10.60 Mbp_chr21_10596127 |
TGAAGAATTGGGCAGGAGGGCCAATGTCCCCACTCTGGGAGGGTTTTGCTCATAGTTGTATATACAGATGATCCTAACTC ATACTTGTCTTTTTACATATCCTCACACAGGAACTCCTCCTTATTTATGCCGCTCC |
| mm4__chr8 140.56 Mbp_chr8_140564041 | GAGCCCTCAGAGCACTGTTGTGCCCGATGGCCCCAAACTGGCCGCGCCCCGAGGACCATCTGCAAAA |
| mm4__chr13 24.59 Mbp_chr13_24587366 |
TGAAGAATTGGGCAGGAGGGCCAATGTCCCCACTCTGGGAGAGTTTTGCTCATAGTTATATATACAGATGAGCCTAACTC ATACCTGTCTTTTTACATATTGTCACACAGGAACTCCTCCTTATTTATGCCCCTCC |
| mm4__chr11 32.44 Mbp_chr11_32436404 |
GTTAAGCCGGGAAAACAGCGGCTAGTTTAGGAGGGAAGAAAACCCTTGGGCAAACGCCCAAAGCCTGCGTATATTCCGGC CGGAGCATCCTGGCGCCCAGTTTGGGGAGGAGGGGTGAAGGGGAGGGTTTGGATACGA |
| mm4__chr8 25.00 Mbp_chr8_25001718 |
CCCTCGTTACTAAGGACGCCGCCGCCCGCGCGCCAGGCTTGGGGACCAGGGTGACTCTGCCCTTTCTGGGCGGAATACGG GTGAAGGCTGTCTTTGGGGATCCACAGGTGATCTGTGCCGTTCTCA |
| mm4__chr17 81.45 Mbp_chr17_81450351 |
CGTTCCTGGTCTCTGTGGTCAGCCCTCAGGGCTGGCTTCCCCTACTCAGGCCCTGAGTCCCCAACCTGGGCCCGACCCTG CATGGACGCCAGCCTCTGCTGGCCCATGCTCTGACCCCAAGAT |
| mm4__chr14 70.66 Mbp_chr14_70656985 |
AGGGGCCATAGGTGGGATAACTTCTAAGTGTTCTGCCATAGCACCCAGATTGGTGCCAAAGGGCTGAGCTGACCTTAAGC TTTGGCATTACAACTCCCTTGGGCAGATTCC |
| mm3__chr11 3.70 Mbp_chr11_3700673 |
TGTGCTTCTGGTAAGTCTCCTGATGCTTCTTTAACCCAATCTGCATAGTCATGAATGACAGCAACTCCCAGATTGGGGAC AATGAGAGGACACAGTTCATCCACATGGACAGTGCTGTG |
| mm4__chr19 56.12 Mbp_chr19_56118423 |
ACAGTACACCCCACTAGGCAGGGAGTCCAGCGTCTCCAAGCCAATGACTAACACCTAGAGGACTTCGGAACAGCCTTTGT CCCCCTAGACCCCAACCTGGGGGCGGAAGGGGGCGGTTGTGATGGTCAAGGGTCTGGG |
| mm4__chr5 141.88 Mbp_chr5_141877953 | CCTTATCCCCGTCTCTCCGAGCGCGCCCAGGTTTGGGACGCGGGGACTAGGGTTCTGCTCAGCAC |
| mm3__chr6 25.28 Mbp_chr6_25278955 |
GTGCGTAAGAGGGTCCCAAAAAGAACCAGCTTCCGTGTAGACGGGTCTGCAGAATCTGCCGTCTCCCGTCTTCTGGGTCC CCTGGCCCGCCCGGAGTGGGGACAAGGAAATGGAAAGCCCGGCTCAC |
| mm4__chr16 77.11 Mbp_chr16_77109911 | ACTCATTAAAGTGGGCTCTTTGTGTCTTTGTCCCCAAGCAGGGAGCCAGCTGCTTTGGCAGGTGTGAGGATGATG |
| mm4__chrX 153.13 Mbp_chrX_153126799 |
CTGGAGTAGGGGCAATGTCCGTGCGTGCCGCTGACCTGTTGCCGCCCAGGGCGCCCAGATTAGGTGCAATGGCGGTGCCT GTCGTTGACCTGTA |