CRISPOR

Micro-homology scoring of potential deletions

Guide: CCGTCTTCCCCTCCATCGTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	(Wild-type sequence)
325.6	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCC------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`18 bp deleted → no frameshift`
282.0	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCG-------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`7 bp deleted → frameshift`
256.2	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCC-----------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`17 bp deleted → frameshift`
253.6	`GGGCGACGATGCCCCCCGGGC-----------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`23 bp deleted → frameshift`
236.0	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA---------------GGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`15 bp deleted → no frameshift`
232.2	`GGGCGACGATGCCCCCCGGGCCGTCTTCCC-------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`19 bp deleted → frameshift`
208.8	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC-------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`13 bp deleted → frameshift`
198.8	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC--------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`14 bp deleted → frameshift`
183.0	`GGGCGACGATGCCCC----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`34 bp deleted → frameshift`
179.6	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCC----------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`16 bp deleted → frameshift`
172.2	`GGGCGACGATGCCCCCCGGGCC-------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`25 bp deleted → frameshift`
166.5	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA----------------------GGTAGGGGAGCTGGCTGGGTGGG`	`22 bp deleted → frameshift`
161.6	`GGGCGACGATGCCCCCC--------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`32 bp deleted → frameshift`
153.6	`GGGCGACGATGCCCCC---------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`33 bp deleted → no frameshift`
147.2	`GGGCGACGATGCCCCCCGGGCCGTCTTCC--------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`20 bp deleted → frameshift`
135.0	`GGGCG----------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`40 bp deleted → frameshift`
133.2	`GGGCGACGATGCCCCCCGGGCCG----------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`22 bp deleted → frameshift`
133.2	`GGGCGACGATGCCCCCCGGG----------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`22 bp deleted → frameshift`
127.2	`GGGCGACGATGCCCCCC-------------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`31 bp deleted → frameshift`
121.2	`GGGCGACGATGCCCCCCGGGC--------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGGG`	`32 bp deleted → frameshift`
120.4	`GGGCGACGATGCCCCCCGG------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`24 bp deleted → no frameshift`
116.1	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA-------------------CCAGGTAGGGGAGCTGGCTGGGTGGG`	`19 bp deleted → frameshift`
114.8	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCC-------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`25 bp deleted → frameshift`
109.2	`GGGCGACGATGCCCCCCGGGCCGTCTTCCC--------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`26 bp deleted → frameshift`
109.2	`GGGCGACGATGCCCCCCGGGCC--------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`26 bp deleted → frameshift`
105.0	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT---------------------AGGGGAGCTGGCTGGGTGGG`	`21 bp deleted → no frameshift`
104.4	`GGGCGACGATGCCC-----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`35 bp deleted → frameshift`
103.6	`GGGCGACGATGCCCCCCGGGCCGTCTTCC---------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`27 bp deleted → no frameshift`
103.6	`GGGCGACGATGCCCCCCGGGCC---------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`27 bp deleted → no frameshift`
103.6	`GGGCGACGATGCCCCCCG---------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`27 bp deleted → no frameshift`
89.2	`GGGCGACGATGCCCCCC------------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`30 bp deleted → no frameshift`
80.8	`GGGCGACGATGC--------------------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`32 bp deleted → frameshift`
76.8	`GGGCGACGATGCCCCCCGG---------------------------------CACCAGGTAGGGGAGCTGGCTGGGTGGG`	`33 bp deleted → no frameshift`
75.0	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG--------------------------------------GG`	`38 bp deleted → frameshift`
73.2	`GGGCGACGATGCCCCCCGGGCC----------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`34 bp deleted → frameshift`
66.9	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG------------------------------GCTGGGTGGG`	`30 bp deleted → no frameshift`
66.6	`GGGCGACGATGCCCCCCGGG--------------------------------------------GAGCTGGCTGGGTGGG`	`44 bp deleted → frameshift`
66.0	`GGGCGACGATGCC------------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`36 bp deleted → no frameshift`
63.0	`GGGCGACGATGCCCCCCGGG---------------------------------------------AGCTGGCTGGGTGGG`	`45 bp deleted → no frameshift`
62.8	`GGGCGACG-------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`37 bp deleted → frameshift`
56.8	`GGGCGACGATGCCCCCCGGG---------------------------------------TAGGGGAGCTGGCTGGGTGGG`	`39 bp deleted → no frameshift`
56.8	`GGGCGACGATGCCCCCC---------------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`39 bp deleted → no frameshift`
56.8	`GGG---------------------------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`39 bp deleted → no frameshift`
54.9	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG----------------------------------GGTGGG`	`34 bp deleted → frameshift`
54.0	`GGGCGACGATGCCCCCCGG----------------------------------------TAGGGGAGCTGGCTGGGTGGG`	`40 bp deleted → frameshift`
54.0	`GGGCGACGATGCCCCC----------------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`40 bp deleted → frameshift`
51.6	`GGGCGACGATGCCCC-----------------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`41 bp deleted → frameshift`
51.6	`GGGCGACGATGC-----------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGGG`	`41 bp deleted → frameshift`
51.6	`GG-----------------------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`41 bp deleted → frameshift`
51.6	`GGGC-------------------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGGG`	`49 bp deleted → frameshift`
49.5	`GGGCGACGATGCCCCCCGGGCCGT------------------------------------AGGGGAGCTGGCTGGGTGGG`	`36 bp deleted → no frameshift`
48.8	`GGGCGACGATGCCC------------------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`42 bp deleted → no frameshift`
48.8	`GGGC------------------------------------------CCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGGG`	`42 bp deleted → no frameshift`
47.1	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-------------------------------------GGCTGGGTGGG`	`37 bp deleted → frameshift`
46.8	`GGGCGACGATGCCCCCCGGGC---------------------------------------------------TGGGTGGG`	`51 bp deleted → no frameshift`
46.4	`GGGCGACGATGCCCCCCGGG-------------------------------------------GGAGCTGGCTGGGTGGG`	`43 bp deleted → frameshift`
46.4	`GGGCGACGATGCC-------------------------------------------AGGTAGGGGAGCTGGCTGGGTGGG`	`43 bp deleted → frameshift`
40.0	`GGGCGACGATGCCCCCCGG----------------------------------------------AGCTGGCTGGGTGGG`	`46 bp deleted → frameshift`
38.7	`GGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-----------------------------------------GGGTGGG`	`41 bp deleted → frameshift`
38.0	`GGGCGACGATGCCCCCCGGGC-----------------------------------------------TGGCTGGGTGGG`	`47 bp deleted → frameshift`
36.6	`GGGCGACGATGCCCCCCGGG--------------------------------------------------------TGGG`	`56 bp deleted → frameshift`
34.8	`GGGCGACGATGCCCCCCGGGCCGTCT-------------------------------------------GGCTGGGTGGG`	`43 bp deleted → frameshift`
32.8	`GG--------------------------------------------------CACCAGGTAGGGGAGCTGGCTGGGTGGG`	`50 bp deleted → frameshift`
30.0	`GGGCGACGATGCCCCCCGGG------------------------------------------------------------`	`60 bp deleted → no frameshift`
29.6	`GGGCGACGATGCCCCCCGG----------------------------------------------------CTGGGTGGG`	`52 bp deleted → frameshift`
28.5	`GGGCGACGATGCCCCCCGGGCCGTCT-----------------------------------------------GGGTGGG`	`47 bp deleted → frameshift`
28.2	`GGG-------------------------------------------------------------GAGCTGGCTGGGTGGG`	`61 bp deleted → frameshift`
27.3	`GGGCGAC------------------------------------------------CAGGTAGGGGAGCTGGCTGGGTGGG`	`48 bp deleted → no frameshift`
27.0	`GGG--------------------------------------------------------------AGCTGGCTGGGTGGG`	`62 bp deleted → frameshift`
25.6	`GGGCGACGATGCCCCCCGGG-------------------------------------------------------GTGGG`	`55 bp deleted → frameshift`
24.4	`GGGCGACGATGC--------------------------------------------------------TGGCTGGGTGGG`	`56 bp deleted → frameshift`
24.4	`GGG--------------------------------------------------------TAGGGGAGCTGGCTGGGTGGG`	`56 bp deleted → frameshift`
23.2	`GGGCGACGATGCCCCCCGG---------------------------------------------------------TGGG`	`57 bp deleted → no frameshift`
23.2	`GG---------------------------------------------------------TAGGGGAGCTGGCTGGGTGGG`	`57 bp deleted → no frameshift`
21.3	`GGGCGACGATGCCCCCCGGGCCGT-----------------------------------------------------GGG`	`53 bp deleted → frameshift`
20.8	`GGGCGACGATGCCCCCCGGG-----------------------------------------------------------G`	`59 bp deleted → frameshift`
20.0	`GGGCGACGATGC------------------------------------------------------------TGGGTGGG`	`60 bp deleted → no frameshift`
20.0	`GGG------------------------------------------------------------GGAGCTGGCTGGGTGGG`	`60 bp deleted → no frameshift`
19.8	`GGGC--------------------------------------------------------------------TGGGTGGG`	`68 bp deleted → frameshift`
18.8	`GGGCGACGATGCCCCCCGG-------------------------------------------------------------`	`61 bp deleted → frameshift`
17.4	`GGGCGACGA---------------------------------------------------------GCTGGCTGGGTGGG`	`57 bp deleted → no frameshift`
17.2	`GG---------------------------------------------------------------AGCTGGCTGGGTGGG`	`63 bp deleted → no frameshift`
16.4	`GGGC----------------------------------------------------------------TGGCTGGGTGGG`	`64 bp deleted → frameshift`
15.6	`GGGCGACGATG-----------------------------------------------------------GCTGGGTGGG`	`59 bp deleted → frameshift`
15.6	`GGG-------------------------------------------------------------------------TGGG`	`73 bp deleted → frameshift`
15.0	`GGGCGA------------------------------------------------------------GCTGGCTGGGTGGG`	`60 bp deleted → no frameshift`
12.9	`GGGCGACGATG---------------------------------------------------------------GGTGGG`	`63 bp deleted → no frameshift`
12.8	`GG---------------------------------------------------------------------CTGGGTGGG`	`69 bp deleted → no frameshift`
12.6	`GGG-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`
10.8	`GGG------------------------------------------------------------------------GTGGG`	`72 bp deleted → no frameshift`
10.5	`GGGCGACGATG-------------------------------------------------------------------GG`	`67 bp deleted → frameshift`
10.0	`GG--------------------------------------------------------------------------TGGG`	`74 bp deleted → frameshift`
8.8	`GGG----------------------------------------------------------------------------G`	`76 bp deleted → frameshift`
8.0	`GG------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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