← return to the list of all guides

Contents:

PCR primers for off-targets of GAAGCCGGCCTTGCACATGC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529594_F TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG 59.8 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529594_R GTCTCGTGGGCTCGGGCTATTCTCGCAGCTCACCA 60.1 1.00
mm1__chr1 223.86 Mbp_chr1_223864788_F TCGTCGGCAGCGTCTGACCCATGCTCACCATCAC 60.0 1.00
mm1__chr1 223.86 Mbp_chr1_223864788_R GTCTCGTGGGCTCGGCGCTCGTCATTGACAACAGC 60.1 1.00
mm1__chr5 77.78 Mbp_chr5_77784926_F TCGTCGGCAGCGTCATATCACCGCGCTCGTCATT 59.9 0.92
mm1__chr5 77.78 Mbp_chr5_77784926_R GTCTCGTGGGCTCGGAACGATGGAGGGGAAGACTG 59.0 0.92
mm3__chr5 61.83 Mbp_chr5_61832982_F TCGTCGGCAGCGTCCCATCATCATGCCCTGGTGT 60.1 0.84
mm3__chr5 61.83 Mbp_chr5_61832982_R GTCTCGTGGGCTCGGGGCTGGTCATTGACAATGGC 59.8 0.84
mm3__chr1 237.93 Mbp_chr1_237926870_F TCGTCGGCAGCGTCCTCCTCCACCAGTCACGATG 59.8 0.84
mm3__chr1 237.93 Mbp_chr1_237926870_R GTCTCGTGGGCTCGGTGGAGGGAAACACAGCTTGG 60.1 0.84
mm3__chr1 103.57 Mbp_chr1_103569592_F Primer3: not found at this Tm N.d. 0.84
mm3__chr1 103.57 Mbp_chr1_103569592_R Primer3: not found at this Tm N.d. 0.84
mm4__chr20 1.16 Mbp_chr20_1160623_F TCGTCGGCAGCGTCGCTCCTCCACCGATCACAAT 59.8 0.76
mm4__chr20 1.16 Mbp_chr20_1160623_R GTCTCGTGGGCTCGGGGGAAACATGGTTCCAGGGG 60.6 0.76
mm4__chr17 81.51 Mbp_chr17_81512291_F TCGTCGGCAGCGTCCCCGACGATGGAAGGAAACA 60.0 0.76
mm4__chr17 81.51 Mbp_chr17_81512291_R GTCTCGTGGGCTCGGCGCTGGTCATTGACAATGGC 60.1 0.76
mm4__chr3 139.49 Mbp_chr3_139493854_F TCGTCGGCAGCGTCTGCCGGTCACAATGGAAGAA 59.8 0.62
mm4__chr3 139.49 Mbp_chr3_139493854_R GTCTCGTGGGCTCGGATGGAAGGGAACATGGCTCG 60.1 0.62
mm3__chr19 9.52 Mbp_chr19_9518189_F TCGTCGGCAGCGTCGCTGTCAGCTCACCATGGAT 60.1 0.59
mm3__chr19 9.52 Mbp_chr19_9518189_R GTCTCGTGGGCTCGGCCACGATGGAGGGGAAGATG 59.8 0.59
mm3__chr3 175.98 Mbp_chr3_175978516_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.58
mm3__chr3 175.98 Mbp_chr3_175978516_R GTCTCGTGGGCTCGGGGCTTGTAGTCAGCAAAGGC 59.4 0.58
mm3__chr6 110.27 Mbp_chr6_110268509_F TCGTCGGCAGCGTCAGAAAAGCAAATGTGGAGTAGCT 58.5 0.49
mm3__chr6 110.27 Mbp_chr6_110268509_R GTCTCGTGGGCTCGGGTGTCTTGTTTGCAGCCAGG 59.9 0.49
mm4__chr20 36.38 Mbp_chr20_36379162_F TCGTCGGCAGCGTCTTCCAGAGACAGGGTGGTCA 60.1 0.49
mm4__chr20 36.38 Mbp_chr20_36379162_R GTCTCGTGGGCTCGGCAACCTTTGTTCTCGCTGCC 60.0 0.49
mm2__chr9 6.83 Mbp_chr9_6834495_F TCGTCGGCAGCGTCCCGCTACCAGCTCACCATAG 59.9 0.42
mm2__chr9 6.83 Mbp_chr9_6834495_R GTCTCGTGGGCTCGGACACGATGAAGGGGAAGACG 59.7 0.42
mm4__chr8 37.26 Mbp_chr8_37260983_F TCGTCGGCAGCGTCCTCCCTTGGCTCCACATGTG 60.6 0.39
mm4__chr8 37.26 Mbp_chr8_37260983_R GTCTCGTGGGCTCGGGGAGGACAGAGATGCAGCTC 59.8 0.39
mm4__chr2 59.22 Mbp_chr2_59216299_F TCGTCGGCAGCGTCCTGACTGAGCAGTGACCCAC 60.3 0.37
mm4__chr2 59.22 Mbp_chr2_59216299_R GTCTCGTGGGCTCGGTGGTCCAGATTCTCATAAGACCAA 59.2 0.37
mm3__chr6 31.90 Mbp_chr6_31900245_F TCGTCGGCAGCGTCGCGAGGGCACATGAAGATGA 60.4 0.34
mm3__chr6 31.90 Mbp_chr6_31900245_R GTCTCGTGGGCTCGGGCTTCTCAGGTGGAAACCCC 60.6 0.34
mm4__chr20 57.01 Mbp_chr20_57011628_F TCGTCGGCAGCGTCAAGGTAACAGGGGTGCGATG 60.0 0.31
mm4__chr20 57.01 Mbp_chr20_57011628_R GTCTCGTGGGCTCGGGAAGGGCATGGAGGGAACAT 59.7 0.31
mm4__chr3 103.87 Mbp_chr3_103874331_F TCGTCGGCAGCGTCTCAGGGTCAGGTTGGTGGTA 60.1 0.30
mm4__chr3 103.87 Mbp_chr3_103874331_R GTCTCGTGGGCTCGGGGGAAGCATACTGACCCCAG 59.8 0.30
mm4__chr3 103.87 Mbp_chr3_103874097_F Primer3: not found at this Tm N.d. 0.30
mm4__chr3 103.87 Mbp_chr3_103874097_R Primer3: not found at this Tm N.d. 0.30
mm3__chr9 123.11 Mbp_chr9_123110951_F TCGTCGGCAGCGTCAAAGCAGTGCTCAGTACCCC 59.9 0.29
mm3__chr9 123.11 Mbp_chr9_123110951_R GTCTCGTGGGCTCGGTGCCATTAGACGCATCCCTC 59.8 0.29
mm4__chr19 49.00 Mbp_chr19_48998256_F Primer3: not found at this Tm N.d. 0.27
mm4__chr19 49.00 Mbp_chr19_48998256_R Primer3: not found at this Tm N.d. 0.27
mm4__chr16 84.25 Mbp_chr16_84252118_F TCGTCGGCAGCGTCTCACATAGAAAGCCGTGGGG 59.7 0.27
mm4__chr16 84.25 Mbp_chr16_84252118_R GTCTCGTGGGCTCGGTGTGCTCCGGACTCCAAATC 60.0 0.27
mm0__chr5 98.34 Mbp_chr5_98338783_F TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT 60.0 0.25
mm0__chr5 98.34 Mbp_chr5_98338783_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.25
mm0__chr22 41.08 Mbp_chr22_41075176_F TCGTCGGCAGCGTCATGCCCACCATCATGACCTG 60.1 0.25
mm0__chr22 41.08 Mbp_chr22_41075176_R GTCTCGTGGGCTCGGTATTGCTGCGCTCGTTGTTG 59.8 0.25
mm4__chr15 74.11 Mbp_chr15_74105722_F Primer3: not found at this Tm N.d. 0.25
mm4__chr15 74.11 Mbp_chr15_74105722_R Primer3: not found at this Tm N.d. 0.25
mm4__chr12 57.18 Mbp_chr12_57181237_F TCGTCGGCAGCGTCTGTCAACAACGGTGACTGCT 60.1 0.24
mm4__chr12 57.18 Mbp_chr12_57181237_R GTCTCGTGGGCTCGGACAAGGCTGGGAGGAAAGC 59.9 0.24
mm1__chr11 1.80 Mbp_chr11_1804032_F TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG 59.8 0.24
mm1__chr11 1.80 Mbp_chr11_1804032_R GTCTCGTGGGCTCGGCCCAGCTCACCATGGATGAT 59.5 0.24
mm4__chrX 8.02 Mbp_chrX_8017680_F TCGTCGGCAGCGTCTGTACAATGGGATGGCGACC 60.1 0.23
mm4__chrX 8.02 Mbp_chrX_8017680_R GTCTCGTGGGCTCGGTCCAGGGGCAAAACTGCATA 59.5 0.23
mm4__chr22 30.64 Mbp_chr22_30636692_F Primer3: not found at this Tm N.d. 0.23
mm4__chr22 30.64 Mbp_chr22_30636692_R Primer3: not found at this Tm N.d. 0.23
mm1__chr22 15.72 Mbp_chr22_15721526_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr22 15.72 Mbp_chr22_15721526_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.23
mm1__chr2 131.63 Mbp_chr2_131626178_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 131.63 Mbp_chr2_131626178_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.23
mm1__chr2 131.26 Mbp_chr2_131263595_F TCGTCGGCAGCGTCTGGATGATGATACCGCCGTG 59.9 0.23
mm1__chr2 131.26 Mbp_chr2_131263595_R GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC 60.1 0.23
mm1__chr2 130.66 Mbp_chr2_130656789_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 130.66 Mbp_chr2_130656789_R GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC 60.1 0.23
mm1__chr2 130.46 Mbp_chr2_130463880_F TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC 60.1 0.23
mm1__chr2 130.46 Mbp_chr2_130463880_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 130.08 Mbp_chr2_130075308_F TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC 60.1 0.23
mm1__chr2 130.08 Mbp_chr2_130075308_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.40 Mbp_chr14_19402845_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.23
mm1__chr14 19.40 Mbp_chr14_19402845_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.00 Mbp_chr14_18998907_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.00 Mbp_chr14_18998907_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.23
mm4__chr5 176.79 Mbp_chr5_176792600_F TCGTCGGCAGCGTCAGGTGCTCAAGTGTTCCCTG 59.8 0.22
mm4__chr5 176.79 Mbp_chr5_176792600_R GTCTCGTGGGCTCGGCTGCCTTCTCCTTTTGCCCT 60.2 0.22
mm3__chr16 66.37 Mbp_chr16_66371351_F TCGTCGGCAGCGTCCACTCCAGCAGGCACACC 60.6 0.22
mm3__chr16 66.37 Mbp_chr16_66371351_R GTCTCGTGGGCTCGGCACCCGCTTTCAGCTAGGAT 59.8 0.22
mm3__chrY 18.15 Mbp_chrY_18147923_F Primer3: not found at this Tm N.d. 0.22
mm3__chrY 18.15 Mbp_chrY_18147923_R Primer3: not found at this Tm N.d. 0.22
mm3__chrY 17.76 Mbp_chrY_17758062_F TCGTCGGCAGCGTCAACGGTGGAGGGAAACATGG 60.2 0.22
mm3__chrY 17.76 Mbp_chrY_17758062_R GTCTCGTGGGCTCGGTCCTCTGTCCCACTCCTCTG 59.9 0.22
mm3__chrX 53.14 Mbp_chrX_53143850_F TCGTCGGCAGCGTCGATGGAGGGGAACACAGCTC 60.1 0.22
mm3__chrX 53.14 Mbp_chrX_53143850_R GTCTCGTGGGCTCGGTGCTCTTCCACAGGTTGCAA 60.1 0.22
mm3__chr5 15.45 Mbp_chr5_15450739_F Primer3: not found at this Tm N.d. 0.22
mm3__chr5 15.45 Mbp_chr5_15450739_R Primer3: not found at this Tm N.d. 0.22
mm3__chr2 57.76 Mbp_chr2_57764992_F TCGTCGGCAGCGTCAGATTGCTGGCTCGTCAATGA 60.0 0.22
mm3__chr2 57.76 Mbp_chr2_57764992_R GTCTCGTGGGCTCGGCTTCTTGCCCATGCCCACTA 60.0 0.22
mm2__chr15 43.99 Mbp_chr15_43990121_F TCGTCGGCAGCGTCAATGAAGGGGAAGATGGCCC 59.7 0.21
mm2__chr15 43.99 Mbp_chr15_43990121_R GTCTCGTGGGCTCGGGCCAGCTCACCATGGATGAT 60.1 0.21
mm4__chr2 21.06 Mbp_chr2_21057837_F TCGTCGGCAGCGTCTCCTGTTAGCCAGACTTGGT 58.2 0.21
mm4__chr2 21.06 Mbp_chr2_21057837_R GTCTCGTGGGCTCGGTGGGTCTGTGTAGGATCCACT 59.9 0.21
mm3__chr22 39.97 Mbp_chr22_39971668_F TCGTCGGCAGCGTCCAGGACGGGGTTGGAGAGA 60.6 0.20
mm3__chr22 39.97 Mbp_chr22_39971668_R GTCTCGTGGGCTCGGTCCCAGTACTTCGCTCCTCA 59.9 0.20
mm4__chr5 66.28 Mbp_chr5_66283509_F Primer3: not found at this Tm N.d. 0.19
mm4__chr5 66.28 Mbp_chr5_66283509_R Primer3: not found at this Tm N.d. 0.19
mm4__chr6 166.24 Mbp_chr6_166237177_F TCGTCGGCAGCGTCTTCTCAGGTACCAGCACCCA 60.4 0.18
mm4__chr6 166.24 Mbp_chr6_166237177_R GTCTCGTGGGCTCGGTCCTCCTTCACCTCCTCAGG 59.9 0.18
mm4__chrX 153.97 Mbp_chrX_153966444_F TCGTCGGCAGCGTCATTCAGCTGAGTCACTGGGC 60.0 0.18
mm4__chrX 153.97 Mbp_chrX_153966444_R GTCTCGTGGGCTCGGATGAGAAAGAGGCAGAGGGC 59.4 0.18
mm4__chr21 45.12 Mbp_chr21_45121863_F TCGTCGGCAGCGTCAGCCGTTGTTTTCTGTACGTA 57.9 0.18
mm4__chr21 45.12 Mbp_chr21_45121863_R GTCTCGTGGGCTCGGAGAGGAGGGGATACAGCTGG 60.1 0.18
mm2__chr18 62.44 Mbp_chr18_62442048_F TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT 60.1 0.17
mm2__chr18 62.44 Mbp_chr18_62442048_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.17
mm4__chr5 14.36 Mbp_chr5_14355139_F TCGTCGGCAGCGTCTTGCTTTGGGAGTGAGTGGG 60.1 0.15
mm4__chr5 14.36 Mbp_chr5_14355139_R GTCTCGTGGGCTCGGACATCCATGACATTATCCAATCCA 58.0 0.15
mm2__chrX 46.29 Mbp_chrX_46289065_F TCGTCGGCAGCGTCTGGAGGGGAAGACAACTGGG 61.1 0.15
mm2__chrX 46.29 Mbp_chrX_46289065_R GTCTCGTGGGCTCGGCTCCATCACCAGTCCACACC 60.0 0.15
mm4__chr6 9.20 Mbp_chr6_9202383_F TCGTCGGCAGCGTCTGAAAGCCCTGATGTTGCCA 60.1 0.15
mm4__chr6 9.20 Mbp_chr6_9202383_R GTCTCGTGGGCTCGGGATGAAACCACGTCAAAATACAAAA 57.3 0.15
mm4__chr17 77.10 Mbp_chr17_77095018_F TCGTCGGCAGCGTCTCGTTCTCCTGTGTGGTGTC 59.6 0.14
mm4__chr17 77.10 Mbp_chr17_77095018_R GTCTCGTGGGCTCGGCCTCGAGGAACTGTGCACAT 60.0 0.14
mm4__chr19 1.47 Mbp_chr19_1471777_F TCGTCGGCAGCGTCTTTGAGGGTTGGGTGGATGG 59.8 0.13
mm4__chr19 1.47 Mbp_chr19_1471777_R GTCTCGTGGGCTCGGGGACAGCTCCTTCTGCCAG 60.0 0.13
mm4__chr6 150.68 Mbp_chr6_150682690_F Primer3: not found at this Tm N.d. 0.13
mm4__chr6 150.68 Mbp_chr6_150682690_R Primer3: not found at this Tm N.d. 0.13
mm4__chrUn_KI270741v1 141.53 Kbp_chrUn_KI270741v1_141532_F TCGTCGGCAGCGTCGGAGAAGCTGGAAAGCGACT 60.0 0.11
mm4__chrUn_KI270741v1 141.53 Kbp_chrUn_KI270741v1_141532_R GTCTCGTGGGCTCGGGAGTTCTTCCAGCTGCGTGA 60.3 0.11
mm4__chrUn_GL000219v1 22.62 Kbp_chrUn_GL000219v1_22623_F TCGTCGGCAGCGTCGGAGAAGCTGGAAAGCGACT 60.0 0.11
mm4__chrUn_GL000219v1 22.62 Kbp_chrUn_GL000219v1_22623_R GTCTCGTGGGCTCGGGAGTTCTTCCAGCTGCGTGA 60.3 0.11
mm4__chr13 113.43 Mbp_chr13_113433580_F TCGTCGGCAGCGTCCCCACAAGATTTCCAGCCCA 60.2 0.10
mm4__chr13 113.43 Mbp_chr13_113433580_R GTCTCGTGGGCTCGGGCCATCTGCACGTCTGCTTA 60.7 0.10
mm4__chr2 75.17 Mbp_chr2_75172685_F TCGTCGGCAGCGTCCCCCAGGACAAGAGGCTCTT 61.2 0.10
mm4__chr2 75.17 Mbp_chr2_75172685_R GTCTCGTGGGCTCGGGAATGCAAGTACTTTCTGCTGT 57.2 0.10
mm4__chr16 81.59 Mbp_chr16_81589548_F TCGTCGGCAGCGTCTATGGCGAGTGCTTATGGGC 60.2 0.10
mm4__chr16 81.59 Mbp_chr16_81589548_R GTCTCGTGGGCTCGGATCGTCCCACAGAGAGACCA 59.9 0.10
mm3__chr11 1.20 Mbp_chr11_1199701_F TCGTCGGCAGCGTCCCCATCCCTCACTTCTGTGG 59.7 0.09
mm3__chr11 1.20 Mbp_chr11_1199701_R GTCTCGTGGGCTCGGCACGGTTCTGGTACGGGG 59.7 0.09
mm4__chr17 46.95 Mbp_chr17_46952273_F TCGTCGGCAGCGTCTGCTGGTGCCATTGTCTCTC 60.3 0.09
mm4__chr17 46.95 Mbp_chr17_46952273_R GTCTCGTGGGCTCGGCAAACCGACTGCTTTGGGC 60.0 0.09
mm4__chr5 141.82 Mbp_chr5_141822764_F TCGTCGGCAGCGTCTCTCCTTCACCACCTCCATCT 59.9 0.09
mm4__chr5 141.82 Mbp_chr5_141822764_R GTCTCGTGGGCTCGGTGGGGCTTACAGTCAAACCA 59.1 0.09
mm4__chr10 66.31 Mbp_chr10_66305038_F Primer3: not found at this Tm N.d. 0.08
mm4__chr10 66.31 Mbp_chr10_66305038_R Primer3: not found at this Tm N.d. 0.08
mm4__chr19 5.81 Mbp_chr19_5807475_F TCGTCGGCAGCGTCTCGGGAGATGGACAGGAGG 60.0 0.08
mm4__chr19 5.81 Mbp_chr19_5807475_R GTCTCGTGGGCTCGGCATCTCCGAGACACCCTTCC 59.5 0.08
mm4__chr2 101.00 Mbp_chr2_101001441_F Primer3: not found at this Tm N.d. 0.08
mm4__chr2 101.00 Mbp_chr2_101001441_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 48.07 Mbp_chr1_48070084_F TCGTCGGCAGCGTCATTCCTGGCCAAGCATCCAT 59.7 0.08
mm4__chr1 48.07 Mbp_chr1_48070084_R GTCTCGTGGGCTCGGCTGTCACCTGGAACCTAGCC 59.7 0.08
mm4__chr5 172.98 Mbp_chr5_172983758_F TCGTCGGCAGCGTCGAGACAGCCAGGCGAAGG 60.1 0.08
mm4__chr5 172.98 Mbp_chr5_172983758_R GTCTCGTGGGCTCGGTCCCACCAGGAAGTGTCAAT 58.5 0.08
mm4__chr7 1.25 Mbp_chr7_1248874_F Primer3: not found at this Tm N.d. 0.07
mm4__chr7 1.25 Mbp_chr7_1248874_R Primer3: not found at this Tm N.d. 0.07
mm4__chr8 49.30 Mbp_chr8_49295643_F Primer3: not found at this Tm N.d. 0.07
mm4__chr8 49.30 Mbp_chr8_49295643_R Primer3: not found at this Tm N.d. 0.07
mm4__chr19 28.86 Mbp_chr19_28863407_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 28.86 Mbp_chr19_28863407_R Primer3: not found at this Tm N.d. 0.06
mm4__chr15 65.98 Mbp_chr15_65982381_F TCGTCGGCAGCGTCCATCCCTTGCCGTGGGTG 60.7 0.06
mm4__chr15 65.98 Mbp_chr15_65982381_R GTCTCGTGGGCTCGGTGTGTAACCCCATCACAGGC 59.9 0.06
mm4__chr17 21.36 Mbp_chr17_21364077_F TCGTCGGCAGCGTCCTCCAGACAAGGGCAGTGG 60.0 0.06
mm4__chr17 21.36 Mbp_chr17_21364077_R GTCTCGTGGGCTCGGAGCTTCCTGTGAGCCAGTTC 59.9 0.06
mm4__chr1 228.86 Mbp_chr1_228863148_F TCGTCGGCAGCGTCTCAGAGAGACATGGGACTACGA 59.7 0.06
mm4__chr1 228.86 Mbp_chr1_228863148_R GTCTCGTGGGCTCGGTCTTTGTGTCTCTGGTGCCC 59.8 0.06
mm4__chr13 38.68 Mbp_chr13_38679190_F TCGTCGGCAGCGTCTGGGGAACTGGCTAAGCTTC 59.6 0.05
mm4__chr13 38.68 Mbp_chr13_38679190_R GTCTCGTGGGCTCGGTTTCCAGCCCTCTCCTAGAA 57.3 0.05
mm4__chr22 45.31 Mbp_chr22_45310735_F TCGTCGGCAGCGTCCGACCACCTCCTAAGCTGTG 60.1 0.05
mm4__chr22 45.31 Mbp_chr22_45310735_R GTCTCGTGGGCTCGGCCACCTGAGTCTCAGCAGAA 59.0 0.05
mm4__chr4 157.00 Mbp_chr4_156995146_F Primer3: not found at this Tm N.d. 0.05
mm4__chr4 157.00 Mbp_chr4_156995146_R Primer3: not found at this Tm N.d. 0.05
mm4__chr16 69.74 Mbp_chr16_69742673_F TCGTCGGCAGCGTCGAAGAAGGGGAGCCACAGTC 60.0 0.05
mm4__chr16 69.74 Mbp_chr16_69742673_R GTCTCGTGGGCTCGGAGGCAGTCAATTACCACCCG 60.0 0.05
mm4__chr8 117.65 Mbp_chr8_117650091_F TCGTCGGCAGCGTCAGCTACTTGTCCCAAACGGG 59.9 0.05
mm4__chr8 117.65 Mbp_chr8_117650091_R GTCTCGTGGGCTCGGTTCACCATGGTGCCTTGGAT 59.5 0.05
mm4__chr22 24.54 Mbp_chr22_24543240_F TCGTCGGCAGCGTCCAACTCAAGTCCTCCACCCC 59.9 0.05
mm4__chr22 24.54 Mbp_chr22_24543240_R GTCTCGTGGGCTCGGTCTGGGCCTTTCTCCAACTG 59.5 0.05
mm4__chr10 119.98 Mbp_chr10_119975614_F Primer3: not found at this Tm N.d. 0.05
mm4__chr10 119.98 Mbp_chr10_119975614_R Primer3: not found at this Tm N.d. 0.05
mm2__chr2 73.90 Mbp_chr2_73901354_F TCGTCGGCAGCGTCGTGTGAAGAGGAGACCACCG 60.0 0.04
mm2__chr2 73.90 Mbp_chr2_73901354_R GTCTCGTGGGCTCGGCCCACAATGGAGGGGAAGAC 60.0 0.04
mm4__chr7 156.95 Mbp_chr7_156948914_F TCGTCGGCAGCGTCAGGAACCTCAAACGCTCTGC 60.6 0.04
mm4__chr7 156.95 Mbp_chr7_156948914_R GTCTCGTGGGCTCGGTGGATACGGACCAGGGAGAC 60.3 0.04
mm4__chr10 132.27 Mbp_chr10_132270381_F Primer3: not found at this Tm N.d. 0.04
mm4__chr10 132.27 Mbp_chr10_132270381_R Primer3: not found at this Tm N.d. 0.04
mm2__chr19 5.50 Mbp_chr19_5496885_F TCGTCGGCAGCGTCCACGGCCCTTTGGTAACTCT 59.9 0.04
mm2__chr19 5.50 Mbp_chr19_5496885_R GTCTCGTGGGCTCGGGACCTGGGCAGTTGGAAACA 60.4 0.04
mm4__chr13 110.21 Mbp_chr13_110208106_F TCGTCGGCAGCGTCTGGGCGCTCTGGTTGTAATT 59.9 0.04
mm4__chr13 110.21 Mbp_chr13_110208106_R GTCTCGTGGGCTCGGAAGAAGGGATGCCTGCCATT 59.6 0.04
mm4__chr1 53.37 Mbp_chr1_53371273_F TCGTCGGCAGCGTCATTAACAGGCTCATCGGGGC 60.1 0.04
mm4__chr1 53.37 Mbp_chr1_53371273_R GTCTCGTGGGCTCGGTGAGATGCAACTCCAGGCTG 60.0 0.04
mm4__chr19 2.19 Mbp_chr19_2192888_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 2.19 Mbp_chr19_2192888_R Primer3: not found at this Tm N.d. 0.04
mm4__chr16 1.73 Mbp_chr16_1728981_F TCGTCGGCAGCGTCCTGAGTGTTCTCCCATCGCA 59.7 0.04
mm4__chr16 1.73 Mbp_chr16_1728981_R GTCTCGTGGGCTCGGTGTAACCACAGAGGGAGGGG 60.5 0.04
mm4__chr21 31.87 Mbp_chr21_31866258_F TCGTCGGCAGCGTCGGAAAGCAGAGCCGAGTTGA 60.3 0.04
mm4__chr21 31.87 Mbp_chr21_31866258_R GTCTCGTGGGCTCGGTCACCAGTCACAGAACTGGG 59.2 0.04
mm4__chr1 115.48 Mbp_chr1_115479608_F TCGTCGGCAGCGTCTTTTCCAGTTGCGAGTCCCA 59.8 0.04
mm4__chr1 115.48 Mbp_chr1_115479608_R GTCTCGTGGGCTCGGTGTGGAGGTCCCTCAAAGGT 60.4 0.04
mm4__chr6 40.94 Mbp_chr6_40935908_F Primer3: not found at this Tm N.d. 0.04
mm4__chr6 40.94 Mbp_chr6_40935908_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.70 Mbp_chr18_79695954_F TCGTCGGCAGCGTCGAGACTCAGCTGAAAGCCCT 59.3 0.04
mm4__chr18 79.70 Mbp_chr18_79695954_R GTCTCGTGGGCTCGGCCTGCTGCTTCCCGTTCTTA 60.0 0.04
mm4__chr1 31.70 Mbp_chr1_31701077_F TCGTCGGCAGCGTCGCAGAGGGATAGGTGGGAGA 60.1 0.03
mm4__chr1 31.70 Mbp_chr1_31701077_R GTCTCGTGGGCTCGGAGAGTGCAGTGGGGTCATTG 59.9 0.03
mm4__chr18 44.89 Mbp_chr18_44891188_F Primer3: not found at this Tm N.d. 0.03
mm4__chr18 44.89 Mbp_chr18_44891188_R Primer3: not found at this Tm N.d. 0.03
mm4__chr7 391.85 Kbp_chr7_391849_F TCGTCGGCAGCGTCACAGAGATCTGAGAGGGGCT 59.3 0.03
mm4__chr7 391.85 Kbp_chr7_391849_R GTCTCGTGGGCTCGGCCCTCCCTGTCATCATGCAG 60.1 0.03
mm3__chr10 69.02 Mbp_chr10_69022793_F TCGTCGGCAGCGTCCCAGGGTGATATCACTGGGC 59.8 0.03
mm3__chr10 69.02 Mbp_chr10_69022793_R GTCTCGTGGGCTCGGCAAGATGGAGGGGAAGGCG 60.1 0.03
mm4__chr2 91.73 Mbp_chr2_91734281_F Primer3: not found at this Tm N.d. 0.03
mm4__chr2 91.73 Mbp_chr2_91734281_R Primer3: not found at this Tm N.d. 0.03
mm4__chr2 89.66 Mbp_chr2_89660143_F Primer3: not found at this Tm N.d. 0.03
mm4__chr2 89.66 Mbp_chr2_89660143_R Primer3: not found at this Tm N.d. 0.03
mm4__chr13 18.31 Mbp_chr13_18312372_F Primer3: not found at this Tm N.d. 0.03
mm4__chr13 18.31 Mbp_chr13_18312372_R Primer3: not found at this Tm N.d. 0.03
mm4__chr10 131.82 Mbp_chr10_131820966_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 131.82 Mbp_chr10_131820966_R Primer3: not found at this Tm N.d. 0.03
mm4__chr19 8.13 Mbp_chr19_8133014_F TCGTCGGCAGCGTCCAGGCTCACCCATGCAGTAG 60.4 0.03
mm4__chr19 8.13 Mbp_chr19_8133014_R GTCTCGTGGGCTCGGGTGTCTCAACGAGGATGGCA 60.0 0.03
mm4__chr19 41.91 Mbp_chr19_41912922_F TCGTCGGCAGCGTCAGACGCAGCTACAGTCCATG 59.8 0.02
mm4__chr19 41.91 Mbp_chr19_41912922_R GTCTCGTGGGCTCGGGGCTGACCTGTGTCTGTCTC 60.0 0.02
mm4__chr2 105.60 Mbp_chr2_105597301_F TCGTCGGCAGCGTCAGGCCACCTCTCCCTGATAG 60.1 0.02
mm4__chr2 105.60 Mbp_chr2_105597301_R GTCTCGTGGGCTCGGCTACTGTGTCACCAGCTGGG 60.0 0.02
mm2__chr16 26.87 Mbp_chr16_26867874_F Primer3: not found at this Tm N.d. 0.02
mm2__chr16 26.87 Mbp_chr16_26867874_R Primer3: not found at this Tm N.d. 0.02
mm4__chr10 62.66 Mbp_chr10_62657540_F Primer3: not found at this Tm N.d. 0.02
mm4__chr10 62.66 Mbp_chr10_62657540_R Primer3: not found at this Tm N.d. 0.02
mm4__chr18 79.77 Mbp_chr18_79771771_F TCGTCGGCAGCGTCTTCCCAGAGCAAGAAAGCGT 59.8 0.02
mm4__chr18 79.77 Mbp_chr18_79771771_R GTCTCGTGGGCTCGGTTCCGTGTTCTCTTCCGGAC 59.6 0.02
mm4__chr22 30.71 Mbp_chr22_30712836_F TCGTCGGCAGCGTCACAGGTTGTATCTTGGGGTCA 58.6 0.02
mm4__chr22 30.71 Mbp_chr22_30712836_R GTCTCGTGGGCTCGGACCAGGGCTCCTTCCTTTAC 59.0 0.02
mm4__chr1 210.58 Mbp_chr1_210583310_F TCGTCGGCAGCGTCTCTGTAAGCAGTGACCACGC 60.3 0.01
mm4__chr1 210.58 Mbp_chr1_210583310_R GTCTCGTGGGCTCGGATCAGCACCACCTTCTCAGC 60.0 0.01
mm4__chrX 153.37 Mbp_chrX_153371479_F Primer3: not found at this Tm N.d. 0.01
mm4__chrX 153.37 Mbp_chrX_153371479_R Primer3: not found at this Tm N.d. 0.01
mm4__chr16 3.67 Mbp_chr16_3671584_F TCGTCGGCAGCGTCGACCACAAGCTTCAGAGGCC 60.9 0.01
mm4__chr16 3.67 Mbp_chr16_3671584_R GTCTCGTGGGCTCGGCTACCTGCTCCTTTGCCCC 60.0 0.01
mm4__chr1 162.45 Mbp_chr1_162445219_F TCGTCGGCAGCGTCCTCCAGTTCTGTGGGGCAG 60.0 0.01
mm4__chr1 162.45 Mbp_chr1_162445219_R GTCTCGTGGGCTCGGATGGAGACCACTGGGGAAGT 60.1 0.01
mm4__chr19 48.49 Mbp_chr19_48490784_F TCGTCGGCAGCGTCAACGGAGGTGGGATCAGGAT 60.3 0.01
mm4__chr19 48.49 Mbp_chr19_48490784_R GTCTCGTGGGCTCGGCCCGACTCTTGCCCATCTTT 60.0 0.01
mm4__chr5 179.12 Mbp_chr5_179124142_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 179.12 Mbp_chr5_179124142_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 67.39 Mbp_chr12_67390342_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 67.39 Mbp_chr12_67390342_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 92.11 Mbp_chr9_92105021_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 92.11 Mbp_chr9_92105021_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 87.86 Mbp_chr9_87858440_F TCGTCGGCAGCGTCTCAGCAAACCTGGAGAGCTG 59.9 0.00
mm4__chr9 87.86 Mbp_chr9_87858440_R GTCTCGTGGGCTCGGAGTTCCAACAGTGCTCCCTG 59.8 0.00
mm4__chr9 82.43 Mbp_chr9_82432288_F TCGTCGGCAGCGTCCCCACCATCACCATCAGCTG 60.7 0.00
mm4__chr9 82.43 Mbp_chr9_82432288_R GTCTCGTGGGCTCGGGAAGCCTGACCCACTTCCAA 59.8 0.00
mm4__chr9 28.15 Mbp_chr9_28148806_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 28.15 Mbp_chr9_28148806_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 27.01 Mbp_chr8_27011527_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 27.01 Mbp_chr8_27011527_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 63.23 Mbp_chr6_63229492_F TCGTCGGCAGCGTCAGCATCAGCAAGCATGGAGA 59.7 0.00
mm4__chr6 63.23 Mbp_chr6_63229492_R GTCTCGTGGGCTCGGGAAGCCTGACCCACTTCCAA 59.8 0.00
mm4__chr2 68.53 Mbp_chr2_68528620_F TCGTCGGCAGCGTCGCTGGGCAGGTGGTCTTTAC 60.9 0.00
mm4__chr2 68.53 Mbp_chr2_68528620_R GTCTCGTGGGCTCGGCACTTCCAACAGTGCTCCCT 59.8 0.00
mm4__chr13 77.66 Mbp_chr13_77661782_F TCGTCGGCAGCGTCAGAGCTGGGCAGGTAGTCTT 60.2 0.00
mm4__chr13 77.66 Mbp_chr13_77661782_R GTCTCGTGGGCTCGGTCACTTCCAACAGTGCTCCC 59.8 0.00
mm4__chr10 31.36 Mbp_chr10_31363205_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 31.36 Mbp_chr10_31363205_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 152.08 Mbp_chr1_152079963_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 152.08 Mbp_chr1_152079963_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 134.55 Mbp_chr9_134551454_F TCGTCGGCAGCGTCCATGACCTGGCATCCTCCG 60.2 0.00
mm4__chr9 134.55 Mbp_chr9_134551454_R GTCTCGTGGGCTCGGCCTGGACCGTTCAACCAGC 60.9 0.00
mm4__chr9 127.93 Mbp_chr9_127928072_F TCGTCGGCAGCGTCCCACTGGGAATTCTGCCCTC 60.3 0.00
mm4__chr9 127.93 Mbp_chr9_127928072_R GTCTCGTGGGCTCGGCCTACCTGCAGTTCCTCAGC 60.1 0.00
mm4__chr8 56.54 Mbp_chr8_56540406_F TCGTCGGCAGCGTCCACTTACCTGCCTGCCAGAC 60.6 0.00
mm4__chr8 56.54 Mbp_chr8_56540406_R GTCTCGTGGGCTCGGCTGACTAGCATGTGGGCTCC 60.1 0.00
mm4__chr3 122.25 Mbp_chr3_122248075_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 122.25 Mbp_chr3_122248075_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 46.53 Mbp_chr18_46528931_F TCGTCGGCAGCGTCATCCAGCCAACAACCTCGAG 60.0 0.00
mm4__chr18 46.53 Mbp_chr18_46528931_R GTCTCGTGGGCTCGGCTCCTAGCTGATGCACCCAC 60.1 0.00
mm4__chr15 77.57 Mbp_chr15_77572054_F TCGTCGGCAGCGTCATCGTGGTGTCACTTGGCAT 59.9 0.00
mm4__chr15 77.57 Mbp_chr15_77572054_R GTCTCGTGGGCTCGGTTCTCCTTGCCCTTTGACCC 59.8 0.00
mm4__chr11 7.57 Mbp_chr11_7565658_F TCGTCGGCAGCGTCCTACATGAACCCCTTCCCGG 59.8 0.00
mm4__chr11 7.57 Mbp_chr11_7565658_R GTCTCGTGGGCTCGGTGCTCTCTCCTGAGGAAGCT 59.9 0.00
mm4__chr10 130.41 Mbp_chr10_130409532_F TCGTCGGCAGCGTCAGTGATGTGTGCATGGCTGT 60.2 0.00
mm4__chr10 130.41 Mbp_chr10_130409532_R GTCTCGTGGGCTCGGTCCTTGGTCCATGCCTTGTT 59.5 0.00
mm3__chr6 137.00 Mbp_chr6_136997175_F TCGTCGGCAGCGTCGGCTGGAGGAACATCAGTCC 60.1 0.00
mm3__chr6 137.00 Mbp_chr6_136997175_R GTCTCGTGGGCTCGGACCACTTCTCCCAAGCCTTG 59.8 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529594 CACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA
CGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC
mm1__chr1 223.86 Mbp_chr1_223864788 TGACCCATGCTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACCATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCC
TGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGAGCG
mm1__chr5 77.78 Mbp_chr5_77784926 ATATCACCGCGCTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTC
TTCCCCTCCATCGTT
mm3__chr5 61.83 Mbp_chr5_61832982 CCATCATCATGCCCTGGTGTCAGGGGTGCCCAACGATGGAGGGGGAACATGGGTTGGGGGACGTTGTGTCCAGCAAAGCC
AGCTTTGCACATGCTGGAGCCATTGTCAATGACCAGCC
mm3__chr1 237.93 Mbp_chr1_237926870 CTCCTCCACCAGTCACGATGGAAGAAGAGATCGCCACGCTTGCCATTGACAATGGCTCCAGCATGTGCAAAGCTGGCTTT
GCTGGGGACGATGCCCCCCAAGCTGTGTTTCCCTCCA
mm4__chr20 1.16 Mbp_chr20_1160623 GCTCCTCCACCGATCACAATGGAAGAAGAGATCACCATACTCGTCATTGACAATGGCTCCAGCATGTGCAAAGCTGGTTT
TGCTGGGGACGACGCCCCTGGAACCATGTTTCCC
mm4__chr17 81.51 Mbp_chr17_81512291 CCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAGCTTTGCACATGCCGGAGCCATTGTCAA
TGACCAGCG
mm4__chr3 139.49 Mbp_chr3_139493854 TGCCGGTCACAATGGAAGAAGAAATCACCATGCTTTTCATTGACATTGGCTCCAGCATGTACAAAGCTGGCTTTGCTGGG
GTGATGCTTCCCGAGCCATGTTCCCTTCCAT
mm3__chr19 9.52 Mbp_chr19_9518189 GCTGTCAGCTCACCATGGATGATGATATTGAGGTGCTTGTAGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTG
GGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
mm3__chr3 175.98 Mbp_chr3_175978516 GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC
ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGCTGACTACAAGCC
mm3__chr6 110.27 Mbp_chr6_110268509 AGAAAAGCAAATGTGGAGTAGCTATTAATCTGAGTCTCTGTTGAACTTCCAGCATGTGCATGGCCTGCTTGCTGCCATTC
CTGGGGGTAAGCGGCAGAAATCCAGCCTGGCTGCAAACAAGACAC
mm4__chr20 36.38 Mbp_chr20_36379162 TTCCAGAGACAGGGTGGTCAGAGAGGCCCTCTTTGTCCTCTCAAGCACACACTGGAGCTGGCCCTGCAAATGCTGGATGG
GTAAAGTGGCGGGAGGCAGAGGAGGCAGCGAGAACAAAGGTTG
mm2__chr9 6.83 Mbp_chr9_6834495 CCGCTACCAGCTCACCATAGATGATGATATTGCTACGCCCGTCGTCGACAACGGCTCCGGCATGTGGAAGGCTGGCTTCG
CAGGTGACGATGACCCCCGGCCGTCTTCCCCTTCATCGTGT
mm4__chr8 37.26 Mbp_chr8_37260983 CTCCCTTGGCTCCACATGTGGCACCCTCCGAGGGAGGTGGGAGTGTGGGGGCAGGGAGCTGGCCCAGCACATGCAGGTGG
CCCTGAGCTGCATCTCTGTCCTCC
mm4__chr2 59.22 Mbp_chr2_59216299 CTGACTGAGCAGTGACCCACACTAAGCATGGTGTATATTTAGGCATCTTTTCCTAACTGTTGCATATGCTTTCTTAGGAT
GACTTGCTGCCTGCATGTGCAGAGCTGCCTTCATGATTGGTCTTATGAGAATCTGGACCA
mm3__chr6 31.90 Mbp_chr6_31900245 GCGAGGGCACATGAAGATGAAGTGCTGCGCCCGCATGTGGAAGACCAGCTTCTCCACGCCCTGGAACACTTCCGGGCACT
TGGTGCACTTGATGTTCTTTAAGGGGTTTCCACCTGAGAAGC
mm4__chr20 57.01 Mbp_chr20_57011628 AAGGTAACAGGGGTGCGATGGGCTCCTCAGGCCCCTCAAACCCAGCATGTGCAACGCTGGCTCAGCCCTGACCTTCTACA
TTCCTATGTTCCCTCCATGCCCTTC
mm4__chr3 103.87 Mbp_chr3_103874331 TCAGGGTCAGGTTGGTGGTAATTGTAGCAATAGTGCCTGGTGCCAGCATGTGCTAGTCAGGCTGCAGTATTGGTGCCTGG
AACTGGCTGGGGTCAGTATGCTTCCC
mm3__chr9 123.11 Mbp_chr9_123110951 AAAGCAGTGCTCAGTACCCCATCTAAGAAATGGGGGTGCGCAGTGAACAGAACCTGCTTGTGCAAGGCCGGCCTGACCAT
GAAAAGTGATCGGGGGTAAGTAGAGGGATGCGTCTAATGGCA
mm4__chr16 84.25 Mbp_chr16_84252118 TCACATAGAAAGCCGTGGGGGAGGAGAAGTTGGGGCAGAGGATGTTGGCCTTGCGCATGCTGGGTTCAGGGCATCACTGG
AGACGTGGGGATTTGGAGTCCGGAGCACA
mm0__chr5 98.34 Mbp_chr5_98338783 GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT
CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm0__chr22 41.08 Mbp_chr22_41075176 ATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGACATCGTCACCCGCGAA
GCCGGCCTTGCACATGCCAGAGCTGTTGTCAACAACGAGCGCAGCAATA
mm4__chr12 57.18 Mbp_chr12_57181237 TGTCAACAACGGTGACTGCTCCCAGCTCTGCCTGCCCACGTCAGAGACGACCCGCTCCTGCATGTGCACAGCCGGCTATA
GCCTCCGGAGTGGCCAGCAGGCCTGCGAGGGTCAGTGCCTGGCTTTCCTCCCAGCCTTGT
mm1__chr11 1.80 Mbp_chr11_1804032 CACGATGGAGGGGAAGACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTTGTCTATGA
CAAGCAAGGTGATATTATCATCCATGGTGAGCTGGG
mm4__chrX 8.02 Mbp_chrX_8017680 TGTACAATGGGATGGCGACCCTGGGCTCTTGCTGGTCCCTGCATGTGCATGGCTCACTTCCACTGGGTGGAGTTTGTCTA
GATCCAGCCACAAAATCTCCACCATTTGATATGCAGTTTTGCCCCTGGA
mm1__chr22 15.72 Mbp_chr22_15721526 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr2 131.63 Mbp_chr2_131626178 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm1__chr2 131.26 Mbp_chr2_131263595 TGGATGATGATACCGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCC
CGGGCTGTCTTCCCTTCCATC
mm1__chr2 130.66 Mbp_chr2_130656789 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATC
mm1__chr2 130.46 Mbp_chr2_130463880 GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA
GCAC
mm1__chr2 130.08 Mbp_chr2_130075308 GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA
GCAC
mm1__chr14 19.40 Mbp_chr14_19402845 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm1__chr14 19.00 Mbp_chr14_18998907 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm4__chr5 176.79 Mbp_chr5_176792600 AGGTGCTCAAGTGTTCCCTGTGCCAGGAAGCCCACCTGGCACAAGCTGGAGGGTAGGCTGGCTGGAAGGCAGGGGCAACA
GGGCAAAAGGAGAAGGCAG
mm3__chr16 66.37 Mbp_chr16_66371351 CACTCCAGCAGGCACACCTCCTGCATGTTCTAGGCCGGCTCCTCCCAGCGCCCTGCCCCTCATCCCTGACCCCAGGCCTC
TGGGCTGGAGTCCAGAGGCTGCAGGAGGCTGGGCTGGGATCCTAGCTGAAAGCGGGTG
mm3__chrY 17.76 Mbp_chrY_17758062 AACGGTGGAGGGAAACATGGCTTGGGGAGCATCGTCCCCTGCAAAGCCAGCTTTGCACATGCAAGAGCCATTGTCAATGA
CGAGCGCAGCGATCTCTTCTTCTACTGCGATGGACAGAGGAGTGGGACAGAGGA
mm3__chrX 53.14 Mbp_chrX_53143850 GATGGAGGGGAACACAGCTCAGGGGCCATCCATCCCCAGCAAAGCCAGCTTTGCACATGCCAGAGCCATTGTCAATGATG
AGCGCGGCGATCTCTATTTCCATTGCAACCTGTGGAAGAGCA
mm3__chr2 57.76 Mbp_chr2_57764992 AGATTGCTGGCTCGTCAATGATAGTGGCTCTGGCATGTGCAAAGCTGGCTTTGCTGGGGAAGACACCCCCTGAGCCATGT
TTCTCTCCATCGTCAGGTGCCCCTGGCACCACAGTGTGATAGTGGGCATGGGCAAGAAG
mm2__chr15 43.99 Mbp_chr15_43990121 AATGAAGGGGAAGATGGCCCGGGGTGGGCATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGAC
AAGCATGGTGATATCATCATCCATGGTGAGCTGGC
mm4__chr2 21.06 Mbp_chr2_21057837 TCCTGTTAGCCAGACTTGGTTCTAGGTAACCATATAGAGCAATAAGAAGAAAGGCAGGCCTTCCACATGCTGGGTGATCT
ATGGAGCAGCTTACTCTTGTTATAGAACAAGTGGATCCTACACAGACCCA
mm3__chr22 39.97 Mbp_chr22_39971668 CAGGACGGGGTTGGAGAGACCCTGCCTGTGCAAGGCTGGCCTCCTAGTCAACACCTAGCTGAGACATTCATCTCTGTTCA
AATTAATATTCCTTGGGTCTTTCAAATGAATGTTTCAATGTGAGGAGCGAAGTACTGGGA
mm4__chr6 166.24 Mbp_chr6_166237177 TTCTCAGGTACCAGCACCCAGGGCAGCCGGCCCTGAAAATGCTGGGTAGGGACCGGAGACACCCCTCAAGGTCCTGCGCC
TTGGCTCGACCCTGAGGAGGTGAAGGAGGA
mm4__chrX 153.97 Mbp_chrX_153966444 ATTCAGCTGAGTCACTGGGCCTCGGACATGCCGAGCACTAGAGCTCCCCTGCCATTTCCCTAAAGCAGAGGGACAACACC
TGTGCCGCCCGCATGTGCAAGCTCGGATCCTGGCCCTCTGCCTCTTTCTCAT
mm4__chr21 45.12 Mbp_chr21_45121863 AGCCGTTGTTTTCTGTACGTATATGGTGTGTTTGACTTTTCCAGCATGTTCAAGGCTGTTTTCCCAGCTGTATCCCCTCC
TCT
mm2__chr18 62.44 Mbp_chr18_62442048 CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA
TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC
mm4__chr5 14.36 Mbp_chr5_14355139 TTGCTTTGGGAGTGAGTGGGCACCCTCTGTGTGTGGCCAGTTGCTCACCCCACCCCACCACCTGCATGTGGAAGGCAGGT
TACCATCTTAAGTAGAAATGTCCATGGATTGGATAATGTCATGGATGT
mm2__chrX 46.29 Mbp_chrX_46289065 TGGAGGGGAAGACAACTGGGGGGCATCATCGCCCATGAAGGCAGCCTTGCACATGCCAGAGCCTTTGTTGAAGACGAGAG
CAGTGATATCATCATTCATGGTGATCTGGCAGTGGGTGTGGACTGGTGATGGAG
mm4__chr6 9.20 Mbp_chr6_9202383 TGAAAGCCCTGATGTTGCCACCAGCCTGTGTCAGGCTGGCTTCTCCTGACTATGAAGAGAATCTCTATCATTCTTACATA
TTTACTAAATGGAGCTCTTTCTCCCCTTATAAATATTTTGTATTTTGACGTGGTTTCATC
mm4__chr17 77.10 Mbp_chr17_77095018 TCGTTCTCCTGTGTGGTGTCTTTCCTGTATGCAGAAGGCCGGCTTCCCCACAGGGGCTGGGGGTGACTTGTCTGTCCTTG
GAATATGAGTCTTTGCAAGCAGCTGCTGCCCATGTGCACAGTTCCTCGAGG
mm4__chr19 1.47 Mbp_chr19_1471777 TTTGAGGGTTGGGTGGATGGAGCTCAGAAGGAAACCCCAGCCCCACCACGGATGACACCATCCCTCCCGTCCCATCCCCA
GCATGGGCAAGGCCAGCCTTTCTGGCAGAAGGAGCTGTCC
mm4__chrUn_KI270741v1 141.53 Kbp_chrUn_KI270741v1_141532 GGAGAAGCTGGAAAGCGACTGCGGAGCTGCCGGCGGCAGGTGCGGGAAGGGGAGAAGCTGGCCTTGCACAGAAAGGCTGC
TCAGAGCGACACGGACAACGGCCAGGAGCTCCTCACGCAGCTGGAAGAACTC
mm4__chrUn_GL000219v1 22.62 Kbp_chrUn_GL000219v1_22623 GGAGAAGCTGGAAAGCGACTGCGGAGCTGCCGGCGGCAGGTGCGGGAAGGGGAGAAGCTGGCCTTGCACAGAAAGGCTGC
TCAGAGCGACACGGACAACGGCCAGGAGCTCCTCACGCAGCTGGAAGAACTC
mm4__chr13 113.43 Mbp_chr13_113433580 CCCACAAGATTTCCAGCCCAGTGTCCCGGGCAGGGCGCCCTCACAGCAGGCAGCCGGCAGGTGCAGGGTCAGCTTCTGTC
TCCTTTAAGCAGACGTGCAGATGGC
mm4__chr2 75.17 Mbp_chr2_75172685 CCCCAGGACAAGAGGCTCTTAAGTGGGAAAGAGAATGGAACGAATATATTCTTCTGGTCACCTCAGAAAATTATGACTTG
CCCCAGCATCTGCAATACCTGCTTCCTCTTATTAACAGCAGAAAGTACTTGCATTC
mm4__chr16 81.59 Mbp_chr16_81589548 TATGGCGAGTGCTTATGGGCAGGGCCAGGTCTCGCACCCCATGGCAGCCAGCCTGGCCCATGCCGGGCACTCTGGTCTCT
CTGTGGGACGAT
mm3__chr11 1.20 Mbp_chr11_1199701 CCCATCCCTCACTTCTGTGGGGGCCGCCGAGCGCCTCGGCACTGAGGGCGCCCCTCTGTCGGCACAGGACGAGGCCCGCA
TGAGCAAGGACGGCTGCTGCCGCTTCTGCCCGCCGCCCCCGCCCCCGTACCAGAACCGTG
mm4__chr17 46.95 Mbp_chr17_46952273 TGCTGGTGCCATTGTCTCTCCTTCCTGTGGAAAACCTGTGCCACGGGACTCAGCGAGGGCTGCCTCCTCCAGCAGGGTCA
GCGCCTGAAGCAGGCCTGGTCCATGCAGGACTGGGTCAGCAGCCCAAAGCAGTCGGTTTG
mm4__chr5 141.82 Mbp_chr5_141822764 TCTCCTTCACCACCTCCATCTTATTGCAGAAAAATCTCTTAAGAAGAAGACTGCCTTCCACATTCTGGTTTCGTACCTTT
ACTTACAAAGGGAGGAGTATATGTGGCTTAAACTGCTGTTTGGTTTGACTGTAAGCCCCA
mm4__chr19 5.81 Mbp_chr19_5807475 TCGGGAGATGGACAGGAGGCTCCCAAACCAGCCTTGCACTTGCAGGGCTGATAATGGCCTTGAAGGAAGCCTGAAGAAAG
AGTGGATATGAATGGGGCATGCCTGCAGGAAGGGTGTCTCGGAGATG
mm4__chr1 48.07 Mbp_chr1_48070084 ATTCCTGGCCAAGCATCCATCAAGACCCACATAAATATTTTCCCAGCATCTGCATGGCTGGCATCTAGTTGGCTAGGTTC
CAGGTGACAG
mm4__chr5 172.98 Mbp_chr5_172983758 GAGACAGCCAGGCGAAGGCGGGGCTCTGCCTCTGGGCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNTTGCACACGCTGGTCACGCGGTCAGCTATTGACACTTCCTGGTGGGA
mm4__chr15 65.98 Mbp_chr15_65982381 CATCCCTTGCCGTGGGTGCCAGGTGCGCAGAGCTCCTCGCAGCGCCATCCACGGAAGCCGGCGGCGCACACGCAGGCGCC
TGTGATGGGGTTACACA
mm4__chr17 21.36 Mbp_chr17_21364077 CTCCAGACAAGGGCAGTGGCGCAGAGGCCAGCCCTGCAGATGCAGGCCTTGCTGCCTCAGCCTGGGGCCTGCAGGTAAAA
CTGGTGACCTAATCTGAACTGGCTCACAGGAAGCT
mm4__chr1 228.86 Mbp_chr1_228863148 TCAGAGAGACATGGGACTACGAGTTATCCAAATTCAGGAATAGTCAGAACAAGTGAGTGAAACACACCTTTGCCTGCCTG
TGAAGGGCCAGCTTCTGCACGGGCACCAGAGACACAAAGA
mm4__chr13 38.68 Mbp_chr13_38679190 TGGGGAACTGGCTAAGCTTCTTGGAAGCAATTTAGGAACACTGCCTGAGATCCTAGATAGGGCACCAGCATGAACAAGGC
AGGCATCTTTCTAGGAGAGGGCTGGAAA
mm4__chr22 45.31 Mbp_chr22_45310735 CGACCACCTCCTAAGCTGTGGTCCCCGCGCCTGCAGGTGCAGGGCCGGGTTTAAGTTTTAATGTTCTGCTGAGACTCAGG
TGG
mm4__chr16 69.74 Mbp_chr16_69742673 GAAGAAGGGGAGCCACAGTCACACAAGGCCATGGCTGGTAAGGTGCAGCCGACCTTGCAGATCCTGGTGCAGGCAGATGA
CAGCGGGTGGTAATTGACTGCCT
mm4__chr8 117.65 Mbp_chr8_117650091 AGCTACTTGTCCCAAACGGGGAGTCTGTATGCACAGCCTGCAGGGTATGGTAAGTGACACTCTTTGTTTTCATCTCAGTT
CCCTGGATATGGAAGGCCAGCTTCATCCAAGGCACCATGGTGAA
mm4__chr22 24.54 Mbp_chr22_24543240 CAACTCAAGTCCTCCACCCCCAGGAAGCCCTCCTTGCCCATCCTGGTCTTCATGAGGTTTCTCTGACTCCTCCCCAGTAA
AGCAGTTGGAGAAAGGCCCAGA
mm2__chr2 73.90 Mbp_chr2_73901354 GTGTGAAGAGGAGACCACCGCGCTCGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTCGCAGGAGATGATGCCC
CCCGGGCTGTCTTCCCCTCCATTGTGGG
mm4__chr7 156.95 Mbp_chr7_156948914 AGGAACCTCAAACGCTCTGCCCTCGGGGCCCAGCGTCCAAACCCAGCCTGGGCAGGGCCGGCTCCTCAGGGGTCCTCGGC
CGCCTGGGGGCCACAGGCGTCTCCCTGGTCCGTATCCA
mm2__chr19 5.50 Mbp_chr19_5496885 CACGGCCCTTTGGTAACTCTCGCCAGTCATCTCATCTCACATGGCTCTTTGAGGGGCCACCACACAGAGGGAATCGCCAG
AGGCCGGCCTTTCACATGCAAGTGAGAACCGGGCTGTTTCCAACTGCCCAGGTC
mm4__chr13 110.21 Mbp_chr13_110208106 TGGGCGCTCTGGTTGTAATTGCCTCCACCAGCTTGGATGTCAGAGGAGAATCTGGCCTTCCACAGGCTGGCTACAGCAAT
GGCAGGCATCCCTTCTT
mm4__chr1 53.37 Mbp_chr1_53371273 ATTAACAGGCTCATCGGGGCGCTGGAGGAAGCAGCCCTGGCAGATGCAGGAGCCCAGCCTTCCCAGCCCCCACACGGTTA
TCAGCCTGGAGTTGCATCTCA
mm4__chr16 1.73 Mbp_chr16_1728981 CTGAGTGTTCTCCCATCGCACAACGCACACAGCAGTCTCCCAGACGGCCTTGCACTTGCCGGGCTGCTGCTGGTTGCCTC
AGGACCCAGAGTCCCAGCCTTGTCCTGGAACCCCCTCCCTCTGTGGTTACA
mm4__chr21 31.87 Mbp_chr21_31866258 GGAAAGCAGAGCCGAGTTGACCAGCAGGGGGCGCCGGCCTCTTTTTGTCTACATCTGTCCACCTGGCTGTGCCAGGCAGG
CTTCTGCAATCAGCACGCGTGCCCAGTTCTGTGACTGGTGA
mm4__chr1 115.48 Mbp_chr1_115479608 TTTTCCAGTTGCGAGTCCCACAGGTGCGTGCGTGCCAGTGCCCCTCGCTGTGCAGAAGCCAGCCTTGAACCTTCAGGAGA
CAGGAACCTTTGAGGGACCTCCACA
mm4__chr18 79.70 Mbp_chr18_79695954 GAGACTCAGCTGAAAGCCCTGAACCTGTCCTTGCACTTGCAGGTTGCAGAGTAAGAACGGGAAGCAGCAGG
mm4__chr1 31.70 Mbp_chr1_31701077 GCAGAGGGATAGGTGGGAGAAGACGGTGAAGACAGCTCCTAGAAGCTGGCCTTGCACTCTCTGGCTAGACTGGCTGGAAG
AAGGAATCCTGGGGCCCTGGGGCCCGGTGCCAATGACCCCACTGCACTCT
mm4__chr7 391.85 Kbp_chr7_391849 ACAGAGATCTGAGAGGGGCTTTCCCGGAGCCAGGCAGGGAGGCGGCCGTGCACCTGCGGGGGGACTGAGCCCCCCGGAGG
GACGAGCTGCATGATGACAGGGAGGG
mm3__chr10 69.02 Mbp_chr10_69022793 CCAGGGTGATATCACTGGGCTCCAGCAAGGGCAAGGCCAGCTTCGCAGGTGACGATGCCCCGTAGGCCGCCTTCCCCTCC
ATCTTG
mm4__chr19 8.13 Mbp_chr19_8133014 CAGGCTCACCCATGCAGTAGTGGCCGCCAGGCGCCAGCAGGAAGCCGGGTTTGCAGAGGCAGGAGAAGCTGCCATCCTCG
TTGAGACAC
mm4__chr19 41.91 Mbp_chr19_41912922 AGACGCAGCTACAGTCCATGGCGGAGCCGGCCCTGCAGAGGCCGGGAGGGACACACGGGGACGGGGTGGGCAGGAGAGAC
AGACACAGGTCAGCC
mm4__chr2 105.60 Mbp_chr2_105597301 AGGCCACCTCTCCCTGATAGCTGCCCATAGTTGAAGCTGGCCTTGCACAAAGGGGCCACCAGTCCCAGCTGGTGACACAG
TAG
mm4__chr18 79.77 Mbp_chr18_79771771 TTCCCAGAGCAAGAAAGCGTGGAGCCAGCAGCCGGCCTGGCAGAGGCTGGAGCGTGGGAGAAAGACCCCAGGACGGGTGG
CGTCCGGAAGAGAACACGGAA
mm4__chr22 30.71 Mbp_chr22_30712836 ACAGGTTGTATCTTGGGGTCATTATGGACATAACCTCAGCTCTTTATTCTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGTAAAGGAAGGAGCCCTGGT
mm4__chr1 210.58 Mbp_chr1_210583310 TCTGTAAGCAGTGACCACGCGAAGCCGGCCAGGCAGAGGCAGGCTTCGTGAAGACTCAACCTGACATAGTCACTGACGAG
AAGCAATCTTTTTAGTTTTGCTTTGCTGAGAAGGTGGTGCTGAT
mm4__chr16 3.67 Mbp_chr16_3671584 GACCACAAGCTTCAGAGGCCAGGCCAGCACCTGGAAGGCTCCTGAGGGCCCCCATGTGCAGGCCGGGCTTCCCCGACCAC
CTCTGCTCTCAGCTCCATGGGCCACGGCTAGGGCCCTCTGGGGGCAAAGGAGCAGGTAG
mm4__chr1 162.45 Mbp_chr1_162445219 CTCCAGTTCTGTGGGGCAGAAACATTTCAGAAAATAGGAAGTTGTGAGAGTTGAAATCTCAAACTGAATAAAAAAGAGGG
ACCAGCCAGGGCAAGGCTGGCTTCTGACTTCCCCAGTGGTCTCCAT
mm4__chr19 48.49 Mbp_chr19_48490784 AACGGAGGTGGGATCAGGATCCGGCAAGTGCCAGGCCGGAGTCTGAGCCCAGAGATGGGACAAAGATGGGCAAGAGTCGG
G
mm4__chr9 87.86 Mbp_chr9_87858440 TCAGCAAACCTGGAGAGCTGGGCAGGTGGTCTTTACCCAGCACCTTCAAGGCCGCCTTCTCTGGCCACAGGGAGCAGCCT
GGAACTGGGGCAGGGAGCACTGTTGGAACT
mm4__chr9 82.43 Mbp_chr9_82432288 CCCACCATCACCATCAGCTGGGCAGGTGGTCTTTACCCAGCACCTTCAAGGCCGCCTTCTCTGGCCACAGGGAGCAGCCC
GGAACTGGGGCAGGGAGCACCGTTGGAAGTGGGTCAGGCTTC
mm4__chr6 63.23 Mbp_chr6_63229492 AGCATCAGCAAGCATGGAGAGCTGGGCAGGTGGTCTTTACCCAGCACCTTCAAGGCCGCCTTCTCTGGCCACAGGGAGCA
GCCCGGAACTGGGGCAGGGACCACTGTTGGAAGTGGGTCAGGCTTC
mm4__chr2 68.53 Mbp_chr2_68528620 GCTGGGCAGGTGGTCTTTACCCAGCACCTTCAAGGCCGCCTTCTCTGGCCACAGGGAGCAGCCCGGAACTGGGGCAGGGA
GCACTGTTGGAAGTG
mm4__chr13 77.66 Mbp_chr13_77661782 AGAGCTGGGCAGGTAGTCTTTACCCAGCACCTTCAAGGCCGCCTTCTCTGGCCACAGGGAGCAGCCCGGAACTGGGGCAG
GGAGCACTGTTGGAAGTGA
mm4__chr9 134.55 Mbp_chr9_134551454 CATGACCTGGCATCCTCCGCAGGTGAGGGAGCCGGCCTTCCACCTGGAGGGGGCAGAGGGCAAAGGCTGCAGAGCCTCCG
CCGCGTCAGCCGCTGGTTGAACGGTCCAGG
mm4__chr9 127.93 Mbp_chr9_127928072 CCACTGGGAATTCTGCCCTCCCAGCCAGGGGTACCACTCCCACCCCTGCCCCACCCCTGCCGCAAGCTCACGACAGGAAG
AAGCTGGCCTTGCTCTTGGAGGTGCTGAGGAACTGCAGGTAGG
mm4__chr8 56.54 Mbp_chr8_56540406 CACTTACCTGCCTGCCAGACACATTGAAGCCTGCGATGGACATGCAGGGGAGAGGGAAAGGGGCTCCAACCTGGGGAGCC
CACATGCTAGTCAG
mm4__chr18 46.53 Mbp_chr18_46528931 ATCCAGCCAACAACCTCGAGCTCCAGCAGGCCTCCTGCTTGTCCAAGGCCGGCCTTCTGCTCCACCTCATCCCCTATGCA
GGATCCAGGGTGGGTGCATCAGCTAGGAG
mm4__chr15 77.57 Mbp_chr15_77572054 ATCGTGGTGTCACTTGGCATAGCAAGAAGCATCCCCCAGGATCTCGACTGGCGTTGGAGTGGTGTGGGGCCCAGGTAGAG
TTGGGCCAGCAGGTCCAAGTCCGGCTTTGGGGTCAAAGGGCAAGGAGAA
mm4__chr11 7.57 Mbp_chr11_7565658 CTACATGAACCCCTTCCCGGTGCTCCATCTCATCGAGGACTTGAGGCTGGCCTTGGAGATGCTGGAGCTTCCTCAGGAGA
GAGCA
mm4__chr10 130.41 Mbp_chr10_130409532 AGTGATGTGTGCATGGCTGTTTCCTTGCAACAAAGAATTCCATTTAGCAGACACGCGCCTCCCTGTAAGTCCAAGGCCAG
CTTCCAGCAGCAGGGACGGGGGTTGTAAAGACAAACAAGGCATGGACCAAGGA
mm3__chr6 137.00 Mbp_chr6_136997175 GGCTGGAGGAACATCAGTCCTGCTGAGATGGTCAAAGTAATGGCCTGCATGGCCAAGGCCGCCTTCAAGCACATGTCACT
GAGGACCCAAGGCTTGGGAGAAGTGGT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.