← return to the list of all guides

Contents:

PCR primers for off-targets of CGGCGCCCCCTGGCGGCCTA AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529732_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529732_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr7 1.95 Mbp_chr7_1952112_F Primer3: not found at this Tm N.d. 0.56
mm4__chr7 1.95 Mbp_chr7_1952112_R Primer3: not found at this Tm N.d. 0.56
mm4__chr5 134.58 Mbp_chr5_134577956_F TCGTCGGCAGCGTCGCTGGGAGGCAACGCTTATA 60.1 0.51
mm4__chr5 134.58 Mbp_chr5_134577956_R GTCTCGTGGGCTCGGGGGTTGGGGAAGGAGGAATC 59.7 0.51
mm2__chr19 18.39 Mbp_chr19_18388563_F TCGTCGGCAGCGTCACTGCTGGCAGAATCTTCGT 59.6 0.49
mm2__chr19 18.39 Mbp_chr19_18388563_R GTCTCGTGGGCTCGGGACAGTGGTCCCCGTTGC 60.6 0.49
mm4__chr9 16.42 Mbp_chr9_16419240_F TCGTCGGCAGCGTCCCACCATTGCTCCCAGACAA 60.2 0.38
mm4__chr9 16.42 Mbp_chr9_16419240_R GTCTCGTGGGCTCGGTCTCGATGACATTGACGGGG 59.5 0.38
mm4__chr1 167.44 Mbp_chr1_167442325_F Primer3: not found at this Tm N.d. 0.35
mm4__chr1 167.44 Mbp_chr1_167442325_R Primer3: not found at this Tm N.d. 0.35
mm4__chr9 126.12 Mbp_chr9_126117319_F Primer3: not found at this Tm N.d. 0.33
mm4__chr9 126.12 Mbp_chr9_126117319_R Primer3: not found at this Tm N.d. 0.33
mm4__chr1 44.02 Mbp_chr1_44020815_F TCGTCGGCAGCGTCTATGAGAACGCGTCAGGGTG 59.8 0.30
mm4__chr1 44.02 Mbp_chr1_44020815_R GTCTCGTGGGCTCGGTGTCCAAATCCAAGGAGGCC 59.9 0.30
mm3__chr9 136.36 Mbp_chr9_136363835_F TCGTCGGCAGCGTCTTCTTGGGCTTCCGTCTTCC 59.9 0.29
mm3__chr9 136.36 Mbp_chr9_136363835_R GTCTCGTGGGCTCGGGTGTCCTCGGGGTGCGAG 62.4 0.29
mm4__chr20 46.02 Mbp_chr20_46023037_F Primer3: not found at this Tm N.d. 0.28
mm4__chr20 46.02 Mbp_chr20_46023037_R Primer3: not found at this Tm N.d. 0.28
mm4__chr8 106.82 Mbp_chr8_106817530_F TCGTCGGCAGCGTCGCTTTGGCATGTGGGTGATG 60.1 0.26
mm4__chr8 106.82 Mbp_chr8_106817530_R GTCTCGTGGGCTCGGGCTTTTCACAGCTGTTGCCT 59.6 0.26
mm4__chr10 80.17 Mbp_chr10_80169256_F TCGTCGGCAGCGTCCATAGGGAGGAACAGGCTGC 60.1 0.26
mm4__chr10 80.17 Mbp_chr10_80169256_R GTCTCGTGGGCTCGGAACATGCCCAACCTGTACCC 60.2 0.26
mm4__chr1 27.55 Mbp_chr1_27552129_F TCGTCGGCAGCGTCAGGTAGTCAGGAGAGCTGCA 59.9 0.25
mm4__chr1 27.55 Mbp_chr1_27552129_R GTCTCGTGGGCTCGGTTCTCGCACCCTCATGTCAG 59.7 0.25
mm4__chr4 35.04 Mbp_chr4_35038533_F Primer3: not found at this Tm N.d. 0.24
mm4__chr4 35.04 Mbp_chr4_35038533_R Primer3: not found at this Tm N.d. 0.24
mm3__chr1 110.62 Mbp_chr1_110619505_F TCGTCGGCAGCGTCAAAAGACAGACGCACGAGCT 60.2 0.21
mm3__chr1 110.62 Mbp_chr1_110619505_R GTCTCGTGGGCTCGGAGCCACAGTGTGCAGATACC 60.0 0.21
mm4__chr2 121.07 Mbp_chr2_121071185_F TCGTCGGCAGCGTCCCTGGTGAAAGAAGGGCAGT 59.8 0.21
mm4__chr2 121.07 Mbp_chr2_121071185_R GTCTCGTGGGCTCGGCTGTCTTGGGGAAGCCCTG 60.0 0.21
mm4__chr11 120.51 Mbp_chr11_120511226_F TCGTCGGCAGCGTCTGAGGCCGCAGTCTCTTCTA 60.3 0.21
mm4__chr11 120.51 Mbp_chr11_120511226_R GTCTCGTGGGCTCGGGCCCCGAGGAGTTATTAGCC 59.9 0.21
mm4__chr19 43.60 Mbp_chr19_43598824_F TCGTCGGCAGCGTCAGGTCTTGGAGACTCCTGCT 59.8 0.21
mm4__chr19 43.60 Mbp_chr19_43598824_R GTCTCGTGGGCTCGGCATGTGACGCTCCTGGAACT 60.0 0.21
mm4__chr11 65.00 Mbp_chr11_64996960_F TCGTCGGCAGCGTCACAGAGGTGCATGGCTTAGG 59.7 0.20
mm4__chr11 65.00 Mbp_chr11_64996960_R GTCTCGTGGGCTCGGACTGAAACTTGGCCCTCTGG 59.8 0.20
mm4__chr8 41.58 Mbp_chr8_41578018_F Primer3: not found at this Tm N.d. 0.20
mm4__chr8 41.58 Mbp_chr8_41578018_R Primer3: not found at this Tm N.d. 0.20
mm2__chr3 50.26 Mbp_chr3_50260133_F TCGTCGGCAGCGTCCTCGACTGGGTGGGGCTC 61.7 0.19
mm2__chr3 50.26 Mbp_chr3_50260133_R GTCTCGTGGGCTCGGCTGACGTCAGCCTCTGTGAC 60.3 0.19
mm4__chr4 99.95 Mbp_chr4_99946343_F Primer3: not found at this Tm N.d. 0.18
mm4__chr4 99.95 Mbp_chr4_99946343_R Primer3: not found at this Tm N.d. 0.18
mm4__chr17 46.89 Mbp_chr17_46891086_F TCGTCGGCAGCGTCAAGCACCTGATGGGGAGAGA 60.2 0.18
mm4__chr17 46.89 Mbp_chr17_46891086_R GTCTCGTGGGCTCGGAGGCTGAGACTAGCCAGACA 59.9 0.18
mm3__chr15 68.88 Mbp_chr15_68876010_F TCGTCGGCAGCGTCAGTCCATGTGTAGCTGCAAGT 59.6 0.18
mm3__chr15 68.88 Mbp_chr15_68876010_R GTCTCGTGGGCTCGGTCTGTGCCAACCTCCTTGAC 59.8 0.18
mm4__chr3 51.94 Mbp_chr3_51942210_F TCGTCGGCAGCGTCAAGTCGGAGCAAGAGCCTTC 60.0 0.17
mm4__chr3 51.94 Mbp_chr3_51942210_R GTCTCGTGGGCTCGGGGGAGGAGTCACGTTCCAGA 60.8 0.17
mm4__chr10 120.04 Mbp_chr10_120041866_F Primer3: not found at this Tm N.d. 0.17
mm4__chr10 120.04 Mbp_chr10_120041866_R Primer3: not found at this Tm N.d. 0.17
mm3__chr4 674.38 Kbp_chr4_674378_F TCGTCGGCAGCGTCCACAAGCCAGCAAAGCCTTG 60.5 0.17
mm3__chr4 674.38 Kbp_chr4_674378_R GTCTCGTGGGCTCGGCCTCACGTGACGTCACCG 60.4 0.17
mm4__chr14 104.56 Mbp_chr14_104559907_F TCGTCGGCAGCGTCACCAGAGAAGTCCCTCCCTC 59.9 0.16
mm4__chr14 104.56 Mbp_chr14_104559907_R GTCTCGTGGGCTCGGCCCCTTCCCCATCTGTCAAG 59.7 0.16
mm4__chr7 6.27 Mbp_chr7_6272328_F Primer3: not found at this Tm N.d. 0.15
mm4__chr7 6.27 Mbp_chr7_6272328_R Primer3: not found at this Tm N.d. 0.15
mm4__chr16 1.90 Mbp_chr16_1900522_F TCGTCGGCAGCGTCTGCTCTTCTCCAGAACGCAG 60.0 0.15
mm4__chr16 1.90 Mbp_chr16_1900522_R GTCTCGTGGGCTCGGCCACCCGCATGCTACATTTG 59.8 0.15
mm4__chr3 50.41 Mbp_chr3_50412549_F Primer3: not found at this Tm N.d. 0.14
mm4__chr3 50.41 Mbp_chr3_50412549_R Primer3: not found at this Tm N.d. 0.14
mm4__chr13 80.34 Mbp_chr13_80343017_F TCGTCGGCAGCGTCGAATCCATTGAGCCGACGGT 60.4 0.14
mm4__chr13 80.34 Mbp_chr13_80343017_R GTCTCGTGGGCTCGGGTGTCCCGGAGAAGAAAGCA 59.9 0.14
mm4__chr16 10.26 Mbp_chr16_10256972_F TCGTCGGCAGCGTCAAGGGGAAGGACTTTGTGGG 59.5 0.14
mm4__chr16 10.26 Mbp_chr16_10256972_R GTCTCGTGGGCTCGGGAGGAAAGTTACCGAGCCCG 60.4 0.14
mm4__chr17 9.00 Mbp_chr17_8998194_F TCGTCGGCAGCGTCCAGCATTTACTGTGCGAGCC 59.9 0.14
mm4__chr17 9.00 Mbp_chr17_8998194_R GTCTCGTGGGCTCGGTGAAGGTGCCTCAACCCATC 59.9 0.14
mm4__chr1 3.50 Mbp_chr1_3502551_F TCGTCGGCAGCGTCGTGGGATGGGGCTAGAATGG 59.8 0.14
mm4__chr1 3.50 Mbp_chr1_3502551_R GTCTCGTGGGCTCGGCCTGGGGTCTCATTCACCTG 59.7 0.14
mm4__chr14 100.24 Mbp_chr14_100240416_F TCGTCGGCAGCGTCCAGGGATTGCTGCCTCCG 60.5 0.13
mm4__chr14 100.24 Mbp_chr14_100240416_R GTCTCGTGGGCTCGGTTGGGAGTCCGGGGAAGTT 60.4 0.13
mm4__chr19 55.09 Mbp_chr19_55092816_F TCGTCGGCAGCGTCTCCACTCCTTTCCGACCTTG 59.3 0.13
mm4__chr19 55.09 Mbp_chr19_55092816_R GTCTCGTGGGCTCGGGAGGTGCCTGGGCTGATTC 60.4 0.13
mm4__chr1 6.70 Mbp_chr1_6701951_F Primer3: not found at this Tm N.d. 0.13
mm4__chr1 6.70 Mbp_chr1_6701951_R Primer3: not found at this Tm N.d. 0.13
mm4__chr17 46.19 Mbp_chr17_46193563_F Primer3: not found at this Tm N.d. 0.12
mm4__chr17 46.19 Mbp_chr17_46193563_R Primer3: not found at this Tm N.d. 0.12
mm4__chr8 69.98 Mbp_chr8_69980262_F Primer3: not found at this Tm N.d. 0.12
mm4__chr8 69.98 Mbp_chr8_69980262_R Primer3: not found at this Tm N.d. 0.12
mm4__chr19 50.48 Mbp_chr19_50480803_F Primer3: not found at this Tm N.d. 0.12
mm4__chr19 50.48 Mbp_chr19_50480803_R Primer3: not found at this Tm N.d. 0.12
mm4__chr11 61.89 Mbp_chr11_61891689_F Primer3: not found at this Tm N.d. 0.12
mm4__chr11 61.89 Mbp_chr11_61891689_R Primer3: not found at this Tm N.d. 0.12
mm4__chr4 76.31 Mbp_chr4_76307314_F Primer3: not found at this Tm N.d. 0.12
mm4__chr4 76.31 Mbp_chr4_76307314_R Primer3: not found at this Tm N.d. 0.12
mm4__chr13 75.64 Mbp_chr13_75636133_F Primer3: not found at this Tm N.d. 0.12
mm4__chr13 75.64 Mbp_chr13_75636133_R Primer3: not found at this Tm N.d. 0.12
mm3__chr12 53.32 Mbp_chr12_53321676_F TCGTCGGCAGCGTCAAAGAAGGACGAGTACGCGG 60.1 0.12
mm3__chr12 53.32 Mbp_chr12_53321676_R GTCTCGTGGGCTCGGCACTCCAGCCTGGTAACCAA 59.6 0.12
mm4__chr19 38.40 Mbp_chr19_38403115_F TCGTCGGCAGCGTCGGGAAGATGCCGATTGGTCA 60.1 0.12
mm4__chr19 38.40 Mbp_chr19_38403115_R GTCTCGTGGGCTCGGCGCCTCCATGAAAGTGTGGT 60.6 0.12
mm3__chr6 111.87 Mbp_chr6_111872833_F Primer3: not found at this Tm N.d. 0.11
mm3__chr6 111.87 Mbp_chr6_111872833_R Primer3: not found at this Tm N.d. 0.11
mm4__chr6 147.51 Mbp_chr6_147508202_F Primer3: not found at this Tm N.d. 0.11
mm4__chr6 147.51 Mbp_chr6_147508202_R Primer3: not found at this Tm N.d. 0.11
mm4__chr12 76.08 Mbp_chr12_76084302_F TCGTCGGCAGCGTCGGAAAAGACGGTGGAGCTCA 59.9 0.10
mm4__chr12 76.08 Mbp_chr12_76084302_R GTCTCGTGGGCTCGGGCCGACCAGCGCTTTTATC 59.6 0.10
mm3__chr1 112.72 Mbp_chr1_112718842_F TCGTCGGCAGCGTCGACCTCCTCTGAGTTGCCAC 60.0 0.10
mm3__chr1 112.72 Mbp_chr1_112718842_R GTCTCGTGGGCTCGGCTCGGCTGAACGGATGGG 60.2 0.10
mm3__chr9 127.79 Mbp_chr9_127785126_F TCGTCGGCAGCGTCCTTGCTCGTCGCCGCTAG 60.9 0.10
mm3__chr9 127.79 Mbp_chr9_127785126_R GTCTCGTGGGCTCGGGGCCCTGGGAGGAGAGAA 59.9 0.10
mm3__chr1 8.85 Mbp_chr1_8849074_F TCGTCGGCAGCGTCGAGGCCCAGGGTCTTGGG 61.7 0.10
mm3__chr1 8.85 Mbp_chr1_8849074_R GTCTCGTGGGCTCGGACTGTGCAGAAGGTCAGCTG 60.2 0.10
mm4__chr2 170.97 Mbp_chr2_170973586_F Primer3: not found at this Tm N.d. 0.10
mm4__chr2 170.97 Mbp_chr2_170973586_R Primer3: not found at this Tm N.d. 0.10
mm4__chr7 101.17 Mbp_chr7_101172400_F Primer3: not found at this Tm N.d. 0.09
mm4__chr7 101.17 Mbp_chr7_101172400_R Primer3: not found at this Tm N.d. 0.09
mm4__chr12 576.27 Kbp_chr12_576266_F TCGTCGGCAGCGTCCTAAAGCTGCAGCACCGAGA 60.3 0.09
mm4__chr12 576.27 Kbp_chr12_576266_R GTCTCGTGGGCTCGGGGGTTGAGCTGGGGCCTC 62.4 0.09
mm4__chr22 42.61 Mbp_chr22_42614906_F TCGTCGGCAGCGTCGAAAGCAGTCGAGACCCCG 60.4 0.09
mm4__chr22 42.61 Mbp_chr22_42614906_R GTCTCGTGGGCTCGGCGGTTCTTGCCTGCTTTGAG 59.7 0.09
mm4__chr7 100.69 Mbp_chr7_100693678_F TCGTCGGCAGCGTCGGAGAACTCCGGGAAGCG 59.8 0.09
mm4__chr7 100.69 Mbp_chr7_100693678_R GTCTCGTGGGCTCGGGCTCCTAACGGTCGCCCC 62.5 0.09
mm4__chr7 55.26 Mbp_chr7_55255008_F Primer3: not found at this Tm N.d. 0.09
mm4__chr7 55.26 Mbp_chr7_55255008_R Primer3: not found at this Tm N.d. 0.09
mm4__chrX 110.17 Mbp_chrX_110168060_F TCGTCGGCAGCGTCCTGTTGGCTCAGTCACTGCT 60.2 0.09
mm4__chrX 110.17 Mbp_chrX_110168060_R GTCTCGTGGGCTCGGCTCCCTCCATCCTCTCTGCT 60.1 0.09
mm2__chr11 61.76 Mbp_chr11_61758136_F TCGTCGGCAGCGTCGTGGGGAGTACAGTTGGGTC 59.6 0.09
mm2__chr11 61.76 Mbp_chr11_61758136_R GTCTCGTGGGCTCGGACTTCCCTCACTGATGCGAC 59.7 0.09
mm4__chr2 71.94 Mbp_chr2_71944374_F TCGTCGGCAGCGTCGGCTCAGACAAGCAGGGATT 60.0 0.09
mm4__chr2 71.94 Mbp_chr2_71944374_R GTCTCGTGGGCTCGGCTGAGCTTTCCTGATGCCCA 60.0 0.09
mm3__chr13 113.39 Mbp_chr13_113394138_F TCGTCGGCAGCGTCTTCCTCTTGCAGCAGCCG 60.3 0.09
mm3__chr13 113.39 Mbp_chr13_113394138_R GTCTCGTGGGCTCGGCCTGCAGGGACAGGCCTC 62.1 0.09
mm4__chr19 2.25 Mbp_chr19_2252503_F Primer3: not found at this Tm N.d. 0.08
mm4__chr19 2.25 Mbp_chr19_2252503_R Primer3: not found at this Tm N.d. 0.08
mm4__chr11 112.28 Mbp_chr11_112280090_F TCGTCGGCAGCGTCGCACTTGGCTCTCAGGGATT 60.0 0.08
mm4__chr11 112.28 Mbp_chr11_112280090_R GTCTCGTGGGCTCGGCTTCTTTGCCGCCCATCCTA 60.1 0.08
mm4__chr14 23.37 Mbp_chr14_23365336_F Primer3: not found at this Tm N.d. 0.08
mm4__chr14 23.37 Mbp_chr14_23365336_R Primer3: not found at this Tm N.d. 0.08
mm3__chr4 8.47 Mbp_chr4_8473264_F TCGTCGGCAGCGTCCCTGAGCATGACCCATGAGG 60.1 0.08
mm3__chr4 8.47 Mbp_chr4_8473264_R GTCTCGTGGGCTCGGCCATACCAGCCAGCATGGAT 59.8 0.08
mm4__chr9 136.44 Mbp_chr9_136439887_F Primer3: not found at this Tm N.d. 0.08
mm4__chr9 136.44 Mbp_chr9_136439887_R Primer3: not found at this Tm N.d. 0.08
mm4__chr6 64.30 Mbp_chr6_64300256_F TCGTCGGCAGCGTCGCTTGGTAGTGGGGAAGCTC 60.3 0.07
mm4__chr6 64.30 Mbp_chr6_64300256_R GTCTCGTGGGCTCGGACTTGCCTAGGTGCTGCTTT 59.8 0.07
mm4__chr16 87.66 Mbp_chr16_87661731_F Primer3: not found at this Tm N.d. 0.07
mm4__chr16 87.66 Mbp_chr16_87661731_R Primer3: not found at this Tm N.d. 0.07
mm4__chr1 225.48 Mbp_chr1_225475082_F TCGTCGGCAGCGTCAATCCCAAACTGAGAGGCCG 60.0 0.07
mm4__chr1 225.48 Mbp_chr1_225475082_R GTCTCGTGGGCTCGGCGACCCCTGGCGGTCATG 62.8 0.07
mm4__chr16 33.84 Mbp_chr16_33844703_F TCGTCGGCAGCGTCATGACAGAAAGCCAGCCCTC 60.0 0.07
mm4__chr16 33.84 Mbp_chr16_33844703_R GTCTCGTGGGCTCGGCAGGCACAAACCTCCCTGAA 60.1 0.07
mm4__chr16 33.01 Mbp_chr16_33009677_F TCGTCGGCAGCGTCGAAGTCAGTGAGAGCCCAGG 59.7 0.07
mm4__chr16 33.01 Mbp_chr16_33009677_R GTCTCGTGGGCTCGGATGACAGAAAGCCAGCCCTC 60.0 0.07
mm4__chr20 3.71 Mbp_chr20_3711063_F TCGTCGGCAGCGTCCAACTTGGGTTGCAGCTGTG 60.2 0.07
mm4__chr20 3.71 Mbp_chr20_3711063_R GTCTCGTGGGCTCGGCAAGGGAGGGATGGTTCAGG 59.7 0.07
mm4__chr22 41.92 Mbp_chr22_41919574_F TCGTCGGCAGCGTCGGCAACTCTGACTCAGGCTC 60.3 0.07
mm4__chr22 41.92 Mbp_chr22_41919574_R GTCTCGTGGGCTCGGACGTACACTGAGGGCTTTGG 59.9 0.07
mm4__chr11 133.95 Mbp_chr11_133946643_F TCGTCGGCAGCGTCAGGACATGGAGGTGAGGGG 60.3 0.06
mm4__chr11 133.95 Mbp_chr11_133946643_R GTCTCGTGGGCTCGGAGGCTGGCTCTGTCATTTCC 60.0 0.06
mm4__chr12 120.58 Mbp_chr12_120583559_F TCGTCGGCAGCGTCCACTCCCCACCCAAGAACAA 59.8 0.06
mm4__chr12 120.58 Mbp_chr12_120583559_R GTCTCGTGGGCTCGGGGGTGTCTTGCTCTCTCCG 59.7 0.06
mm4__chr19 19.63 Mbp_chr19_19627190_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 19.63 Mbp_chr19_19627190_R Primer3: not found at this Tm N.d. 0.06
mm4__chr11 6.26 Mbp_chr11_6259986_F TCGTCGGCAGCGTCCTAAAGCTGAACCGGAGCGT 60.3 0.05
mm4__chr11 6.26 Mbp_chr11_6259986_R GTCTCGTGGGCTCGGACACTGCTGCTGTTGAGGAG 60.2 0.05
mm4__chr7 156.95 Mbp_chr7_156949636_F Primer3: not found at this Tm N.d. 0.05
mm4__chr7 156.95 Mbp_chr7_156949636_R Primer3: not found at this Tm N.d. 0.05
mm4__chr9 137.24 Mbp_chr9_137236107_F TCGTCGGCAGCGTCCTACCTGCTGCTCGGATTCC 60.2 0.05
mm4__chr9 137.24 Mbp_chr9_137236107_R GTCTCGTGGGCTCGGCTGCAGGACAGTAACCAGGA 59.0 0.05
mm3__chr7 78.04 Mbp_chr7_78040366_F TCGTCGGCAGCGTCCAAGCAGAGCGAGGATGAGG 60.5 0.05
mm3__chr7 78.04 Mbp_chr7_78040366_R GTCTCGTGGGCTCGGGTAGAGAACGCGGCGAGAA 59.8 0.05
mm4__chr1 244.37 Mbp_chr1_244367647_F TCGTCGGCAGCGTCCAGAGAGCAGAAGACAGCCC 60.1 0.05
mm4__chr1 244.37 Mbp_chr1_244367647_R GTCTCGTGGGCTCGGCTCCTGCCTAGTGCTCTGTG 59.8 0.05
mm4__chr5 7.87 Mbp_chr5_7869332_F TCGTCGGCAGCGTCGCCCTGCACTAATCTCCTGG 60.1 0.05
mm4__chr5 7.87 Mbp_chr5_7869332_R GTCTCGTGGGCTCGGAAAGGCCAAGTCCGAGCC 59.9 0.05
mm3__chr3 3.18 Mbp_chr3_3179778_F TCGTCGGCAGCGTCGGTGCCGAAGACTCCGAG 59.8 0.05
mm3__chr3 3.18 Mbp_chr3_3179778_R GTCTCGTGGGCTCGGAGGCCATCACTTTCAGGCTT 59.5 0.05
mm4__chr17 31.53 Mbp_chr17_31525159_F TCGTCGGCAGCGTCCTGTCCTCAGGGTGCATGAG 60.1 0.04
mm4__chr17 31.53 Mbp_chr17_31525159_R GTCTCGTGGGCTCGGGGTAGCCTTCTCCCTCTCCA 60.0 0.04
mm4__chr8 22.55 Mbp_chr8_22551974_F TCGTCGGCAGCGTCTTCTCCTTGCCCTTCCTCCT 60.1 0.04
mm4__chr8 22.55 Mbp_chr8_22551974_R GTCTCGTGGGCTCGGCTTGCCCGGACCTACCTTC 59.7 0.04
mm4__chr1 46.53 Mbp_chr1_46532983_F TCGTCGGCAGCGTCAATGGCACTGAAGGAAGGGG 59.9 0.04
mm4__chr1 46.53 Mbp_chr1_46532983_R GTCTCGTGGGCTCGGGAAGGAGTCCAGCTCAACCC 60.0 0.04
mm4__chr3 128.61 Mbp_chr3_128608519_F TCGTCGGCAGCGTCGACACACAGCCCAGTAGTGG 60.3 0.04
mm4__chr3 128.61 Mbp_chr3_128608519_R GTCTCGTGGGCTCGGGAGGCCGGCAGGGATTGG 62.8 0.04
mm4__chr17 7.01 Mbp_chr17_7014897_F TCGTCGGCAGCGTCGTCAGCGTCCACAAAGGTTC 59.4 0.04
mm4__chr17 7.01 Mbp_chr17_7014897_R GTCTCGTGGGCTCGGGAAGAGCCGCGAGCCAAG 61.1 0.04
mm4__chr8 123.16 Mbp_chr8_123160593_F TCGTCGGCAGCGTCCTGGGCTGATCCTGCCTTG 60.4 0.04
mm4__chr8 123.16 Mbp_chr8_123160593_R GTCTCGTGGGCTCGGAAAAGCCGAAACCTGCGGG 61.5 0.04
mm4__chr9 69.04 Mbp_chr9_69035712_F TCGTCGGCAGCGTCCCCCACAGAAGAGTGCCTG 60.0 0.04
mm4__chr9 69.04 Mbp_chr9_69035712_R GTCTCGTGGGCTCGGGAGAGTCCACATGCTGCTCC 60.4 0.04
mm4__chr3 172.04 Mbp_chr3_172040686_F Primer3: not found at this Tm N.d. 0.04
mm4__chr3 172.04 Mbp_chr3_172040686_R Primer3: not found at this Tm N.d. 0.04
mm3__chr19 46.72 Mbp_chr19_46716289_F Primer3: not found at this Tm N.d. 0.04
mm3__chr19 46.72 Mbp_chr19_46716289_R Primer3: not found at this Tm N.d. 0.04
mm4__chr6 159.83 Mbp_chr6_159827429_F Primer3: not found at this Tm N.d. 0.04
mm4__chr6 159.83 Mbp_chr6_159827429_R Primer3: not found at this Tm N.d. 0.04
mm4__chr17 28.35 Mbp_chr17_28347146_F TCGTCGGCAGCGTCGGCTGAGTTCCTGTGCTTCT 59.9 0.04
mm4__chr17 28.35 Mbp_chr17_28347146_R GTCTCGTGGGCTCGGTCACAGTTCCAGCCCTTGTT 59.4 0.04
mm3__chr14 69.73 Mbp_chr14_69726928_F TCGTCGGCAGCGTCCTGCAGCAGAGGCCGGAG 62.8 0.04
mm3__chr14 69.73 Mbp_chr14_69726928_R GTCTCGTGGGCTCGGTTCTTCCCAGGGAAAGTGGC 59.8 0.04
mm4__chr10 48.52 Mbp_chr10_48524454_F Primer3: not found at this Tm N.d. 0.04
mm4__chr10 48.52 Mbp_chr10_48524454_R Primer3: not found at this Tm N.d. 0.04
mm4__chr6 147.51 Mbp_chr6_147509199_F Primer3: not found at this Tm N.d. 0.03
mm4__chr6 147.51 Mbp_chr6_147509199_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 76.21 Mbp_chr11_76208948_F TCGTCGGCAGCGTCCACCTTCCGCCTGAGCTC 60.1 0.03
mm4__chr11 76.21 Mbp_chr11_76208948_R GTCTCGTGGGCTCGGGAAGAGGACTTGACCCCAGC 60.0 0.03
mm4__chr12 121.50 Mbp_chr12_121500058_F TCGTCGGCAGCGTCTACCTCCCGTTTCCCTCTGT 59.8 0.03
mm4__chr12 121.50 Mbp_chr12_121500058_R GTCTCGTGGGCTCGGCTTTTCCCGGAGCCTGTCAT 60.0 0.03
mm4__chr1 112.28 Mbp_chr1_112283901_F TCGTCGGCAGCGTCGAGTGGTCTTGGTCTCAGGG 59.3 0.03
mm4__chr1 112.28 Mbp_chr1_112283901_R GTCTCGTGGGCTCGGCTCTCCAGACATCAGGAAGACA 58.9 0.03
mm4__chr16 66.27 Mbp_chr16_66270596_F TCGTCGGCAGCGTCCAGGGTCCGGGTTCGCTG 62.7 0.03
mm4__chr16 66.27 Mbp_chr16_66270596_R GTCTCGTGGGCTCGGAACCTTCTGCTCCAGGAAGG 59.3 0.03
mm4__chr11 47.19 Mbp_chr11_47186120_F TCGTCGGCAGCGTCCCTGAAGACGGCTCAGCC 60.1 0.03
mm4__chr11 47.19 Mbp_chr11_47186120_R GTCTCGTGGGCTCGGGACGGGAAGGGCCGTAGC 62.5 0.03
mm4__chr19 921.79 Kbp_chr19_921791_F Primer3: not found at this Tm N.d. 0.03
mm4__chr19 921.79 Kbp_chr19_921791_R Primer3: not found at this Tm N.d. 0.03
mm3__chr1 37.51 Mbp_chr1_37514789_F Primer3: not found at this Tm N.d. 0.03
mm3__chr1 37.51 Mbp_chr1_37514789_R Primer3: not found at this Tm N.d. 0.03
mm4__chr12 13.28 Mbp_chr12_13278332_F TCGTCGGCAGCGTCTTGAAGCTCCGCAGAGAGTG 60.0 0.03
mm4__chr12 13.28 Mbp_chr12_13278332_R GTCTCGTGGGCTCGGATGTACAGGAGACCGGCTTG 59.4 0.03
mm3__chr1 40.37 Mbp_chr1_40373901_F TCGTCGGCAGCGTCCCCGCAGCCTCTCTTGGA 61.3 0.03
mm3__chr1 40.37 Mbp_chr1_40373901_R GTCTCGTGGGCTCGGAGAGCCCTCTCCTCCGGC 62.8 0.03
mm4__chr9 137.02 Mbp_chr9_137023710_F TCGTCGGCAGCGTCACCAGCTGTGCCTTTAGGTG 60.2 0.03
mm4__chr9 137.02 Mbp_chr9_137023710_R GTCTCGTGGGCTCGGCGCTTGCATTAACAGCCCTC 59.9 0.03
mm4__chr7 51.32 Mbp_chr7_51317116_F TCGTCGGCAGCGTCCTCTCTGGGCGGAGTGGG 61.4 0.03
mm4__chr7 51.32 Mbp_chr7_51317116_R GTCTCGTGGGCTCGGTTCTCACCCTCCAACGTCAG 59.3 0.03
mm4__chr2 170.77 Mbp_chr2_170771334_F TCGTCGGCAGCGTCGCTCTACCGCACCTGGTTC 60.4 0.03
mm4__chr2 170.77 Mbp_chr2_170771334_R GTCTCGTGGGCTCGGGCTTCCTCACCTCTTCCGAC 60.1 0.03
mm3__chr20 3.82 Mbp_chr20_3820252_F TCGTCGGCAGCGTCCGGCCTTTTCCGCAGACT 60.3 0.03
mm3__chr20 3.82 Mbp_chr20_3820252_R GTCTCGTGGGCTCGGGTTGCCCTAGATCAGCGGAG 60.2 0.03
mm4__chr16 1.08 Mbp_chr16_1075756_F TCGTCGGCAGCGTCGCTGTCTCCAGACCACTGTC 60.0 0.02
mm4__chr16 1.08 Mbp_chr16_1075756_R GTCTCGTGGGCTCGGCCAGGGACCAGCATGGAAAT 60.0 0.02
mm4__chr1 107.14 Mbp_chr1_107141332_F Primer3: not found at this Tm N.d. 0.02
mm4__chr1 107.14 Mbp_chr1_107141332_R Primer3: not found at this Tm N.d. 0.02
mm4__chr19 42.44 Mbp_chr19_42440505_F Primer3: not found at this Tm N.d. 0.02
mm4__chr19 42.44 Mbp_chr19_42440505_R Primer3: not found at this Tm N.d. 0.02
mm4__chr22 38.93 Mbp_chr22_38927384_F TCGTCGGCAGCGTCGTGGGTGGGATTCAGGCTG 60.3 0.02
mm4__chr22 38.93 Mbp_chr22_38927384_R GTCTCGTGGGCTCGGGTGCTGCCATCTGCTCTGG 61.1 0.02
mm4__chr16 67.28 Mbp_chr16_67279445_F TCGTCGGCAGCGTCCCCGCACCTTGTCTTCCG 60.7 0.02
mm4__chr16 67.28 Mbp_chr16_67279445_R GTCTCGTGGGCTCGGCTGGGAAGGGAGGGCAGT 60.6 0.02
mm4__chr2 185.74 Mbp_chr2_185739016_F TCGTCGGCAGCGTCAGCAGTGCAGAGACGTGAG 59.7 0.02
mm4__chr2 185.74 Mbp_chr2_185739016_R GTCTCGTGGGCTCGGAAGAGGGCAGCCTCCTGA 59.8 0.02
mm4__chr20 3.22 Mbp_chr20_3218660_F Primer3: not found at this Tm N.d. 0.02
mm4__chr20 3.22 Mbp_chr20_3218660_R Primer3: not found at this Tm N.d. 0.02
mm3__chr11 46.39 Mbp_chr11_46392061_F TCGTCGGCAGCGTCTGTCCGCGTTGTCATGGG 60.3 0.02
mm3__chr11 46.39 Mbp_chr11_46392061_R GTCTCGTGGGCTCGGAGAGGCGCGAAAATAGCTCA 59.8 0.02
mm3__chr3 49.17 Mbp_chr3_49171178_F TCGTCGGCAGCGTCTTCCTGCTTTATGCCCAGGT 59.2 0.02
mm3__chr3 49.17 Mbp_chr3_49171178_R GTCTCGTGGGCTCGGCCCAATTACAGGCTTCCCGG 60.7 0.02
mm4__chr9 114.41 Mbp_chr9_114406690_F TCGTCGGCAGCGTCCAAGTCCTGTGCAGAGGAGG 60.0 0.02
mm4__chr9 114.41 Mbp_chr9_114406690_R GTCTCGTGGGCTCGGCACTGTCTTCTCGGCTCCAC 60.3 0.02
mm4__chr11 62.79 Mbp_chr11_62786683_F TCGTCGGCAGCGTCTTTCAAGAGTCGTCCTCCGG 59.3 0.02
mm4__chr11 62.79 Mbp_chr11_62786683_R GTCTCGTGGGCTCGGCGAAAGAGAAGGGTCCTCCG 59.8 0.02
mm3__chr16 180.40 Kbp_chr16_180401_F Primer3: not found at this Tm N.d. 0.02
mm3__chr16 180.40 Kbp_chr16_180401_R Primer3: not found at this Tm N.d. 0.02
mm4__chr10 104.64 Mbp_chr10_104641352_F Primer3: not found at this Tm N.d. 0.01
mm4__chr10 104.64 Mbp_chr10_104641352_R Primer3: not found at this Tm N.d. 0.01
mm4__chr15 82.09 Mbp_chr15_82092391_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 82.09 Mbp_chr15_82092391_R Primer3: not found at this Tm N.d. 0.01
mm4__chr15 63.94 Mbp_chr15_63941535_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 63.94 Mbp_chr15_63941535_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 120.64 Mbp_chr12_120640493_F Primer3: not found at this Tm N.d. 0.01
mm4__chr12 120.64 Mbp_chr12_120640493_R Primer3: not found at this Tm N.d. 0.01
mm4__chrX 153.64 Mbp_chrX_153644228_F Primer3: not found at this Tm N.d. 0.01
mm4__chrX 153.64 Mbp_chrX_153644228_R Primer3: not found at this Tm N.d. 0.01
mm4__chrX 149.47 Mbp_chrX_149471143_F TCGTCGGCAGCGTCTTCTCTGAGGAACCACACGC 59.9 0.01
mm4__chrX 149.47 Mbp_chrX_149471143_R GTCTCGTGGGCTCGGCCTTACAGCTCTGATCCGGC 60.2 0.01
mm4__chr14 55.27 Mbp_chr14_55271270_F TCGTCGGCAGCGTCCCTTTCCCTACAGTCGTGCT 59.3 0.01
mm4__chr14 55.27 Mbp_chr14_55271270_R GTCTCGTGGGCTCGGGCCACGGATCCAGAGTTTCA 60.0 0.01
mm4__chr22 50.19 Mbp_chr22_50185190_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22 50.19 Mbp_chr22_50185190_R Primer3: not found at this Tm N.d. 0.01
mm3__chr13 110.21 Mbp_chr13_110205352_F TCGTCGGCAGCGTCGCTCACAATAGTGCCAGCGT 61.0 0.01
mm3__chr13 110.21 Mbp_chr13_110205352_R GTCTCGTGGGCTCGGGCCTCTGACGGTTACTGCTT 60.0 0.01
mm3__chr10 48.66 Mbp_chr10_48656424_F TCGTCGGCAGCGTCGACTTCCTGGGCCCTGGT 60.9 0.01
mm3__chr10 48.66 Mbp_chr10_48656424_R GTCTCGTGGGCTCGGGGCGAGATGAGGTTGGGTAA 59.4 0.01
mm4__chr16 67.16 Mbp_chr16_67164699_F TCGTCGGCAGCGTCCCATCCAGCCAGCCTTTTCT 60.3 0.01
mm4__chr16 67.16 Mbp_chr16_67164699_R GTCTCGTGGGCTCGGGGAAAGTGCTGCGTTTGCC 60.6 0.01
mm4__chr13 30.12 Mbp_chr13_30115165_F TCGTCGGCAGCGTCCTGCGTCCCCAGACAGAAAC 60.9 0.00
mm4__chr13 30.12 Mbp_chr13_30115165_R GTCTCGTGGGCTCGGGGGAAAATGCGTCCACTGTT 59.0 0.00
mm4__chr3 50.23 Mbp_chr3_50227487_F TCGTCGGCAGCGTCAGGTGCTAGGTCGGCCTC 60.7 0.00
mm4__chr3 50.23 Mbp_chr3_50227487_R GTCTCGTGGGCTCGGCTCTTCCGGGGCCTCTCTA 59.7 0.00
mm4__chr2 233.03 Mbp_chr2_233032104_F TCGTCGGCAGCGTCGCCCCTGTCTCTTCCTTTCC 60.0 0.00
mm4__chr2 233.03 Mbp_chr2_233032104_R GTCTCGTGGGCTCGGGCTTCTAAGGCTCTGCTCCC 60.1 0.00
mm4__chr10 48.31 Mbp_chr10_48307205_F TCGTCGGCAGCGTCGAAACACTTCGGCAAGGGTC 59.4 0.00
mm4__chr10 48.31 Mbp_chr10_48307205_R GTCTCGTGGGCTCGGAAATACGGTCCCTATGGGCG 59.6 0.00
mm4__chr13 33.35 Mbp_chr13_33350215_F TCGTCGGCAGCGTCAAACTCATGCCCTGGAGCC 60.0 0.00
mm4__chr13 33.35 Mbp_chr13_33350215_R GTCTCGTGGGCTCGGGTGGAAGAACGTCCGGGAG 60.0 0.00
mm4__chr1 50.33 Mbp_chr1_50333166_F TCGTCGGCAGCGTCGTCCACACTGTCCTTGGGAG 59.9 0.00
mm4__chr1 50.33 Mbp_chr1_50333166_R GTCTCGTGGGCTCGGCTTTCCCGGAGCACTCGC 60.8 0.00
mm4__chr22 37.20 Mbp_chr22_37199618_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 37.20 Mbp_chr22_37199618_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 152.44 Mbp_chr7_152435511_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 152.44 Mbp_chr7_152435511_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 74.78 Mbp_chr15_74781988_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 74.78 Mbp_chr15_74781988_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 19.10 Mbp_chr9_19102920_F TCGTCGGCAGCGTCCGCCCTTAAGGAAGAGCAGT 59.7 0.00
mm4__chr9 19.10 Mbp_chr9_19102920_R GTCTCGTGGGCTCGGCGGAAGGGAGGAGAGAAGGA 60.0 0.00
mm4__chr9 134.82 Mbp_chr9_134818695_F TCGTCGGCAGCGTCAGAGCTCCGGACCTCATTCT 60.0 0.00
mm4__chr9 134.82 Mbp_chr9_134818695_R GTCTCGTGGGCTCGGGATCCCTCGAAGGCCATCC 59.6 0.00
mm4__chr8 96.15 Mbp_chr8_96145306_F TCGTCGGCAGCGTCAGCTCCAAGCACAGCTGC 60.6 0.00
mm4__chr8 96.15 Mbp_chr8_96145306_R GTCTCGTGGGCTCGGAGCTCTTCCCTTGCCTTTCG 60.3 0.00
mm4__chr7 77.70 Mbp_chr7_77697100_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 77.70 Mbp_chr7_77697100_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 27.50 Mbp_chr3_27496242_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 27.50 Mbp_chr3_27496242_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 46.81 Mbp_chr20_46811116_F TCGTCGGCAGCGTCGCCACCGTCTTCTCCAATGA 60.0 0.00
mm4__chr20 46.81 Mbp_chr20_46811116_R GTCTCGTGGGCTCGGCTAACCCCTTTGTCGCACCC 60.9 0.00
mm4__chr2 226.84 Mbp_chr2_226836201_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 226.84 Mbp_chr2_226836201_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 190.37 Mbp_chr2_190372561_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 190.37 Mbp_chr2_190372561_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 105.24 Mbp_chr2_105235807_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 105.24 Mbp_chr2_105235807_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 40.60 Mbp_chr19_40602274_F TCGTCGGCAGCGTCACGTGCGACAGAAATCCAGA 59.6 0.00
mm4__chr19 40.60 Mbp_chr19_40602274_R GTCTCGTGGGCTCGGTCCCCATCTCTAGGAGTGCC 60.1 0.00
mm4__chr19 13.85 Mbp_chr19_13850682_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 13.85 Mbp_chr19_13850682_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 82.96 Mbp_chr17_82960836_F TCGTCGGCAGCGTCGCGCGTGGTCATTTTACCTC 59.9 0.00
mm4__chr17 82.96 Mbp_chr17_82960836_R GTCTCGTGGGCTCGGCGTCCACGAAGGGGCATTT 60.6 0.00
mm4__chr17 145.03 Kbp_chr17_145031_F TCGTCGGCAGCGTCGCTTAACTAGACACCAGGCCT 59.4 0.00
mm4__chr17 145.03 Kbp_chr17_145031_R GTCTCGTGGGCTCGGGGCAAGGGCTCCTCTTTTCT 59.9 0.00
mm4__chr14 103.19 Mbp_chr14_103189089_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 103.19 Mbp_chr14_103189089_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 52.45 Mbp_chr13_52450652_F TCGTCGGCAGCGTCTCCAAACGAAGCGGTCCATG 60.9 0.00
mm4__chr13 52.45 Mbp_chr13_52450652_R GTCTCGTGGGCTCGGCTCCCTCCGTCCCTCACC 60.7 0.00
mm4__chr11 69.01 Mbp_chr11_69013743_F TCGTCGGCAGCGTCGAACTGGCAGAGGTGGTCAG 60.3 0.00
mm4__chr11 69.01 Mbp_chr11_69013743_R GTCTCGTGGGCTCGGGGGCTGGGGAAAAGGGAATT 60.2 0.00
mm4__chr10 1.51 Mbp_chr10_1514587_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 1.51 Mbp_chr10_1514587_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 966.55 Kbp_chr1_966552_F TCGTCGGCAGCGTCCTGTACGACTCTGGCCATGG 60.1 0.00
mm4__chr1 966.55 Kbp_chr1_966552_R GTCTCGTGGGCTCGGACCTGTTTCCCTTCAGCGAG 59.9 0.00
mm4__chr1 4.16 Mbp_chr1_4161784_F TCGTCGGCAGCGTCCCACCACCACCAAACCTCAT 60.1 0.00
mm4__chr1 4.16 Mbp_chr1_4161784_R GTCTCGTGGGCTCGGGTGTGCCCTGACCCATCTAC 60.1 0.00
mm4__chr1 143.72 Mbp_chr1_143717610_F TCGTCGGCAGCGTCCTTGCCCCTAGCCTGCTTTA 59.7 0.00
mm4__chr1 143.72 Mbp_chr1_143717610_R GTCTCGTGGGCTCGGAAGAACGGGAAGAGCCATCC 59.7 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529732 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr5 134.58 Mbp_chr5_134577956 GCTGGGAGGCAACGCTTATAAAGACTGAGCGCCCTCTGGCGGCCTGAGGGCATTTTCCTCCAGATTCCTCCTTCCCCAAC
CC
mm2__chr19 18.39 Mbp_chr19_18388563 ACTGCTGGCAGAATCTTCGTCCGCACGGCCCCAGCTGGAGTTGCACTTGCGGCCGCAAGCCGCCAGGGGGCGCCGCAGAG
CGCGTGCGCGCAACGGGGACCACTGTC
mm4__chr9 16.42 Mbp_chr9_16419240 CCACCATTGCTCCCAGACAAGCTGCTGAACATAAGAGATCCTGAAACTTCAGCCCCTAGGCTGCCAGGGAGGGCAGGGGC
CTCGGCCTTGTGTGCCGACTCCCCACTGTCACTCGCCCCGTCAATGTCATCGAGA
mm4__chr1 44.02 Mbp_chr1_44020815 TATGAGAACGCGTCAGGGTGGGGCAGCCCCTGGCGGCCTGTGGTGCTTGCAAGGCCTCCTTGGATTTGGACA
mm3__chr9 136.36 Mbp_chr9_136363835 TTCTTGGGCTTCCGTCTTCCGGGTCGGGGGCGCGCTGCGCTATGCCGCCCCCTGGCGGCCTGAGGGGGCCCTGCGTCCAC
GTGCAGCTCGCACCCCGAGGACAC
mm4__chr8 106.82 Mbp_chr8_106817530 GCTTTGGCATGTGGGTGATGTCAGAAATATGCTGGTTTACAAATGCCAACACCCAAGGCCACCAAGGGGCGCAGAATTCA
CCTGCCAGAGTGTTTGACACTTGTGTCAGGAAAAGGCAACAGCTGTGAAAAGC
mm4__chr10 80.17 Mbp_chr10_80169256 CATAGGGAGGAACAGGCTGCTGGGCAGAGGGGGGCTGCCCAAAGCCGCCAGGGGGCACTGGTGGGTAGCCAGCCCCAGGG
GCCCCAGGGTACAGGTTGGGCATGTT
mm4__chr1 27.55 Mbp_chr1_27552129 AGGTAGTCAGGAGAGCTGCACACGGCACTGGAAGTCACCACCAGGCCCTGGGGCTTCACACGCATCCTGACCTTGTCCTC
CGCAGGCCGCCGGGCGGGGCCGGGGCTGACATGAGGGTGCGAGAA
mm3__chr1 110.62 Mbp_chr1_110619505 AAAAGACAGACGCACGAGCTGCCACTGGCCGCCAGGGGGCGCCACATGCAGCGGTATCTGCACACTGTGGCT
mm4__chr2 121.07 Mbp_chr2_121071185 CCTGGTGAAAGAAGGGCAGTGGTGCCCGGCGCCGACTGCCAGCCTAGGGCGGACGGCAGGAGGGGTCCCAGTCCCAGCAC
CACCTCCGCTCCCCTCAGGGCTTCCCCAAGACAG
mm4__chr11 120.51 Mbp_chr11_120511226 TGAGGCCGCAGTCTCTTCTAACGGGGCACGCTCGTCCCCCGCGCTGTTTCCCGAATGCCACCAGGGGGCGCCCCTTGCTC
GCTGAAGCCGCCGGGCTAATAACTCCTCGGGGC
mm4__chr19 43.60 Mbp_chr19_43598824 AGGTCTTGGAGACTCCTGCTGGCCTCCCCTGACCTCTCCCCCACATTCCTCAGGCCACCTGGGGGCCCCGAAGTGCACCC
GCTGCCTCATCACCTTCGCAGATTCCAAGTTCCAGGAGCGTCACATG
mm4__chr11 65.00 Mbp_chr11_64996960 ACAGAGGTGCATGGCTTAGGCGGGGGAGCAGAGTGGTCCCTCAGCAGCTGTGACTTCCCAGTGCCCTCTGGAGGCCTAAG
GAAGTGGCAGCTCTTTAAATCCCCAGTGGCCACTCCGCCCCCAGAGGGCCAAGTTTCAGT
mm2__chr3 50.26 Mbp_chr3_50260133 CTCGACTGGGTGGGGCTCCGGCGTTGGCGCATCCGCCTCCGGCCGCCAGGGGGCGCCGCCGCCCGTGCTCTGCCCTGCCT
GGCTGGTCACAGAGGCTGACGTCAG
mm4__chr17 46.89 Mbp_chr17_46891086 AAGCACCTGATGGGGAGAGACAGGAGGGCGACCCCTGGCAGCCTCAGGGCTGTCTGGCTAGTCTCAGCCT
mm3__chr15 68.88 Mbp_chr15_68876010 AGTCCATGTGTAGCTGCAAGTAGCTTTCTGGAACTATTGGTGTTGTTTGCGCGCCCCCTGGCGGCCAATGGCCTTCCTTG
TCAAGGAGGTTGGCACAGA
mm4__chr3 51.94 Mbp_chr3_51942210 AAGTCGGAGCAAGAGCCTTCTCTGTGTTTGCGCCCCTTGGCCGCTGGGGGGCGCCAGAGACGCGGCGCCCTTTCGCGTTC
TGGAACGTGACTCCTCCC
mm3__chr4 674.38 Kbp_chr4_674378 CACAAGCCAGCAAAGCCTTGGAGGCAAAGGCGGAGCTGGGCGCACGCATGCGCCGTCAGCGGCGAGAGAGCGGGGGGCCG
CGCCCCCTGGCGACCGAAGGGTGACTGCGCGCCCCCGCGCGGCGGTGACGTCACGTGAGG
mm4__chr14 104.56 Mbp_chr14_104559907 ACCAGAGAAGTCCCTCCCTCCTCTTCCCCAAGACTTGACAGCCGGGCTGTGCCCTGAACCCGCCCCTACTCCCCCTAGGC
TGCCTGGGCGCCCCGCATTCACTGTTTCCACTCTTGACAGATGGGGAAGGGG
mm4__chr16 1.90 Mbp_chr16_1900522 TGCTCTTCTCCAGAACGCAGCCCCGGCGGCAACCCCAGGCGGCCGAAGGGACATGGCAGCAGCAGGCTGGGCTGACCACA
AATATTTGCACAGGTGAACACTTTAGAAAAGGAAGTCAAATGTAGCATGCGGGTGG
mm4__chr13 80.34 Mbp_chr13_80343017 GAATCCATTGAGCCGACGGTGAACGCAGGCGCCGCTGCGCTCGCGGCCCCTCTGGCCACCAGGGGGCGCCCGCCCGGCGC
GGGGCTAAGGCTGCTTTCTTCTCCGGGACAC
mm4__chr16 10.26 Mbp_chr16_10256972 AAGGGGAAGGACTTTGTGGGGTATCTGAGGAAGATGGAGTTTCTCCTGAGGCCACCAGGAGGCGCCAGACGTCAAGCGGG
GCGGGGGGCGGGGACGGGCTCGGTAACTTTCCTC
mm4__chr17 9.00 Mbp_chr17_8998194 CAGCATTTACTGTGCGAGCCACCTGGGGGCCATTTTTGTTGCCCGAGGTGGTGCGCCTGCGCCACCTGGTGGACTACGGG
AAAGTAGCAGATCGGTGAGATGGGTTGAGGCACCTTCA
mm4__chr1 3.50 Mbp_chr1_3502551 GTGGGATGGGGCTAGAATGGGGAGGGCAGGGGGCCTCCTGGAGGCCTAGGGTCCCCCCTGCTCCACCCGGCAGGTGAATG
AGACCCCAGG
mm4__chr14 100.24 Mbp_chr14_100240416 CAGGGATTGCTGCCTCCGGGACGCGCGCCCCCGCCCCCGCCCGGCGCTGCCGTTACCCCATCGGCCGCTGGAGGGCGCCG
CGGGCGCTTGCCCCGCCCGCCCCCAACTTCCCCGGACTCCCAA
mm4__chr19 55.09 Mbp_chr19_55092816 TCCACTCCTTTCCGACCTTGCGGTGCTCCCTGGCCGCCTGCGGTCCCGGACCCTGCCCGTCGGGCGCCTCTGCTGGGGGA
GGGGCAGGAATCAGCCCAGGCACCTC
mm3__chr12 53.32 Mbp_chr12_53321676 AAAGAAGGACGAGTACGCGGTCCCGGGACAGACCTCCAAAGCTTCTTCCTGCGCCCCCTGGCGGCCGGAGGGGGAGCAGC
GCGGACACGTCCCCCGGCCAACCGTTGGTTACCAGGCTGGAGTG
mm4__chr19 38.40 Mbp_chr19_38403115 GGGAAGATGCCGATTGGTCAGTGCCGCCGGACGCTGCGGGGAGCTCGTGCGCGCGGCGCTTTTGGGGCGGAGCCTGCCAC
CGGCCGCCAGGGGGCGCGCCGAGACCACACTTTCATGGAGGCG
mm4__chr12 76.08 Mbp_chr12_76084302 GGAAAAGACGGTGGAGCTCAGAGAACCTTAGGCCTCCAGGCCGCGCCAACCCCTACGTCGCCTCAGCCGTCCCCGATAAA
AGCGCTGGTCGGC
mm3__chr1 112.72 Mbp_chr1_112718842 GACCTCCTCTGAGTTGCCACCACGGAGGTGTCCATCCTCACTGAACCTGCGGGTGCCGGATGTCCTCTCTCCCTTCCTCT
TGCCCTCGGCCGCCAGGGGCCGCTGCGTCTCGGCTGTGCCCATCCGTTCAGCCGAG
mm3__chr9 127.79 Mbp_chr9_127785126 CTTGCTCGTCGCCGCTAGTGCCGCCGCGCCCCCTGGCGGCCGTCGGTTGCCATGTCAACGGAGNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNCGTTCTCTCCTCCCAGGGCC
mm3__chr1 8.85 Mbp_chr1_8849074 GAGGCCCAGGGTCTTGGGGCAGCCCCACTGCGCCCCCTGGCGGCTGAAGGAAGCTCCTGGGCGCGTCTGGACCTGGACCA
CCAGCTGACCTTCTGCACAGT
mm4__chr12 576.27 Kbp_chr12_576266 CTAAAGCTGCAGCACCGAGAGGCCACGTGGGGCGAGGCCTCCCCGGAAGGCCTGCGGCGCCGCCGTTGTCCACCAGGGGG
CGCCAGGAAACGGCGCGGCGAGGCCCCAGCTCAACCC
mm4__chr22 42.61 Mbp_chr22_42614906 GAAAGCAGTCGAGACCCCGCGAGCCCGCCCCTTCGGCCGCTAGGGAGCGACGAACTACTTCCGGCGTCCGCGGACCAACT
CTCGCGACAGCCAGCTCAAAGCAGGCAAGAACCG
mm4__chr7 100.69 Mbp_chr7_100693678 GGAGAACTCCGGGAAGCGCGGGGCCCGGCCACCCTAGGACGCTAGGGTCGGGGGCCCCCAGGCGGCCCAGGGCAGGGCTT
GGCAGGGGCCCGGGGGCGACCGTTAGGAGC
mm4__chrX 110.17 Mbp_chrX_110168060 CTGTTGGCTCAGTCACTGCTGCCCATCTGCCACTAGGGGGCGCCGAGCAGAGAGGATGGAGGGAG
mm2__chr11 61.76 Mbp_chr11_61758136 GTGGGGAGTACAGTTGGGTCCGGAGCCCCCTGCCGGCCTAGAGTATGGAGGGGGCTCCCGGACATTCTGTCCTCCCTGTG
CCCCAGGTCGCATCAGTGAGGGAAGT
mm4__chr2 71.94 Mbp_chr2_71944374 GGCTCAGACAAGCAGGGATTCTTCCCAGCGCCCCCGGGAGGCCTGAGGTCCTGCCTCCTGCCTGGGTGGGCATCAGGAAA
GCTCAG
mm3__chr13 113.39 Mbp_chr13_113394138 TTCCTCTTGCAGCAGCCGCCGCCCGGGAGGCCGGCAGCGCCCCCTGGCGGACTCAGGCAGGAGGCGTCCNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCCCGAGGCCTGTCCCTGCAGG
mm4__chr11 112.28 Mbp_chr11_112280090 GCACTTGGCTCTCAGGGATTGGCTCTGAAACCTCAGTGGTAAGGCGTCTTCCAGAGCAGCGGCGCCCCCTGGTGGCACGC
GGTAGGATGGGCGGCAAAGAAG
mm3__chr4 8.47 Mbp_chr4_8473264 CCTGAGCATGACCCATGAGGGCCACAAGGGCCAGCCCTGCCTCTGTCCCTGCCCCTTCCTGAGGCCGCCAAGGGGCGGCG
CGCTGCATCCATGCTGGCTGGTATGG
mm4__chr6 64.30 Mbp_chr6_64300256 GCTTGGTAGTGGGGAAGCTCATGACACCCCAGGCCGACAGGGGCCGCGGCAGGGGGCAGAAGCAACACAGACAAAAAGCA
GCACCTAGGCAAGT
mm4__chr1 225.48 Mbp_chr1_225475082 AATCCCAAACTGAGAGGCCGGGAAAAGGCAAGGACGCCCGTCGGCCGGCAGAGGGCGCTGCGCACCCGCGTTGGACGGGG
CGCGCTGCCGAGCATGACCGCCAGGGGTCG
mm4__chr16 33.84 Mbp_chr16_33844703 ATGACAGAAAGCCAGCCCTCAGCAGCACCTGCACCTGCCCTGGAGACAGCCCCGTGCACAGTGTCCTGGCGCCCCCTGGT
GGTCTCTGGGACCCTTTCAGGGAGGTTTGTGCCTG
mm4__chr16 33.01 Mbp_chr16_33009677 GAAGTCAGTGAGAGCCCAGGCACAAACCTCCCTGAAGGGGTCCCAGAGACCACCAGGGGGCGCCAGGACACTGTGCACGG
GGCTGTCTCCAGGGCAGGTGCAGGTGCTGCTGAGGGCTGGCTTTCTGTCAT
mm4__chr20 3.71 Mbp_chr20_3711063 CAACTTGGGTTGCAGCTGTGGCCTAGTGAGCACGCAGCGCCCCCTGGTGGTCGAGGGGGAATTGCGGGTCCCGGGAAAGG
GGGCGGTGTGCCAGCAACAGGGAGCAGGCAGCTCTGCAGCCCTGAACCATCCCTCCCTTG
mm4__chr22 41.92 Mbp_chr22_41919574 GGCAACTCTGACTCAGGCTCCAGCTGCCCGTGGGAGGGAGGGGGCGCCCGGGCTCCTGAGGTCGCCAGGGAGCGGCGGGA
CTGGGAGGCTCCAAAGCCCTCAGTGTACGT
mm4__chr11 133.95 Mbp_chr11_133946643 AGGACATGGAGGTGAGGGGCCCGGCCCCCTCTGGAGGCCCACGGCACTGCCCAGTTGTGGCCGCTCGTGCACAGGGAACA
TTCTTTCTGCTCCAGGACGCCTGCCACTACAGCCTGGTGGGAAATGACAGAGCCAGCCT
mm4__chr12 120.58 Mbp_chr12_120583559 CACTCCCCACCCAAGAACAACTTTTCACCAGAAAGTGTCTGCGACATCCTCCTTTGGCCAACAGGGGGCGCCAGAGGTCC
GCAGTGCGCGGAGAGAGCAAGACACCC
mm4__chr11 6.26 Mbp_chr11_6259986 CTAAAGCTGAACCGGAGCGTGCAGGGAACCGGACCCGGGCCGGGGGCTTCCCTGTGCCGCCCGGGGGCGCCTCTCCTCAA
CAGCAGCAGTGT
mm4__chr9 137.24 Mbp_chr9_137236107 CTACCTGCTGCTCGGATTCCTGCTGCTGCCCCTGGCGGCCTTCGGGCTCTCCCTGGCAGGGGGCATGGAGCTGGCCGCTG
TCGGGGGTCCCCTGGTGGGGCTGGTGCTCCTCGTCATCCTGGTTACTGTCCTGCAG
mm3__chr7 78.04 Mbp_chr7_78040366 CAAGCAGAGCGAGGATGAGGGGAGGCACAGGCGGGAAGGAGGACGGGCGCCCCCTGGCGGCCCCTGGTCGTGCCGCAGCC
TCACATTCCCGCGGGTTCCCTTCTCGCCGCGTTCTCTAC
mm4__chr1 244.37 Mbp_chr1_244367647 CAGAGAGCAGAAGACAGCCCAGCCTTAGGCCTCCAGGGGCCCCAGGGCCTGCTGTGCTCAGTTCCACCCAAACCCCCGGA
CTCCCGGCCAGGTCCAGACACCACAGAGCACTAGGCAGGAG
mm4__chr5 7.87 Mbp_chr5_7869332 GCCCTGCACTAATCTCCTGGGAGAGGCGGCCCGGGGCGGGGGTGGGCAGCCGGCTTGCGCCCGTGGTTCCCACGCCCTTC
GGCCTCCGGGGGTCGCCGCGGGCGCGGGCTGGGGCTCGGACTTGGCCTTT
mm3__chr3 3.18 Mbp_chr3_3179778 GGTGCCGAAGACTCCGAGGGAGGGCCCGTGTCGCGCGGCTGCCGCGCACCGGGGCCACAGCGCCCCCTGGCGGCCCCAGG
GGAGCAGACGGCGGCGCGTACCCAAGCCTGAAAGTGATGGCCT
mm4__chr17 31.53 Mbp_chr17_31525159 CTGTCCTCAGGGTGCATGAGCTGGAGGAGATGGTGAAGGATCAGGAGACCACGGCCGAGCAGGCTCTGGAGGAGGAGGCG
CGGCGCCACCGCGAGGCCTACGGCAAGCTGGAGAGGGAGAAGGCTACC
mm4__chr8 22.55 Mbp_chr8_22551974 TTCTCCTTGCCCTTCCTCCTCGAGCGCCCGCGCCAGGCAGCAGCCGGGCAGGGATGCTCCTGCGCTCCCGGGCGGCCTCG
GGCCCAGCCACCTGCTCGCCGGGGAAGGTAGGTCCGGGCAAG
mm4__chr1 46.53 Mbp_chr1_46532983 AATGGCACTGAAGGAAGGGGAATGAACCCGGACAGCGGAGTCACGGCGGGGCCCTGGTCCGCTAGGGGGCGGCGCGCGCC
CGGGGTGGGTTGAGCTGGACTCCTTC
mm4__chr3 128.61 Mbp_chr3_128608519 GACACACAGCCCAGTAGTGGCGGGCGGGCGCCCAGGCTGCCACCGCGCTGAGGACCGGCCCCTCTGCCAGCCGGGAGGTG
CGCGTGGCCTAGGCGGGGGCGCCCTCTGGCGGCCGCGGGTTCCAATCCCTGCCGGCCTC
mm4__chr17 7.01 Mbp_chr17_7014897 GTCAGCGTCCACAAAGGTTCGACCTCCCTCGCGGACAGCGCTCCCTGGCGGCCACGGGAGGGCGGGCCCCCCACTTGGCT
CGCGGCTCTTC
mm4__chr8 123.16 Mbp_chr8_123160593 CTGGGCTGATCCTGCCTTGGGCCCACTCTCCCGGCGACCTCCCGGCTCCCAGACCTGGCTGAGCCCGGCGGCCGCCAGGG
GACGCTGTGCCCAGGGGTTGCCGCCGCTGCCCCGCAGGTTTCGGCTTTT
mm4__chr9 69.04 Mbp_chr9_69035712 CCCCACAGAAGAGTGCCTGCGGCCAGTGGCCACCAGGGGTCGCCGCAGCACCCAGCGCTGGAGGGCGGAGCGGGCGGCAG
ACCCGGAGCAGCATGTGGACTCTC
mm4__chr17 28.35 Mbp_chr17_28347146 GGCTGAGTTCCTGTGCTTCTGTTTATCATCTGACCTTGTGTTCTAGCCAGGTCGTAAGCGCGACTGTACTGTTGCCCTGC
AACCCCAGCTCCCCCTGGAGGACTAAGGACAAGAATAACAAGGGCTGGAACTGTGA
mm3__chr14 69.73 Mbp_chr14_69726928 CTGCAGCAGAGGCCGGAGGAAGCGCCACGGGCGGCTCAGACCTGAGGCCGCAAGGGCGCGCCGGGCGGGCAGGGCGGAGG
GTCCGATTTCCTCCCTCTGAACGGAAAAAAAGCCACTTTCCCTGGGAAGAA
mm4__chr11 76.21 Mbp_chr11_76208948 CACCTTCCGCCTGAGCTCGCGGGTCAGGGTGCACGCGCCTCCTGAAGCCCCCAGCCCGCCAGGGGGCGCTAAGCCGGGGC
TCGAGGAGGGGGGAATCGCGGCTGCCTCCTGCGTCGCGGGCTGGGGTCAAGTCCTCTTC
mm4__chr12 121.50 Mbp_chr12_121500058 TACCTCCCGTTTCCCTCTGTGTGTGGCCCCCCTGCGGCCACCAGGGGGCGCCCTGGGCTCTGTCCCTCAGGGAAGAATTT
TCATTTGTGTATGACAGGCTCCGGGAAAAG
mm4__chr1 112.28 Mbp_chr1_112283901 GAGTGGTCTTGGTCTCAGGGGGCGTAAGGCTCCTCCTGGCTGCCTAAGGAAATTATTCATTCGGATAAACCCAAATTAGA
CTACAAAAAGTAATAGCACATTTCTCTTTGTCTTCCTGATGTCTGGAGAG
mm4__chr16 66.27 Mbp_chr16_66270596 CAGGGTCCGGGTTCGCTGGGAGGCCGGCGGCGGCCGCGTGGCCACAGCGCCCCCTGGCGGCTACGGGAGTCCTGCACGCA
GCGGCCGCGTCTCCACGCCTTCCTGGAGCAGAAGGTT
mm4__chr11 47.19 Mbp_chr11_47186120 CCTGAAGACGGCTCAGCCCTCCAGCGCCGGCGCCGCCTCCCCCGGGCGGCCTGCGGAATTGCGAAGCCCGCGGCACCGCA
GATGGGACGGCCCCTCGGCGCGGCTACGGCCCTTCCCGTC
mm4__chr12 13.28 Mbp_chr12_13278332 TTGAAGCTCCGCAGAGAGTGCCTCCAGCGGCCGCCAGGGGTCGCAGTCGAGCAGCTGGCGCCGCCTCTGTGCAGGGGAGC
AGGGGCCGCTGGCCGGGAATCGCAAGCCGGTCTCCTGTACAT
mm3__chr1 40.37 Mbp_chr1_40373901 CCCGCAGCCTCTCTTGGAGGCGGGCCTGTCCCTAAGCCCGCCAAGGGGCGCCGCGCCGAGGGGCTGCGGAGTGGGGGACG
GACGCCCCCGACCCGGGAAGGGGCGTCCGGCGGGGCCGGAGGAGAGGGCTCT
mm4__chr9 137.02 Mbp_chr9_137023710 ACCAGCTGTGCCTTTAGGTGGCCGGGCCTTTCACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGC
CAGCTGTTCCTGAGGCCGGCAGGGGGCCCGGGAGGGCTGTTAATGCAAGCG
mm4__chr7 51.32 Mbp_chr7_51317116 CTCTCTGGGCGGAGTGGGGGATCCCGGGGCCGGCGCCTCCTGGCGGAGTTGGGGGAGGGGAACCACCTGGAGCCTGGAGG
CCGTGCGAGGGCGAGGTGGGCGGACCAGCCCGGGAGGACTGACGTTGGAGGGTGAGAA
mm4__chr2 170.77 Mbp_chr2_170771334 GCTCTACCGCACCTGGTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGGAGGATGCGCGCCA
CTGGGGTGCGCCTGGCTTGCGGCACCCTCCTCCCGCAGTGGTCGGAAGAGGTGAGGAAGC
mm3__chr20 3.82 Mbp_chr20_3820252 CGGCCTTTTCCGCAGACTCCGCGCTCCGAGCCGCGGCGCCCCCTCGCGGCCGCCGGGGTCGGCAGCCTGGGGCTCCGCTG
ATCTAGGGCAAC
mm4__chr16 1.08 Mbp_chr16_1075756 GCTGTCTCCAGACCACTGTCCTTCCGGAATGCATGCTCCACAGGCAGCCAGAGGGAGCCGCAAACATGGCGTGTTGGATT
TCCATGCTGGTCCCTGG
mm4__chr22 38.93 Mbp_chr22_38927384 GTGGGTGGGATTCAGGCTGCCGGGGGCGGGGGGGGGGGTTGCCGCAGAGGGGGCTGGGGAGGGCGGCACCCCCCCATTGC
CCGTCCTGGGCCCTGCGTCCCCTGGCGGGCTGCGGCCAGAGCAGATGGCAGCAC
mm4__chr16 67.28 Mbp_chr16_67279445 CCCGCACCTTGTCTTCCGCGCCGGCAGGGCTGTGCCCCGTCGAGACCTGCTCGGGCTGTGGCTCCGTCCTCCGGCCTCCA
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCCCTCCCTTCCCAG
mm4__chr2 185.74 Mbp_chr2_185739016 AGCAGTGCAGAGACGTGAGTGGCCGCAAGCTGGGCGGCGTCGCCCTCTGGCGGCCGCAGGCCTGCTGGGGAGCGGGGCAG
GGATCGCCACACACGGGTCGCGAGGCTCCCAACTGTCCCCCGTCAGGAGGCTGCCCTCTT
mm3__chr11 46.39 Mbp_chr11_46392061 TGTCCGCGTTGTCATGGGGACGCGGCGCCCCCTCGCGGGCTGGGGAAGGAGCTGGCAGCTGGGTCCGGCCGCGCTCCCGC
CTCCGCCCTGACCATACTCCCGGGACCCTGAGCTATTTTCGCGCCTCT
mm3__chr3 49.17 Mbp_chr3_49171178 TTCCTGCTTTATGCCCAGGTAAGGGCGCCCCAGGCTGCCCCCACACGACAGAGGCCCAGGCCATGTCCCCAGTGGCCTCC
AGGGGGCGCCGCCGGGAAGCCTGTAATTGGG
mm4__chr9 114.41 Mbp_chr9_114406690 CAAGTCCTGTGCAGAGGAGGTCCCTGGGGTGGGTGCGGTGCCCGCTGGCGGGCTGCGGTTCTGTGGAGCCGAGAAGACAG
TG
mm4__chr11 62.79 Mbp_chr11_62786683 TTTCAAGAGTCGTCCTCCGGGGGCGGTGCAGAACCCAGCTTTGGGTCCGGCCTCCCGCTGCCGCCAGGGGGCGCGGGGCC
GGAGGACCCTTCTCTTTCG
mm4__chrX 149.47 Mbp_chrX_149471143 TTCTCTGAGGAACCACACGCTGTCTCCACCGCCAGGCTCCAGCTTGTCCCTAAAGCCAGGGCGGAAGGCGGCGGCGCCCT
GTGGCTGCCCAGGGGCCTGGGCTGTGTGGCCGGATCAGAGCTGTAAGG
mm4__chr14 55.27 Mbp_chr14_55271270 CCTTTCCCTACAGTCGTGCTCAAACTTTCATTCTCTACCACTTGGGGGCCGCAGACGCGTCATCCAGGTCTCCCTTTTCA
CTTCCCCCTTCCCGCCCCCAGTGTCCGGCAGGGGGCGCCGTGAAACTCTGGATCCGTGGC
mm3__chr13 110.21 Mbp_chr13_110205352 GCTCACAATAGTGCCAGCGTTTACCTGCGGGCCCTGGCGGCCTATGAGTCCTGGGTACCCGGGTTCACCAGGAAAACCCT
GAAACCGAAGAGAGAAGCAGTAACCGTCAGAGGC
mm3__chr10 48.66 Mbp_chr10_48656424 GACTTCCTGGGCCCTGGTGCACGGCGCCCCCTGGCGGGCCTCGGGGAGGCGGCCTCCTGCGTGCCCGGACCGCTCGCTGC
GCCTTCCCGCCTGGCTTCCGGTTACCCAACCTCATCTCGCC
mm4__chr16 67.16 Mbp_chr16_67164699 CCATCCAGCCAGCCTTTTCTGCCTGCTGGTGCCTCGGCCGCTGTCCGAGCCCCGCCCCGCGGGCTTGCACGTGGCCCCCG
CCTGACCCGGCGCCCCGGGGCGGAGTAGGGCGGAGCGGGCGGCAAACGCAGCACTTTCC
mm4__chr13 30.12 Mbp_chr13_30115165 CTGCGTCCCCAGACAGAAACCACGGCTCCCCCTGGCGGACACCGGTTCCCCAGCGCTGGGCTTTCCCCTTCAGTAGTTTA
GGGGTAGTTTTCAGCTAAAGAAAACAGTGGACGCATTTTCCC
mm4__chr3 50.23 Mbp_chr3_50227487 AGGTGCTAGGTCGGCCTCGGCTCCCGCGCCGCACCCCAGGCCCGCCAGGAGGCGCCGCTGCCCCGCGAAGCCGTTCCGGC
CTCTCCCGGCCACCGAAATCCCGCGCTCCGGCCTAGAGAGGCCCCGGAAGAG
mm4__chr2 233.03 Mbp_chr2_233032104 GCCCCTGTCTCTTCCTTTCCCACTGCAGCGCCGCCCCCTCGCTGCCTCAGGGGGAGCCCACGCAGCAGCGGGGGCAGACG
GGCCTCAGCCTCAGGCAGCCTGGGGAGCAGAGCCTTAGAAGC
mm4__chr10 48.31 Mbp_chr10_48307205 GAAACACTTCGGCAAGGGTCGGACGGAGGTGTCTTTGCGGCCCGCCGCGCCCTGGCTGCCTCCGGGGCGGCGGGGAGCCG
GGGAGAGCGCTTCTGCCGGCAGGTCCCCAAGCCTGCCCCGCGCCCATAGGGACCGTATTT
mm4__chr13 33.35 Mbp_chr13_33350215 AAACTCATGCCCTGGAGCCCAGAGCAGAGGAGGGCGGCGGGCCCGGGCGGGCTACGGGGCCGGCGCCTCCCCCGCCCCCG
TGGGTCGCGGCGTCTCCGGGGCACTGACCTGCCAGCCGCCTCTCCCGGACGTTCTTCCAC
mm4__chr1 50.33 Mbp_chr1_50333166 GTCCACACTGTCCTTGGGAGGACGCGCGGGGAAGCCGCGCACGATGCGGCAGCGCCCCCTGCCGGGCTCCGGGCGCTCCG
CTCCCAGCCGGCTCGGCGAGTGCTCCGGGAAAG
mm4__chr9 19.10 Mbp_chr9_19102920 CGCCCTTAAGGAAGAGCAGTGTGCGCCGGCGCCGCCAGGCGTCCGAGGGAGGAGGGACTTCCGGCAAAGACTATATCTCC
CGCCATGCAATGCGTCCTTCTCTCCTCCCTTCCG
mm4__chr9 134.82 Mbp_chr9_134818695 AGAGCTCCGGACCTCATTCTGCCCTCCGCCGTCCTGCAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGGAAGACTGGCCC
CATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGAGGGATC
mm4__chr8 96.15 Mbp_chr8_96145306 AGCTCCAAGCACAGCTGCTTCCAGGGCTGGTGGCGCAGGCCCTGCCACACGTCGAAGACTTCCCAGCCGGCCGGCGGCGC
CCCCTGCGGGTCCAGGGTCCGCGCGTCCAGCAGTAGGGGCGAAAGGCAAGGGAAGAGCT
mm4__chr20 46.81 Mbp_chr20_46811116 GCCACCGTCTTCTCCAATGAGAGCGATGACAGGATCTAAGGGGCCCCCTGGCGCTCTAAGGTCCTGGCCAGTGGAGACCC
TGGGGTGCGACAAAGGGGTTAG
mm4__chr19 40.60 Mbp_chr19_40602274 ACGTGCGACAGAAATCCAGATAAGAGGGCTTACGGAAGGGGCGCCCCCTGGGGGCGGAGGGGACAGGAGGTGTATGCACC
AGGCACTCCTAGAGATGGGGA
mm4__chr17 82.96 Mbp_chr17_82960836 GCGCGTGGTCATTTTACCTCTGGGAACTAGGGAAGGACGTTCGGCCTTCACCCTGGCGCTCCCTGGCGCCCTTGGGAAAT
GCCCCTTCGTGGACG
mm4__chr17 145.03 Kbp_chr17_145031 GCTTAACTAGACACCAGGCCTTTTAGAAATAGACCACCTCTTACCTTAGGCCCCCAGAGGGTGCCCATTCTGTGTGGAGA
AAAGAGGAGCCCTTGCC
mm4__chr13 52.45 Mbp_chr13_52450652 TCCAAACGAAGCGGTCCATGGCCGCTGCCACCCAGCCCCGGCCCTCCGAGGCCGCGAGCAGCGCGCCAGGCAGCCTGCGG
CGCCCCCTAGCGTCGGACGGGACGGAAGCGAGTCGGGGCGGGTGAGGGACGGAGGGAG
mm4__chr11 69.01 Mbp_chr11_69013743 GAACTGGCAGAGGTGGTCAGCACCGCCCAAGCGGCACCCCTCCCCCTTCCCACCCCGGACTCGGCCTGGGCCCCCTGGGG
GCTTATGGAACCAGGAATTCCCTTTTCCCCAGCCC
mm4__chr1 966.55 Kbp_chr1_966552 CTGTACGACTCTGGCCATGGGGAACAGCCACTGTGTCCCTCAGGCCCCCAGGAGGCTCCGGGCCTCCTTCTCCAGAAAGC
CCTCGCTGAAGGGAAACAGGT
mm4__chr1 4.16 Mbp_chr1_4161784 CCACCACCACCAAACCTCATGGGGGCGGGGCATGAGCCATGCGGGAGGGGACGCTCTCCATGGGCCCCCAGGAGGCTCCG
TGGTGAGTGACAGACAGTCTGCTGTAAGTAGATGGGTCAGGGCACAC
mm4__chr1 143.72 Mbp_chr1_143717610 CTTGCCCCTAGCCTGCTTTAGAAGCCCCCGGAAACGCCCCGGCCCCGACCCGGACCTGAGCCACCTGGGGGCCTAAGGGA
AGCTGAATGCCCAGTGGGCTCCCGGGATGGCTCTTCCCGTTCTT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.