CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCGCGCCGGCGCCCCCTGG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	(Wild-type sequence)
417.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGC-------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`13 bp deleted → frameshift`
346.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC-----------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`11 bp deleted → frameshift`
344.4	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG---CCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`3 bp deleted → no frameshift`
335.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCT--------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`8 bp deleted → frameshift`
329.4	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGG----------------------------------AAGTGGCCAGGGCGGG`	`34 bp deleted → frameshift`
327.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC--------------------------GCCGGAAGTGGCCAGGGCGGG`	`26 bp deleted → frameshift`
256.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCC-----------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`17 bp deleted → frameshift`
255.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC---------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`9 bp deleted → no frameshift`
247.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC----------------------------GGAAGTGGCCAGGGCGGG`	`28 bp deleted → frameshift`
242.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG----------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`10 bp deleted → frameshift`
242.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC----------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`10 bp deleted → frameshift`
242.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG----------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`10 bp deleted → frameshift`
231.0	`TCCTTTGTCCCCAATCTGGGCGCGC------------------------------------CGGAAGTGGCCAGGGCGGG`	`36 bp deleted → no frameshift`
190.2	`TCCTTTGTCCCCAATCTGGGC-----------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`23 bp deleted → frameshift`
179.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG----------------CGCGCCGGAAGTGGCCAGGGCGGG`	`16 bp deleted → frameshift`
179.6	`TCCTTTGTCCCCAATCTGGGCGCGCG----------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`16 bp deleted → frameshift`
162.8	`TCCTTTGTCCCCAATCTGGGCGCG------------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`18 bp deleted → no frameshift`
161.6	`TCCTTTGTCCCCAATCTGGGCGCGCGC--------------------------------GCCGGAAGTGGCCAGGGCGGG`	`32 bp deleted → frameshift`
154.8	`TCCTTTGTCCCCAATCTGGGCGCGC-------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`19 bp deleted → frameshift`
147.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGG--------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`20 bp deleted → frameshift`
147.2	`TCCTTTGTCCCCAATCTGGGCG--------------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`20 bp deleted → frameshift`
141.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCT---------------CGGCGCGCCGGAAGTGGCCAGGGCGGG`	`15 bp deleted → no frameshift`
140.0	`TCCTTTGTCCCCAATCTGGGCGC---------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`21 bp deleted → no frameshift`
126.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG-----------------------GCGCGCCGGAAGTGGCCAGGGCGGG`	`23 bp deleted → frameshift`
120.4	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG------------------------AAGTGGCCAGGGCGGG`	`24 bp deleted → no frameshift`
120.4	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC------------------------GCGCCGGAAGTGGCCAGGGCGGG`	`24 bp deleted → no frameshift`
120.4	`TCCTTTGTCCCCAATCTGG------------------------CCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`24 bp deleted → no frameshift`
120.0	`TCCTTTGTCCCCAATCTGGGCGC--------------------------------------CGGAAGTGGCCAGGGCGGG`	`38 bp deleted → frameshift`
114.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCC-------------------------GGAAGTGGCCAGGGCGGG`	`25 bp deleted → frameshift`
111.5	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG------------------------------CCAGGGCGGG`	`30 bp deleted → no frameshift`
109.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC--------------------------GGAAGTGGCCAGGGCGGG`	`26 bp deleted → frameshift`
103.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC---------------------------GGAAGTGGCCAGGGCGGG`	`27 bp deleted → no frameshift`
94.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCG-----------------------------CGCCGGAAGTGGCCAGGGCGGG`	`29 bp deleted → frameshift`
94.0	`TCCTTTGTCCCCAATCTGGGCGCGCG-----------------------------GCGCGCCGGAAGTGGCCAGGGCGGG`	`29 bp deleted → frameshift`
90.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC--------------------------------------AGGGCGGG`	`38 bp deleted → frameshift`
89.2	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGC------------------------------CGGAAGTGGCCAGGGCGGG`	`30 bp deleted → no frameshift`
89.2	`TCCTTTGTCCCCAATCTGGGCGCGCGC------------------------------GCGCCGGAAGTGGCCAGGGCGGG`	`30 bp deleted → no frameshift`
89.2	`TCCTTTGTCCCCAATCTGGG------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`30 bp deleted → no frameshift`
84.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG-------------------------------GAAGTGGCCAGGGCGGG`	`31 bp deleted → frameshift`
84.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCG-------------------------------CCGGAAGTGGCCAGGGCGGG`	`31 bp deleted → frameshift`
84.8	`TCCTTTGTCCCCAATCTGGGCGCG-------------------------------GCGCGCCGGAAGTGGCCAGGGCGGG`	`31 bp deleted → frameshift`
84.8	`TCCTTTGTCCCCAATCTGG-------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`31 bp deleted → frameshift`
81.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGC----------------------------------------CAGGGCGGG`	`40 bp deleted → frameshift`
80.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG----------------------------------------------GG`	`46 bp deleted → frameshift`
76.8	`TCCTTTGTCCCCAATCTGGGCG---------------------------------GCGCGCCGGAAGTGGCCAGGGCGGG`	`33 bp deleted → no frameshift`
76.8	`TCCTTTGTCCCC---------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`33 bp deleted → no frameshift`
73.2	`TCCTTTGTCCC----------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`34 bp deleted → frameshift`
70.5	`TCCTTTGTCCCCAATCT-----------------------------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`29 bp deleted → frameshift`
69.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG-----------------------------------GCGGG`	`35 bp deleted → frameshift`
69.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCC-----------------------------------AGGGCGGG`	`35 bp deleted → frameshift`
69.6	`TCCTTTGTCC-----------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`35 bp deleted → frameshift`
66.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCC--------------------------------------------AGGGCGGG`	`44 bp deleted → frameshift`
66.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG------------------------------------CGGG`	`36 bp deleted → no frameshift`
66.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC------------------------------------AGGGCGGG`	`36 bp deleted → no frameshift`
62.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC-------------------------------------AGGGCGGG`	`37 bp deleted → frameshift`
62.8	`TCCTTTGTCCCCAATCTGGGCGCGCG-------------------------------------GAAGTGGCCAGGGCGGG`	`37 bp deleted → frameshift`
62.8	`TCCTTTGTCCCCAATCTGG-------------------------------------CGCGCCGGAAGTGGCCAGGGCGGG`	`37 bp deleted → frameshift`
61.0	`TCCTTTGTCCCCAATCTGGGCG--------------------------------------------------------GG`	`56 bp deleted → frameshift`
61.0	`TCCT------------------------------------------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`42 bp deleted → no frameshift`
56.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG---------------------------------------G`	`39 bp deleted → no frameshift`
56.8	`TCCTTTGTCCCCAATCTGGGCGCG---------------------------------------GAAGTGGCCAGGGCGGG`	`39 bp deleted → no frameshift`
54.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG----------------------------------------`	`40 bp deleted → frameshift`
54.0	`TCCTTTGTCCCCAATCTGGGC----------------------------------------CGGAAGTGGCCAGGGCGGG`	`40 bp deleted → frameshift`
51.6	`TCCTTTGTCCCCAATCTGGGCG-----------------------------------------GAAGTGGCCAGGGCGGG`	`41 bp deleted → frameshift`
51.6	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGG-------------------------------------------------G`	`49 bp deleted → frameshift`
49.5	`TCCTTTGTCCCCAATCT------------------------------------CGGCGCGCCGGAAGTGGCCAGGGCGGG`	`36 bp deleted → no frameshift`
49.2	`TCCTTTGTCCCCAATCTGGGC--------------------------------------------------CAGGGCGGG`	`50 bp deleted → frameshift`
45.0	`TCCTTTGTCCCCAATC--------------------------------------GGCGCGCCGGAAGTGGCCAGGGCGGG`	`38 bp deleted → frameshift`
44.4	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC--------------------------------------------GGG`	`44 bp deleted → frameshift`
44.4	`TCCTTTGTCCCCAATCTGGGCGCGCG----------------------------------------------------GG`	`52 bp deleted → frameshift`
44.4	`TCCTTTGTCCCCAATCTGGG--------------------------------------------AAGTGGCCAGGGCGGG`	`44 bp deleted → frameshift`
42.0	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGG---------------------------------------------GCGGG`	`45 bp deleted → no frameshift`
42.0	`TCCTTTGTCCCCAATCTGG---------------------------------------------AAGTGGCCAGGGCGGG`	`45 bp deleted → no frameshift`
40.2	`TCCTTTGTCCCCAATCTGGGCGCG------------------------------------------------------GG`	`54 bp deleted → no frameshift`
40.0	`TCCTTTGTCCCCAATCTGGGCGCGC----------------------------------------------CAGGGCGGG`	`46 bp deleted → frameshift`
39.0	`TCCTTTGTCCCCAATCTGG---------------------------------------------------CCAGGGCGGG`	`51 bp deleted → no frameshift`
36.6	`TCCTTTGTCCCCAA----------------------------------GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG`	`34 bp deleted → frameshift`
36.4	`TCCTTTGTCCCCAATCTGGGCGC------------------------------------------------CAGGGCGGG`	`48 bp deleted → no frameshift`
32.8	`TCCTTTGTCCCCAATCTGGGCGCGCGCCGG--------------------------------------------------`	`50 bp deleted → frameshift`
32.8	`TCCTTTGTCCCCAATCTGGGCGCGCGC--------------------------------------------------GGG`	`50 bp deleted → frameshift`
32.8	`TCCTTTGTCCCC--------------------------------------------------GGAAGTGGCCAGGGCGGG`	`50 bp deleted → frameshift`
31.5	`TCCTTTGTC---------------------------------------------GGCGCGCCGGAAGTGGCCAGGGCGGG`	`45 bp deleted → no frameshift`
31.2	`TCCTTTGTCCC---------------------------------------------------GGAAGTGGCCAGGGCGGG`	`51 bp deleted → no frameshift`
30.0	`TCCTTTGTCCCCAATCTGGG------------------------------------------------------------`	`60 bp deleted → no frameshift`
29.6	`TCCTTTGTCC----------------------------------------------------GGAAGTGGCCAGGGCGGG`	`52 bp deleted → frameshift`
25.8	`TCCT-------------------------------------------------CGGCGCGCCGGAAGTGGCCAGGGCGGG`	`49 bp deleted → frameshift`
25.6	`TCCTTTGTCCCCAATCTGGG-------------------------------------------------------GCGGG`	`55 bp deleted → frameshift`
25.0	`TCCTTTGTCCCCA------------------------------------------------------------GGGCGGG`	`60 bp deleted → no frameshift`
23.2	`TCCTTTGTCCCCAATCTGG---------------------------------------------------------CGGG`	`57 bp deleted → no frameshift`
22.2	`TC----------------------------------------------------GGCGCGCCGGAAGTGGCCAGGGCGGG`	`52 bp deleted → frameshift`
20.8	`TCCTTTGTCCCCAATCTGGG-----------------------------------------------------------G`	`59 bp deleted → frameshift`
20.8	`TCC-----------------------------------------------------------GGAAGTGGCCAGGGCGGG`	`59 bp deleted → frameshift`
18.8	`TCCTTTGTCCCCAATCTGG-------------------------------------------------------------`	`61 bp deleted → frameshift`
18.8	`TCCTTTGTCCC-------------------------------------------------------------AGGGCGGG`	`61 bp deleted → frameshift`
18.0	`TCCTTTGTCC--------------------------------------------------------------AGGGCGGG`	`62 bp deleted → frameshift`
15.0	`TCCTTTGT------------------------------------------------------------GGCCAGGGCGGG`	`60 bp deleted → no frameshift`
14.8	`TCCTTTGTCCCCAA----------------------------------------------------GTGGCCAGGGCGGG`	`52 bp deleted → frameshift`
13.5	`TCCTTTG--------------------------------------------------------------GCCAGGGCGGG`	`62 bp deleted → frameshift`
12.8	`TCC---------------------------------------------------------------------AGGGCGGG`	`69 bp deleted → no frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License