CRISPOR

Micro-homology scoring of potential deletions

Guide: TGGAGGGGAAGACGGCCCGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	(Wild-type sequence)
427.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-----------------CGAAGCCGGCCTTGCACATGCCGG`	`17 bp deleted → frameshift`
382.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`9 bp deleted → no frameshift`
362.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG--GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`2 bp deleted → frameshift`
359.2	`GTGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`16 bp deleted → frameshift`
344.4	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG---GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`3 bp deleted → no frameshift`
327.6	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG----CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`4 bp deleted → frameshift`
283.2	`GTGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`15 bp deleted → no frameshift`
282.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`7 bp deleted → frameshift`
268.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG--------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`8 bp deleted → frameshift`
256.2	`GTGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`17 bp deleted → frameshift`
242.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG----------TCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`10 bp deleted → frameshift`
229.6	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG-------------------------CCTTGCACATGCCGG`	`25 bp deleted → frameshift`
223.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------------------TTGCACATGCCGG`	`30 bp deleted → no frameshift`
208.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------CCCGCGAAGCCGGCCTTGCACATGCCGG`	`13 bp deleted → frameshift`
198.8	`GTGCCTGGGGCGCCCCACGATGGAGGGG--------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`14 bp deleted → frameshift`
188.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`15 bp deleted → no frameshift`
183.0	`GTGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`34 bp deleted → frameshift`
179.6	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC----------------CGCGAAGCCGGCCTTGCACATGCCGG`	`16 bp deleted → frameshift`
163.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC--------------------------GGCCTTGCACATGCCGG`	`26 bp deleted → frameshift`
162.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------GCGAAGCCGGCCTTGCACATGCCGG`	`18 bp deleted → no frameshift`
162.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------CCCGCGAAGCCGGCCTTGCACATGCCGG`	`18 bp deleted → no frameshift`
162.8	`GTGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`18 bp deleted → no frameshift`
154.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------------AAGCCGGCCTTGCACATGCCGG`	`19 bp deleted → frameshift`
154.8	`GTGCCTGGGGCGCCCCACGATGG-------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`19 bp deleted → frameshift`
147.2	`GTGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`20 bp deleted → frameshift`
140.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------------------GAAGCCGGCCTTGCACATGCCGG`	`21 bp deleted → no frameshift`
140.0	`GTGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`21 bp deleted → no frameshift`
135.0	`GTGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCACATGCCGG`	`40 bp deleted → frameshift`
133.8	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCACATGCCGG`	`30 bp deleted → no frameshift`
133.2	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG----------------------CGAAGCCGGCCTTGCACATGCCGG`	`22 bp deleted → frameshift`
120.4	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------------AAGCCGGCCTTGCACATGCCGG`	`24 bp deleted → no frameshift`
115.2	`GTGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`33 bp deleted → no frameshift`
108.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG----------------------------------------`	`40 bp deleted → frameshift`
104.4	`GTGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`35 bp deleted → frameshift`
94.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-----------------------------TTGCACATGCCGG`	`29 bp deleted → frameshift`
89.2	`GTGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`30 bp deleted → no frameshift`
85.2	`GTGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCACATGCCGG`	`39 bp deleted → no frameshift`
80.8	`GTGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`32 bp deleted → frameshift`
80.8	`GTGCCTGGGG--------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`32 bp deleted → frameshift`
77.7	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCACATGCCGG`	`27 bp deleted → no frameshift`
77.4	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC-----------------------------------------GG`	`41 bp deleted → frameshift`
76.8	`GTGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCACATGCCGG`	`33 bp deleted → no frameshift`
71.0	`GTGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCACATGCCGG`	`39 bp deleted → no frameshift`
70.5	`GTGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`29 bp deleted → frameshift`
69.6	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------ACATGCCGG`	`35 bp deleted → frameshift`
66.6	`GTGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCACATGCCGG`	`44 bp deleted → frameshift`
66.0	`GTGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`36 bp deleted → no frameshift`
63.0	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG---------------------------------------------`	`45 bp deleted → no frameshift`
62.8	`GTGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCACATGCCGG`	`37 bp deleted → frameshift`
62.8	`GTGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCACATGCCGG`	`37 bp deleted → frameshift`
62.8	`GTGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`37 bp deleted → frameshift`
60.0	`GTGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCACATGCCGG`	`38 bp deleted → frameshift`
60.0	`GTGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCACATGCCGG`	`38 bp deleted → frameshift`
60.0	`GTGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCACATGCCGG`	`46 bp deleted → frameshift`
56.8	`GTGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCACATGCCGG`	`39 bp deleted → no frameshift`
54.6	`GTGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`26 bp deleted → frameshift`
52.2	`GTGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCACATGCCGG`	`35 bp deleted → frameshift`
51.6	`GTGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCACATGCCGG`	`41 bp deleted → frameshift`
51.6	`GTGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`41 bp deleted → frameshift`
51.6	`GTGCCTGGGGCGCC-------------------------------------------------GGCCTTGCACATGCCGG`	`49 bp deleted → frameshift`
51.6	`GTGCC-------------------------------------------------CGCGAAGCCGGCCTTGCACATGCCGG`	`49 bp deleted → frameshift`
49.5	`GTGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCACATGCCGG`	`36 bp deleted → no frameshift`
48.8	`GTGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCACATGCCGG`	`42 bp deleted → no frameshift`
48.8	`GTGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCACATGCCGG`	`42 bp deleted → no frameshift`
42.6	`GTGCCTGGGGCGCC-----------------------------------------------------TTGCACATGCCGG`	`53 bp deleted → frameshift`
42.0	`GTGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCACATGCCGG`	`45 bp deleted → no frameshift`
40.5	`GTGCCTGGGGCGCCCCACGATGGAGGGGAAGAC----------------------------------------ATGCCGG`	`40 bp deleted → frameshift`
38.4	`GTGCCTGGGGC-------------------------------------------------------CTTGCACATGCCGG`	`55 bp deleted → frameshift`
38.0	`GTGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCACATGCCGG`	`47 bp deleted → frameshift`
36.4	`GTGCCTGGGGCGCCC------------------------------------------------GGCCTTGCACATGCCGG`	`48 bp deleted → no frameshift`
33.0	`GTGCC----------------------------------------------------------GGCCTTGCACATGCCGG`	`58 bp deleted → frameshift`
32.8	`GTGCC--------------------------------------------------GCGAAGCCGGCCTTGCACATGCCGG`	`50 bp deleted → frameshift`
32.0	`GTGCCTGGGGCGCCCCAC-------------------------------------------------------ATGCCGG`	`55 bp deleted → frameshift`
31.5	`GTGCCT--------------------------------------------------------------TGCACATGCCGG`	`62 bp deleted → frameshift`
31.2	`GTGCCTGGGGCGCCCC---------------------------------------------------TTGCACATGCCGG`	`51 bp deleted → no frameshift`
31.2	`GTGCCTGGGGC---------------------------------------------------CGGCCTTGCACATGCCGG`	`51 bp deleted → no frameshift`
29.6	`GTGCCTGGGGCGCCCCACGATGGAGGGG----------------------------------------------------`	`52 bp deleted → frameshift`
29.6	`GTGCCTGGGGCGCCC----------------------------------------------------TTGCACATGCCGG`	`52 bp deleted → frameshift`
29.6	`GTGCCTGGGGCG----------------------------------------------------GCCTTGCACATGCCGG`	`52 bp deleted → frameshift`
28.4	`GTGCCTGGGGCGCCCCACGATGGAGGG-----------------------------------------------------`	`53 bp deleted → frameshift`
28.4	`GTGC-----------------------------------------------------GAAGCCGGCCTTGCACATGCCGG`	`53 bp deleted → frameshift`
27.3	`GTGCCTGGGGCGCCCCACGATG------------------------------------------------CACATGCCGG`	`48 bp deleted → no frameshift`
27.3	`GT------------------------------------------------CGCCCGCGAAGCCGGCCTTGCACATGCCGG`	`48 bp deleted → no frameshift`
26.8	`GTGCCTGGGGCGCCCCACGATGGAGG------------------------------------------------------`	`54 bp deleted → no frameshift`
26.8	`GTGCCTGGGGCGCCCCACGATG------------------------------------------------------CCGG`	`54 bp deleted → no frameshift`
24.6	`GTGCCTGGGGCGCC----------------------------------------------------------------GG`	`64 bp deleted → frameshift`
24.4	`GTGCCTGGG--------------------------------------------------------CCTTGCACATGCCGG`	`56 bp deleted → frameshift`
23.2	`GTGCCTGGGGCGCCCCACGATGG---------------------------------------------------------`	`57 bp deleted → no frameshift`
23.2	`GTGCCTGG---------------------------------------------------------CCTTGCACATGCCGG`	`57 bp deleted → no frameshift`
22.0	`GTGCCTGGGGCGC----------------------------------------------------------ACATGCCGG`	`58 bp deleted → frameshift`
20.0	`GTGCCTGGGGCGCCCCACG------------------------------------------------------------G`	`60 bp deleted → no frameshift`
20.0	`GTGCCTGGGGC------------------------------------------------------------ACATGCCGG`	`60 bp deleted → no frameshift`
18.2	`GTGCC-------------------------------------------------------------------------GG`	`73 bp deleted → frameshift`
18.0	`GTGCCTGGGGCGCCCC--------------------------------------------------------------GG`	`62 bp deleted → frameshift`
17.5	`GTGC-------------------------------------------------------------------ACATGCCGG`	`67 bp deleted → frameshift`
17.4	`GTGCCTGGGGCGCCCCA---------------------------------------------------------TGCCGG`	`57 bp deleted → no frameshift`
17.2	`GTGCCTGGGGCGCCC---------------------------------------------------------------GG`	`63 bp deleted → no frameshift`
14.8	`GTGCCTGGGGC------------------------------------------------------------------CGG`	`66 bp deleted → no frameshift`
14.0	`GTGCCTGGGGCG-------------------------------------------------------------------G`	`67 bp deleted → frameshift`
12.9	`GTGCCTG---------------------------------------------------------------CACATGCCGG`	`63 bp deleted → no frameshift`
12.0	`GTGCCTGGGG----------------------------------------------------------------------`	`70 bp deleted → frameshift`
11.6	`GTGCCTGGG-----------------------------------------------------------------------`	`71 bp deleted → frameshift`
10.8	`GTGCCTGG------------------------------------------------------------------------`	`72 bp deleted → no frameshift`
9.6	`GTGCCTG---------------------------------------------------------------------CCGG`	`69 bp deleted → no frameshift`

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