← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529706_F | TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC | 60.1 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529706_R | GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT | 60.6 | 1.00 |
| mm4__chr15 89.38 Mbp_chr15_89379496_F | TCGTCGGCAGCGTCCCGCGTTTTCTTGGAACCAC | 60.3 | 0.53 |
| mm4__chr15 89.38 Mbp_chr15_89379496_R | GTCTCGTGGGCTCGGGTCCTCAGTCCCAACCACAG | 59.9 | 0.53 |
| mm4__chr7 157.05 Mbp_chr7_157049256_F | TCGTCGGCAGCGTCCCCACACTCAGGAACAGCC | 60.3 | 0.52 |
| mm4__chr7 157.05 Mbp_chr7_157049256_R | GTCTCGTGGGCTCGGCATGGCCTTGGTGAGGGATG | 60.7 | 0.52 |
| mm4__chr22 49.73 Mbp_chr22_49728059_F | TCGTCGGCAGCGTCGCTGTCTGTGAGCTCTGACC | 60.3 | 0.40 |
| mm4__chr22 49.73 Mbp_chr22_49728059_R | GTCTCGTGGGCTCGGACTGCGTCTCCAGTTAACGG | 60.0 | 0.40 |
| mm4__chr16 18.50 Mbp_chr16_18499299_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 18.50 Mbp_chr16_18499299_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 18.42 Mbp_chr16_18416700_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 18.42 Mbp_chr16_18416700_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 16.30 Mbp_chr16_16295412_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 16.30 Mbp_chr16_16295412_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 14.90 Mbp_chr16_14896758_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr16 14.90 Mbp_chr16_14896758_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr7 39.95 Mbp_chr7_39950088_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr7 39.95 Mbp_chr7_39950088_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr10 48.51 Mbp_chr10_48509605_F | TCGTCGGCAGCGTCCTCCAGCCAGGACAATGGG | 60.0 | 0.33 |
| mm4__chr10 48.51 Mbp_chr10_48509605_R | GTCTCGTGGGCTCGGTGATAGTGGGGAGGATGGGG | 60.1 | 0.33 |
| mm4__chr17 82.02 Mbp_chr17_82023280_F | TCGTCGGCAGCGTCCTTTGTTCTCATTGGCCCGC | 60.1 | 0.32 |
| mm4__chr17 82.02 Mbp_chr17_82023280_R | GTCTCGTGGGCTCGGGGGGCCTCAGTATCCAGGA | 60.0 | 0.32 |
| mm3__chr7 44.89 Mbp_chr7_44885526_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm3__chr7 44.89 Mbp_chr7_44885526_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm3__chr20 56.47 Mbp_chr20_56468607_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3__chr20 56.47 Mbp_chr20_56468607_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr8 143.82 Mbp_chr8_143818331_F | TCGTCGGCAGCGTCAAGCCCTAGACACAGGGACA | 60.1 | 0.25 |
| mm4__chr8 143.82 Mbp_chr8_143818331_R | GTCTCGTGGGCTCGGGACTCCGTCACCATGAAGCA | 60.0 | 0.25 |
| mm4__chr1 103.96 Mbp_chr1_103961097_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr1 103.96 Mbp_chr1_103961097_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr15 51.43 Mbp_chr15_51429404_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr15 51.43 Mbp_chr15_51429404_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr2 177.39 Mbp_chr2_177393000_F | TCGTCGGCAGCGTCTACCAATGAGTGCAAAGCGC | 59.4 | 0.22 |
| mm4__chr2 177.39 Mbp_chr2_177393000_R | GTCTCGTGGGCTCGGCCTCGACGCTAACTCCTTCC | 59.8 | 0.22 |
| mm4__chr15 42.14 Mbp_chr15_42142752_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4__chr15 42.14 Mbp_chr15_42142752_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4__chr19 13.21 Mbp_chr19_13207593_F | TCGTCGGCAGCGTCCCATCGGCCTCGTCGTAGT | 61.4 | 0.19 |
| mm4__chr19 13.21 Mbp_chr19_13207593_R | GTCTCGTGGGCTCGGCCCAGGGCCTGTCGACAC | 62.3 | 0.19 |
| mm4__chr11 120.86 Mbp_chr11_120862615_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr11 120.86 Mbp_chr11_120862615_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr5 128.08 Mbp_chr5_128083983_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr5 128.08 Mbp_chr5_128083983_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr19 22.89 Mbp_chr19_22893821_F | TCGTCGGCAGCGTCCTTTCCGCGGGCAGAGTG | 61.1 | 0.16 |
| mm4__chr19 22.89 Mbp_chr19_22893821_R | GTCTCGTGGGCTCGGGTAACCAGAGAGGCAGGACG | 59.8 | 0.16 |
| mm4__chrX 108.73 Mbp_chrX_108734827_F | TCGTCGGCAGCGTCCTACTGGAGCCTTGGCCATT | 59.7 | 0.16 |
| mm4__chrX 108.73 Mbp_chrX_108734827_R | GTCTCGTGGGCTCGGTTTGGGGAGGAAGGCAATCC | 59.9 | 0.16 |
| mm4__chr2 37.67 Mbp_chr2_37671339_F | TCGTCGGCAGCGTCTAGATGGGAAAGCGATGCCC | 59.8 | 0.14 |
| mm4__chr2 37.67 Mbp_chr2_37671339_R | GTCTCGTGGGCTCGGCTGTCTGTGAGTCTGTCCCG | 59.7 | 0.14 |
| mm4__chr9 127.79 Mbp_chr9_127786129_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr9 127.79 Mbp_chr9_127786129_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr11 1.03 Mbp_chr11_1033178_F | TCGTCGGCAGCGTCCCGTTTCCCTGCACACACT | 60.5 | 0.13 |
| mm4__chr11 1.03 Mbp_chr11_1033178_R | GTCTCGTGGGCTCGGGAGAGCAGGGAAGGAAGCTG | 60.1 | 0.13 |
| mm3__chr2 235.62 Mbp_chr2_235619924_F | TCGTCGGCAGCGTCATGCTGGGCTTGGTTTTCCT | 60.1 | 0.12 |
| mm3__chr2 235.62 Mbp_chr2_235619924_R | GTCTCGTGGGCTCGGGCCATGCCACCAAGAACAAC | 60.3 | 0.12 |
| mm4__chr4 7.77 Mbp_chr4_7774518_F | TCGTCGGCAGCGTCAATCTCTGTTGTCAGCGCCA | 59.9 | 0.12 |
| mm4__chr4 7.77 Mbp_chr4_7774518_R | GTCTCGTGGGCTCGGCAGGTGCCTGTGTGGTCAAA | 60.7 | 0.12 |
| mm4__chr9 129.84 Mbp_chr9_129835267_F | TCGTCGGCAGCGTCGAGTTGTACAGCCGCCGC | 61.4 | 0.11 |
| mm4__chr9 129.84 Mbp_chr9_129835267_R | GTCTCGTGGGCTCGGCTGACGCCAGGAGAGGGG | 61.4 | 0.11 |
| mm4__chr4 188.56 Mbp_chr4_188555324_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr4 188.56 Mbp_chr4_188555324_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr8 10.05 Mbp_chr8_10054799_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr8 10.05 Mbp_chr8_10054799_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr2 109.13 Mbp_chr2_109130122_F | TCGTCGGCAGCGTCCGGCGGCAAAGGTGAGTATC | 61.4 | 0.09 |
| mm4__chr2 109.13 Mbp_chr2_109130122_R | GTCTCGTGGGCTCGGTCTCCTCCGCACCTCATCA | 60.0 | 0.09 |
| mm3__chr1 7.77 Mbp_chr1_7771211_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3__chr1 7.77 Mbp_chr1_7771211_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr12 31.59 Mbp_chr12_31590997_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr12 31.59 Mbp_chr12_31590997_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr15 81.00 Mbp_chr15_81002195_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr15 81.00 Mbp_chr15_81002195_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 124.74 Mbp_chr10_124744495_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 124.74 Mbp_chr10_124744495_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr12 51.81 Mbp_chr12_51814460_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr12 51.81 Mbp_chr12_51814460_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr15 42.74 Mbp_chr15_42737138_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr15 42.74 Mbp_chr15_42737138_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr5 93.59 Mbp_chr5_93587677_F | TCGTCGGCAGCGTCTCTTGACCTTGCCAGGGTTG | 60.1 | 0.05 |
| mm4__chr5 93.59 Mbp_chr5_93587677_R | GTCTCGTGGGCTCGGTAGGAGATCTGGGGTGAGGC | 60.1 | 0.05 |
| mm4__chr22 46.00 Mbp_chr22_46001598_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr22 46.00 Mbp_chr22_46001598_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr9 122.23 Mbp_chr9_122226248_F | TCGTCGGCAGCGTCCTGGAGCTCTGGGTTCGC | 60.1 | 0.04 |
| mm4__chr9 122.23 Mbp_chr9_122226248_R | GTCTCGTGGGCTCGGCATTCGGAGGCCACTTTTGC | 60.1 | 0.04 |
| mm4__chr12 45.05 Mbp_chr12_45050711_F | TCGTCGGCAGCGTCCAGCTTTAGGGGAACTGGGC | 60.6 | 0.04 |
| mm4__chr12 45.05 Mbp_chr12_45050711_R | GTCTCGTGGGCTCGGGATCGAGAATTTGCTGCGGG | 59.6 | 0.04 |
| mm4__chr3 149.75 Mbp_chr3_149752484_F | TCGTCGGCAGCGTCTGTCCTACGATTTCACCCGG | 59.4 | 0.04 |
| mm4__chr3 149.75 Mbp_chr3_149752484_R | GTCTCGTGGGCTCGGCGTCCGAGTGTAACGCGTTA | 60.4 | 0.04 |
| mm4__chr14 49.63 Mbp_chr14_49634038_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr14 49.63 Mbp_chr14_49634038_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 58.90 Mbp_chr1_58903690_F | TCGTCGGCAGCGTCGCGCTGGGAGTCTCAGTG | 60.1 | 0.03 |
| mm4__chr1 58.90 Mbp_chr1_58903690_R | GTCTCGTGGGCTCGGTCCCTCCACCCGCTCCTC | 62.7 | 0.03 |
| mm4__chr12 109.31 Mbp_chr12_109309149_F | TCGTCGGCAGCGTCAAAGCCGACCCTGCAAGG | 60.2 | 0.03 |
| mm4__chr12 109.31 Mbp_chr12_109309149_R | GTCTCGTGGGCTCGGGCGGCCAAATTGCGGAAC | 60.8 | 0.03 |
| mm4__chr1 95.12 Mbp_chr1_95117782_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 95.12 Mbp_chr1_95117782_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr2 5.71 Mbp_chr2_5706781_F | TCGTCGGCAGCGTCCTGGTGTCCGTGAGTCTGAC | 60.0 | 0.02 |
| mm4__chr2 5.71 Mbp_chr2_5706781_R | GTCTCGTGGGCTCGGCGTTCAGTGTCCAGCGAGT | 60.0 | 0.02 |
| mm4__chr1 35.19 Mbp_chr1_35192554_F | TCGTCGGCAGCGTCCTGAGGAGGTGTGGTAGGGA | 59.9 | 0.01 |
| mm4__chr1 35.19 Mbp_chr1_35192554_R | GTCTCGTGGGCTCGGCACTCCACCAACCTCGGG | 59.6 | 0.01 |
| mm4__chr13 97.11 Mbp_chr13_97110021_F | TCGTCGGCAGCGTCCTGCTCTGGATGCAGTCTGG | 60.4 | 0.01 |
| mm4__chr13 97.11 Mbp_chr13_97110021_R | GTCTCGTGGGCTCGGCTCTGCTGGACGGAACCC | 59.7 | 0.01 |
| mm3__chr18 62.19 Mbp_chr18_62187625_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr18 62.19 Mbp_chr18_62187625_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr16 67.43 Mbp_chr16_67432275_F | TCGTCGGCAGCGTCCTCCTTTGTCTGGAGCAGGG | 60.0 | 0.01 |
| mm4__chr16 67.43 Mbp_chr16_67432275_R | GTCTCGTGGGCTCGGCTCCCACGATGCAATGGAGT | 60.1 | 0.01 |
| mm2__chr4 4.86 Mbp_chr4_4863052_F | TCGTCGGCAGCGTCCTTCGGCCTCTCCTTCCCTC | 61.6 | 0.01 |
| mm2__chr4 4.86 Mbp_chr4_4863052_R | GTCTCGTGGGCTCGGCACATGGGCCGTGTAGAGTC | 60.4 | 0.01 |
| mm4__chr2 218.96 Mbp_chr2_218959936_F | TCGTCGGCAGCGTCCCCGAGGAGAAGAAGAAGGC | 59.8 | 0.00 |
| mm4__chr2 218.96 Mbp_chr2_218959936_R | GTCTCGTGGGCTCGGCGAGATGAGCACGGACGG | 60.2 | 0.00 |
| mm4__chr17 45.02 Mbp_chr17_45019359_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 45.02 Mbp_chr17_45019359_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 822.53 Kbp_chr19_822532_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 822.53 Kbp_chr19_822532_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr20 58.52 Mbp_chr20_58523203_F | TCGTCGGCAGCGTCAACTTAGCCGTCTCGCCTTG | 60.3 | 0.00 |
| mm4__chr20 58.52 Mbp_chr20_58523203_R | GTCTCGTGGGCTCGGCCCCACCTCTGAGACAGACA | 60.5 | 0.00 |
| mm4__chr16 3.11 Mbp_chr16_3106531_F | TCGTCGGCAGCGTCTGCTCCTCACGGCGATTG | 60.1 | 0.00 |
| mm4__chr16 3.11 Mbp_chr16_3106531_R | GTCTCGTGGGCTCGGCACCCTAGCTTGGCGGAC | 60.1 | 0.00 |
| mm4__chr11 75.56 Mbp_chr11_75562009_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 75.56 Mbp_chr11_75562009_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529706 |
TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG |
| mm4__chr15 89.38 Mbp_chr15_89379496 |
CCGCGTTTTCTTGGAACCACGAAGCCCAAAATGGCGAGCGGGCCGTGGCCGCCCTCCTGGGGCTGAGTGTGGTCCGGAGC GCTGAGAGTGGCCAGGGAGCCGTGGGCCTCCATCGTCCCTGTGGTTGGGACTGAGGAC |
| mm4__chr7 157.05 Mbp_chr7_157049256 |
CCCACACTCAGGAACAGCCTGCCGCTTTCGTGACCCGTGGCCACTCCCTGTGCTCCGTGTGGCTCTCCCCACATCCCTCA CCAAGGCCATG |
| mm4__chr22 49.73 Mbp_chr22_49728059 |
GCTGTCTGTGAGCTCTGACCCGGCAATTCGGAAGTGGCCATGGGGACTGCATGTTTGGAATAGGGGTCTCCATCCCTCCG TTAACTGGAGACGCAGT |
| mm4__chr10 48.51 Mbp_chr10_48509605 |
CTCCAGCCAGGACAATGGGGCCTCACAGCAGAGGCAGGGCAGGATGCTGGGTGACCCTGGCCTCTTCCGGGGCTCCACAG CCTCATTTCCCCCAGACTCGCTGGGCCCTCCCCGCACAGCCCCATCCTCCCCACTATCA |
| mm4__chr17 82.02 Mbp_chr17_82023280 |
CTTTGTTCTCATTGGCCCGCACCAAGCGCTGCCTGCGCAGAGCCTGCTAGGAGTTGTAGTTCCCGCGGCCTCTCCCGGCG CGCCTTGTTCTTCCTGGATACTGAGGCCCC |
| mm4__chr8 143.82 Mbp_chr8_143818331 |
AAGCCCTAGACACAGGGACACACCTGTCAGGCTGCGCGAGCCCAGGGGTGGGGGCGAGCCCGAAGTGGCCGGGGCGGACC AAGCCTGCTGACCTTGTGCTTCATGGTGACGGAGTC |
| mm4__chr2 177.39 Mbp_chr2_177393000 |
TACCAATGAGTGCAAAGCGCGGAGAGCCGCGTCGGCGGCCACGGCAGCGCCCACGGCCACTCCCGCCGCGCAGGAGTCGG GCACCATCCCAAAGAAGCGGTGAGTAGCGGTATGTGGAAGGAGTTAGCGTCGAGG |
| mm4__chr19 13.21 Mbp_chr19_13207593 |
CCATCGGCCTCGTCGTAGTCGGAGCCCCGGTAGTAGCCATGGTGCCGGGGACCCGGGGGCCCCTCGGACACGTGCGGGCC AGATGCCGGCCACCGGGCCCCGCCGTGTCGACAGGCCCTGGG |
| mm4__chr19 22.89 Mbp_chr19_22893821 |
CTTTCCGCGGGCAGAGTGGCAGGTGCCGCGCCGGAGGTGGTCATGGTGGAGTGGGCGTCCCTGCTCGGCGTCCTGCCTCT CTGGTTAC |
| mm4__chrX 108.73 Mbp_chrX_108734827 |
CTACTGGAGCCTTGGCCATTTGAGCCCTGGCCACCTCCTGAGCCCCGGCCATTTCCTGAGCCCCAATTGTTCATAGGCAT GTAGTCACCTCCGCTTCCTTCCTGATCTTCTTTGCCCTGGGGATTGCCTTCCTCCCCAAA |
| mm4__chr2 37.67 Mbp_chr2_37671339 |
TAGATGGGAAAGCGATGCCCTGGGTATCCCGACTCCCTGCTGGTTTCCCAGCCTAACACCAGCGCACGCCGGAAGCGGCC ACGGCCGCGGGCGGGACAGACTCACAGACAG |
| mm4__chr11 1.03 Mbp_chr11_1033178 |
CCGTTTCCCTGCACACACTCGGCGTGCGAGAAGTGTCCATGGCCCGTGGGGCTCGAGGCCTCAACAGCAAGCCAAGCGCT TGGCCTCCCATGCTGTCCTTCACGAGCCCCAGCTTCCTTCCCTGCTCTC |
| mm3__chr2 235.62 Mbp_chr2_235619924 | ATGCTGGGCTTGGTTTTCCTGAGCCTTGGCCACTGCTGGCGCCCCGATCCCGGGCCAGTTGTTCTTGGTGGCATGGC |
| mm4__chr4 7.77 Mbp_chr4_7774518 |
AATCTCTGTTGTCAGCGCCAGAAGCCGGAACAGGTCCCCCAGCACCTCCCCTGCCTCGGTGAGCAGCGTGTGGGTCTGGC CCTGGCCTCTGCCTGCACGCCGCATGTTTGACCACACAGGCACCTG |
| mm4__chr9 129.84 Mbp_chr9_129835267 |
GAGTTGTACAGCCGCCGCGCCTCTGCGCAGCGGCCCAGGCTGCTTCCGGCGCGCGGCAGAGCGGTCAGAGCGCCTGCCCC GTCTGGCCCCGCCCCCGGCACGCACAGTCCGCGTGGCCACGCCCCCTCTCCTGGCGTCAG |
| mm4__chr2 109.13 Mbp_chr2_109130122 |
CGGCGGCAAAGGTGAGTATCTGTCTCGGCGGAAGTGGCCACGGCACGTGGGAGTGTGTGGGTGGGTGCTTGGGCGTGGGG GGCAGTGATGAGGTGCGGAGGAGA |
| mm4__chr5 93.59 Mbp_chr5_93587677 |
TCTTGACCTTGCCAGGGTTGGGGCCCTGGAGTCAGGCCTAGCTCCGGCGGGGCTGACGTGGCCAGGGGCTCAGGCAGTGG CTGGACACACTGAGCTGTGGAGCTGGAGGGCCTCACCCCAGATCTCCTA |
| mm4__chr9 122.23 Mbp_chr9_122226248 |
CTGGAGCTCTGGGTTCGCGCCGCTGAGCGCCGGCAGGTTGGACCAGCGCTGCGCGCCGGAAGTGCACTCGGGACGCCGGG GTTCTGGAGGAGAGACCACACGCCGCAAAAGTGGCCTCCGAATG |
| mm4__chr12 45.05 Mbp_chr12_45050711 |
CAGCTTTAGGGGAACTGGGCTAGCAGGCAGGGGCCGGAGCTGCGCGCCCGCGGTGGCCAGGGCGGTCGCCGGGTCGTGGG GCGCGGGCGGCTGCAGCCTGGGCGTTGGGGCGCCCCCGACCCGCAGCAAATTCTCGATC |
| mm4__chr3 149.75 Mbp_chr3_149752484 |
TGTCCTACGATTTCACCCGGCAGCGCCGACCCCGCCTTCGCCACTTCCGGCGCCCGTGTCCCGGCGCCTAGAGGCTCCGG CGTCGCAGCGTCCGGAGCTTTGGGTTCCGGCCTACGAACTAACGCGTTACACTCGGACG |
| mm4__chr1 58.90 Mbp_chr1_58903690 |
GCGCTGGGAGTCTCAGTGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGTCCCGCCCGCCGCCCGGCTTCCCGCC CATGGGCCTCGGCAGTGCCCGCGGGAGGAGCGGGTGGAGGGA |
| mm4__chr12 109.31 Mbp_chr12_109309149 |
AAAGCCGACCCTGCAAGGCGGTACCTGGAGCCGATCCTCGCGGGGCCGCCGCTGCCGGCGCTCCGGGGGTGGGCAGGGGT TCCGGAGGGGGGCTCCCTCGCGCTCGCCCCTCGCGTTCCGCAATTTGGCCGC |
| mm4__chr2 5.71 Mbp_chr2_5706781 |
CTGGTGTCCGTGAGTCTGACGGCGAGCTGGGCAGGTGTGACCTGGAAGGGCGGGCAGGTGGGGCACGGCGCCCCGGAGGG AGCCAGGGGCCACTCGCTGGACACTGAACG |
| mm4__chr1 35.19 Mbp_chr1_35192554 |
CTGAGGAGGTGTGGTAGGGACCCCGCTGCTTGGCGGGGTGGGTGGCGGCGCCCCGGGAGGGGCCGAGGTGACTGCAGGCG GCGGGGTCGGAGTCGGGCCTGGCCCGGACCCTGGCGGGGCCCCCGAGGTTGGTGGAGTG |
| mm4__chr13 97.11 Mbp_chr13_97110021 |
CTGCTCTGGATGCAGTCTGGTGTTACATAACCGGCTGGCTGAGGACGCAGGTCCCCTGGGCGCTGCCGGCGCGCTGTGAC CAGACACGCGGGCGCGGGTGGGGGGTTCCGTCCAGCAGAG |
| mm4__chr16 67.43 Mbp_chr16_67432275 |
CTCCTTTGTCTGGAGCAGGGAATAAATCTGTCACCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGAGGCCCGCCGG AAGTGGGGAGGGGGAGAAAGGCCAGGGGGCACTGACTCCATTGCATCGTGGGAG |
| mm2__chr4 4.86 Mbp_chr4_4863052 |
CTTCGGCCTCTCCTTCCCTCTCGGCGGCCCCGCAGCTGTAGCGGCCGCGGCGGGTGCCTCGCTCTACGGTGCCTCTGGCC CCTTCCAGCGCGCCGCGCTGCCTGTGGCGCCCGTGGGACTCTACACGGCCCATGTG |
| mm4__chr2 218.96 Mbp_chr2_218959936 |
CCCGAGGAGAAGAAGAAGGCGCCGCCCGCGGGGGACGAGGCGCTGGGGGGCTACGGGGCGCCGCCAGTGGGCAAGGGCGG CAAAGGCGAGAGCCGACTCAAGCGGCCGTCCGTGCTCATCTCG |
| mm4__chr20 58.52 Mbp_chr20_58523203 |
AACTTAGCCGTCTCGCCTTGCGGCCTGGAACCCCTGGCAATTTCCGGAGCACCGGCAAGAGACTTTTGCACAGTTTCTGC TTGGAGTCTACCGACTGTCTGTCTCAGAGGTGGGG |
| mm4__chr16 3.11 Mbp_chr16_3106531 |
TGCTCCTCACGGCGATTGCCCCCGCGCCCCGGGCTGGACAGGTGGGGCGCCGCGAAAAGGCCCGGCAAGCCGGAAGTTGC AATGGGGCAGCCGGACGCAAAACGCGGGGACACCGGCCGGGGTCCGCCAAGCTAGGGTG |