CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCCGGCGCGCGCCCAGATT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	(Wild-type sequence)
240.8	`GTCCTTAGGCCGCCAGGGGG------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`24 bp deleted → no frameshift`
229.6	`GTCCTTAGGCCGCCAGGGG-------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`25 bp deleted → frameshift`
211.5	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA-------CAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`7 bp deleted → frameshift`
202.0	`GTCCTTAGGCCGCCAGGGGGCGCCGG--------------------------------GCCGGCCGCGTTATTACCATAA`	`32 bp deleted → frameshift`
199.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC----------------------GGGCCGGCCGCGTTATTACCATAA`	`22 bp deleted → frameshift`
190.2	`GTCCTTAGGCCGCCAGGGGG-----------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`23 bp deleted → frameshift`
188.4	`GTCCTTAGGCCGCCAGGGGGCGCCGGC-------------------------------------CGCGTTATTACCATAA`	`37 bp deleted → frameshift`
179.6	`GTCCTTAGGCCGCCAGGGGGCGCCGG----------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`16 bp deleted → frameshift`
173.1	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------AAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`11 bp deleted → frameshift`
170.8	`GTCCTTAGGCCGCCAGGGGGCGCCGG-----------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`17 bp deleted → frameshift`
163.8	`GTCCTTAGGCCGCCAGGG--------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`26 bp deleted → frameshift`
162.8	`GTCCTTAGGCCGCCAGGGGGCGCCGG------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`18 bp deleted → no frameshift`
156.6	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-------------GAAGCCGGGCCGGCCGCGTTATTACCATAA`	`13 bp deleted → frameshift`
155.4	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC---------------------------GGCCGCGTTATTACCATAA`	`27 bp deleted → no frameshift`
149.1	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA--------------AGCCGGGCCGGCCGCGTTATTACCATAA`	`14 bp deleted → frameshift`
140.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC---------------------GGGCCGGCCGCGTTATTACCATAA`	`21 bp deleted → no frameshift`
133.2	`GTCCTTAGGCCGCCAGGGGG----------------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`22 bp deleted → frameshift`
132.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------------TTATTACCATAA`	`36 bp deleted → no frameshift`
128.1	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-----------------CCGGGCCGGCCGCGTTATTACCATAA`	`17 bp deleted → frameshift`
127.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC-------------------------------GCGTTATTACCATAA`	`31 bp deleted → frameshift`
120.4	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC------------------------CGGGCCGGCCGCGTTATTACCATAA`	`24 bp deleted → no frameshift`
120.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------------TTATTACCATAA`	`38 bp deleted → frameshift`
115.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGC---------------------------------CGGCCGCGTTATTACCATAA`	`33 bp deleted → no frameshift`
114.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG-------------------------GGCCGGCCGCGTTATTACCATAA`	`25 bp deleted → frameshift`
114.8	`GTCCTTAGGCCGCCAGGGGGCGCCGG-------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`25 bp deleted → frameshift`
113.6	`GTCCTTAGGCCGCCAGGGGGC---------------------------------------CGGCCGCGTTATTACCATAA`	`39 bp deleted → no frameshift`
109.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGC----------------------------------GTTATTACCATAA`	`34 bp deleted → frameshift`
109.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC--------------------------GGCCGCGTTATTACCATAA`	`26 bp deleted → frameshift`
109.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGC--------------------------CGGGCCGGCCGCGTTATTACCATAA`	`26 bp deleted → frameshift`
103.6	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCG---------------------------GGCCGGCCGCGTTATTACCATAA`	`27 bp deleted → no frameshift`
103.6	`GTCCTTAGGCCGCCAGG---------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`27 bp deleted → no frameshift`
103.2	`GTCCTTAGGCCGCCAGGGGGCGCCG-----------------------------------------CGTTATTACCATAA`	`41 bp deleted → frameshift`
98.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGC----------------------------CGGGCCGGCCGCGTTATTACCATAA`	`28 bp deleted → frameshift`
94.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC-----------------------------CGGCCGCGTTATTACCATAA`	`29 bp deleted → frameshift`
94.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCG-----------------------------GGCCGGCCGCGTTATTACCATAA`	`29 bp deleted → frameshift`
91.5	`GTCCTTAGGCCGCCAGG----------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`34 bp deleted → frameshift`
89.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC------------------------------GCGTTATTACCATAA`	`30 bp deleted → no frameshift`
89.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------GCCGCGTTATTACCATAA`	`30 bp deleted → no frameshift`
84.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGC-------------------------------CGGCCGCGTTATTACCATAA`	`31 bp deleted → frameshift`
84.8	`GTCCTTAGGCCGCCAGGGGG-------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`31 bp deleted → frameshift`
84.0	`GTCCTTAGGCCG---------------------------------------------GGCCGGCCGCGTTATTACCATAA`	`45 bp deleted → no frameshift`
82.0	`GTCCTTAGGCCG--------------------------------------------------GCCGCGTTATTACCATAA`	`50 bp deleted → frameshift`
81.0	`GTCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------------TTATTACCATAA`	`40 bp deleted → frameshift`
81.0	`GTCCTTAGGCCGCCAGGGG----------------------------------------CCGGCCGCGTTATTACCATAA`	`40 bp deleted → frameshift`
80.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------GCCGCGTTATTACCATAA`	`32 bp deleted → frameshift`
80.8	`GTCCTTAGGCCGCCAGGGG--------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`32 bp deleted → frameshift`
80.4	`GTCCTTAGGCCGC------------------------------------------------------GTTATTACCATAA`	`54 bp deleted → no frameshift`
77.4	`GTCCTTAGGCCGCCAGGG-----------------------------------------CCGGCCGCGTTATTACCATAA`	`41 bp deleted → frameshift`
76.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC---------------------------------CGCGTTATTACCATAA`	`33 bp deleted → no frameshift`
76.8	`GTCCTTAGGCCGCCAGGG---------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`33 bp deleted → no frameshift`
76.8	`GTCCTTAGG---------------------------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`33 bp deleted → no frameshift`
73.2	`GTCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------GCCGCGTTATTACCATAA`	`34 bp deleted → frameshift`
73.2	`GTCCTTAGGCCGCCAGGGGGC----------------------------------CGGGCCGGCCGCGTTATTACCATAA`	`34 bp deleted → frameshift`
73.2	`GTCCTTAGG----------------------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`34 bp deleted → frameshift`
73.2	`GTCCTTAGGCCGCC------------------------------------------GGGCCGGCCGCGTTATTACCATAA`	`42 bp deleted → no frameshift`
69.6	`GTCCTTAGGCCGCCAGGGGGC-------------------------------------------CGCGTTATTACCATAA`	`43 bp deleted → frameshift`
69.6	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGC-----------------------------------CGCGTTATTACCATAA`	`35 bp deleted → frameshift`
69.6	`GTCCTTAGGCCGCCAGGGGGCG-----------------------------------GGCCGGCCGCGTTATTACCATAA`	`35 bp deleted → frameshift`
69.6	`GTCCTTAGG-----------------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`35 bp deleted → frameshift`
66.6	`GTCCTTAGGCCGCCAGGGGGCGC--------------------------------------------GTTATTACCATAA`	`44 bp deleted → frameshift`
64.5	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------------------------------------TAA`	`41 bp deleted → frameshift`
61.0	`GTCCTTAGG------------------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAA`	`42 bp deleted → no frameshift`
60.6	`GTCCTTAGGCCGCCA--------------------------------AAGGAAGCCGGGCCGGCCGCGTTATTACCATAA`	`32 bp deleted → frameshift`
60.0	`GTCCTTAGGCCGCCAGGGGGCG----------------------------------------------TTATTACCATAA`	`46 bp deleted → frameshift`
60.0	`GTCCTTAGGCCGCCAGGGGG--------------------------------------GCCGGCCGCGTTATTACCATAA`	`38 bp deleted → frameshift`
57.6	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT---------------------------------ACCATAA`	`33 bp deleted → no frameshift`
57.0	`GTCCTTAGGCCGCC-----------------------------------------------GGCCGCGTTATTACCATAA`	`47 bp deleted → frameshift`
54.0	`GTCCTTAGGCCGCCAGGGGGCG----------------------------------------GCCGCGTTATTACCATAA`	`40 bp deleted → frameshift`
48.8	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC------------------------------------------ATAA`	`42 bp deleted → no frameshift`
48.8	`GTCCTTAGGCCGCCAGG------------------------------------------CCGGCCGCGTTATTACCATAA`	`42 bp deleted → no frameshift`
46.8	`GTCCTTAGGCCGCC---------------------------------------------------GCGTTATTACCATAA`	`51 bp deleted → no frameshift`
46.4	`GTCCTTAGGCCGCCAGGGGGCGCCG-------------------------------------------TTATTACCATAA`	`43 bp deleted → frameshift`
45.0	`GTCCTTAGGCCGCCAG--------------------------------------CCGGGCCGGCCGCGTTATTACCATAA`	`38 bp deleted → frameshift`
44.6	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT------------------------------ATTACCATAA`	`30 bp deleted → no frameshift`
44.4	`GTCCTTAGGCCGCCAGGGG--------------------------------------------CCGCGTTATTACCATAA`	`44 bp deleted → frameshift`
42.0	`GTCCTTAGGCCGCCAGGG---------------------------------------------CCGCGTTATTACCATAA`	`45 bp deleted → no frameshift`
40.0	`GTCCTTAGGCCGCCAGG----------------------------------------------CCGCGTTATTACCATAA`	`46 bp deleted → frameshift`
34.4	`GTCCTTAGG-------------------------------------------------GCCGGCCGCGTTATTACCATAA`	`49 bp deleted → frameshift`
30.0	`GTCCTTAG----------------------------------------------CCGGGCCGGCCGCGTTATTACCATAA`	`46 bp deleted → frameshift`
29.6	`GTCCTTAGGCCGCCAGGGGGCGCC----------------------------------------------------ATAA`	`52 bp deleted → frameshift`
29.6	`GTCC----------------------------------------------------GGGCCGGCCGCGTTATTACCATAA`	`52 bp deleted → frameshift`
28.4	`GTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGAT---------------------------------------AA`	`39 bp deleted → no frameshift`
24.4	`GTCCTTAGGCCG--------------------------------------------------------TTATTACCATAA`	`56 bp deleted → frameshift`
23.2	`GTCCTTAGGC---------------------------------------------------------GTTATTACCATAA`	`57 bp deleted → no frameshift`
23.2	`GTCC---------------------------------------------------------GGCCGCGTTATTACCATAA`	`57 bp deleted → no frameshift`
22.5	`GTCCTTAGGCCGCCA--------------------------------------------------------------TAA`	`62 bp deleted → frameshift`
18.8	`GTCC-------------------------------------------------------------GCGTTATTACCATAA`	`61 bp deleted → frameshift`
15.6	`GTCCTTAGGCC-----------------------------------------------------------------ATAA`	`65 bp deleted → frameshift`
12.3	`GTCCTTA----------------------------------------------------------------TTACCATAA`	`64 bp deleted → frameshift`
10.8	`GTCC------------------------------------------------------------------------ATAA`	`72 bp deleted → no frameshift`
10.5	`GTCCTTA-------------------------------------------------------------------CCATAA`	`67 bp deleted → frameshift`
10.5	`GT-------------------------------------------------------------------TATTACCATAA`	`67 bp deleted → frameshift`
5.4	`GTCCTTA------------------------------------------------------------------------A`	`72 bp deleted → no frameshift`

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