CRISPOR

Micro-homology scoring of potential deletions

Guide: GGTGAGCTGCGAGAATAGCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	(Wild-type sequence)
314.3	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC----------------GAGGTCGCCCCCGCCCTGGCCACT`	`16 bp deleted → frameshift`
311.6	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-----GCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`5 bp deleted → frameshift`
298.2	`GCGGCGATATCATCATCCATGGTGAGCTGCG--------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`14 bp deleted → frameshift`
282.0	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-------GCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`7 bp deleted → frameshift`
269.4	`GCGGCGATATCATCATCCATGGTGAGCTGCG----------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`16 bp deleted → frameshift`
255.2	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC---------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`9 bp deleted → no frameshift`
247.0	`GCGGCGATATCATCATCCATGGTGAGC----------------------------CGAGGTCGCCCCCGCCCTGGCCACT`	`28 bp deleted → frameshift`
245.0	`GCGGCGATATCATCATCCATGGTGAGCTG---------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`21 bp deleted → no frameshift`
191.1	`GCGGCGATATCATCATCCATGGTGAGCTGCGAG--------------------------GTCGCCCCCGCCCTGGCCACT`	`26 bp deleted → frameshift`
175.0	`GCGGCGATATCATCATCCATGGTGAGCTGCGAG---------------------CCGAGGTCGCCCCCGCCCTGGCCACT`	`21 bp deleted → no frameshift`
172.2	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC-------------------------CCCGCCCTGGCCACT`	`25 bp deleted → frameshift`
170.8	`GCGGCGATATCATCATCCATGGTGAGC-----------------GCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`17 bp deleted → frameshift`
162.8	`GCGGCGATATCATCATCCATGGTGAGCTGC------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`18 bp deleted → no frameshift`
154.8	`GCGGCGATATCATCATCCATGGTGAGC-------------------GCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`19 bp deleted → frameshift`
147.2	`GCGGCGATATCATCATCCATGG--------------------GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`20 bp deleted → frameshift`
140.0	`GCGGCGATATCATCATCCATGG---------------------CGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`21 bp deleted → no frameshift`
127.2	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC-------------------------------CTGGCCACT`	`31 bp deleted → frameshift`
116.1	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGA-------------------GCCGAGGTCGCCCCCGCCCTGGCCACT`	`19 bp deleted → frameshift`
114.8	`GCGGCGATATCATCATCCATGGTGAGCTGC-------------------------CGAGGTCGCCCCCGCCCTGGCCACT`	`25 bp deleted → frameshift`
113.6	`GCGGCG---------------------------------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`39 bp deleted → no frameshift`
109.2	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC--------------------------CCGCCCTGGCCACT`	`26 bp deleted → frameshift`
105.0	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAG---------------------GTCGCCCCCGCCCTGGCCACT`	`21 bp deleted → no frameshift`
103.6	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC---------------------------CGCCCTGGCCACT`	`27 bp deleted → no frameshift`
98.8	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC----------------------------GCCCTGGCCACT`	`28 bp deleted → frameshift`
96.0	`GCGGCGATATCATCATCCATGGTGAG---------------------------------GTCGCCCCCGCCCTGGCCACT`	`33 bp deleted → no frameshift`
95.1	`GCGGCGATATCATCATCCATGGTGAGCTG-----------------------AGCCGAGGTCGCCCCCGCCCTGGCCACT`	`23 bp deleted → frameshift`
94.2	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC-------------------------------------ACT`	`37 bp deleted → frameshift`
90.3	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGA------------------------GGTCGCCCCCGCCCTGGCCACT`	`24 bp deleted → no frameshift`
81.9	`GCGGCGATATCATCATCCATGGTG--------------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`26 bp deleted → frameshift`
80.8	`GCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCC--------------------------------TGGCCACT`	`32 bp deleted → frameshift`
80.8	`GCGGCGATATCATCATCCATGGTGAGCTGCG--------------------------------CCCCCGCCCTGGCCACT`	`32 bp deleted → frameshift`
77.4	`GCGGCG-----------------------------------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`41 bp deleted → frameshift`
75.0	`GCGGCGATATCATCATCCATGGT--------------------------------------CGCCCCCGCCCTGGCCACT`	`38 bp deleted → frameshift`
73.2	`GCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------CCCCGCCCTGGCCACT`	`34 bp deleted → frameshift`
73.2	`GCG------------------------------------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`42 bp deleted → no frameshift`
70.5	`GCGGCGATATCATCATCCATG-----------------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`29 bp deleted → frameshift`
66.6	`GCG--------------------------------------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`44 bp deleted → frameshift`
63.6	`GCGGCGATATCATCATCCATG-------------------------------AGCCGAGGTCGCCCCCGCCCTGGCCACT`	`31 bp deleted → frameshift`
62.8	`GCGGCGATATCATCATCCATGGTGAGC-------------------------------------CCCCGCCCTGGCCACT`	`37 bp deleted → frameshift`
60.0	`GCGGCGATATCATCATCCATGGTGAGCTGCG--------------------------------------CCCTGGCCACT`	`38 bp deleted → frameshift`
60.0	`GCGGCGATATCATCATCC--------------------------------------GAGGTCGCCCCCGCCCTGGCCACT`	`38 bp deleted → frameshift`
60.0	`GCGG--------------------------------------GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`38 bp deleted → frameshift`
54.0	`GCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------------CCTGGCCACT`	`40 bp deleted → frameshift`
52.5	`GCGGCGATATCATCATCCATGGTGAGCTG---------------------------------------------GCCACT`	`45 bp deleted → no frameshift`
46.4	`GCGGCGATATCATCATCCATGGTGAGC-------------------------------------------CCTGGCCACT`	`43 bp deleted → frameshift`
46.4	`GCGGC-------------------------------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`43 bp deleted → frameshift`
40.0	`GCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------------------CACT`	`46 bp deleted → frameshift`
40.0	`GC----------------------------------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCACT`	`46 bp deleted → frameshift`
39.0	`GCGGCGA---------------------------------------------------GGTCGCCCCCGCCCTGGCCACT`	`51 bp deleted → no frameshift`
38.0	`GCGGCGATATCATCATCC-----------------------------------------------CCCGCCCTGGCCACT`	`47 bp deleted → frameshift`
36.4	`GCGGCGATATCATCATCC------------------------------------------------CCGCCCTGGCCACT`	`48 bp deleted → no frameshift`
35.5	`GCGGCGATATCATCATCCATGG-----------------------------------------------------CCACT`	`53 bp deleted → frameshift`
34.4	`GCGGCGATATCATCATCCATGGTGAGC-------------------------------------------------CACT`	`49 bp deleted → frameshift`
34.4	`GCGGCGATATCATCATCC-------------------------------------------------CGCCCTGGCCACT`	`49 bp deleted → frameshift`
32.8	`GCGGCGATATCATCATCC--------------------------------------------------GCCCTGGCCACT`	`50 bp deleted → frameshift`
32.8	`GCGGC--------------------------------------------------CGAGGTCGCCCCCGCCCTGGCCACT`	`50 bp deleted → frameshift`
31.5	`GCGGCGATATCATCATC---------------------------------------------GCCCCCGCCCTGGCCACT`	`45 bp deleted → no frameshift`
30.0	`GCGGCGA----------------------------------------------GCCGAGGTCGCCCCCGCCCTGGCCACT`	`46 bp deleted → frameshift`
28.4	`GCGGCGATATCATCATCC-----------------------------------------------------CTGGCCACT`	`53 bp deleted → frameshift`
28.4	`GC-----------------------------------------------------CGAGGTCGCCCCCGCCCTGGCCACT`	`53 bp deleted → frameshift`
27.3	`GCGGCGATATCATC------------------------------------------------GCCCCCGCCCTGGCCACT`	`48 bp deleted → no frameshift`
26.8	`GCGGCGATATCATCATCC------------------------------------------------------TGGCCACT`	`54 bp deleted → no frameshift`
26.8	`GCG------------------------------------------------------AGGTCGCCCCCGCCCTGGCCACT`	`54 bp deleted → no frameshift`
26.0	`GCGGCGATATCATCATCCA-----------------------------------------------------------CT`	`59 bp deleted → frameshift`
24.6	`GCGGCGATATCATCATCCATGGTG--------------------------------------------------GCCACT`	`50 bp deleted → frameshift`
24.4	`GCGG--------------------------------------------------------TCGCCCCCGCCCTGGCCACT`	`56 bp deleted → frameshift`
23.4	`GCGGCGATATC---------------------------------------------------GCCCCCGCCCTGGCCACT`	`51 bp deleted → no frameshift`
23.2	`GCGGCG---------------------------------------------------------CCCCCGCCCTGGCCACT`	`57 bp deleted → no frameshift`
22.2	`GCGGCGATATCATCATCCATGGTGAGCT----------------------------------------------------`	`52 bp deleted → frameshift`
20.8	`GCGGC-----------------------------------------------------------CCCCGCCCTGGCCACT`	`59 bp deleted → frameshift`
20.0	`GCG------------------------------------------------------------CCCCCGCCCTGGCCACT`	`60 bp deleted → no frameshift`
18.0	`GC--------------------------------------------------------------CCCCGCCCTGGCCACT`	`62 bp deleted → frameshift`
17.4	`GCGGC-----------------------------------------------------------------------CACT`	`71 bp deleted → frameshift`
17.2	`GCGGCG---------------------------------------------------------------CCCTGGCCACT`	`63 bp deleted → no frameshift`
15.6	`GCGGC-----------------------------------------------------------------CCTGGCCACT`	`65 bp deleted → frameshift`
14.8	`GCG------------------------------------------------------------------CCCTGGCCACT`	`66 bp deleted → no frameshift`
13.2	`GC--------------------------------------------------------------------CCTGGCCACT`	`68 bp deleted → frameshift`
12.9	`GCGGCGATATCATCA---------------------------------------------------------------CT`	`63 bp deleted → no frameshift`
11.1	`GCGGCGATATCA------------------------------------------------------------------CT`	`66 bp deleted → no frameshift`
10.0	`GC--------------------------------------------------------------------------CACT`	`74 bp deleted → frameshift`

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