CRISPOR

Micro-homology scoring of potential deletions

Guide: TTCCGGCGCGCCGAGTCCTT AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	(Wild-type sequence)
417.6	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG-------------CCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`13 bp deleted → frameshift`
359.2	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCC----------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`16 bp deleted → frameshift`
329.4	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGAC`	`34 bp deleted → frameshift`
327.6	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGAC`	`26 bp deleted → frameshift`
282.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC-------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`7 bp deleted → frameshift`
256.2	`GTCGCCCCCGCCCTGGCCACTTCCGGC-----------------CGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`17 bp deleted → frameshift`
247.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGC----------------------------CGGCGCGCGCCCAGATTGGGGAC`	`28 bp deleted → frameshift`
244.2	`GTCGCCCCCGCCCTGGCCACTTCCGGCGC------------------CAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`18 bp deleted → no frameshift`
242.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC----------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`10 bp deleted → frameshift`
231.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCC------------------------------------CAGATTGGGGAC`	`36 bp deleted → no frameshift`
211.5	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAG-------GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`7 bp deleted → frameshift`
210.0	`GTCGCCCCCGCCCTGGCCACTTCCG---------------------CCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`21 bp deleted → no frameshift`
207.2	`GTCGCCCCCGCCCTGGCC---------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`27 bp deleted → no frameshift`
188.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGC---------------CGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`15 bp deleted → no frameshift`
165.0	`GTCGCCCCCGCC------------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`36 bp deleted → no frameshift`
161.6	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGAC`	`32 bp deleted → frameshift`
156.1	`GTCGCCCCCGCCCTGGCCA------------------------------GGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`30 bp deleted → no frameshift`
147.2	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC--------------------GGCGCGCGCCCAGATTGGGGAC`	`20 bp deleted → frameshift`
147.2	`GTCGCCCCCGCCCTGGCCACTTCCGGC--------------------CAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`20 bp deleted → frameshift`
141.6	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAG---------------GGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`15 bp deleted → no frameshift`
126.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG-----------------------CCGGCGCGCGCCCAGATTGGGGAC`	`23 bp deleted → frameshift`
120.4	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGC------------------------GCCGGCGCGCGCCCAGATTGGGGAC`	`24 bp deleted → no frameshift`
120.4	`GTCGCCCCCGCCCTGGCCACTTCC------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`24 bp deleted → no frameshift`
120.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGC--------------------------------------CCAGATTGGGGAC`	`38 bp deleted → frameshift`
115.2	`GTCGCCCCCGCC---------------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`33 bp deleted → no frameshift`
114.8	`GTCGCCCCCGCCCTGGCCACTTCCGG-------------------------GGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`25 bp deleted → frameshift`
109.2	`GTCGCCCCCGCCCTGGCCACTTCCGG--------------------------GGCGCCGGCGCGCGCCCAGATTGGGGAC`	`26 bp deleted → frameshift`
103.6	`GTCGCCCCCGCCCTGGCCACTTCCGG---------------------------GCGCCGGCGCGCGCCCAGATTGGGGAC`	`27 bp deleted → no frameshift`
97.6	`GTCGCC------------------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`42 bp deleted → no frameshift`
94.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGAC`	`29 bp deleted → frameshift`
94.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGAC`	`29 bp deleted → frameshift`
90.0	`GTCGCCCCCGCCCTGGC--------------------------------------GCCGGCGCGCGCCCAGATTGGGGAC`	`38 bp deleted → frameshift`
89.2	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGAC`	`30 bp deleted → no frameshift`
89.2	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGAC`	`30 bp deleted → no frameshift`
89.2	`GTCGCCCCCGCCCTGGCCACTTCCGGC------------------------------CGGCGCGCGCCCAGATTGGGGAC`	`30 bp deleted → no frameshift`
85.2	`GTCGCC---------------------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`39 bp deleted → no frameshift`
84.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGAC`	`31 bp deleted → frameshift`
84.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGAC`	`31 bp deleted → frameshift`
84.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGAC`	`31 bp deleted → frameshift`
84.8	`GTCGCCCCCGCCCTGGCCACTTCCG-------------------------------CCGGCGCGCGCCCAGATTGGGGAC`	`31 bp deleted → frameshift`
81.0	`GTCGCCCCCGCCCTGGCC----------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`40 bp deleted → frameshift`
80.8	`GTCGCCCCCGCCC--------------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`32 bp deleted → frameshift`
80.0	`GTCGCCCCCGCC----------------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`46 bp deleted → frameshift`
76.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGAC`	`33 bp deleted → no frameshift`
69.6	`GTCGCCCCCGCCCTGG-----------------------------------GGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`35 bp deleted → frameshift`
69.6	`GTCGCCCCCGCCC-----------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`35 bp deleted → frameshift`
66.6	`GTCGCCCCCGCCCTGGC--------------------------------------------GCGCGCCCAGATTGGGGAC`	`44 bp deleted → frameshift`
66.0	`GTCGCCCCCGCCCTGG------------------------------------GGCGCCGGCGCGCGCCCAGATTGGGGAC`	`36 bp deleted → no frameshift`
62.8	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCC-------------------------------------AGATTGGGGAC`	`37 bp deleted → frameshift`
62.8	`GTCGCCCCCGCCCTGGCCACTTCCG-------------------------------------CGCGCCCAGATTGGGGAC`	`37 bp deleted → frameshift`
62.8	`GTCGCCCCCGCCCTGG-------------------------------------GCGCCGGCGCGCGCCCAGATTGGGGAC`	`37 bp deleted → frameshift`
62.8	`GTCGCCCC-------------------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`37 bp deleted → frameshift`
61.0	`GTCGCCCCCGCCC--------------------------------------------------------AGATTGGGGAC`	`56 bp deleted → frameshift`
60.0	`GTCGCCC--------------------------------------GCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`38 bp deleted → frameshift`
59.2	`GTCGCC----------------------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`52 bp deleted → frameshift`
56.8	`GTCGCCCCCGCCCTGGCCACTTCCG---------------------------------------CGCCCAGATTGGGGAC`	`39 bp deleted → no frameshift`
56.8	`GTCGCCCCC---------------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`39 bp deleted → no frameshift`
54.0	`GTCGCCCCCGCCCTGGCCACTTCCGGC----------------------------------------CCAGATTGGGGAC`	`40 bp deleted → frameshift`
54.0	`GTCGCCCC----------------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`40 bp deleted → frameshift`
51.6	`GTCGCCCCCGCCCTGGCCACTTCCG-----------------------------------------CCCAGATTGGGGAC`	`41 bp deleted → frameshift`
51.6	`GTCGCCC-----------------------------------------AGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC`	`41 bp deleted → frameshift`
51.6	`GTCGCCCCCG-------------------------------------------------GCGCGCGCCCAGATTGGGGAC`	`49 bp deleted → frameshift`
49.5	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAG------------------------------------ATTGGGGAC`	`36 bp deleted → no frameshift`
49.2	`GTCGCCCCCGCCCTGGCC--------------------------------------------------CAGATTGGGGAC`	`50 bp deleted → frameshift`
45.0	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGA--------------------------------------TTGGGGAC`	`38 bp deleted → frameshift`
45.0	`GTCGCCC--------------------------------------------------------------AGATTGGGGAC`	`62 bp deleted → frameshift`
44.4	`GTCGCCCCCGCCCTGGCCACTTCC--------------------------------------------CAGATTGGGGAC`	`44 bp deleted → frameshift`
44.4	`GTCGCCCCCGC--------------------------------------------GCCGGCGCGCGCCCAGATTGGGGAC`	`44 bp deleted → frameshift`
44.4	`GTCGCCCCCGC----------------------------------------------------GCGCCCAGATTGGGGAC`	`52 bp deleted → frameshift`
42.0	`GTCGCCCCCGCCCTGGCCACTTCC---------------------------------------------AGATTGGGGAC`	`45 bp deleted → no frameshift`
42.0	`GTCGCCCCCGCCC---------------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`45 bp deleted → no frameshift`
40.2	`GTCGCCCCCGC------------------------------------------------------GCCCAGATTGGGGAC`	`54 bp deleted → no frameshift`
40.0	`GTCGCCCCCGCCCTGGC----------------------------------------------GCGCCCAGATTGGGGAC`	`46 bp deleted → frameshift`
39.0	`GTCGCCCCCGCCCTGGCCA---------------------------------------------------GATTGGGGAC`	`51 bp deleted → no frameshift`
36.6	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGAC`	`34 bp deleted → frameshift`
36.4	`GTCGCCCCCGCCCTGGC------------------------------------------------GCCCAGATTGGGGAC`	`48 bp deleted → no frameshift`
33.0	`GTCGC----------------------------------------------------------GCGCCCAGATTGGGGAC`	`58 bp deleted → frameshift`
32.8	`GTCGCCCCCGCCCTGGCCACTTCCGG--------------------------------------------------GGAC`	`50 bp deleted → frameshift`
32.8	`GTCGCCCCCGC--------------------------------------------------GCGCGCCCAGATTGGGGAC`	`50 bp deleted → frameshift`
32.8	`GTCGCCCC--------------------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`50 bp deleted → frameshift`
32.8	`GTCGC--------------------------------------------------GCCGGCGCGCGCCCAGATTGGGGAC`	`50 bp deleted → frameshift`
31.5	`GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGA---------------------------------------------C`	`45 bp deleted → no frameshift`
31.2	`GTCGCCCCCGCCCTGGCCACTTCCGG---------------------------------------------------GAC`	`51 bp deleted → no frameshift`
31.2	`GTCGCCC---------------------------------------------------GGCGCGCGCCCAGATTGGGGAC`	`51 bp deleted → no frameshift`
30.0	`GTCGCCCCC------------------------------------------------------------AGATTGGGGAC`	`60 bp deleted → no frameshift`
30.0	`GTCGC------------------------------------------------------------GCCCAGATTGGGGAC`	`60 bp deleted → no frameshift`
29.6	`GTCGCCCCCGCCCTGGCCACTTCCGG----------------------------------------------------AC`	`52 bp deleted → frameshift`
28.2	`GTCGCCCC-------------------------------------------------------------AGATTGGGGAC`	`61 bp deleted → frameshift`
25.6	`GTCGCCCCCGCCC-------------------------------------------------------CAGATTGGGGAC`	`55 bp deleted → frameshift`
25.6	`GTCG-------------------------------------------------------GCGCGCGCCCAGATTGGGGAC`	`55 bp deleted → frameshift`
25.0	`GTCGCCCCCGCCCTGG------------------------------------------------------------GGAC`	`60 bp deleted → no frameshift`
24.4	`GTCGC--------------------------------------------------------GCGCGCCCAGATTGGGGAC`	`56 bp deleted → frameshift`
23.2	`GTCGCCCCCGCC---------------------------------------------------------AGATTGGGGAC`	`57 bp deleted → no frameshift`
20.8	`GTCGCCCCC-----------------------------------------------------------CAGATTGGGGAC`	`59 bp deleted → frameshift`
18.8	`GTCGCCCCCGCCCTGG-------------------------------------------------------------GAC`	`61 bp deleted → frameshift`
18.0	`GTCGCCCCCGCCCTGG--------------------------------------------------------------AC`	`62 bp deleted → frameshift`
17.2	`GTCGCC---------------------------------------------------------------AGATTGGGGAC`	`63 bp deleted → no frameshift`
15.0	`GTCGCCCCCGCCCTGGCCAC------------------------------------------------------------`	`60 bp deleted → no frameshift`
14.8	`GTCGCCCCCGCCCTGGCCACTT----------------------------------------------------GGGGAC`	`52 bp deleted → frameshift`

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