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Contents:

PCR primers for off-targets of GCCGGGCGCGCTGTGAGCCG AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529674_F TCGTCGGCAGCGTCCCATGGTGAGCTGCGAGAAT 60.4 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529674_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr16 1.17 Mbp_chr16_1165723_F TCGTCGGCAGCGTCCCTGAGGATGTAGAGGGGCT 60.1 0.45
mm4__chr16 1.17 Mbp_chr16_1165723_R GTCTCGTGGGCTCGGGTTCATCTGGTGGGGAACGT 59.9 0.45
mm4__chr19 40.21 Mbp_chr19_40214127_F Primer3: not found at this Tm N.d. 0.43
mm4__chr19 40.21 Mbp_chr19_40214127_R Primer3: not found at this Tm N.d. 0.43
mm4__chr5 138.33 Mbp_chr5_138326406_F Primer3: not found at this Tm N.d. 0.41
mm4__chr5 138.33 Mbp_chr5_138326406_R Primer3: not found at this Tm N.d. 0.41
mm4__chr6 89.76 Mbp_chr6_89756850_F Primer3: not found at this Tm N.d. 0.40
mm4__chr6 89.76 Mbp_chr6_89756850_R Primer3: not found at this Tm N.d. 0.40
mm4__chr17 77.45 Mbp_chr17_77451481_F TCGTCGGCAGCGTCAAGCCCCTTTGATCCTCTGC 60.0 0.40
mm4__chr17 77.45 Mbp_chr17_77451481_R GTCTCGTGGGCTCGGTGCAAGGCTGGGAAGAACAA 60.1 0.40
mm4__chr5 79.18 Mbp_chr5_79180281_F Primer3: not found at this Tm N.d. 0.37
mm4__chr5 79.18 Mbp_chr5_79180281_R Primer3: not found at this Tm N.d. 0.37
mm4__chr20 41.33 Mbp_chr20_41325974_F Primer3: not found at this Tm N.d. 0.37
mm4__chr20 41.33 Mbp_chr20_41325974_R Primer3: not found at this Tm N.d. 0.37
mm4__chr2 34.12 Mbp_chr2_34116603_F Primer3: not found at this Tm N.d. 0.37
mm4__chr2 34.12 Mbp_chr2_34116603_R Primer3: not found at this Tm N.d. 0.37
mm4__chr15 98.59 Mbp_chr15_98585068_F Primer3: not found at this Tm N.d. 0.37
mm4__chr15 98.59 Mbp_chr15_98585068_R Primer3: not found at this Tm N.d. 0.37
mm4__chr20 2.57 Mbp_chr20_2573625_F Primer3: not found at this Tm N.d. 0.37
mm4__chr20 2.57 Mbp_chr20_2573625_R Primer3: not found at this Tm N.d. 0.37
mm4__chr10 60.18 Mbp_chr10_60177662_F Primer3: not found at this Tm N.d. 0.37
mm4__chr10 60.18 Mbp_chr10_60177662_R Primer3: not found at this Tm N.d. 0.37
mm4__chr5 73.63 Mbp_chr5_73626581_F TCGTCGGCAGCGTCGAGCGGCAACAAGTGGAGA 60.3 0.37
mm4__chr5 73.63 Mbp_chr5_73626581_R GTCTCGTGGGCTCGGGACACTGGCGGGGCTTTC 61.0 0.37
mm4__chr1 178.62 Mbp_chr1_178615137_F Primer3: not found at this Tm N.d. 0.37
mm4__chr1 178.62 Mbp_chr1_178615137_R Primer3: not found at this Tm N.d. 0.37
mm4__chr3 45.73 Mbp_chr3_45730580_F Primer3: not found at this Tm N.d. 0.34
mm4__chr3 45.73 Mbp_chr3_45730580_R Primer3: not found at this Tm N.d. 0.34
mm4__chr9 127.49 Mbp_chr9_127489949_F TCGTCGGCAGCGTCCAGCTCAGACAGACAGAGGC 60.1 0.32
mm4__chr9 127.49 Mbp_chr9_127489949_R GTCTCGTGGGCTCGGTCACTTTTCAGCAGGCGTCA 60.1 0.32
mm4__chr1 167.72 Mbp_chr1_167722325_F Primer3: not found at this Tm N.d. 0.31
mm4__chr1 167.72 Mbp_chr1_167722325_R Primer3: not found at this Tm N.d. 0.31
mm4__chr2 19.02 Mbp_chr2_19022477_F TCGTCGGCAGCGTCTGACACCAGGAAAACTGTGCT 60.0 0.31
mm4__chr2 19.02 Mbp_chr2_19022477_R GTCTCGTGGGCTCGGGGTGTTTCGCAACATGACCC 60.0 0.31
mm4__chr13 110.45 Mbp_chr13_110452198_F Primer3: not found at this Tm N.d. 0.30
mm4__chr13 110.45 Mbp_chr13_110452198_R Primer3: not found at this Tm N.d. 0.30
mm4__chr16 84.16 Mbp_chr16_84159153_F TCGTCGGCAGCGTCCCTCCTCCCTGGTCCTAAGT 59.6 0.30
mm4__chr16 84.16 Mbp_chr16_84159153_R GTCTCGTGGGCTCGGCGCTAGACAGAGAAGCAGCA 59.8 0.30
mm4__chr20 29.65 Mbp_chr20_29651057_F Primer3: not found at this Tm N.d. 0.29
mm4__chr20 29.65 Mbp_chr20_29651057_R Primer3: not found at this Tm N.d. 0.29
mm4__chr14 104.86 Mbp_chr14_104864999_F Primer3: not found at this Tm N.d. 0.28
mm4__chr14 104.86 Mbp_chr14_104864999_R Primer3: not found at this Tm N.d. 0.28
mm4__chr12 131.56 Mbp_chr12_131560518_F Primer3: not found at this Tm N.d. 0.25
mm4__chr12 131.56 Mbp_chr12_131560518_R Primer3: not found at this Tm N.d. 0.25
mm4__chr12 131.07 Mbp_chr12_131070643_F Primer3: not found at this Tm N.d. 0.25
mm4__chr12 131.07 Mbp_chr12_131070643_R Primer3: not found at this Tm N.d. 0.25
mm4__chr6 41.33 Mbp_chr6_41334858_F TCGTCGGCAGCGTCATGGAGTGGAGAGCAGGAGT 59.9 0.24
mm4__chr6 41.33 Mbp_chr6_41334858_R GTCTCGTGGGCTCGGCTGCTGGACTGGCGATCTG 60.5 0.24
mm4__chr11 114.06 Mbp_chr11_114060521_F TCGTCGGCAGCGTCGAGGGAGGGAAGCTCCAGA 59.9 0.24
mm4__chr11 114.06 Mbp_chr11_114060521_R GTCTCGTGGGCTCGGCATCAGGTGCAGAGACCGG 60.1 0.24
mm3__chr18 79.02 Mbp_chr18_79022655_F TCGTCGGCAGCGTCCCGGAAAGTGTTGCTCTCCT 59.9 0.23
mm3__chr18 79.02 Mbp_chr18_79022655_R GTCTCGTGGGCTCGGCACTGGCTTTCTCGTGGGTC 60.9 0.23
mm4__chr13 113.11 Mbp_chr13_113109818_F TCGTCGGCAGCGTCGGGGTCACTCTCCACCACC 61.5 0.23
mm4__chr13 113.11 Mbp_chr13_113109818_R GTCTCGTGGGCTCGGCTCTTCAGGACGCCCTACAG 59.5 0.23
mm4__chr2 75.61 Mbp_chr2_75611861_F TCGTCGGCAGCGTCCCTCTCCAGTGCCTCATGTC 59.8 0.23
mm4__chr2 75.61 Mbp_chr2_75611861_R GTCTCGTGGGCTCGGTTGATCTGTCCCAGCAGCAG 60.0 0.23
mm4__chr12 24.22 Mbp_chr12_24216413_F Primer3: not found at this Tm N.d. 0.21
mm4__chr12 24.22 Mbp_chr12_24216413_R Primer3: not found at this Tm N.d. 0.21
mm4__chrX 78.10 Mbp_chrX_78104156_F TCGTCGGCAGCGTCCCAGGGAGCAATGGCAGC 61.1 0.21
mm4__chrX 78.10 Mbp_chrX_78104156_R GTCTCGTGGGCTCGGGAACAGGGCCCACACTACC 60.0 0.21
mm4__chr2 112.68 Mbp_chr2_112683039_F Primer3: not found at this Tm N.d. 0.21
mm4__chr2 112.68 Mbp_chr2_112683039_R Primer3: not found at this Tm N.d. 0.21
mm4__chr8 28.62 Mbp_chr8_28622678_F Primer3: not found at this Tm N.d. 0.20
mm4__chr8 28.62 Mbp_chr8_28622678_R Primer3: not found at this Tm N.d. 0.20
mm4__chr11 126.45 Mbp_chr11_126452827_F TCGTCGGCAGCGTCAAGCCAGGTCTTTTGGGGAG 59.8 0.20
mm4__chr11 126.45 Mbp_chr11_126452827_R GTCTCGTGGGCTCGGCAGGAGGGGAAGAAAGACCG 59.7 0.20
mm4__chr12 6.28 Mbp_chr12_6275771_F Primer3: not found at this Tm N.d. 0.20
mm4__chr12 6.28 Mbp_chr12_6275771_R Primer3: not found at this Tm N.d. 0.20
mm4__chr16 58.20 Mbp_chr16_58198178_F Primer3: not found at this Tm N.d. 0.20
mm4__chr16 58.20 Mbp_chr16_58198178_R Primer3: not found at this Tm N.d. 0.20
mm3__chr16 2.47 Mbp_chr16_2468137_F TCGTCGGCAGCGTCCTTCTGACCGCCGCCTTC 60.8 0.18
mm3__chr16 2.47 Mbp_chr16_2468137_R GTCTCGTGGGCTCGGCTCGCGGTGACCTCGATG 60.2 0.18
mm4__chr2 240.58 Mbp_chr2_240575194_F TCGTCGGCAGCGTCACTTTCTCCGAGTGAGCAGC 60.0 0.17
mm4__chr2 240.58 Mbp_chr2_240575194_R GTCTCGTGGGCTCGGCAGTTCCACCCCAACTGCC 60.9 0.17
mm3__chr18 79.55 Mbp_chr18_79548767_F Primer3: not found at this Tm N.d. 0.17
mm3__chr18 79.55 Mbp_chr18_79548767_R Primer3: not found at this Tm N.d. 0.17
mm3__chr21 42.84 Mbp_chr21_42837679_F TCGTCGGCAGCGTCCAGCACCTTCTCCACCCG 60.0 0.16
mm3__chr21 42.84 Mbp_chr21_42837679_R GTCTCGTGGGCTCGGGCTTTCAGGGCCATGTCCT 60.0 0.16
mm4__chr21 43.66 Mbp_chr21_43659492_F TCGTCGGCAGCGTCCCTGAATTTTGGCAACCGAA 57.1 0.15
mm4__chr21 43.66 Mbp_chr21_43659492_R GTCTCGTGGGCTCGGCTGATGGCGTCACAAAGGC 59.4 0.15
mm4__chr7 157.84 Mbp_chr7_157835614_F TCGTCGGCAGCGTCAGGAAACAGGGATCTGCAGC 60.0 0.15
mm4__chr7 157.84 Mbp_chr7_157835614_R GTCTCGTGGGCTCGGTTTCCCACGCATTTGCAGTG 59.9 0.15
mm4__chr12 5.53 Mbp_chr12_5525463_F TCGTCGGCAGCGTCAGGCAGAAACCACAGGACTG 59.8 0.14
mm4__chr12 5.53 Mbp_chr12_5525463_R GTCTCGTGGGCTCGGCTTGTCCCCAACAACAGCAG 59.3 0.14
mm2__chr1 20.55 Mbp_chr1_20553949_F Primer3: not found at this Tm N.d. 0.14
mm2__chr1 20.55 Mbp_chr1_20553949_R Primer3: not found at this Tm N.d. 0.14
mm4__chr17 50.15 Mbp_chr17_50150943_F Primer3: not found at this Tm N.d. 0.14
mm4__chr17 50.15 Mbp_chr17_50150943_R Primer3: not found at this Tm N.d. 0.14
mm4__chr6 168.37 Mbp_chr6_168370454_F TCGTCGGCAGCGTCGCTTTGGACAGCATCACCAC 59.7 0.13
mm4__chr6 168.37 Mbp_chr6_168370454_R GTCTCGTGGGCTCGGGGGCACAGACAAGGAGAAGG 60.3 0.13
mm4__chr15 69.05 Mbp_chr15_69046258_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15 69.05 Mbp_chr15_69046258_R Primer3: not found at this Tm N.d. 0.13
mm4__chr17 81.29 Mbp_chr17_81288302_F TCGTCGGCAGCGTCAACACCATTTCCTCTCCCGC 60.3 0.13
mm4__chr17 81.29 Mbp_chr17_81288302_R GTCTCGTGGGCTCGGTGTTAGGTCCTCCCTGCAGA 59.8 0.13
mm4__chr17 80.15 Mbp_chr17_80152144_F Primer3: not found at this Tm N.d. 0.13
mm4__chr17 80.15 Mbp_chr17_80152144_R Primer3: not found at this Tm N.d. 0.13
mm4__chr20 63.88 Mbp_chr20_63879004_F Primer3: not found at this Tm N.d. 0.13
mm4__chr20 63.88 Mbp_chr20_63879004_R Primer3: not found at this Tm N.d. 0.13
mm4__chr13 21.05 Mbp_chr13_21054337_F Primer3: not found at this Tm N.d. 0.12
mm4__chr13 21.05 Mbp_chr13_21054337_R Primer3: not found at this Tm N.d. 0.12
mm4__chr20 24.38 Mbp_chr20_24380901_F Primer3: not found at this Tm N.d. 0.11
mm4__chr20 24.38 Mbp_chr20_24380901_R Primer3: not found at this Tm N.d. 0.11
mm4__chr12 40.01 Mbp_chr12_40008540_F Primer3: not found at this Tm N.d. 0.11
mm4__chr12 40.01 Mbp_chr12_40008540_R Primer3: not found at this Tm N.d. 0.11
mm4__chr5 132.49 Mbp_chr5_132490377_F Primer3: not found at this Tm N.d. 0.11
mm4__chr5 132.49 Mbp_chr5_132490377_R Primer3: not found at this Tm N.d. 0.11
mm3__chr11 68.21 Mbp_chr11_68213419_F Primer3: not found at this Tm N.d. 0.10
mm3__chr11 68.21 Mbp_chr11_68213419_R Primer3: not found at this Tm N.d. 0.10
mm4__chr4 3.46 Mbp_chr4_3461474_F TCGTCGGCAGCGTCTCTGGGGAAACTTCTGGCTA 57.9 0.10
mm4__chr4 3.46 Mbp_chr4_3461474_R GTCTCGTGGGCTCGGGCTGAAAGGTGGAGGAAGCT 59.9 0.10
mm4__chr20 10.07 Mbp_chr20_10070346_F TCGTCGGCAGCGTCTCTGAGCAATCCTCCCCTCT 59.6 0.10
mm4__chr20 10.07 Mbp_chr20_10070346_R GTCTCGTGGGCTCGGTCCCAGTTGCTTATAACCCACC 60.0 0.10
mm4__chr13 113.40 Mbp_chr13_113399997_F TCGTCGGCAGCGTCCTGTTGTCACTTGCACCCAC 59.6 0.09
mm4__chr13 113.40 Mbp_chr13_113399997_R GTCTCGTGGGCTCGGGACTCGTTGACTGCCTCCTC 60.1 0.09
mm4__chr18 76.41 Mbp_chr18_76412967_F TCGTCGGCAGCGTCCCTACCCGGAACCTACCAGA 60.0 0.09
mm4__chr18 76.41 Mbp_chr18_76412967_R GTCTCGTGGGCTCGGCCTCACAGAGGCCTTGGTTT 59.8 0.09
mm4__chr2 598.29 Kbp_chr2_598288_F TCGTCGGCAGCGTCCCCTCGGGTGATTCAGGACT 60.9 0.09
mm4__chr2 598.29 Kbp_chr2_598288_R GTCTCGTGGGCTCGGCGTGAGTGACCACATCCCTC 60.1 0.09
mm3__chr19 46.49 Mbp_chr19_46493426_F Primer3: not found at this Tm N.d. 0.08
mm3__chr19 46.49 Mbp_chr19_46493426_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 149.10 Mbp_chr1_149103259_F TCGTCGGCAGCGTCCAACAAAGCTTGCGACAGCC 60.9 0.08
mm4__chr1 149.10 Mbp_chr1_149103259_R GTCTCGTGGGCTCGGTTACAGCGAAGTCCACCCAG 59.6 0.08
mm4__chr1 145.43 Mbp_chr1_145425311_F TCGTCGGCAGCGTCCAACAAAGCTTGCGACAGCC 60.9 0.08
mm4__chr1 145.43 Mbp_chr1_145425311_R GTCTCGTGGGCTCGGTTACAGCGAAGTCCACCCAG 59.6 0.08
mm4__chr1 144.46 Mbp_chr1_144461369_F TCGTCGGCAGCGTCAACAAAACTTGCGACAGCCG 60.2 0.08
mm4__chr1 144.46 Mbp_chr1_144461369_R GTCTCGTGGGCTCGGTTACAGCGAAGTCCACCCAG 59.6 0.08
mm4__chr14 106.58 Mbp_chr14_106580503_F Primer3: not found at this Tm N.d. 0.08
mm4__chr14 106.58 Mbp_chr14_106580503_R Primer3: not found at this Tm N.d. 0.08
mm4__chr17 63.70 Mbp_chr17_63700018_F TCGTCGGCAGCGTCCCTCACCCCACACCCCTC 61.3 0.08
mm4__chr17 63.70 Mbp_chr17_63700018_R GTCTCGTGGGCTCGGCCCTCCTCGTACCCGTGC 61.8 0.08
mm4__chr13 30.42 Mbp_chr13_30421676_F Primer3: not found at this Tm N.d. 0.08
mm4__chr13 30.42 Mbp_chr13_30421676_R Primer3: not found at this Tm N.d. 0.08
mm4__chr19 23.12 Mbp_chr19_23117334_F TCGTCGGCAGCGTCCAGTCCCCTTCAGCCATACG 60.1 0.08
mm4__chr19 23.12 Mbp_chr19_23117334_R GTCTCGTGGGCTCGGCAGTGCAGGGAAGAGACAGG 60.0 0.08
mm4__chr8 37.80 Mbp_chr8_37797353_F TCGTCGGCAGCGTCGATGGGTTGATGGGCGCC 61.2 0.08
mm4__chr8 37.80 Mbp_chr8_37797353_R GTCTCGTGGGCTCGGCTGCGGATGGATAGCGGG 60.0 0.08
mm4__chr9 113.94 Mbp_chr9_113944065_F TCGTCGGCAGCGTCCTGGATGGGGAACGTGATGG 60.4 0.08
mm4__chr9 113.94 Mbp_chr9_113944065_R GTCTCGTGGGCTCGGTCCACATTGGATCAAACCGT 57.4 0.08
mm4__chr4 171.81 Mbp_chr4_171813063_F Primer3: not found at this Tm N.d. 0.07
mm4__chr4 171.81 Mbp_chr4_171813063_R Primer3: not found at this Tm N.d. 0.07
mm4__chr3 127.02 Mbp_chr3_127017994_F Primer3: not found at this Tm N.d. 0.07
mm4__chr3 127.02 Mbp_chr3_127017994_R Primer3: not found at this Tm N.d. 0.07
mm4__chr1 3.07 Mbp_chr1_3070724_F TCGTCGGCAGCGTCGGTTTTCCGAAGCTCCGCT 60.6 0.07
mm4__chr1 3.07 Mbp_chr1_3070724_R GTCTCGTGGGCTCGGACAAACTCGGGAGCTGCTTT 60.1 0.07
mm4__chr10 120.59 Mbp_chr10_120589443_F TCGTCGGCAGCGTCGTTAGTCTGCGAGTCCCAGC 60.4 0.07
mm4__chr10 120.59 Mbp_chr10_120589443_R GTCTCGTGGGCTCGGCCAGGGCTTAGTGTCCATCC 59.8 0.07
mm4__chr6 170.30 Mbp_chr6_170295866_F TCGTCGGCAGCGTCGGTCGTTTTGCGGTGGGG 61.6 0.06
mm4__chr6 170.30 Mbp_chr6_170295866_R GTCTCGTGGGCTCGGCGCCCGTTCTCATGCAAATC 60.2 0.06
mm4__chr5 159.05 Mbp_chr5_159051601_F TCGTCGGCAGCGTCACAACAACACAAACACCGCC 60.1 0.06
mm4__chr5 159.05 Mbp_chr5_159051601_R GTCTCGTGGGCTCGGGGCATTGTGGGGACTCATGA 60.0 0.06
mm4__chr11 19.71 Mbp_chr11_19712793_F TCGTCGGCAGCGTCCCACACCCTCTTTGCAGTGA 60.1 0.06
mm4__chr11 19.71 Mbp_chr11_19712793_R GTCTCGTGGGCTCGGGGGGAGGACAGGCTGCTG 62.1 0.06
mm4__chr16 4.39 Mbp_chr16_4392711_F TCGTCGGCAGCGTCCCACTAGGCAGGAAGGAGGA 60.3 0.06
mm4__chr16 4.39 Mbp_chr16_4392711_R GTCTCGTGGGCTCGGGCCCACACCTGGCTTCTATT 60.0 0.06
mm3__chr15 67.82 Mbp_chr15_67823619_F Primer3: not found at this Tm N.d. 0.05
mm3__chr15 67.82 Mbp_chr15_67823619_R Primer3: not found at this Tm N.d. 0.05
mm4__chr14 105.25 Mbp_chr14_105248684_F Primer3: not found at this Tm N.d. 0.05
mm4__chr14 105.25 Mbp_chr14_105248684_R Primer3: not found at this Tm N.d. 0.05
mm4__chr9 135.69 Mbp_chr9_135685666_F Primer3: not found at this Tm N.d. 0.05
mm4__chr9 135.69 Mbp_chr9_135685666_R Primer3: not found at this Tm N.d. 0.05
mm4__chr9 135.69 Mbp_chr9_135685650_F Primer3: not found at this Tm N.d. 0.05
mm4__chr9 135.69 Mbp_chr9_135685650_R Primer3: not found at this Tm N.d. 0.05
mm4__chr4 849.53 Kbp_chr4_849530_F TCGTCGGCAGCGTCGCTTTCTCTTCATGTGCCTGG 59.5 0.05
mm4__chr4 849.53 Kbp_chr4_849530_R GTCTCGTGGGCTCGGCAGCTCCACAGGTTGGGAG 60.0 0.05
mm4__chr19 36.15 Mbp_chr19_36151609_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 36.15 Mbp_chr19_36151609_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 69.21 Mbp_chr2_69211893_F TCGTCGGCAGCGTCGCTATTTCAGCCTACTCACCA 57.4 0.04
mm4__chr2 69.21 Mbp_chr2_69211893_R GTCTCGTGGGCTCGGACGAGAGGAGTGGGTTGACT 60.1 0.04
mm4__chr19 19.26 Mbp_chr19_19261517_F TCGTCGGCAGCGTCCTGTGAGCAGCAGGACGC 61.1 0.04
mm4__chr19 19.26 Mbp_chr19_19261517_R GTCTCGTGGGCTCGGAGGCCTCCGAAAAGCCAAG 60.3 0.04
mm3__chr14 76.39 Mbp_chr14_76388243_F Primer3: not found at this Tm N.d. 0.04
mm3__chr14 76.39 Mbp_chr14_76388243_R Primer3: not found at this Tm N.d. 0.04
mm4__chr3 44.58 Mbp_chr3_44581193_F Primer3: not found at this Tm N.d. 0.04
mm4__chr3 44.58 Mbp_chr3_44581193_R Primer3: not found at this Tm N.d. 0.04
mm4__chr17 17.04 Mbp_chr17_17042647_F Primer3: not found at this Tm N.d. 0.04
mm4__chr17 17.04 Mbp_chr17_17042647_R Primer3: not found at this Tm N.d. 0.04
mm3__chr2 238.24 Mbp_chr2_238239475_F TCGTCGGCAGCGTCCGTCTGGCAGGGGTTACAG 60.0 0.04
mm3__chr2 238.24 Mbp_chr2_238239475_R GTCTCGTGGGCTCGGCAAGCTGGAGAACGCCGAA 60.6 0.04
mm4__chr9 136.20 Mbp_chr9_136199065_F Primer3: not found at this Tm N.d. 0.04
mm4__chr9 136.20 Mbp_chr9_136199065_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 24.08 Mbp_chr14_24081336_F Primer3: not found at this Tm N.d. 0.03
mm4__chr14 24.08 Mbp_chr14_24081336_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 2.82 Mbp_chr1_2816637_F Primer3: not found at this Tm N.d. 0.03
mm4__chr1 2.82 Mbp_chr1_2816637_R Primer3: not found at this Tm N.d. 0.03
mm3__chr10 131.97 Mbp_chr10_131971154_F TCGTCGGCAGCGTCCCCAGAGGCACATGTCAGTG 60.6 0.03
mm3__chr10 131.97 Mbp_chr10_131971154_R GTCTCGTGGGCTCGGAGGTGATCTGTCCCTGGAGG 60.3 0.03
mm4__chrX 40.16 Mbp_chrX_40155017_F Primer3: not found at this Tm N.d. 0.03
mm4__chrX 40.16 Mbp_chrX_40155017_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 175.19 Mbp_chr1_175192692_F TCGTCGGCAGCGTCAAACTCACCTTTCGGTCCGC 60.8 0.03
mm4__chr1 175.19 Mbp_chr1_175192692_R GTCTCGTGGGCTCGGAAAAGGAACCCACACCTCCC 59.8 0.03
mm4__chr20 63.83 Mbp_chr20_63830993_F Primer3: not found at this Tm N.d. 0.03
mm4__chr20 63.83 Mbp_chr20_63830993_R Primer3: not found at this Tm N.d. 0.03
mm4__chr12 52.02 Mbp_chr12_52015051_F Primer3: not found at this Tm N.d. 0.03
mm4__chr12 52.02 Mbp_chr12_52015051_R Primer3: not found at this Tm N.d. 0.03
mm4__chr17 73.19 Mbp_chr17_73192612_F Primer3: not found at this Tm N.d. 0.03
mm4__chr17 73.19 Mbp_chr17_73192612_R Primer3: not found at this Tm N.d. 0.03
mm4__chr5 180.33 Mbp_chr5_180328579_F TCGTCGGCAGCGTCAATTCCACGGTGAGCGATGT 60.0 0.03
mm4__chr5 180.33 Mbp_chr5_180328579_R GTCTCGTGGGCTCGGGAAGCCTGGTCCCAGCTATC 59.8 0.03
mm3__chrX 71.43 Mbp_chrX_71430313_F Primer3: not found at this Tm N.d. 0.03
mm3__chrX 71.43 Mbp_chrX_71430313_R Primer3: not found at this Tm N.d. 0.03
mm3__chr5 65.55 Mbp_chr5_65547729_F Primer3: not found at this Tm N.d. 0.03
mm3__chr5 65.55 Mbp_chr5_65547729_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 64.42 Mbp_chr11_64419552_F TCGTCGGCAGCGTCCGGTCCAGACTCCTAGAGCT 60.1 0.03
mm4__chr11 64.42 Mbp_chr11_64419552_R GTCTCGTGGGCTCGGCCTAGCCTGGGTGACATGAC 59.8 0.03
mm3__chr7 66.24 Mbp_chr7_66244013_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 66.24 Mbp_chr7_66244013_R Primer3: not found at this Tm N.d. 0.03
mm3__chr13 21.27 Mbp_chr13_21267309_F Primer3: not found at this Tm N.d. 0.03
mm3__chr13 21.27 Mbp_chr13_21267309_R Primer3: not found at this Tm N.d. 0.03
mm3__chr12 85.67 Mbp_chr12_85668081_F Primer3: not found at this Tm N.d. 0.03
mm3__chr12 85.67 Mbp_chr12_85668081_R Primer3: not found at this Tm N.d. 0.03
mm4__chr12 131.71 Mbp_chr12_131710788_F Primer3: not found at this Tm N.d. 0.03
mm4__chr12 131.71 Mbp_chr12_131710788_R Primer3: not found at this Tm N.d. 0.03
mm3__chr11 66.46 Mbp_chr11_66456789_F Primer3: not found at this Tm N.d. 0.02
mm3__chr11 66.46 Mbp_chr11_66456789_R Primer3: not found at this Tm N.d. 0.02
mm4__chr8 144.48 Mbp_chr8_144478115_F TCGTCGGCAGCGTCCCAGCGGACCCACTTGTG 60.6 0.02
mm4__chr8 144.48 Mbp_chr8_144478115_R GTCTCGTGGGCTCGGCTCTCCCTCGCTCCGCTC 61.5 0.02
mm3__chr2 77.44 Mbp_chr2_77438471_F Primer3: not found at this Tm N.d. 0.02
mm3__chr2 77.44 Mbp_chr2_77438471_R Primer3: not found at this Tm N.d. 0.02
mm4__chr2 200.51 Mbp_chr2_200509939_F Primer3: not found at this Tm N.d. 0.02
mm4__chr2 200.51 Mbp_chr2_200509939_R Primer3: not found at this Tm N.d. 0.02
mm4__chr12 68.81 Mbp_chr12_68808006_F TCGTCGGCAGCGTCTAAAGGAGTGTCACAGCGCC 60.3 0.02
mm4__chr12 68.81 Mbp_chr12_68808006_R GTCTCGTGGGCTCGGTCCGGGATGATGGAGTGGG 60.7 0.02
mm4__chr21 14.06 Mbp_chr21_14064609_F TCGTCGGCAGCGTCGACAAGCAGCAAAGGTAGCG 59.8 0.02
mm4__chr21 14.06 Mbp_chr21_14064609_R GTCTCGTGGGCTCGGCAGCTCAGGGTCCGTTTGG 60.6 0.02
mm4__chr21 13.98 Mbp_chr21_13979596_F TCGTCGGCAGCGTCAAACAGCTGAGGGTCCGTTT 59.8 0.02
mm4__chr21 13.98 Mbp_chr21_13979596_R GTCTCGTGGGCTCGGAAGCAGCACAGGTAGCGG 60.0 0.02
mm4__chr18 14.18 Mbp_chr18_14179679_F TCGTCGGCAGCGTCAAGCAGCACAGGTAGCGAG 60.0 0.02
mm4__chr18 14.18 Mbp_chr18_14179679_R GTCTCGTGGGCTCGGAAACAGCTGAGGGTCCGTTT 59.8 0.02
mm2__chr1 115.64 Mbp_chr1_115642028_F TCGTCGGCAGCGTCTCTGCCTCTCCAGGAGCC 60.3 0.02
mm2__chr1 115.64 Mbp_chr1_115642028_R GTCTCGTGGGCTCGGCCTCTCGCGGGACTCACC 61.8 0.02
mm4__chr22 20.70 Mbp_chr22_20700511_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22 20.70 Mbp_chr22_20700511_R Primer3: not found at this Tm N.d. 0.01
mm3__chr6 82.00 Mbp_chr6_82004682_F Primer3: not found at this Tm N.d. 0.01
mm3__chr6 82.00 Mbp_chr6_82004682_R Primer3: not found at this Tm N.d. 0.01
mm4__chr7 100.90 Mbp_chr7_100895922_F TCGTCGGCAGCGTCGAGCCGACTCTGACAGCC 59.8 0.01
mm4__chr7 100.90 Mbp_chr7_100895922_R GTCTCGTGGGCTCGGGGAGAGAGAGGGGAGGTGG 60.0 0.01
mm4__chr9 128.69 Mbp_chr9_128689851_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 128.69 Mbp_chr9_128689851_R Primer3: not found at this Tm N.d. 0.01
mm3__chr1 36.22 Mbp_chr1_36224421_F TCGTCGGCAGCGTCTACATTTCCCAGGAGGCAGC 59.7 0.01
mm3__chr1 36.22 Mbp_chr1_36224421_R GTCTCGTGGGCTCGGCCTACCACAGAGATCGCAGC 60.2 0.01
mm3__chr16 71.69 Mbp_chr16_71692134_F Primer3: not found at this Tm N.d. 0.01
mm3__chr16 71.69 Mbp_chr16_71692134_R Primer3: not found at this Tm N.d. 0.01
mm4__chr7 71.13 Mbp_chr7_71132015_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 71.13 Mbp_chr7_71132015_R Primer3: not found at this Tm N.d. 0.01
mm4__chr5 140.65 Mbp_chr5_140647381_F TCGTCGGCAGCGTCTTCAGCTCCACGTAGCGTTT 59.9 0.01
mm4__chr5 140.65 Mbp_chr5_140647381_R GTCTCGTGGGCTCGGACCTCGACCTCCCTGAAGTT 60.1 0.01
mm4__chr2 100.82 Mbp_chr2_100820362_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 100.82 Mbp_chr2_100820362_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 79.69 Mbp_chr12_79691243_F Primer3: not found at this Tm N.d. 0.01
mm4__chr12 79.69 Mbp_chr12_79691243_R Primer3: not found at this Tm N.d. 0.01
mm4__chr8 102.81 Mbp_chr8_102810891_F Primer3: not found at this Tm N.d. 0.01
mm4__chr8 102.81 Mbp_chr8_102810891_R Primer3: not found at this Tm N.d. 0.01
mm4__chr3 75.47 Mbp_chr3_75474010_F Primer3: not found at this Tm N.d. 0.01
mm4__chr3 75.47 Mbp_chr3_75474010_R Primer3: not found at this Tm N.d. 0.01
mm4__chr2 120.46 Mbp_chr2_120463678_F TCGTCGGCAGCGTCGAGACGGAAAGGAAGTGCCA 59.9 0.01
mm4__chr2 120.46 Mbp_chr2_120463678_R GTCTCGTGGGCTCGGCCATAGACGCATCGTTCCCA 59.8 0.01
mm4__chr9 137.46 Mbp_chr9_137459124_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 137.46 Mbp_chr9_137459124_R Primer3: not found at this Tm N.d. 0.01
mm4__chr8 143.72 Mbp_chr8_143716159_F Primer3: not found at this Tm N.d. 0.01
mm4__chr8 143.72 Mbp_chr8_143716159_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 1.01 Mbp_chr19_1007778_F TCGTCGGCAGCGTCTCGCCATGCTCACGTGAG 60.1 0.01
mm4__chr19 1.01 Mbp_chr19_1007778_R GTCTCGTGGGCTCGGTGTCCATGATGAAGGCGTTGA 59.9 0.01
mm4__chr22 37.52 Mbp_chr22_37519730_F TCGTCGGCAGCGTCCGACACACCTTCCCCGGG 62.3 0.01
mm4__chr22 37.52 Mbp_chr22_37519730_R GTCTCGTGGGCTCGGAGCCCAGCTTGAGTGCCC 62.3 0.01
mm4__chr11 79.44 Mbp_chr11_79439916_F TCGTCGGCAGCGTCCCTCAGTTGGCTCCTAGACG 59.5 0.01
mm4__chr11 79.44 Mbp_chr11_79439916_R GTCTCGTGGGCTCGGGGTGGTTGGAGCTCAGATCC 60.1 0.01
mm4__chr14 105.31 Mbp_chr14_105314828_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 105.31 Mbp_chr14_105314828_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 13.00 Mbp_chr19_12996534_F TCGTCGGCAGCGTCGGCGGGGCTCCCAAATTT 60.6 0.00
mm4__chr19 13.00 Mbp_chr19_12996534_R GTCTCGTGGGCTCGGGTTTGCGCCGAGGAAAGTTT 59.9 0.00
mm4__chr10 44.39 Mbp_chr10_44385148_F TCGTCGGCAGCGTCCGCTTTAGAGGGGAGAGCCA 61.3 0.00
mm4__chr10 44.39 Mbp_chr10_44385148_R GTCTCGTGGGCTCGGGAGTCGCGCGTCAGAGAC 60.5 0.00
mm4__chrX 41.47 Mbp_chrX_41473974_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 41.47 Mbp_chrX_41473974_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 141.18 Mbp_chrX_141177134_F TCGTCGGCAGCGTCGAGCCCTCGCAGGAAGTG 60.1 0.00
mm4__chrX 141.18 Mbp_chrX_141177134_R GTCTCGTGGGCTCGGGTCCGTCCGAATGGCCTC 60.2 0.00
mm4__chr9 95.51 Mbp_chr9_95511135_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 95.51 Mbp_chr9_95511135_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 137.20 Mbp_chr9_137200177_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 137.20 Mbp_chr9_137200177_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 136.81 Mbp_chr9_136808449_F TCGTCGGCAGCGTCCTTGCCGGTTGTGGGGTG 61.2 0.00
mm4__chr9 136.81 Mbp_chr9_136808449_R GTCTCGTGGGCTCGGGAGTGCGGCTGGGAAACG 61.4 0.00
mm4__chr9 135.70 Mbp_chr9_135702072_F TCGTCGGCAGCGTCGATCGCCCTCCCTACCCC 60.9 0.00
mm4__chr9 135.70 Mbp_chr9_135702072_R GTCTCGTGGGCTCGGAGGAAAAGACGCCAGTTCCA 59.5 0.00
mm4__chr9 124.00 Mbp_chr9_123999897_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 124.00 Mbp_chr9_123999897_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 19.76 Mbp_chr8_19757137_F TCGTCGGCAGCGTCGGGCTGTGGGGATCTGCC 62.5 0.00
mm4__chr8 19.76 Mbp_chr8_19757137_R GTCTCGTGGGCTCGGCTTCGGGTGAGTCGGGTC 59.4 0.00
mm4__chr8 144.11 Mbp_chr8_144108533_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 144.11 Mbp_chr8_144108533_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 10.73 Mbp_chr8_10730157_F TCGTCGGCAGCGTCCGAAGAGGGCCCTCGTCC 61.8 0.00
mm4__chr8 10.73 Mbp_chr8_10730157_R GTCTCGTGGGCTCGGGCAAGATGCTGGGTGAGTGA 60.3 0.00
mm4__chr7 74.29 Mbp_chr7_74289645_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 74.29 Mbp_chr7_74289645_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 44.11 Mbp_chr7_44110090_F TCGTCGGCAGCGTCAGATCCGAGCCTCCTCCATG 60.8 0.00
mm4__chr7 44.11 Mbp_chr7_44110090_R GTCTCGTGGGCTCGGCGGAGGAGTCCCTTATCCCA 60.1 0.00
mm4__chr7 35.26 Mbp_chr7_35258403_F TCGTCGGCAGCGTCTCCAGGCCTTGTAGCGTCTT 61.1 0.00
mm4__chr7 35.26 Mbp_chr7_35258403_R GTCTCGTGGGCTCGGGATGAACGGGGAGCGAGTG 60.5 0.00
mm4__chr7 29.99 Mbp_chr7_29989467_F TCGTCGGCAGCGTCAGGCCACCTCTCCTCTGAC 60.3 0.00
mm4__chr7 29.99 Mbp_chr7_29989467_R GTCTCGTGGGCTCGGCCACCTCCTGCGTTTCATCT 60.0 0.00
mm4__chr7 2.64 Mbp_chr7_2641827_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 2.64 Mbp_chr7_2641827_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 149.15 Mbp_chr7_149147675_F TCGTCGGCAGCGTCCCGCCTGTGGAGAGGACC 61.7 0.00
mm4__chr7 149.15 Mbp_chr7_149147675_R GTCTCGTGGGCTCGGATGGCCCTGCGCTGTCTA 61.0 0.00
mm4__chr6 89.41 Mbp_chr6_89412166_F TCGTCGGCAGCGTCTCTAGAGCTCAGCGGGGC 60.8 0.00
mm4__chr6 89.41 Mbp_chr6_89412166_R GTCTCGTGGGCTCGGCTCCGGCTGACTCCTCCG 61.5 0.00
mm4__chr6 149.86 Mbp_chr6_149863556_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 149.86 Mbp_chr6_149863556_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 56.05 Mbp_chr4_56049088_F TCGTCGGCAGCGTCGGAGCATGTGACCACGCC 61.1 0.00
mm4__chr4 56.05 Mbp_chr4_56049088_R GTCTCGTGGGCTCGGCTCCATTGCTCTCCCCACTG 60.1 0.00
mm4__chr4 4.86 Mbp_chr4_4860195_F TCGTCGGCAGCGTCCCAAGGAGAGCGCCCTGG 62.4 0.00
mm4__chr4 4.86 Mbp_chr4_4860195_R GTCTCGTGGGCTCGGCCACCGAGAAATGGCCGAG 60.8 0.00
mm4__chr3 128.49 Mbp_chr3_128489251_F TCGTCGGCAGCGTCCCCGATTCGCCAGGAAATGA 60.4 0.00
mm4__chr3 128.49 Mbp_chr3_128489251_R GTCTCGTGGGCTCGGCTGAACTGGCCTGGGAGG 59.3 0.00
mm4__chr3 128.24 Mbp_chr3_128243815_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 128.24 Mbp_chr3_128243815_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 126.08 Mbp_chr3_126084284_F TCGTCGGCAGCGTCGACCTCGGACGACTTGGC 60.1 0.00
mm4__chr3 126.08 Mbp_chr3_126084284_R GTCTCGTGGGCTCGGCTCGCGAGACTCGGTCCC 61.8 0.00
mm4__chr3 101.85 Mbp_chr3_101849797_F TCGTCGGCAGCGTCCCTGAGCTACTTCTACGGCG 59.9 0.00
mm4__chr3 101.85 Mbp_chr3_101849797_R GTCTCGTGGGCTCGGGACGAGGCAGAGGAGAAGTC 59.5 0.00
mm4__chr22 50.19 Mbp_chr22_50194265_F TCGTCGGCAGCGTCCAGTTCTCAGAACCCAGGAGG 59.7 0.00
mm4__chr22 50.19 Mbp_chr22_50194265_R GTCTCGTGGGCTCGGCTCCAGGAGCAGGTTGAAGG 60.0 0.00
mm4__chr22 45.95 Mbp_chr22_45952558_F TCGTCGGCAGCGTCAACCGAGCTGGGGAAATGAG 60.0 0.00
mm4__chr22 45.95 Mbp_chr22_45952558_R GTCTCGTGGGCTCGGCTGAGCCTGCTGGGAGAATT 59.7 0.00
mm4__chr22 41.49 Mbp_chr22_41485727_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 41.49 Mbp_chr22_41485727_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 19.76 Mbp_chr22_19760589_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 19.76 Mbp_chr22_19760589_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 36.71 Mbp_chr21_36708069_F TCGTCGGCAGCGTCCAGGGGTCGGGAGCTAGG 60.8 0.00
mm4__chr21 36.71 Mbp_chr21_36708069_R GTCTCGTGGGCTCGGCAGACCCTCCACTAAGCGC 60.1 0.00
mm4__chr21 36.70 Mbp_chr21_36700862_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 36.70 Mbp_chr21_36700862_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 6.77 Mbp_chr20_6767974_F Primer3: not found at this Tm N.d. 0.00
mm4__chr20 6.77 Mbp_chr20_6767974_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 241.54 Mbp_chr2_241541850_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 241.54 Mbp_chr2_241541850_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 240.57 Mbp_chr2_240568331_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 240.57 Mbp_chr2_240568331_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 240.56 Mbp_chr2_240557845_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 240.56 Mbp_chr2_240557845_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 166.38 Mbp_chr2_166376021_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 166.38 Mbp_chr2_166376021_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 149.04 Mbp_chr2_149038728_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 149.04 Mbp_chr2_149038728_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 48.50 Mbp_chr19_48499739_F TCGTCGGCAGCGTCGGAACGGTGAGGAGAGGGTC 61.6 0.00
mm4__chr19 48.50 Mbp_chr19_48499739_R GTCTCGTGGGCTCGGTGCAGCTCACCTACTTGCTC 60.0 0.00
mm4__chr19 33.62 Mbp_chr19_33622702_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 33.62 Mbp_chr19_33622702_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 2.34 Mbp_chr19_2340129_F TCGTCGGCAGCGTCGGTCATCGGGATCTTCTGCC 60.2 0.00
mm4__chr19 2.34 Mbp_chr19_2340129_R GTCTCGTGGGCTCGGCATCACCGCACAGAGTCAGG 60.7 0.00
mm4__chr19 19.12 Mbp_chr19_19117514_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 19.12 Mbp_chr19_19117514_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 10.55 Mbp_chr19_10552967_F TCGTCGGCAGCGTCCTCAGGACCACAGCCTGGA 60.9 0.00
mm4__chr19 10.55 Mbp_chr19_10552967_R GTCTCGTGGGCTCGGCCCCTCATCCCCTCCCTTTA 60.0 0.00
mm4__chr18 3.01 Mbp_chr18_3012809_F TCGTCGGCAGCGTCGGGTTCAGAGTCCTCAGCAG 59.7 0.00
mm4__chr18 3.01 Mbp_chr18_3012809_R GTCTCGTGGGCTCGGGTAGACGCGGCCCCTACC 61.9 0.00
mm4__chr17 82.09 Mbp_chr17_82090374_F TCGTCGGCAGCGTCAAGGCAGCCTCCTTTCTTGG 60.2 0.00
mm4__chr17 82.09 Mbp_chr17_82090374_R GTCTCGTGGGCTCGGGGAGGGGACAGTGCATCAAA 59.9 0.00
mm4__chr17 79.86 Mbp_chr17_79862053_F TCGTCGGCAGCGTCCTGAGTCGATGTGATGGCGT 60.1 0.00
mm4__chr17 79.86 Mbp_chr17_79862053_R GTCTCGTGGGCTCGGTGACCTTTCTGCTCAGCCAG 59.9 0.00
mm4__chr17 72.47 Mbp_chr17_72465826_F TCGTCGGCAGCGTCTCCCTGCGCTTGTTCCTATG 60.1 0.00
mm4__chr17 72.47 Mbp_chr17_72465826_R GTCTCGTGGGCTCGGAGGCAGGGACAGAGAAGAGT 59.8 0.00
mm4__chr17 15.94 Mbp_chr17_15944674_F TCGTCGGCAGCGTCGAGACAGCTCCGGTGGTG 59.7 0.00
mm4__chr17 15.94 Mbp_chr17_15944674_R GTCTCGTGGGCTCGGGAGCTCACTCCAGGCTAACC 59.8 0.00
mm4__chr17 1.52 Mbp_chr17_1516872_F TCGTCGGCAGCGTCCAACACTGCCCCAGACGAC 60.9 0.00
mm4__chr17 1.52 Mbp_chr17_1516872_R GTCTCGTGGGCTCGGAAGAGACAACTTCCGCCGG 60.0 0.00
mm4__chr16 88.79 Mbp_chr16_88785438_F TCGTCGGCAGCGTCCTCCCCTCCCGCAGTCTT 60.6 0.00
mm4__chr16 88.79 Mbp_chr16_88785438_R GTCTCGTGGGCTCGGGACCCCTTATGTAGCGCCC 59.9 0.00
mm4__chr16 48.39 Mbp_chr16_48385474_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 48.39 Mbp_chr16_48385474_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 22.81 Mbp_chr16_22814857_F TCGTCGGCAGCGTCCGGCCAGAAACTTCTCCAGA 59.6 0.00
mm4__chr16 22.81 Mbp_chr16_22814857_R GTCTCGTGGGCTCGGCTTGGGTGAGCCGGAGTG 60.0 0.00
mm4__chr15 79.09 Mbp_chr15_79091679_F TCGTCGGCAGCGTCCACGGCATTTATCCGAGAACG 59.7 0.00
mm4__chr15 79.09 Mbp_chr15_79091679_R GTCTCGTGGGCTCGGCGAGCTCGCTGCCCTCTG 62.8 0.00
mm4__chr15 64.05 Mbp_chr15_64046432_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 64.05 Mbp_chr15_64046432_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135991_F TCGTCGGCAGCGTCAGGAATGGTTGTGCCTGCTG 60.8 0.00
mm4__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135991_R GTCTCGTGGGCTCGGAGCAGGGGCAGATGCAAG 60.0 0.00
mm4__chr13 23.95 Mbp_chr13_23945645_F TCGTCGGCAGCGTCGGGATGGTAAGCAGCTGAGG 60.1 0.00
mm4__chr13 23.95 Mbp_chr13_23945645_R GTCTCGTGGGCTCGGTCAGCCCAGGGTGAGAGG 60.2 0.00
mm4__chr12 120.10 Mbp_chr12_120101002_F TCGTCGGCAGCGTCAGAGATGCCAAGAAACGCCT 59.6 0.00
mm4__chr12 120.10 Mbp_chr12_120101002_R GTCTCGTGGGCTCGGCCCTGACTTCATCCCCAGAC 59.4 0.00
mm4__chr11 44.07 Mbp_chr11_44066188_F TCGTCGGCAGCGTCACCGCCCTCAGTCTCCTAG 60.0 0.00
mm4__chr11 44.07 Mbp_chr11_44066188_R GTCTCGTGGGCTCGGACGTGTAGGTTTTCTCCGCC 60.3 0.00
mm4__chr11 33.38 Mbp_chr11_33376427_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 33.38 Mbp_chr11_33376427_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 119.47 Mbp_chr11_119470752_F TCGTCGGCAGCGTCCGGCCCATTTGTAGAGCTCA 60.1 0.00
mm4__chr11 119.47 Mbp_chr11_119470752_R GTCTCGTGGGCTCGGGCAGGCATCAGAGAGTCTGG 60.1 0.00
mm4__chr11 1.34 Mbp_chr11_1336928_F TCGTCGGCAGCGTCCCTCAGGTGTCTCGAGATGG 59.2 0.00
mm4__chr11 1.34 Mbp_chr11_1336928_R GTCTCGTGGGCTCGGCAGGAGGAGGCTCTAGCGTA 60.1 0.00
mm4__chr10 988.31 Kbp_chr10_988313_F TCGTCGGCAGCGTCCTCTCCTCTGTACCCTCGCT 60.1 0.00
mm4__chr10 988.31 Kbp_chr10_988313_R GTCTCGTGGGCTCGGCAATCGTGTTCTGCGTGCTC 60.1 0.00
mm4__chr10 133.47 Mbp_chr10_133467592_F TCGTCGGCAGCGTCCATGAGGGCAGAGTCAGTGG 60.1 0.00
mm4__chr10 133.47 Mbp_chr10_133467592_R GTCTCGTGGGCTCGGGCTGAGTGAGCTGCAGCATA 60.4 0.00
mm4__chr1 84.51 Mbp_chr1_84506775_F TCGTCGGCAGCGTCGGCATTTTCAGTTCCACCCC 59.3 0.00
mm4__chr1 84.51 Mbp_chr1_84506775_R GTCTCGTGGGCTCGGCCCTTCTCCAGCGAATCAGC 60.8 0.00
mm4__chr1 210.33 Mbp_chr1_210329288_F TCGTCGGCAGCGTCGAAGAAGACAAAAGCGGCGG 60.1 0.00
mm4__chr1 210.33 Mbp_chr1_210329288_R GTCTCGTGGGCTCGGGAAGCCAAGGAGAGAGGACG 59.8 0.00
mm4__chr1 193.93 Mbp_chr1_193927175_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 193.93 Mbp_chr1_193927175_R Primer3: not found at this Tm N.d. 0.00
mm3__chr8 101.21 Mbp_chr8_101206184_F TCGTCGGCAGCGTCGCTGAGTCACATTCCCGGAG 60.4 0.00
mm3__chr8 101.21 Mbp_chr8_101206184_R GTCTCGTGGGCTCGGGGCGGCTGTGAAAGAACAAA 59.6 0.00
mm3__chr19 33.19 Mbp_chr19_33194408_F Primer3: not found at this Tm N.d. 0.00
mm3__chr19 33.19 Mbp_chr19_33194408_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529674 CCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTC
CTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr16 1.17 Mbp_chr16_1165723 CCTGAGGATGTAGAGGGGCTGCCTCCCTGCCCCCACCCCGGCTCACAGTGCGGGTGGCTCCCGGCTCTCCTGAGTTGTGC
TGGCCACCTCACGTTCCCCACCAGATGAAC
mm4__chr17 77.45 Mbp_chr17_77451481 AAGCCCCTTTGATCCTCTGCTCGGCTCTGAGCCATGTGACCCGGTGGGCGGGCCGCGGCTCTCGGCGCGTCCAGCGCAGC
CCGACGTTCCGCTGCTGGGGTGAGTCCTGCTCCTTTGTTCTTCCCAGCCTTGCA
mm4__chr5 73.63 Mbp_chr5_73626581 GAGCGGCAACAAGTGGAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTCGCTCGGGCGCACTG
AGAGCCGGGGGCTGGGCGGGTGAAAGCCCCGCCAGTGTC
mm4__chr9 127.49 Mbp_chr9_127489949 CAGCTCAGACAGACAGAGGCGTCTCAGGGGCATATAGTATTTCCCCCAGAAGAGGGCAGGGGGAGCTGTGAGCCACGGTG
GTCCTGACGCCTGCTGAAAAGTGA
mm4__chr2 19.02 Mbp_chr2_19022477 TGACACCAGGAAAACTGTGCTCTCATATACTTATCCGCTGAGGCCAGGAGAGCTGTGAGACGGGGAAAAATAGTGAACAA
ATGTTACTTCTACTTTCCTGGGTCATGTTGCGAAACACC
mm4__chr16 84.16 Mbp_chr16_84159153 CCTCCTCCCTGGTCCTAAGTGACAGCGGGGAGAGCTGTAAGCCGAGGAAGAAGGGCCGTCCATCCTGCGGCACTCAGCAG
AGGTAAGCGGGCACTCCCCATCCTTTCCCTTAGTCACTGCTGCTTCTCTGTCTAGCG
mm4__chr6 41.33 Mbp_chr6_41334858 ATGGAGTGGAGAGCAGGAGTCCAGGGGCAGGCAAAGCAGTGGACTTGGAATCGAGTCCACTTGACAAGTTCCAATTCCCG
TCTCAGTTATTACCTCTGTTCACAGAGCTCCCGGCAGCAGATCGCCAGTCCAGCAG
mm4__chr11 114.06 Mbp_chr11_114060521 GAGGGAGGGAAGCTCCAGAGGGTCTGCAGCGCTGCGGGCCCTCCTCGGCTCTCGGCGGGACCGGCGGTGACACCGGAGCT
CGCCGTGCGCTCCCGGCCGCTCTCGGTGGGTGCCGGTCTCTGCACCTGATG
mm3__chr18 79.02 Mbp_chr18_79022655 CCGGAAAGTGTTGCTCTCCTTTCTATTGTGAAGAAAAATTTCTGCTGGAGGACGGGTGCCCTGTGAGCCGTGGCCACGGC
TATGAGCCCACGGACCCACGAGAAAGCCAGTG
mm4__chr13 113.11 Mbp_chr13_113109818 GGGGTCACTCTCCACCACCGCGTGGTGCCCACAGCTCACGGCGCTCCCGGGTGACGGTCCCCTCGGCTGTAGGGCGTCCT
GAAGAG
mm4__chr2 75.61 Mbp_chr2_75611861 CCTCTCCAGTGCCTCATGTCTCCCCTCTGCTCACAGCCTGCCCTGCCTCTGTAGCCTCTCTAGCATCCATTCCTCTCTGA
CTTAGCTCCTTCATCCTCCCTACTGCTGCTGGGACAGATCAA
mm4__chrX 78.10 Mbp_chrX_78104156 CCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGTGGCCAATAGCGGCTGCTCAGCAGGGCGCGCCGAGAGCAGCG
GCCGGGAAGGGGCGGTGCGGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTC
mm4__chr11 126.45 Mbp_chr11_126452827 AAGCCAGGTCTTTTGGGGAGGTGCCTCAGGACCCGACAGAGCCCGCAGCTCACATCTCGCCCAGCCGGTCTTTCTTCCCC
TCCTG
mm3__chr16 2.47 Mbp_chr16_2468137 CTTCTGACCGCCGCCTTCCTGTGCTGGCGCGCTCGGCGGAGGCGAAGGGCCGGGCGCAGGGAGCCGGGGGCGCCCTGAGC
CGCGGGCGAGGCCCCCTCCCATCGAGGTCACCGCGAG
mm4__chr2 240.58 Mbp_chr2_240575194 ACTTTCTCCGAGTGAGCAGCCCCCTGCCCGGGACGGCCCTGCTGCCATCTGCCCCCAGCCCCTCCCCGGCTCACAGGGCT
GCCTGCTCTTGCGGCAGTTGGGGTGGAACTG
mm3__chr21 42.84 Mbp_chr21_42837679 CAGCACCTTCTCCACCCGGGCCCCTCTGCTCACAGCGCTCCCCGCCCCCGTCTCCCCGAGGGGCGGGGAGCCAGGACATG
GCCCTGAAAGC
mm4__chr21 43.66 Mbp_chr21_43659492 CCTGAATTTTGGCAACCGAAAGGCAGCGCCGGCGCCACGTGCACACGGGCTGGGCCGCTCCGCCAGCTGCCAGGGCCACT
GCCGCGCTCACTCCCAGAGCGCGCTGCGAGCCGCGGCGCCTTTGTGACGCCATCAG
mm4__chr7 157.84 Mbp_chr7_157835614 AGGAAACAGGGATCTGCAGCAATGCCTCGGCTCTCAGGGCGCTCGGGAGGGTGCAGGGGCGAAATTAACAACATAGCCGA
AGGGTGCACTGCAAATGCGTGGGAAA
mm4__chr12 5.53 Mbp_chr12_5525463 AGGCAGAAACCACAGGACTGGCCCATATGGCCAGGCGGGCTCAGAGCCGTGGCCTGCTGTTGTTGGGGACAAG
mm4__chr6 168.37 Mbp_chr6_168370454 GCTTTGGACAGCATCACCACTGCCACCAGGGGGCACCCTCGGCTCACGCTGAGCCCGGCCCTCCTGCCCGCGGTCGGGCT
GGGACCGTCCTCTGGGCCTGCATCCGGCCTGGGACCCTTCTCCTTGTCTGTGCCC
mm4__chr17 81.29 Mbp_chr17_81288302 AACACCATTTCCTCTCCCGCCACCTCCTCACTCTGCCGCGGGAGCTGTGAGCCCGGGGGGCAGGATGGCCTTCTGCAGGG
AGGACCTAACA
mm4__chr4 3.46 Mbp_chr4_3461474 TCTGGGGAAACTTCTGGCTATAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNTCCTCCTCCTGCAGGAAGCTTCCTCCACCTTTCAGC
mm4__chr20 10.07 Mbp_chr20_10070346 TCTGAGCAATCCTCCCCTCTGCTGCCGGCCGCTCTGTCAGCCTAGGAAATATGGAGTAGGATGCAGAGTTGCCCATCAAT
GGACATGGTGGGTTATAAGCAACTGGGA
mm4__chr13 113.40 Mbp_chr13_113399997 CTGTTGTCACTTGCACCCACAGACCTGCGGCTCACAGTGGGCCTGTCGACAGCCGGAGGCACCGTAAAAAACTGCAGGGA
GGAGGCAGTCAACGAGTC
mm4__chr18 76.41 Mbp_chr18_76412967 CCTACCCGGAACCTACCAGAACCTAGCATCACTGCACGCCACCGCGGCCCACAGTGAGCCCTGCTCAGAGGCCCACTTCT
CCTTAGACATGAGACACTGCACCTGCCAAGGTAATGCTGAAACCAAGGCCTCTGTGAGG
mm4__chr2 598.29 Kbp_chr2_598288 CCCTCGGGTGATTCAGGACTAATCAGAGCATCAGGATGCTGGGCACGCCGTTAGCCGGGGCGATTCAGAGTCATCTGCTG
TGGAGTGGGGCTCAGAGTGAGGGATGTGGTCACTCACG
mm4__chr1 149.10 Mbp_chr1_149103259 CAACAAAGCTTGCGACAGCCGCAGCTCGACCCAGCTGTGTACCCGCGGGTCCCGGACTCACCGCCCGCCCGGCCTGGCGC
GGCGCCTTCACCTCGGAAACGCTGGGTGGACTTCGCTGTAA
mm4__chr1 145.43 Mbp_chr1_145425311 CAACAAAGCTTGCGACAGCCGCAGCTCGACCCAGCTGTGTACCCGCGGGTCCCGGACTCACCGCCCGCCCGGCCTGGCGC
GGCGCCTTCACCTCGGAAACGCTGGGTGGACTTCGCTGTAA
mm4__chr1 144.46 Mbp_chr1_144461369 AACAAAACTTGCGACAGCCGCAGCTCGACCCAGCTGTGTACCCGCGGGTCCCGGACTCACCGCCCGCCCGGCCTGGCGCG
GCGCCTTCACCTCGGAAACGCTGGGTGGACTTCGCTGTAA
mm4__chr17 63.70 Mbp_chr17_63700018 CCTCACCCCACACCCCTCGGCGCCTCTCCCGGTTCCGGAGCCGGACGCGGCCCCTCCCCCCGCGGCTCTCACCAGGCCCG
GCCTGGGCCGCGGGGCGGGATCGGTCTCCGGGGGCGCACGGGTACGAGGAGGG
mm4__chr19 23.12 Mbp_chr19_23117334 CAGTCCCCTTCAGCCATACGATGGCGGCCGCGCTGACAGCCGGGGCCCCGGCGTCCTGTCTCTTCCCTGCACTG
mm4__chr8 37.80 Mbp_chr8_37797353 GATGGGTTGATGGGCGCCGGGGGACGCAGGATGCGGGGGGCGCCCGCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAG
mm4__chr9 113.94 Mbp_chr9_113944065 CTGGATGGGGAACGTGATGGGTGATAGTTGAGGCTGGATTGCCTCTTACAACTATTAGGCTCCCTTTGCCCCTGCTCACA
CCTCGCCCTGCACGGTTTGATCCAATGTGGA
mm4__chr1 3.07 Mbp_chr1_3070724 GGTTTTCCGAAGCTCCGCTCCCTCGGCTCGGGGCCCGCCCGGCTCGTCCCAACAGCTGCCCCGCGGCCGTAGGCCCCGCG
CTCTGTGCACCAGGGAAAAGCAGCTCCCGAGTTTGT
mm4__chr10 120.59 Mbp_chr10_120589443 GTTAGTCTGCGAGTCCCAGCCTCACTGAAGAAAGAGGAGAGGCCCACAGCTGACAGCGCACCCAGCTTGCCTCTGGAGGG
GATCACCGAAGGCCCGGTATGACCAGTGTCCTGGGAGGATGGACACTAAGCCCTGG
mm4__chr6 170.30 Mbp_chr6_170295866 GGTCGTTTTGCGGTGGGGGGAGTCCGAACCGCGCCACGGGCCCGCGAGACGCCCACCTCTCGCGTGCGTGGAGCCCGGGG
CCGAGGCCAGGCCCGCTGCGAGCAGCGGAGGCATGTGCTGATTTGCATGAGAACGGGCG
mm4__chr5 159.05 Mbp_chr5_159051601 ACAACAACACAAACACCGCCGGCCCGGCTCGGTGCTCCGCTGCCCACAGTGCGCTCGGCACCAGGGCTGGATTTATAGCA
AGGAGCCCCGACTGTAAGAAGTAATTCATGAGTCCCCACAATGCC
mm4__chr11 19.71 Mbp_chr11_19712793 CCACACCCTCTTTGCAGTGAGACCCGCGGCCGCTGATTGGCGCACCGTGGACCAATGGGCGCGCCGGGGCGGCCCCGGCT
GTCAGCGCGCGCCGCAGCAGCGCCGGCAGCAGCCTGTCCTCCCC
mm4__chr16 4.39 Mbp_chr16_4392711 CCACTAGGCAGGAAGGAGGATTGCCAGAGAAGGAGCTCCCAGAAGGCCTCTCCCTCGAGCCGGCCCCTCGGCTCACACCG
GCCCTGGCTGCCAGCAGCGGTTTTCCTAATAGAAGCCAGGTGTGGGC
mm4__chr4 849.53 Kbp_chr4_849530 GCTTTCTCTTCATGTGCCTGGAACGCTGGGCGGGCGGTGACCCGGGGCTCGGAGCCCCACCCTGGCCACACCTGCTGTCG
CCCACGGGGTCCTCACGGTGGGGACCCAGGTCCCACGACGGCTCCCAACCTGTGGAGCTG
mm4__chr2 69.21 Mbp_chr2_69211893 GCTATTTCAGCCTACTCACCATATGTATTCCCACCTGTGGACCTCTGCTCACACCACCCCCGGCCCAAATTATGCTGTTC
CTTCTCTTCTCATTGTAAGTCAACCCACTCCTCTCGT
mm4__chr19 19.26 Mbp_chr19_19261517 CTGTGAGCAGCAGGACGCCCCAGGCCGCGGCCCAGAGCGCGCCGGGACCGAGGGCCGCCCGAGCGCACACCTGGGGGGCG
GGCACGGGGCGCTCAGTCCAGCCTCCCAGCCTTGGCTTTTCGGAGGCCT
mm3__chr2 238.24 Mbp_chr2_238239475 CGTCTGGCAGGGGTTACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGCAG
CACACCCTGGACCCGCCGCACCGTCAGCTCCAGCACTTCGGCGTTCTCCAGCTTG
mm3__chr10 131.97 Mbp_chr10_131971154 CCCAGAGGCACATGTCAGTGCCTCGGACCGTGGTCCAAGGGGAGTCCTGGGGCTGGGGGCCTTCCCCGGGCCGCGCCGCA
CTCCCGGCTCACAGCACGCTCGCCGCCTCCAGGGACAGATCACCT
mm4__chr1 175.19 Mbp_chr1_175192692 AAACTCACCTTTCGGTCCGCGGAAGGGCTTCCTGCAGGCCCAGGGAATCTCCTGGTTGCTGCCCCGTCTGACAGCGCCCC
GGGCCCTGGAGGGAGGTGTGGGTTCCTTTT
mm4__chr5 180.33 Mbp_chr5_180328579 AATTCCACGGTGAGCGATGTGCGGCTTGCCCAGGCCCACAGGGAGCCCGGCAGGGGTGCCAGCTGGGTAGACGGGGCAGT
GAGAGTCACAGGAAATGATAGCTGGGACCAGGCTTC
mm4__chr11 64.42 Mbp_chr11_64419552 CGGTCCAGACTCCTAGAGCTCAACAAATGGTCCTGTGAGAGCCTGGCGCTGTGTGAGCCCGGGAAGGCAGTGCAGTGCAG
TGGCCAGGTCATGTCACCCAGGCTAGG
mm4__chr8 144.48 Mbp_chr8_144478115 CCAGCGGACCCACTTGTGCGGCGGTCGGCGCGGGGCGCGAGGTGAGGCGCGGACTCCCGGGTCCTGCGGCGGGAGCGGAG
CGAGGGAGAG
mm4__chr12 68.81 Mbp_chr12_68808006 TAAAGGAGTGTCACAGCGCCAAACCTTGCTGGCTCCGCGCCAGGCCGAGCCCCACCCTCTCAGCTCGCGGCCAACACCCC
CACCCCGCCTCACAGCCCGCCGCGCCCGCGGGGCGACACCCCCCACTCCATCATCCCGGA
mm4__chr21 14.06 Mbp_chr21_14064609 GACAAGCAGCAAAGGTAGCGGGGGCTCAGCCCGGGGTGGGAGGGGGTCCCCAGGCGCGGCTTTCCGGCAGCCCCTGGGAC
GGGGGCCTTGGAGGTCGCCGGGCACCCTCTGAGCGGCGGAGCCAAACGGACCCTGAGCTG
mm4__chr21 13.98 Mbp_chr21_13979596 AAACAGCTGAGGGTCCGTTTGGCTCCGCCGCTCAGAGGGTGCCCGGCGACCTCCAAGGCCCCCGTCCCAGGGGCTGCCGG
AAAGCCGGACCTGGGGACCCCCTGCCACCCCGGGCTGAGCCCCCGCTACCTGTGCTGCTT
mm4__chr18 14.18 Mbp_chr18_14179679 AAGCAGCACAGGTAGCGAGGGCTCAGCCCGGGGTGGCAGGGGGTCCCCAGGTCCGGCTTTCTGGCAGCCCCTGGGACGGG
GGCCTTGGAGGTCGCCGGGCACCCTCTGAGCGGCGGAGCCAAACGGACCCTCAGCTGTTT
mm2__chr1 115.64 Mbp_chr1_115642028 TCTGCCTCTCCAGGAGCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNGGTGAGTCCCGCGAGAGG
mm4__chr7 100.90 Mbp_chr7_100895922 GAGCCGACTCTGACAGCCGCCGCCTCCGGCCCCCCGCACACACCCCCTCCGGGCCTCACAGCGCCCCCGCCCGCCCCCGC
CCACTGTCTCCGCCCCCGCCCCTCCCCGCTCTCCCGGGTGGCCACCTCCCCTCTCTCTCC
mm3__chr1 36.22 Mbp_chr1_36224421 TACATTTCCCAGGAGGCAGCGGGTCTACGCCGTCGCCGTCGTCGGAGAGCGGAGACGCTGGGCGCGCTGTGGGGCGGGGG
CGAGGTTCGGGCTGGTTGTTCCGTTGCGAGCTGCAGCTGCGATCTCTGTGGTAGG
mm4__chr5 140.65 Mbp_chr5_140647381 TTCAGCTCCACGTAGCGTTTCTCAATGAAGTCCCTGCGGGGCCGGAGAGAGCGCCGCGCGTGCTGTGGGCGGGGGCTTCC
CTCAACTTCAGGGAGGTCGAGGT
mm4__chr2 120.46 Mbp_chr2_120463678 GAGACGGAAAGGAAGTGCCAGGGTGCATCGTTGGTGAGGGCTTCCCGGCTCACAGGGAGCCTGGTCCTGCCATTGGCAGG
GCACTGACAGCTCCCTGGGAACGATGCGTCTATGG
mm4__chr19 1.01 Mbp_chr19_1007778 TCGCCATGCTCACGTGAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGCTGACCCCCGC
CCCCGGCCCCAGCAGGAGCGACCCCCCCAAGCTCAACGCCTTCATCATGGACA
mm4__chr22 37.52 Mbp_chr22_37519730 CGACACACCTTCCCCGGGCCACGCGCATCGCCTCCCCACGCCCACAGCGCGCCCGCCCGTGCGGGGCTAGCCGCTCACGC
CCGGCGCAGCCCGCGGGCACTCAAGCTGGGCT
mm4__chr11 79.44 Mbp_chr11_79439916 CCTCAGTTGGCTCCTAGACGAACATCAATACCGCGGCCGGGCGCCCGGTGAGGAGAGGCGGGCGCCCCGCGGGTTGCAGA
GGGCGAGGAGCTGGGGTCGCATCTCCAGGATCTGAGCTCCAACCACC
mm4__chr19 13.00 Mbp_chr19_12996534 GGCGGGGCTCCCAAATTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGCTGCCAGCGGTGGCCGCGCCTCTCCG
ACCCCGGACGTCGCAGCCTCTGCCCCCCCACCCCCAAACTTTCCTCGGCGCAAAC
mm4__chr10 44.39 Mbp_chr10_44385148 CGCTTTAGAGGGGAGAGCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNGTCTCTGACGCGCGACTC
mm4__chrX 141.18 Mbp_chrX_141177134 GAGCCCTCGCAGGAAGTGCGTGTCCACGGAGGCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNTACGCAAGCTCCGCCCACGAGGCCATTCGGACGGAC
mm4__chr9 136.81 Mbp_chr9_136808449 CTTGCCGGTTGTGGGGTGCGCTGGGCCCGCCGGGCGCTCCGGGAGCGGGGGCGCGNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNGCCGGGTCCTCGCGGCCCCCGAGCCGCGGGTCGTTTCCCAGCCGCACTC
mm4__chr9 135.70 Mbp_chr9_135702072 GATCGCCCTCCCTACCCCGCACCCCCGTCCCAGCCTGGGCCAGAGCCGCCGCGGGTCCCGGCGCGGCCGGCAGGGGGCGC
GCGCGGACAGACGCCTGCACCCGACCCTGGAACTGGCGTCTTTTCCT
mm4__chr8 19.76 Mbp_chr8_19757137 GGGCTGTGGGGATCTGCCGGCGCCACTGACGCCCCCGCGCGGCACACCGCGCCCGGCTGGCCCGGGAGGGGACCCGACTC
ACCCGAAG
mm4__chr8 10.73 Mbp_chr8_10730157 CGAAGAGGGCCCTCGTCCCGCGTGCCGGGTCTTCCCCAGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACTCACCCAGCATCTTGC
mm4__chr7 44.11 Mbp_chr7_44110090 AGATCCGAGCCTCCTCCATGCTGCGCCACGGCCTGGGGGCACAGCGCGGCCGGCTCAACATGCAGGTGGGCATTGGGATG
GGCCCATCTCCCAACTGGGATAAGGGACTCCTCCG
mm4__chr7 35.26 Mbp_chr7_35258403 TCCAGGCCTTGTAGCGTCTTCCCCGGGTCCCCGCGCGCCAGGCCCCGCAGCCTGCTCACAGGACAGCCCTCGGGGCTGCG
ACCCTCTCGCTCCTCCCCGCGGCCACTCGCTCCCCGTTCATC
mm4__chr7 29.99 Mbp_chr7_29989467 AGGCCACCTCTCCTCTGACCGCCCCCAGGCTCCCAGCCCGCCCGGGCCAGCACCGGGAATCGGCCTGGGGTAACGCTACC
CGCGGGTTCGGAGGGACAGCGGGAGGAGATGAAACGCAGGAGGTGG
mm4__chr7 149.15 Mbp_chr7_149147675 CCGCCTGTGGAGAGGACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTCCG
AGGCCCGGGCTAGACAGCGCAGGGCCAT
mm4__chr6 89.41 Mbp_chr6_89412166 TCTAGAGCTCAGCGGGGCCCGGCGGAAGCGGGGGCCGCGCGTCCCCCGGCGGGCGGCGCCCAGGTCCGGGTCCCGCGGTT
CCCAGCGCGCCCGAAGCCCCCTCCCCCGCCCCGCCCGCCGGCGGAGGAGTCAGCCGGAG
mm4__chr4 56.05 Mbp_chr4_56049088 GGAGCATGTGACCACGCCGCGCGGGGCCGGGCGCGCAGTGCCGCGCGGTGGCGGCAGTGGGGAGAGCAATGGAG
mm4__chr4 4.86 Mbp_chr4_4860195 CCAAGGAGAGCGCCCTGGCGCCCTCCGAGGGCGTGCAGGCGGCGGGTGGCTCGGCGCAGCCACTGGGCGTCCCGCCGGGG
TCGCTGGGAGCCCCGGACGCGCCCTCTTCGCCGCGGCCGCTCGGCCATTTCTCGGTGG
mm4__chr3 128.49 Mbp_chr3_128489251 CCCGATTCGCCAGGAAATGAACTTTTTAATAATCCGAGGAGGGAGGAAAGCCCTCGGTCCCCTCGGCTCCGGGGGCGCCC
GGCTGGGCCCAGCTCCGCGGGCGCGCCCCCGTCACCCCCTCCCAGGCCAGTTCAG
mm4__chr3 126.08 Mbp_chr3_126084284 GACCTCGGACGACTTGGCGCTCCGGGACAGCAGCAGACGCCCAAGCTCCGGCAGCGCAGAGCCCCGCCGGGGGCCCGGGG
GGCTGGGAGCCGGGGATCGCGGGGACGGAAGTGGGGGACCGAGTCTCGCGAG
mm4__chr3 101.85 Mbp_chr3_101849797 CCTGAGCTACTTCTACGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCTGCTCGGTCTTGGGCCCCT
CGGCGCCCGGCTCGCCCGGCTCCGACTCCTCCGACTTCTCCTCTGCCTCGTC
mm4__chr22 50.19 Mbp_chr22_50194265 CAGTTCTCAGAACCCAGGAGGGTTCTAGAGCCCAGGCTGCCAGCGGGCCCGGCGGCGTCCTTGGGGTGGGCCCGGCACCA
CAACGGCCTGTGCTGCTGTTGCAGCCGACGTGGACGCCTTCAACCTGCTCCTGGAG
mm4__chr22 45.95 Mbp_chr22_45952558 AACCGAGCTGGGGAAATGAGATGGATGGGAACCCTGCCGGGCACCCTGTTTGCCGTGGGGCAGAGCCCGCCTCAGTCCCA
GCCTGAGGGACACTCTCCAGTCCCAAATTCTCCCAGCAGGCTCAG
mm4__chr21 36.71 Mbp_chr21_36708069 CAGGGGTCGGGAGCTAGGCTCGTGGGCCGGGAGACGCCGGGCGCGTTGTCCTCCGGGGAGGTTGGGGTGCAGGCGGTGCA
CCGACCCTCGCCATCTGGCGCTGCAGCCACCAGCCACGGCGCTTAGTGGAGGGTCTG
mm4__chr19 48.50 Mbp_chr19_48499739 GGAACGGTGAGGAGAGGGTCCCCGGGCGGGGCGCACTGTGTGCAGGGGGCCAGGGCCAGGGGAAGGGACCGTCTCGGGGT
GGGGGTGGCGGCGGTGGGGGCCGGGGAGGCCGCTCGGAGAGCAAGTAGGTGAGCTGCA
mm4__chr19 2.34 Mbp_chr19_2340129 GGTCATCGGGATCTTCTGCCTGGCCTCCGCCACCGGCCTCTACAGCTGCCTGGCGCCCTGTGTGCGGCGGCTGCCCTTCG
GCAAGTGCAGGTGAGTCTGCCCTGCTGGCCCCGACGTGCCTGACTCTGTGCGGTGATG
mm4__chr19 10.55 Mbp_chr19_10552967 CTCAGGACCACAGCCTGGACGACCTCTGCTCCCAGCTCGCCCAGCCCACACTCCGGCTCCCTCGCACAGGGCGGCTCCTC
AGGGCCAAACGCCCCAGCTCTGAGGACTTTGTGTTTTTATAAAGGGAGGGGATGAGGGG
mm4__chr18 3.01 Mbp_chr18_3012809 GGGTTCAGAGTCCTCAGCAGCCACCACTGACGCCCCGACGCCCCCGGCTCCCCGCGCACCCCGCCGGTCCGTCCTGCGGC
GGCGGCGGTAGGGGCCGCGTCTAC
mm4__chr17 82.09 Mbp_chr17_82090374 AAGGCAGCCTCCTTTCTTGGAGGTGCTGGGGGCCCCTCCGGGAGACCTACCCACGGCTGCCATGGCGCCCGGCGGGAGAT
GGGCTTCTTTGATGCACTGTCCCCTCC
mm4__chr17 79.86 Mbp_chr17_79862053 CTGAGTCGATGTGATGGCGTGGCGAGTGGCCACAGACTCTGGGAGCCAGCCGGGGAGCACAGCGTGCCCGGCAGAAGGAG
GTGATGCTGGCTGAGCAGAAAGGTCA
mm4__chr17 72.47 Mbp_chr17_72465826 TCCCTGCGCTTGTTCCTATGGCTGCCCCTCAACCCAGCTCACCGCTGCTCCCAGCTCGCTCGGCACTCTGCAAGGATTGG
CATTTGCTTCGGGCAGACTCTTCTCTGTCCCTGCCT
mm4__chr17 15.94 Mbp_chr17_15944674 GAGACAGCTCCGGTGGTGCTGCGCTGCCCGAGACTGCTGCACGAGCGGGGACTGCGGCCTCGGCTCCCTGCCTGCCCGGC
AGCCCCCCATCCAGTCGGCCTGCCCGGGGTGCGCAGGGTTAGCCTGGAGTGAGCTC
mm4__chr17 1.52 Mbp_chr17_1516872 CAACACTGCCCCAGACGACGCGAGGTGTGCGGCCGCCACTAGACCCGGCCCAGCCCGCGCCCGCCGTCCGCAGCTCCCAG
CGTGCCCCGCGCCAGGGGCCGTCTTCCGGCGGAAGTTGTCTCTT
mm4__chr16 88.79 Mbp_chr16_88785438 CTCCCCTCCCGCAGTCTTGGCCCCGCGGCTCCGAGCGTGCGCGGCGGTGGGCAGGGGCGCTTCTCGCTGGGTGGGCTGCG
GCTGGGGCCCGGGCGCTACATAAGGGGTC
mm4__chr16 22.81 Mbp_chr16_22814857 CGGCCAGAAACTTCTCCAGAAGTCCCGCCCCTGTGATCCCTCCGGGCCGACGCCCAGCGAGCCCAGCGCTCCCAGCGCGC
CCGCCGCCGCCGTGCCCGCCCCTCGCCTCTCCGGTTCCAACCACTCCGGCTCACCCAAG
mm4__chr15 79.09 Mbp_chr15_79091679 CACGGCATTTATCCGAGAACGTCACAGCGCCCGCATGCTGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNATCGCTGTCTGCCGCAGAGGGCAGCGAGCTCG
mm4__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135991 AGGAATGGTTGTGCCTGCTGCAGAAACTCCGGGCTCGCCAGGAGCTCATCCAGCAGCTGGCCGGAGGGGAGTGCAGAAGA
GCACCCCGGCTCCCAGAGCGCCTGGGAGAGCGCCGGGATGCCTTGCATCTGCCCCTGCT
mm4__chr13 23.95 Mbp_chr13_23945645 GGGATGGTAAGCAGCTGAGGCTTGATTTGGCTCCGCGGCTCCAAGGGTGCCCGGCCCCATCCAAGGCCCCGTCCCATGGG
TTGCGGGGAAGCCGGGCCTGGGGACCCCCCTCTCACCCTGGGCTGA
mm4__chr12 120.10 Mbp_chr12_120101002 AGAGATGCCAAGAAACGCCTGCAGCCATGGCCCCGGCGCACAGCCCCCGCGGCTCCCTGGGCGCCTCTGCCTCATCCGGG
GCCCAGGAAAGGGGCTTTATCTGAGAGCAGAGAATTTGTCTGGGGATGAAGTCAGGG
mm4__chr11 44.07 Mbp_chr11_44066188 ACCGCCCTCAGTCTCCTAGTCCGGGCGCGCTGAGCGCAGGGGGGCGCTGTCCTCACCCACGCGTAAGTCGCCGCAGGGCG
TTTCCGGGCGGAGAAAACCTACACGT
mm4__chr11 119.47 Mbp_chr11_119470752 CGGCCCATTTGTAGAGCTCAGAGACCCCGGCTCCCACCCCGCCTGGCCCCTCTCCCTCCAGACTCTCTGATGCCTGC
mm4__chr11 1.34 Mbp_chr11_1336928 CCTCAGGTGTCTCGAGATGGCCCGGGCTCCGAGCGCTCCCGGCGCTTACGCTAGAGCCTCCTCCTG
mm4__chr10 988.31 Kbp_chr10_988313 CTCTCCTCTGTACCCTCGCTCCTGAGGCCCTCACACGCCCTCCGTGGCTGACCTCCAAGCCGGGCGGGTGCGCTGTGCGA
CGCGGAAAGCGCCGTAGAGCACGCAGAACACGATTG
mm4__chr10 133.47 Mbp_chr10_133467592 CATGAGGGCAGAGTCAGTGGCTGGAGTCCCCATCGGAGAGGCCTGCCCTGTGGTGGGAGAAGAGGGCTGGTCGGGTGTGC
TGGGAGCCGCGGGTCTGGCAGGGGCATATGCTGCAGCTCACTCAGC
mm4__chr1 84.51 Mbp_chr1_84506775 GGCATTTTCAGTTCCACCCCCAACCCCCAGCCCCGCTGACCCGAGGCGGAGAGCAGGCGCGGAGGTCGCTGTCGCGTACC
CAGCGGAGCCGGGCGGGCGGGGAGCAGGGGCGCTGATTCGCTGGAGAAGGG
mm4__chr1 210.33 Mbp_chr1_210329288 GAAGAAGACAAAAGCGGCGGGGGCCGCGCGCGCAGTGCGCCCGGGCAAAGGCGGGGATCTAGGCGCCGGGACAAGTCCGC
GCACGGCCCTGCCCACGTCCTCTCTCCTTGGCTTC
mm3__chr8 101.21 Mbp_chr8_101206184 GCTGAGTCACATTCCCGGAGCCCCGCCACCCAGGACACCGCGGCCCCGGCCCCCAGCGAGCCCGGCGGACCCAGCGGCTC
TGAACGCGGACGCACAGCCTTTGTTCTTTCACAGCCGCC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.