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The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| Bae-Score | Sequence | Effect |
|---|---|---|
| CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | (Wild-type sequence) | |
| 311.6 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-----GCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 5 bp deleted → frameshift |
| 298.2 | CGCGGCGATATCATCATCCATGGTGAGCTGCG--------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 14 bp deleted → frameshift |
| 282.0 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-------GCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 7 bp deleted → frameshift |
| 269.4 | CGCGGCGATATCATCATCCATGGTGAGCTGCG----------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 16 bp deleted → frameshift |
| 255.2 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC---------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 9 bp deleted → no frameshift |
| 247.0 | CGCGGCGATATCATCATCCATGGTGAGC----------------------------CGAGGTCGCCCCCGCCCTGGCCAC | 28 bp deleted → frameshift |
| 245.0 | CGCGGCGATATCATCATCCATGGTGAGCTG---------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 21 bp deleted → no frameshift |
| 242.8 | CGCGGCGATATCATCATCCATGGTGAGCTGCG----------GGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 10 bp deleted → frameshift |
| 224.5 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC----------------CGAGGTCGCCCCCGCCCTGGCCAC | 16 bp deleted → frameshift |
| 191.1 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAG--------------------------GTCGCCCCCGCCCTGGCCAC | 26 bp deleted → frameshift |
| 175.0 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAG---------------------CCGAGGTCGCCCCCGCCCTGGCCAC | 21 bp deleted → no frameshift |
| 170.8 | CGCGGCGATATCATCATCCATGGTGAGC-----------------GCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 17 bp deleted → frameshift |
| 162.8 | CGCGGCGATATCATCATCCATGGTGAGCTGC------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 18 bp deleted → no frameshift |
| 154.8 | CGCGGCGATATCATCATCCATGGTGAGC-------------------GCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 19 bp deleted → frameshift |
| 147.2 | CGCGGCGATATCATCATCCATGG--------------------GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 20 bp deleted → frameshift |
| 140.0 | CGCGGCGATATCATCATCCATGG---------------------CGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 21 bp deleted → no frameshift |
| 116.1 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGA-------------------GCCGAGGTCGCCCCCGCCCTGGCCAC | 19 bp deleted → frameshift |
| 114.8 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-------------------------CCCCGCCCTGGCCAC | 25 bp deleted → frameshift |
| 114.8 | CGCGGCGATATCATCATCCATGGTGAGCTGC-------------------------CGAGGTCGCCCCCGCCCTGGCCAC | 25 bp deleted → frameshift |
| 113.6 | CGCGGCG---------------------------------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 39 bp deleted → no frameshift |
| 105.0 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAG---------------------GTCGCCCCCGCCCTGGCCAC | 21 bp deleted → no frameshift |
| 96.0 | CGCGGCGATATCATCATCCATGGTGAG---------------------------------GTCGCCCCCGCCCTGGCCAC | 33 bp deleted → no frameshift |
| 95.1 | CGCGGCGATATCATCATCCATGGTGAGCTG-----------------------AGCCGAGGTCGCCCCCGCCCTGGCCAC | 23 bp deleted → frameshift |
| 90.3 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGA------------------------GGTCGCCCCCGCCCTGGCCAC | 24 bp deleted → no frameshift |
| 90.0 | CGCGG--------------------------------------GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 38 bp deleted → frameshift |
| 88.8 | CGCG--------------------------------------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 44 bp deleted → frameshift |
| 84.8 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-------------------------------CCTGGCCAC | 31 bp deleted → frameshift |
| 81.9 | CGCGGCGATATCATCATCCATGGTG--------------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 26 bp deleted → frameshift |
| 80.8 | CGCGGCGATATCATCATCCATGGTGAGCTGCG--------------------------------CCCCCGCCCTGGCCAC | 32 bp deleted → frameshift |
| 77.4 | CGCGGCG-----------------------------------------CTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 41 bp deleted → frameshift |
| 75.0 | CGCGGCGATATCATCATCCATGGT--------------------------------------CGCCCCCGCCCTGGCCAC | 38 bp deleted → frameshift |
| 73.2 | CGCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------CCCCGCCCTGGCCAC | 34 bp deleted → frameshift |
| 73.2 | CGCG------------------------------------------CGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 42 bp deleted → no frameshift |
| 70.5 | CGCGGCGATATCATCATCCATG-----------------------------TGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 29 bp deleted → frameshift |
| 69.6 | CGCGGCG-----------------------------------GGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 35 bp deleted → frameshift |
| 63.6 | CGCGGCGATATCATCATCCATG-------------------------------AGCCGAGGTCGCCCCCGCCCTGGCCAC | 31 bp deleted → frameshift |
| 62.8 | CGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC-------------------------------------CAC | 37 bp deleted → frameshift |
| 62.8 | CGCGGCGATATCATCATCCATGGTGAGC-------------------------------------CCCCGCCCTGGCCAC | 37 bp deleted → frameshift |
| 60.0 | CGCGGCGATATCATCATCCATGGTGAGCTGCG--------------------------------------CCCTGGCCAC | 38 bp deleted → frameshift |
| 60.0 | CGCGGCGATATCATCATCC--------------------------------------GAGGTCGCCCCCGCCCTGGCCAC | 38 bp deleted → frameshift |
| 60.0 | CGC----------------------------------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 46 bp deleted → frameshift |
| 54.0 | CGCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------------CCTGGCCAC | 40 bp deleted → frameshift |
| 54.0 | CG----------------------------------------GGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 40 bp deleted → frameshift |
| 52.5 | CGCGGCGATATCATCATCCATGGTGAGCTG---------------------------------------------GCCAC | 45 bp deleted → no frameshift |
| 46.4 | CGCGGCGATATCATCATCCATGGTGAGC-------------------------------------------CCTGGCCAC | 43 bp deleted → frameshift |
| 46.4 | CGCGGC-------------------------------------------TGTGAGCCGAGGTCGCCCCCGCCCTGGCCAC | 43 bp deleted → frameshift |
| 40.0 | CGCGGCGATATCATCATCCATGGTGAGCTGC----------------------------------------------CAC | 46 bp deleted → frameshift |
| 39.0 | CGCGGCGA---------------------------------------------------GGTCGCCCCCGCCCTGGCCAC | 51 bp deleted → no frameshift |
| 38.0 | CGCGGCGATATCATCATCC-----------------------------------------------CCCGCCCTGGCCAC | 47 bp deleted → frameshift |
| 36.4 | CGCGGCGATATCATCATCC------------------------------------------------CCGCCCTGGCCAC | 48 bp deleted → no frameshift |
| 35.5 | CGCGGCGATATCATCATCCATGG-----------------------------------------------------CCAC | 53 bp deleted → frameshift |
| 34.4 | CGCGGCGATATCATCATCCATGGTGAGC-------------------------------------------------CAC | 49 bp deleted → frameshift |
| 34.4 | CGCGGCGATATCATCATCC-------------------------------------------------CGCCCTGGCCAC | 49 bp deleted → frameshift |
| 32.8 | CGCGGCGATATCATCATCC--------------------------------------------------GCCCTGGCCAC | 50 bp deleted → frameshift |
| 32.8 | CGCGGC--------------------------------------------------CGAGGTCGCCCCCGCCCTGGCCAC | 50 bp deleted → frameshift |
| 31.5 | CGCGGCGATATCATCATC---------------------------------------------GCCCCCGCCCTGGCCAC | 45 bp deleted → no frameshift |
| 30.0 | CGCGGCGA----------------------------------------------GCCGAGGTCGCCCCCGCCCTGGCCAC | 46 bp deleted → frameshift |
| 28.4 | CGCGGCGATATCATCATCC-----------------------------------------------------CTGGCCAC | 53 bp deleted → frameshift |
| 28.4 | CGC-----------------------------------------------------CGAGGTCGCCCCCGCCCTGGCCAC | 53 bp deleted → frameshift |
| 27.3 | CGCGGCGATATCATC------------------------------------------------GCCCCCGCCCTGGCCAC | 48 bp deleted → no frameshift |
| 27.0 | CGC--------------------------------------------------------------CCCCGCCCTGGCCAC | 62 bp deleted → frameshift |
| 26.8 | CGCGGCGATATCATCATCC------------------------------------------------------TGGCCAC | 54 bp deleted → no frameshift |
| 26.8 | CGCG------------------------------------------------------AGGTCGCCCCCGCCCTGGCCAC | 54 bp deleted → no frameshift |
| 26.0 | CGCGGCGATATCATCATCCA-----------------------------------------------------------C | 59 bp deleted → frameshift |
| 24.6 | CGCGGCGATATCATCATCCATGGTG--------------------------------------------------GCCAC | 50 bp deleted → frameshift |
| 24.4 | CGCGG--------------------------------------------------------TCGCCCCCGCCCTGGCCAC | 56 bp deleted → frameshift |
| 24.4 | CG--------------------------------------------------------AGGTCGCCCCCGCCCTGGCCAC | 56 bp deleted → frameshift |
| 23.4 | CGCGGCGATATC---------------------------------------------------GCCCCCGCCCTGGCCAC | 51 bp deleted → no frameshift |
| 23.2 | CGCGGCG---------------------------------------------------------CCCCCGCCCTGGCCAC | 57 bp deleted → no frameshift |
| 20.8 | CGCGGC-----------------------------------------------------------CCCCGCCCTGGCCAC | 59 bp deleted → frameshift |
| 20.0 | CGCG------------------------------------------------------------CCCCCGCCCTGGCCAC | 60 bp deleted → no frameshift |
| 19.8 | CGC--------------------------------------------------------------------CCTGGCCAC | 68 bp deleted → frameshift |
| 17.4 | CGCGGC-----------------------------------------------------------------------CAC | 71 bp deleted → frameshift |
| 17.2 | CGCGGCG---------------------------------------------------------------CCCTGGCCAC | 63 bp deleted → no frameshift |
| 15.6 | CGCGGC-----------------------------------------------------------------CCTGGCCAC | 65 bp deleted → frameshift |
| 14.8 | CGCG------------------------------------------------------------------CCCTGGCCAC | 66 bp deleted → no frameshift |
| 12.9 | CGCGGCGATATCATCA---------------------------------------------------------------C | 63 bp deleted → no frameshift |
| 11.1 | CGCGGCGATATCA------------------------------------------------------------------C | 66 bp deleted → no frameshift |
| 10.0 | CGC--------------------------------------------------------------------------CAC | 74 bp deleted → frameshift |