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Contents:

PCR primers for off-targets of GCCGGAAGTGGCCAGGGCGG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529700_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529700_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm3__chr12 57.09 Mbp_chr12_57085329_F Primer3: not found at this Tm N.d. 0.64
mm3__chr12 57.09 Mbp_chr12_57085329_R Primer3: not found at this Tm N.d. 0.64
mm4__chr22 38.62 Mbp_chr22_38615975_F Primer3: not found at this Tm N.d. 0.62
mm4__chr22 38.62 Mbp_chr22_38615975_R Primer3: not found at this Tm N.d. 0.62
mm2__chr20 63.89 Mbp_chr20_63890192_F TCGTCGGCAGCGTCCTGTGCTGTCCTTCCTAGGG 59.4 0.61
mm2__chr20 63.89 Mbp_chr20_63890192_R GTCTCGTGGGCTCGGTCCTTCAACATTCTGATGGGCT 59.6 0.61
mm4__chr15 68.58 Mbp_chr15_68580604_F TCGTCGGCAGCGTCGGGACTTGGGAGCATTGTGA 59.9 0.56
mm4__chr15 68.58 Mbp_chr15_68580604_R GTCTCGTGGGCTCGGAAGCATCTTACCCCAGGTGG 59.3 0.56
mm3__chr12 132.75 Mbp_chr12_132745993_F TCGTCGGCAGCGTCCTGTGCTGTCCTTCCTAGGG 59.4 0.55
mm3__chr12 132.75 Mbp_chr12_132745993_R GTCTCGTGGGCTCGGTTCTTAAAGGGAGCCTGGGC 59.6 0.55
mm4__chr3 99.82 Mbp_chr3_99818098_F Primer3: not found at this Tm N.d. 0.55
mm4__chr3 99.82 Mbp_chr3_99818098_R Primer3: not found at this Tm N.d. 0.55
mm4__chr19 58.02 Mbp_chr19_58020285_F TCGTCGGCAGCGTCGATGGGAACTGGGTCCTGTC 59.7 0.53
mm4__chr19 58.02 Mbp_chr19_58020285_R GTCTCGTGGGCTCGGCCTGGCTTTCTTTTGAACCTGG 59.9 0.53
mm4__chr17 77.36 Mbp_chr17_77364602_F TCGTCGGCAGCGTCAGAGCGGAGAGGTTCCTCTT 59.9 0.53
mm4__chr17 77.36 Mbp_chr17_77364602_R GTCTCGTGGGCTCGGTAGAACTGTGGGCTGGCAAG 59.9 0.53
mm4__chr14 42.73 Mbp_chr14_42734145_F Primer3: not found at this Tm N.d. 0.50
mm4__chr14 42.73 Mbp_chr14_42734145_R Primer3: not found at this Tm N.d. 0.50
mm4__chr11 60.94 Mbp_chr11_60938450_F Primer3: not found at this Tm N.d. 0.50
mm4__chr11 60.94 Mbp_chr11_60938450_R Primer3: not found at this Tm N.d. 0.50
mm4__chr8 10.33 Mbp_chr8_10334856_F TCGTCGGCAGCGTCTGTGCCTTACTCCCTCTGGT 60.1 0.48
mm4__chr8 10.33 Mbp_chr8_10334856_R GTCTCGTGGGCTCGGTAGCAACGGGAAGATGGCTC 59.8 0.48
mm3__chr14 75.47 Mbp_chr14_75470354_F TCGTCGGCAGCGTCAGTTACGGCCGTTGTGTGTA 59.6 0.45
mm3__chr14 75.47 Mbp_chr14_75470354_R GTCTCGTGGGCTCGGAAGGATGGAAATGCACCCCG 60.6 0.45
mm4__chr16 10.84 Mbp_chr16_10835454_F TCGTCGGCAGCGTCATAACAGACCAGCCAGCCAC 60.0 0.44
mm4__chr16 10.84 Mbp_chr16_10835454_R GTCTCGTGGGCTCGGCAGGATTCATGGCCCTCTCC 59.8 0.44
mm4__chr3 98.52 Mbp_chr3_98522622_F TCGTCGGCAGCGTCCCACCTCCTGGGCCTCAC 61.7 0.43
mm4__chr3 98.52 Mbp_chr3_98522622_R GTCTCGTGGGCTCGGGATTTGTCCCAGGGCCGAG 60.4 0.43
mm4__chr8 144.26 Mbp_chr8_144257730_F Primer3: not found at this Tm N.d. 0.43
mm4__chr8 144.26 Mbp_chr8_144257730_R Primer3: not found at this Tm N.d. 0.43
mm4__chr22 43.23 Mbp_chr22_43226331_F Primer3: not found at this Tm N.d. 0.43
mm4__chr22 43.23 Mbp_chr22_43226331_R Primer3: not found at this Tm N.d. 0.43
mm4__chr17 4.90 Mbp_chr17_4895619_F Primer3: not found at this Tm N.d. 0.42
mm4__chr17 4.90 Mbp_chr17_4895619_R Primer3: not found at this Tm N.d. 0.42
mm4__chr3 129.02 Mbp_chr3_129022442_F TCGTCGGCAGCGTCACATGAAGATCTGGAGCCAGG 59.5 0.42
mm4__chr3 129.02 Mbp_chr3_129022442_R GTCTCGTGGGCTCGGGGCCAGATGTTGCTCTTCCT 60.0 0.42
mm4__chr11 34.71 Mbp_chr11_34709602_F Primer3: not found at this Tm N.d. 0.41
mm4__chr11 34.71 Mbp_chr11_34709602_R Primer3: not found at this Tm N.d. 0.41
mm4__chr5 170.18 Mbp_chr5_170183106_F TCGTCGGCAGCGTCCACTCTCAGCCCCTTTCCTG 60.0 0.41
mm4__chr5 170.18 Mbp_chr5_170183106_R GTCTCGTGGGCTCGGTGGTTAGACAGAAGAGCTAAGCC 59.8 0.41
mm3__chr14 87.34 Mbp_chr14_87336311_F Primer3: not found at this Tm N.d. 0.40
mm3__chr14 87.34 Mbp_chr14_87336311_R Primer3: not found at this Tm N.d. 0.40
mm4__chr15 77.03 Mbp_chr15_77034245_F TCGTCGGCAGCGTCCAGCACAATGGCTCAGCAAG 60.1 0.39
mm4__chr15 77.03 Mbp_chr15_77034245_R GTCTCGTGGGCTCGGAGCAAAGGACAGGGTGCAAG 60.8 0.39
mm4__chr22 43.22 Mbp_chr22_43223240_F TCGTCGGCAGCGTCGCGAGAAAGACACTTGCCTG 59.4 0.39
mm4__chr22 43.22 Mbp_chr22_43223240_R GTCTCGTGGGCTCGGTCCCCATTAGGCCATACCCA 60.0 0.39
mm4__chr6 40.89 Mbp_chr6_40891281_F TCGTCGGCAGCGTCCCAGGAGGAACAAGAGCAGG 60.0 0.39
mm4__chr6 40.89 Mbp_chr6_40891281_R GTCTCGTGGGCTCGGGCATGAAGCACATCTCAGTG 57.1 0.39
mm4__chr16 71.41 Mbp_chr16_71410048_F Primer3: not found at this Tm N.d. 0.36
mm4__chr16 71.41 Mbp_chr16_71410048_R Primer3: not found at this Tm N.d. 0.36
mm3__chr2 239.48 Mbp_chr2_239483083_F Primer3: not found at this Tm N.d. 0.35
mm3__chr2 239.48 Mbp_chr2_239483083_R Primer3: not found at this Tm N.d. 0.35
mm4__chr2 109.46 Mbp_chr2_109456169_F Primer3: not found at this Tm N.d. 0.35
mm4__chr2 109.46 Mbp_chr2_109456169_R Primer3: not found at this Tm N.d. 0.35
mm4__chrX 143.63 Mbp_chrX_143634511_F TCGTCGGCAGCGTCCCACCCGAGACCTGTACAAC 60.0 0.34
mm4__chrX 143.63 Mbp_chrX_143634511_R GTCTCGTGGGCTCGGAAAGGATCCGGGTGTCCTTG 59.6 0.34
mm4__chr16 15.12 Mbp_chr16_15122735_F Primer3: not found at this Tm N.d. 0.33
mm4__chr16 15.12 Mbp_chr16_15122735_R Primer3: not found at this Tm N.d. 0.33
mm4__chr16 14.94 Mbp_chr16_14938171_F TCGTCGGCAGCGTCCCTGCCCTAAGCCACCTC 59.4 0.33
mm4__chr16 14.94 Mbp_chr16_14938171_R GTCTCGTGGGCTCGGCGCATGTCCTGGTCCTTTCA 60.3 0.33
mm4__chr7 97.91 Mbp_chr7_97907438_F Primer3: not found at this Tm N.d. 0.33
mm4__chr7 97.91 Mbp_chr7_97907438_R Primer3: not found at this Tm N.d. 0.33
mm3__chr8 5.73 Mbp_chr8_5734064_F TCGTCGGCAGCGTCTGGAACCAGCCGAAGATTCC 60.0 0.32
mm3__chr8 5.73 Mbp_chr8_5734064_R GTCTCGTGGGCTCGGCTACCCACCATACTGCAGGC 60.1 0.32
mm3__chr1 103.96 Mbp_chr1_103961103_F Primer3: not found at this Tm N.d. 0.32
mm3__chr1 103.96 Mbp_chr1_103961103_R Primer3: not found at this Tm N.d. 0.32
mm4__chr17 69.21 Mbp_chr17_69213766_F Primer3: not found at this Tm N.d. 0.32
mm4__chr17 69.21 Mbp_chr17_69213766_R Primer3: not found at this Tm N.d. 0.32
mm4__chr2 1.50 Mbp_chr2_1503596_F TCGTCGGCAGCGTCTCCTTGCTCTCAGAGGACCT 59.5 0.32
mm4__chr2 1.50 Mbp_chr2_1503596_R GTCTCGTGGGCTCGGGGAATTTGGCCAAGTGCAGG 60.0 0.32
mm4__chr8 8.09 Mbp_chr8_8087600_F Primer3: not found at this Tm N.d. 0.32
mm4__chr8 8.09 Mbp_chr8_8087600_R Primer3: not found at this Tm N.d. 0.32
mm4__chr8 7.20 Mbp_chr8_7199905_F Primer3: not found at this Tm N.d. 0.32
mm4__chr8 7.20 Mbp_chr8_7199905_R Primer3: not found at this Tm N.d. 0.32
mm4__chr8 12.64 Mbp_chr8_12637569_F Primer3: not found at this Tm N.d. 0.32
mm4__chr8 12.64 Mbp_chr8_12637569_R Primer3: not found at this Tm N.d. 0.32
mm3__chr15 42.14 Mbp_chr15_42142746_F Primer3: not found at this Tm N.d. 0.31
mm3__chr15 42.14 Mbp_chr15_42142746_R Primer3: not found at this Tm N.d. 0.31
mm4__chr4 1.10 Mbp_chr4_1097012_F TCGTCGGCAGCGTCACAGCCTCGATCAACAGGAC 59.7 0.31
mm4__chr4 1.10 Mbp_chr4_1097012_R GTCTCGTGGGCTCGGTTTTGAGGAGGGAGCGTGTC 59.9 0.31
mm3__chr8 144.42 Mbp_chr8_144416551_F TCGTCGGCAGCGTCGGTACTGCCACTAGCCTCCT 60.6 0.31
mm3__chr8 144.42 Mbp_chr8_144416551_R GTCTCGTGGGCTCGGTGGAAAGGTAACAGCCCCAC 59.8 0.31
mm4__chr2 45.54 Mbp_chr2_45542300_F Primer3: not found at this Tm N.d. 0.31
mm4__chr2 45.54 Mbp_chr2_45542300_R Primer3: not found at this Tm N.d. 0.31
mm3__chr3 52.37 Mbp_chr3_52373357_F Primer3: not found at this Tm N.d. 0.30
mm3__chr3 52.37 Mbp_chr3_52373357_R Primer3: not found at this Tm N.d. 0.30
mm4__chr5 178.72 Mbp_chr5_178718368_F TCGTCGGCAGCGTCCCCAGTCCTCAGCTACCTGT 60.6 0.30
mm4__chr5 178.72 Mbp_chr5_178718368_R GTCTCGTGGGCTCGGTGTAGTGAGAGTCCAGGTGGA 59.5 0.30
mm3__chr15 28.89 Mbp_chr15_28885993_F Primer3: not found at this Tm N.d. 0.30
mm3__chr15 28.89 Mbp_chr15_28885993_R Primer3: not found at this Tm N.d. 0.30
mm4__chr1 19.37 Mbp_chr1_19370136_F TCGTCGGCAGCGTCCCAAGGCTCCCAGAATGACA 59.6 0.29
mm4__chr1 19.37 Mbp_chr1_19370136_R GTCTCGTGGGCTCGGGGATTCCCAGTGGTCGTGG 60.0 0.29
mm4__chr2 44.95 Mbp_chr2_44949885_F TCGTCGGCAGCGTCCTGGAGCTGAGATGCCCG 59.8 0.28
mm4__chr2 44.95 Mbp_chr2_44949885_R GTCTCGTGGGCTCGGCTGGGGCCAGGGAGCTTC 62.1 0.28
mm4__chr4 8.82 Mbp_chr4_8821159_F Primer3: not found at this Tm N.d. 0.28
mm4__chr4 8.82 Mbp_chr4_8821159_R Primer3: not found at this Tm N.d. 0.28
mm4__chr21 29.44 Mbp_chr21_29436420_F Primer3: not found at this Tm N.d. 0.28
mm4__chr21 29.44 Mbp_chr21_29436420_R Primer3: not found at this Tm N.d. 0.28
mm4__chr7 44.23 Mbp_chr7_44226541_F TCGTCGGCAGCGTCGGCCACTCAAGGTGCTACTT 59.9 0.28
mm4__chr7 44.23 Mbp_chr7_44226541_R GTCTCGTGGGCTCGGCTGAACTCTGTGAGGAGGGG 59.0 0.28
mm4__chr14 104.83 Mbp_chr14_104825810_F Primer3: not found at this Tm N.d. 0.28
mm4__chr14 104.83 Mbp_chr14_104825810_R Primer3: not found at this Tm N.d. 0.28
mm4__chr12 124.57 Mbp_chr12_124565212_F TCGTCGGCAGCGTCAGTATTGATCCCCAGCAGCG 59.8 0.28
mm4__chr12 124.57 Mbp_chr12_124565212_R GTCTCGTGGGCTCGGGAAATTCACTGCTGCCACCG 60.1 0.28
mm4__chr4 7.53 Mbp_chr4_7530985_F TCGTCGGCAGCGTCCGAACTCTGGTACCTGCCTG 60.1 0.28
mm4__chr4 7.53 Mbp_chr4_7530985_R GTCTCGTGGGCTCGGCCCTCTCTGGACTCTGAGCT 60.0 0.28
mm4__chr1 21.90 Mbp_chr1_21904662_F TCGTCGGCAGCGTCATTAGGTCTCCCCTGGTGGC 60.9 0.26
mm4__chr1 21.90 Mbp_chr1_21904662_R GTCTCGTGGGCTCGGCCAGAAGCTGTTCCCGCTTA 60.0 0.26
mm4__chr8 63.14 Mbp_chr8_63144013_F Primer3: not found at this Tm N.d. 0.26
mm4__chr8 63.14 Mbp_chr8_63144013_R Primer3: not found at this Tm N.d. 0.26
mm4__chr11 76.65 Mbp_chr11_76653183_F TCGTCGGCAGCGTCAGGGCAGTCAAGTCACCAAG 59.8 0.25
mm4__chr11 76.65 Mbp_chr11_76653183_R GTCTCGTGGGCTCGGGGAGAATGGGGCCTGAACTC 60.1 0.25
mm4__chr10 85.79 Mbp_chr10_85787238_F TCGTCGGCAGCGTCCAGTCCTGGAAGGGTTTGGT 59.5 0.25
mm4__chr10 85.79 Mbp_chr10_85787238_R GTCTCGTGGGCTCGGGAGTGCATGGCCAAGATGGA 60.3 0.25
mm4__chr22 23.39 Mbp_chr22_23394377_F TCGTCGGCAGCGTCGGCATCTGAAAAGTGGCTGC 60.1 0.24
mm4__chr22 23.39 Mbp_chr22_23394377_R GTCTCGTGGGCTCGGGGAAACCCTCCTCAGACAGG 59.3 0.24
mm4__chr22 20.15 Mbp_chr22_20149023_F TCGTCGGCAGCGTCGAAACCCTCCTCAGACAGGG 59.3 0.24
mm4__chr22 20.15 Mbp_chr22_20149023_R GTCTCGTGGGCTCGGCATCGGAAAAGTGGCTGCAG 59.8 0.24
mm3__chr22 38.14 Mbp_chr22_38143532_F TCGTCGGCAGCGTCCTCGTACAAGAGATGGGCCC 59.8 0.24
mm3__chr22 38.14 Mbp_chr22_38143532_R GTCTCGTGGGCTCGGTCCGTGTTCTGTGACCCTTT 59.1 0.24
mm4__chr20 62.35 Mbp_chr20_62353610_F TCGTCGGCAGCGTCTGCAGATGGAGCAAGCTAGG 59.8 0.24
mm4__chr20 62.35 Mbp_chr20_62353610_R GTCTCGTGGGCTCGGGACCACTGTCTGGCCACC 59.9 0.24
mm4__chr10 29.74 Mbp_chr10_29735271_F TCGTCGGCAGCGTCCCACGCATCGGGTTCAAAC 59.7 0.24
mm4__chr10 29.74 Mbp_chr10_29735271_R GTCTCGTGGGCTCGGTCAGAACTACGACATCGCCC 59.5 0.24
mm4__chr14 99.96 Mbp_chr14_99956114_F Primer3: not found at this Tm N.d. 0.24
mm4__chr14 99.96 Mbp_chr14_99956114_R Primer3: not found at this Tm N.d. 0.24
mm4__chr21 42.25 Mbp_chr21_42253200_F TCGTCGGCAGCGTCAAGGCTGTTCCCAGGGATTG 59.9 0.24
mm4__chr21 42.25 Mbp_chr21_42253200_R GTCTCGTGGGCTCGGCTGCCTTAGTTGGAGCTGCT 60.0 0.24
mm4__chr16 53.36 Mbp_chr16_53358817_F TCGTCGGCAGCGTCAAGGGCCCTAGTCCAGTTTG 59.3 0.23
mm4__chr16 53.36 Mbp_chr16_53358817_R GTCTCGTGGGCTCGGCTACAGCCATGTCCCTGCC 60.1 0.23
mm4__chr2 235.55 Mbp_chr2_235551137_F TCGTCGGCAGCGTCTCTGAGGAAGAGAAGTGGGG 58.0 0.23
mm4__chr2 235.55 Mbp_chr2_235551137_R GTCTCGTGGGCTCGGATGAAGACCTTTCCCGGGTG 59.6 0.23
mm3__chr9 34.62 Mbp_chr9_34621247_F TCGTCGGCAGCGTCGGGTTACTGGAGGAAGAACCC 59.7 0.23
mm3__chr9 34.62 Mbp_chr9_34621247_R GTCTCGTGGGCTCGGCTCCCATGCCATGTGGACTT 60.0 0.23
mm4__chr16 5.51 Mbp_chr16_5508216_F Primer3: not found at this Tm N.d. 0.23
mm4__chr16 5.51 Mbp_chr16_5508216_R Primer3: not found at this Tm N.d. 0.23
mm4__chr1 24.69 Mbp_chr1_24686007_F Primer3: not found at this Tm N.d. 0.22
mm4__chr1 24.69 Mbp_chr1_24686007_R Primer3: not found at this Tm N.d. 0.22
mm4__chr7 44.23 Mbp_chr7_44229413_F TCGTCGGCAGCGTCAGCAGGACCCAGGCTACTTA 59.9 0.22
mm4__chr7 44.23 Mbp_chr7_44229413_R GTCTCGTGGGCTCGGCCGACATCCTGAGCTCAGTG 60.1 0.22
mm4__chr6 25.63 Mbp_chr6_25627802_F TCGTCGGCAGCGTCACCTGCAGAGCCTCATGTTC 60.0 0.22
mm4__chr6 25.63 Mbp_chr6_25627802_R GTCTCGTGGGCTCGGGCTGAGCTTCTTTTCCACCC 59.1 0.22
mm4__chr12 94.19 Mbp_chr12_94189924_F Primer3: not found at this Tm N.d. 0.21
mm4__chr12 94.19 Mbp_chr12_94189924_R Primer3: not found at this Tm N.d. 0.21
mm4__chr10 116.86 Mbp_chr10_116856544_F TCGTCGGCAGCGTCTCTGTGGTGATCTCGACTCA 57.8 0.21
mm4__chr10 116.86 Mbp_chr10_116856544_R GTCTCGTGGGCTCGGGATGGTGCTGTTGACCCACT 60.2 0.21
mm4__chr8 111.47 Mbp_chr8_111470626_F Primer3: not found at this Tm N.d. 0.21
mm4__chr8 111.47 Mbp_chr8_111470626_R Primer3: not found at this Tm N.d. 0.21
mm4__chr19 7.91 Mbp_chr19_7908206_F TCGTCGGCAGCGTCGTGGGAGGTGACGTGGTG 59.9 0.21
mm4__chr19 7.91 Mbp_chr19_7908206_R GTCTCGTGGGCTCGGCAGCGTTCATGACTGGCCTA 60.1 0.21
mm4__chr18 24.00 Mbp_chr18_24003105_F Primer3: not found at this Tm N.d. 0.20
mm4__chr18 24.00 Mbp_chr18_24003105_R Primer3: not found at this Tm N.d. 0.20
mm4__chr6 138.46 Mbp_chr6_138464250_F TCGTCGGCAGCGTCCTGACAGAGGCTGAGAGGC 59.4 0.20
mm4__chr6 138.46 Mbp_chr6_138464250_R GTCTCGTGGGCTCGGGACACCAGGGTCTGCCAAA 59.8 0.20
mm4__chr11 703.01 Kbp_chr11_703014_F Primer3: not found at this Tm N.d. 0.20
mm4__chr11 703.01 Kbp_chr11_703014_R Primer3: not found at this Tm N.d. 0.20
mm4__chr2 8.59 Mbp_chr2_8593932_F TCGTCGGCAGCGTCCTCCAACGTGACAAGAGGGG 60.3 0.20
mm4__chr2 8.59 Mbp_chr2_8593932_R GTCTCGTGGGCTCGGGAGGCATGAGGATCACCAGG 59.8 0.20
mm4__chr5 133.79 Mbp_chr5_133789168_F TCGTCGGCAGCGTCCACATGCTAACCTGAGCCCA 60.0 0.20
mm4__chr5 133.79 Mbp_chr5_133789168_R GTCTCGTGGGCTCGGGGTGAGGTTCCCTTCGGATG 60.1 0.20
mm4__chr8 81.62 Mbp_chr8_81621315_F TCGTCGGCAGCGTCATTGGCCACTGTGCCTTACA 59.8 0.20
mm4__chr8 81.62 Mbp_chr8_81621315_R GTCTCGTGGGCTCGGATGGTCATGGAGAGGGAAGC 59.1 0.20
mm4__chr3 122.68 Mbp_chr3_122681181_F TCGTCGGCAGCGTCCCTCCAGGGAAATGGCGG 60.1 0.20
mm4__chr3 122.68 Mbp_chr3_122681181_R GTCTCGTGGGCTCGGTGCGCTCGGAATCCATTCTG 60.8 0.20
mm4__chr5 80.50 Mbp_chr5_80503278_F Primer3: not found at this Tm N.d. 0.20
mm4__chr5 80.50 Mbp_chr5_80503278_R Primer3: not found at this Tm N.d. 0.20
mm4__chr14 101.15 Mbp_chr14_101151541_F TCGTCGGCAGCGTCGTGGGGCAGAGTGAGTCATG 60.3 0.20
mm4__chr14 101.15 Mbp_chr14_101151541_R GTCTCGTGGGCTCGGGAAGCAAGCCCTGGAGTGTT 60.5 0.20
mm4__chr17 76.16 Mbp_chr17_76156377_F TCGTCGGCAGCGTCGGGACACAACAGGACATGGA 59.6 0.20
mm4__chr17 76.16 Mbp_chr17_76156377_R GTCTCGTGGGCTCGGGTGTCGCCTTTCCTCCTCAT 59.7 0.20
mm4__chr16 1.92 Mbp_chr16_1918270_F Primer3: not found at this Tm N.d. 0.19
mm4__chr16 1.92 Mbp_chr16_1918270_R Primer3: not found at this Tm N.d. 0.19
mm4__chr5 2.27 Mbp_chr5_2271666_F Primer3: not found at this Tm N.d. 0.19
mm4__chr5 2.27 Mbp_chr5_2271666_R Primer3: not found at this Tm N.d. 0.19
mm4__chr8 143.04 Mbp_chr8_143042968_F TCGTCGGCAGCGTCGCCCAGAGGACAAGGAGTTT 59.5 0.19
mm4__chr8 143.04 Mbp_chr8_143042968_R GTCTCGTGGGCTCGGAGCTGCCTCACTGAATTGCA 60.2 0.19
mm4__chr3 98.32 Mbp_chr3_98322903_F Primer3: not found at this Tm N.d. 0.19
mm4__chr3 98.32 Mbp_chr3_98322903_R Primer3: not found at this Tm N.d. 0.19
mm4__chr2 238.80 Mbp_chr2_238796505_F TCGTCGGCAGCGTCCTGCAGGTCCCCTTCATGTC 60.3 0.19
mm4__chr2 238.80 Mbp_chr2_238796505_R GTCTCGTGGGCTCGGGGGCACAGAAGGGAAAGGTT 60.1 0.19
mm4__chr1 151.81 Mbp_chr1_151809584_F Primer3: not found at this Tm N.d. 0.19
mm4__chr1 151.81 Mbp_chr1_151809584_R Primer3: not found at this Tm N.d. 0.19
mm3__chr15 90.20 Mbp_chr15_90201079_F TCGTCGGCAGCGTCAAACCAACGCCACTAGCAGA 59.8 0.19
mm3__chr15 90.20 Mbp_chr15_90201079_R GTCTCGTGGGCTCGGCACTGCTCAGACCCTCCCA 60.9 0.19
mm4__chr18 79.34 Mbp_chr18_79337799_F TCGTCGGCAGCGTCATGCCATTTCCCAGGGATGG 60.1 0.19
mm4__chr18 79.34 Mbp_chr18_79337799_R GTCTCGTGGGCTCGGAGGAATGGCTGCAGACATCT 59.0 0.19
mm4__chr6 31.93 Mbp_chr6_31933899_F TCGTCGGCAGCGTCCTGACTCCTGTGTGGCTCTC 59.7 0.19
mm4__chr6 31.93 Mbp_chr6_31933899_R GTCTCGTGGGCTCGGCCCTTTGTCTTCTGGGTGGG 60.2 0.19
mm4__chr20 3.82 Mbp_chr20_3824125_F TCGTCGGCAGCGTCGAACCTGCTACTGGCTGAGG 60.1 0.19
mm4__chr20 3.82 Mbp_chr20_3824125_R GTCTCGTGGGCTCGGCAGGAAGCGGGTGAGGATG 60.1 0.19
mm4__chr21 43.95 Mbp_chr21_43947158_F TCGTCGGCAGCGTCTCATCCTCCCCTCGTCCTG 60.0 0.19
mm4__chr21 43.95 Mbp_chr21_43947158_R GTCTCGTGGGCTCGGCAAGTTGTGTCTGCCACTGC 59.9 0.19
mm3__chr4 148.50 Mbp_chr4_148504570_F TCGTCGGCAGCGTCAGGGCTACATGATGAGGGGA 59.7 0.19
mm3__chr4 148.50 Mbp_chr4_148504570_R GTCTCGTGGGCTCGGCAGACCACTGGCTTACCTCC 59.7 0.19
mm4__chr14 74.33 Mbp_chr14_74328207_F TCGTCGGCAGCGTCAGTACCTCCAACTTCCTGGGT 60.1 0.18
mm4__chr14 74.33 Mbp_chr14_74328207_R GTCTCGTGGGCTCGGGAAACCATGGCCACCTCTGA 59.9 0.18
mm4__chr12 110.37 Mbp_chr12_110370605_F TCGTCGGCAGCGTCCTGTGCTGCCAATGACCTTC 59.4 0.18
mm4__chr12 110.37 Mbp_chr12_110370605_R GTCTCGTGGGCTCGGTCTGCCGTCTAGACCCCTC 60.0 0.18
mm4__chr16 29.98 Mbp_chr16_29977251_F TCGTCGGCAGCGTCAGAGTGACTTTGCGGGCTC 60.0 0.18
mm4__chr16 29.98 Mbp_chr16_29977251_R GTCTCGTGGGCTCGGCCTCCTCCCTCAACCTCTCA 59.9 0.18
mm4__chr16 88.73 Mbp_chr16_88726526_F TCGTCGGCAGCGTCTCCATCATCTCCTTGGCTGC 59.8 0.18
mm4__chr16 88.73 Mbp_chr16_88726526_R GTCTCGTGGGCTCGGTATGCACCGTCAAGGGCTAC 59.8 0.18
mm4__chr11 61.74 Mbp_chr11_61739689_F TCGTCGGCAGCGTCCAGAGCAGTGCTGGTAGGTT 59.6 0.17
mm4__chr11 61.74 Mbp_chr11_61739689_R GTCTCGTGGGCTCGGCATCTTCTCCCAGCCTCCAC 59.8 0.17
mm4__chr10 16.44 Mbp_chr10_16437006_F TCGTCGGCAGCGTCAGGGCACAGGGGAGACTAAA 60.1 0.17
mm4__chr10 16.44 Mbp_chr10_16437006_R GTCTCGTGGGCTCGGCCAAGAAGCCTTACCTCGGG 60.1 0.17
mm4__chr1 111.90 Mbp_chr1_111904921_F TCGTCGGCAGCGTCAGACTCAGGATGCACTTGGG 59.3 0.17
mm4__chr1 111.90 Mbp_chr1_111904921_R GTCTCGTGGGCTCGGCTCCATCACTGACAGGGCTG 60.1 0.17
mm4__chr22 39.39 Mbp_chr22_39388829_F TCGTCGGCAGCGTCGCTGTCATCCCTTAGTGGCC 60.4 0.17
mm4__chr22 39.39 Mbp_chr22_39388829_R GTCTCGTGGGCTCGGGAGTGGAGCGAACACCTGAA 59.9 0.17
mm4__chr1 115.68 Mbp_chr1_115679385_F TCGTCGGCAGCGTCGGAGGACTGTGAGAGCCATG 59.8 0.17
mm4__chr1 115.68 Mbp_chr1_115679385_R GTCTCGTGGGCTCGGATAAGCCAAGTCCACAGCCC 60.0 0.17
mm4__chr2 28.64 Mbp_chr2_28643113_F TCGTCGGCAGCGTCATAGCCACTCTCTTCACCGC 59.5 0.16
mm4__chr2 28.64 Mbp_chr2_28643113_R GTCTCGTGGGCTCGGGGCCCCAAGAAGTTGTGACT 60.1 0.16
mm4__chr16 2.34 Mbp_chr16_2341664_F TCGTCGGCAGCGTCGAGGTGGTCCGGAGAGGG 60.7 0.16
mm4__chr16 2.34 Mbp_chr16_2341664_R GTCTCGTGGGCTCGGCTGGGACCGCCTAATGTGAC 60.4 0.16
mm4__chr10 3.91 Mbp_chr10_3913641_F Primer3: not found at this Tm N.d. 0.16
mm4__chr10 3.91 Mbp_chr10_3913641_R Primer3: not found at this Tm N.d. 0.16
mm4__chr3 136.63 Mbp_chr3_136630366_F TCGTCGGCAGCGTCGTAGCCGGAACACCTCAACA 59.9 0.16
mm4__chr3 136.63 Mbp_chr3_136630366_R GTCTCGTGGGCTCGGACCATACTCTTTCTCTACCCCT 57.3 0.16
mm4__chr10 45.37 Mbp_chr10_45374488_F TCGTCGGCAGCGTCCTGAGGTGCGTCCGGGAC 62.4 0.16
mm4__chr10 45.37 Mbp_chr10_45374488_R GTCTCGTGGGCTCGGAGTTCTGCCCTCCCTGACA 60.1 0.16
mm4__chr7 332.54 Kbp_chr7_332543_F TCGTCGGCAGCGTCGTCCCGTGGATGCTGACAG 60.4 0.16
mm4__chr7 332.54 Kbp_chr7_332543_R GTCTCGTGGGCTCGGGGTGTGTGGACCAGGGAGA 60.8 0.16
mm4__chr4 8.03 Mbp_chr4_8025726_F TCGTCGGCAGCGTCCTCTAGGACCCACAGAGGCT 60.0 0.16
mm4__chr4 8.03 Mbp_chr4_8025726_R GTCTCGTGGGCTCGGGTCCCAACTGTGAGTCCCTG 59.9 0.16
mm3__chr11_KI270721v1_random 53.14 Kbp_chr11_KI270721v1_random_53139_F TCGTCGGCAGCGTCTTTTCTAACTGGGGTGGCCC 59.8 0.16
mm3__chr11_KI270721v1_random 53.14 Kbp_chr11_KI270721v1_random_53139_R GTCTCGTGGGCTCGGGTGCTGGGTGAGAGAGACG 59.7 0.16
mm3__chr11 2.00 Mbp_chr11_1997957_F TCGTCGGCAGCGTCTTTTCTAACTGGGGTGGCCC 59.8 0.16
mm3__chr11 2.00 Mbp_chr11_1997957_R GTCTCGTGGGCTCGGGTGCTGGGTGAGAGAGACG 59.7 0.16
mm3__chr16 27.81 Mbp_chr16_27811500_F TCGTCGGCAGCGTCCCCTCCTCATCCCGGAACA 60.6 0.16
mm3__chr16 27.81 Mbp_chr16_27811500_R GTCTCGTGGGCTCGGGAGGATGCTGGAAGCCCTTT 60.0 0.16
mm3__chr13 45.83 Mbp_chr13_45832051_F TCGTCGGCAGCGTCATGAGTGCTAGGGAGAGCCA 60.0 0.16
mm3__chr13 45.83 Mbp_chr13_45832051_R GTCTCGTGGGCTCGGGCACCCCACACCTCTCTTC 60.0 0.16
mm4__chr8 30.98 Mbp_chr8_30977615_F Primer3: not found at this Tm N.d. 0.16
mm4__chr8 30.98 Mbp_chr8_30977615_R Primer3: not found at this Tm N.d. 0.16
mm4__chr2 234.95 Mbp_chr2_234952480_F Primer3: not found at this Tm N.d. 0.16
mm4__chr2 234.95 Mbp_chr2_234952480_R Primer3: not found at this Tm N.d. 0.16
mm4__chr17 46.02 Mbp_chr17_46021003_F TCGTCGGCAGCGTCCCACCAGGCCACTCAAGTAG 60.0 0.15
mm4__chr17 46.02 Mbp_chr17_46021003_R GTCTCGTGGGCTCGGGGGTGCCCATCTGTTCAGAA 59.9 0.15
mm4__chr9 136.84 Mbp_chr9_136843432_F TCGTCGGCAGCGTCACCTTCTGAGCGAAATGGGG 60.0 0.15
mm4__chr9 136.84 Mbp_chr9_136843432_R GTCTCGTGGGCTCGGCTCCTCTGAGCTGCGCAC 60.5 0.15
mm4__chr1 247.11 Mbp_chr1_247111744_F TCGTCGGCAGCGTCCTTGGAGCTCGTAGGCACC 60.1 0.15
mm4__chr1 247.11 Mbp_chr1_247111744_R GTCTCGTGGGCTCGGCTGACCCCACACCCTCCC 61.3 0.15
mm4__chr2 130.92 Mbp_chr2_130920987_F Primer3: not found at this Tm N.d. 0.15
mm4__chr2 130.92 Mbp_chr2_130920987_R Primer3: not found at this Tm N.d. 0.15
mm4__chr4 147.86 Mbp_chr4_147862292_F Primer3: not found at this Tm N.d. 0.15
mm4__chr4 147.86 Mbp_chr4_147862292_R Primer3: not found at this Tm N.d. 0.15
mm4__chr17 36.71 Mbp_chr17_36706306_F TCGTCGGCAGCGTCCCTACGTGTGTGCTCCAAGT 59.9 0.15
mm4__chr17 36.71 Mbp_chr17_36706306_R GTCTCGTGGGCTCGGAGAAAAGACCCACCTGTGCT 59.1 0.15
mm4__chr9 134.97 Mbp_chr9_134967700_F TCGTCGGCAGCGTCTTTAGAGGGAGTGCGTCTGC 59.7 0.14
mm4__chr9 134.97 Mbp_chr9_134967700_R GTCTCGTGGGCTCGGCGTTTCCTGCTCTGAGCTGA 60.0 0.14
mm4__chr16 87.69 Mbp_chr16_87687531_F TCGTCGGCAGCGTCAAGGCAGGACCATGAAGGAG 59.3 0.14
mm4__chr16 87.69 Mbp_chr16_87687531_R GTCTCGTGGGCTCGGTCTTCCTGTGCCTTCAAGCC 60.2 0.14
mm4__chr3 98.02 Mbp_chr3_98024575_F TCGTCGGCAGCGTCGCCTATTGTGCTTCCCTTGG 58.8 0.14
mm4__chr3 98.02 Mbp_chr3_98024575_R GTCTCGTGGGCTCGGGGGTGAGAAGAAAGATCCTCTGG 60.1 0.14
mm4__chr8 23.53 Mbp_chr8_23528951_F TCGTCGGCAGCGTCGAAGTGAAGTTTTGCGCCCC 60.3 0.14
mm4__chr8 23.53 Mbp_chr8_23528951_R GTCTCGTGGGCTCGGGCTGTCCCCATCCATCCTC 59.5 0.14
mm4__chr16 11.49 Mbp_chr16_11494139_F Primer3: not found at this Tm N.d. 0.14
mm4__chr16 11.49 Mbp_chr16_11494139_R Primer3: not found at this Tm N.d. 0.14
mm4__chr17 82.78 Mbp_chr17_82784644_F TCGTCGGCAGCGTCTTGCCTTGGGTCTCACTGAG 59.6 0.14
mm4__chr17 82.78 Mbp_chr17_82784644_R GTCTCGTGGGCTCGGGTCCTTGGTCATGTGGGAGG 60.0 0.14
mm4__chr22 25.12 Mbp_chr22_25119903_F Primer3: not found at this Tm N.d. 0.14
mm4__chr22 25.12 Mbp_chr22_25119903_R Primer3: not found at this Tm N.d. 0.14
mm4__chr16 29.75 Mbp_chr16_29747264_F Primer3: not found at this Tm N.d. 0.14
mm4__chr16 29.75 Mbp_chr16_29747264_R Primer3: not found at this Tm N.d. 0.14
mm4__chr19 49.58 Mbp_chr19_49580588_F TCGTCGGCAGCGTCGGTCGCTTCTTCAACTGTGC 59.7 0.14
mm4__chr19 49.58 Mbp_chr19_49580588_R GTCTCGTGGGCTCGGGCAACTAGGGGCAGCTCTAC 60.1 0.14
mm4__chr13 83.45 Mbp_chr13_83452525_F Primer3: not found at this Tm N.d. 0.14
mm4__chr13 83.45 Mbp_chr13_83452525_R Primer3: not found at this Tm N.d. 0.14
mm4__chr1 175.73 Mbp_chr1_175726897_F Primer3: not found at this Tm N.d. 0.14
mm4__chr1 175.73 Mbp_chr1_175726897_R Primer3: not found at this Tm N.d. 0.14
mm4__chr18 36.53 Mbp_chr18_36532337_F TCGTCGGCAGCGTCAAACTCCAGGACCCATACGC 59.7 0.14
mm4__chr18 36.53 Mbp_chr18_36532337_R GTCTCGTGGGCTCGGGGTGCCACAGAAGCCACTAT 60.0 0.14
mm4__chr11 62.04 Mbp_chr11_62042410_F TCGTCGGCAGCGTCGTAAAGGCAGGTGGGGTGAG 60.3 0.13
mm4__chr11 62.04 Mbp_chr11_62042410_R GTCTCGTGGGCTCGGTCCAGCTGGCATTATAGGGC 59.5 0.13
mm4__chr4 24.65 Mbp_chr4_24650161_F TCGTCGGCAGCGTCTCTTTTGGTGGCTCAGTGGG 60.1 0.13
mm4__chr4 24.65 Mbp_chr4_24650161_R GTCTCGTGGGCTCGGCTCCTGAGGGGCACACAATT 59.9 0.13
mm4__chr3 197.03 Mbp_chr3_197027939_F TCGTCGGCAGCGTCCACCTCCATGCCACCGAG 60.1 0.13
mm4__chr3 197.03 Mbp_chr3_197027939_R GTCTCGTGGGCTCGGATGGGCTCACACAGAACTGC 60.6 0.13
mm4__chr12 124.45 Mbp_chr12_124453546_F Primer3: not found at this Tm N.d. 0.13
mm4__chr12 124.45 Mbp_chr12_124453546_R Primer3: not found at this Tm N.d. 0.13
mm4__chr14 93.71 Mbp_chr14_93706542_F TCGTCGGCAGCGTCTATGATGGGTTTGGGCCAGG 59.7 0.13
mm4__chr14 93.71 Mbp_chr14_93706542_R GTCTCGTGGGCTCGGTAGGCTTGGCTCAAGGGTTG 59.9 0.13
mm4__chr4 146.19 Mbp_chr4_146192870_F TCGTCGGCAGCGTCACAGGACAGTGGAGAGAGCC 60.9 0.13
mm4__chr4 146.19 Mbp_chr4_146192870_R GTCTCGTGGGCTCGGCCTGCCTACAGCTGTCATCC 60.1 0.13
mm4__chr2 207.46 Mbp_chr2_207463817_F Primer3: not found at this Tm N.d. 0.13
mm4__chr2 207.46 Mbp_chr2_207463817_R Primer3: not found at this Tm N.d. 0.13
mm4__chr16 381.96 Kbp_chr16_381961_F Primer3: not found at this Tm N.d. 0.13
mm4__chr16 381.96 Kbp_chr16_381961_R Primer3: not found at this Tm N.d. 0.13
mm4__chr13 113.08 Mbp_chr13_113080729_F TCGTCGGCAGCGTCCACCTGCTGTGTGTGAGGG 60.5 0.13
mm4__chr13 113.08 Mbp_chr13_113080729_R GTCTCGTGGGCTCGGTCCTCGTGGTGGGTATCTGT 59.9 0.13
mm3__chr12 109.83 Mbp_chr12_109828321_F TCGTCGGCAGCGTCTCCTGTGCGCCTGGTGTG 62.6 0.13
mm3__chr12 109.83 Mbp_chr12_109828321_R GTCTCGTGGGCTCGGCCTGAGCATTGGTTTCCCCT 59.9 0.13
mm4__chr5 81.41 Mbp_chr5_81410722_F Primer3: not found at this Tm N.d. 0.13
mm4__chr5 81.41 Mbp_chr5_81410722_R Primer3: not found at this Tm N.d. 0.13
mm4__chr2 120.34 Mbp_chr2_120344495_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 120.34 Mbp_chr2_120344495_R Primer3: not found at this Tm N.d. 0.12
mm4__chr18 37.34 Mbp_chr18_37336985_F TCGTCGGCAGCGTCGCTGCGTCCCACTTCATTTC 59.8 0.12
mm4__chr18 37.34 Mbp_chr18_37336985_R GTCTCGTGGGCTCGGGTAGCATGGAAGGGTCGACA 59.4 0.12
mm4__chr22 32.92 Mbp_chr22_32917908_F Primer3: not found at this Tm N.d. 0.12
mm4__chr22 32.92 Mbp_chr22_32917908_R Primer3: not found at this Tm N.d. 0.12
mm4__chr2 241.78 Mbp_chr2_241776877_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 241.78 Mbp_chr2_241776877_R Primer3: not found at this Tm N.d. 0.12
mm4__chr9 134.65 Mbp_chr9_134654856_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 134.65 Mbp_chr9_134654856_R Primer3: not found at this Tm N.d. 0.12
mm4__chr17 81.47 Mbp_chr17_81468256_F TCGTCGGCAGCGTCGACTAGGGGTCCAGGTGGG 60.6 0.12
mm4__chr17 81.47 Mbp_chr17_81468256_R GTCTCGTGGGCTCGGACCTAAGCCTGCCGTCAAC 60.0 0.12
mm4__chr9 129.10 Mbp_chr9_129101351_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 129.10 Mbp_chr9_129101351_R Primer3: not found at this Tm N.d. 0.12
mm4__chr4 105.15 Mbp_chr4_105146643_F Primer3: not found at this Tm N.d. 0.12
mm4__chr4 105.15 Mbp_chr4_105146643_R Primer3: not found at this Tm N.d. 0.12
mm4__chr2 24.92 Mbp_chr2_24920628_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 24.92 Mbp_chr2_24920628_R Primer3: not found at this Tm N.d. 0.12
mm4__chr2 108.58 Mbp_chr2_108584818_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 108.58 Mbp_chr2_108584818_R Primer3: not found at this Tm N.d. 0.12
mm4__chr14 55.13 Mbp_chr14_55125735_F TCGTCGGCAGCGTCATGTGGGTTTGGTGATGGCT 59.8 0.11
mm4__chr14 55.13 Mbp_chr14_55125735_R GTCTCGTGGGCTCGGGGTGCAAAACCCCTTCTCCA 60.4 0.11
mm4__chr14 100.50 Mbp_chr14_100503682_F TCGTCGGCAGCGTCCCCCGGCTTCACTTACACAT 60.0 0.11
mm4__chr14 100.50 Mbp_chr14_100503682_R GTCTCGTGGGCTCGGCAACGGGAATCCAGCAGTCT 60.0 0.11
mm4__chr12 27.78 Mbp_chr12_27779984_F TCGTCGGCAGCGTCGATCCAGCCGGCAGGTCA 61.7 0.11
mm4__chr12 27.78 Mbp_chr12_27779984_R GTCTCGTGGGCTCGGGAATTCCAGCTCCCAGCCAG 60.7 0.11
mm4__chr3 50.37 Mbp_chr3_50371931_F TCGTCGGCAGCGTCGACAACTCAAGCCAGTGGGA 59.8 0.11
mm4__chr3 50.37 Mbp_chr3_50371931_R GTCTCGTGGGCTCGGGTCCAGCCTAGTCCAGCAAA 59.6 0.11
mm4__chr1 21.26 Mbp_chr1_21260925_F TCGTCGGCAGCGTCCTTACAGCCAACTCCTGGGG 60.0 0.11
mm4__chr1 21.26 Mbp_chr1_21260925_R GTCTCGTGGGCTCGGAAGGTCTGAGAAGCCACTGC 59.9 0.11
mm4__chr1 109.79 Mbp_chr1_109792708_F TCGTCGGCAGCGTCCCAAGGGAGTCAGCATGGAG 60.1 0.11
mm4__chr1 109.79 Mbp_chr1_109792708_R GTCTCGTGGGCTCGGGGGTTCCGTGCCCATCTAAA 60.0 0.11
mm4__chr6 43.48 Mbp_chr6_43477555_F TCGTCGGCAGCGTCGCCTCAGATGACGCGACA 59.8 0.11
mm4__chr6 43.48 Mbp_chr6_43477555_R GTCTCGTGGGCTCGGCCCACAACAAACTTTCCGCC 60.2 0.11
mm4__chr9 92.74 Mbp_chr9_92738028_F Primer3: not found at this Tm N.d. 0.11
mm4__chr9 92.74 Mbp_chr9_92738028_R Primer3: not found at this Tm N.d. 0.11
mm4__chr11 8.39 Mbp_chr11_8386542_F TCGTCGGCAGCGTCCCTCTCTTCTCCTGGGACCA 59.9 0.11
mm4__chr11 8.39 Mbp_chr11_8386542_R GTCTCGTGGGCTCGGCATAGGCTCCGCGGATTCTG 60.6 0.11
mm4__chr10 60.29 Mbp_chr10_60287344_F TCGTCGGCAGCGTCAGCTTTCCCTACAGCTGAAAA 57.4 0.11
mm4__chr10 60.29 Mbp_chr10_60287344_R GTCTCGTGGGCTCGGGTGCCAACTACAGCCTCCTG 60.6 0.11
mm4__chr22 45.65 Mbp_chr22_45648576_F TCGTCGGCAGCGTCGTTCCAACTGCACTCCGAGA 59.9 0.11
mm4__chr22 45.65 Mbp_chr22_45648576_R GTCTCGTGGGCTCGGAGCTGAGATCAGGACGAGGT 60.0 0.11
mm3__chr2 37.67 Mbp_chr2_37671345_F TCGTCGGCAGCGTCTAGATGGGAAAGCGATGCCC 59.8 0.11
mm3__chr2 37.67 Mbp_chr2_37671345_R GTCTCGTGGGCTCGGCTGTCTGTGAGTCTGTCCCG 59.7 0.11
mm4__chr1 8.22 Mbp_chr1_8217376_F TCGTCGGCAGCGTCGTCAGCCAGGCCAGCTTG 61.0 0.11
mm4__chr1 8.22 Mbp_chr1_8217376_R GTCTCGTGGGCTCGGGCTGCGGGGAGAGAAGCC 62.8 0.11
mm4__chr9 134.27 Mbp_chr9_134272809_F Primer3: not found at this Tm N.d. 0.11
mm4__chr9 134.27 Mbp_chr9_134272809_R Primer3: not found at this Tm N.d. 0.11
mm3__chr1 159.98 Mbp_chr1_159983899_F TCGTCGGCAGCGTCGGGAAAGAGACCCACGTCAC 60.3 0.10
mm3__chr1 159.98 Mbp_chr1_159983899_R GTCTCGTGGGCTCGGATCATATCTGCAGCCCTGGC 59.9 0.10
mm4__chr10 92.57 Mbp_chr10_92574162_F TCGTCGGCAGCGTCGGTAGCTGCTCCGGGTTTT 60.0 0.10
mm4__chr10 92.57 Mbp_chr10_92574162_R GTCTCGTGGGCTCGGAAACCCCTGTCCCTCCAGT 60.0 0.10
mm4__chr7 30.50 Mbp_chr7_30503563_F Primer3: not found at this Tm N.d. 0.10
mm4__chr7 30.50 Mbp_chr7_30503563_R Primer3: not found at this Tm N.d. 0.10
mm4__chr19 55.45 Mbp_chr19_55454561_F TCGTCGGCAGCGTCTGGCCAGTGGAGTCTGAGAA 60.4 0.10
mm4__chr19 55.45 Mbp_chr19_55454561_R GTCTCGTGGGCTCGGGATAGAAGCCCCTTGACCCG 59.8 0.10
mm4__chr9 133.38 Mbp_chr9_133376657_F TCGTCGGCAGCGTCGTCGCAACCCTGGAGCTAC 60.4 0.10
mm4__chr9 133.38 Mbp_chr9_133376657_R GTCTCGTGGGCTCGGCGGGTTCAACCTAGGAGCTG 60.1 0.10
mm4__chr14 24.13 Mbp_chr14_24131388_F TCGTCGGCAGCGTCCATACTACCACACACCCGAGG 59.8 0.10
mm4__chr14 24.13 Mbp_chr14_24131388_R GTCTCGTGGGCTCGGCAGTGACAGGTGTGGCAGAC 60.8 0.10
mm4__chr8 94.39 Mbp_chr8_94389239_F Primer3: not found at this Tm N.d. 0.10
mm4__chr8 94.39 Mbp_chr8_94389239_R Primer3: not found at this Tm N.d. 0.10
mm4__chr17 6.56 Mbp_chr17_6556123_F TCGTCGGCAGCGTCGACATCCCCTTCGGTGGC 60.1 0.10
mm4__chr17 6.56 Mbp_chr17_6556123_R GTCTCGTGGGCTCGGGACACCGCGTCCCCTTCTC 62.6 0.10
mm4__chr7 86.21 Mbp_chr7_86209104_F Primer3: not found at this Tm N.d. 0.10
mm4__chr7 86.21 Mbp_chr7_86209104_R Primer3: not found at this Tm N.d. 0.10
mm4__chr5 135.54 Mbp_chr5_135536013_F TCGTCGGCAGCGTCTAAGTACCCGGCGCAACAAT 60.0 0.10
mm4__chr5 135.54 Mbp_chr5_135536013_R GTCTCGTGGGCTCGGGCCTCCCGCGAGCATAAATT 61.1 0.10
mm4__chr16 1.51 Mbp_chr16_1508090_F TCGTCGGCAGCGTCCCCTCCTTTGGTCCTGATCC 59.4 0.10
mm4__chr16 1.51 Mbp_chr16_1508090_R GTCTCGTGGGCTCGGGTTGCCAGGACTGCACCG 61.3 0.10
mm4__chr14 90.74 Mbp_chr14_90743862_F TCGTCGGCAGCGTCTGAGAATCTGAAACAGGCATGT 57.9 0.10
mm4__chr14 90.74 Mbp_chr14_90743862_R GTCTCGTGGGCTCGGGAGCTCGGGGTTCTAAGCTC 59.8 0.10
mm4__chr6 170.03 Mbp_chr6_170027083_F Primer3: not found at this Tm N.d. 0.10
mm4__chr6 170.03 Mbp_chr6_170027083_R Primer3: not found at this Tm N.d. 0.10
mm4__chr1 46.41 Mbp_chr1_46410959_F TCGTCGGCAGCGTCCGAGTCTGGGTCTGGGTAGT 60.3 0.09
mm4__chr1 46.41 Mbp_chr1_46410959_R GTCTCGTGGGCTCGGCTGAAGGCCACAAAGGTTGC 59.9 0.09
mm4__chr9 129.48 Mbp_chr9_129483250_F TCGTCGGCAGCGTCCCGGATCTGAGCTTCGTCAT 59.6 0.09
mm4__chr9 129.48 Mbp_chr9_129483250_R GTCTCGTGGGCTCGGCGGTGAAATTCTGCCAGTGG 59.4 0.09
mm4__chr4 118.00 Mbp_chr4_118004333_F Primer3: not found at this Tm N.d. 0.09
mm4__chr4 118.00 Mbp_chr4_118004333_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 43.99 Mbp_chr17_43994858_F TCGTCGGCAGCGTCGGATCCGAGACAGAGCCCT 60.4 0.09
mm4__chr17 43.99 Mbp_chr17_43994858_R GTCTCGTGGGCTCGGTGCTCATGCTTACCGCCTTT 60.3 0.09
mm4__chr21 45.03 Mbp_chr21_45032186_F Primer3: not found at this Tm N.d. 0.09
mm4__chr21 45.03 Mbp_chr21_45032186_R Primer3: not found at this Tm N.d. 0.09
mm4__chr6 41.68 Mbp_chr6_41679791_F TCGTCGGCAGCGTCAAAGGCCAATACCTGCACCA 59.8 0.09
mm4__chr6 41.68 Mbp_chr6_41679791_R GTCTCGTGGGCTCGGTCCCTCTCCTCTCACCCATG 60.0 0.09
mm4__chr17 77.96 Mbp_chr17_77959424_F TCGTCGGCAGCGTCGCCCTTTGTTCGGCCGAG 61.4 0.09
mm4__chr17 77.96 Mbp_chr17_77959424_R GTCTCGTGGGCTCGGTCTCACACTAGCACACACGC 60.3 0.09
mm4__chr11 1.37 Mbp_chr11_1370765_F TCGTCGGCAGCGTCATACAGGGCAATGGAGCAGG 59.8 0.09
mm4__chr11 1.37 Mbp_chr11_1370765_R GTCTCGTGGGCTCGGGATGGGACTCGGAGCAGAAC 60.1 0.09
mm4__chr19 15.12 Mbp_chr19_15117052_F TCGTCGGCAGCGTCATCTCCAGTTCAGGCCTCCT 59.9 0.09
mm4__chr19 15.12 Mbp_chr19_15117052_R GTCTCGTGGGCTCGGCACCGTTGCATCTGTGTTGG 60.0 0.09
mm4__chr4 965.54 Kbp_chr4_965537_F TCGTCGGCAGCGTCATGTGACTCACGGACACCAC 59.9 0.09
mm4__chr4 965.54 Kbp_chr4_965537_R GTCTCGTGGGCTCGGCTGCCTTTGGGGTGAGATGT 59.9 0.09
mm3__chr20 34.87 Mbp_chr20_34872847_F TCGTCGGCAGCGTCCTGGCTGGCCTCGTTCTC 60.1 0.08
mm3__chr20 34.87 Mbp_chr20_34872847_R GTCTCGTGGGCTCGGGGCTGTCGCGAAGATCAGG 60.8 0.08
mm3__chr16 81.04 Mbp_chr16_81035808_F Primer3: not found at this Tm N.d. 0.08
mm3__chr16 81.04 Mbp_chr16_81035808_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 24.83 Mbp_chr3_24830505_F TCGTCGGCAGCGTCCTAAGGATGCTCGGGTGCTG 60.5 0.08
mm4__chr3 24.83 Mbp_chr3_24830505_R GTCTCGTGGGCTCGGCGACCATCACTTCGCCTCTG 60.8 0.08
mm4__chr16 67.55 Mbp_chr16_67546051_F TCGTCGGCAGCGTCGAGGATGAGGACGTGCAGAC 60.1 0.08
mm4__chr16 67.55 Mbp_chr16_67546051_R GTCTCGTGGGCTCGGGGAGCAGATGGGAGGGTTTC 60.1 0.08
mm4__chr10 127.88 Mbp_chr10_127879606_F Primer3: not found at this Tm N.d. 0.08
mm4__chr10 127.88 Mbp_chr10_127879606_R Primer3: not found at this Tm N.d. 0.08
mm3__chr7 99.14 Mbp_chr7_99144071_F Primer3: not found at this Tm N.d. 0.08
mm3__chr7 99.14 Mbp_chr7_99144071_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 3.07 Mbp_chr1_3074112_F TCGTCGGCAGCGTCGGGAAGACGGTGCAGAGAAA 59.9 0.08
mm4__chr1 3.07 Mbp_chr1_3074112_R GTCTCGTGGGCTCGGCAAGTGCACTAGGGAGCCC 60.0 0.08
mm3__chr5 179.00 Mbp_chr5_178997060_F Primer3: not found at this Tm N.d. 0.08
mm3__chr5 179.00 Mbp_chr5_178997060_R Primer3: not found at this Tm N.d. 0.08
mm4__chr19 2.43 Mbp_chr19_2426785_F TCGTCGGCAGCGTCCCGAGATCCAAGCTGCACTG 61.0 0.08
mm4__chr19 2.43 Mbp_chr19_2426785_R GTCTCGTGGGCTCGGGGGTCCGCATGTACGTACTG 60.5 0.08
mm4__chr7 10.38 Mbp_chr7_10379294_F TCGTCGGCAGCGTCGTCCAAGAAGTGATCATTGCCC 59.5 0.08
mm4__chr7 10.38 Mbp_chr7_10379294_R GTCTCGTGGGCTCGGACAGGAATGCATGTGGGGTT 59.8 0.08
mm4__chr7 76.26 Mbp_chr7_76260918_F TCGTCGGCAGCGTCAGTCCGAATGCAGGTCAGTG 60.0 0.08
mm4__chr7 76.26 Mbp_chr7_76260918_R GTCTCGTGGGCTCGGGCCTTGGAATCTCTCCCACC 60.1 0.08
mm4__chr20 31.50 Mbp_chr20_31496954_F TCGTCGGCAGCGTCCCCGGAGAAACTGTCTGCAA 60.2 0.08
mm4__chr20 31.50 Mbp_chr20_31496954_R GTCTCGTGGGCTCGGCCTGGACAGATGCCAGATCC 59.8 0.08
mm4__chr19 954.37 Kbp_chr19_954365_F TCGTCGGCAGCGTCCCCAGCCTTGGTAATTAGGGG 60.1 0.08
mm4__chr19 954.37 Kbp_chr19_954365_R GTCTCGTGGGCTCGGGATGCCAAGAAAATGCGGGG 60.1 0.08
mm4__chr19 797.70 Kbp_chr19_797703_F Primer3: not found at this Tm N.d. 0.08
mm4__chr19 797.70 Kbp_chr19_797703_R Primer3: not found at this Tm N.d. 0.08
mm4__chr19 44.99 Mbp_chr19_44985971_F TCGTCGGCAGCGTCTACATGTGGGGTGGGAGGAT 59.9 0.08
mm4__chr19 44.99 Mbp_chr19_44985971_R GTCTCGTGGGCTCGGGGCTCTCCTCCCTAGGCTTA 59.8 0.08
mm4__chr1 205.53 Mbp_chr1_205532680_F Primer3: not found at this Tm N.d. 0.08
mm4__chr1 205.53 Mbp_chr1_205532680_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 16.09 Mbp_chr1_16089338_F TCGTCGGCAGCGTCGAGCCACTAGTCAGGACTGC 59.8 0.08
mm4__chr1 16.09 Mbp_chr1_16089338_R GTCTCGTGGGCTCGGACCTGGACTCTACCCACCTC 59.9 0.08
mm4__chr14 105.50 Mbp_chr14_105498879_F TCGTCGGCAGCGTCTGCTACGTCGACTACAGGGA 60.0 0.08
mm4__chr14 105.50 Mbp_chr14_105498879_R GTCTCGTGGGCTCGGGATCCAGATGAGACCAGGGC 59.6 0.08
mm4__chrY 14.83 Mbp_chrY_14830269_F TCGTCGGCAGCGTCGAAAACCCTGGTGGCTCTCT 59.5 0.08
mm4__chrY 14.83 Mbp_chrY_14830269_R GTCTCGTGGGCTCGGGGCTTCATTTCGCTTTGGCA 60.0 0.08
mm4__chrX 5.90 Mbp_chrX_5903303_F TCGTCGGCAGCGTCGGCTTCATTTCGCTTTGGCA 60.0 0.08
mm4__chrX 5.90 Mbp_chrX_5903303_R GTCTCGTGGGCTCGGGAAAACCCTGGTGGCTCTCT 59.5 0.08
mm4__chr1 52.59 Mbp_chr1_52587885_F Primer3: not found at this Tm N.d. 0.07
mm4__chr1 52.59 Mbp_chr1_52587885_R Primer3: not found at this Tm N.d. 0.07
mm4__chr5 119.27 Mbp_chr5_119268572_F Primer3: not found at this Tm N.d. 0.07
mm4__chr5 119.27 Mbp_chr5_119268572_R Primer3: not found at this Tm N.d. 0.07
mm4__chr3 194.88 Mbp_chr3_194877809_F TCGTCGGCAGCGTCTAGTCCGGCTTCTACCTGCT 60.0 0.07
mm4__chr3 194.88 Mbp_chr3_194877809_R GTCTCGTGGGCTCGGGGGAAAGAGGCTTGGCAAGT 60.5 0.07
mm4__chr1 159.84 Mbp_chr1_159840642_F TCGTCGGCAGCGTCTGAGAGAGGCAGATGGTGGA 59.9 0.07
mm4__chr1 159.84 Mbp_chr1_159840642_R GTCTCGTGGGCTCGGTTCCCCTGCACTTCTTGTCC 59.8 0.07
mm3__chrUn_KI270744v1 75.57 Kbp_chrUn_KI270744v1_75573_F TCGTCGGCAGCGTCGAGCTGCCCTGCAAAGAGTA 60.0 0.07
mm3__chrUn_KI270744v1 75.57 Kbp_chrUn_KI270744v1_75573_R GTCTCGTGGGCTCGGCCTGGTGGTAGCGAGCATAG 59.9 0.07
mm4__chr19 439.20 Kbp_chr19_439200_F Primer3: not found at this Tm N.d. 0.07
mm4__chr19 439.20 Kbp_chr19_439200_R Primer3: not found at this Tm N.d. 0.07
mm4__chr3 43.11 Mbp_chr3_43105612_F TCGTCGGCAGCGTCGAGGGCTCGAACTGGCAAG 60.7 0.07
mm4__chr3 43.11 Mbp_chr3_43105612_R GTCTCGTGGGCTCGGGTGAAGCGAGCTGAGGGAG 60.1 0.07
mm4__chr12 51.81 Mbp_chr12_51814454_F Primer3: not found at this Tm N.d. 0.07
mm4__chr12 51.81 Mbp_chr12_51814454_R Primer3: not found at this Tm N.d. 0.07
mm3__chr2 241.95 Mbp_chr2_241953839_F TCGTCGGCAGCGTCCAAAGGCCTGGAGCAGATGT 60.3 0.07
mm3__chr2 241.95 Mbp_chr2_241953839_R GTCTCGTGGGCTCGGAGCGGAGACCTCAGGACAG 60.6 0.07
mm4__chr8 143.29 Mbp_chr8_143289557_F TCGTCGGCAGCGTCCTGGCCCTGCGGTCTGAG 62.4 0.07
mm4__chr8 143.29 Mbp_chr8_143289557_R GTCTCGTGGGCTCGGAACTTCCGGCACTGAACCTC 60.2 0.07
mm4__chr12 46.27 Mbp_chr12_46269594_F Primer3: not found at this Tm N.d. 0.06
mm4__chr12 46.27 Mbp_chr12_46269594_R Primer3: not found at this Tm N.d. 0.06
mm4__chr2 70.79 Mbp_chr2_70790685_F TCGTCGGCAGCGTCCTGAGGCCGGGCTTTCAC 60.7 0.06
mm4__chr2 70.79 Mbp_chr2_70790685_R GTCTCGTGGGCTCGGGGAGGCCGGGATTGGAATAC 60.2 0.06
mm4__chr14_GL000194v1_random 58.71 Kbp_chr14_GL000194v1_random_58710_F TCGTCGGCAGCGTCAGCAGTTCTGGAACCACACC 60.1 0.06
mm4__chr14_GL000194v1_random 58.71 Kbp_chr14_GL000194v1_random_58710_R GTCTCGTGGGCTCGGGAGCTGCCCTGCAAAGAGTA 60.0 0.06
mm4__chr1 39.86 Mbp_chr1_39861633_F Primer3: not found at this Tm N.d. 0.06
mm4__chr1 39.86 Mbp_chr1_39861633_R Primer3: not found at this Tm N.d. 0.06
mm4__chr11 71.25 Mbp_chr11_71252853_F Primer3: not found at this Tm N.d. 0.06
mm4__chr11 71.25 Mbp_chr11_71252853_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 157.43 Mbp_chr7_157428888_F TCGTCGGCAGCGTCCCTCTGGAGGGACCTGGATT 60.3 0.06
mm4__chr7 157.43 Mbp_chr7_157428888_R GTCTCGTGGGCTCGGGTGGGCAGATTTCCCAGTGA 59.9 0.06
mm4__chr10 116.77 Mbp_chr10_116774827_F TCGTCGGCAGCGTCCAGTCCCATGCCTGCCCA 62.0 0.06
mm4__chr10 116.77 Mbp_chr10_116774827_R GTCTCGTGGGCTCGGTATAGCCAACACGAGGGGCT 60.6 0.06
mm4__chr20 63.64 Mbp_chr20_63642014_F Primer3: not found at this Tm N.d. 0.06
mm4__chr20 63.64 Mbp_chr20_63642014_R Primer3: not found at this Tm N.d. 0.06
mm4__chr20 63.64 Mbp_chr20_63641889_F Primer3: not found at this Tm N.d. 0.06
mm4__chr20 63.64 Mbp_chr20_63641889_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 131.11 Mbp_chr7_131106018_F Primer3: not found at this Tm N.d. 0.06
mm4__chr7 131.11 Mbp_chr7_131106018_R Primer3: not found at this Tm N.d. 0.06
mm4__chr6 22.57 Mbp_chr6_22570102_F Primer3: not found at this Tm N.d. 0.06
mm4__chr6 22.57 Mbp_chr6_22570102_R Primer3: not found at this Tm N.d. 0.06
mm4__chr8 141.93 Mbp_chr8_141925408_F TCGTCGGCAGCGTCTGTGTCTGGATCCTTCCCCT 59.8 0.06
mm4__chr8 141.93 Mbp_chr8_141925408_R GTCTCGTGGGCTCGGCAGCCATGGTGAGGACAGG 60.0 0.06
mm4__chr22 46.74 Mbp_chr22_46737957_F Primer3: not found at this Tm N.d. 0.06
mm4__chr22 46.74 Mbp_chr22_46737957_R Primer3: not found at this Tm N.d. 0.06
mm4__chr12 121.30 Mbp_chr12_121296814_F Primer3: not found at this Tm N.d. 0.06
mm4__chr12 121.30 Mbp_chr12_121296814_R Primer3: not found at this Tm N.d. 0.06
mm4__chr22 49.93 Mbp_chr22_49934848_F TCGTCGGCAGCGTCCTGCTCTGCACCAGTGAGG 60.3 0.06
mm4__chr22 49.93 Mbp_chr22_49934848_R GTCTCGTGGGCTCGGTGGGAACGGTCTCTCCAGAA 60.1 0.06
mm4__chr2 232.49 Mbp_chr2_232487664_F TCGTCGGCAGCGTCAGAGGACGCAGACAAAGCC 60.0 0.06
mm4__chr2 232.49 Mbp_chr2_232487664_R GTCTCGTGGGCTCGGGTAAGTGGCGCCCGAGTC 60.5 0.06
mm4__chr1 174.16 Mbp_chr1_174159469_F Primer3: not found at this Tm N.d. 0.05
mm4__chr1 174.16 Mbp_chr1_174159469_R Primer3: not found at this Tm N.d. 0.05
mm4__chr12 110.43 Mbp_chr12_110429865_F Primer3: not found at this Tm N.d. 0.05
mm4__chr12 110.43 Mbp_chr12_110429865_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 3.42 Mbp_chr1_3416083_F TCGTCGGCAGCGTCTCAGGATGAGAGCATGCCTG 59.5 0.05
mm4__chr1 3.42 Mbp_chr1_3416083_R GTCTCGTGGGCTCGGAGAAACTCGTGTCCGTCCAC 59.9 0.05
mm4__chr20 62.49 Mbp_chr20_62487805_F TCGTCGGCAGCGTCGCCTTATCTGGCCGCCTAAT 59.9 0.05
mm4__chr20 62.49 Mbp_chr20_62487805_R GTCTCGTGGGCTCGGTTCACTCCACCTCCCTCTCC 60.2 0.05
mm4__chr6 36.20 Mbp_chr6_36196828_F Primer3: not found at this Tm N.d. 0.05
mm4__chr6 36.20 Mbp_chr6_36196828_R Primer3: not found at this Tm N.d. 0.05
mm4__chr9 136.36 Mbp_chr9_136361603_F Primer3: not found at this Tm N.d. 0.05
mm4__chr9 136.36 Mbp_chr9_136361603_R Primer3: not found at this Tm N.d. 0.05
mm4__chrY 11.16 Mbp_chrY_11158919_F TCGTCGGCAGCGTCCCTGGTGGTAGCGAGCATAG 59.9 0.05
mm4__chrY 11.16 Mbp_chrY_11158919_R GTCTCGTGGGCTCGGGAGCTGCCCTGCAAAGAGTA 60.0 0.05
mm4__chrUn_GL000218v1 43.91 Kbp_chrUn_GL000218v1_43905_F TCGTCGGCAGCGTCCCTGGTGGTAGCGAGCATAG 59.9 0.05
mm4__chrUn_GL000218v1 43.91 Kbp_chrUn_GL000218v1_43905_R GTCTCGTGGGCTCGGGAGCTGCCCTGCAAAGAGTA 60.0 0.05
mm4__chr21 9.07 Mbp_chr21_9073477_F TCGTCGGCAGCGTCCCTGGTGGTAGCGAGCATAG 59.9 0.05
mm4__chr21 9.07 Mbp_chr21_9073477_R GTCTCGTGGGCTCGGGAGCTGCCCTGCAAAGAGTA 60.0 0.05
mm4__chr2 94.87 Mbp_chr2_94871655_F TCGTCGGCAGCGTCGAGCTGCCCTGCAAAGAGTA 60.0 0.05
mm4__chr2 94.87 Mbp_chr2_94871655_R GTCTCGTGGGCTCGGCCTGGTGGTAGCGAGCATAG 59.9 0.05
mm4__chr4 164.60 Mbp_chr4_164596440_F TCGTCGGCAGCGTCCTCTGTGAGTTGGGAGTGGG 59.6 0.05
mm4__chr4 164.60 Mbp_chr4_164596440_R GTCTCGTGGGCTCGGCCGCCTTTATTGGGTCTGGT 60.0 0.05
mm4__chr8 13.10 Mbp_chr8_13099785_F TCGTCGGCAGCGTCTGGAGCTGGAAGAATTGCGT 59.9 0.05
mm4__chr8 13.10 Mbp_chr8_13099785_R GTCTCGTGGGCTCGGAACGGCTCTGTGAACTGGAG 59.9 0.05
mm4__chr20 37.45 Mbp_chr20_37446619_F Primer3: not found at this Tm N.d. 0.04
mm4__chr20 37.45 Mbp_chr20_37446619_R Primer3: not found at this Tm N.d. 0.04
mm4__chr9 128.39 Mbp_chr9_128393203_F TCGTCGGCAGCGTCCTTTCCTGTGGCTGAGTCGC 61.2 0.04
mm4__chr9 128.39 Mbp_chr9_128393203_R GTCTCGTGGGCTCGGGGCGCATTCCAGCACTCC 61.5 0.04
mm4__chr6 148.78 Mbp_chr6_148778572_F TCGTCGGCAGCGTCTTGTGAATGGAGCATGGCCT 59.9 0.04
mm4__chr6 148.78 Mbp_chr6_148778572_R GTCTCGTGGGCTCGGCCCACACTGATGCAGGGATT 60.0 0.04
mm4__chr19 4.72 Mbp_chr19_4723760_F TCGTCGGCAGCGTCCTCCATGCCCGGGACTGC 62.8 0.04
mm4__chr19 4.72 Mbp_chr19_4723760_R GTCTCGTGGGCTCGGAACTTCCGGGTCAAAGGTGC 60.8 0.04
mm4__chr10 124.62 Mbp_chr10_124616660_F TCGTCGGCAGCGTCGGAATGACCTCCTTCACCCC 59.7 0.04
mm4__chr10 124.62 Mbp_chr10_124616660_R GTCTCGTGGGCTCGGGTTCCACACCGTCTCTGCC 60.6 0.04
mm4__chr17 28.52 Mbp_chr17_28524633_F TCGTCGGCAGCGTCGAGCAAGTCCAGGGCCAC 60.3 0.04
mm4__chr17 28.52 Mbp_chr17_28524633_R GTCTCGTGGGCTCGGCCTCAAAGCCAAAGCCAGGA 60.5 0.04
mm4__chr19 45.39 Mbp_chr19_45386011_F TCGTCGGCAGCGTCGGGTCGTTCATCTGAGTGCA 60.0 0.04
mm4__chr19 45.39 Mbp_chr19_45386011_R GTCTCGTGGGCTCGGGTGAAGGAGGTGAGCGCTG 60.7 0.04
mm4__chr16 73.06 Mbp_chr16_73057984_F TCGTCGGCAGCGTCGGTGGGGCAAAGTTGGGG 60.9 0.04
mm4__chr16 73.06 Mbp_chr16_73057984_R GTCTCGTGGGCTCGGGGTACCAGCTCGGAGACAAA 59.3 0.04
mm2__chr20 50.04 Mbp_chr20_50040347_F TCGTCGGCAGCGTCGGGCCTGAGACTCCGTAAAC 60.1 0.04
mm2__chr20 50.04 Mbp_chr20_50040347_R GTCTCGTGGGCTCGGCCTTCCCAGGATCTCCCACA 60.6 0.04
mm4__chrX 137.44 Mbp_chrX_137440968_F Primer3: not found at this Tm N.d. 0.03
mm4__chrX 137.44 Mbp_chrX_137440968_R Primer3: not found at this Tm N.d. 0.03
mm4__chr2 130.31 Mbp_chr2_130314343_F TCGTCGGCAGCGTCGACTGCTGGTGATCTGCGAA 60.3 0.03
mm4__chr2 130.31 Mbp_chr2_130314343_R GTCTCGTGGGCTCGGATGGACTACGTGGCTTGCAA 59.9 0.03
mm4__chr12 131.73 Mbp_chr12_131730749_F TCGTCGGCAGCGTCCCTCTGCAGGAACCCTAAGC 60.1 0.03
mm4__chr12 131.73 Mbp_chr12_131730749_R GTCTCGTGGGCTCGGTATTGAGGGGAGGAGAGGGC 60.1 0.03
mm4__chr21 45.43 Mbp_chr21_45432256_F TCGTCGGCAGCGTCGAAGGTCAGAGCTGGGGC 59.7 0.03
mm4__chr21 45.43 Mbp_chr21_45432256_R GTCTCGTGGGCTCGGCCTGCCACAGACTCTCACTG 60.0 0.03
mm3__chr9 127.79 Mbp_chr9_127786020_F Primer3: not found at this Tm N.d. 0.03
mm3__chr9 127.79 Mbp_chr9_127786020_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 135.17 Mbp_chr7_135170806_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 135.17 Mbp_chr7_135170806_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 156.12 Mbp_chr1_156115303_F TCGTCGGCAGCGTCCTGAAAGCGCGGTGAGTTC 59.5 0.03
mm4__chr1 156.12 Mbp_chr1_156115303_R GTCTCGTGGGCTCGGGGCAAAGTTATCGGCCTCCA 60.3 0.03
mm4__chr16_KI270728v1_random 1.56 Mbp_chr16_KI270728v1_random_1555137_F Primer3: not found at this Tm N.d. 0.03
mm4__chr16_KI270728v1_random 1.56 Mbp_chr16_KI270728v1_random_1555137_R Primer3: not found at this Tm N.d. 0.03
mm4__chr16 33.71 Mbp_chr16_33706946_F TCGTCGGCAGCGTCGTTCTCGCTACCCAGGGTTC 60.1 0.03
mm4__chr16 33.71 Mbp_chr16_33706946_R GTCTCGTGGGCTCGGTGGTGACCAGAGCACTTTGC 60.8 0.03
mm4__chrX 107.83 Mbp_chrX_107825920_F TCGTCGGCAGCGTCGGTTTCTCCGGTCACTCCTG 60.0 0.03
mm4__chrX 107.83 Mbp_chrX_107825920_R GTCTCGTGGGCTCGGCCCAAGAAGAAGGGGTGCTC 60.3 0.03
mm4__chr16 87.38 Mbp_chr16_87383317_F TCGTCGGCAGCGTCCACCCAAAACACCACATCGC 60.3 0.03
mm4__chr16 87.38 Mbp_chr16_87383317_R GTCTCGTGGGCTCGGGTCCCTGCCAGGTGGAGG 61.7 0.03
mm4__chr16 35.02 Mbp_chr16_35023241_F TCGTCGGCAGCGTCAATAGCGCTCAGGACCCAGA 60.6 0.03
mm4__chr16 35.02 Mbp_chr16_35023241_R GTCTCGTGGGCTCGGAGGAACTGCAGGCTGAAAGG 60.2 0.03
mm4__chr16 34.39 Mbp_chr16_34388563_F TCGTCGGCAGCGTCAGGAACTGCAGGCTGAAAGG 60.2 0.03
mm4__chr16 34.39 Mbp_chr16_34388563_R GTCTCGTGGGCTCGGGAATAGCGCTCAGGACCCAG 60.2 0.03
mm4__chr4 183.48 Mbp_chr4_183483481_F TCGTCGGCAGCGTCCGAATCTTGGTGGCCGCT 60.4 0.03
mm4__chr4 183.48 Mbp_chr4_183483481_R GTCTCGTGGGCTCGGGGCACCCCTTGTAGGTCTTC 60.0 0.03
mm4__chr19 7.83 Mbp_chr19_7832115_F TCGTCGGCAGCGTCGTGTCCCTGTGTCTTTCCGT 59.8 0.03
mm4__chr19 7.83 Mbp_chr19_7832115_R GTCTCGTGGGCTCGGCACAGAAGCCCAGAGGAACC 60.3 0.03
mm3__chr3 13.93 Mbp_chr3_13933044_F TCGTCGGCAGCGTCGGAGCCCTGAGTCCGGAG 61.1 0.02
mm3__chr3 13.93 Mbp_chr3_13933044_R GTCTCGTGGGCTCGGGAATCCTGGGAAGCTCGTCC 60.1 0.02
mm4__chr17 81.87 Mbp_chr17_81870835_F Primer3: not found at this Tm N.d. 0.02
mm4__chr17 81.87 Mbp_chr17_81870835_R Primer3: not found at this Tm N.d. 0.02
mm4__chrX 9.50 Mbp_chrX_9495276_F TCGTCGGCAGCGTCTTCTCTGTGATGGAGCGCAG 60.1 0.02
mm4__chrX 9.50 Mbp_chrX_9495276_R GTCTCGTGGGCTCGGCCTCTCCCTTGTGCACCATT 59.9 0.02
mm4__chr6 163.35 Mbp_chr6_163352419_F Primer3: not found at this Tm N.d. 0.02
mm4__chr6 163.35 Mbp_chr6_163352419_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 19.22 Mbp_chr17_19223313_F TCGTCGGCAGCGTCGCCCAAAATAATCGAGTGTAAGGC 60.4 0.02
mm4__chr17 19.22 Mbp_chr17_19223313_R GTCTCGTGGGCTCGGGAAAGACTTGCCACCCTGCT 60.5 0.02
mm4__chr17 19.15 Mbp_chr17_19154917_F TCGTCGGCAGCGTCGAAAGACTTGCCACCCTGCT 60.5 0.02
mm4__chr17 19.15 Mbp_chr17_19154917_R GTCTCGTGGGCTCGGTGACATCTCAGTCTTGATTTGCAC 59.5 0.02
mm4__chr17 19.10 Mbp_chr17_19097146_F TCGTCGGCAGCGTCGCCCAAAATAATCGAGTGTAAGGC 60.4 0.02
mm4__chr17 19.10 Mbp_chr17_19097146_R GTCTCGTGGGCTCGGGAAAGACTTGCCACCCTGCT 60.5 0.02
mm4__chr22 46.74 Mbp_chr22_46738217_F Primer3: not found at this Tm N.d. 0.02
mm4__chr22 46.74 Mbp_chr22_46738217_R Primer3: not found at this Tm N.d. 0.02
mm4__chr8 142.40 Mbp_chr8_142403288_F TCGTCGGCAGCGTCGACTACCCGCCCCAGAGG 61.1 0.02
mm4__chr8 142.40 Mbp_chr8_142403288_R GTCTCGTGGGCTCGGCACCAGGCAAGTGAGGACAG 60.6 0.02
mm4__chr4 6.73 Mbp_chr4_6732843_F TCGTCGGCAGCGTCGTCAGGGTGACTCAGCTGTT 59.6 0.02
mm4__chr4 6.73 Mbp_chr4_6732843_R GTCTCGTGGGCTCGGGAACTTGGTGAGTCCTCCCG 60.0 0.02
mm4__chr18 57.44 Mbp_chr18_57435827_F Primer3: not found at this Tm N.d. 0.02
mm4__chr18 57.44 Mbp_chr18_57435827_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 133.43 Mbp_chr9_133428578_F TCGTCGGCAGCGTCGAAAACTCGCTGGCGCTG 59.8 0.01
mm4__chr9 133.43 Mbp_chr9_133428578_R GTCTCGTGGGCTCGGCTCCAGGCCGAAGCTGGT 61.7 0.01
mm4__chr8 22.06 Mbp_chr8_22056747_F TCGTCGGCAGCGTCCACATCCACACACACACCCT 60.1 0.01
mm4__chr8 22.06 Mbp_chr8_22056747_R GTCTCGTGGGCTCGGTGGGGAGTCCTCGTCTGG 59.9 0.01
mm4__chr20 36.85 Mbp_chr20_36852356_F Primer3: not found at this Tm N.d. 0.01
mm4__chr20 36.85 Mbp_chr20_36852356_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 99.82 Mbp_chr9_99822140_F TCGTCGGCAGCGTCGCTCACTTTGCAACGCTGAC 60.6 0.01
mm4__chr9 99.82 Mbp_chr9_99822140_R GTCTCGTGGGCTCGGCGACTCATGGGAGAGCACAG 60.1 0.01
mm4__chrX 49.22 Mbp_chrX_49219604_F TCGTCGGCAGCGTCAGTTCTGGCCCTGACACAAC 60.1 0.01
mm4__chrX 49.22 Mbp_chrX_49219604_R GTCTCGTGGGCTCGGTTCTGCCTCAGCCAAACCAA 60.1 0.01
mm4__chr19 7.47 Mbp_chr19_7470220_F TCGTCGGCAGCGTCTCCGAGGGCTTCTCTCTCAA 59.9 0.01
mm4__chr19 7.47 Mbp_chr19_7470220_R GTCTCGTGGGCTCGGGTCTTCTTTGCTGGCGTCCT 60.6 0.01
mm4__chr2 21.35 Mbp_chr2_21345246_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 21.35 Mbp_chr2_21345246_R Primer3: not found at this Tm N.d. 0.01
mm4__chr18 7.57 Mbp_chr18_7567446_F TCGTCGGCAGCGTCGGGCTGCCTGTGGAGAGG 62.1 0.01
mm4__chr18 7.57 Mbp_chr18_7567446_R GTCTCGTGGGCTCGGCTCGGTTCCGGTTGCTGAG 60.7 0.01
mm4__chr15 80.70 Mbp_chr15_80695056_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 80.70 Mbp_chr15_80695056_R Primer3: not found at this Tm N.d. 0.01
mm4__chr6 37.73 Mbp_chr6_37727978_F Primer3: not found at this Tm N.d. 0.01
mm4__chr6 37.73 Mbp_chr6_37727978_R Primer3: not found at this Tm N.d. 0.01
mm4__chrX 75.75 Mbp_chrX_75747168_F Primer3: not found at this Tm N.d. 0.01
mm4__chrX 75.75 Mbp_chrX_75747168_R Primer3: not found at this Tm N.d. 0.01
mm4__chr21 37.07 Mbp_chr21_37073287_F Primer3: not found at this Tm N.d. 0.01
mm4__chr21 37.07 Mbp_chr21_37073287_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22 17.01 Mbp_chr22_17008016_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22 17.01 Mbp_chr22_17008016_R Primer3: not found at this Tm N.d. 0.01
mm4__chr8 64.58 Mbp_chr8_64581051_F TCGTCGGCAGCGTCTGCTTCGAGCCGGCTGAC 62.7 0.00
mm4__chr8 64.58 Mbp_chr8_64581051_R GTCTCGTGGGCTCGGCTGAACCTACTAGCAGCCCG 59.8 0.00
mm4__chrX 132.45 Mbp_chrX_132449982_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 132.45 Mbp_chrX_132449982_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 20.66 Mbp_chr1_20661515_F TCGTCGGCAGCGTCGGAAGTGATCGGGATACGCC 60.3 0.00
mm4__chr1 20.66 Mbp_chr1_20661515_R GTCTCGTGGGCTCGGGCCTTGATTCACTCCCACCA 59.9 0.00
mm4__chr10 120.25 Mbp_chr10_120252085_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 120.25 Mbp_chr10_120252085_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 93.22 Mbp_chr9_93218344_F TCGTCGGCAGCGTCCTGTGTCCCTGGTGCTATCC 59.8 0.00
mm4__chr9 93.22 Mbp_chr9_93218344_R GTCTCGTGGGCTCGGCCCTGCATCTGACCACATCA 59.7 0.00
mm4__chr16 79.56 Mbp_chr16_79564090_F TCGTCGGCAGCGTCGAGACCTTTCAGGCAGGTGG 60.3 0.00
mm4__chr16 79.56 Mbp_chr16_79564090_R GTCTCGTGGGCTCGGTAGCAGAAATCCCAGGAGGC 59.1 0.00
mm4__chr6 165.86 Mbp_chr6_165855293_F TCGTCGGCAGCGTCAATGAACAGAGGCGGATGGG 60.1 0.00
mm4__chr6 165.86 Mbp_chr6_165855293_R GTCTCGTGGGCTCGGGCTACCAGGTGATCCACAGG 59.8 0.00
mm4__chr8 143.29 Mbp_chr8_143291347_F TCGTCGGCAGCGTCGGCAACCGATGAGCTGAAGG 61.3 0.00
mm4__chr8 143.29 Mbp_chr8_143291347_R GTCTCGTGGGCTCGGGAAACGCCAGTCTCCACCC 60.6 0.00
mm4__chrX 140.09 Mbp_chrX_140091409_F TCGTCGGCAGCGTCGTAGGAGCGGACCGTGGG 61.8 0.00
mm4__chrX 140.09 Mbp_chrX_140091409_R GTCTCGTGGGCTCGGGGAGATAACCCCGCAGCC 59.8 0.00
mm4__chr7 44.49 Mbp_chr7_44490648_F TCGTCGGCAGCGTCGTACGCTTCACACACACAGC 59.7 0.00
mm4__chr7 44.49 Mbp_chr7_44490648_R GTCTCGTGGGCTCGGGAGCACGGACCGGAAGTTC 60.7 0.00
mm4__chr3 127.28 Mbp_chr3_127284474_F TCGTCGGCAGCGTCGACTAGGTACATGCCTGGCC 59.8 0.00
mm4__chr3 127.28 Mbp_chr3_127284474_R GTCTCGTGGGCTCGGGCACAGCCAGCTCCTCCT 61.7 0.00
mm4__chr19 9.91 Mbp_chr19_9913861_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 9.91 Mbp_chr19_9913861_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 3.97 Mbp_chr19_3971050_F TCGTCGGCAGCGTCCGCAGCCCGGAGAATACG 60.6 0.00
mm4__chr19 3.97 Mbp_chr19_3971050_R GTCTCGTGGGCTCGGGGGGTTCGTTTCCCGGGG 62.7 0.00
mm4__chr18 23.45 Mbp_chr18_23453175_F TCGTCGGCAGCGTCGCTGATGCTTGCTTCTCCGA 60.7 0.00
mm4__chr18 23.45 Mbp_chr18_23453175_R GTCTCGTGGGCTCGGTTCGCTAGGACAGTCGCTTG 60.1 0.00
mm4__chr17 79.74 Mbp_chr17_79739394_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 79.74 Mbp_chr17_79739394_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 23.54 Mbp_chr16_23535202_F TCGTCGGCAGCGTCCAGGTGGTATGTGGGGAAGC 60.3 0.00
mm4__chr16 23.54 Mbp_chr16_23535202_R GTCTCGTGGGCTCGGCAGGACCTGGTCTATGGCTG 59.5 0.00
mm4__chr12 54.47 Mbp_chr12_54473592_F TCGTCGGCAGCGTCCACAGTCACCTTCCTCCCAC 59.9 0.00
mm4__chr12 54.47 Mbp_chr12_54473592_R GTCTCGTGGGCTCGGCTCAGCCAGAACCGAGACTC 59.8 0.00
mm4__chr10 31.32 Mbp_chr10_31318844_F TCGTCGGCAGCGTCAATCAGCCAGATCCCTCCCT 60.0 0.00
mm4__chr10 31.32 Mbp_chr10_31318844_R GTCTCGTGGGCTCGGGCTGTCGGAGTTGGAAAGGT 60.2 0.00
mm4__chr10 122.05 Mbp_chr10_122051027_F TCGTCGGCAGCGTCGAGGCTGGTGGATGAATCCC 60.1 0.00
mm4__chr10 122.05 Mbp_chr10_122051027_R GTCTCGTGGGCTCGGGGAAGTGACTGATGGGCCA 59.3 0.00
mm4__chr1 12.47 Mbp_chr1_12473388_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 12.47 Mbp_chr1_12473388_R Primer3: not found at this Tm N.d. 0.00
mm3__chr7 27.10 Mbp_chr7_27097013_F TCGTCGGCAGCGTCCTACTCGACCGCCGGATTTT 60.1 0.00
mm3__chr7 27.10 Mbp_chr7_27097013_R GTCTCGTGGGCTCGGGGAGGCTCCTGGGTCTCA 59.9 0.00
mm3__chr5 443.16 Kbp_chr5_443161_F Primer3: not found at this Tm N.d. 0.00
mm3__chr5 443.16 Kbp_chr5_443161_R Primer3: not found at this Tm N.d. 0.00
mm3__chr20 37.41 Mbp_chr20_37406611_F TCGTCGGCAGCGTCGTGGATGTGGGTGGGAACAG 60.6 0.00
mm3__chr20 37.41 Mbp_chr20_37406611_R GTCTCGTGGGCTCGGCTTTGTCCCGGTTGCGCTC 61.9 0.00
mm3__chr20 32.99 Mbp_chr20_32985581_F TCGTCGGCAGCGTCATCCCCTACCCTTGAAGGCT 59.9 0.00
mm3__chr20 32.99 Mbp_chr20_32985581_R GTCTCGTGGGCTCGGGTCTGCACAACACTTGCAGG 59.9 0.00
mm3__chr11 809.53 Kbp_chr11_809531_F TCGTCGGCAGCGTCAACCACCAAGGAGTCAAGGC 60.1 0.00
mm3__chr11 809.53 Kbp_chr11_809531_R GTCTCGTGGGCTCGGCTCAACCTTTGCCAGCGAAC 60.0 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529700 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm2__chr20 63.89 Mbp_chr20_63890192 CTGTGCTGTCCTTCCTAGGGGTGCAGGAAGTGGACAGGGCGGAGGGTTTGAAAGAATATTGAGCCAAAGCCCAGGCTCCC
TTTGGGAATCATGTTAGCCCATCAGAATGTTGAAGGA
mm4__chr15 68.58 Mbp_chr15_68580604 GGGACTTGGGAGCATTGTGAGAAGATGAACTTGAGGGTGCAGGGGCTTCCTCAGCTCTGGCTACTTCTGGCAGGGCTGTT
GTGGCCCACCTGGGGTAAGATGCTT
mm3__chr12 132.75 Mbp_chr12_132745993 CTGTGCTGTCCTTCCTAGGGGTACAGGAAGTGGACAGGGCGGAGGATTTGAAATACTGAGCCAAAGCCCAGGCTCCCTTT
AAGAA
mm4__chr19 58.02 Mbp_chr19_58020285 GATGGGAACTGGGTCCTGTCGCTGAGGCTGGCCAGTGTGGAGGAGGGGCTGACCTGTGCAGGAAGAGGCCAGGGCAATGG
GGAGGGGTGAGGAGCAGCTCAGGCACCTGCTGACCACCAGGTTCAAAAGAAAGCCAGG
mm4__chr17 77.36 Mbp_chr17_77364602 AGAGCGGAGAGGTTCCTCTTCTCTCTTCTCCATCATCCTGTGGAGGTGTCAACATGTAGCCAGAGATGGTCAGTGGCAGT
GAGGAGAGGGGGGCAGGAAGAGACCAGGGCTGGGGGCTTGCCAGCCCACAGTTCTA
mm4__chr8 10.33 Mbp_chr8_10334856 TGTGCCTTACTCCCTCTGGTGGGACCATTTAACTCCAGCGCGCCACCCCTCCCCCGCTCTGGCCGCTCCCGTCTGCCCCG
CTGCCTCGGCCCACGTGATGGCTGTTCGGGCCCCGCCGAGCCATCTTCCCGTTGCTA
mm3__chr14 75.47 Mbp_chr14_75470354 AGTTACGGCCGTTGTGTGTAACTCCTAAGTACTGTAGTCTCTGGGTGTCGGGGGTGGCCAGGGCGGGGGCGGGGTGCATT
TCCATCCTT
mm4__chr16 10.84 Mbp_chr16_10835454 ATAACAGACCAGCCAGCCACACCGCTGCCCTGGCCACTTCTAGAGGTCCAAGTCTCTTGAGATCCCCACTGCCTTGTGGA
GAGGGCCATGAATCCTG
mm4__chr3 98.52 Mbp_chr3_98522622 CCACCTCCTGGGCCTCACGGCGGGGCCGGAGAAGGCCAGGGCTGGGGCGGGGCAGCCGTGCTCGGCCCCGGGCCAGGGTC
CCCCGGTACCCGACCAGGCCCCGCCCTAGAGGGCTCGGCCCTGGGACAAATC
mm4__chr3 129.02 Mbp_chr3_129022442 ACATGAAGATCTGGAGCCAGGAGCCAGGAGTGGCCAGGACAGCGGAAGGGAGGAAGAGCAACATCTGGCC
mm4__chr5 170.18 Mbp_chr5_170183106 CACTCTCAGCCCCTTTCCTGCCAAAATGTTCAAGGTACAAGAGCCATGTGGACTCTGGTCTGAACAGGGGGGCTGAGCCA
TCTCTGGAAGAGGCCAGGGTGGAGGGGAAAACAGAAGGCTTAGCTCTTCTGTCTAACCA
mm4__chr15 77.03 Mbp_chr15_77034245 CAGCACAATGGCTCAGCAAGCCCTGCACCCCTTTCCCCCTGCCCTGGCCGCTTCCTCCACCTGTCCCCAGCCCCTGCTGG
CTTGCACCCTGTCCTTTGCT
mm4__chr22 43.22 Mbp_chr22_43223240 GCGAGAAAGACACTTGCCTGTCTCTATGAAGGGAGATACGAGAGAGGGCTGAGAGAGGCCAGGGCGGAGGCTTCCTGGAG
CCAGGAGGGTCCTGGGTATGGCCTAATGGGGA
mm4__chr6 40.89 Mbp_chr6_40891281 CCAGGAGGAACAAGAGCAGGCTTCAAGGGGCAGGGTGGAGCCACCCCCTGGCCTGTCTGCACAAGTGTCCCAGCTCCCCA
CGTCCTGGCCACTTCCTCCCACTGAGATGTGCTTCATGC
mm4__chrX 143.63 Mbp_chrX_143634511 CCACCCGAGACCTGTACAACTTGCCCCGCCGCCCTGGCCGCTTGGGGTATGGTGGGGGGGGGGGCGGGCAGCGATGCCAA
GGACACCCGGATCCTTT
mm4__chr16 14.94 Mbp_chr16_14938171 CCTGCCCTAAGCCACCTCCACCTCTGTCCTGGCCACCTCCGGGTGCCCTGAAAGGACCAGGACATGCG
mm3__chr8 5.73 Mbp_chr8_5734064 TGGAACCAGCCGAAGATTCCTTCTCTGGCTCCAGTCGTGGGTGGAAGAGTTCACCGGTAACAGCCTGGCACGCAGCACGA
AGTGGCCAGGGCAGCGGGTGCCTGCAGTATGGTGGGTAG
mm4__chr2 1.50 Mbp_chr2_1503596 TCCTTGCTCTCAGAGGACCTGCCGGCCAGCTGGGAGCATCTGCCTGGGCGAAGCAGACCCTGCCCCACCCTCACCCTGGC
CCCTTCCTGCCCCCGTGTCCTGCACTTGGCCAAATTCC
mm4__chr4 1.10 Mbp_chr4_1097012 ACAGCCTCGATCAACAGGACAGCCCCTCGTCCTGGCCACTTCCCTCTGAGGGCTGTGCCGGACAGTGGTGAGGGCCAGGA
GGCAGAGCAGCCTGCCTGGCAAGGACACGCTCCCTCCTCAAAA
mm3__chr8 144.42 Mbp_chr8_144416551 GGTACTGCCACTAGCCTCCTCCGCCTCCTGGAGTTTGCCCAGGGCCCTTTGCTGCCGGCTGGCAGGGCGGAGCTGGCGGG
AAGAGGCCAGGGCTGGGGGACCCGTCGGGTGGGGCTGTTACCTTTCCA
mm4__chr5 178.72 Mbp_chr5_178718368 CCCAGTCCTCAGCTACCTGTTAAACAAGGTGAAGACCTAGGTCAGAAGTTGAGAAGCCAGAATCATCTTGCTGATGGCCA
CTGCCCTGACCACTTCCTGTTATCAGGTCTAGACTCTTCCACCTGGACTCTCACTACA
mm4__chr1 19.37 Mbp_chr1_19370136 CCAAGGCTCCCAGAATGACACCCAGAGCCCGCACCCCACAGGCCTGTGCGCCTCTCCCCACTGCCCTGTCCTGTTCCGGC
CTCCACTCACTGCCCACGACCACTGGGAATCC
mm4__chr2 44.95 Mbp_chr2_44949885 CTGGAGCTGAGATGCCCGGCCCGCGGGACGCTCGACCCCCTGGTACGCGCGCTGAGGCCCCCACGGCTTCCCACCGCTCC
GGCCCTTTCCGGCCCCAGCTGAGCTGAAGCTCCCTGGCCCCAG
mm4__chr7 44.23 Mbp_chr7_44226541 GGCCACTCAAGGTGCTACTTACATGGGGTGGGCAGGGGGCCAGGGATAGTCGGAGATGGGCAGGGCGGGGGCCCCACTGG
CGAGGGGCCCTCGGCTTCTGGGGTCCCTGAGCCCCTCCTCACAGAGTTCAG
mm4__chr12 124.57 Mbp_chr12_124565212 AGTATTGATCCCCAGCAGCGTAAATAGCTCAGTGGGAAGAGGCCAGGGCTGGGGCCTGCTGCGCTCCCGCCCCCATGTCT
GGGCACCGGCACCACAGACTCCCCCGGCACCCGCTGGCGGTGGCAGCAGTGAATTTC
mm4__chr4 7.53 Mbp_chr4_7530985 CGAACTCTGGTACCTGCCTGCGGTCTCCTACCATCCTGTGCTGCAGGTGGTGGCCAGGGTGGAGGATGGGGCTCCCTGGA
GGAGCTCAGAGTCCAGAGAGGG
mm4__chr1 21.90 Mbp_chr1_21904662 ATTAGGTCTCCCCTGGTGGCTGAATGAGCTGCCTGCCAGCTTACAGGGAACCAGGAAGCCACCGGGAAATGCCCTTGCCT
CACCCATGTGCCAGGTGTGGCCAAGGCGGTGGGGGTGGCCTAAGCGGGAACAGCTTCTGG
mm4__chr11 76.65 Mbp_chr11_76653183 AGGGCAGTCAAGTCACCAAGTCGCAGGGAGGGGCCAGGGCTGGGGGGCTGCTTTCAGTGCATGAGAAAGGACCTCAGGGT
CCAGAGTTCAGGCCCCATTCTCC
mm4__chr10 85.79 Mbp_chr10_85787238 CAGTCCTGGAAGGGTTTGGTAAATGTGCCTGCCCCTGCCCTGGCCACTCCCAGGTATCCAGTTGGGCCTACTGGTGCTGA
CATCCTCATTGCCTGGATCTGAACACTCTGTCCTCAGGTCCATCTTGGCCATGCACTC
mm4__chr22 23.39 Mbp_chr22_23394377 GGCATCTGAAAAGTGGCTGCAGGAGCCTGTCCAGGAGGAAGGGGCCAGGGCTGCGGTGCGGGGAGCCCTGTCTGAGGAGG
GTTTCC
mm4__chr22 20.15 Mbp_chr22_20149023 GAAACCCTCCTCAGACAGGGCTCCCCGCACCGCAGCCCTGGCCCCTTCCTCCTGGACAGGCTCCTGCAGCCACTTTTCCG
ATG
mm3__chr22 38.14 Mbp_chr22_38143532 CTCGTACAAGAGATGGGCCCCAGGGCCACACCAGCAGGAAGTGGGCAGGGTGGTGGCACAGGAAAGGGTCACAGAACACG
GA
mm4__chr20 62.35 Mbp_chr20_62353610 TGCAGATGGAGCAAGCTAGGGAGAGGCCTACCTGCGGCCCAGGCAGGGACTGGCCAGGGTGGAGGCAGGAACACACCCCA
GTGTCCCCACAGCCCTCACGTCCAGCCAGGCCTGGTGGCCAGACAGTGGTC
mm4__chr10 29.74 Mbp_chr10_29735271 CCACGCATCGGGTTCAAACCCGCGCGGGCCCTGCGGAGGCGCCGGTCTGGGACCCCGGGCTGGGGCGCGGGAGCCACCGC
AGCTCCGGCCACTTGCGGCTGGTGTCGGGAAAGGAACCGGGCGATGTCGTAGTTCTGA
mm4__chr21 42.25 Mbp_chr21_42253200 AAGGCTGTTCCCAGGGATTGCACCTGCCTGGCCCTGCATCCAGGGAGGATCCTTTCAGCTGGAGATGCCGGAAGTTGGCA
GAGCTGGGGTAAAGGGCAGAAACATAAAGACCAGGTCAGCAGCAGCTCCAACTAAGGCAG
mm4__chr16 53.36 Mbp_chr16_53358817 AAGGGCCCTAGTCCAGTTTGCTGTAGAAAGACCAGTCTTCCACTGTATGGCACATGGATGGCAGGGGCAGAGTGCAGGTG
GGGAGAGCAGAAGGTGGGCAGGGCGGAGGAGGCAGGGACATGGCTGTAG
mm4__chr2 235.55 Mbp_chr2_235551137 TCTGAGGAAGAGAAGTGGGGGGTCGGGGGCATCCCACCTCCCTGGCCACCTCCAGTGGTGTGAGTTGGTTGCTTGATGTC
ATCTGAGGTGGGGCCACCCGGGAAAGGTCTTCAT
mm3__chr9 34.62 Mbp_chr9_34621247 GGGTTACTGGAGGAAGAACCCTTTGGTAGGTGCTGCTGGAATTGGCCAGGGAGGAGGTGCCCTCCCTTTACAATGAGGTA
GGTGTAACATCAAAAGAAACTTTGGTTTTAAGTCCACATGGCATGGGAG
mm4__chr7 44.23 Mbp_chr7_44229413 AGCAGGACCCAGGCTACTTACATGGGGCTGGCAGGGGGCTAAAGGGAGCCGGAGATGGGCAGGGCAGGGGCCCCTCGGCT
TCTGGGGCTCCCGAGCCCCTCCTCACTGAGCTCAGGATGTCGG
mm4__chr6 25.63 Mbp_chr6_25627802 ACCTGCAGAGCCTCATGTTCAGATGGAAATTGGTCTTTTGAGCATCTTCCCAGCACCCTGGCCTCTTCCTGCCCTCCACC
ATACCACTTACCTCCTGAGGGTGGAAAAGAAGCTCAGC
mm4__chr10 116.86 Mbp_chr10_116856544 TCTGTGGTGATCTCGACTCATTTTGAAGTCCATGAGAATGCTCTGCCCGAGCTGGCCAAGGCGGAGGAGGCAGAGAGGGC
CAGCGCGGTGAGCACCGCCGTGCAGTGGGTCAACAGCACCATC
mm4__chr19 7.91 Mbp_chr19_7908206 GTGGGAGGTGACGTGGTGCTGCAGAGGCGGCAGTGGCCAGGACAGCGGGAGGGGCACGGGAGGTGAGGCCTCTAGGCCAG
TCATGAACGCTG
mm4__chr6 138.46 Mbp_chr6_138464250 CTGACAGAGGCTGAGAGGCTGCTGGCCGCCAGCTTGGCCATCACGGCCGGCACCGTCCTCCTCTCTGCCCACTTCCTGCT
TTGGCAGACCCTGGTGTC
mm4__chr2 8.59 Mbp_chr2_8593932 CTCCAACGTGACAAGAGGGGCTCCCATGTCCGCTGACCTGTGCAGTTAAACAACCTTGATAGGTGTTTCCTTTTCCTCCT
CCCTGCCTTCTTCCGGCGTGCTCTCCCAGCCCATCCCTGGTGATCCTCATGCCTC
mm4__chr5 133.79 Mbp_chr5_133789168 CACATGCTAACCTGAGCCCAGTGATTCAAATGGAGCCAGGAGTGGGAGGTGGCCAGGGTGGTGGGGGAAGGTTGGCCATG
TTACCAATTCATTCATGTTCTAAATCTGCATCCGAAGGGAACCTCACC
mm4__chr8 81.62 Mbp_chr8_81621315 ATTGGCCACTGTGCCTTACAGTAAGGCCAAACTAACCTCTGCCCCGGCCACTTCCAGTGCTTCCCTCTCCATGACCAT
mm4__chr3 122.68 Mbp_chr3_122681181 CCTCCAGGGAAATGGCGGCAGGGCACGCACGGGAGGGTGACCCGCCCGACTTCGGCGGCTGCTGTAGCGGAGGTGGCCNN
NNNNNNNNNNNNNNNNNNNNNNNCAGAATGGATTCCGAGCGCA
mm4__chr14 101.15 Mbp_chr14_101151541 GTGGGGCAGAGTGAGTCATGGCAGCGCCACCCACCTCGCCGGGTGAGGCCAGGGCGTGGGCTCTTGCCTGAACACTCCAG
GGCTTGCTTC
mm4__chr17 76.16 Mbp_chr17_76156377 GGGACACAACAGGACATGGAGCAAGCGCCAGTCACCTGTGCTGGAGGAAGGGGTCAGGGCGGAGGTCTGGGTAGAGATGA
GGAGGAAAGGCGACAC
mm4__chr8 143.04 Mbp_chr8_143042968 GCCCAGAGGACAAGGAGTTTAAGCAGGGATATGTTCCCTGACAGGGAAGCCCCGACCTCTGCCCTGGCCACATCCTTCCC
CTGATCTCATGGCTGAAAAGCCACACCAGAATTCCTGCAATTCAGTGAGGCAGCT
mm4__chr2 238.80 Mbp_chr2_238796505 CTGCAGGTCCCCTTCATGTCCGTGCCTGCCTCTCTGTCCCTGGACCACCCCTGCCCTGCCCACTCCCTGCTCCACATCAG
CTTCTCACTAAGTCCTGGTTCCCTCCCATCCTCCTGTAACCTTTCCCTTCTGTGCCC
mm3__chr15 90.20 Mbp_chr15_90201079 AAACCAACGCCACTAGCAGAGGGTCCCAGAGGAGCCGGAAGGGGCCAGGGCCTGGGCGGCGGTGGGAGGGTCTGAGCAGT
G
mm4__chr18 79.34 Mbp_chr18_79337799 ATGCCATTTCCCAGGGATGGTTATGCACCCCCCACAGGACGCATGGAGCCAGTGCCCGTCACCCTGGCCACCGCCGGCCA
GATGTCTGCAGCCATTCCT
mm4__chr6 31.93 Mbp_chr6_31933899 CTGACTCCTGTGTGGCTCTCCCCACAGAACCCTACTCTTATGACTTCCCTGAGGACGTGGCCCCTGCCCTGGGCACTTCC
TTCTCCCACATGCTTGGGGCCACCAATCCCACCCAGAAGACAAAGGG
mm4__chr20 3.82 Mbp_chr20_3824125 GAACCTGCTACTGGCTGAGGGCACGCTCCGGCTGCTGACACGCCTCCTCCTTGACCACCTCCGGCTGCTGGCGCCCAGCA
CCAGCCTTCTGCTGCGGGCTGACCGCATTGAGGGCATCCTCACCCGCTTCCTG
mm4__chr21 43.95 Mbp_chr21_43947158 TCATCCTCCCCTCGTCCTGCCCAGCTCCACCTCCTCCCTGCCCTCTTCCTGCCAGGGTTTGCAGTTTACACTGGCAGTGA
TGCAGTGGCAGACACAACTTG
mm3__chr4 148.50 Mbp_chr4_148504570 AGGGCTACATGATGAGGGGAACCAGAAGTGGCCAGGCCGGAGGAAGGAGCCACACTGTTGGCTAAATCTGAGAGGGAGGT
AAGCCAGTGGTCTG
mm4__chr14 74.33 Mbp_chr14_74328207 AGTACCTCCAACTTCCTGGGTTATGAGCCAATATATCAAAAGGTACCCTGCAGGGTGAGGACGGAAGACCCTGTGCCCTG
GCCACCTCCTGCCTCAGAGAAGCCACAATAGAGCTGTCAGAGGTGGCCATGGTTTC
mm4__chr12 110.37 Mbp_chr12_110370605 CTGTGCTGCCAATGACCTTCCACAGCAATCTGAGAAAACCCTACACTCTGGACCAGGCTGGTGGTGGCCTGGGCGGGGGT
GCTGTTTGCCCAGGCCTGGAGGGGTCTAGACGGCAGA
mm4__chr16 29.98 Mbp_chr16_29977251 AGAGTGACTTTGCGGGCTCCTCTGGGCCAGTACCCACTTCTAACAATGGACGGATTTCCACCGCCTCCGGCCTGGCCATT
TCCTGTTCTCTTCCAGGGTGTGAGAGGTTGAGGGAGGAGG
mm4__chr16 88.73 Mbp_chr16_88726526 TCCATCATCTCCTTGGCTGCAAGGCAGGCACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCTTCCCCACGCCC
TCCCCCGCCACCGTCCTGGCCACTCACGGTCATAGTAGCCCTTGACGGTGCATA
mm4__chr11 61.74 Mbp_chr11_61739689 CAGAGCAGTGCTGGTAGGTTCTGCCAGGGTCTGCTGCTGCAGGGGGTAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNCCTTGGCTCAATCACCGCTCGGGGCTGGGGGTGGAGGCTGGGAGAAGATG
mm4__chr10 16.44 Mbp_chr10_16437006 AGGGCACAGGGGAGACTAAAAAGGCCAGGGAAGTTGGCCTGCCGGAAGTTCCCAGGGAGAAGGCAGCGCGTTGTACCCGA
GGTAAGGCTTCTTGG
mm4__chr1 111.90 Mbp_chr1_111904921 AGACTCAGGATGCACTTGGGCCACCACCCTCGGGGGCTCCCAGCTGGCCAAGCAGGGATCACGTCCCTCTGCCTTGGCCA
CTCCAGGCCACCTGCAGAGGGGGCAGCCCTGTCAGTGATGGAG
mm4__chr22 39.39 Mbp_chr22_39388829 GCTGTCATCCCTTAGTGGCCACGCCCGCCTCCCTGTCCACTTCCCGTCTTCGTCGTTGCGGTTCAGGTGTTCGCTCCACT
C
mm4__chr1 115.68 Mbp_chr1_115679385 GGAGGACTGTGAGAGCCATGCCTGTGACACCCAGGGCTGGCATCCCACAGTACCCGCCCCCACCCTGGGCACTTCCTGGG
ACGCCAGGACTTTGCTCTGAGGGGCTGTCAGGTGGGCTGTGGACTTGGCTTAT
mm4__chr2 28.64 Mbp_chr2_28643113 ATAGCCACTCTCTTCACCGCCCTCTGCCCCAGCCACTCCCGGCCACCAGGACATGCTTCAATGCCTGGTGCCATAGGAAG
CCCAGGGGACAGTCACAACTTCTTGGGGCC
mm4__chr16 2.34 Mbp_chr16_2341664 GAGGTGGTCCGGAGAGGGTCGGGGTCACCTCACGGAGCGAGCGGAACGGGGTCACCTGGAGGGAGTGTCCAGGGCGGGGG
CCCTGCAGGGAGTCGCCTGCAGGGAGGGTCCCGGGCGTGTCACATTAGGCGGTCCCAG
mm4__chr3 136.63 Mbp_chr3_136630366 GTAGCCGGAACACCTCAACAATCTCAATCTAAGGAAAAAGAAAGAATCATAATAAAAGAGCTGGAAGTGAGCAGGGAGGG
GGGGTAGAGAAGGGGTAGAGAAAGAGTATGGT
mm4__chr10 45.37 Mbp_chr10_45374488 CTGAGGTGCGTCCGGGACCCGGTTTGGACGGCAGAGGCCTGGGCGGGGGCGCCGAGGGCCCGTCGGGGCGGCCCGGACAG
GACTGGGGGTGTCCAGGACCCTGTCAGGGAGGGCAGAACT
mm4__chr7 332.54 Kbp_chr7_332543 GTCCCGTGGATGCTGACAGCCCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNCCTCCCACCGGGCGCCGGGGTCTCCCTGGTCCACACACC
mm4__chr4 8.03 Mbp_chr4_8025726 CTCTAGGACCCACAGAGGCTGCTCACAGGGGCTGCCTGGGGAGCGGGGTGAGCCTGCCCTCGGCTCTGGCCACTTCCCTC
TCAGGGACTCACAGTTGGGAC
mm3__chr11_KI270721v1_random 53.14 Kbp_chr11_KI270721v1_random_53139 TTTTCTAACTGGGGTGGCCCCGCCCAGAATTCCCGCCCCTGCCCTGCCGGCCAATCAGAGCAGGGCCCTCCCGAGGGCCC
CCGCAGGGCCCACCTCCGCCCTGGACAGTTCCAGCACACGTCTCTCTCACCCAGCAC
mm3__chr11 2.00 Mbp_chr11_1997957 TTTTCTAACTGGGGTGGCCCCGCCCAGAATTCCCGCCCCTGCCCTGCCGGCCAATCAGAGCAGGGCCCTCCCGAGGGCCC
CCGCAGGGCCCACCTCCGCCCTGGACAGTTCCAGCACACGTCTCTCTCACCCAGCAC
mm3__chr16 27.81 Mbp_chr16_27811500 CCCTCCTCATCCCGGAACACCACCTCCCTGGCCACATCCAGCACCTCTAGGTCAACAGAGGCCTGTCCTATTGCATAAAG
GGCTTCCAGCATCCTC
mm3__chr13 45.83 Mbp_chr13_45832051 ATGAGTGCTAGGGAGAGCCAGCCGGAAGTGCCTAGGGAGGAGGGAAGAGAGGTGTGGGGTGC
mm4__chr17 46.02 Mbp_chr17_46021003 CCACCAGGCCACTCAAGTAGCAGCTGAGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGT
TCTGAACAGATGGGCACCC
mm4__chr9 136.84 Mbp_chr9_136843432 ACCTTCTGAGCGAAATGGGGAGGGCCCTCTCCTGCAAAGCAGTGGGGCTTCCCCCACCCTCCGCCCTGCCCACCCCCAGC
GGGCAGCTTCCAGGCCTGGTGCGCAGCTCAGAGGAG
mm4__chr1 247.11 Mbp_chr1_247111744 CTTGGAGCTCGTAGGCACCGCGCGCGGTGCGGGGAGCGGCCGGGGCGGAGGGACCCAGCGAGCCCGGGGAGGGCCGGGGT
CCTGCAGGCGGAGGCGCCGGGGAGGGTGTGGGGTCAG
mm4__chr17 36.71 Mbp_chr17_36706306 CCTACGTGTGTGCTCCAAGTCTTGAGAGAATAAAGGCTACTTCTTAGATCAGACTGGGACCTCTCTGTTCCTGGCCTCTT
GCCCCCGCCCTGCCATCTGCCGGCCCCAAGAGGGCAGCACAGGTGGGTCTTTTCT
mm4__chr9 134.97 Mbp_chr9_134967700 TTTAGAGGGAGTGCGTCTGCGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTGTCAGCTCA
GAGCAGGAAACG
mm4__chr16 87.69 Mbp_chr16_87687531 AAGGCAGGACCATGAAGGAGCCTGGCCATGCGGTGTCCAGTGCCCAGGCCACTTCCGGTGCCATCCGCCCCCGCCCCAGA
GGCAGGAGGACTGAGCGTCGGAGGCTGCAGGACGCAGGTGGCTTGAAGGCACAGGAAGA
mm4__chr3 98.02 Mbp_chr3_98024575 GCCTATTGTGCTTCCCTTGGTCATAACCACATCATGAGAAGAGTATCCCCCGCCCTGAACACTTGCGGTATTCGTTATTC
ATCAGAAGGCTGTATCTGTTATACTGCCAGAGGATCTTTCTTCTCACCC
mm4__chr8 23.53 Mbp_chr8_23528951 GAAGTGAAGTTTTGCGCCCCTCCCCCTCCCTGCCCACCTCCTGCAGCCTCCTGCGCCCCGCCGAGCTGGCGGATGGAGCT
GCGCAGCGGGAGCGTGGGCAGCCAGGCGGTGGCGCGGAGGATGGATGGGGACAGC
mm4__chr17 82.78 Mbp_chr17_82784644 TTGCCTTGGGTCTCACTGAGGAAACATGAAGAAGGGGTAGATGGCAGTGGCCAGGGTGGGGGTCCTGGGAATTCTGGTAG
CTTCACCTTAATGTTTGTGATGCTGGGTGTACAGACAGCCTCCCACATGACCAAGGAC
mm4__chr19 49.58 Mbp_chr19_49580588 GGTCGCTTCTTCAACTGTGCCCCGACACCGGAAAGGAGCACTATTTTCCCCCTCCGCTCCGCCCACTTCCGCCCAGCAGA
AGGCACCGGAAGTAGAGCTGCCCCTAGTTGC
mm4__chr18 36.53 Mbp_chr18_36532337 AAACTCCAGGACCCATACGCTCCTCTTCCCTGGCCACTTCCCTCAAGTCTCACCCTCCCTGGAAGAGAGAAGGAAGAAAA
GGGACATCCATAGTGGCTTCTGTGGCACC
mm4__chr11 62.04 Mbp_chr11_62042410 GTAAAGGCAGGTGGGGTGAGTTAGAGTGGTTGCAGTAATAGTGGCACACACGCTGTGCCACGGGGAGTGAGCTGGAGGTG
CCCAGGGCTGGGGCCGCCCTATAATGCCAGCTGGA
mm4__chr4 24.65 Mbp_chr4_24650161 TCTTTTGGTGGCTCAGTGGGTCACAGCAAAGCCTGGATGTGGCCAGGGTGGTGGGTGCATTTTGTCTGTGGTAGGAAAAA
GTGGAATACATACCAGCAGACTTAGCAATTGTGTGCCCCTCAGGAG
mm4__chr3 197.03 Mbp_chr3_197027939 CACCTCCATGCCACCGAGCACTGCGGGCAGGGGACCGTGAGGCCCGGCTCCCCCGCCCTGCCCAGCCCCGGCCCACCATG
GAGGGTGGCTCCAGCAGTTCTGTGTGAGCCCAT
mm4__chr14 93.71 Mbp_chr14_93706542 TATGATGGGTTTGGGCCAGGACGACTCTAGAAAACACGGTGCGATCCCCCGCCCTGGGCACTGCCAGAGTAGAGAGTTTG
CCTTCTTCAGGCCAGACAACCCTTGAGCCAAGCCTA
mm4__chr4 146.19 Mbp_chr4_146192870 ACAGGACAGTGGAGAGAGCCCTCTCTGATACCTGTTAGTCCCTCCTTTAAAAGATGCGGGAGGTGGCCAGGGAGTGGGGA
TTTCAGAATGTGGTGGCAGAAATCAGTTTGGCATTTATTGGATGACAGCTGTAGGCAGG
mm4__chr13 113.08 Mbp_chr13_113080729 CACCTGCTGTGTGTGAGGGCGTGGGCAGGGGGCAGCTCCGGCGATGCCAGACCCCCGCCCTGGCCACGAGGGGCCACACA
GATACCCACCACGAGGA
mm3__chr12 109.83 Mbp_chr12_109828321 TCCTGTGCGCCTGGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGAAACCAATGCTCAGG
mm4__chr18 37.34 Mbp_chr18_37336985 GCTGCGTCCCACTTCATTTCTTTCCATTTCATTGTCCCTCCTGGGCCCTGGCCGCCTCCGGCTGTGCTCCACTCACCATG
CCTGGCGCTCACAGGCCTGGTGGTCTGCCTGTCGACCCTTCCATGCTAC
mm4__chr17 81.47 Mbp_chr17_81468256 GACTAGGGGTCCAGGTGGGGCTGAGGGCCGGAAACGGCCAGAGGGGAGGGGGCCTGGCTGAAGGGGGTGCCACCAGTGAG
CCCAGGAGGCAGGTGACCCGGGCTTAGCGAGTGGGGTTGACGGCAGGCTTAGGT
mm4__chr14 55.13 Mbp_chr14_55125735 ATGTGGGTTTGGTGATGGCTAGTGCTGAAGACAGGCCTGGGTTAGGCTTGAAGGACAAACAAGGAAGGTTAGGCTGAGGT
GGCCAGGGCTGAGGTTGGGCTGGAGAAGGGGTTTTGCACC
mm4__chr14 100.50 Mbp_chr14_100503682 CCCCGGCTTCACTTACACATCACAACCTTGTCCCCAGCCCTGGCCACCTCAGCCCCCGTCCCCGAAGACTGCTGGATTCC
CGTTG
mm4__chr12 27.78 Mbp_chr12_27779984 GATCCAGCCGGCAGGTCAGGCCGGCGGCTGGGGTCCCGCGGGCGGAAGTGAGAAGGGCGGCGGGGGAGGAGGCTGCTGGC
TGGGAGCTGGAATTC
mm4__chr3 50.37 Mbp_chr3_50371931 GACAACTCAAGCCAGTGGGAGTAGGGCACATCCCCCACCCCCGCCCTGGCCACTGGAGGTGGCTCTGATCCCTTTATCCC
CTTTGCTGGACTAGGCTGGAC
mm4__chr1 21.26 Mbp_chr1_21260925 CTTACAGCCAACTCCTGGGGCCGTTTTCCACCGCCCTTGCCAACTCCAGCAAGCAGCATCTCCCTCCTGCAGTGGCTTCT
CAGACCTT
mm4__chr1 109.79 Mbp_chr1_109792708 CCAAGGGAGTCAGCATGGAGTGAGGGGTGCATGGGGCAGTGGCGGGAGCTGGCCTGGGCGGGGGCCTGGGGAATTCCAGC
CCAGGAGAGCCAACTGGAAGCACATGTGTTTAGATGGGCACGGAACCC
mm4__chr6 43.48 Mbp_chr6_43477555 GCCTCAGATGACGCGACACGCCAAGTGCGTGGGGATTCCCGCGGCCCTGGCCACTACCCGGCCTGCCCCGCGGCAAGATG
GCGGCCTGAACGCAGCTGGCAGCGGCGGAAAGTTTGTTGTGGG
mm4__chr11 8.39 Mbp_chr11_8386542 CCTCTCTTCTCCTGGGACCAAGTGGCAGGCAGTGGCCTGGGAGGTGGGCAGCCAGGGGGAGGTGGCACCATCCCCCGCTG
CTGGCTTAGGCCCAGAGCAGACGTCTCCCGTCCTGCACCACAGAATCCGCGGAGCCTATG
mm4__chr10 60.29 Mbp_chr10_60287344 AGCTTTCCCTACAGCTGAAAATATGGTCACTTTCTTTTTCCTCATTCATCCTTCTCCCTGGCCAGTTCAGGCCCTTACTG
TATAATGTCCAGATCATTTAAAAAATCTCCAGGAGGCTGTAGTTGGCAC
mm4__chr22 45.65 Mbp_chr22_45648576 GTTCCAACTGCACTCCGAGATCCCGCTCCCTGACCACTTCCGACGCCGCCAGGCCTCTCACGCCTCCGTCCCACCACACC
CCCTGACCTCGTCCTGATCTCAGCT
mm3__chr2 37.67 Mbp_chr2_37671345 TAGATGGGAAAGCGATGCCCTGGGTATCCCGACTCCCTGCTGGTTTCCCAGCCTAACACCAGCGCACGCCGGAAGCGGCC
ACGGCCGCGGGCGGGACAGACTCACAGACAG
mm4__chr1 8.22 Mbp_chr1_8217376 GTCAGCCAGGCCAGCTTGTGGGGGAGCGGCCGCCCGCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNGGCTTCTCTCCCCGCAGC
mm3__chr1 159.98 Mbp_chr1_159983899 GGGAAAGAGACCCACGTCACTCCCAGCAGAGTCCTGCATGGCAGCAAGGGGCCAGGGCGGAAGAAGAACAAAAGACACCT
GGAGAAGAAAGGCAGTGCCTTTCCCTGCAGCAGCCAGGGCTGCAGATATGAT
mm4__chr10 92.57 Mbp_chr10_92574162 GGTAGCTGCTCCGGGTTTTGCCTGGAGCCGGATGGGGCCAGGACGCAGGCGAGGACGGTGAAGACCGCGGACAGCCGAGG
CCTGAGCAGGCGGACTGGAGGGACAGGGGTTT
mm4__chr19 55.45 Mbp_chr19_55454561 TGGCCAGTGGAGTCTGAGAAACTGGAGGAGGGGCACCCGGGCCGGAAGGGGCAGAGGCGGAGGAGATGGAGAGTTGGAGA
TGGGTGTGGACGGGTCAAGGGGCTTCTATC
mm4__chr9 133.38 Mbp_chr9_133376657 GTCGCAACCCTGGAGCTACGGCCGGCGGTTCCGACCGAGGGCGGCGAGGGGCCCGCGCCCTGGCCAGTGTCGGCCTGCAG
CTCCTAGGTTGAACCCG
mm4__chr14 24.13 Mbp_chr14_24131388 CATACTACCACACACCCGAGGCCAGGACCCTGCTGACGTGGCAGACCTCCACCCTGGCCCCTTACTGCCCCCCGCCCCTC
TCCCCCACCTGCCAGCTGCCAACAGGGCTCTGCCTTGTGTCTGCCACACCTGTCACTG
mm4__chr17 6.56 Mbp_chr17_6556123 GACATCCCCTTCGGTGGCGAAGCCCGGGTCTGCCCGGGGCCTCCGCGGGGTGCAGAGGGCTCCCAACGGCGGGACTGGCC
CGGGGCGCCGCAGACCTGGCCCTTTCCGGCGGGGCCGTGGAGAAGGGGACGCGGTGTC
mm4__chr5 135.54 Mbp_chr5_135536013 TAAGTACCCGGCGCAACAATGGGCGCCCCCCTCCCTTGCCACCTCCGCCCCCGCGGCAGCCCGGGTGAATGGAGCGAGGC
GGCAGGTCATCCCCGTGCAGCGCCCGGGTATTTGCATAATTTATGCTCGCGGGAGGC
mm4__chr16 1.51 Mbp_chr16_1508090 CCCTCCTTTGGTCCTGATCCTAGACCCTGTTGGGGTCCCTCTGTGACCCCGCCCACCCGCCTCCCTGGGCCCCTCCGGCC
GGTGCAGTCCTGGCAAC
mm4__chr14 90.74 Mbp_chr14_90743862 TGAGAATCTGAAACAGGCATGTTGCCTCCACCTCCCTGGCCCCATCCAGCCTCCAGCCTCCAACCTCCTCTGTCTGAGGC
TGCGAGCTTAGAACCCCGAGCTC
mm4__chr1 46.41 Mbp_chr1_46410959 CGAGTCTGGGTCTGGGTAGTTTCTGACAGGAAAGGACTTGAGGGAAGTAGCCTCTGAGGCTGGAAGTGGCCCAGGCAGGG
GGGCAACCTTTGTGGCCTTCAG
mm4__chr9 129.48 Mbp_chr9_129483250 CCGGATCTGAGCTTCGTCATTCACGCCCCTCCTCCCTGGCGACTTTGGGCCTGGTGACAGTCCCCCGACCCTCGCAGAGC
CAGGGGCACAGTAGGCGCCTTAGCCACTGGCAGAATTTCACCG
mm4__chr17 43.99 Mbp_chr17_43994858 GGATCCGAGACAGAGCCCTGCCTGGGCCCAACGAACGGGAGGGCGCTGGCTGGGGCCGGGGGTGGCCAGGGCCCCGGGGG
AAAGGCGGTAAGCATGAGCA
mm4__chr6 41.68 Mbp_chr6_41679791 AAAGGCCAATACCTGCACCACCCTCAGCCCTGACCAGGTCCGGCCTGTCTTCTTTCATGCCTAACAAGCCCCTAGCTCCT
TGGCCACTGCATGGGTGAGAGGAGAGGGA
mm4__chr17 77.96 Mbp_chr17_77959424 GCCCTTTGTTCGGCCGAGCCGAGCCGGGGCGGGCGCTGGCCCCTAGCGACCCGCGTGCGGCGCCAGGCCCCGGGCCGGCC
GGGTTTGAGGGGAGCGGCGGGAAGCGGCCGGGGCCGGGGCGCGTGTGTGCTAGTGTGAGA
mm4__chr11 1.37 Mbp_chr11_1370765 ATACAGGGCAATGGAGCAGGCTCAAGGACGGCCGTGGCCAGGGCTGGGGATTGTTCTGCTCCGAGTCCCATC
mm4__chr19 15.12 Mbp_chr19_15117052 ATCTCCAGTTCAGGCCTCCTCGCTTCTGTCCTGGCCATTTTGAGCAGCCCCCCTGGCCTCCACCCAGCCCCCTTCCGGCC
TGCCGTCCATGGCTGCTGAGTGGACCTTCCCAACACAGATGCAACGGTG
mm4__chr4 965.54 Kbp_chr4_965537 ATGTGACTCACGGACACCACGGTGGCTGCAAAGGCAGGCTGTGGTCAGGGCGGTGGGAGGCGAAGGACACACAGCACTGG
GGCCAGGGACAGCCCCTCCGCCTGTCCAGGGGTGACATCTCACCCCAAAGGCAG
mm3__chr20 34.87 Mbp_chr20_34872847 CTGGCTGGCCTCGTTCTCCGCCGCCATCCTCGCCCGCGCCCCCCAGCAGCGCAGCGCCTGCCGGAAGTGGCTGCGGCGGG
GGAGTGGGAGGGCGGAGGGCGGGGCCTGATCTTCGCGACAGCC
mm4__chr3 24.83 Mbp_chr3_24830505 CTAAGGATGCTCGGGTGCTGGACCGCCCGCTGTGCCTCCACACCCCTCCGCCCCGCCCCCTTCCAGCTCTCAGCGCGGGG
TGAGTTTCCCTGACACTCCCCGCCGGAGGCAAGCAGGCAGAGGCGAAGTGATGGTCG
mm4__chr16 67.55 Mbp_chr16_67546051 GAGGATGAGGACGTGCAGACTCGAGTGGCTGGCTGCCTGGCCCTAGGCTGCATCAAGGTGACCCCTGCCAACCCTCCCAC
CCCTCTGTCCGCTTCCGGCACCCCCACCCCTGAAACCCTCCCATCTGCTCC
mm4__chr1 3.07 Mbp_chr1_3074112 GGGAAGACGGTGCAGAGAAACGGAGGCAGAGGGTGGGGGTCTGGCTTTCCAGGGTAGACCCGCCTGCCCAGTCTGATCCG
CAGCCCCGGCCCCTGCCGGCAACCTCGGGAGACGGGCCGGGCTCCCTAGTGCACTTG
mm4__chr19 2.43 Mbp_chr19_2426785 CCGAGATCCAAGCTGCACTGGCTGGCAGGGGGCAGGGCGGGGGGTGGCGAGGACACAGTCCCGTGTGTCCCAGCACCGGT
GCCACCCTCCTAAGCCCCGGGCAGGCAGTACGTACATGCGGACCC
mm4__chr7 10.38 Mbp_chr7_10379294 GTCCAAGAAGTGATCATTGCCCAGAAGAAAGAAACTGTCTACGAAGCATTTCTTTCCCCAGCCATGGTCACTTCTGGCAG
TGCTGCTATATCACTTCTATTCATCTTTCCCCCAAAAAACCCCACATGCATTCCTGT
mm4__chr7 76.26 Mbp_chr7_76260918 AGTCCGAATGCAGGTCAGTGGGGACAGAGCTGGCTGGGGCCAGGGCGGGGGATGTCTGTGGGTGGGAGAGATTCCAAGGC
mm4__chr20 31.50 Mbp_chr20_31496954 CCCGGAGAAACTGTCTGCAATGTCCTGCCGGGAGAGGCCAGGGGGAGGGAACTGCTGGGACCGCAGTGGGTGAAGGAGCC
GGAGGGGCAGATCCGGGAGGATGAGGATCTGGCATCTGTCCAGG
mm4__chr19 954.37 Kbp_chr19_954365 CCCAGCCTTGGTAATTAGGGGTCCACACCTTCCATCCCCCACAGCAGGAAGTGGACAGAGGGGAGGGGACCCCCTCAGGG
AAAGACCCCCAGGCCTGCCCTGTGGCCCCGCATTTTCTTGGCATC
mm4__chr19 44.99 Mbp_chr19_44985971 TACATGTGGGGTGGGAGGATCCTGTCGGTGGCCGGAAGGGTCCTGGGTGGCGGAATCTCTAAGCCTAGGGAGGAGAGCC
mm4__chr1 16.09 Mbp_chr1_16089338 GAGCCACTAGTCAGGACTGCATCAGGAAGCAAAGGGGAGCTGTTGAAGGATTCAGCACTGGGGCAGGAGGTGATCAGATG
AGAGTGCAGGAAGGGGCCAGGCTGGAGGCTAGAGGGAGGTGGGTAGAGTCCAGGT
mm4__chr14 105.50 Mbp_chr14_105498879 TGCTACGTCGACTACAGGGACAGTGTCGGCAGGAACTGGCCAGGCTGGTGGGAGCCCGCCCTGGTCTCATCTGGATC
mm4__chrY 14.83 Mbp_chrY_14830269 GAAAACCCTGGTGGCTCTCTTTACTGACCATCAGTGGGTGGCCCCCGCCGTGGCCACCGCCGACCTGCACGCGCAGTACG
GCTCCCCCACCTACTTCTATGCCTTCTATCATCACTGCCAAAGCGAAATGAAGCC
mm4__chrX 5.90 Mbp_chrX_5903303 GGCTTCATTTCGCTTTGGCAGTGATGATAGAAGGCATAGAAGTAGGTGGGGGAGCCGTACTGCGCGTGCAGGTCGGCGGT
GGCCACGGCGGGGGCCACCCACTGGTGGTCAGTAAAGAGAGCCACCAGGGTTTTC
mm4__chr3 194.88 Mbp_chr3_194877809 TAGTCCGGCTTCTACCTGCTCCTTCAGCTGCATCTCTTGATTCTGCGGACCCCTCCCCACCTCTTAACCCCAGGCATCCC
CCTCTGCCCTGGCCACATCAAGCTACTTGCCAAGCCTCTTTCCC
mm4__chr1 159.84 Mbp_chr1_159840642 TGAGAGAGGCAGATGGTGGAAGGACTGACCACAGGCCTGGCCACTCCCAGCAGAAGGACAAGAAGTGCAGGGGAA
mm3__chrUn_KI270744v1 75.57 Kbp_chrUn_KI270744v1_75573 GAGCTGCCCTGCAAAGAGTATGATGTGGCCCGCAACACGGGCACCTACATGTCCTCCGGCCTGGCCACCGCCGGCTGGAG
GTGTGGTTCCAGAACTGCTATGCTCGCTACCACCAGG
mm4__chr3 43.11 Mbp_chr3_43105612 GAGGGCTCGAACTGGCAAGCCCCAGGGCGTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNTGAGGGTCCACCCCCGCCCCGCCCACCTCCCGCGCCTCCCTCAGCTCGCTTCAC
mm3__chr2 241.95 Mbp_chr2_241953839 CAAAGGCCTGGAGCAGATGTACCCAGGCCCCAGGGAGGAGCCGGAGGTGCCCAGGCTGTGGGGAGGAGCCGGAGGTGCCC
AGGCCGGGGGGAGGAGCCAGATGTGCCCAGGCCGGGGCTGTCCTGAGGTCTCCGCT
mm4__chr8 143.29 Mbp_chr8_143289557 CTGGCCCTGCGGTCTGAGCCAGCGGGGAGAGGCCCGGGCGGAGGAGGCTGCGGACTCCTAGAGGTTCAGTGCCGGAAGTT
mm4__chr2 70.79 Mbp_chr2_70790685 CTGAGGCCGGGCTTTCACCTGCGCACCCGGGGCCGCACGAGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAC
CTGCAACCATTCCCCGGCTGCGTATTCCAATCCCGGCCTCC
mm4__chr14_GL000194v1_random 58.71 Kbp_chr14_GL000194v1_random_58710 AGCAGTTCTGGAACCACACCTCCAGCCAGCGGTGGCCAGGCCGGAGGACGTGTAGGCGCCCGTGTTTCTGGCCACGTCAT
ACTCTTTGCAGGGCAGCTC
mm4__chr7 157.43 Mbp_chr7_157428888 CCTCTGGAGGGACCTGGATTGCCGGGGCCTGAAGTGGCCACAGCTGGGGCGGGGGGCCAGTTTCCCTGGGTGCTCTCTGC
ACCTGGGAGGGACATCCCAGGACATCCCCCAGTCTCACTGGGAAATCTGCCCAC
mm4__chr10 116.77 Mbp_chr10_116774827 CAGTCCCATGCCTGCCCACGTGGCCCTGGCCTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNCGGCTCCTGCACCAGCCCCTCGTGTTGGCTATA
mm4__chr8 141.93 Mbp_chr8_141925408 TGTGTCTGGATCCTTCCCCTTCTGATGCTTCTGTGGCCCCGCCTGGCTCCCTCTCACCTGTGCCCTCAGACAGAGCCAGC
ACCGCCCCCTCCATGGCTACTTCCTGCCCTGTCCTCACCATGGCTG
mm4__chr22 49.93 Mbp_chr22_49934848 CTGCTCTGCACCAGTGAGGGGCCGGGAGTGGCCTGGCCTGAGGAGGCTGCAGGGCACCCACCTCTCCAGAGACCCCCTCC
ACCCACTCCCGGGGGATCCTTCTGGAGAGACCGTTCCCA
mm4__chr2 232.49 Mbp_chr2_232487664 AGAGGACGCAGACAAAGCCGGGAGGCTCCGCGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NAGGGACTCGGGCGCCACTTAC
mm4__chr1 3.42 Mbp_chr1_3416083 TCAGGATGAGAGCATGCCTGCCTCGCAGGGGCGGGAGAAGCCTGACCCCCACAGCCTGGGTGGGGCTGGAAGGGGCCAGA
GGGGAGGCCTTGGCCACTGTGGACGGACACGAGTTTCT
mm4__chr20 62.49 Mbp_chr20_62487805 GCCTTATCTGGCCGCCTAATCCCCTGGCCTTGGCTGGAGAACACCAGGGCTTTGCCCAGTCCGCCCTCCCTACCCCCTCC
CTGGCCCCTGCAGGCCGTTGGGGCTGGGGGTGGAGGGAGAGGGAGGTGGAGTGAA
mm4__chrY 11.16 Mbp_chrY_11158919 CCTGGTGGTAGCGAGCATAGCAGTTCTGGAACCACACCACCAGCTGGCGGTGGCCAGGCCGGAGGACGTGTAGGCGCCCG
TGTTGCGGACCACGTCATACTCTTTGCAGGGCAGCTC
mm4__chrUn_GL000218v1 43.91 Kbp_chrUn_GL000218v1_43905 CCTGGTGGTAGCGAGCATAGCAGTTCTGGAACCACACCTCCAGCTGGCGGTGGCCAGGCCGGAGGACGTGTAGACGCCCG
TGTTGCGGGCCATGTCATACTCTTTGCAGGGCAGCTC
mm4__chr21 9.07 Mbp_chr21_9073477 CCTGGTGGTAGCGAGCATAGCAGTTCTGGAACCACACCTCCAGCTGGCGGTGGCCAGGCCGGAGGACGTGTAGACGCCCG
TGTTGCGGGCCACATCATACTCTTTGCAGGGCAGCTC
mm4__chr2 94.87 Mbp_chr2_94871655 GAGCTGCCCTGCAAAGAGTACGACGTGGCCCGTAACACGGGCGCCTACACGTCCTCCGGCCTGGCCACCGCCAGCTTCCG
CACCTCCAAGTACCTGCTGGAGGAGTGGTTCCAGAACTGCTATGCTCGCTACCACCAGG
mm4__chr4 164.60 Mbp_chr4_164596440 CTCTGTGAGTTGGGAGTGGGGGTCTGCGCCCTCCTTAAGGGCATGATGTAGGGGAGGCTGGTAGTGGCCATGGCAGTGGT
TTGACCAGACCCAATAAAGGCGG
mm4__chr8 13.10 Mbp_chr8_13099785 TGGAGCTGGAAGAATTGCGTCTCTTCAGTTCCTTGGGGCTGTCGCTACTGTTTTCCCTCCTGAGGCTGATGTGGCCAGGG
CCGTGGAAGCTTCCCGTCCTCCAGTTCACAGAGCCGTT
mm4__chr9 128.39 Mbp_chr9_128393203 CTTTCCTGTGGCTGAGTCGCGGCCGGGGCGCCTGCGGTGGCGGTGGCGGCGGTGGCCCGGGCGGCGGGGAGTGCTGGAAT
GCGCC
mm4__chr6 148.78 Mbp_chr6_148778572 TTGTGAATGGAGCATGGCCTCCCTTTCAGCCCCTGACCCCTCCTCCCAGGCCACGTCAGGCAGGGCACTCTGGTGTGGGG
TGCAGTCCTGTGACAATCCCTGCATCAGTGTGGG
mm4__chr19 4.72 Mbp_chr19_4723760 CTCCATGCCCGGGACTGCGACCCCGGAAGTGGCGGGAGCGGGGGACGACAGCCGCGGCGGACACAGGGGACCCGCCGGCT
CAGGCACCTTTGACCCGGAAGTT
mm4__chr10 124.62 Mbp_chr10_124616660 GGAATGACCTCCTTCACCCCTGGCTCCTTCCGGCAGCCTGGCCACTTCCAGCATGTGGAGGGGGCCCGGGCCCTGGCAGA
GACGGTGTGGAAC
mm4__chr17 28.52 Mbp_chr17_28524633 GAGCAAGTCCAGGGCCACTGCCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGACAAGTATCCTGGCTT
TGGCTTTGAGG
mm4__chr19 45.39 Mbp_chr19_45386011 GGGTCGTTCATCTGAGTGCACCGGGGGAGGGGGAAGACTCAGTCCCGCGGCTGGCATCTGCGATGCCCCCGCCGTGCCCA
CCTCCCGCTCAGCAGCGCTCACCTCCTTCAC
mm4__chr16 73.06 Mbp_chr16_73057984 GGTGGGGCAAAGTTGGGGGCCGGGAGCGGCGGGGGCGGGGGCACTCGGCACCGCGGACCCCCCCATACTCCCCCCACCGC
CGGCCGCCGCCTTTGTCTCCGAGCTGGTACC
mm2__chr20 50.04 Mbp_chr20_50040347 GGGCCTGAGACTCCGTAAACAGCCCTGGCTCGTAAAGCTGATCAAAGCAGCAGGCCTGTCCCAGCCCTGGCCACTTCCTG
CAGGGCCCAGAGCCCTGGCCTGTGGGAGATCCTGGGAAGG
mm4__chr2 130.31 Mbp_chr2_130314343 GACTGCTGGTGATCTGCGAAGGCCTGGTGGCAGTGGCCATAGCAGTTCTAGAACCACTCGTCCAGCAGGTGGTTGGCTGA
GCCCGCAGTGGCCAGGCCTGAGGAGTGCAGTCACCTGTGTTGCAAGCCACGTAGTCCAT
mm4__chr12 131.73 Mbp_chr12_131730749 CCTCTGCAGGAACCCTAAGCTTACTCTGTTCAGGCCCCTGACTACCACCACCCTGGGCACTGACGGCACCCCACCCTATC
CTCCCAACTGCAAGGGCTCTAGTAGGGGGTGCCCTCTCCTCCCCTCAATA
mm4__chr21 45.43 Mbp_chr21_45432256 GAAGGTCAGAGCTGGGGCTGGGGCTGGGCTGGCGGAGGTGGCCTTGGCGGAGGCCACATTCCAGGGTCTCAGTGAGAGTC
TGTGGCAGG
mm4__chr1 156.12 Mbp_chr1_156115303 CTGAAAGCGCGGTGAGTTCGCCCAGGTGGCTGCGTGCCTGGCGGGGAGTGGAGAGGGCGGCGGGCCGGCGCCCCTGGCCG
GCCGCAGGAAGGGAGTGAGAGGGCCTGGAGGCCGATAACTTTGCC
mm4__chr16 33.71 Mbp_chr16_33706946 GTTCTCGCTACCCAGGGTTCAGTGGCCTGGGGGCTGACGGAGGGGGTCGCCTCTGCCAAGGCCCCTTCCGGCGCCTCCCT
GGCTCATCCAGCCCACCTCCCTCCCACGCTGGCTTACGCAAAGTGCTCTGGTCACCA
mm4__chrX 107.83 Mbp_chrX_107825920 GGTTTCTCCGGTCACTCCTGCCAGGGGAGAAGACATGTAAACGTGCCTCCAGAGGAAGGGCTGGGGGAGCTGGGAGTGGC
GGGGGCGGCGGAAGGTGAGCCTGGGAAGGTGGCCGAGCACCCCTTCTTCTTGGG
mm4__chr16 87.38 Mbp_chr16_87383317 CACCCAAAACACCACATCGCCGGGCCCCGCGCCCAACTGGTGGCCCCCGGCCGGGGCCACCGCCCCCGCCACTCCCAGCT
CCGAGGCCTCCACCTGGCAGGGAC
mm4__chr16 35.02 Mbp_chr16_35023241 AATAGCGCTCAGGACCCAGAGGCTGGACAGCCAGCCCGCCCGCCCGTCCCTGGCCACGAGGCGCCCCGGACCTGACCACC
GCCCCCGCCACCTCCGCCGGTGGGCAAGGAGGGTCCCCTTTCAGCCTGCAGTTCCT
mm4__chr16 34.39 Mbp_chr16_34388563 AGGAACTGCAGGCTGAAAGGGGACCCTCCTTGCCCACCGGCGGAGGTGGCGGGGGCGGTGGTCAGGTCCGGGGCGCCTCG
TGGCCAGGGACGGGCGGGCGGGCTGGCTGTCCAGCCTCTGGGTCCTGAGCGCTATTC
mm4__chr4 183.48 Mbp_chr4_183483481 CGAATCTTGGTGGCCGCTGCTGGAGCGCGGCCTGCGCCATGGCCACCTCCGCCGCCTCCTCCGAGCGTTTCAAGAAGCTG
CACGAAATCTTCCGCGGCCTCCACGAAGACCTACAAGGGGTGCC
mm4__chr19 7.83 Mbp_chr19_7832115 GTGTCCCTGTGTCTTTCCGTCTTTTGTGGCTCTTCCCATCCATCCCGCCGCCCTCCCCACTCCCAGCTCTGGCCTCAGGG
TCCAACCTGTAGGTTCCTCTGGGCTTCTGTG
mm3__chr3 13.93 Mbp_chr3_13933044 GGAGCCCTGAGTCCGGAGCGGAAGCGAAGCCGCGCGCTGCCCCGGCCGCCGCCCACGCGACTTCCGGCGGCGCCGAGGCA
CGCGCGCCATAAGTTGGAGCTCCCGGGGCGCCCGGGACGAGCTTCCCAGGATTC
mm4__chrX 9.50 Mbp_chrX_9495276 TTCTCTGTGATGGAGCGCAGCTATCCTACAAGTATGTAGCCCTGATTTAGTAGAGAAGAGGCAGGAATTGTCGAGGGCGG
AGGAGCAGGAGAAATGGTGCACAAGGGAGAGG
mm4__chr17 19.22 Mbp_chr17_19223313 GCCCAAAATAATCGAGTGTAAGGCAGGCCAGAAGTGGTCAGGGGGCAGGGACAGTCCCCAGCAGGGTGGCAAGTCTTTC
mm4__chr17 19.15 Mbp_chr17_19154917 GAAAGACTTGCCACCCTGCTGGGGACTGTCCCTGCCCCCTGACCACTTCTGGCCTGCCTTACACTCGATTATTTTGGGCA
AAAAGTGCAAATCAAGACTGAGATGTCA
mm4__chr17 19.10 Mbp_chr17_19097146 GCCCAAAATAATCGAGTGTAAGGCAGGCCAGAAGTGGTCAGGGGGCAGGGACAGTCCCCAGCAGGGTGGCAAGTCTTTC
mm4__chr8 142.40 Mbp_chr8_142403288 GACTACCCGCCCCAGAGGCCCCGGCGCGGAGCCGGAAGCGGCGCGGACGGCGGTCGGCTGGGCCAAGAGGCCTCTGCGGG
TGCGCGCGCGAGTCGGCTGTCCTCACTTGCCTGGTG
mm4__chr4 6.73 Mbp_chr4_6732843 GTCAGGGTGACTCAGCTGTTCTTGCAGCTGGGCGTGGGCTGATGGCTGGGAGCTCTCCAGGCAGCCCGCAGTGGCCAGGC
CGCAGGAAGGCAGGCGGGAGCAGCCCGGGAGGACTCACCAAGTTC
mm4__chr9 133.43 Mbp_chr9_133428578 GAAAACTCGCTGGCGCTGCGGCACTAGGGCGCCGGGCCGCTGACTCGCCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGACCAGCTTCGGCCTGGAG
mm4__chr8 22.06 Mbp_chr8_22056747 CACATCCACACACACACCCTTGCCAGCCACACGCTATCAGCTGGAAGTGGGGAGGGCGCGGGGAGGGCGTGGGGGAGCCA
GCAGCCCGCCCGCCCTCCCGCCCCCAGACGAGGACTCCCCA
mm4__chr9 99.82 Mbp_chr9_99822140 GCTCACTTTGCAACGCTGACGGTGCCGGCAGTGGCCGTGGAGGTGGGAACAGCGGCGGCATCCTCCCCCCTGGTCACAGC
CCAAGCCAGGACGCCCGCGGAACCTCTCGGCTGTGCTCTCCCATGAGTCG
mm4__chrX 49.22 Mbp_chrX_49219604 AGTTCTGGCCCTGACACAACACCCATGCCCCCACTCCCACCTTGGCTGCTCCTCCATGCTCCTCCACCTGCCCTAGCCCC
TCCTGCCTCACCCCCTGCCACTTCCGGCACTCACGGGTTGGTTTGGCTGAGGCAGAA
mm4__chr19 7.47 Mbp_chr19_7470220 TCCGAGGGCTTCTCTCTCAAGGCCGGGGGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCGCCACTGCCGGCCACACC
ACTCAGCGCCAAGGAGGACGCCAGCAAAGAAGAC
mm4__chr18 7.57 Mbp_chr18_7567446 GGGCTGCCTGTGGAGAGGCGGCGGGCGGAACGCGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGCTCG
GGTCCCGGGTCCCGGGCAGGGGAAGGGGAGAGGCGGCGAGCTCAGCAACCGGAACCGAG
mm4__chr8 64.58 Mbp_chr8_64581051 TGCTTCGAGCCGGCTGACCCCGAGGGGGCAGGGCTGCTGTTGCCGCCGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNGGTGGGTGTCCCCGGGCTGCTAGTAGGTTCAG
mm4__chr1 20.66 Mbp_chr1_20661515 GGAAGTGATCGGGATACGCCGCGGCTGGGCGTCCAGAGCCGCAGCTTTGGCGATAAGGCGCGGAGAGCGCCGCCGGAAGT
GCCCTGGTGGGAGGTGGATTCCTTGAACCCTATTGGTGGGAGTGAATCAAGGC
mm4__chr9 93.22 Mbp_chr9_93218344 CTGTGTCCCTGGTGCTATCCAAATCTATCTTTAGGTGTCCCTGCGGGAAGTGGCCATGGGGTGGGGCTGGCCCTTTCTAG
AAACCAGATACATGATGTGGTCAGATGCAGGG
mm4__chr16 79.56 Mbp_chr16_79564090 GAGACCTTTCAGGCAGGTGGGATGCATTTCAAGTGGAGCAGACCCTGCTTTCAACAGATTCCCTCCCCCATGGCCACTGC
CGCCCCTTCAGGCCTCCTGGGATTTCTGCTA
mm4__chr6 165.86 Mbp_chr6_165855293 AATGAACAGAGGCGGATGGGTGGAGCAGGTGCTGGTGAGGGCACCAGCCTTCGAGGGGACTCATAGGCGGCGCGGGAAGT
GGCGGGGGGGGGGGGGGACTCAGGGGCGCTGCCTCCTGTGGATCACCTGGTAGC
mm4__chr8 143.29 Mbp_chr8_143291347 GGCAACCGATGAGCTGAAGGCGCCGCGGCTGGGCCGTCCCGCTCTCCCGCCGCCGAGCGCGCTGTCCTCCCGCTGCCCAG
AGCGTCGCCGGAAGTGCCGAGGGGGCGGGCCAGGGGTGGAGACTGGCGTTTC
mm4__chrX 140.09 Mbp_chrX_140091409 GTAGGAGCGGACCGTGGGGCCGGAAGGGGGCATCGCGGCGGCGCCGGCGGCTGCGGGGTTATCTCC
mm4__chr7 44.49 Mbp_chr7_44490648 GTACGCTTCACACACACAGCGCCGCCTCAGACCTGCCGACTGGCCACTTCCGGCGTCCGCAGCCAACGGCTCCGCCGGAC
GGCGCGGCTGCGGAACTTCCGGTCCGTGCTC
mm4__chr3 127.28 Mbp_chr3_127284474 GACTAGGTACATGCCTGGCCCCACTCTCTCGCCCAGGGCCCTGAGAAGCCCAGCCCCACTGCCGCGGCCTCGACCTGCCC
GCCAGCTTCCTCCCCCACGCTGGCCTCTTCCTGCAGGAGGAGCTGGCTGTGC
mm4__chr19 3.97 Mbp_chr19_3971050 CGCAGCCCGGAGAATACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTGCCCACCCCCGGGAAACGAACCCC
mm4__chr18 23.45 Mbp_chr18_23453175 GCTGATGCTTGCTTCTCCGAGCCCGGCGGTCAGCAGCCGGAAGTAGCCCGCCCGGGGGCGGGGAAAGGTGCCTTAAGGAG
GAGCTAGGGGCGTGCGGCCAATTGCAAGCGACTGTCCTAGCGAA
mm4__chr16 23.54 Mbp_chr16_23535202 CAGGTGGTATGTGGGGAAGCCGTCGCTCTTCATGATGACTGGGTCTCCCTCCACGCTGGCCACTTCATGCCTATTCCAGC
CATAGACCAGGTCCTG
mm4__chr12 54.47 Mbp_chr12_54473592 CACAGTCACCTTCCTCCCACGCAAGCCCCAGCAACGGCCACTTCCGGCGCCTAACGTTGGGAGGTGGGGCGAGGGGCCGG
CACTTCCTGTGACTGGGAGTCTCGGTTCTGGCTGAG
mm4__chr10 31.32 Mbp_chr10_31318844 AATCAGCCAGATCCCTCCCTGCCCCGGGCAGCCGCGGCGGGTGTGGCCAGCGCGGAGGCAGGACGCCGCCGAGCCTCCAA
CTTTACCTTTCCAACTCCGACAGC
mm4__chr10 122.05 Mbp_chr10_122051027 GAGGCTGGTGGATGAATCCCAGAAACCAACTGTGGCAGTGGGATGTGGCCAGTGCGGGGGTGGCCCATCAGTCACTTCC
mm3__chr7 27.10 Mbp_chr7_27097013 CTACTCGACCGCCGGATTTTTAGGGGTAGGAGGCGGGGGAGAGAGATGACGCTGGCGGACGTGGCCAGCGCGGGGGCCGG
GCGGTGCGCTGCAGGCCATCTGCCGGCGCCCTGAGACCCAGGAGCCTCC
mm3__chr20 37.41 Mbp_chr20_37406611 GTGGATGTGGGTGGGAACAGCATGTGTGCCCGTGGACCCGTGTGAGCTTGGGCTCGCACCCCCAGCGCTGGCCACTCCCG
GCGGGACCCACGTGTGCCCGGGGGCGCAGGGCCACGTGCGAGCGCAACCGGGACAAAG
mm3__chr20 32.99 Mbp_chr20_32985581 ATCCCCTACCCTTGAAGGCTGAAATTGGACCCCAGCGCTGGCCACTTCCAGCCTTAACCAAGCTGCATTCAGCCCCTCCA
GCCTGCAAGTGTTGTGCAGAC
mm3__chr11 809.53 Kbp_chr11_809531 AACCACCAAGGAGTCAAGGCGAGCCTACCGCCAGCAGCTTCTGCCCGGGCCCACGCGCCGTTCCGGGTAGGCCCGCGGCG
CAGGCCACTTCCGGCAGCGGTGCACCATGGGACTTGCAGTTCGCTGGCAAAGGTTGAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.