← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529657_F | TCGTCGGCAGCGTCAGCGCGGCGATATCATCATC | 60.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529657_R | GTCTCGTGGGCTCGGCTGGCGGCCTAAGGACTC | 59.4 | 1.00 |
| mm3__chr1 107.27 Mbp_chr1_107273211_F | TCGTCGGCAGCGTCATCAGGCCTTGCTAGATGGC | 59.8 | 0.52 |
| mm3__chr1 107.27 Mbp_chr1_107273211_R | GTCTCGTGGGCTCGGTCTCCACTGCATCACTCAGC | 59.7 | 0.52 |
| mm2__chr1 981.62 Kbp_chr1_981622_F | TCGTCGGCAGCGTCGATGACCTTCCGATCGCCTT | 59.8 | 0.46 |
| mm2__chr1 981.62 Kbp_chr1_981622_R | GTCTCGTGGGCTCGGCTCTCCGTGCTGACTTGGG | 60.0 | 0.46 |
| mm4__chr19 36.42 Mbp_chr19_36417593_F | TCGTCGGCAGCGTCCGCTCGGACCCCTTTTCTTA | 59.7 | 0.45 |
| mm4__chr19 36.42 Mbp_chr19_36417593_R | GTCTCGTGGGCTCGGTCTGGTTATGTGTGGGTGGG | 59.3 | 0.45 |
| mm4__chr12 22.85 Mbp_chr12_22850013_F | TCGTCGGCAGCGTCCTGCTGACGGTGGGTACATT | 60.0 | 0.32 |
| mm4__chr12 22.85 Mbp_chr12_22850013_R | GTCTCGTGGGCTCGGTCCTCAGACTCTTGGGTTGC | 59.3 | 0.32 |
| mm4__chr10 132.27 Mbp_chr10_132271362_F | TCGTCGGCAGCGTCCCCCATCTGTAAAGCAGGCA | 60.0 | 0.32 |
| mm4__chr10 132.27 Mbp_chr10_132271362_R | GTCTCGTGGGCTCGGGAACGTGGCCTCTGTTCTGA | 59.9 | 0.32 |
| mm4__chr6 51.62 Mbp_chr6_51620531_F | TCGTCGGCAGCGTCACTTGTCCTAAACCCTGGGG | 58.9 | 0.32 |
| mm4__chr6 51.62 Mbp_chr6_51620531_R | GTCTCGTGGGCTCGGTCTTCAATACATACAAATGCGTGGT | 59.3 | 0.32 |
| mm4__chr19 9.27 Mbp_chr19_9268051_F | TCGTCGGCAGCGTCAGAAATGGAGCCAAGACCCTG | 59.9 | 0.30 |
| mm4__chr19 9.27 Mbp_chr19_9268051_R | GTCTCGTGGGCTCGGGTGCATCTCTCCCTTTCCCC | 60.1 | 0.30 |
| mm4__chr19 9.20 Mbp_chr19_9199291_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4__chr19 9.20 Mbp_chr19_9199291_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4__chr6 11.11 Mbp_chr6_11111516_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4__chr6 11.11 Mbp_chr6_11111516_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4__chr7 152.13 Mbp_chr7_152134117_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4__chr7 152.13 Mbp_chr7_152134117_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3__chr6 45.21 Mbp_chr6_45207805_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3__chr6 45.21 Mbp_chr6_45207805_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4__chr17 33.31 Mbp_chr17_33310535_F | TCGTCGGCAGCGTCTGCATTGACGAGGACAAAGGT | 60.2 | 0.28 |
| mm4__chr17 33.31 Mbp_chr17_33310535_R | GTCTCGTGGGCTCGGAGGATCAAACCCTTGCACAGT | 59.8 | 0.28 |
| mm4__chr14 105.25 Mbp_chr14_105253314_F | TCGTCGGCAGCGTCGGGAGGACACTTCAGAGCAA | 59.3 | 0.28 |
| mm4__chr14 105.25 Mbp_chr14_105253314_R | GTCTCGTGGGCTCGGAAGCCTGGGATGAAGACAGC | 60.0 | 0.28 |
| mm4__chr2 146.81 Mbp_chr2_146805136_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr2 146.81 Mbp_chr2_146805136_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chrX 13.21 Mbp_chrX_13212511_F | TCGTCGGCAGCGTCACCATGGGCCTGGAGTAGAA | 60.2 | 0.26 |
| mm4__chrX 13.21 Mbp_chrX_13212511_R | GTCTCGTGGGCTCGGTCATGCATTTGTAACTCTTGCCT | 58.9 | 0.26 |
| mm4__chr20 60.40 Mbp_chr20_60398552_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr20 60.40 Mbp_chr20_60398552_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr3 149.36 Mbp_chr3_149356062_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr3 149.36 Mbp_chr3_149356062_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr11 44.60 Mbp_chr11_44599835_F | TCGTCGGCAGCGTCCCTGAACTGGGTGGAGTCG | 59.7 | 0.23 |
| mm4__chr11 44.60 Mbp_chr11_44599835_R | GTCTCGTGGGCTCGGGTGATTCAGCCAAGCCTCCT | 60.0 | 0.23 |
| mm4__chr13 42.04 Mbp_chr13_42040332_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr13 42.04 Mbp_chr13_42040332_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr2 236.72 Mbp_chr2_236718798_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr2 236.72 Mbp_chr2_236718798_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr19 3.60 Mbp_chr19_3604767_F | TCGTCGGCAGCGTCCTCCCTGGCTCTCACCTCT | 59.9 | 0.21 |
| mm4__chr19 3.60 Mbp_chr19_3604767_R | GTCTCGTGGGCTCGGCTGTGGGTCCAAGGTCCAAG | 60.2 | 0.21 |
| mm4__chr22 50.01 Mbp_chr22_50005981_F | TCGTCGGCAGCGTCTTGCACCAACTCAACTGCTG | 59.2 | 0.21 |
| mm4__chr22 50.01 Mbp_chr22_50005981_R | GTCTCGTGGGCTCGGCTACGCCCTGCTTTCCTCAG | 60.4 | 0.21 |
| mm4__chr1 38.05 Mbp_chr1_38045206_F | TCGTCGGCAGCGTCTTGGCGAGTTCCGGATCTTC | 60.1 | 0.21 |
| mm4__chr1 38.05 Mbp_chr1_38045206_R | GTCTCGTGGGCTCGGCAAAACCAAACACCCGGACG | 60.2 | 0.21 |
| mm4__chr3 88.10 Mbp_chr3_88096208_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr3 88.10 Mbp_chr3_88096208_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr4 74.82 Mbp_chr4_74821788_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr4 74.82 Mbp_chr4_74821788_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr1 226.64 Mbp_chr1_226637334_F | TCGTCGGCAGCGTCGAAGATGTAGGTGGCGGTCG | 60.5 | 0.17 |
| mm4__chr1 226.64 Mbp_chr1_226637334_R | GTCTCGTGGGCTCGGTGTCCTTCCAGCTCCAGAGT | 60.1 | 0.17 |
| mm4__chr9 74.28 Mbp_chr9_74284775_F | TCGTCGGCAGCGTCGGTTTGCAGTGTCTGGAAGC | 59.6 | 0.17 |
| mm4__chr9 74.28 Mbp_chr9_74284775_R | GTCTCGTGGGCTCGGAGCTGGAGATAGGCCAATGC | 59.8 | 0.17 |
| mm4__chr4 43.34 Mbp_chr4_43344657_F | TCGTCGGCAGCGTCTCCACCACATCCAGTTCTGC | 59.9 | 0.16 |
| mm4__chr4 43.34 Mbp_chr4_43344657_R | GTCTCGTGGGCTCGGGGAGGGTTGCTTGGAAAAGG | 59.0 | 0.16 |
| mm3__chrX 80.24 Mbp_chrX_80238092_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3__chrX 80.24 Mbp_chrX_80238092_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm2__chrX 79.72 Mbp_chrX_79721921_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm2__chrX 79.72 Mbp_chrX_79721921_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr1 56.83 Mbp_chr1_56828300_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr1 56.83 Mbp_chr1_56828300_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr5 123.09 Mbp_chr5_123093819_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr5 123.09 Mbp_chr5_123093819_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr8 141.94 Mbp_chr8_141940164_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr8 141.94 Mbp_chr8_141940164_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr11 2.44 Mbp_chr11_2440825_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr11 2.44 Mbp_chr11_2440825_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 72.97 Mbp_chr3_72966722_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 72.97 Mbp_chr3_72966722_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr4 78.56 Mbp_chr4_78562835_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr4 78.56 Mbp_chr4_78562835_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr14 104.46 Mbp_chr14_104461500_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr14 104.46 Mbp_chr14_104461500_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr10 91.17 Mbp_chr10_91171615_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr10 91.17 Mbp_chr10_91171615_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr3 19.17 Mbp_chr3_19174151_F | TCGTCGGCAGCGTCCTGTGGCAGGTAACATTTTCCC | 59.7 | 0.11 |
| mm4__chr3 19.17 Mbp_chr3_19174151_R | GTCTCGTGGGCTCGGAGGCCTGGAGCTAATGCAAG | 60.1 | 0.11 |
| mm4__chr2 77.78 Mbp_chr2_77779654_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr2 77.78 Mbp_chr2_77779654_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr1 72.69 Mbp_chr1_72693889_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr1 72.69 Mbp_chr1_72693889_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrY 4.15 Mbp_chrY_4153408_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrY 4.15 Mbp_chrY_4153408_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrY 3.86 Mbp_chrY_3859554_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrY 3.86 Mbp_chrY_3859554_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrX 90.58 Mbp_chrX_90575648_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrX 90.58 Mbp_chrX_90575648_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrX 90.28 Mbp_chrX_90282797_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chrX 90.28 Mbp_chrX_90282797_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr7 12.30 Mbp_chr7_12303303_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr7 12.30 Mbp_chr7_12303303_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr14 96.12 Mbp_chr14_96117559_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr14 96.12 Mbp_chr14_96117559_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr1 244.30 Mbp_chr1_244303993_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr1 244.30 Mbp_chr1_244303993_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr4 146.70 Mbp_chr4_146697168_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr4 146.70 Mbp_chr4_146697168_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr1 43.45 Mbp_chr1_43453739_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr1 43.45 Mbp_chr1_43453739_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr14 105.12 Mbp_chr14_105115423_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr14 105.12 Mbp_chr14_105115423_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr16 4.91 Mbp_chr16_4914807_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr16 4.91 Mbp_chr16_4914807_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr10 62.05 Mbp_chr10_62046533_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr10 62.05 Mbp_chr10_62046533_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr3 55.19 Mbp_chr3_55189034_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr3 55.19 Mbp_chr3_55189034_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr1 230.07 Mbp_chr1_230068291_F | TCGTCGGCAGCGTCCCTGGGCATTCGGGAGGAT | 61.4 | 0.08 |
| mm4__chr1 230.07 Mbp_chr1_230068291_R | GTCTCGTGGGCTCGGACACTACAAACGTCCCCTGC | 60.2 | 0.08 |
| mm4__chr15 50.70 Mbp_chr15_50697075_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr15 50.70 Mbp_chr15_50697075_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3__chr19 18.85 Mbp_chr19_18851209_F | TCGTCGGCAGCGTCGTGGTGGGAGGGGAAGAATG | 60.0 | 0.08 |
| mm3__chr19 18.85 Mbp_chr19_18851209_R | GTCTCGTGGGCTCGGTCTGTCTCAGCTGACTGTGC | 59.6 | 0.08 |
| mm4__chr7 149.39 Mbp_chr7_149390927_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr7 149.39 Mbp_chr7_149390927_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr22 49.15 Mbp_chr22_49147576_F | TCGTCGGCAGCGTCGCCCTCAACAAGCAAGAGAA | 58.3 | 0.08 |
| mm4__chr22 49.15 Mbp_chr22_49147576_R | GTCTCGTGGGCTCGGATCTGGGGTCTCGGGAGATG | 60.4 | 0.08 |
| mm4__chrX 98.86 Mbp_chrX_98856436_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 98.86 Mbp_chrX_98856436_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 42.13 Mbp_chr1_42128312_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 42.13 Mbp_chr1_42128312_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 18.54 Mbp_chr1_18535664_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 18.54 Mbp_chr1_18535664_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr10 19.63 Mbp_chr10_19627523_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 19.63 Mbp_chr10_19627523_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr16 49.91 Mbp_chr16_49911037_F | TCGTCGGCAGCGTCCACTAATGAGGGGCCTCTGC | 60.1 | 0.06 |
| mm4__chr16 49.91 Mbp_chr16_49911037_R | GTCTCGTGGGCTCGGCGGCCCTAACTCACTGAAGG | 60.1 | 0.06 |
| mm4__chr19 58.36 Mbp_chr19_58362297_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr19 58.36 Mbp_chr19_58362297_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 27.73 Mbp_chr21_27726528_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 27.73 Mbp_chr21_27726528_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 97.71 Mbp_chr10_97714069_F | TCGTCGGCAGCGTCTCACTATCGCCACCAGCAAG | 60.1 | 0.05 |
| mm4__chr10 97.71 Mbp_chr10_97714069_R | GTCTCGTGGGCTCGGCAGCGTGAGGAAGTAGAGGC | 60.1 | 0.05 |
| mm4__chr10 95.68 Mbp_chr10_95682092_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr10 95.68 Mbp_chr10_95682092_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr1 21.15 Mbp_chr1_21148757_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr1 21.15 Mbp_chr1_21148757_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr2 134.56 Mbp_chr2_134564070_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr2 134.56 Mbp_chr2_134564070_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr16 88.46 Mbp_chr16_88455927_F | TCGTCGGCAGCGTCCCGCTGGGTTTTCCTCCC | 60.3 | 0.05 |
| mm4__chr16 88.46 Mbp_chr16_88455927_R | GTCTCGTGGGCTCGGAGGCCTCTTATCGCTCTTGG | 59.2 | 0.05 |
| mm3__chr3 41.03 Mbp_chr3_41025925_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr3 41.03 Mbp_chr3_41025925_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm2__chr15 49.86 Mbp_chr15_49856998_F | TCGTCGGCAGCGTCCCACAGGGTTTAGCAGCTCT | 59.6 | 0.05 |
| mm2__chr15 49.86 Mbp_chr15_49856998_R | GTCTCGTGGGCTCGGGACTAGCCCCTGATGCACTG | 60.1 | 0.05 |
| mm4__chr3 38.68 Mbp_chr3_38681682_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 38.68 Mbp_chr3_38681682_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 48.66 Mbp_chr3_48664538_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 48.66 Mbp_chr3_48664538_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 14.35 Mbp_chr3_14351564_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr3 14.35 Mbp_chr3_14351564_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr11 50.21 Mbp_chr11_50211487_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr11 50.21 Mbp_chr11_50211487_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr5 3.87 Mbp_chr5_3874172_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr5 3.87 Mbp_chr5_3874172_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm2__chr20 36.97 Mbp_chr20_36970015_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm2__chr20 36.97 Mbp_chr20_36970015_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3__chr12 95.84 Mbp_chr12_95843539_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3__chr12 95.84 Mbp_chr12_95843539_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr19 18.93 Mbp_chr19_18932689_F | TCGTCGGCAGCGTCCGTGGTCAGGGTGGTTATCC | 60.1 | 0.04 |
| mm4__chr19 18.93 Mbp_chr19_18932689_R | GTCTCGTGGGCTCGGCCCCTCTGTTTTGACCCCAT | 59.5 | 0.04 |
| mm4__chr1 25.87 Mbp_chr1_25870209_F | TCGTCGGCAGCGTCTGAGTGGTCAGCCTCTGAGT | 60.1 | 0.04 |
| mm4__chr1 25.87 Mbp_chr1_25870209_R | GTCTCGTGGGCTCGGCCCAGTGCAATTTGCCAGAC | 60.0 | 0.04 |
| mm4__chr7 44.03 Mbp_chr7_44031029_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 44.03 Mbp_chr7_44031029_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.68 Mbp_chr7_102683527_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.68 Mbp_chr7_102683527_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.58 Mbp_chr7_102584466_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.58 Mbp_chr7_102584466_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.49 Mbp_chr7_102485287_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 102.49 Mbp_chr7_102485287_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 46.18 Mbp_chr7_46184897_F | TCGTCGGCAGCGTCCCCTGGTTCTCTGCTCACAG | 60.0 | 0.04 |
| mm4__chr7 46.18 Mbp_chr7_46184897_R | GTCTCGTGGGCTCGGTCCCTCCACCTCTTCTGACC | 60.2 | 0.04 |
| mm4__chr2 198.23 Mbp_chr2_198226954_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr2 198.23 Mbp_chr2_198226954_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr19 30.94 Mbp_chr19_30944510_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr19 30.94 Mbp_chr19_30944510_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 59.94 Mbp_chr17_59944482_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 59.94 Mbp_chr17_59944482_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr12 76.06 Mbp_chr12_76057190_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr12 76.06 Mbp_chr12_76057190_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 121.71 Mbp_chr10_121705866_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 121.71 Mbp_chr10_121705866_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 116.46 Mbp_chr1_116463786_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 116.46 Mbp_chr1_116463786_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr9 35.72 Mbp_chr9_35722723_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr9 35.72 Mbp_chr9_35722723_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 119.79 Mbp_chr1_119789976_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 119.79 Mbp_chr1_119789976_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr10 85.68 Mbp_chr10_85680360_F | TCGTCGGCAGCGTCGCCCAGGAGTCTACAGCTTC | 59.8 | 0.03 |
| mm3__chr10 85.68 Mbp_chr10_85680360_R | GTCTCGTGGGCTCGGCCATCAGGAGTTGGCCATGA | 59.7 | 0.03 |
| mm4__chr5 34.41 Mbp_chr5_34407438_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 34.41 Mbp_chr5_34407438_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 20.80 Mbp_chr5_20796462_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 20.80 Mbp_chr5_20796462_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr13 105.06 Mbp_chr13_105059988_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr13 105.06 Mbp_chr13_105059988_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr1 25.88 Mbp_chr1_25881961_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr1 25.88 Mbp_chr1_25881961_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 1.17 Mbp_chr17_1168724_F | TCGTCGGCAGCGTCTGTCCTTCACTCAGGGAGGG | 60.5 | 0.03 |
| mm4__chr17 1.17 Mbp_chr17_1168724_R | GTCTCGTGGGCTCGGGAACGCACATTGGCTGTCAG | 60.1 | 0.03 |
| mm4__chr20 59.45 Mbp_chr20_59450844_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr20 59.45 Mbp_chr20_59450844_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr20 31.41 Mbp_chr20_31405995_F | TCGTCGGCAGCGTCGTACTGGGCACTGGGAAGAC | 60.0 | 0.03 |
| mm4__chr20 31.41 Mbp_chr20_31405995_R | GTCTCGTGGGCTCGGAGAACTCTGGGGACTCTGACA | 59.8 | 0.03 |
| mm4__chr7 94.57 Mbp_chr7_94565608_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr7 94.57 Mbp_chr7_94565608_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 2.83 Mbp_chr17_2827691_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr17 2.83 Mbp_chr17_2827691_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 7.37 Mbp_chr4_7365433_F | TCGTCGGCAGCGTCCGAGGAGGACCCCTAGGATT | 59.8 | 0.02 |
| mm4__chr4 7.37 Mbp_chr4_7365433_R | GTCTCGTGGGCTCGGAACTCTCGACAGCTCTGGTG | 59.3 | 0.02 |
| mm4__chr14 77.21 Mbp_chr14_77208794_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr14 77.21 Mbp_chr14_77208794_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 147.61 Mbp_chr4_147614121_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 147.61 Mbp_chr4_147614121_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr8 130.60 Mbp_chr8_130596338_F | TCGTCGGCAGCGTCCCCTCTGCCATGGGAAGTTT | 59.9 | 0.02 |
| mm4__chr8 130.60 Mbp_chr8_130596338_R | GTCTCGTGGGCTCGGCCTGCTCAGGTTGGCTGAAT | 60.3 | 0.02 |
| mm4__chr7 39.62 Mbp_chr7_39623965_F | TCGTCGGCAGCGTCAGATTGCACATCCCACGTTCT | 59.9 | 0.02 |
| mm4__chr7 39.62 Mbp_chr7_39623965_R | GTCTCGTGGGCTCGGCAGCCGCTCACTCTCGGG | 62.5 | 0.02 |
| mm4__chr15 100.86 Mbp_chr15_100862341_F | TCGTCGGCAGCGTCACGTGTATCTCTACCACCCC | 58.2 | 0.02 |
| mm4__chr15 100.86 Mbp_chr15_100862341_R | GTCTCGTGGGCTCGGCTCACTCCATTCCTGCCCAG | 60.1 | 0.02 |
| mm4__chr4 16.08 Mbp_chr4_16083984_F | TCGTCGGCAGCGTCGCCGCCGTAATGGAAAGGAT | 60.8 | 0.02 |
| mm4__chr4 16.08 Mbp_chr4_16083984_R | GTCTCGTGGGCTCGGTCCAATCAGAGTGCGTCCAG | 59.7 | 0.02 |
| mm4__chr4 171.67 Mbp_chr4_171669439_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 171.67 Mbp_chr4_171669439_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr14 91.20 Mbp_chr14_91198441_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr14 91.20 Mbp_chr14_91198441_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 81.26 Mbp_chr1_81256908_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 81.26 Mbp_chr1_81256908_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 22.05 Mbp_chr1_22051724_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 22.05 Mbp_chr1_22051724_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 205.63 Mbp_chr1_205629939_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 205.63 Mbp_chr1_205629939_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 8.19 Mbp_chr1_8186135_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 8.19 Mbp_chr1_8186135_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr8 9.13 Mbp_chr8_9130448_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr8 9.13 Mbp_chr8_9130448_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr13 105.82 Mbp_chr13_105824832_F | TCGTCGGCAGCGTCACTGAAAGCTACTAGTGGGCA | 58.7 | 0.01 |
| mm4__chr13 105.82 Mbp_chr13_105824832_R | GTCTCGTGGGCTCGGCACAGCACCAGGAGGAATCA | 59.6 | 0.01 |
| mm4__chr18 79.70 Mbp_chr18_79696057_F | TCGTCGGCAGCGTCTAAGAACGGGAAGCAGCAGG | 60.0 | 0.01 |
| mm4__chr18 79.70 Mbp_chr18_79696057_R | GTCTCGTGGGCTCGGCTCCTCAGAGCACCACGC | 60.1 | 0.01 |
| mm4__chr5 168.36 Mbp_chr5_168356486_F | TCGTCGGCAGCGTCCGGTGTCAGGCAAGAGTCTT | 59.9 | 0.01 |
| mm4__chr5 168.36 Mbp_chr5_168356486_R | GTCTCGTGGGCTCGGCAGATCAGAGCAGGGTGTGG | 60.1 | 0.01 |
| mm4__chr6 134.40 Mbp_chr6_134399453_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr6 134.40 Mbp_chr6_134399453_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 24.53 Mbp_chr22_24529775_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 24.53 Mbp_chr22_24529775_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 225.73 Mbp_chr2_225732085_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 225.73 Mbp_chr2_225732085_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 105.78 Mbp_chr14_105775004_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 105.78 Mbp_chr14_105775004_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr22 41.62 Mbp_chr22_41623817_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr22 41.62 Mbp_chr22_41623817_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 188.73 Mbp_chr1_188729823_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 188.73 Mbp_chr1_188729823_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr5 17.46 Mbp_chr5_17457916_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr5 17.46 Mbp_chr5_17457916_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 101.73 Mbp_chr14_101728528_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 101.73 Mbp_chr14_101728528_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr15 41.93 Mbp_chr15_41932305_F | TCGTCGGCAGCGTCGCCAGGGCTCCACGTGAG | 62.7 | 0.01 |
| mm4__chr15 41.93 Mbp_chr15_41932305_R | GTCTCGTGGGCTCGGGTTCCTCTCCCAGAGCAGAC | 59.4 | 0.01 |
| mm4__chr11 45.65 Mbp_chr11_45651486_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr11 45.65 Mbp_chr11_45651486_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr13 93.68 Mbp_chr13_93682915_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr13 93.68 Mbp_chr13_93682915_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 3.22 Mbp_chr19_3219748_F | TCGTCGGCAGCGTCGCTCACAGCCTCGCTATTCT | 59.8 | 0.01 |
| mm4__chr19 3.22 Mbp_chr19_3219748_R | GTCTCGTGGGCTCGGCCAGCATTCTGCCTCCTGAA | 60.0 | 0.01 |
| mm4__chr19 19.51 Mbp_chr19_19512158_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 19.51 Mbp_chr19_19512158_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 18.29 Mbp_chr19_18287726_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 18.29 Mbp_chr19_18287726_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr8 139.98 Mbp_chr8_139976918_F | TCGTCGGCAGCGTCGATGCTGCCTGTTCACCTCT | 60.0 | 0.00 |
| mm4__chr8 139.98 Mbp_chr8_139976918_R | GTCTCGTGGGCTCGGGCCCTGCAGGATTGGAAACT | 60.6 | 0.00 |
| mm3__chr6 111.47 Mbp_chr6_111469059_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr6 111.47 Mbp_chr6_111469059_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr20 11.08 Mbp_chr20_11075400_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr20 11.08 Mbp_chr20_11075400_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr15 86.57 Mbp_chr15_86569194_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr15 86.57 Mbp_chr15_86569194_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr11 3.73 Mbp_chr11_3725917_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr11 3.73 Mbp_chr11_3725917_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 32.99 Mbp_chr1_32995000_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 32.99 Mbp_chr1_32995000_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 109.58 Mbp_chr1_109583446_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 109.58 Mbp_chr1_109583446_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr9 26.71 Mbp_chr9_26709992_F | TCGTCGGCAGCGTCGCCCTACTGACACTTTTGCA | 58.3 | 0.00 |
| mm4__chr9 26.71 Mbp_chr9_26709992_R | GTCTCGTGGGCTCGGTCGTTTGTGGTGGTGGAACT | 59.7 | 0.00 |
| mm4__chr21 31.76 Mbp_chr21_31756523_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 31.76 Mbp_chr21_31756523_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 5.48 Mbp_chr7_5478545_F | TCGTCGGCAGCGTCCTGGTGACGTGGAGCAGG | 60.0 | 0.00 |
| mm4__chr7 5.48 Mbp_chr7_5478545_R | GTCTCGTGGGCTCGGCAACCTCTCTGGCTGCCC | 60.0 | 0.00 |
| mm3__chr7 99.52 Mbp_chr7_99522570_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr7 99.52 Mbp_chr7_99522570_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr7 129.17 Mbp_chr7_129165807_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr7 129.17 Mbp_chr7_129165807_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr17 28.44 Mbp_chr17_28436034_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr17 28.44 Mbp_chr17_28436034_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr13 95.32 Mbp_chr13_95316062_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr13 95.32 Mbp_chr13_95316062_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr12 38.15 Mbp_chr12_38148718_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr12 38.15 Mbp_chr12_38148718_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr21 13.24 Mbp_chr21_13244114_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr21 13.24 Mbp_chr21_13244114_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 124.93 Mbp_chr7_124930045_F | TCGTCGGCAGCGTCCTGACAGGATGCGACGTCAA | 60.3 | 0.00 |
| mm4__chr7 124.93 Mbp_chr7_124930045_R | GTCTCGTGGGCTCGGAAGCTGGAAAGTCACTGGGG | 59.8 | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529657 |
AGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG |
| mm3__chr1 107.27 Mbp_chr1_107273211 |
ATCAGGCCTTGCTAGATGGCATGAATTACTCAGAAGCTTACAGACATCAAGGTAGGGGGGTCCTGAAAGGTGTGGCAGGG GTCGCTGAGCAGTGAGAATAGCCGGGAAGTGAGTGCTGAGTGATGCAGTGGAGA |
| mm2__chr1 981.62 Kbp_chr1_981622 |
GATGACCTTCCGATCGCCTTGGCTGGGCCAGGCTATTCTCTGAGCTCACCAGGTGGTGCTGGCCAGAGCTGGCCCAAGTC AGCACGGAGAG |
| mm4__chr19 36.42 Mbp_chr19_36417593 |
CGCTCGGACCCCTTTTCTTAAAGAATTGGGCAGGACTGGCTAATAAGGGAACCAGGCTTTTCTTGCAGCCCAGCCTCGCA TAGGGCACAATGCCCCCACCGCCCACCCACACATAACCAGA |
| mm4__chr12 22.85 Mbp_chr12_22850013 |
CTGCTGACGGTGGGTACATTTTAGAGGTGAGATGTAAGGATAGCCTGGAGATTTTCACATTTAGATTTCATAGAGTGAAG GGCAAAGGTTAGGACACTTCTTAAGCAACCCAAGAGTCTGAGGA |
| mm4__chr10 132.27 Mbp_chr10_132271362 |
CCCCATCTGTAAAGCAGGCAGCCCTACAGCAACGGTTCCCAGGCCCCTGGCACTCAGGAAAAAACCAGCACACCTGGCAA GAGACCCAGGCAGAGAGCTGCAAGAATAGCATGGAGGGTCAGAACAGAGGCCACGTTC |
| mm4__chr6 51.62 Mbp_chr6_51620531 |
ACTTGTCCTAAACCCTGGGGGGTAGATGGTAATACTGTCATCTACTTTTACTACGTTTACTGTCCCTGCTTTTCTCACAT CTCACCACCAAATACATTAACCACGCATTTGTATGTATTGAAGA |
| mm4__chr19 9.27 Mbp_chr19_9268051 |
AGAAATGGAGCCAAGACCCTGATTTTATGTCTCCTTTGAGGAGGTGGAGAGCTGCAAGGAAAGCCAGGAACGGGGGAAAG GGAGAGATGCAC |
| mm4__chr17 33.31 Mbp_chr17_33310535 |
TGCATTGACGAGGACAAAGGTTAGGGACTGTGGTGCTGATCTGAGGGAATAGCCTGGACTTTGATCCTTAGGCTCAGAAC CAAGTAAAAAATGCCTCTGAAGAAGTTGGACACTGACTGTGCAAGGGTTTGATCCT |
| mm4__chr14 105.25 Mbp_chr14_105253314 |
GGGAGGACACTTCAGAGCAATGTCTGCTGGCAGGTGTGGATGAGGTGCACAGCGGGAATAGCCTGGTCCCTACTGGAGCC TGCAAGAGCCCTGCCCACACTTGGCTGTCTTCATCCCAGGCTT |
| mm4__chrX 13.21 Mbp_chrX_13212511 |
ACCATGGGCCTGGAGTAGAAGGACAGGAAGGAAGGGGAAGCTGAGAGAACAGCCAGGAAGCTGGGACTAACAGCTGTTCT ATTTTTCTGAACAGGGGGAATAAAATTAGGCAAGAGTTACAAATGCATGA |
| mm4__chr11 44.60 Mbp_chr11_44599835 |
CCTGAACTGGGTGGAGTCGGGGGGATGAGCTGAGAAAAGAGCCAGGAGATGTGGCAGGAAGGGTGCCCCTGGCAGGAGGC TGCACCAGGAGGCTTGGCTGAATCAC |
| mm4__chr19 3.60 Mbp_chr19_3604767 |
CTCCCTGGCTCTCACCTCTGCAGGGCTCCTGGCTGTACTCACAGCTCAACTCCCTACAAGGTGCCCGGGCAGCCAGCAGG GAGCCCTGAGGCTCCGTGTCACCCCTGTCGGCCTTGGACCTTGGACCCACAG |
| mm4__chr22 50.01 Mbp_chr22_50005981 |
TTGCACCAACTCAACTGCTGAAAAGTACCAATTGGGCTGGAGAAAAAAACATATGTTTTTTTAAAAACCTTCCTAGAAAC GTAGGAGAGCTGCGAAAATAATCAGGATGGGGGTGCTGAGGAAAGCAGGGCGTAG |
| mm4__chr1 38.05 Mbp_chr1_38045206 |
TTGGCGAGTTCCGGATCTTCGCTCCTCTCTCCCGGGGCTCGGGCGACGCAGCCTACCGGGCTGTCTTCGCCGCTCACCGG AGAGGGTCGTCCGGGTGTTTGGTTTTG |
| mm4__chr1 226.64 Mbp_chr1_226637334 |
GAAGATGTAGGTGGCGGTCGGCGGGTGCCTGGCTACTCTAGCAGCTCAGAGAGCTTCTGCCCTGAGACCACGGGTGTGTG GGATATGAGTCCTGCCACCACTGCCCAGAATGGACAAGACTCTGGAGCTGGAAGGACA |
| mm4__chr9 74.28 Mbp_chr9_74284775 |
GGTTTGCAGTGTCTGGAAGCTGGAAGGGGCTGTGCCCAGGCTATTCCAGCAGCTTAGCTATTGCATTGGCCTATCTCCAG CT |
| mm4__chr4 43.34 Mbp_chr4_43344657 |
TCCACCACATCCAGTTCTGCAGTATCCTTCCGATGTCATTTGTCTGTAGTTCATCAGCCCCCGGCTTTTCTCCCAGCTCA AGATCCTTTTCCAAGCAACCCTCC |
| mm4__chr3 19.17 Mbp_chr3_19174151 |
CTGTGGCAGGTAACATTTTCCCCCTTGCTCTTCCCACAGCTCACCACTAGGCCCCATATCTACAGAAATGGATTTCTTCC CTTTTTTCAGTAATGTTCTGGCACCTCTACTTCTTGCATTAGCTCCAGGCCT |
| mm4__chr1 230.07 Mbp_chr1_230068291 |
CCTGGGCATTCGGGAGGATGGTGAGCGCCGAGAGGAGCCGGGACAGCGCGGTCCTGCGGCCGAGCGCCGGCCGGGAGAGC AGGGCGTCGGGCTGTGTGCTGGAGCATGCCGGCAGGGGACGTTTGTAGTGT |
| mm3__chr19 18.85 Mbp_chr19_18851209 |
GTGGTGGGAGGGGAAGAATGGGGCTGGCTGTGGGCAGACTTGTCCTGCAGAGCCTGAACCTGCCCAGACAGGTGGGCTGC GAGAATGTCCTGGGGAAATAGGTTGGTGTCGAGGTCGGCACAGTCAGCTGAGACAGA |
| mm4__chr22 49.15 Mbp_chr22_49147576 |
GCCCTCAACAAGCAAGAGAACATGACAAATTCTTGCTACCATAGTAACTAAGACGGTTGGAAGGATGAGCTGGGGGAAGA GCCTGGAGGACCAGTGCAATCGTCCGCGGCCTCATCTCCCGAGACCCCAGAT |
| mm4__chr16 49.91 Mbp_chr16_49911037 |
CACTAATGAGGGGCCTCTGCTCCAAGGTGAGCTGAGTGAGGAGCCGGGGTCCCTGCTGGATTCGGAGGAGCTCCCGACAG CTGCTCCCCCTTCAGTGAGTTAGGGCCG |
| mm4__chr10 97.71 Mbp_chr10_97714069 |
TCACTATCGCCACCAGCAAGTACCAGGGCCCCGTGCACTTCGCGCTCTTCGTGTCCGTGCTCTTCTGGCTGCTCACCCTG GGCCTCTACTTCCTCACGCTG |
| mm4__chr16 88.46 Mbp_chr16_88455927 |
CCGCTGGGTTTTCCTCCCGCCCGAGTCGATGTGAGCTCCGATAAGAGCCTGGCCGGTCGGCCCGATGGCGATCGCCAAGA GCGATAAGAGGCCT |
| mm2__chr15 49.86 Mbp_chr15_49856998 | CCACAGGGTTTAGCAGCTCTGTGGTGAGCTGAGAGAAAAGCCAGAGACAGCAGTGCATCAGGGGCTAGTC |
| mm4__chr19 18.93 Mbp_chr19_18932689 |
CGTGGTCAGGGTGGTTATCCTAAGACCAGAGAGGCATCGACGGGGCGAGGTTAGCAGCGAGAAGAGACAGGGATGGGGTC AAAACAGAGGGG |
| mm4__chr1 25.87 Mbp_chr1_25870209 |
TGAGTGGTCAGCCTCTGAGTCGTGTGCCCACCAGGCTGCTGGGCCCTGTCTATTCAGGCAGCTCAGCCCAGCCCACCTCC ATGGGCCCCTCCAAGTCTGGCAAATTGCACTGGG |
| mm4__chr7 46.18 Mbp_chr7_46184897 |
CCCTGGTTCTCTGCTCACAGTCTTGCTGCAGCACTTGGTGAGCTGGGTGAGTACCCAGGAGTTGAGGGTCAGAAGAGGTG GAGGGA |
| mm3__chr10 85.68 Mbp_chr10_85680360 |
GCCCAGGAGTCTACAGCTTCCTTCTCCAGCCTATTCACGCAGCTCATCTTCCCAAAGTGCCACATGATCATGGCCAACTC CTGATGG |
| mm4__chr17 1.17 Mbp_chr17_1168724 |
TGTCCTTCACTCAGGGAGGGGCTTGAGGACAGACACGGCAGGCACCCACAACAAGGGCCTGGCTGTGCTGGCAGCTGACC AGCTGCAGCAAGGTCCTCCGGCTAGAAGCAGCAGCTCTCCTGACAGCCAATGTGCGTTC |
| mm4__chr20 31.41 Mbp_chr20_31405995 |
GTACTGGGCACTGGGAAGACCATTTGGTACCAGAGAACTCAAACTTCCCCATAACTAGAGAGACTACCTAAAGGCCTCAA TGAGCTCTACCAGGCCATTCTCCCAGCCCAACCTGTCAGAGTCCCCAGAGTTCT |
| mm4__chr4 7.37 Mbp_chr4_7365433 |
CGAGGAGGACCCCTAGGATTTTGTTTGGGGCACCAGGAGGGTTGGGGTGCAGGCTGAGATGGGGAGCTGGGACAAGAGCC TGGTCTGGGGTGTCCACCAGAGCTGTCGAGAGTT |
| mm4__chr8 130.60 Mbp_chr8_130596338 |
CCCTCTGCCATGGGAAGTTTCCTGCTGTTGAGAGTTAGCCCTTCTGCCAGCAGCTCTTGGTTCCCAAGCAATTCTCACAG CTCAGCACAAAATGTGTCACTGAGGCAGAGATTCAGCCAACCTGAGCAGG |
| mm4__chr7 39.62 Mbp_chr7_39623965 |
AGATTGCACATCCCACGTTCTGCCCGGGGACGAGCTGCGAGATGAGCCCGGCTGGAATCCGGGGCACCGCGTTCGCCGCT GTGGGGCGAGTGAGCCCCCGAGAGTGAGCGGCTG |
| mm4__chr15 100.86 Mbp_chr15_100862341 |
ACGTGTATCTCTACCACCCCCACTACATATTCTGAAGAACCAAGGTGAGCTGGGGGAATCCCCAGGCCAGCAGGGCAAGG CCTGGGCAGGAATGGAGTGAG |
| mm4__chr4 16.08 Mbp_chr4_16083984 |
GCCGCCGTAATGGAAAGGATTCCTTAAACATACTCACCGCGGCGGGAGAGCTGAGAGCATGGCCAGGTGCCGCGTGGGGA TCTGCCTCAGTCACTTAAAGATCGCGAAACCCGAGCCCTGGACGCACTCTGATTGGA |
| mm4__chr13 105.82 Mbp_chr13_105824832 |
ACTGAAAGCTACTAGTGGGCAAAGATTGTGTTTATTAAGTAGTCACTTACTTTAGATGACCGATTAGCTGCCAGAATTGC CTGGCTCTACCGTGCAAAAATAACATGCTGTATTTGATTCCTCCTGGTGCTGTG |
| mm4__chr18 79.70 Mbp_chr18_79696057 |
TAAGAACGGGAAGCAGCAGGTGCCGCTGTCCACGGCGACCGTGTCCATGGTGCACAGCGTGCCGGAGTTGATGGTGAGCT CCGAGGTGAGCCGGGCATCAGTGGCGGCGTGTTGGGGAAGCGTGGTGCTCTGAGGAG |
| mm4__chr5 168.36 Mbp_chr5_168356486 | CGGTGTCAGGCAAGAGTCTTCCAAGTCAGCTGAGCTGGGAGAATTCCCCGGCCCACACCCTGCTCTGATCTG |
| mm4__chr15 41.93 Mbp_chr15_41932305 |
GCCAGGGCTCCACGTGAGTGGGCGGGTGAGCTGGGAGAAGCCCCAGGGTCTGCAGGCAGGGTCTGCTCTGGGAGAGGAAC |
| mm4__chr19 3.22 Mbp_chr19_3219748 |
GCTCACAGCCTCGCTATTCTCCAGGCTCACAGCCTCGCTATTCTCCGTGCTCACCCACCCCCTTAGCTGTGCCACGGATG AAATTCAGGAGGCAGAATGCTGG |
| mm4__chr8 139.98 Mbp_chr8_139976918 |
GATGCTGCCTGTTCACCTCTGCTCCTCTGAGAGGAGCAGTGTGGGGGAGCTGTAGGGGGAGACACAGGGTCCCCGAGACA AACTGGTGAGCTGCCAGAAGTGTCTGGAAGTTTCCAATCCTGCAGGGC |
| mm4__chr9 26.71 Mbp_chr9_26709992 |
GCCCTACTGACACTTTTGCAATTATCAATACCACCACTACCACTTTCTCCATCACTTCCTCACTTCCAATCCCAGCTCTG CTCCCAGCTCACCATCTTCACCTCTAAAACCTCCATTACTAGTTCCACCACCACAAACGA |
| mm4__chr7 5.48 Mbp_chr7_5478545 |
CTGGTGACGTGGAGCAGGAGGCGGGGCGGAGGCTGCGGGACACGGGGCTGCAGGGCCGGGAAGGGGGTCAGGAGAATAAA TAGCAGCTAGGTGTGGGGTGAGCTGGGAGCACAGGCAGGAGGGGGCAGCCAGAGAGGTTG |
| mm4__chr7 124.93 Mbp_chr7_124930045 |
CTGACAGGATGCGACGTCAACGGGAGCGCAGGGCCAAGGTACAGGCCCCGCCCAGTCCGGTCACATCCCCCGGAATTCTC GCAGCTCCCCTTAGCTCCGCCCCCAGTGACTTTCCAGCTT |