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Contents:

PCR primers for off-targets of AGCCGAGGTCGCCCCCGCCC TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529689_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529689_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr17 32.97 Mbp_chr17_32974935_F Primer3: not found at this Tm N.d. 0.70
mm4__chr17 32.97 Mbp_chr17_32974935_R Primer3: not found at this Tm N.d. 0.70
mm4__chr19 11.35 Mbp_chr19_11349188_F Primer3: not found at this Tm N.d. 0.64
mm4__chr19 11.35 Mbp_chr19_11349188_R Primer3: not found at this Tm N.d. 0.64
mm4__chr4 144.65 Mbp_chr4_144645852_F TCGTCGGCAGCGTCGGGCAGTCCCGGAATCTC 59.4 0.61
mm4__chr4 144.65 Mbp_chr4_144645852_R GTCTCGTGGGCTCGGCTTCGGAACCAGCCTCGAG 60.1 0.61
mm4__chr2 216.37 Mbp_chr2_216371524_F Primer3: not found at this Tm N.d. 0.57
mm4__chr2 216.37 Mbp_chr2_216371524_R Primer3: not found at this Tm N.d. 0.57
mm4__chr14 105.71 Mbp_chr14_105709589_F Primer3: not found at this Tm N.d. 0.56
mm4__chr14 105.71 Mbp_chr14_105709589_R Primer3: not found at this Tm N.d. 0.56
mm4__chr1 2.57 Mbp_chr1_2569571_F TCGTCGGCAGCGTCGTTTCCCTCCTGCCACGAAA 60.5 0.56
mm4__chr1 2.57 Mbp_chr1_2569571_R GTCTCGTGGGCTCGGCATCCTCAATGGGCCTCC 57.0 0.56
mm4__chr1 2.55 Mbp_chr1_2552069_F Primer3: not found at this Tm N.d. 0.56
mm4__chr1 2.55 Mbp_chr1_2552069_R Primer3: not found at this Tm N.d. 0.56
mm4__chr5 9.06 Mbp_chr5_9061680_F Primer3: not found at this Tm N.d. 0.53
mm4__chr5 9.06 Mbp_chr5_9061680_R Primer3: not found at this Tm N.d. 0.53
mm3__chr15 90.72 Mbp_chr15_90717639_F TCGTCGGCAGCGTCTCCTGGGAGGACAGCAGATA 59.3 0.52
mm3__chr15 90.72 Mbp_chr15_90717639_R GTCTCGTGGGCTCGGACAACATGCCAGGTCCTAGC 60.0 0.52
mm4__chr14 104.16 Mbp_chr14_104157867_F TCGTCGGCAGCGTCCCCTGATGGCTTGTCGAAGG 60.7 0.52
mm4__chr14 104.16 Mbp_chr14_104157867_R GTCTCGTGGGCTCGGATGAAGAAGGAGGCTGCAGC 60.3 0.52
mm4__chr19 18.65 Mbp_chr19_18650182_F TCGTCGGCAGCGTCTTCCACAGGGGTCAAAGACG 59.8 0.50
mm4__chr19 18.65 Mbp_chr19_18650182_R GTCTCGTGGGCTCGGCATCCCGTGGAGAGCAGAAC 60.4 0.50
mm4__chr17 36.88 Mbp_chr17_36884812_F TCGTCGGCAGCGTCATTGAGCACAGACCAGGCTC 60.0 0.49
mm4__chr17 36.88 Mbp_chr17_36884812_R GTCTCGTGGGCTCGGCTGTCTCCATCTGCACCCAG 60.1 0.49
mm3__chr17 28.81 Mbp_chr17_28812288_F TCGTCGGCAGCGTCGCCCTGGAAAGGTGAGGTT 59.5 0.48
mm3__chr17 28.81 Mbp_chr17_28812288_R GTCTCGTGGGCTCGGCTGCCTAGACCGCTAGAAGC 59.9 0.48
mm4__chr19 2.56 Mbp_chr19_2556800_F Primer3: not found at this Tm N.d. 0.48
mm4__chr19 2.56 Mbp_chr19_2556800_R Primer3: not found at this Tm N.d. 0.48
mm4__chr16 1.63 Mbp_chr16_1627581_F TCGTCGGCAGCGTCTTGAGTACGGTGCATGCTGT 59.9 0.43
mm4__chr16 1.63 Mbp_chr16_1627581_R GTCTCGTGGGCTCGGAGAATCAAGCCACACTGCCA 59.8 0.43
mm2__chr17 76.24 Mbp_chr17_76239976_F TCGTCGGCAGCGTCCCTCGGTGACCCTGCTAGA 60.3 0.38
mm2__chr17 76.24 Mbp_chr17_76239976_R GTCTCGTGGGCTCGGCTTCGGGGACTCAGTGGTTG 60.3 0.38
mm4__chr15 22.98 Mbp_chr15_22980402_F Primer3: not found at this Tm N.d. 0.38
mm4__chr15 22.98 Mbp_chr15_22980402_R Primer3: not found at this Tm N.d. 0.38
mm4__chr19 54.14 Mbp_chr19_54141270_F TCGTCGGCAGCGTCCAGGTAGGGCGTGATAGCTG 59.9 0.36
mm4__chr19 54.14 Mbp_chr19_54141270_R GTCTCGTGGGCTCGGTCCTCAGTAAAAGGCCCAACC 59.9 0.36
mm4__chr9 99.22 Mbp_chr9_99221638_F TCGTCGGCAGCGTCGAAAACCATGCGCACGTAGG 60.1 0.36
mm4__chr9 99.22 Mbp_chr9_99221638_R GTCTCGTGGGCTCGGACTGTTTCGCCGAGAGCC 60.0 0.36
mm4__chr17 81.39 Mbp_chr17_81385048_F TCGTCGGCAGCGTCCAACCTCAGCCAAGGGTCTC 60.3 0.35
mm4__chr17 81.39 Mbp_chr17_81385048_R GTCTCGTGGGCTCGGCTCATTGCTGGGGAGGTCTC 59.8 0.35
mm4__chr14 105.71 Mbp_chr14_105710581_F Primer3: not found at this Tm N.d. 0.34
mm4__chr14 105.71 Mbp_chr14_105710581_R Primer3: not found at this Tm N.d. 0.34
mm4__chr1 231.04 Mbp_chr1_231040390_F TCGTCGGCAGCGTCCTCTCCTCGAGGCCCCTG 60.8 0.30
mm4__chr1 231.04 Mbp_chr1_231040390_R GTCTCGTGGGCTCGGCACGCGGAGTTGGAGATGAA 60.3 0.30
mm4__chr19 58.40 Mbp_chr19_58396564_F Primer3: not found at this Tm N.d. 0.28
mm4__chr19 58.40 Mbp_chr19_58396564_R Primer3: not found at this Tm N.d. 0.28
mm4__chr13 110.12 Mbp_chr13_110123393_F Primer3: not found at this Tm N.d. 0.28
mm4__chr13 110.12 Mbp_chr13_110123393_R Primer3: not found at this Tm N.d. 0.28
mm4__chr19 58.56 Mbp_chr19_58563230_F TCGTCGGCAGCGTCACCTTGCCACATACATCGCA 60.0 0.28
mm4__chr19 58.56 Mbp_chr19_58563230_R GTCTCGTGGGCTCGGCTCTGATCACCACCCGCTG 60.1 0.28
mm4__chr8 58.76 Mbp_chr8_58755347_F Primer3: not found at this Tm N.d. 0.28
mm4__chr8 58.76 Mbp_chr8_58755347_R Primer3: not found at this Tm N.d. 0.28
mm4__chr11 550.01 Kbp_chr11_550012_F TCGTCGGCAGCGTCCGCCTCTTTGCCAGCACTTA 60.9 0.27
mm4__chr11 550.01 Kbp_chr11_550012_R GTCTCGTGGGCTCGGGCTCAGGTCCGAGATGTTGT 59.7 0.27
mm4__chr2 38.60 Mbp_chr2_38602463_F TCGTCGGCAGCGTCTGTTACCGGCTGAGAGAAGC 59.7 0.26
mm4__chr2 38.60 Mbp_chr2_38602463_R GTCTCGTGGGCTCGGCAAGCGTCTCAAGACCGAGG 60.7 0.26
mm4__chr10 101.84 Mbp_chr10_101840838_F TCGTCGGCAGCGTCTGACCTCCTAACTCTGGGCA 59.8 0.26
mm4__chr10 101.84 Mbp_chr10_101840838_R GTCTCGTGGGCTCGGTCCGGCCCTGAGTATTGTTG 59.7 0.26
mm4__chr7 987.17 Kbp_chr7_987173_F TCGTCGGCAGCGTCCACCCTGGACATGGTCATGG 60.3 0.25
mm4__chr7 987.17 Kbp_chr7_987173_R GTCTCGTGGGCTCGGATCAGAAGTGCGGCCCAC 60.0 0.25
mm4__chr15 55.99 Mbp_chr15_55993577_F TCGTCGGCAGCGTCTTCCGAACGCTTCCAGCAAA 60.8 0.24
mm4__chr15 55.99 Mbp_chr15_55993577_R GTCTCGTGGGCTCGGCTGCTGTCGCTGAGGGAG 59.8 0.24
mm4__chr4 1.80 Mbp_chr4_1803147_F Primer3: not found at this Tm N.d. 0.24
mm4__chr4 1.80 Mbp_chr4_1803147_R Primer3: not found at this Tm N.d. 0.24
mm4__chr16 75.29 Mbp_chr16_75294979_F TCGTCGGCAGCGTCTCCTCACCATAAAGCGTGCT 59.3 0.23
mm4__chr16 75.29 Mbp_chr16_75294979_R GTCTCGTGGGCTCGGGCAGAGATTCAATTTTGTCTCCCA 59.5 0.23
mm4__chr19 9.91 Mbp_chr19_9913150_F TCGTCGGCAGCGTCCCCGGACTCAGCTCAATTCT 59.4 0.23
mm4__chr19 9.91 Mbp_chr19_9913150_R GTCTCGTGGGCTCGGCGAATAGCGTACCAGGCTCG 60.6 0.23
mm3__chr11 112.96 Mbp_chr11_112963576_F TCGTCGGCAGCGTCCAAGCCTAGTCCGCCTGG 59.8 0.23
mm3__chr11 112.96 Mbp_chr11_112963576_R GTCTCGTGGGCTCGGGCCCCATGTGGACAGGTC 60.0 0.23
mm4__chr1 40.78 Mbp_chr1_40784030_F Primer3: not found at this Tm N.d. 0.22
mm4__chr1 40.78 Mbp_chr1_40784030_R Primer3: not found at this Tm N.d. 0.22
mm4__chr20 45.89 Mbp_chr20_45891642_F Primer3: not found at this Tm N.d. 0.21
mm4__chr20 45.89 Mbp_chr20_45891642_R Primer3: not found at this Tm N.d. 0.21
mm4__chrY 257.58 Kbp_chrY_257583_F Primer3: not found at this Tm N.d. 0.21
mm4__chrY 257.58 Kbp_chrY_257583_R Primer3: not found at this Tm N.d. 0.21
mm4__chr6 30.78 Mbp_chr6_30783770_F Primer3: not found at this Tm N.d. 0.21
mm4__chr6 30.78 Mbp_chr6_30783770_R Primer3: not found at this Tm N.d. 0.21
mm4__chr5 79.56 Mbp_chr5_79556938_F Primer3: not found at this Tm N.d. 0.21
mm4__chr5 79.56 Mbp_chr5_79556938_R Primer3: not found at this Tm N.d. 0.21
mm4__chr16 30.81 Mbp_chr16_30807134_F Primer3: not found at this Tm N.d. 0.21
mm4__chr16 30.81 Mbp_chr16_30807134_R Primer3: not found at this Tm N.d. 0.21
mm4__chr11 69.70 Mbp_chr11_69702376_F TCGTCGGCAGCGTCCCTCTGCATCCTTTCTGGGG 60.1 0.21
mm4__chr11 69.70 Mbp_chr11_69702376_R GTCTCGTGGGCTCGGAGGAGGCTGTGTGAGGTCTC 60.9 0.21
mm4__chr11 124.67 Mbp_chr11_124673768_F TCGTCGGCAGCGTCCGCCTAGGACTGGGAAAGTG 60.1 0.20
mm4__chr11 124.67 Mbp_chr11_124673768_R GTCTCGTGGGCTCGGAGTACAGCCCAGTCCTGAGG 60.6 0.20
mm4__chr17 18.26 Mbp_chr17_18264127_F Primer3: not found at this Tm N.d. 0.19
mm4__chr17 18.26 Mbp_chr17_18264127_R Primer3: not found at this Tm N.d. 0.19
mm4__chr14 95.39 Mbp_chr14_95389786_F TCGTCGGCAGCGTCGCACGGGTCACAGAGTCTTT 60.2 0.19
mm4__chr14 95.39 Mbp_chr14_95389786_R GTCTCGTGGGCTCGGATTCATTGCCCAGGCTTCCA 59.9 0.19
mm4__chr11 45.41 Mbp_chr11_45407356_F TCGTCGGCAGCGTCCATTCTTGTTGGCCGTCTGC 60.1 0.18
mm4__chr11 45.41 Mbp_chr11_45407356_R GTCTCGTGGGCTCGGCCAAGGGTCTGAGGATGCTG 60.1 0.18
mm4__chr11 19.89 Mbp_chr11_19887891_F TCGTCGGCAGCGTCTGTAATCATCTCGCCTGGCA 59.1 0.18
mm4__chr11 19.89 Mbp_chr11_19887891_R GTCTCGTGGGCTCGGAAACCACTTCAGAGGCTGGG 59.8 0.18
mm4__chr17 4.59 Mbp_chr17_4593372_F TCGTCGGCAGCGTCTGCCCAGAGATGCTGTGAAA 59.5 0.18
mm4__chr17 4.59 Mbp_chr17_4593372_R GTCTCGTGGGCTCGGAAGAGGCCACCTTCCAACTG 59.8 0.18
mm4__chr8 141.18 Mbp_chr8_141183873_F Primer3: not found at this Tm N.d. 0.17
mm4__chr8 141.18 Mbp_chr8_141183873_R Primer3: not found at this Tm N.d. 0.17
mm4__chr5 139.27 Mbp_chr5_139274069_F TCGTCGGCAGCGTCCGAAGCCACGCCCAGTAG 60.5 0.17
mm4__chr5 139.27 Mbp_chr5_139274069_R GTCTCGTGGGCTCGGCTCTGGGTCCTTACTCACGC 59.8 0.17
mm4__chr2 70.30 Mbp_chr2_70301673_F TCGTCGGCAGCGTCTAAGAGGGGAAGGTGGTGGG 60.5 0.17
mm4__chr2 70.30 Mbp_chr2_70301673_R GTCTCGTGGGCTCGGTGGCGGATCGGATGAAATCC 60.2 0.17
mm4__chr19 402.17 Kbp_chr19_402174_F TCGTCGGCAGCGTCTAATCGAATCCGGCACTGGG 59.8 0.16
mm4__chr19 402.17 Kbp_chr19_402174_R GTCTCGTGGGCTCGGCGGAAGCCGGCACAAAATAG 59.9 0.16
mm4__chr19 11.02 Mbp_chr19_11019849_F Primer3: not found at this Tm N.d. 0.15
mm4__chr19 11.02 Mbp_chr19_11019849_R Primer3: not found at this Tm N.d. 0.15
mm4__chr3 49.43 Mbp_chr3_49429053_F TCGTCGGCAGCGTCCGGCAGAGGCTTTCCCATAA 60.1 0.15
mm4__chr3 49.43 Mbp_chr3_49429053_R GTCTCGTGGGCTCGGCTCCCTTCGAGGTGAGCAAG 60.1 0.15
mm4__chr17 50.20 Mbp_chr17_50200698_F TCGTCGGCAGCGTCGCAACGCTAAGCAATGGGG 59.8 0.15
mm4__chr17 50.20 Mbp_chr17_50200698_R GTCTCGTGGGCTCGGAAGTAAAGCCAGGGATGGGG 59.3 0.15
mm4__chr11 126.41 Mbp_chr11_126406180_F TCGTCGGCAGCGTCTGCCTGCTGAGAAACATGGT 59.8 0.14
mm4__chr11 126.41 Mbp_chr11_126406180_R GTCTCGTGGGCTCGGCAGGATCCTGCTGAAGACGG 60.1 0.14
mm4__chr7 101.82 Mbp_chr7_101816451_F Primer3: not found at this Tm N.d. 0.14
mm4__chr7 101.82 Mbp_chr7_101816451_R Primer3: not found at this Tm N.d. 0.14
mm4__chr4 78.94 Mbp_chr4_78939293_F TCGTCGGCAGCGTCTTCTGATGCATCGTTCCCGG 60.4 0.13
mm4__chr4 78.94 Mbp_chr4_78939293_R GTCTCGTGGGCTCGGCGGTAGGACCTCGCGCAG 62.5 0.13
mm4__chr15 67.49 Mbp_chr15_67486080_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15 67.49 Mbp_chr15_67486080_R Primer3: not found at this Tm N.d. 0.13
mm4__chr12 120.40 Mbp_chr12_120402951_F Primer3: not found at this Tm N.d. 0.13
mm4__chr12 120.40 Mbp_chr12_120402951_R Primer3: not found at this Tm N.d. 0.13
mm4__chr1 35.93 Mbp_chr1_35931294_F TCGTCGGCAGCGTCCCTTCCTCTCGCCTAGTCCT 60.1 0.13
mm4__chr1 35.93 Mbp_chr1_35931294_R GTCTCGTGGGCTCGGGACGGGCACTCTCACTCG 59.8 0.13
mm3__chr7 156.54 Mbp_chr7_156540203_F TCGTCGGCAGCGTCTGTGGTGTCTGCATCATGTGA 59.9 0.13
mm3__chr7 156.54 Mbp_chr7_156540203_R GTCTCGTGGGCTCGGAGGAATGCCATTAGCCACGT 59.7 0.13
mm4__chr17 65.14 Mbp_chr17_65137443_F TCGTCGGCAGCGTCCGCCTAGTGAGAGTCAGGGG 61.3 0.13
mm4__chr17 65.14 Mbp_chr17_65137443_R GTCTCGTGGGCTCGGACCAGTGGGAGACAAGAGGA 59.8 0.13
mm4__chr17 65.10 Mbp_chr17_65100887_F TCGTCGGCAGCGTCCCTAGTGAGGGTCAGGGAGG 60.3 0.13
mm4__chr17 65.10 Mbp_chr17_65100887_R GTCTCGTGGGCTCGGGAGCACCAGTGGGAGACAAG 60.3 0.13
mm4__chr15 22.96 Mbp_chr15_22958135_F Primer3: not found at this Tm N.d. 0.12
mm4__chr15 22.96 Mbp_chr15_22958135_R Primer3: not found at this Tm N.d. 0.12
mm4__chr10 93.00 Mbp_chr10_92996561_F Primer3: not found at this Tm N.d. 0.12
mm4__chr10 93.00 Mbp_chr10_92996561_R Primer3: not found at this Tm N.d. 0.12
mm4__chr4 121.70 Mbp_chr4_121697077_F Primer3: not found at this Tm N.d. 0.12
mm4__chr4 121.70 Mbp_chr4_121697077_R Primer3: not found at this Tm N.d. 0.12
mm4__chr2 109.38 Mbp_chr2_109375037_F TCGTCGGCAGCGTCGCACTGCTTCAGCTGCTTTC 60.3 0.12
mm4__chr2 109.38 Mbp_chr2_109375037_R GTCTCGTGGGCTCGGTCATGGGTGAATGCCTGTGG 60.3 0.12
mm4__chr22 46.13 Mbp_chr22_46126060_F Primer3: not found at this Tm N.d. 0.12
mm4__chr22 46.13 Mbp_chr22_46126060_R Primer3: not found at this Tm N.d. 0.12
mm4__chr7 522.55 Kbp_chr7_522546_F TCGTCGGCAGCGTCCCAGCCGCTATTAGTCGGTT 59.8 0.11
mm4__chr7 522.55 Kbp_chr7_522546_R GTCTCGTGGGCTCGGGTCAGCGCCTCTGTCTCC 59.8 0.11
mm4__chrX 47.97 Mbp_chrX_47971957_F Primer3: not found at this Tm N.d. 0.11
mm4__chrX 47.97 Mbp_chrX_47971957_R Primer3: not found at this Tm N.d. 0.11
mm3__chr4 152.94 Mbp_chr4_152936428_F TCGTCGGCAGCGTCGGCTACGCCGACACAACG 61.4 0.11
mm3__chr4 152.94 Mbp_chr4_152936428_R GTCTCGTGGGCTCGGCTCCAGCTCCCCGTGTCC 61.7 0.11
mm4__chrX 16.85 Mbp_chrX_16853932_F Primer3: not found at this Tm N.d. 0.11
mm4__chrX 16.85 Mbp_chrX_16853932_R Primer3: not found at this Tm N.d. 0.11
mm4__chr7 7.58 Mbp_chr7_7580736_F Primer3: not found at this Tm N.d. 0.11
mm4__chr7 7.58 Mbp_chr7_7580736_R Primer3: not found at this Tm N.d. 0.11
mm4__chr4 128.63 Mbp_chr4_128633472_F Primer3: not found at this Tm N.d. 0.11
mm4__chr4 128.63 Mbp_chr4_128633472_R Primer3: not found at this Tm N.d. 0.11
mm4__chr2 121.11 Mbp_chr2_121113007_F Primer3: not found at this Tm N.d. 0.11
mm4__chr2 121.11 Mbp_chr2_121113007_R Primer3: not found at this Tm N.d. 0.11
mm4__chr2 112.24 Mbp_chr2_112236208_F Primer3: not found at this Tm N.d. 0.11
mm4__chr2 112.24 Mbp_chr2_112236208_R Primer3: not found at this Tm N.d. 0.11
mm4__chr19 3.88 Mbp_chr19_3876987_F Primer3: not found at this Tm N.d. 0.11
mm4__chr19 3.88 Mbp_chr19_3876987_R Primer3: not found at this Tm N.d. 0.11
mm4__chr19 18.05 Mbp_chr19_18051398_F Primer3: not found at this Tm N.d. 0.11
mm4__chr19 18.05 Mbp_chr19_18051398_R Primer3: not found at this Tm N.d. 0.11
mm4__chr11 10.50 Mbp_chr11_10499263_F Primer3: not found at this Tm N.d. 0.11
mm4__chr11 10.50 Mbp_chr11_10499263_R Primer3: not found at this Tm N.d. 0.11
mm4__chr10 133.62 Mbp_chr10_133618777_F Primer3: not found at this Tm N.d. 0.11
mm4__chr10 133.62 Mbp_chr10_133618777_R Primer3: not found at this Tm N.d. 0.11
mm4__chrX 153.08 Mbp_chrX_153080309_F TCGTCGGCAGCGTCGAGTCCTTCTTCAGAAAGGGCA 59.9 0.10
mm4__chrX 153.08 Mbp_chrX_153080309_R GTCTCGTGGGCTCGGGGAAATTAGTTGCGAGCCGG 59.6 0.10
mm4__chrX 153.07 Mbp_chrX_153069699_F TCGTCGGCAGCGTCGGAAATTAGTTGCGAGCCGG 59.6 0.10
mm4__chrX 153.07 Mbp_chrX_153069699_R GTCTCGTGGGCTCGGGAGTCCTTCTTCAGAAAGGGCA 59.9 0.10
mm4__chr10 76.64 Mbp_chr10_76643600_F TCGTCGGCAGCGTCTAGTTTGGCTCATCCCTGGC 59.7 0.10
mm4__chr10 76.64 Mbp_chr10_76643600_R GTCTCGTGGGCTCGGTGATCACCAGAGGCAGAGGA 59.9 0.10
mm4__chr5 157.27 Mbp_chr5_157266085_F Primer3: not found at this Tm N.d. 0.10
mm4__chr5 157.27 Mbp_chr5_157266085_R Primer3: not found at this Tm N.d. 0.10
mm4__chr10 131.88 Mbp_chr10_131880901_F TCGTCGGCAGCGTCGGCTCCTCTTCTCCCTCCTT 60.3 0.10
mm4__chr10 131.88 Mbp_chr10_131880901_R GTCTCGTGGGCTCGGTAACCAGGAAGCTCCCCAGT 60.1 0.10
mm4__chr8 125.43 Mbp_chr8_125430874_F TCGTCGGCAGCGTCGCTGGAAGAAGTCGCGGAG 60.8 0.10
mm4__chr8 125.43 Mbp_chr8_125430874_R GTCTCGTGGGCTCGGCTCATGGCAGAGCGCACC 61.2 0.10
mm4__chr2 218.66 Mbp_chr2_218659663_F Primer3: not found at this Tm N.d. 0.10
mm4__chr2 218.66 Mbp_chr2_218659663_R Primer3: not found at this Tm N.d. 0.10
mm4__chr8 105.94 Mbp_chr8_105935397_F Primer3: not found at this Tm N.d. 0.10
mm4__chr8 105.94 Mbp_chr8_105935397_R Primer3: not found at this Tm N.d. 0.10
mm4__chr19 38.40 Mbp_chr19_38395547_F Primer3: not found at this Tm N.d. 0.09
mm4__chr19 38.40 Mbp_chr19_38395547_R Primer3: not found at this Tm N.d. 0.09
mm4__chr7 150.37 Mbp_chr7_150372200_F TCGTCGGCAGCGTCATTCACAAGCGATCCGAGGG 60.1 0.09
mm4__chr7 150.37 Mbp_chr7_150372200_R GTCTCGTGGGCTCGGGGCCGGTTTCAGAGGTACC 60.0 0.09
mm4__chr5 150.52 Mbp_chr5_150519966_F TCGTCGGCAGCGTCAACCCAGAGTTATGGCTGCC 60.0 0.08
mm4__chr5 150.52 Mbp_chr5_150519966_R GTCTCGTGGGCTCGGCTGAGGATCGCCATACTGCC 60.3 0.08
mm4__chr1 55.21 Mbp_chr1_55214919_F Primer3: not found at this Tm N.d. 0.08
mm4__chr1 55.21 Mbp_chr1_55214919_R Primer3: not found at this Tm N.d. 0.08
mm4__chr4 151.69 Mbp_chr4_151690356_F TCGTCGGCAGCGTCTGGTGACAAGCCTGCTGAAT 59.8 0.08
mm4__chr4 151.69 Mbp_chr4_151690356_R GTCTCGTGGGCTCGGATCTGTAGTCAAGCTTGGGC 57.5 0.08
mm4__chr1 223.85 Mbp_chr1_223845852_F Primer3: not found at this Tm N.d. 0.08
mm4__chr1 223.85 Mbp_chr1_223845852_R Primer3: not found at this Tm N.d. 0.08
mm4__chr14 90.06 Mbp_chr14_90061222_F TCGTCGGCAGCGTCCCAGTGTTTGGCCTCCTCTC 60.3 0.08
mm4__chr14 90.06 Mbp_chr14_90061222_R GTCTCGTGGGCTCGGCGCCTTGACCATGCCAGA 60.0 0.08
mm4__chr3 183.83 Mbp_chr3_183825679_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 183.83 Mbp_chr3_183825679_R Primer3: not found at this Tm N.d. 0.08
mm4__chr22 18.97 Mbp_chr22_18970815_F TCGTCGGCAGCGTCCCGTCCAGGGCTTCCAAG 60.0 0.08
mm4__chr22 18.97 Mbp_chr22_18970815_R GTCTCGTGGGCTCGGGCAACTTTTCCTGCTGCCTC 60.0 0.08
mm4__chr12 109.71 Mbp_chr12_109714660_F TCGTCGGCAGCGTCCGAAGATGCGTCGCCTGAG 61.2 0.08
mm4__chr12 109.71 Mbp_chr12_109714660_R GTCTCGTGGGCTCGGGCTGTCGGTGAGGGCTATC 59.9 0.08
mm4__chr11 66.46 Mbp_chr11_66463238_F Primer3: not found at this Tm N.d. 0.08
mm4__chr11 66.46 Mbp_chr11_66463238_R Primer3: not found at this Tm N.d. 0.08
mm4__chr18 44.18 Mbp_chr18_44179790_F Primer3: not found at this Tm N.d. 0.08
mm4__chr18 44.18 Mbp_chr18_44179790_R Primer3: not found at this Tm N.d. 0.08
mm4__chr8 143.38 Mbp_chr8_143382099_F TCGTCGGCAGCGTCGAGCAACATTGGGAAGGGGA 59.9 0.07
mm4__chr8 143.38 Mbp_chr8_143382099_R GTCTCGTGGGCTCGGCAAGAGCTGCCAGATGGAGG 60.4 0.07
mm4__chr7 125.52 Mbp_chr7_125521946_F Primer3: not found at this Tm N.d. 0.07
mm4__chr7 125.52 Mbp_chr7_125521946_R Primer3: not found at this Tm N.d. 0.07
mm4__chr7 44.31 Mbp_chr7_44309999_F TCGTCGGCAGCGTCACGCGGGAAGAGCCTTTC 60.0 0.07
mm4__chr7 44.31 Mbp_chr7_44309999_R GTCTCGTGGGCTCGGATCCTAACTCCCAAAGCGCC 60.1 0.07
mm4__chr19 3.55 Mbp_chr19_3547197_F TCGTCGGCAGCGTCTTCTACAAGGCGGGAACTCG 59.7 0.07
mm4__chr19 3.55 Mbp_chr19_3547197_R GTCTCGTGGGCTCGGTGCATTGGGAGGAACGTGAG 60.3 0.07
mm4__chr19 18.00 Mbp_chr19_18001141_F TCGTCGGCAGCGTCGATGCAGAGGACCAGGAAGT 59.0 0.07
mm4__chr19 18.00 Mbp_chr19_18001141_R GTCTCGTGGGCTCGGGCAGATCCCCGGGGTTTC 60.1 0.07
mm4__chr4 1.80 Mbp_chr4_1799316_F TCGTCGGCAGCGTCAGCAGGAGCAGTTGGTCTTC 59.9 0.07
mm4__chr4 1.80 Mbp_chr4_1799316_R GTCTCGTGGGCTCGGGCCATCCTTGACCCAGACAG 60.3 0.07
mm4__chr1 40.22 Mbp_chr1_40224931_F Primer3: not found at this Tm N.d. 0.07
mm4__chr1 40.22 Mbp_chr1_40224931_R Primer3: not found at this Tm N.d. 0.07
mm4__chr19 1.00 Mbp_chr19_1001818_F TCGTCGGCAGCGTCCTAGGGCTGGTGGGAGAGG 60.7 0.07
mm4__chr19 1.00 Mbp_chr19_1001818_R GTCTCGTGGGCTCGGGCCAAGCCAGTCCCTAAGAC 60.3 0.07
mm3__chr1 31.73 Mbp_chr1_31726966_F TCGTCGGCAGCGTCCAGGGACAAAGATGGGGTCA 59.3 0.06
mm3__chr1 31.73 Mbp_chr1_31726966_R GTCTCGTGGGCTCGGGGGACCCCGACTTTGTTTCT 59.8 0.06
mm4__chr11 13.26 Mbp_chr11_13261032_F TCGTCGGCAGCGTCCAGTCAGCTTCCGCTCTTCC 60.7 0.06
mm4__chr11 13.26 Mbp_chr11_13261032_R GTCTCGTGGGCTCGGATTTAGGGGTGTGCACGAGG 60.0 0.06
mm4__chr16 12.39 Mbp_chr16_12386010_F Primer3: not found at this Tm N.d. 0.06
mm4__chr16 12.39 Mbp_chr16_12386010_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 157.69 Mbp_chr7_157685235_F TCGTCGGCAGCGTCCCTCCCACCCCTCATCCC 60.7 0.06
mm4__chr7 157.69 Mbp_chr7_157685235_R GTCTCGTGGGCTCGGAGTCATCCACGTGAGCGC 60.1 0.06
mm4__chr2 7.95 Mbp_chr2_7950860_F TCGTCGGCAGCGTCCAGCTGGGTAACTCAGAGGC 60.1 0.06
mm4__chr2 7.95 Mbp_chr2_7950860_R GTCTCGTGGGCTCGGTAAGTGGCCACGTTCGTTCT 59.6 0.06
mm4__chr22 29.07 Mbp_chr22_29073669_F TCGTCGGCAGCGTCGTACCTCCTGGGATCCCGT 60.0 0.06
mm4__chr22 29.07 Mbp_chr22_29073669_R GTCTCGTGGGCTCGGGATCCTAGGAGGTACCGGGG 60.2 0.06
mm4__chr16 2.93 Mbp_chr16_2932566_F TCGTCGGCAGCGTCTAACTGGGACATGGGCTTGG 59.6 0.06
mm4__chr16 2.93 Mbp_chr16_2932566_R GTCTCGTGGGCTCGGAAGTCTCCCTGTTCCCTGCA 60.7 0.06
mm4__chr1 940.43 Kbp_chr1_940434_F Primer3: not found at this Tm N.d. 0.06
mm4__chr1 940.43 Kbp_chr1_940434_R Primer3: not found at this Tm N.d. 0.06
mm4__chr14 85.03 Mbp_chr14_85034486_F Primer3: not found at this Tm N.d. 0.06
mm4__chr14 85.03 Mbp_chr14_85034486_R Primer3: not found at this Tm N.d. 0.06
mm4__chr10 12.79 Mbp_chr10_12788786_F TCGTCGGCAGCGTCATACGCTCTTAGCAGTCGGG 59.3 0.06
mm4__chr10 12.79 Mbp_chr10_12788786_R GTCTCGTGGGCTCGGGCGGCAGCAAAATGAGAGAC 60.1 0.06
mm4__chr8 1.25 Mbp_chr8_1254230_F TCGTCGGCAGCGTCCCTGAAATCAGTGCCTCCCA 59.6 0.05
mm4__chr8 1.25 Mbp_chr8_1254230_R GTCTCGTGGGCTCGGTGGACCCATCAGAGGTCACA 60.1 0.05
mm4__chr10 5.81 Mbp_chr10_5813155_F Primer3: not found at this Tm N.d. 0.05
mm4__chr10 5.81 Mbp_chr10_5813155_R Primer3: not found at this Tm N.d. 0.05
mm3__chr22 36.82 Mbp_chr22_36816787_F Primer3: not found at this Tm N.d. 0.05
mm3__chr22 36.82 Mbp_chr22_36816787_R Primer3: not found at this Tm N.d. 0.05
mm4__chr10 128.51 Mbp_chr10_128511241_F TCGTCGGCAGCGTCAGGAAGTCCAGTGCAAGTGT 59.1 0.05
mm4__chr10 128.51 Mbp_chr10_128511241_R GTCTCGTGGGCTCGGCATGGTCCTGTGGATGGGAT 59.1 0.05
mm3__chr12 131.93 Mbp_chr12_131929984_F Primer3: not found at this Tm N.d. 0.05
mm3__chr12 131.93 Mbp_chr12_131929984_R Primer3: not found at this Tm N.d. 0.05
mm4__chr11 126.42 Mbp_chr11_126416868_F TCGTCGGCAGCGTCGAATCTCGCAGGGCCTGTC 60.5 0.05
mm4__chr11 126.42 Mbp_chr11_126416868_R GTCTCGTGGGCTCGGCAAGCCTTGAGCCGCTTG 59.4 0.05
mm4__chr10 102.23 Mbp_chr10_102231893_F TCGTCGGCAGCGTCCCAAGCCAGCGCATATTCTC 59.4 0.05
mm4__chr10 102.23 Mbp_chr10_102231893_R GTCTCGTGGGCTCGGGGGTGCAGGACATAACAGCT 60.0 0.05
mm4__chr22 29.07 Mbp_chr22_29073807_F TCGTCGGCAGCGTCGTACCTCCTGGGATCCCGT 60.0 0.05
mm4__chr22 29.07 Mbp_chr22_29073807_R GTCTCGTGGGCTCGGTGCGTCGATGAAGGACTTGG 60.3 0.05
mm4__chr1 169.89 Mbp_chr1_169893647_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 169.89 Mbp_chr1_169893647_R Primer3: not found at this Tm N.d. 0.04
mm4__chr7 157.82 Mbp_chr7_157824199_F TCGTCGGCAGCGTCCAGAGCTCAGACCGTCAGTG 60.1 0.04
mm4__chr7 157.82 Mbp_chr7_157824199_R GTCTCGTGGGCTCGGTCAGGAGGGTGACTCTCTGG 59.9 0.04
mm3__chr11 46.38 Mbp_chr11_46384541_F TCGTCGGCAGCGTCAATGTGACAAGGGCAGTGCT 60.1 0.04
mm3__chr11 46.38 Mbp_chr11_46384541_R GTCTCGTGGGCTCGGTCTCCCCAAATCCCACTCCA 60.1 0.04
mm4__chr20 38.60 Mbp_chr20_38602052_F TCGTCGGCAGCGTCCCTAGCCTCACCGTGTCCTC 61.6 0.04
mm4__chr20 38.60 Mbp_chr20_38602052_R GTCTCGTGGGCTCGGTGGTGCATAGTAGGGCCAAC 59.7 0.04
mm4__chr15 29.27 Mbp_chr15_29269473_F Primer3: not found at this Tm N.d. 0.04
mm4__chr15 29.27 Mbp_chr15_29269473_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 100.57 Mbp_chr14_100568220_F TCGTCGGCAGCGTCCTCCCCGCACCGAGTTAC 59.8 0.04
mm4__chr14 100.57 Mbp_chr14_100568220_R GTCTCGTGGGCTCGGGAGAGTTAACGGGGACAGGC 60.1 0.04
mm4__chrX 54.22 Mbp_chrX_54219622_F Primer3: not found at this Tm N.d. 0.04
mm4__chrX 54.22 Mbp_chrX_54219622_R Primer3: not found at this Tm N.d. 0.04
mm4__chr7 40.04 Mbp_chr7_40042129_F Primer3: not found at this Tm N.d. 0.04
mm4__chr7 40.04 Mbp_chr7_40042129_R Primer3: not found at this Tm N.d. 0.04
mm4__chr6 67.52 Mbp_chr6_67524999_F Primer3: not found at this Tm N.d. 0.04
mm4__chr6 67.52 Mbp_chr6_67524999_R Primer3: not found at this Tm N.d. 0.04
mm4__chr6 25.48 Mbp_chr6_25480770_F Primer3: not found at this Tm N.d. 0.04
mm4__chr6 25.48 Mbp_chr6_25480770_R Primer3: not found at this Tm N.d. 0.04
mm4__chr3 171.20 Mbp_chr3_171196804_F Primer3: not found at this Tm N.d. 0.04
mm4__chr3 171.20 Mbp_chr3_171196804_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 148.59 Mbp_chr2_148593865_F Primer3: not found at this Tm N.d. 0.04
mm4__chr2 148.59 Mbp_chr2_148593865_R Primer3: not found at this Tm N.d. 0.04
mm4__chr19 13.90 Mbp_chr19_13902580_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 13.90 Mbp_chr19_13902580_R Primer3: not found at this Tm N.d. 0.04
mm4__chr17 62.84 Mbp_chr17_62836443_F Primer3: not found at this Tm N.d. 0.04
mm4__chr17 62.84 Mbp_chr17_62836443_R Primer3: not found at this Tm N.d. 0.04
mm4__chr17 46.27 Mbp_chr17_46269476_F Primer3: not found at this Tm N.d. 0.04
mm4__chr17 46.27 Mbp_chr17_46269476_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 35.54 Mbp_chr14_35538015_F Primer3: not found at this Tm N.d. 0.04
mm4__chr14 35.54 Mbp_chr14_35538015_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 240.70 Mbp_chr1_240696959_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 240.70 Mbp_chr1_240696959_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 220.08 Mbp_chr1_220083364_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 220.08 Mbp_chr1_220083364_R Primer3: not found at this Tm N.d. 0.04
mm4__chr5 135.03 Mbp_chr5_135027612_F TCGTCGGCAGCGTCCAGGCTGAGCAGGTCAAGAA 59.9 0.04
mm4__chr5 135.03 Mbp_chr5_135027612_R GTCTCGTGGGCTCGGGAGGAGGGAGGCGGATAGAA 60.1 0.04
mm4__chr9 135.53 Mbp_chr9_135526339_F TCGTCGGCAGCGTCCTGTCCTGGCCTCACTCAC 59.7 0.04
mm4__chr9 135.53 Mbp_chr9_135526339_R GTCTCGTGGGCTCGGAATGACGGAGGTGCTGGATG 60.1 0.04
mm4__chr3 36.76 Mbp_chr3_36764215_F TCGTCGGCAGCGTCGAGCGCACGGTACTCACC 60.5 0.03
mm4__chr3 36.76 Mbp_chr3_36764215_R GTCTCGTGGGCTCGGCACCTCTGCTCCAGGCTCT 60.9 0.03
mm4__chr6 137.22 Mbp_chr6_137219133_F TCGTCGGCAGCGTCAACCACGGAGCCCCAGTC 61.6 0.03
mm4__chr6 137.22 Mbp_chr6_137219133_R GTCTCGTGGGCTCGGGAGAGGGAGGGAGGGATCC 59.8 0.03
mm4__chr4 54.23 Mbp_chr4_54226198_F TCGTCGGCAGCGTCAAGCCAACTCAGTGGGAAGC 60.5 0.03
mm4__chr4 54.23 Mbp_chr4_54226198_R GTCTCGTGGGCTCGGAAAGCAAAATCTCGCCGTGC 60.3 0.03
mm4__chr12 32.90 Mbp_chr12_32896873_F Primer3: not found at this Tm N.d. 0.03
mm4__chr12 32.90 Mbp_chr12_32896873_R Primer3: not found at this Tm N.d. 0.03
mm4__chr19 47.29 Mbp_chr19_47291322_F Primer3: not found at this Tm N.d. 0.03
mm4__chr19 47.29 Mbp_chr19_47291322_R Primer3: not found at this Tm N.d. 0.03
mm4__chr10 101.09 Mbp_chr10_101087582_F TCGTCGGCAGCGTCCTCTGACTCTGTTTGGGGCA 59.6 0.03
mm4__chr10 101.09 Mbp_chr10_101087582_R GTCTCGTGGGCTCGGATGGGGACGCCATTACTGTG 60.1 0.03
mm3__chr7 76.28 Mbp_chr7_76281441_F Primer3: not found at this Tm N.d. 0.03
mm3__chr7 76.28 Mbp_chr7_76281441_R Primer3: not found at this Tm N.d. 0.03
mm4__chr8 8.70 Mbp_chr8_8701891_F TCGTCGGCAGCGTCCTCTTTCCCTCCCCGCCC 61.7 0.02
mm4__chr8 8.70 Mbp_chr8_8701891_R GTCTCGTGGGCTCGGCTGGCCTGGACTGCTCAC 60.0 0.02
mm4__chr14 99.79 Mbp_chr14_99793258_F Primer3: not found at this Tm N.d. 0.02
mm4__chr14 99.79 Mbp_chr14_99793258_R Primer3: not found at this Tm N.d. 0.02
mm3__chr7 2.56 Mbp_chr7_2555363_F Primer3: not found at this Tm N.d. 0.02
mm3__chr7 2.56 Mbp_chr7_2555363_R Primer3: not found at this Tm N.d. 0.02
mm4__chr6 41.10 Mbp_chr6_41101128_F TCGTCGGCAGCGTCACTGGTCTTTCGGAAGCCTC 59.6 0.02
mm4__chr6 41.10 Mbp_chr6_41101128_R GTCTCGTGGGCTCGGGTCACATGGACGCCTAGGAG 59.8 0.02
mm4__chr9 16.19 Mbp_chr9_16192353_F TCGTCGGCAGCGTCCTTGGGGCTTGGTCCAGATT 59.9 0.02
mm4__chr9 16.19 Mbp_chr9_16192353_R GTCTCGTGGGCTCGGTCCTTGGCCTTCCTGAACAC 59.8 0.02
mm4__chr17 81.87 Mbp_chr17_81871580_F Primer3: not found at this Tm N.d. 0.02
mm4__chr17 81.87 Mbp_chr17_81871580_R Primer3: not found at this Tm N.d. 0.02
mm4__chr12 130.93 Mbp_chr12_130933852_F TCGTCGGCAGCGTCGGATGACTGCAACCTGCTCT 60.0 0.02
mm4__chr12 130.93 Mbp_chr12_130933852_R GTCTCGTGGGCTCGGGCAGAACCTGGAGGAAGGAG 59.7 0.02
mm4__chr7 66.95 Mbp_chr7_66950170_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 66.95 Mbp_chr7_66950170_R Primer3: not found at this Tm N.d. 0.01
mm3__chr10 63.63 Mbp_chr10_63630244_F TCGTCGGCAGCGTCGGAGCCACATCTGATGCACT 60.1 0.01
mm3__chr10 63.63 Mbp_chr10_63630244_R GTCTCGTGGGCTCGGCTCGCATCTGGACTCCCTCT 60.7 0.01
mm4__chr2 20.64 Mbp_chr2_20638317_F TCGTCGGCAGCGTCCACGCAGAGAGCTACTTCCC 60.1 0.01
mm4__chr2 20.64 Mbp_chr2_20638317_R GTCTCGTGGGCTCGGTTTCTGGAGATGACGTCCCC 59.0 0.01
mm4__chr19 55.15 Mbp_chr19_55147206_F Primer3: not found at this Tm N.d. 0.01
mm4__chr19 55.15 Mbp_chr19_55147206_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 229.43 Mbp_chr1_229431967_F TCGTCGGCAGCGTCAGGTGGTCTCGTGAATGCC 59.7 0.01
mm4__chr1 229.43 Mbp_chr1_229431967_R GTCTCGTGGGCTCGGCAGCCCTCCTTCATCGGTG 60.1 0.01
mm4__chr1 38.05 Mbp_chr1_38046028_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 38.05 Mbp_chr1_38046028_R Primer3: not found at this Tm N.d. 0.01
mm4__chr10 12.41 Mbp_chr10_12411172_F TCGTCGGCAGCGTCCTGGGTGCCCACTAACCATC 60.3 0.01
mm4__chr10 12.41 Mbp_chr10_12411172_R GTCTCGTGGGCTCGGCCAAGCCTTATGCCCATGGA 60.1 0.01
mm4__chr12 121.84 Mbp_chr12_121839560_F TCGTCGGCAGCGTCGCTTTAATCGGGAGGGCTGG 60.8 0.01
mm4__chr12 121.84 Mbp_chr12_121839560_R GTCTCGTGGGCTCGGTCTGGAACTCGCCCAACTTC 59.9 0.01
mm4__chr11 86.50 Mbp_chr11_86498072_F TCGTCGGCAGCGTCTGTACAGGGCCAGCTTGC 59.9 0.01
mm4__chr11 86.50 Mbp_chr11_86498072_R GTCTCGTGGGCTCGGTGTCCCTGATTGATGGTGCC 60.0 0.01
mm4__chr7 151.88 Mbp_chr7_151878077_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 151.88 Mbp_chr7_151878077_R Primer3: not found at this Tm N.d. 0.01
mm4__chr6 154.51 Mbp_chr6_154510188_F TCGTCGGCAGCGTCGCAGATAAAGTGCTGCTGCC 59.9 0.01
mm4__chr6 154.51 Mbp_chr6_154510188_R GTCTCGTGGGCTCGGGCCATGCAGTCTTGACGGAG 61.3 0.01
mm4__chr4 17.81 Mbp_chr4_17811348_F TCGTCGGCAGCGTCTCACCTTCGCGACTCACTTC 60.0 0.00
mm4__chr4 17.81 Mbp_chr4_17811348_R GTCTCGTGGGCTCGGGGCTTCGGAGGCTTTCTGG 60.7 0.00
mm4__chr6 151.45 Mbp_chr6_151452654_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 151.45 Mbp_chr6_151452654_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 124.26 Mbp_chr9_124258063_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 124.26 Mbp_chr9_124258063_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 81.34 Mbp_chr17_81341581_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 81.34 Mbp_chr17_81341581_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 74.83 Mbp_chr2_74834842_F TCGTCGGCAGCGTCCCACGTCCGCTAAGCACAG 61.0 0.00
mm4__chr2 74.83 Mbp_chr2_74834842_R GTCTCGTGGGCTCGGGGAGCTCAGGGAGGGAGG 60.4 0.00
mm4__chr9 96.03 Mbp_chr9_96027670_F TCGTCGGCAGCGTCACTCCACATGCACCTTCTC 57.0 0.00
mm4__chr9 96.03 Mbp_chr9_96027670_R GTCTCGTGGGCTCGGGGAGGGAAGCTAGGGAAACG 59.8 0.00
mm4__chr9 105.24 Mbp_chr9_105244658_F TCGTCGGCAGCGTCCAGGAGCAGAGCAGGAGAC 59.4 0.00
mm4__chr9 105.24 Mbp_chr9_105244658_R GTCTCGTGGGCTCGGCTGGTACTCAAGAGGCGGC 60.1 0.00
mm4__chr8 38.90 Mbp_chr8_38901697_F TCGTCGGCAGCGTCCAGCCCCTCCTCCAAGTG 59.3 0.00
mm4__chr8 38.90 Mbp_chr8_38901697_R GTCTCGTGGGCTCGGGAGAAGTTTCGCTGCTGCAA 59.4 0.00
mm4__chr5 176.81 Mbp_chr5_176810149_F TCGTCGGCAGCGTCGAGGACTGCAGCGGAACC 60.4 0.00
mm4__chr5 176.81 Mbp_chr5_176810149_R GTCTCGTGGGCTCGGCAGTCTGCGCACAGGTGG 61.0 0.00
mm4__chr4 147.48 Mbp_chr4_147481243_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 147.48 Mbp_chr4_147481243_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 45.64 Mbp_chr21_45643738_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 45.64 Mbp_chr21_45643738_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 21.71 Mbp_chr20_21709304_F TCGTCGGCAGCGTCCAACCAGCACGGCGTGTA 60.6 0.00
mm4__chr20 21.71 Mbp_chr20_21709304_R GTCTCGTGGGCTCGGCATTGCTGCCGCGAGTTC 59.9 0.00
mm4__chr2 27.07 Mbp_chr2_27071913_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 27.07 Mbp_chr2_27071913_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 120.35 Mbp_chr2_120345217_F TCGTCGGCAGCGTCGACGCTGCGAGACTTTTCG 59.5 0.00
mm4__chr2 120.35 Mbp_chr2_120345217_R GTCTCGTGGGCTCGGGCTCCTGTCTCTTCTGCCTG 60.1 0.00
mm4__chr18 79.40 Mbp_chr18_79400341_F TCGTCGGCAGCGTCTCACGTTACGCGGAGGAC 59.4 0.00
mm4__chr18 79.40 Mbp_chr18_79400341_R GTCTCGTGGGCTCGGTCGAAGTCGAACTCCTGGTC 59.1 0.00
mm4__chr17 74.35 Mbp_chr17_74350832_F TCGTCGGCAGCGTCCGACAGCGAGAAGGACTCAG 60.1 0.00
mm4__chr17 74.35 Mbp_chr17_74350832_R GTCTCGTGGGCTCGGAAACCCTGGACACCTTCTGC 60.1 0.00
mm4__chr16_KI270728v1_random 1.62 Mbp_chr16_KI270728v1_random_1616652_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16_KI270728v1_random 1.62 Mbp_chr16_KI270728v1_random_1616652_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16_KI270728v1_random 1.35 Mbp_chr16_KI270728v1_random_1345714_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16_KI270728v1_random 1.35 Mbp_chr16_KI270728v1_random_1345714_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 100.24 Mbp_chr14_100239204_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 100.24 Mbp_chr14_100239204_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 131.31 Mbp_chr10_131311518_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 131.31 Mbp_chr10_131311518_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 230.87 Mbp_chr1_230868482_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 230.87 Mbp_chr1_230868482_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 15.85 Mbp_chr1_15847962_F TCGTCGGCAGCGTCGGAGAGGGATGGTCTCTGCA 60.6 0.00
mm4__chr1 15.85 Mbp_chr1_15847962_R GTCTCGTGGGCTCGGCGGCTTCGTACGAGACCG 60.2 0.00
mm3__chr9 89.43 Mbp_chr9_89429226_F TCGTCGGCAGCGTCCTGGCTCTCCTAACCTCCCT 60.0 0.00
mm3__chr9 89.43 Mbp_chr9_89429226_R GTCTCGTGGGCTCGGGGCTTCTTGGCATCCAGAGT 60.0 0.00
mm3__chr21 6.12 Mbp_chr21_6117756_F Primer3: not found at this Tm N.d. 0.00
mm3__chr21 6.12 Mbp_chr21_6117756_R Primer3: not found at this Tm N.d. 0.00
mm3__chr21 43.42 Mbp_chr21_43420481_F TCGTCGGCAGCGTCGCGGATCAGGCTCTCACAG 60.2 0.00
mm3__chr21 43.42 Mbp_chr21_43420481_R GTCTCGTGGGCTCGGTGTCAAACCACGTGGGGC 60.5 0.00
mm3__chr19 1.24 Mbp_chr19_1241915_F Primer3: not found at this Tm N.d. 0.00
mm3__chr19 1.24 Mbp_chr19_1241915_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529689 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr4 144.65 Mbp_chr4_144645852 GGGCAGTCCCGGAATCTCCGGACTGCGTCCCTGCCCCAAGCCCGCCGGGAACCCGGGCCGTGGACGCCCCCACCCGGGGG
CCGCGGCGAGGCGGGCGCGCGGGGTTCAGCACCCTCGAGGCTGGTTCCGAAG
mm4__chr1 2.57 Mbp_chr1_2569571 GTTTCCCTCCTGCCACGAAAGCTCGAGACTGAAACTCAGGATGAACTGTTTTCAGCCCGGGTCACCCCAGCCCTGGGGTG
GAGGCCCATTGAGGATG
mm3__chr15 90.72 Mbp_chr15_90717639 TCCTGGGAGGACAGCAGATACATAAATACGTTCCCAAATGGGGAAACAGAGGCCCGGGGCGGGGGCGAACTCTGCCAAGG
TCTCCCGGGGCGTCGGAGGCCGAGCATGGGCTAGGACCTGGCATGTTGT
mm4__chr14 104.16 Mbp_chr14_104157867 CCCTGATGGCTTGTCGAAGGCCTGGGGCCGTGGTGGAGTCTGCACGTCAGCCCTGGTCACCCCCACCCCGGGCTCGGTGG
GGGGCTCCACAGGCCCCTCAGCTGCAGCCTCCTTCTTCAT
mm4__chr19 18.65 Mbp_chr19_18650182 TTCCACAGGGGTCAAAGACGGACCCGGGCGGGAGCGGTCTGGGCTGGATATCCGGAGAGTTCTGCTCTCCACGGGATG
mm4__chr17 36.88 Mbp_chr17_36884812 ATTGAGCACAGACCAGGCTCCCCCCCAGAGACGGGGCAGCCCCCGCCCAGGAGGGGAGAAGGCAAGGGGGCGGGAGCGGG
AGGGGTGGCCCCGCTCCACGCTCAGCTGCTTCTGGGTGCAGATGGAGACAG
mm3__chr17 28.81 Mbp_chr17_28812288 GCCCTGGAAAGGTGAGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGGGCAGCTGAGCCC
AGCTCACCCCCGCCCCGGGAGGATGGAAGAGAGGGGGCTTCTAGCGGTCTAGGCAG
mm4__chr16 1.63 Mbp_chr16_1627581 TTGAGTACGGTGCATGCTGTGGCCAGGGTCGCTCCCGCCCTGGGCTGCAGGTTAGGTTCTGCCCACCCCAGGTGGACCGG
ACTGCCGTGGCAGTGTGGCTTGATTCT
mm2__chr17 76.24 Mbp_chr17_76239976 CCTCGGTGACCCTGCTAGACCCGGCAGGACGCCCCTTGGGCTAATTCGCCCGGGGCGGGGGAGACCTCGGCCTTCTCGAA
GACCTCCCGCGCTCGAAGACCGTTTCGGAGCGTCCGCAACCACTGAGTCCCCGAAG
mm4__chr19 54.14 Mbp_chr19_54141270 CAGGTAGGGCGTGATAGCTGACCTGGGCGGGGGCTGCTGCGGCTTTCTTTAGGTTGGGCCTTTTACTGAGGA
mm4__chr9 99.22 Mbp_chr9_99221638 GAAAACCATGCGCACGTAGGCGCAGACGGCGGCGCCGCGGCCGCCCCAGCCCAGGCCTCGCGGCAGCCAGTCCCCGGCAC
AGCGCACCGGTAGCTCGCGGCTCTCGGCGAAACAGT
mm4__chr17 81.39 Mbp_chr17_81385048 CAACCTCAGCCAAGGGTCTCCACATACTTCTCTCTTCTGTAATTGGGTCTTCCCGCGGAGGCTGGGGCCTGGGCGGATGG
ATGCCCAGGGTGGGGGCTACCTCCTCTGGACTGCCGAGACCTCCCCAGCAATGAG
mm4__chr1 231.04 Mbp_chr1_231040390 CTCTCCTCGAGGCCCCTGCGCGGGCCCCGGGTCCGGGTGGCGTCCGCGTCCAGCAGCCGGGCTCGCCTCCACCCGGGTCT
CCTCCTCCCTCGCGCCCCTCCAGCGCCCTAATTCATCTCCAACTCCGCGTG
mm4__chr19 58.56 Mbp_chr19_58563230 ACCTTGCCACATACATCGCAACGGCCGCCCCTAACCACCCCGCCCCCAGTGCTGGGGCGGCCCCGGCTCCGGCCCCTGGG
GGAGCGCCAGCGGGTGGTGATCAGAG
mm4__chr11 550.01 Kbp_chr11_550012 CGCCTCTTTGCCAGCACTTAAGGTGAGTCTGGGCACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGGCTCAGAG
CCCCGCGCTGCCCAGGAACTCTACGCCTCCTACAACAACATCTCGGACCTGAGC
mm4__chr2 38.60 Mbp_chr2_38602463 TGTTACCGGCTGAGAGAAGCTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCGTCGCTTCCCGGCGGTTCTCGC
CTTCCTCGGCCGAGTAGTCGATCTCCCCCTCCTCGGTCTTGAGACGCTTG
mm4__chr10 101.84 Mbp_chr10_101840838 TGACCTCCTAACTCTGGGCAAATCTTGTCCACTTGACGGCGACTATCCCCACCGCAAAGCAGCTCCGTGCGCATGGCTCC
TCGCAGCCACACGGGGGCGCCCCCGCCCCGGCAACAATACTCAGGGCCGGA
mm4__chr7 987.17 Kbp_chr7_987173 CACCCTGGACATGGTCATGGCCGGGACGGAGACGACCTCGGCCACGCTGCAGTGGGCCGCACTTCTGAT
mm4__chr15 55.99 Mbp_chr15_55993577 TTCCGAACGCTTCCAGCAAACCGGACGCGCTCGCCCCCGCCCAGGGCAGGCAACTGTNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCTCCCTCAGCGACAGCAG
mm4__chr16 75.29 Mbp_chr16_75294979 TCCTCACCATAAAGCGTGCTGCGATTTGCCTGGGCGGAGGAGATCTCTGCTGCTAATTAATCTATGGAGAGAGCAAAAAT
TGCCATGGAAAATGGGAGACAAAATTGAATCTCTGC
mm4__chr19 9.91 Mbp_chr19_9913150 CCCGGACTCAGCTCAATTCTGCAGCCCTTGTCGCCTCCGCCCTGGGGCCGGAGCGAGCCTGGTACGCTATTCG
mm3__chr11 112.96 Mbp_chr11_112963576 CAAGCCTAGTCCGCCTGGCCCTGGGTCTCCGCTGCCACCTGGGCGGGGGCCACCCCGGTTATGCCCCTTTCCGCGGTGCC
CGTGGGTGCCGCGACGCGACCTGTCCACATGGGGC
mm4__chr11 69.70 Mbp_chr11_69702376 CCTCTGCATCCTTTCTGGGGTGGGGGCCCCGGGCAGGCGCGGCCACCCCCGCCCTGGCCCCTGCTCCGCCAGGGAGTCCG
GGGCTGCGTGTGAGGCCTGCGCGGGCCGAGACCTCACACAGCCTCCT
mm4__chr11 124.67 Mbp_chr11_124673768 CGCCTAGGACTGGGAAAGTGGGTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCCACCCTTTTGCAGTCC
TCGCAGCCTCCCGCGACCTCAGGACTGGGCTGTACT
mm4__chr14 95.39 Mbp_chr14_95389786 GCACGGGTCACAGAGTCTTTTCTTTCTGCTGCCGGGTTCGCCCCCGCACTGGAACTCCAGGGAATTGGGTCTCTGGAAGC
CTGGGCAATGAAT
mm4__chr11 45.41 Mbp_chr11_45407356 CATTCTTGTTGGCCGTCTGCCCCTGCATAAAGCAGGGGTCACCCCTGCCCTGGAGGGACAGCTCTCCTACGTGGGTGGGC
AGCATCCTCAGACCCTTGG
mm4__chr11 19.89 Mbp_chr11_19887891 TGTAATCATCTCGCCTGGCAAAAAGCATCTCTGATTATGACCGCCCCCCGCCAAACCCCAGCATGTGCACACAAGCTGCC
ATGCTTCCTGGGAGGGGGCATCCTCTGCTCCCAGCCTCTGAAGTGGTTT
mm4__chr17 4.59 Mbp_chr17_4593372 TGCCCAGAGATGCTGTGAAATAGCGTCATCCCCTGGTGGGGGCGACCGTGGCTTTTATTACAGTTGGAAGGTGGCCTCTT
mm4__chr5 139.27 Mbp_chr5_139274069 CGAAGCCACGCCCAGTAGCCGCCCCGGGGCGGGGTTCCCCTCGGCTCCCGGCTGCCCTTTCCCCTCCGGCCTCTGCCGGT
GCTGCTGCGCCCTGCGGAGCTCCGAACACGTGCGCGTGAGTAAGGACCCAGAG
mm4__chr2 70.30 Mbp_chr2_70301673 TAAGAGGGGAAGGTGGTGGGGCGAGCGCCTGCCACCAGGGGCTTCCCACCTGCCGCCGAGGACGCTCCTGCCCCGGGCTG
GAAGGGGCGGAAAACAGCCTCGCGCACGGCAAGGGCACACGGATTTCATCCGATCCGCCA
mm4__chr19 402.17 Kbp_chr19_402174 TAATCGAATCCGGCACTGGGGCGGTGGGTGCCGCAGAGAGAGGCCGAGGGAGCCCCCGCCTGGGTGGGTCTGAGGCCGCC
TGTAATTATGGCCCTGATGTGATTATATTAATTATCGTCGCTATTTTGTGCCGGCTTCCG
mm4__chr3 49.43 Mbp_chr3_49429053 CGGCAGAGGCTTTCCCATAAAAGATTAAATGCCTGGGAAACGACCGAGATTCCAGGTCGGCCCCGCCCGGGAGCCTCGGT
CGCGCCCACCAGGCTTGCTCACCTCGAAGGGAG
mm4__chr17 50.20 Mbp_chr17_50200698 GCAACGCTAAGCAATGGGGCCGGGGCGGGGCCGACCTCACCGGCCCGACTACCTCTTTAAGAGGCTTGGGCTGCCCCATC
CCTGGCTTTACTT
mm4__chr11 126.41 Mbp_chr11_126406180 TGCCTGCTGAGAAACATGGTCAGCAAGTCCCGTGAGTGTCATCCGAGGGCTCCCCCACCCTGGAGGACAGGCCTCAGAAG
CCGTCTTCAGCAGGATCCTG
mm4__chr4 78.94 Mbp_chr4_78939293 TTCTGATGCATCGTTCCCGGCCGCCGCCGCTCCCCGGCTCGGGAGCTCCCCCAGGTCCCGCCTCCCCGGGGAGGGGGCTT
CGCCGCTGGCGCCCCCGCCCCGGAGCCCGCGGACGCTGCGCGAGGTCCTACCG
mm4__chr1 35.93 Mbp_chr1_35931294 CCTTCCTCTCGCCTAGTCCTGTGCCGTTTTCCGTCCGCGACTCTTCCGGCCCAGAGCTTTCGGAGTGCGGTTGCTCAGGG
GAAGCCGTCGCCGCCCCCGCCTCGGGGCCGAGTGAGAGTGCCCGTC
mm3__chr7 156.54 Mbp_chr7_156540203 TGTGGTGTCTGCATCATGTGACGCCGAGGTCGCCCCTGCCAGGGCAACGCACAGGCGGCAGGGCCCTTAAAAGTGCAGTC
AATAGGTCTGAAAATGATAGACGTGGCTAATGGCATTCCT
mm4__chr17 65.14 Mbp_chr17_65137443 CGCCTAGTGAGAGTCAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGCTGGGGCGA
GCCAGGCTGCCTTTCGAACTTGGGGGGCTTCTCCTCTTGTCTCCCACTGGT
mm4__chr17 65.10 Mbp_chr17_65100887 CCTAGTGAGGGTCAGGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGACCACCCAGGGCTGGGGCGAG
CCAGGCTGCCTTTCGAACTTGGGGGCTTCTCCTCTTGTCTCCCACTGGTGCTC
mm4__chr2 109.38 Mbp_chr2_109375037 GCACTGCTTCAGCTGCTTTCAGAGGAGGTGGACCCCGCCCTGGACATACGTACATTTCCCTCTGCTGTTCTATAGAAATA
ATCGTCTCTCCACAGCCCCTGGCACCCACCTGCCACAGGCATTCACCCATGA
mm4__chr7 522.55 Kbp_chr7_522546 CCAGCCGCTATTAGTCGGTTCACACAGTTCACTGCAAACATTTGATAATGAGGCTAAATATACTCCCGCGTCGGAGGAGG
CGTGGGCGTCCCCGCCCAGGCCCGGGAGACAGAGGCGCTGAC
mm3__chr4 152.94 Mbp_chr4_152936428 GGCTACGCCGACACAACGGCGGGAGGCTGCAGCGGTCTGCGCGCCGGGAGCGGGGGCGCGCTGGCCGCGGGTGAGTGCGG
GTCGGGCGGCCGAGGCGGGGGCGACCGCGGCGCGGGACACGGGGAGCTGGAG
mm4__chrX 153.08 Mbp_chrX_153080309 GAGTCCTTCTTCAGAAAGGGCACCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGAACATCTG
TGGGTCTGGTGTCTCCAACTGGCCCAGCCAGCCCACCGGCCGGCTCGCAACTAATTTCC
mm4__chrX 153.07 Mbp_chrX_153069699 GGAAATTAGTTGCGAGCCGGCCGGTGGGCTGGCTGGGCCAGTTGGAGACACCAGACCCACAGATGTTCCCANNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGTGCCCTTTCTGAAGAAGGACTC
mm4__chr10 76.64 Mbp_chr10_76643600 TAGTTTGGCTCATCCCTGGCACCTGCCCAGGTCGGCCCTGCCCCGGCGCCTCAGCACAGCCCTTCCTCTGCCTCTGGTGA
TCA
mm4__chr10 131.88 Mbp_chr10_131880901 GGCTCCTCTTCTCCCTCCTTGAGCCCTGCTCTGGTGGAGCAGAGGCCGGCCCTGCCCTGGGCAACTTTGCAGCCACCTTC
TAAAGCACTGGGGAGCTTCCTGGTTA
mm4__chr8 125.43 Mbp_chr8_125430874 GCTGGAAGAAGTCGCGGAGCCGGCACCAAACCCGCAGCGTCTTCCCGCGCGGATCCCGGGACTTAAAAAGCCGGGGCCAC
CCCGGCCCAGGACGGGATGCGGGTCGGTCCGGTGCGCTCTGCCATGAG
mm4__chr7 150.37 Mbp_chr7_150372200 ATTCACAAGCGATCCGAGGGGTCGGCCCAGGCCGCCCCCGGCCCGGGGAGCCCCCAGCTGCCAGCCGGCCCCCAGGAGTC
CGCGGCCGAGCCCACCCCGGCGGTACCTCTGAAACCGGCC
mm4__chr5 150.52 Mbp_chr5_150519966 AACCCAGAGTTATGGCTGCCTGGGTGGACAGAAGGTCCAGGGCGGGGGAGGCCTCCCCTCTGCATCCTGTGGTGGCAGTA
TGGCGATCCTCAG
mm4__chr4 151.69 Mbp_chr4_151690356 TGGTGACAAGCCTGCTGAATAATCCTGCAATCATCTCTTTAATCTCTTTTCAAGCCAGTGCTATTATTCTGCTCATGCTA
AACCAGGGCAGAGGCCACCTCGGCAGCCCAAGCTTGACTACAGAT
mm4__chr14 90.06 Mbp_chr14_90061222 CCAGTGTTTGGCCTCCTCTCCGGAAGCGGGGCTGGCTCGCGGTGCGCGCTTGGTCCGGGCGGAGCCTACTAGTAGGCGCA
GAGGACGCCCCCGCGCAGGATCCAGAGACCAAGGGCGCTCTGGCATGGTCAAGGCG
mm4__chr22 18.97 Mbp_chr22_18970815 CCGTCCAGGGCTTCCAAGGCTGGGGAAGCGTAGCCGGGCGGCCGGGAGGGCCACCGCAGTGCCGCGCCAGGCTGGGGCCA
AGGGCGCCCCGGCCCCGGGAGGGCGGAGCCGGAGGCAGCAGGAAAAGTTGC
mm4__chr12 109.71 Mbp_chr12_109714660 CGAAGATGCGTCGCCTGAGCGCCCGCGCGGCCCCCGTCCGGGGCGGGCGTGACCCCTGCTGAACGGAGACCTCCCCTCCC
CCCCGACTTCGGACGGCGCAGGCCGGCGCCAGGATAGCCCTCACCGACAGC
mm4__chr8 143.38 Mbp_chr8_143382099 GAGCAACATTGGGAAGGGGAGGTGGGGCTGCAGGTAACCCTCCCTGGGCCGCCTCCTGGGCAGGGGCCACCTGTGCTGTG
GCCTCCATCTGGCAGCTCTTG
mm4__chr7 44.31 Mbp_chr7_44309999 ACGCGGGAAGAGCCTTTCCGCCGGCTGAGCCGTGGGCGCCACCGCCAGGGACCCCAGGCTCACCAAGCCACGCGCGGAAC
CGCACGGTGGCCGAGACCGGGCGGGGGCGGGGGCGCTTTGGGAGTTAGGAT
mm4__chr19 3.55 Mbp_chr19_3547197 TTCTACAAGGCGGGAACTCGGAGGCCTGAGAGCATCCGAGGATGGAACCCGGAGCGGGTGGGATCTCGGCTGCAGGGCAC
CCCATCCTCCGCCCCAGCTGTCCCCGGTCCCCGCCCACTCACGTTCCTCCCAATGCA
mm4__chr19 18.00 Mbp_chr19_18001141 GATGCAGAGGACCAGGAAGTTCGCCGACGACGCGCCCGCCCTGGGCCACCGGCGCCGGCCCAAGTTCGCCGGGGGGGCCC
GGAGGAAGCTTGGGGGACGCGACGGGGGAACGCGGAAACCCCGGGGATCTGC
mm4__chr4 1.80 Mbp_chr4_1799316 AGCAGGAGCAGTTGGTCTTCGGCAGCGGGGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGGCCC
ACTGTCTGGGTCAAGGATGGC
mm4__chr19 1.00 Mbp_chr19_1001818 CTAGGGCTGGTGGGAGAGGCGGGGCCCAAACCCAGGCCTGGGGCGCCCTCTGCTGCCGTCTCCAGGAGCTGCACCGCCGC
AAGCCGGGGCGTGGGACCGACCTAGCGTCTTAGGGACTGGCTTGGC
mm3__chr1 31.73 Mbp_chr1_31726966 CAGGGACAAAGATGGGGTCAGAGCCTAGGCCGCCCCCTCCCAGGCAGGGCTGGACTGACCCCATCACATCATCTGATGAG
ATCAAGTGAGTTCAACCCAGGAGACAGAAACAAAGTCGGGGTCCC
mm4__chr11 13.26 Mbp_chr11_13261032 CAGTCAGCTTCCGCTCTTCCTCCATGGCAGGGCCGACCTCGGCACATCCACACTGCGGTTTTCTCTTGGAAGGAAGGATC
AGGCTAGCGGGGCAGTGTCCTCATGAGGCCTCGTGCACACCCCTAAAT
mm4__chr7 157.69 Mbp_chr7_157685235 CCTCCCACCCCTCATCCCGGCCGGGCCGATGGCCCCCGCGCGCCTCCATCCGCCCCAGCGCGGAGGCGACCCCGGCCCCG
CTGCCCCGGCGTCGGGAGGACACCGCGCGCTCACGTGGATGACT
mm4__chr2 7.95 Mbp_chr2_7950860 CAGCTGGGTAACTCAGAGGCAATCCTAGGTCTTGAAGATCCTTTCTTGGAGGCAATGAAGCTGAGGACGCCACCTCCCAG
GGCGGTGATGAGAACGAACGTGGCCACTTA
mm4__chr22 29.07 Mbp_chr22_29073669 GTACCTCCTGGGATCCCGTCCCAAGTCCCCAGCGACTTCCCCCGGGCCGGGACGTCCTCTGCTCCCCGGTACCTCCTAGG
ATC
mm4__chr16 2.93 Mbp_chr16_2932566 TAACTGGGACATGGGCTTGGGTTCGAGGAGGCCACGTGGCTCCTGACACAGCCTTAAGGATCTGAGGTGGCCCCTGCCCT
GGGCACCTGGGGTGGGAAGGGAGGTGCAGGGAACAGGGAGACTT
mm4__chr10 12.79 Mbp_chr10_12788786 ATACGCTCTTAGCAGTCGGGGCGGACGAGTCAGCTATCGCCCGGCCAGCCAAGGGCGGCCCCTCCCAGGTTGAGTTCCCT
CACTTTGTAGTTCTGTCTCTCATTTTGCTGCCGC
mm4__chr8 1.25 Mbp_chr8_1254230 CCTGAAATCAGTGCCTCCCACAGGCACCATGGTGAAGCCGGCGTCTCCCCTGCCCTGGGGGAACCCCGGGCACATCCAGG
ACTCCGCTCTGTCCCCATCATGTGACCTCTGATGGGTCCA
mm4__chr10 128.51 Mbp_chr10_128511241 AGGAAGTCCAGTGCAAGTGTTAGCAATCTTTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNCAGAGCGGGTGCGAGCTCGCCTTGGATCCCATCCACAGGACCATG
mm4__chr11 126.42 Mbp_chr11_126416868 GAATCTCGCAGGGCCTGTCGCGCTTCGTCCAGGTTGACGGCCTCCTCCCCGCGCTTTTCGGGATGCTGGCCCCGGCGCCC
CGCCTCAGGCGAGGCCGGCCCCTCCCTGGCAAGCGGCTCAAGGCTTG
mm4__chr10 102.23 Mbp_chr10_102231893 CCAAGCCAGCGCATATTCTCCGGCTCGGGGACCTCCTAAGCCACTCGCTGGCTCCCACCGGGGCTGCCCAGCCGGGGTCC
CACCCGCACTGGGGATGAAGCTGTTATGTCCTGCACCC
mm4__chr22 29.07 Mbp_chr22_29073807 GTACCTCCTGGGATCCCGTCCCCAAGTCCCCAGCGACCCCTCCCGGGCCGGGACGACCCCTGCTCCCCAGTACCTCCTGG
GATCCCGCCCCAAGTCCTTCATCGACGCA
mm4__chr7 157.82 Mbp_chr7_157824199 CAGAGCTCAGACCGTCAGTGGGGTTTGTGCACAGCCTCCTGTGACCCTCCTGAAGGTAGGAGCTGACCACAAGTCCTCCT
CCCAAGGCAGGGGCGAGCTCAGCTTGCCGACGGCTGCCAGAGAGTCACCCTCCTGA
mm3__chr11 46.38 Mbp_chr11_46384541 AATGTGACAAGGGCAGTGCTGCTGGGGCGGGGGGGACCTGGCCTCCATCATACCCACCCAGAACCTGGCTAAGTAGCTGC
AGCCAGAGGAGATTTCTGCCCCTGGAGTGGGATTTGGGGAGA
mm4__chr20 38.60 Mbp_chr20_38602052 CCTAGCCTCACCGTGTCCTCGGATCCCGCGGGCCCGCATTGCCAGGCGCTGCGCCCAGCGCTGGGCCGACCTGGGCTGCA
GGTACAGGGGTCACGGGAGCGGGAGGGAAGTTGGCCCTACTATGCACCA
mm4__chr14 100.57 Mbp_chr14_100568220 CTCCCCGCACCGAGTTACGCCCCCCGGGGCGAAGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNAGGAGGGGGACTCGGCCTGTCCCCGTTAACTCTC
mm4__chr5 135.03 Mbp_chr5_135027612 CAGGCTGAGCAGGTCAAGAATGGTGGTGGGGAAGCGGGGTAGGCAGACCTCGGCGGGGGCTGCCTCGGCGCTCTGCTCCT
ACCCAAGGGGCCCGGTTCCCTTTCTACCCACAGTTCCCTTTCTATCCGCCTCCCTCCTC
mm4__chr9 135.53 Mbp_chr9_135526339 CTGTCCTGGCCTCACTCACCCTCCCCCCCTTTCCAGGGCAGGGGACACCTTGGCTCCTCAGCAGCCCAAGGACGGCACCA
TCCAGCACCTCCGTCATT
mm4__chr3 36.76 Mbp_chr3_36764215 GAGCGCACGGTACTCACCCGGGGCAGGCCGCGCCGGGCAGGCTGGGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCTGGAGCAGAGGTG
mm4__chr6 137.22 Mbp_chr6_137219133 AACCACGGAGCCCCAGTCTCGCGACCCCACCTCGCGTCCCCGTCGCCCGCTCAGGGCCCGACGCAGGGGTCCCGGGCTAG
GGCGACCTCGGAGAAGCGGGGCGGGGCTTCCCGGCTGGGGCGGATCCCTCCCTCCCTCTC
mm4__chr4 54.23 Mbp_chr4_54226198 AAGCCAACTCAGTGGGAAGCGCCCTCAAAGCCAAAGGTGTGGCGCTCGGAGCTGTGGCCGCCCTCGCCCGGGAGTCGGGG
CCAGGAGAACGGGCCCTGGAGGCAGGGCAGGGCACGGCGAGATTTTGCTTT
mm4__chr10 101.09 Mbp_chr10_101087582 CTCTGACTCTGTTTGGGGCACCCACCACCCGTGGAAAGGAGCGGCGGCCGCCCCGGCCCAGGCTGCTGACAGCAGCAATC
CATCTCCATCACAGTAATGGCGTCCCCAT
mm4__chr8 8.70 Mbp_chr8_8701891 CTCTTTCCCTCCCCGCCCACCGCCCGCCTGGCCCGGCGGGGCGGCGCGAGGACCCAGGGGCGGGGCGACCTTTGCTCCGG
CCGTGAGCAGTCCAGGCCAG
mm4__chr6 41.10 Mbp_chr6_41101128 ACTGGTCTTTCGGAAGCCTCGGGGATGGGAACCCGAGCTCGCCACGGCCCAGGGCGTCCTCCTAGGCGTCCATGTGAC
mm4__chr9 16.19 Mbp_chr9_16192353 CTTGGGGCTTGGTCCAGATTGCGTGGCGAGGAGGGTAAGCAGGCCCAGCAGAGGGCGCCACCGCGCTGGCTCTCTTCTCC
CGCGCAGTGCAGGCAACGGGGCGGTGTTCAGGAAGGCCAAGGA
mm4__chr12 130.93 Mbp_chr12_130933852 GGATGACTGCAACCTGCTCTCATGAGTCACAGCAGGTGCCTGTCGAGACCCACACGGACCCGGGCGGGCAGGACCTCGGA
TGCTGACTCCCTGTGGGGTCAGGACCTCCTTCCTCCAGGTTCTGC
mm3__chr10 63.63 Mbp_chr10_63630244 GGAGCCACATCTGATGCACTAACCCCCGCACCCAGATGTCCAAGAGGCCGGGGGAGTCAAAGAACGCCAGGGGCGGGGCG
ACCGCGGCTGCGGCTCCTCCCTCGGGCAGAGGGAGTCCAGATGCGAG
mm4__chr2 20.64 Mbp_chr2_20638317 CACGCAGAGAGCTACTTCCCCCAACACACCAGGCCAAGGCCTCGTGGCCCAGATCCCTGCGAGGGGGCGACCTGGGATGC
TCAGGGCTTTTCTCAGATTCCAGGGAAGGGGGACGTCATCTCCAGAAA
mm4__chr1 229.43 Mbp_chr1_229431967 AGGTGGTCTCGTGAATGCCCGCCGACTCCATACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGGGGCTCACCGATGAAGGAGGGCTG
mm4__chr10 12.41 Mbp_chr10_12411172 CTGGGTGCCCACTAACCATCCCTGGGACGGGACGACCTCGACTAGCCCGCTATGTCATGTTTTCTTATTCCATGGGCATA
AGGCTTGG
mm4__chr12 121.84 Mbp_chr12_121839560 GCTTTAATCGGGAGGGCTGGAGCAGAGGGCGGCCCCGCCGAGGGGCGTGGTCAGTGTGGGCGGAGCCTTGGGGGCCGAGT
CCGCTGGTGGGCGGGACCCAAGGGGAGCAGCCAGTAGGGAAGTTGGGCGAGTTCCAGA
mm4__chr11 86.50 Mbp_chr11_86498072 TGTACAGGGCCAGCTTGCCCACAGGGATGCCCATGCCGTAGCAGCCCAGGTCTCCCAGGCCCAGGATGCGCTCCCCATCA
GTCACCACCACGGCCTGAAAAACAGCAGGGCACCATCAATCAGGGACA
mm4__chr6 154.51 Mbp_chr6_154510188 GCAGATAAAGTGCTGCTGCCTGCGCTCCGGTGCCCCTTCCCGGGAGGGCGCGCCCGCGGCTGCTCCCCTGCGCCCGAGCG
ACTCCGTCAAGACTGCATGGC
mm4__chr4 17.81 Mbp_chr4_17811348 TCACCTTCGCGACTCACTTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGGCCCAGGCCA
GAAAGCCTCCGAAGCC
mm4__chr2 74.83 Mbp_chr2_74834842 CCACGTCCGCTAAGCACAGCCGGCGAGTGCGCGCGGGCGGGGAGCCGAGGTCGCGCTCCGCCGGGCGCCCTCCCTCCCTG
AGCTCC
mm4__chr9 96.03 Mbp_chr9_96027670 ACTCCACATGCACCTTCTCCCGGGGCGCCGACGGCCTCGGCTGCCCCCGCAGCGTTTCCCTAGCTTCCCTCC
mm4__chr9 105.24 Mbp_chr9_105244658 CAGGAGCAGAGCAGGAGACGCGTAGCCGCCGTCGCCGCCGCCGGGGGATGTGGCCGGCGCCTGCCTCTAGCCGCGCCGCC
TCTTGAGTACCAG
mm4__chr8 38.90 Mbp_chr8_38901697 CAGCCCCTCCTCCAAGTGCCCCGGGTGGCCAAGGTCGCCGCCGCCAGGGGTCGGGCTGCGGGGGGTGGGGCCGCGGAGAG
GGTGGGGGCGGGAGTTCTGCTGAGCTGCATTTTCTCACGTTTGCAGCAGCGAAACTTCTC
mm4__chr5 176.81 Mbp_chr5_176810149 GAGGACTGCAGCGGAACCTGCCCGGGGCACCCCTGCCCTGGGCGGCTCTCCGCAGGCTGGCGCCGCGCCGGGGTCGCCGC
AGCCCCGGGCCCCTCCCGGGCCACCTGTGCGCAGACTG
mm4__chr20 21.71 Mbp_chr20_21709304 CAACCAGCACGGCGTGTACAGCGCCCCGGGCGGCGGCTACCTCGCCCCGGGCCCGCCGTGGCCGCCTGCGCAAGGTCCTC
CTCTGGCGCCCCCCGGGGCCGGCGTAGCTGTGCATGGCGGGGAACTCGCGGCAGCAATG
mm4__chr2 120.35 Mbp_chr2_120345217 GACGCTGCGAGACTTTTCGGCGCTCGAGCCGACCTCGCCGCCGCCGCGGCAGGCAGAAGAGACAGGAGC
mm4__chr18 79.40 Mbp_chr18_79400341 TCACGTTACGCGGAGGACGCGCGGGCAGCGCCGGGAGAACCGAACCCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNGACCCGCCATGACGGGGCTGGAGGACCAGGAGTTCGACTTCGA
mm4__chr17 74.35 Mbp_chr17_74350832 CGACAGCGAGAAGGACTCAGACACAGGTGATGACCAACCAGACATCCTGGAGCCACCCGAGGTGGCCGCAGCCCGGGAGA
GCATTGCAGCCCTGGTGGACGAGCAGAAGCAACTGCGCAAGCAGAAGGTGTCCAGGGTTT
mm4__chr1 15.85 Mbp_chr1_15847962 GGAGAGGGATGGTCTCTGCACGGGGGGGAGCCGGAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNCCGGCACCCGCCTCCCGGCGCTGACGGTCTCGTACGAAGCCG
mm3__chr9 89.43 Mbp_chr9_89429226 CTGGCTCTCCTAACCTCCCTGCCCAGTCAGCCCTCCCAGACCCACCACCAGGAGTTGTACCAGGCCGGCGGCCACCTCGG
CTGACTCTGGATGCCAAGAAGCC
mm3__chr21 43.42 Mbp_chr21_43420481 GCGGATCAGGCTCTCACAGTCTGTGGAGGGGCCAGGAGGCTGAGCCAGGGCGGCCGGGACCTCGGCTGCGCTCCCACCCA
CCGCCCCACGTGGTTTGACA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.