CRISPOR

Micro-homology scoring of potential deletions

Guide: AGGACTCGGCGCGCCGGAAG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	(Wild-type sequence)
364.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCC----------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`10 bp deleted → frameshift`
298.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGC--------------CAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`14 bp deleted → frameshift`
296.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG------CCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`6 bp deleted → no frameshift`
294.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------GGGGCGACCTCGGCTCACAGCGCGCCCGG`	`20 bp deleted → frameshift`
283.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG---------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`15 bp deleted → no frameshift`
244.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------GGGGCGACCTCGGCTCACAGCGCGCCCGG`	`18 bp deleted → no frameshift`
230.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-----------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`11 bp deleted → frameshift`
219.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG------------CGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`12 bp deleted → no frameshift`
219.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC------------CAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`12 bp deleted → no frameshift`
218.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGG`	`26 bp deleted → frameshift`
211.5	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG-------GGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`7 bp deleted → frameshift`
207.2	`CGGCGCCCCCTGGCGGCC---------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`27 bp deleted → no frameshift`
191.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGG`	`35 bp deleted → frameshift`
190.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGG-----------------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`23 bp deleted → frameshift`
180.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGG`	`24 bp deleted → no frameshift`
179.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG----------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`16 bp deleted → frameshift`
179.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------GGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`16 bp deleted → frameshift`
170.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGG`	`17 bp deleted → frameshift`
170.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGG`	`42 bp deleted → no frameshift`
165.0	`CGGCGCCCCCTGGCGG------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`36 bp deleted → no frameshift`
162.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGG`	`18 bp deleted → no frameshift`
156.1	`CGGCGCCCCCTGGC------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`30 bp deleted → no frameshift`
155.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGG`	`27 bp deleted → no frameshift`
154.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGG-------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`19 bp deleted → frameshift`
147.2	`CGGCGCCCCCTGGCGGCCTAAGG--------------------CCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`20 bp deleted → frameshift`
143.5	`CGGCGCCCCCTGGCGGCCTAAGG-------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`25 bp deleted → frameshift`
140.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGG`	`21 bp deleted → no frameshift`
133.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGG`	`22 bp deleted → frameshift`
120.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`24 bp deleted → no frameshift`
115.2	`CGGCGCCCCCTGGCGGC---------------------------------GGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`33 bp deleted → no frameshift`
114.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGG`	`25 bp deleted → frameshift`
114.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGG`	`25 bp deleted → frameshift`
110.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGG`	`20 bp deleted → frameshift`
109.2	`CGGCGCCCCCTGGCGGCCTAAGG--------------------------CGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`26 bp deleted → frameshift`
108.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGG`	`40 bp deleted → frameshift`
97.6	`CGGCGCCCCCTGGCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGG`	`42 bp deleted → no frameshift`
94.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGG`	`29 bp deleted → frameshift`
94.0	`CGGCGCCCCCTGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`29 bp deleted → frameshift`
92.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------`	`43 bp deleted → frameshift`
91.5	`CGGCGCCCCCTGGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGG`	`34 bp deleted → frameshift`
90.0	`CGGCGCC--------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`38 bp deleted → frameshift`
89.2	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGG`	`30 bp deleted → no frameshift`
89.2	`CGGCGCCCCCTGGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`30 bp deleted → no frameshift`
88.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC--------------------------------------------CCGG`	`44 bp deleted → frameshift`
85.2	`CGGCGCCCCCTGGCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGG`	`39 bp deleted → no frameshift`
84.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGG`	`31 bp deleted → frameshift`
84.8	`CGGCGCCCCCTGGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGG`	`31 bp deleted → frameshift`
81.0	`CGGC----------------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`40 bp deleted → frameshift`
80.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGG`	`32 bp deleted → frameshift`
80.8	`CGGCGCCCCCTGGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGG`	`32 bp deleted → frameshift`
80.8	`CGGCGCCCCCTGGCGG--------------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`32 bp deleted → frameshift`
80.0	`CGGCG----------------------------------------------GGGGCGACCTCGGCTCACAGCGCGCCCGG`	`46 bp deleted → frameshift`
76.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGG`	`33 bp deleted → no frameshift`
72.8	`CGGCGCCCCCTGGCGGC------------------------------------------------TCACAGCGCGCCCGG`	`48 bp deleted → no frameshift`
69.6	`CGGCGCCCCCTGG-----------------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`35 bp deleted → frameshift`
69.6	`CGGCGCCCCC-----------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`35 bp deleted → frameshift`
67.5	`CGGCGCCCCCTGGCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGG`	`40 bp deleted → frameshift`
66.0	`CGGCGCCCC------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`36 bp deleted → no frameshift`
63.6	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGG`	`31 bp deleted → frameshift`
62.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGG`	`37 bp deleted → frameshift`
62.8	`CGGCGCCCCCTGGCGG-------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`37 bp deleted → frameshift`
62.8	`CGGCGCCC-------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`37 bp deleted → frameshift`
61.0	`CGGCGCCCCCTGGCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGG`	`42 bp deleted → no frameshift`
60.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGG`	`38 bp deleted → frameshift`
60.0	`CGGCGCCCCCTGGCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGG`	`38 bp deleted → frameshift`
59.2	`CGGCG----------------------------------------------------ACCTCGGCTCACAGCGCGCCCGG`	`52 bp deleted → frameshift`
56.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGG`	`39 bp deleted → no frameshift`
56.8	`CGGCGCCCCCTGG---------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`39 bp deleted → no frameshift`
54.0	`CGGCGCCCCCTGG----------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`40 bp deleted → frameshift`
51.6	`CGGCGCCCCCTGGCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGG`	`41 bp deleted → frameshift`
51.6	`CGGCGCCCCCTGG-----------------------------------------GCGACCTCGGCTCACAGCGCGCCCGG`	`41 bp deleted → frameshift`
51.6	`CGG-------------------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGG`	`49 bp deleted → frameshift`
46.8	`CGGCGCCCCCTGGCGGCCTAAGGACTCGG---------------------------------------------------`	`51 bp deleted → no frameshift`
46.8	`CGGCGCCCCCTGGC---------------------------------------------------TCACAGCGCGCCCGG`	`51 bp deleted → no frameshift`
44.4	`CGGCGC--------------------------------------------GGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`44 bp deleted → frameshift`
42.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCG---------------------------------------------CGCCCGG`	`45 bp deleted → no frameshift`
42.0	`CGG---------------------------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGG`	`45 bp deleted → no frameshift`
41.0	`CGGCGCCCCCT--------------------------------------------------CGGCTCACAGCGCGCCCGG`	`50 bp deleted → frameshift`
40.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG----------------------------------------------G`	`46 bp deleted → frameshift`
40.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGC----------------------------------------------CCGG`	`46 bp deleted → frameshift`
38.0	`CGGCGCCCCCTGGCGGCCTAAGGACTCG-----------------------------------------------CCCGG`	`47 bp deleted → frameshift`
37.6	`CGGC-------------------------------------------------------------TCACAGCGCGCCCGG`	`61 bp deleted → frameshift`
36.4	`CGGCGCCCCCTGGCGGCCTAAGGACTCGGCG------------------------------------------------G`	`48 bp deleted → no frameshift`
36.0	`CGGCGCCC----------------------------------------------------------------------GG`	`70 bp deleted → frameshift`
33.3	`CGGCGCCCCCTGGCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGG`	`44 bp deleted → frameshift`
33.0	`CGGCGCCCCCTGGCG----------------------------------------------------------CGCCCGG`	`58 bp deleted → frameshift`
32.8	`CGGCGC--------------------------------------------------GACCTCGGCTCACAGCGCGCCCGG`	`50 bp deleted → frameshift`
32.8	`CGG--------------------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGG`	`50 bp deleted → frameshift`
31.2	`CGGCGCCCCCTGGCGGCC-----------------------------------------------------------CGG`	`59 bp deleted → frameshift`
31.2	`CGGCGCCCC---------------------------------------------------TCGGCTCACAGCGCGCCCGG`	`51 bp deleted → no frameshift`
31.2	`CGG---------------------------------------------------GCGACCTCGGCTCACAGCGCGCCCGG`	`51 bp deleted → no frameshift`
30.0	`CGGCGCCCCCTGGCG------------------------------------------------------------CCCGG`	`60 bp deleted → no frameshift`
29.6	`CGGCGCCC----------------------------------------------------TCGGCTCACAGCGCGCCCGG`	`52 bp deleted → frameshift`
28.5	`CGGCGCCCCCTGGCGGCCT-----------------------------------------------CACAGCGCGCCCGG`	`47 bp deleted → frameshift`
28.4	`CGGCGCC-----------------------------------------------------TCGGCTCACAGCGCGCCCGG`	`53 bp deleted → frameshift`
26.4	`CGGCGC--------------------------------------------------------------------GCCCGG`	`68 bp deleted → frameshift`
25.8	`CGGCGCCCCCTGGCGGCCTAAG-------------------------------------------------CGCGCCCGG`	`49 bp deleted → frameshift`
25.6	`CGGCGCCCCCTGGCGGC-------------------------------------------------------GCGCCCGG`	`55 bp deleted → frameshift`
25.6	`CG-------------------------------------------------------ACCTCGGCTCACAGCGCGCCCGG`	`55 bp deleted → frameshift`
24.6	`CGGCGCCCCCTGGCGG----------------------------------------------------------------`	`64 bp deleted → frameshift`
23.2	`CGGCGCCCCCTGGCGGCCTAAGG---------------------------------------------------------`	`57 bp deleted → no frameshift`
23.2	`CGGCGCCCCCTGGCGGC---------------------------------------------------------GCCCGG`	`57 bp deleted → no frameshift`
22.0	`CGGCG----------------------------------------------------------GCTCACAGCGCGCCCGG`	`58 bp deleted → frameshift`
20.8	`CGGCGC-----------------------------------------------------------TCACAGCGCGCCCGG`	`59 bp deleted → frameshift`
20.0	`CGGCGCCCCCTGGCGGCC------------------------------------------------------------GG`	`60 bp deleted → no frameshift`
19.8	`CGGCGCCCCC--------------------------------------------------------------------GG`	`68 bp deleted → frameshift`
19.2	`CGGCGCCCCCT-------------------------------------------------------CACAGCGCGCCCGG`	`55 bp deleted → frameshift`
19.2	`CGGCGCCCC---------------------------------------------------------------------GG`	`69 bp deleted → no frameshift`
18.0	`CGGCGCCCCCTGGC--------------------------------------------------------------CCGG`	`62 bp deleted → frameshift`
14.8	`CGGCGC------------------------------------------------------------------GCGCCCGG`	`66 bp deleted → no frameshift`
14.0	`CGGCGCCCCCTGG-------------------------------------------------------------------`	`67 bp deleted → frameshift`
14.0	`CGGCGCCCCC-------------------------------------------------------------------CGG`	`67 bp deleted → frameshift`
12.6	`CGG-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`
11.6	`CGGCGCC-----------------------------------------------------------------------GG`	`71 bp deleted → frameshift`
11.6	`CG-----------------------------------------------------------------------CGCCCGG`	`71 bp deleted → frameshift`
10.8	`CGGC------------------------------------------------------------------------CCGG`	`72 bp deleted → no frameshift`
10.4	`CG-------------------------------------------------------------------------CCCGG`	`73 bp deleted → frameshift`
10.0	`CGGCG--------------------------------------------------------------------------G`	`74 bp deleted → frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License