CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCGCCGGAAGTGGCCAGGG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	(Wild-type sequence)
491.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG----GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`4 bp deleted → frameshift`
467.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG-----GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`5 bp deleted → frameshift`
444.6	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`6 bp deleted → no frameshift`
344.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG---GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`3 bp deleted → no frameshift`
329.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`12 bp deleted → no frameshift`
283.2	`CTGGCGGCCTAAGGACTCGGCGCGCCGG---------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`15 bp deleted → no frameshift`
282.0	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG-------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`7 bp deleted → frameshift`
255.2	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG---------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`9 bp deleted → no frameshift`
242.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG----------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`10 bp deleted → frameshift`
230.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`11 bp deleted → frameshift`
218.4	`CTGGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`26 bp deleted → frameshift`
210.0	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTCTCG`	`21 bp deleted → no frameshift`
191.4	`CTGGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTCTCG`	`35 bp deleted → frameshift`
190.2	`CTGGCGGCCTAAGGACTCGG-----------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`23 bp deleted → frameshift`
188.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG---------------CTCACAGCGCGCCCGGCTATTCTCG`	`15 bp deleted → no frameshift`
188.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------TCGGCTCACAGCGCGCCCGGCTATTCTCG`	`15 bp deleted → no frameshift`
180.6	`CTGGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`24 bp deleted → no frameshift`
179.6	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG----------------CTCACAGCGCGCCCGGCTATTCTCG`	`16 bp deleted → frameshift`
179.6	`CTGGCGGCCTAAGGACTCGGCGCGCCGG----------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`16 bp deleted → frameshift`
170.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`17 bp deleted → frameshift`
170.8	`CTGGCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGGCTATTCTCG`	`42 bp deleted → no frameshift`
162.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`18 bp deleted → no frameshift`
162.8	`CTGGCGGCCTAAGGACTCGGCGCG------------------GGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`18 bp deleted → no frameshift`
155.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTCTCG`	`27 bp deleted → no frameshift`
150.5	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAG------------------------CGCGCCCGGCTATTCTCG`	`24 bp deleted → no frameshift`
147.2	`CTGGCGGCCTAAGGACTCGGCG--------------------GGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`20 bp deleted → frameshift`
140.0	`CTGGCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`21 bp deleted → no frameshift`
133.2	`CTGGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`22 bp deleted → frameshift`
121.2	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTCTCG`	`32 bp deleted → frameshift`
120.4	`CTGGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`24 bp deleted → no frameshift`
114.8	`CTGGCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGGCTATTCTCG`	`25 bp deleted → frameshift`
114.8	`CTGGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`25 bp deleted → frameshift`
110.4	`CTGGCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`20 bp deleted → frameshift`
108.0	`CTGGCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGGCTATTCTCG`	`40 bp deleted → frameshift`
99.9	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTCTCG`	`22 bp deleted → frameshift`
99.0	`CTGGCGG------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`36 bp deleted → no frameshift`
98.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTCTCG`	`28 bp deleted → frameshift`
97.6	`CTGGCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`42 bp deleted → no frameshift`
94.2	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTCTCG`	`37 bp deleted → frameshift`
94.0	`CTGGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`29 bp deleted → frameshift`
94.0	`CTGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`29 bp deleted → frameshift`
92.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------CTATTCTCG`	`43 bp deleted → frameshift`
91.5	`CTGGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`34 bp deleted → frameshift`
89.2	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTCTCG`	`30 bp deleted → no frameshift`
89.2	`CTGGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTCTCG`	`30 bp deleted → no frameshift`
89.2	`CTGGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`30 bp deleted → no frameshift`
88.8	`CTGGCGGCCTAAGGACTCGGCGC--------------------------------------------CCGGCTATTCTCG`	`44 bp deleted → frameshift`
85.2	`CTGGCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`39 bp deleted → no frameshift`
84.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG-------------------------------CTATTCTCG`	`31 bp deleted → frameshift`
84.8	`CTGGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTCTCG`	`31 bp deleted → frameshift`
84.8	`CTGGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`31 bp deleted → frameshift`
80.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG--------------------------------CTATTCTCG`	`32 bp deleted → frameshift`
80.8	`CTGGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTCTCG`	`32 bp deleted → frameshift`
80.8	`CTGGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`32 bp deleted → frameshift`
76.8	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTCTCG`	`33 bp deleted → no frameshift`
76.8	`CTGGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTCTCG`	`33 bp deleted → no frameshift`
72.8	`CTGGCGGC------------------------------------------------TCACAGCGCGCCCGGCTATTCTCG`	`48 bp deleted → no frameshift`
67.5	`CTGGCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGGCTATTCTCG`	`40 bp deleted → frameshift`
63.6	`CTGGCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTCTCG`	`31 bp deleted → frameshift`
62.8	`CTGGCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTCTCG`	`37 bp deleted → frameshift`
62.8	`CTGGCGG-------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`37 bp deleted → frameshift`
62.4	`CTGGCGGCCTAAGGACTCGGC---------------------------------------------------TATTCTCG`	`51 bp deleted → no frameshift`
61.0	`CTGGCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGGCTATTCTCG`	`42 bp deleted → no frameshift`
60.0	`CTGGCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTCTCG`	`38 bp deleted → frameshift`
60.0	`CTGGCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`38 bp deleted → frameshift`
56.8	`CTGGCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGGCTATTCTCG`	`39 bp deleted → no frameshift`
56.8	`CTGG---------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`39 bp deleted → no frameshift`
54.0	`CTGG----------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`40 bp deleted → frameshift`
51.6	`CTGGCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGGCTATTCTCG`	`41 bp deleted → frameshift`
51.6	`CTGG-----------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCG`	`41 bp deleted → frameshift`
46.8	`CTGGC---------------------------------------------------TCACAGCGCGCCCGGCTATTCTCG`	`51 bp deleted → no frameshift`
42.0	`CTGGCGGCCTAAGGACTCG---------------------------------------------CGCCCGGCTATTCTCG`	`45 bp deleted → no frameshift`
40.0	`CTGGCGGCCTAAGGACTCGGCGCG----------------------------------------------GCTATTCTCG`	`46 bp deleted → frameshift`
40.0	`CTGGCGGCCTAAGGACTCGGC----------------------------------------------CCGGCTATTCTCG`	`46 bp deleted → frameshift`
38.0	`CTGGCGGCCTAAGGACTCGGCGCGC-----------------------------------------------TATTCTCG`	`47 bp deleted → frameshift`
38.0	`CTGGCGGCCTAAGGACTCG-----------------------------------------------CCCGGCTATTCTCG`	`47 bp deleted → frameshift`
36.4	`CTGGCGGCCTAAGGACTCGGCG------------------------------------------------GCTATTCTCG`	`48 bp deleted → no frameshift`
34.4	`CTGGCGGCCTAAGGACTCGGCGC-------------------------------------------------TATTCTCG`	`49 bp deleted → frameshift`
33.3	`CTGGCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGGCTATTCTCG`	`44 bp deleted → frameshift`
33.0	`CTGGCG----------------------------------------------------------CGCCCGGCTATTCTCG`	`58 bp deleted → frameshift`
32.9	`CTGGCGGCCTAAGGACTCG-------------------------------------------------------------`	`61 bp deleted → frameshift`
32.8	`CTGGCGGC----------------------------------------------------------------TATTCTCG`	`64 bp deleted → frameshift`
31.2	`CTGGCGGCC-----------------------------------------------------------CGGCTATTCTCG`	`59 bp deleted → frameshift`
30.0	`CTGGCG------------------------------------------------------------CCCGGCTATTCTCG`	`60 bp deleted → no frameshift`
28.5	`CTGGCGGCCT-----------------------------------------------CACAGCGCGCCCGGCTATTCTCG`	`47 bp deleted → frameshift`
28.4	`CTGGCGGCCTAAGGACTCGGCGCGCCG-----------------------------------------------------`	`53 bp deleted → frameshift`
25.8	`CTGGCGGCCTAAG-------------------------------------------------CGCGCCCGGCTATTCTCG`	`49 bp deleted → frameshift`
25.6	`CTGGCGGC-------------------------------------------------------GCGCCCGGCTATTCTCG`	`55 bp deleted → frameshift`
24.6	`CT--------------------------------------------------CGGCTCACAGCGCGCCCGGCTATTCTCG`	`50 bp deleted → frameshift`
24.4	`CTGGCGGCCTAAGGACTCGGCGCG--------------------------------------------------------`	`56 bp deleted → frameshift`
23.2	`CTGGCGGCCTAAGG---------------------------------------------------------CTATTCTCG`	`57 bp deleted → no frameshift`
23.2	`CTGGCGGC---------------------------------------------------------GCCCGGCTATTCTCG`	`57 bp deleted → no frameshift`
22.0	`CTGGCGGCCTAAGGACTCGGCG----------------------------------------------------------`	`58 bp deleted → frameshift`
21.0	`CTGGC-------------------------------------------------------------------TATTCTCG`	`67 bp deleted → frameshift`
20.0	`CTGGCGGCC------------------------------------------------------------GGCTATTCTCG`	`60 bp deleted → no frameshift`
19.2	`CT-------------------------------------------------------CACAGCGCGCCCGGCTATTCTCG`	`55 bp deleted → frameshift`
18.3	`CTGGCGGCCTAAGGACT--------------------------------------------------------ATTCTCG`	`56 bp deleted → frameshift`
18.0	`CTGGC--------------------------------------------------------------CCGGCTATTCTCG`	`62 bp deleted → frameshift`
17.2	`CTGGCGGCCTA---------------------------------------------------------------TTCTCG`	`63 bp deleted → no frameshift`
15.6	`CTGGCGGCCTAAGGACTC-----------------------------------------------------------TCG`	`59 bp deleted → frameshift`
10.0	`CTGGCG--------------------------------------------------------------------------`	`74 bp deleted → frameshift`
9.9	`CTGGCGGCCT--------------------------------------------------------------------CG`	`68 bp deleted → frameshift`
8.7	`CT-----------------------------------------------------------------------ATTCTCG`	`71 bp deleted → frameshift`
6.6	`CT----------------------------------------------------------------------------CG`	`76 bp deleted → frameshift`

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