CRISPOR

Micro-homology scoring of potential deletions

Guide: CGGCGCCCCCTGGCGGCCTA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	(Wild-type sequence)
417.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC-------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`13 bp deleted → frameshift`
359.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCG----------------CGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`16 bp deleted → frameshift`
329.4	`TCCCCAATCTGGGCGCGCGCCGG----------------------------------AAGTGGCCAGGGCGGGGGCGACC`	`34 bp deleted → frameshift`
327.6	`TCCCCAATCTGGGCGCGCGCCGGCGC--------------------------GCCGGAAGTGGCCAGGGCGGGGGCGACC`	`26 bp deleted → frameshift`
282.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG-------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`7 bp deleted → frameshift`
256.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCC-----------------GGAAGTGGCCAGGGCGGGGGCGACC`	`17 bp deleted → frameshift`
247.0	`TCCCCAATCTGGGCGCGCGCCGGCGCC----------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`28 bp deleted → frameshift`
244.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCG------------------CCGGAAGTGGCCAGGGCGGGGGCGACC`	`18 bp deleted → no frameshift`
242.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG----------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`10 bp deleted → frameshift`
231.0	`TCCCCAATCTGGGCGCGC------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACC`	`36 bp deleted → no frameshift`
211.5	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCT-------CGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`7 bp deleted → frameshift`
210.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG---------------------AAGTGGCCAGGGCGGGGGCGACC`	`21 bp deleted → no frameshift`
207.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCC---------------------------AGGGCGGGGGCGACC`	`27 bp deleted → no frameshift`
188.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------GCCGGAAGTGGCCAGGGCGGGGGCGACC`	`15 bp deleted → no frameshift`
165.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG------------------------------------GGGCGACC`	`36 bp deleted → no frameshift`
161.6	`TCCCCAATCTGGGCGCGCGC--------------------------------GCCGGAAGTGGCCAGGGCGGGGGCGACC`	`32 bp deleted → frameshift`
156.1	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGC------------------------------CAGGGCGGGGGCGACC`	`30 bp deleted → no frameshift`
147.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGC--------------------CGGAAGTGGCCAGGGCGGGGGCGACC`	`20 bp deleted → frameshift`
147.2	`TCCCCAATCTGGGCGCGCGCCGG--------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`20 bp deleted → frameshift`
141.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCT---------------CGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`15 bp deleted → no frameshift`
126.8	`TCCCCAATCTGGGCGCGCGCCGGCG-----------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`23 bp deleted → frameshift`
120.4	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG------------------------AAGTGGCCAGGGCGGGGGCGACC`	`24 bp deleted → no frameshift`
120.4	`TCCCCAATCTGGGCGCGCGCCGGCGC------------------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`24 bp deleted → no frameshift`
120.0	`TCCCCAATCTGGGCGC--------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACC`	`38 bp deleted → frameshift`
115.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------------------------GGGGGCGACC`	`33 bp deleted → no frameshift`
114.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCC-------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`25 bp deleted → frameshift`
109.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCC--------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`26 bp deleted → frameshift`
103.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCC---------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`27 bp deleted → no frameshift`
97.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCG------------------------------------------ACC`	`42 bp deleted → no frameshift`
94.0	`TCCCCAATCTGGGCGCGCGCCG-----------------------------CGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`29 bp deleted → frameshift`
94.0	`TCCCCAATCTGGGCGCGCG-----------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`29 bp deleted → frameshift`
90.0	`TCCCCAATCTGGGCGCGCGCCGGCGCC--------------------------------------AGGGCGGGGGCGACC`	`38 bp deleted → frameshift`
89.2	`TCCCCAATCTGGGCGCGCGCCGGC------------------------------CGGAAGTGGCCAGGGCGGGGGCGACC`	`30 bp deleted → no frameshift`
89.2	`TCCCCAATCTGGGCGCGCGC------------------------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`30 bp deleted → no frameshift`
89.2	`TCCCCAATCTGGG------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`30 bp deleted → no frameshift`
85.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------------------------------GACC`	`39 bp deleted → no frameshift`
84.8	`TCCCCAATCTGGGCGCGCGCCGGCG-------------------------------GAAGTGGCCAGGGCGGGGGCGACC`	`31 bp deleted → frameshift`
84.8	`TCCCCAATCTGGGCGCGCGCCG-------------------------------CCGGAAGTGGCCAGGGCGGGGGCGACC`	`31 bp deleted → frameshift`
84.8	`TCCCCAATCTGGGCGCG-------------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`31 bp deleted → frameshift`
84.8	`TCCCCAATCTGG-------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`31 bp deleted → frameshift`
81.0	`TCCCCAATCTGGGCGCGCGCCGGC----------------------------------------CAGGGCGGGGGCGACC`	`40 bp deleted → frameshift`
80.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG--------------------------------GCGGGGGCGACC`	`32 bp deleted → frameshift`
80.0	`TCCCCAATCTGGGCGCGCGCCGGCG----------------------------------------------GGGGCGACC`	`46 bp deleted → frameshift`
76.8	`TCCCCAATCTGGGCG---------------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`33 bp deleted → no frameshift`
69.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG-----------------------------------GCGGGGGCGACC`	`35 bp deleted → frameshift`
69.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCC-----------------------------------AGGGCGGGGGCGACC`	`35 bp deleted → frameshift`
66.6	`TCCCCAATCTGGGCGCGCGCC--------------------------------------------AGGGCGGGGGCGACC`	`44 bp deleted → frameshift`
66.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCC------------------------------------AGGGCGGGGGCGACC`	`36 bp deleted → no frameshift`
62.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG-------------------------------------GGCGACC`	`37 bp deleted → frameshift`
62.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCC-------------------------------------AGGGCGGGGGCGACC`	`37 bp deleted → frameshift`
62.8	`TCCCCAATCTGGGCGCGCG-------------------------------------GAAGTGGCCAGGGCGGGGGCGACC`	`37 bp deleted → frameshift`
62.8	`TCCCCAATCTGG-------------------------------------CGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`37 bp deleted → frameshift`
61.0	`TCCCCAATCTGGGCG--------------------------------------------------------GGGGCGACC`	`56 bp deleted → frameshift`
60.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGG--------------------------------------GCGACC`	`38 bp deleted → frameshift`
59.2	`TCCCCAATCTGGGCGCGCGCCGGCG----------------------------------------------------ACC`	`52 bp deleted → frameshift`
56.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG---------------------------------------GGGCGACC`	`39 bp deleted → no frameshift`
56.8	`TCCCCAATCTGGGCGCG---------------------------------------GAAGTGGCCAGGGCGGGGGCGACC`	`39 bp deleted → no frameshift`
54.0	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG----------------------------------------GGCGACC`	`40 bp deleted → frameshift`
54.0	`TCCCCAATCTGGGC----------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACC`	`40 bp deleted → frameshift`
51.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGG-----------------------------------------GCGACC`	`41 bp deleted → frameshift`
51.6	`TCCCCAATCTGGGCG-----------------------------------------GAAGTGGCCAGGGCGGGGGCGACC`	`41 bp deleted → frameshift`
51.6	`TCCCCAATCTGGGCGCGCGCCGG-------------------------------------------------GGGCGACC`	`49 bp deleted → frameshift`
49.5	`TCCCCAATCT------------------------------------CGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`36 bp deleted → no frameshift`
49.2	`TCCCCAATCTGGGC--------------------------------------------------CAGGGCGGGGGCGACC`	`50 bp deleted → frameshift`
48.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCC------------------------------------------`	`42 bp deleted → no frameshift`
45.0	`TCCCCAATCTGGGCG--------------------------------------------------------------ACC`	`62 bp deleted → frameshift`
45.0	`TCCCCAATC--------------------------------------GGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`38 bp deleted → frameshift`
44.4	`TCCCCAATCTGGGCGCGCGCCGGCGC--------------------------------------------GGGGGCGACC`	`44 bp deleted → frameshift`
44.4	`TCCCCAATCTGGGCGCGCG----------------------------------------------------GGGGCGACC`	`52 bp deleted → frameshift`
44.4	`TCCCCAATCTGGG--------------------------------------------AAGTGGCCAGGGCGGGGGCGACC`	`44 bp deleted → frameshift`
42.0	`TCCCCAATCTGGGCGCGCGCCGG---------------------------------------------GCGGGGGCGACC`	`45 bp deleted → no frameshift`
42.0	`TCCCCAATCTGG---------------------------------------------AAGTGGCCAGGGCGGGGGCGACC`	`45 bp deleted → no frameshift`
40.2	`TCCCCAATCTGGGCGCG------------------------------------------------------GGGGCGACC`	`54 bp deleted → no frameshift`
40.0	`TCCCCAATCTGGGCGCGC----------------------------------------------CAGGGCGGGGGCGACC`	`46 bp deleted → frameshift`
39.0	`TCCCCAATCTGG---------------------------------------------------CCAGGGCGGGGGCGACC`	`51 bp deleted → no frameshift`
36.4	`TCCCCAATCTGGGCGC------------------------------------------------CAGGGCGGGGGCGACC`	`48 bp deleted → no frameshift`
33.0	`TCCCCAATCTGGGCGCGCG----------------------------------------------------------ACC`	`58 bp deleted → frameshift`
32.8	`TCCCCAATCTGGGCGCGCGCCGGCGCCCCC--------------------------------------------------`	`50 bp deleted → frameshift`
32.8	`TCCCCAATCTGGGCGCGCGCCGGCGC--------------------------------------------------GACC`	`50 bp deleted → frameshift`
32.8	`TCCCCAATCTGGGCGCGCGCCGG--------------------------------------------------GGCGACC`	`50 bp deleted → frameshift`
32.8	`TCCCCAATCTGGGCGCGCGC--------------------------------------------------GGGGGCGACC`	`50 bp deleted → frameshift`
32.8	`TCCCC--------------------------------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`50 bp deleted → frameshift`
31.5	`TC---------------------------------------------GGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC`	`45 bp deleted → no frameshift`
31.2	`TCCCCAATCTGGGCGCGCGCCGGCGCCCC---------------------------------------------------`	`51 bp deleted → no frameshift`
31.2	`TCCCCAATCTGGGCGCGCGCCGG---------------------------------------------------GCGACC`	`51 bp deleted → no frameshift`
31.2	`TCCC---------------------------------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`51 bp deleted → no frameshift`
30.0	`TCCCCAATCTGGGCGCG------------------------------------------------------------ACC`	`60 bp deleted → no frameshift`
30.0	`TCCCCAATCTGGG------------------------------------------------------------GGCGACC`	`60 bp deleted → no frameshift`
29.6	`TCCCCAATCTGGGCGCGCGCCGGCGCCC----------------------------------------------------`	`52 bp deleted → frameshift`
29.6	`TCC----------------------------------------------------GGAAGTGGCCAGGGCGGGGGCGACC`	`52 bp deleted → frameshift`
28.4	`TCCCCAATCTGGGCGCGCGCCGGCGCC-----------------------------------------------------`	`53 bp deleted → frameshift`
28.2	`TCCCCAATCTGGG-------------------------------------------------------------GCGACC`	`61 bp deleted → frameshift`
25.6	`TCCCCAATCTGGGCGCGCGCCG-------------------------------------------------------ACC`	`55 bp deleted → frameshift`
25.6	`TCCCCAATCTGGG-------------------------------------------------------GCGGGGGCGACC`	`55 bp deleted → frameshift`
25.0	`TCCCCA------------------------------------------------------------GGGCGGGGGCGACC`	`60 bp deleted → no frameshift`
24.4	`TCCCCAATCTGGGCGCGCGC--------------------------------------------------------GACC`	`56 bp deleted → frameshift`
23.2	`TCCCCAATCTGG---------------------------------------------------------CGGGGGCGACC`	`57 bp deleted → no frameshift`
20.8	`TCCCCAATCTGGGCGCGCGCC-----------------------------------------------------------`	`59 bp deleted → frameshift`
20.8	`TCCCCAATCTGGG-----------------------------------------------------------GGGCGACC`	`59 bp deleted → frameshift`
18.8	`TCCC-------------------------------------------------------------AGGGCGGGGGCGACC`	`61 bp deleted → frameshift`
18.0	`TCC--------------------------------------------------------------AGGGCGGGGGCGACC`	`62 bp deleted → frameshift`
17.2	`TCCCCAATCTGG---------------------------------------------------------------CGACC`	`63 bp deleted → no frameshift`
14.8	`TCCCCAA----------------------------------------------------GTGGCCAGGGCGGGGGCGACC`	`52 bp deleted → frameshift`
9.6	`TCCCC---------------------------------------------------------------------------`	`75 bp deleted → no frameshift`
8.8	`TCCC----------------------------------------------------------------------------`	`76 bp deleted → frameshift`
8.4	`TCC-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`

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