CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCCGGAAGTGGCCAGGGCG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	(Wild-type sequence)
741.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`6 bp deleted → no frameshift`
467.4	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG-----GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`5 bp deleted → frameshift`
329.4	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`12 bp deleted → no frameshift`
327.6	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG----GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`4 bp deleted → frameshift`
283.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC---------------TCACAGCGCGCCCGGCTATTCTCGCA`	`15 bp deleted → no frameshift`
282.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG-------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`7 bp deleted → frameshift`
242.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG----------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`10 bp deleted → frameshift`
230.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`11 bp deleted → frameshift`
219.6	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG------------GCTCACAGCGCGCCCGGCTATTCTCGCA`	`12 bp deleted → no frameshift`
218.4	`GGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`26 bp deleted → frameshift`
210.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`21 bp deleted → no frameshift`
199.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG----------------------CGCCCGGCTATTCTCGCA`	`22 bp deleted → frameshift`
191.4	`GGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`35 bp deleted → frameshift`
188.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------TCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`15 bp deleted → no frameshift`
180.6	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG------------------------CCCGGCTATTCTCGCA`	`24 bp deleted → no frameshift`
180.6	`GGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`24 bp deleted → no frameshift`
179.6	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG----------------CTCACAGCGCGCCCGGCTATTCTCGCA`	`16 bp deleted → frameshift`
179.6	`GGCGGCCTAAGGACTCGGCGCGCCGG----------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`16 bp deleted → frameshift`
170.8	`GGCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`17 bp deleted → frameshift`
170.8	`GGCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGGCTATTCTCGCA`	`42 bp deleted → no frameshift`
162.8	`GGCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`18 bp deleted → no frameshift`
155.4	`GGCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTCTCGCA`	`27 bp deleted → no frameshift`
150.5	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAG------------------------CGCGCCCGGCTATTCTCGCA`	`24 bp deleted → no frameshift`
140.0	`GGCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`21 bp deleted → no frameshift`
133.2	`GGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`22 bp deleted → frameshift`
127.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC-------------------------------TATTCTCGCA`	`31 bp deleted → frameshift`
121.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTCTCGCA`	`32 bp deleted → frameshift`
120.4	`GGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`24 bp deleted → no frameshift`
114.8	`GGCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`25 bp deleted → frameshift`
114.8	`GGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`25 bp deleted → frameshift`
110.4	`GGCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`20 bp deleted → frameshift`
109.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC--------------------------CCGGCTATTCTCGCA`	`26 bp deleted → frameshift`
108.0	`GGCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGGCTATTCTCGCA`	`40 bp deleted → frameshift`
99.9	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTCTCGCA`	`22 bp deleted → frameshift`
98.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG----------------------------GCTATTCTCGCA`	`28 bp deleted → frameshift`
98.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTCTCGCA`	`28 bp deleted → frameshift`
97.6	`GGCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`42 bp deleted → no frameshift`
94.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTCTCGCA`	`37 bp deleted → frameshift`
94.0	`GGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`29 bp deleted → frameshift`
92.8	`GGCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------CTATTCTCGCA`	`43 bp deleted → frameshift`
91.5	`GGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`34 bp deleted → frameshift`
89.2	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTCTCGCA`	`30 bp deleted → no frameshift`
89.2	`GGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCA`	`30 bp deleted → no frameshift`
89.2	`GGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`30 bp deleted → no frameshift`
88.8	`GGCGGCCTAAGGACTCGGCGC--------------------------------------------CCGGCTATTCTCGCA`	`44 bp deleted → frameshift`
85.2	`GGCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`39 bp deleted → no frameshift`
84.8	`GGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`31 bp deleted → frameshift`
84.8	`GGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`31 bp deleted → frameshift`
80.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG--------------------------------CTATTCTCGCA`	`32 bp deleted → frameshift`
80.8	`GGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCA`	`32 bp deleted → frameshift`
80.8	`GGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`32 bp deleted → frameshift`
76.8	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTCTCGCA`	`33 bp deleted → no frameshift`
76.8	`GGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`33 bp deleted → no frameshift`
72.8	`GGCGGC------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`48 bp deleted → no frameshift`
67.5	`GGCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGGCTATTCTCGCA`	`40 bp deleted → frameshift`
63.6	`GGCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTCTCGCA`	`31 bp deleted → frameshift`
62.8	`GGCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTCTCGCA`	`37 bp deleted → frameshift`
62.8	`GGCGG-------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`37 bp deleted → frameshift`
62.4	`GGCGGCCTAAGGACTCGGC---------------------------------------------------TATTCTCGCA`	`51 bp deleted → no frameshift`
61.0	`GGCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`42 bp deleted → no frameshift`
60.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG--------------------------------------CA`	`38 bp deleted → frameshift`
60.0	`GGCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTCTCGCA`	`38 bp deleted → frameshift`
60.0	`GGCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`38 bp deleted → frameshift`
56.8	`GGCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGGCTATTCTCGCA`	`39 bp deleted → no frameshift`
54.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC----------------------------------------A`	`40 bp deleted → frameshift`
54.0	`GG----------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`40 bp deleted → frameshift`
51.6	`GGCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGGCTATTCTCGCA`	`41 bp deleted → frameshift`
51.6	`GG-----------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCA`	`41 bp deleted → frameshift`
46.8	`GGC---------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGCA`	`51 bp deleted → no frameshift`
42.0	`GGCGGCCTAAGGACTCG---------------------------------------------CGCCCGGCTATTCTCGCA`	`45 bp deleted → no frameshift`
40.0	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------------------------A`	`46 bp deleted → frameshift`
40.0	`GGCGGCCTAAGGACTCGGCGCG----------------------------------------------GCTATTCTCGCA`	`46 bp deleted → frameshift`
40.0	`GGCGGCCTAAGGACTCGGC----------------------------------------------CCGGCTATTCTCGCA`	`46 bp deleted → frameshift`
38.0	`GGCGGCCTAAGGACTCGGCGCGC-----------------------------------------------TATTCTCGCA`	`47 bp deleted → frameshift`
38.0	`GGCGGCCTAAGGACTCG-----------------------------------------------CCCGGCTATTCTCGCA`	`47 bp deleted → frameshift`
36.6	`GGCGGCCTAAGGACTCGGCGCGC--------------------------------------------------------A`	`56 bp deleted → frameshift`
36.4	`GGCGGCCTAAGGACTCGGCG------------------------------------------------GCTATTCTCGCA`	`48 bp deleted → no frameshift`
34.4	`GGCGGCCTAAGGACTCGGCGC-------------------------------------------------TATTCTCGCA`	`49 bp deleted → frameshift`
33.3	`GGCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGGCTATTCTCGCA`	`44 bp deleted → frameshift`
33.0	`GGCGGCCTAAGGACTCGGCGC----------------------------------------------------------A`	`58 bp deleted → frameshift`
33.0	`GGCG----------------------------------------------------------CGCCCGGCTATTCTCGCA`	`58 bp deleted → frameshift`
32.9	`GGCGGCCTAAGGACTCG-------------------------------------------------------------CA`	`61 bp deleted → frameshift`
32.8	`GGCGGC----------------------------------------------------------------TATTCTCGCA`	`64 bp deleted → frameshift`
31.5	`GGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA---------------------------------------------`	`45 bp deleted → no frameshift`
31.2	`GGCGGCC-----------------------------------------------------------CGGCTATTCTCGCA`	`59 bp deleted → frameshift`
30.0	`GGCG------------------------------------------------------------CCCGGCTATTCTCGCA`	`60 bp deleted → no frameshift`
28.5	`GGCGGCCT-----------------------------------------------CACAGCGCGCCCGGCTATTCTCGCA`	`47 bp deleted → frameshift`
28.4	`GGCGGCCTAAGGACTCGGCGCGCCG-----------------------------------------------------CA`	`53 bp deleted → frameshift`
25.8	`GGCGGCCTAAG-------------------------------------------------CGCGCCCGGCTATTCTCGCA`	`49 bp deleted → frameshift`
25.6	`GGCGGC-------------------------------------------------------GCGCCCGGCTATTCTCGCA`	`55 bp deleted → frameshift`
23.2	`GGCGGCCTAAGG---------------------------------------------------------CTATTCTCGCA`	`57 bp deleted → no frameshift`
23.2	`GGCGGC---------------------------------------------------------GCCCGGCTATTCTCGCA`	`57 bp deleted → no frameshift`
21.0	`GGC-------------------------------------------------------------------TATTCTCGCA`	`67 bp deleted → frameshift`
20.0	`GGCGGCCTAAGGACTCGGC------------------------------------------------------------A`	`60 bp deleted → no frameshift`
20.0	`GGCGGCC------------------------------------------------------------GGCTATTCTCGCA`	`60 bp deleted → no frameshift`
18.3	`GGCGGCCTAAGGACT--------------------------------------------------------ATTCTCGCA`	`56 bp deleted → frameshift`
18.0	`GGC--------------------------------------------------------------CCGGCTATTCTCGCA`	`62 bp deleted → frameshift`
17.2	`GGCGGCCTA---------------------------------------------------------------TTCTCGCA`	`63 bp deleted → no frameshift`
15.6	`GGCGGCCTAAGGACTC-----------------------------------------------------------TCGCA`	`59 bp deleted → frameshift`
10.4	`GGCGGC-------------------------------------------------------------------------A`	`73 bp deleted → frameshift`
10.0	`GGCG--------------------------------------------------------------------------CA`	`74 bp deleted → frameshift`
9.9	`GGCGGCCT--------------------------------------------------------------------CGCA`	`68 bp deleted → frameshift`
8.8	`GGC----------------------------------------------------------------------------A`	`76 bp deleted → frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License