CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCCGAGTCCTTAGGCCGCC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	(Wild-type sequence)
485.6	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC----------GGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`10 bp deleted → frameshift`
417.6	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCG-------------GCGCGCGCCCAGATTGGGGACAAAGGAAG`	`13 bp deleted → frameshift`
346.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`11 bp deleted → frameshift`
335.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG--------GGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`8 bp deleted → frameshift`
329.4	`CGCCCTGGCCACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGACAAAGGAAG`	`34 bp deleted → frameshift`
327.6	`CGCCCTGGCCACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAG`	`26 bp deleted → frameshift`
294.4	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC--------------------CAGATTGGGGACAAAGGAAG`	`20 bp deleted → frameshift`
269.4	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC----------------GCGCCCAGATTGGGGACAAAGGAAG`	`16 bp deleted → frameshift`
268.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`8 bp deleted → frameshift`
256.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------------GCGCGCCCAGATTGGGGACAAAGGAAG`	`17 bp deleted → frameshift`
255.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`9 bp deleted → no frameshift`
247.0	`CGCCCTGGCCACTTCCGGCGC----------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`28 bp deleted → frameshift`
244.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC------------------GCCCAGATTGGGGACAAAGGAAG`	`18 bp deleted → no frameshift`
242.8	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`10 bp deleted → frameshift`
231.0	`CGCCCTGGCCACTTCCGGCGCGCC------------------------------------CAGATTGGGGACAAAGGAAG`	`36 bp deleted → no frameshift`
198.8	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------------GCGCGCCCAGATTGGGGACAAAGGAAG`	`14 bp deleted → frameshift`
190.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC-----------------------CAGATTGGGGACAAAGGAAG`	`23 bp deleted → frameshift`
161.6	`CGCCCTGGCCACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGACAAAGGAAG`	`32 bp deleted → frameshift`
154.8	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-------------------GCGCCCAGATTGGGGACAAAGGAAG`	`19 bp deleted → frameshift`
147.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCC--------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`20 bp deleted → frameshift`
141.6	`CGCCCTGGCCACTTCCGGCGCGCCGAG---------------GGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`15 bp deleted → no frameshift`
140.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC---------------------AGATTGGGGACAAAGGAAG`	`21 bp deleted → no frameshift`
140.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC---------------------GCCCAGATTGGGGACAAAGGAAG`	`21 bp deleted → no frameshift`
126.8	`CGCCCTGGCCACTTCCGGCGCGCCG-----------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`23 bp deleted → frameshift`
120.4	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC------------------------AGATTGGGGACAAAGGAAG`	`24 bp deleted → no frameshift`
120.4	`CGCCCTGGCCACTTCCGGCGCGC------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`24 bp deleted → no frameshift`
120.0	`CGCCCTGGCCACTTCCGGCGC--------------------------------------CCAGATTGGGGACAAAGGAAG`	`38 bp deleted → frameshift`
114.8	`CGCCCTGGCCACTTCCGG-------------------------GGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`25 bp deleted → frameshift`
109.2	`CGCCCTGGCCACTTCCGG--------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`26 bp deleted → frameshift`
103.6	`CGCCCTGGCCACTTCCGG---------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`27 bp deleted → no frameshift`
94.0	`CGCCCTGGCCACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGACAAAGGAAG`	`29 bp deleted → frameshift`
94.0	`CGCCCTGGCCACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAG`	`29 bp deleted → frameshift`
90.0	`CGCCCTGGC--------------------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`38 bp deleted → frameshift`
89.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGACAAAGGAAG`	`30 bp deleted → no frameshift`
89.2	`CGCCCTGGCCACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGACAAAGGAAG`	`30 bp deleted → no frameshift`
89.2	`CGCCCTGGCCACTTCCGGC------------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`30 bp deleted → no frameshift`
84.8	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGACAAAGGAAG`	`31 bp deleted → frameshift`
84.8	`CGCCCTGGCCACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGACAAAGGAAG`	`31 bp deleted → frameshift`
84.8	`CGCCCTGGCCACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAG`	`31 bp deleted → frameshift`
84.8	`CGCCCTGGCCACTTCCG-------------------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`31 bp deleted → frameshift`
81.0	`CGCCCTGGCC----------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`40 bp deleted → frameshift`
80.0	`CGCC----------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`46 bp deleted → frameshift`
76.8	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------------------------------GGACAAAGGAAG`	`33 bp deleted → no frameshift`
76.8	`CGCCCTGGCCACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGACAAAGGAAG`	`33 bp deleted → no frameshift`
73.2	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------------------------------GACAAAGGAAG`	`34 bp deleted → frameshift`
70.5	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG-----------------------------ATTGGGGACAAAGGAAG`	`29 bp deleted → frameshift`
69.6	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG-----------------------------------ACAAAGGAAG`	`35 bp deleted → frameshift`
69.6	`CGCCCTGG-----------------------------------GGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`35 bp deleted → frameshift`
66.6	`CGCCCTGGC--------------------------------------------GCGCGCCCAGATTGGGGACAAAGGAAG`	`44 bp deleted → frameshift`
66.0	`CGCCCTGG------------------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`36 bp deleted → no frameshift`
62.8	`CGCCCTGGCCACTTCCGGCGCGCC-------------------------------------AGATTGGGGACAAAGGAAG`	`37 bp deleted → frameshift`
62.8	`CGCCCTGGCCACTTCCG-------------------------------------CGCGCCCAGATTGGGGACAAAGGAAG`	`37 bp deleted → frameshift`
62.8	`CGCCCTGG-------------------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`37 bp deleted → frameshift`
61.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------AAG`	`42 bp deleted → no frameshift`
61.0	`CGCCC--------------------------------------------------------AGATTGGGGACAAAGGAAG`	`56 bp deleted → frameshift`
56.8	`CGCCCTGGCCACTTCCG---------------------------------------CGCCCAGATTGGGGACAAAGGAAG`	`39 bp deleted → no frameshift`
54.0	`CGCCCTGGCCACTTCCGGC----------------------------------------CCAGATTGGGGACAAAGGAAG`	`40 bp deleted → frameshift`
51.6	`CGCCCTGGCCACTTCCG-----------------------------------------CCCAGATTGGGGACAAAGGAAG`	`41 bp deleted → frameshift`
49.5	`CGCCCTGGCCACTTCCGGCGCGCCGAG------------------------------------ATTGGGGACAAAGGAAG`	`36 bp deleted → no frameshift`
49.2	`CGCCCTGGCC--------------------------------------------------CAGATTGGGGACAAAGGAAG`	`50 bp deleted → frameshift`
45.0	`CGCCCTGGCCACTTCCGGCGCGCCGA--------------------------------------TTGGGGACAAAGGAAG`	`38 bp deleted → frameshift`
44.4	`CGCCCTGGCCACTTCC--------------------------------------------CAGATTGGGGACAAAGGAAG`	`44 bp deleted → frameshift`
44.4	`CGC--------------------------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`44 bp deleted → frameshift`
44.4	`CGC----------------------------------------------------GCGCCCAGATTGGGGACAAAGGAAG`	`52 bp deleted → frameshift`
42.0	`CGCCCTGGCCACTTCC---------------------------------------------AGATTGGGGACAAAGGAAG`	`45 bp deleted → no frameshift`
42.0	`CGCCC---------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAG`	`45 bp deleted → no frameshift`
40.2	`CGC------------------------------------------------------GCCCAGATTGGGGACAAAGGAAG`	`54 bp deleted → no frameshift`
40.0	`CGCCCTGGC----------------------------------------------GCGCCCAGATTGGGGACAAAGGAAG`	`46 bp deleted → frameshift`
39.0	`CGCCCTGGCCA---------------------------------------------------GATTGGGGACAAAGGAAG`	`51 bp deleted → no frameshift`
36.6	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGACAAAGGAAG`	`34 bp deleted → frameshift`
36.4	`CGCCCTGGC------------------------------------------------GCCCAGATTGGGGACAAAGGAAG`	`48 bp deleted → no frameshift`
34.4	`CG-------------------------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAG`	`49 bp deleted → frameshift`
32.8	`CGCCCTGGCCACTTCCGG--------------------------------------------------GGACAAAGGAAG`	`50 bp deleted → frameshift`
32.8	`CGC--------------------------------------------------GCGCGCCCAGATTGGGGACAAAGGAAG`	`50 bp deleted → frameshift`
31.5	`CGCCCTGGCCACTTCCGGCGCGCCGA---------------------------------------------CAAAGGAAG`	`45 bp deleted → no frameshift`
31.2	`CGCCCTGGCCACTTCCGG---------------------------------------------------GACAAAGGAAG`	`51 bp deleted → no frameshift`
30.0	`CGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG----------------------------------------------`	`46 bp deleted → frameshift`
29.6	`CGCCCTGGCCACTTCCGG----------------------------------------------------ACAAAGGAAG`	`52 bp deleted → frameshift`
25.8	`CGCCCTGGCCACTTCCGGCGCGCCGAG-------------------------------------------------GAAG`	`49 bp deleted → frameshift`
25.6	`CGCCC-------------------------------------------------------CAGATTGGGGACAAAGGAAG`	`55 bp deleted → frameshift`
25.0	`CGCCCTGG------------------------------------------------------------GGACAAAGGAAG`	`60 bp deleted → no frameshift`
23.2	`CGCC---------------------------------------------------------AGATTGGGGACAAAGGAAG`	`57 bp deleted → no frameshift`
22.2	`CGCCCTGGCCACTTCCGGCGCGCCGA----------------------------------------------------AG`	`52 bp deleted → frameshift`
21.3	`CGCCCTGGCCACTTCCGGCGCGCCGAG-----------------------------------------------------`	`53 bp deleted → frameshift`
20.8	`CGCCCTGGCCACTTCCGG-----------------------------------------------------------AAG`	`59 bp deleted → frameshift`
18.8	`CGCCCTGG-------------------------------------------------------------GACAAAGGAAG`	`61 bp deleted → frameshift`
18.0	`CGCCCTGG--------------------------------------------------------------ACAAAGGAAG`	`62 bp deleted → frameshift`
15.0	`CGCCCTGGCCAC------------------------------------------------------------AAAGGAAG`	`60 bp deleted → no frameshift`
14.8	`CGCCCTGGCCACTT----------------------------------------------------GGGGACAAAGGAAG`	`52 bp deleted → frameshift`
13.5	`CGCCCTGGCCA--------------------------------------------------------------AAGGAAG`	`62 bp deleted → frameshift`
12.8	`CGCCCTGG---------------------------------------------------------------------AAG`	`69 bp deleted → no frameshift`

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