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The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| Bae-Score | Sequence | Effect |
|---|---|---|
| GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | (Wild-type sequence) | |
| 327.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC----CTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 4 bp deleted → frameshift |
| 313.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCC-------------TTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 13 bp deleted → frameshift |
| 296.4 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCG------GCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 6 bp deleted → no frameshift |
| 268.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG--------GCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 8 bp deleted → frameshift |
| 255.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC---------ACATGCCGGAGCCGTTGTCGACGACGAGCGC | 9 bp deleted → no frameshift |
| 255.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGC---------CTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 9 bp deleted → no frameshift |
| 223.0 | GAGGGGAAGACGGCC------------------------------TTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 30 bp deleted → no frameshift |
| 219.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCC------------TTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 12 bp deleted → no frameshift |
| 219.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG------------GCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 12 bp deleted → no frameshift |
| 210.0 | GAGGGGAAGACGGCCCGGGGGGC---------------------CTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 21 bp deleted → no frameshift |
| 198.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGC--------------ACATGCCGGAGCCGTTGTCGACGACGAGCGC | 14 bp deleted → frameshift |
| 190.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG-----------------------GAGCCGTTGTCGACGACGAGCGC | 23 bp deleted → frameshift |
| 188.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC---------------CGGAGCCGTTGTCGACGACGAGCGC | 15 bp deleted → no frameshift |
| 188.8 | GAGGGGAAGACGGCCCGGGGGGCATCG---------------GCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 15 bp deleted → no frameshift |
| 180.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCC------------------------GGAGCCGTTGTCGACGACGAGCGC | 24 bp deleted → no frameshift |
| 175.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC---------------------CGTTGTCGACGACGAGCGC | 21 bp deleted → no frameshift |
| 172.2 | GAGGGGAAGACGGCCCGG-------------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 25 bp deleted → frameshift |
| 162.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGC------------------ACATGCCGGAGCCGTTGTCGACGACGAGCGC | 18 bp deleted → no frameshift |
| 147.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGC--------------------CGGAGCCGTTGTCGACGACGAGCGC | 20 bp deleted → frameshift |
| 141.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG-----------------------------TTGTCGACGACGAGCGC | 29 bp deleted → frameshift |
| 140.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCG---------------------GAGCCGTTGTCGACGACGAGCGC | 21 bp deleted → no frameshift |
| 136.5 | GAGGGGAAGACGGCCCGGGGGGCA--------------------------CATGCCGGAGCCGTTGTCGACGACGAGCGC | 26 bp deleted → frameshift |
| 133.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCC------------------------------GTTGTCGACGACGAGCGC | 30 bp deleted → no frameshift |
| 133.2 | GAGGGGAAGACGGCCCGGGGG----------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 22 bp deleted → frameshift |
| 126.8 | GAGGGGAAGACGGCCCGGGG-----------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 23 bp deleted → frameshift |
| 120.4 | GAGGGGAAGACGGCCCGGG------------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 24 bp deleted → no frameshift |
| 109.2 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGC--------------------------CGTTGTCGACGACGAGCGC | 26 bp deleted → frameshift |
| 108.0 | GAGGGGAAGACGGCCCGG----------------------------------------AGCCGTTGTCGACGACGAGCGC | 40 bp deleted → frameshift |
| 103.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCG---------------------------TTGTCGACGACGAGCGC | 27 bp deleted → no frameshift |
| 103.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG---------------------------GAGCCGTTGTCGACGACGAGCGC | 27 bp deleted → no frameshift |
| 99.9 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGA----------------------GCCGTTGTCGACGACGAGCGC | 22 bp deleted → frameshift |
| 99.4 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG---------------------------------------ACGACGAGCGC | 39 bp deleted → no frameshift |
| 98.8 | GAGGGGAAGACGGCCCGGGGGGCAT----------------------------GCCGGAGCCGTTGTCGACGACGAGCGC | 28 bp deleted → frameshift |
| 96.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGA---------------------------------CGACGAGCGC | 33 bp deleted → no frameshift |
| 94.0 | GAGGGGAAGACGGCCC-----------------------------TTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 29 bp deleted → frameshift |
| 89.2 | GAGGGGAAGACGGCCCGGGGGGCATCG------------------------------GAGCCGTTGTCGACGACGAGCGC | 30 bp deleted → no frameshift |
| 82.5 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGA------------------------------------CGAGCGC | 36 bp deleted → no frameshift |
| 82.5 | GAGGGGAAGACGGCCCGGGGGGCATCGT------------------------------------TGTCGACGACGAGCGC | 36 bp deleted → no frameshift |
| 80.8 | GAGGGGAAGACGGCCCGGGGGGC--------------------------------CGGAGCCGTTGTCGACGACGAGCGC | 32 bp deleted → frameshift |
| 77.4 | GAGGGGAAGACGGCC-----------------------------------------GGAGCCGTTGTCGACGACGAGCGC | 41 bp deleted → frameshift |
| 76.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG---------------------------------TTGTCGACGACGAGCGC | 33 bp deleted → no frameshift |
| 75.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC--------------------------------------GC | 38 bp deleted → frameshift |
| 71.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGA---------------------------------------GCGC | 39 bp deleted → no frameshift |
| 69.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCG-------------------------------------------C | 43 bp deleted → frameshift |
| 69.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG-----------------------------------ACGACGAGCGC | 35 bp deleted → frameshift |
| 69.6 | GAGGGGAAGACGGC-----------------------------------ACATGCCGGAGCCGTTGTCGACGACGAGCGC | 35 bp deleted → frameshift |
| 66.0 | GAGGGGAAGACGGCCCGGGGGG------------------------------------AGCCGTTGTCGACGACGAGCGC | 36 bp deleted → no frameshift |
| 63.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGC--------------------------------------------- | 45 bp deleted → no frameshift |
| 63.0 | GAGGGGAAGACGG---------------------------------------------AGCCGTTGTCGACGACGAGCGC | 45 bp deleted → no frameshift |
| 62.8 | GAGGGGAAGACGGCCCGGGGG-------------------------------------AGCCGTTGTCGACGACGAGCGC | 37 bp deleted → frameshift |
| 62.8 | GAGGGG-------------------------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 37 bp deleted → frameshift |
| 61.0 | GAGGGGAAGACGGCCCGGGGGGCATCG------------------------------------------ACGACGAGCGC | 42 bp deleted → no frameshift |
| 60.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG--------------------------------------ACGAGCGC | 38 bp deleted → frameshift |
| 60.0 | GAGGGGAAGACGGCCCGGGGGGC--------------------------------------CGTTGTCGACGACGAGCGC | 38 bp deleted → frameshift |
| 60.0 | GAGGGGAAGACGGCCCGGGG--------------------------------------AGCCGTTGTCGACGACGAGCGC | 38 bp deleted → frameshift |
| 60.0 | GAGGGGAAGACGGCCCG----------------------------------------------TTGTCGACGACGAGCGC | 46 bp deleted → frameshift |
| 60.0 | GAGGG--------------------------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 38 bp deleted → frameshift |
| 57.0 | GAGGGGAAGACGGCC-----------------------------------------------GTTGTCGACGACGAGCGC | 47 bp deleted → frameshift |
| 56.8 | GAGGGGAAGACGGCCCGGG---------------------------------------AGCCGTTGTCGACGACGAGCGC | 39 bp deleted → no frameshift |
| 56.8 | GAGG---------------------------------------CCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGC | 39 bp deleted → no frameshift |
| 54.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGC---------------------------------------- | 40 bp deleted → frameshift |
| 51.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCG-----------------------------------------AGCGC | 41 bp deleted → frameshift |
| 51.6 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGC------------------------------------------------- | 49 bp deleted → frameshift |
| 48.8 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG------------------------------------------ACGAGCGC | 42 bp deleted → no frameshift |
| 42.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCG---------------------------------------------AGCGC | 45 bp deleted → no frameshift |
| 42.0 | GAGGGGAAGACGGCCCGGGGGGCATCG---------------------------------------------ACGAGCGC | 45 bp deleted → no frameshift |
| 40.5 | GAGGGGAAGAC----------------------------------------ATGCCGGAGCCGTTGTCGACGACGAGCGC | 40 bp deleted → frameshift |
| 38.0 | GAGGGGAAGACGGCCCGGGGGGCATCGTCGC-----------------------------------------------GC | 47 bp deleted → frameshift |
| 37.0 | GAGGGGA----------------------------------------------------GCCGTTGTCGACGACGAGCGC | 52 bp deleted → frameshift |
| 36.4 | GAGGGGAAGACGGCCCGGGGGGCATCG------------------------------------------------AGCGC | 48 bp deleted → no frameshift |
| 35.0 | GAGGGGAAGACG------------------------------------------------------------ACGAGCGC | 60 bp deleted → no frameshift |
| 31.2 | GAGGGGAAGACG---------------------------------------------------TTGTCGACGACGAGCGC | 51 bp deleted → no frameshift |
| 30.1 | GAGGGGAAGACG---------------------------------------------------------------AGCGC | 63 bp deleted → no frameshift |
| 29.6 | GAGGGGAAGACGGCCCGGGGGGCATCG----------------------------------------------------C | 52 bp deleted → frameshift |
| 29.6 | GAGGGGAAGACGGCCCG----------------------------------------------------ACGACGAGCGC | 52 bp deleted → frameshift |
| 29.0 | GAG---------------------------------------------------------CCGTTGTCGACGACGAGCGC | 57 bp deleted → no frameshift |
| 28.4 | GAGGG-----------------------------------------------------AGCCGTTGTCGACGACGAGCGC | 53 bp deleted → frameshift |
| 26.8 | GAGG------------------------------------------------------AGCCGTTGTCGACGACGAGCGC | 54 bp deleted → no frameshift |
| 25.8 | GAGGGGAAGA-------------------------------------------------GCCGTTGTCGACGACGAGCGC | 49 bp deleted → frameshift |
| 25.6 | GAGGGGAAGACGGCCCGGGGGGC-------------------------------------------------------GC | 55 bp deleted → frameshift |
| 25.6 | GAGGGGAAGACGGCCCG-------------------------------------------------------ACGAGCGC | 55 bp deleted → frameshift |
| 23.4 | GAGGGGAAG---------------------------------------------------CCGTTGTCGACGACGAGCGC | 51 bp deleted → no frameshift |
| 23.2 | GAGGGGAAGACGGCCCGGGGGGC--------------------------------------------------------- | 57 bp deleted → no frameshift |
| 23.2 | GAGGGGAAGACG---------------------------------------------------------ACGACGAGCGC | 57 bp deleted → no frameshift |
| 22.0 | GAGGGGAAGACGGCCCG----------------------------------------------------------AGCGC | 58 bp deleted → frameshift |
| 18.0 | GAGGGGAAGACGGCCCG--------------------------------------------------------------C | 62 bp deleted → frameshift |
| 16.4 | GAGGGGAAGACGGC----------------------------------------------------------------GC | 64 bp deleted → frameshift |
| 14.8 | GAGGGGAAGACGGC------------------------------------------------------------------ | 66 bp deleted → no frameshift |
| 14.0 | GAGGGGAAGACG-------------------------------------------------------------------C | 67 bp deleted → frameshift |
| 12.9 | GAGGGGA---------------------------------------------------------------CGACGAGCGC | 63 bp deleted → no frameshift |
| 12.5 | GAG--------------------------------------------------------------------------CGC | 74 bp deleted → frameshift |
| 11.1 | GAGGGGAAGA------------------------------------------------------------------GCGC | 66 bp deleted → no frameshift |
| 11.1 | GAGGGGA------------------------------------------------------------------CGAGCGC | 66 bp deleted → no frameshift |
| 9.9 | GAGGGGAAG--------------------------------------------------------------------CGC | 68 bp deleted → frameshift |
| 9.9 | GA--------------------------------------------------------------------CGACGAGCGC | 68 bp deleted → frameshift |
| 9.6 | GAGGGGA---------------------------------------------------------------------GCGC | 69 bp deleted → no frameshift |
| 8.7 | GA-----------------------------------------------------------------------CGAGCGC | 71 bp deleted → frameshift |