CRISPOR

Micro-homology scoring of potential deletions

Guide: ATGGAGGGGAAGACGGCCCG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	(Wild-type sequence)
427.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-----------------CGAAGCCGGCCTTGCACATGCCG`	`17 bp deleted → frameshift`
382.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`9 bp deleted → no frameshift`
377.6	`GGTGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`15 bp deleted → no frameshift`
359.2	`GGTGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`16 bp deleted → frameshift`
298.2	`GGTGCCTGGGGCGCCCCACGATGGAGGGG--------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`14 bp deleted → frameshift`
296.4	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`6 bp deleted → no frameshift`
282.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`7 bp deleted → frameshift`
268.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG--------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`8 bp deleted → frameshift`
256.2	`GGTGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`17 bp deleted → frameshift`
242.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG----------TCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`10 bp deleted → frameshift`
223.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------------------TTGCACATGCCG`	`30 bp deleted → no frameshift`
208.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------CCCGCGAAGCCGGCCTTGCACATGCCG`	`13 bp deleted → frameshift`
208.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGG-------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`13 bp deleted → frameshift`
188.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------TCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`15 bp deleted → no frameshift`
183.0	`GGTGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`34 bp deleted → frameshift`
179.6	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC----------------CGCGAAGCCGGCCTTGCACATGCCG`	`16 bp deleted → frameshift`
172.2	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------------------GCCTTGCACATGCCG`	`25 bp deleted → frameshift`
163.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC--------------------------GGCCTTGCACATGCCG`	`26 bp deleted → frameshift`
162.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------GCGAAGCCGGCCTTGCACATGCCG`	`18 bp deleted → no frameshift`
162.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------CCCGCGAAGCCGGCCTTGCACATGCCG`	`18 bp deleted → no frameshift`
162.8	`GGTGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`18 bp deleted → no frameshift`
162.8	`GGTGCCTGGGGCGCCCCACGATGG------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`18 bp deleted → no frameshift`
154.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------------AAGCCGGCCTTGCACATGCCG`	`19 bp deleted → frameshift`
154.8	`GGTGCCTGGGGCGCCCCACGATGG-------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`19 bp deleted → frameshift`
153.6	`GGTGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`33 bp deleted → no frameshift`
147.2	`GGTGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`20 bp deleted → frameshift`
140.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------------------GAAGCCGGCCTTGCACATGCCG`	`21 bp deleted → no frameshift`
140.0	`GGTGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`21 bp deleted → no frameshift`
135.0	`GGTGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCACATGCCG`	`40 bp deleted → frameshift`
133.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCACATGCCG`	`30 bp deleted → no frameshift`
133.2	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG----------------------CGAAGCCGGCCTTGCACATGCCG`	`22 bp deleted → frameshift`
121.2	`GGTGCCTGGGG--------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`32 bp deleted → frameshift`
120.4	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------------AAGCCGGCCTTGCACATGCCG`	`24 bp deleted → no frameshift`
104.4	`GGTGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`35 bp deleted → frameshift`
94.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-----------------------------TTGCACATGCCG`	`29 bp deleted → frameshift`
89.2	`GGTGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`30 bp deleted → no frameshift`
85.2	`GGTGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCACATGCCG`	`39 bp deleted → no frameshift`
84.8	`GGTGCCTGGGG-------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`31 bp deleted → frameshift`
81.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG----------------------------------------`	`40 bp deleted → frameshift`
80.8	`GGTGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`32 bp deleted → frameshift`
77.7	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCACATGCCG`	`27 bp deleted → no frameshift`
77.4	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC-----------------------------------------G`	`41 bp deleted → frameshift`
76.8	`GGTGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCACATGCCG`	`33 bp deleted → no frameshift`
71.0	`GGTGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCACATGCCG`	`39 bp deleted → no frameshift`
70.5	`GGTGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`29 bp deleted → frameshift`
69.6	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------ACATGCCG`	`35 bp deleted → frameshift`
66.6	`GGTGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCACATGCCG`	`44 bp deleted → frameshift`
66.0	`GGTGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`36 bp deleted → no frameshift`
62.8	`GGTGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCACATGCCG`	`37 bp deleted → frameshift`
62.8	`GGTGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCACATGCCG`	`37 bp deleted → frameshift`
62.8	`GGTGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`37 bp deleted → frameshift`
60.0	`GGTGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCACATGCCG`	`38 bp deleted → frameshift`
60.0	`GGTGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCACATGCCG`	`38 bp deleted → frameshift`
60.0	`GGTGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCACATGCCG`	`46 bp deleted → frameshift`
56.8	`GGTGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCACATGCCG`	`39 bp deleted → no frameshift`
54.6	`GGTGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`26 bp deleted → frameshift`
54.0	`GG----------------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`40 bp deleted → frameshift`
52.2	`GGTGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCACATGCCG`	`35 bp deleted → frameshift`
51.6	`GGTGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCACATGCCG`	`41 bp deleted → frameshift`
51.6	`GGTGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`41 bp deleted → frameshift`
51.6	`GG-----------------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`41 bp deleted → frameshift`
51.6	`GGTGCCTGGGGCGCC-------------------------------------------------GGCCTTGCACATGCCG`	`49 bp deleted → frameshift`
51.6	`GGTGCC-------------------------------------------------CGCGAAGCCGGCCTTGCACATGCCG`	`49 bp deleted → frameshift`
49.5	`GGTGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCACATGCCG`	`36 bp deleted → no frameshift`
48.8	`GGTGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCACATGCCG`	`42 bp deleted → no frameshift`
48.8	`GGTGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCACATGCCG`	`42 bp deleted → no frameshift`
48.8	`GG------------------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`42 bp deleted → no frameshift`
46.4	`GG-------------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCG`	`43 bp deleted → frameshift`
42.6	`GGTGCCTGGGGCGCC-----------------------------------------------------TTGCACATGCCG`	`53 bp deleted → frameshift`
42.0	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------------------------------------`	`45 bp deleted → no frameshift`
42.0	`GGTGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCACATGCCG`	`45 bp deleted → no frameshift`
40.5	`GGTGCCTGGGGCGCCCCACGATGGAGGGGAAGAC----------------------------------------ATGCCG`	`40 bp deleted → frameshift`
38.4	`GGTGCCTGGGGC-------------------------------------------------------CTTGCACATGCCG`	`55 bp deleted → frameshift`
38.0	`GGTGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCACATGCCG`	`47 bp deleted → frameshift`
36.4	`GGTGCCTGGGGCGCCC------------------------------------------------GGCCTTGCACATGCCG`	`48 bp deleted → no frameshift`
33.0	`GGTGCC----------------------------------------------------------GGCCTTGCACATGCCG`	`58 bp deleted → frameshift`
32.8	`GGTGCC--------------------------------------------------GCGAAGCCGGCCTTGCACATGCCG`	`50 bp deleted → frameshift`
32.0	`GGTGCCTGGGGCGCCCCAC-------------------------------------------------------ATGCCG`	`55 bp deleted → frameshift`
31.5	`GGTGCCT--------------------------------------------------------------TGCACATGCCG`	`62 bp deleted → frameshift`
31.2	`GGTGCCTGGGGCGCCCC---------------------------------------------------TTGCACATGCCG`	`51 bp deleted → no frameshift`
31.2	`GGTGCCTGGGGC---------------------------------------------------CGGCCTTGCACATGCCG`	`51 bp deleted → no frameshift`
29.6	`GGTGCCTGGGGCGCCC----------------------------------------------------TTGCACATGCCG`	`52 bp deleted → frameshift`
29.6	`GGTGCCTGGGGCG----------------------------------------------------GCCTTGCACATGCCG`	`52 bp deleted → frameshift`
28.4	`GGTGC-----------------------------------------------------GAAGCCGGCCTTGCACATGCCG`	`53 bp deleted → frameshift`
27.3	`GGTGCCTGGGGCGCCCCACGATG------------------------------------------------CACATGCCG`	`48 bp deleted → no frameshift`
27.3	`GGT------------------------------------------------CGCCCGCGAAGCCGGCCTTGCACATGCCG`	`48 bp deleted → no frameshift`
26.8	`GGTGCCTGGGGCGCCCCACGATG------------------------------------------------------CCG`	`54 bp deleted → no frameshift`
24.6	`GGTGCCTGGGGCGCC----------------------------------------------------------------G`	`64 bp deleted → frameshift`
24.4	`GGTGCCTGGG--------------------------------------------------------CCTTGCACATGCCG`	`56 bp deleted → frameshift`
23.2	`GGTGCCTGG---------------------------------------------------------CCTTGCACATGCCG`	`57 bp deleted → no frameshift`
22.0	`GGTGCCTGGGGCGC----------------------------------------------------------ACATGCCG`	`58 bp deleted → frameshift`
20.0	`GGTGCCTGGGGCGCCCCACG------------------------------------------------------------`	`60 bp deleted → no frameshift`
20.0	`GGTGCCTGGGGC------------------------------------------------------------ACATGCCG`	`60 bp deleted → no frameshift`
18.2	`GGTGCC-------------------------------------------------------------------------G`	`73 bp deleted → frameshift`
18.0	`GGTGCCTGGGGCGCCCC--------------------------------------------------------------G`	`62 bp deleted → frameshift`
17.5	`GGTGC-------------------------------------------------------------------ACATGCCG`	`67 bp deleted → frameshift`
17.4	`GGTGCCTGGGGCGCCCCA---------------------------------------------------------TGCCG`	`57 bp deleted → no frameshift`
17.2	`GGTGCCTGGGGCGCCC---------------------------------------------------------------G`	`63 bp deleted → no frameshift`
16.4	`GG----------------------------------------------------------------CCTTGCACATGCCG`	`64 bp deleted → frameshift`
14.8	`GGTGCCTGGGGC------------------------------------------------------------------CG`	`66 bp deleted → no frameshift`
14.0	`GGTGCCTGGGGCG-------------------------------------------------------------------`	`67 bp deleted → frameshift`
12.9	`GGTGCCTG---------------------------------------------------------------CACATGCCG`	`63 bp deleted → no frameshift`
9.6	`GGTGCCTG---------------------------------------------------------------------CCG`	`69 bp deleted → no frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License