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Contents:

PCR primers for off-targets of GCGCCGGCGCGCGCCCAGAT TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529749_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529749_R Primer3: not found at this Tm N.d. 1.00
mm4__chr19 9.83 Mbp_chr19_9834992_F TCGTCGGCAGCGTCCATAAGCTCTATCCCGCCTGG 60.0 0.28
mm4__chr19 9.83 Mbp_chr19_9834992_R GTCTCGTGGGCTCGGGCCCTTCTCAACGCCCTTTA 60.3 0.28
mm4__chr22 41.67 Mbp_chr22_41666852_F TCGTCGGCAGCGTCGACTTCTACCGCGGCCATC 60.5 0.16
mm4__chr22 41.67 Mbp_chr22_41666852_R GTCTCGTGGGCTCGGGACTCTCCGGCTTATGGCTC 59.9 0.16
mm4__chr22 44.33 Mbp_chr22_44331164_F TCGTCGGCAGCGTCTTCCTCTCCAAGGTTCAGCG 59.6 0.13
mm4__chr22 44.33 Mbp_chr22_44331164_R GTCTCGTGGGCTCGGCGGGGCTCTTTAGAAAGGGG 60.1 0.13
mm3__chr11 95.15 Mbp_chr11_95150808_F TCGTCGGCAGCGTCCGGCCGGGAGCTATTACAG 60.0 0.11
mm3__chr11 95.15 Mbp_chr11_95150808_R GTCTCGTGGGCTCGGTGAGAGAGGCCAGCAGGTAT 60.0 0.11
mm4__chr2 127.03 Mbp_chr2_127025564_F Primer3: not found at this Tm N.d. 0.09
mm4__chr2 127.03 Mbp_chr2_127025564_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 42.42 Mbp_chr17_42422039_F TCGTCGGCAGCGTCGTGTCCTCAAGGGCCTGAAA 59.8 0.09
mm4__chr17 42.42 Mbp_chr17_42422039_R GTCTCGTGGGCTCGGAACCTCCTCCCCAGACCC 59.8 0.09
mm4__chr12 111.93 Mbp_chr12_111932951_F Primer3: not found at this Tm N.d. 0.09
mm4__chr12 111.93 Mbp_chr12_111932951_R Primer3: not found at this Tm N.d. 0.09
mm3__chr4 1.56 Mbp_chr4_1562486_F TCGTCGGCAGCGTCGAGGCCGGTGTCATTAGTGG 60.4 0.08
mm3__chr4 1.56 Mbp_chr4_1562486_R GTCTCGTGGGCTCGGCTGACCATGTGGAAGGACGT 59.6 0.08
mm4__chr5 157.67 Mbp_chr5_157671537_F TCGTCGGCAGCGTCTATGTGTCTCCCAGGCAACG 59.7 0.07
mm4__chr5 157.67 Mbp_chr5_157671537_R GTCTCGTGGGCTCGGTTGGAAACTGGTGGCTGGTT 60.0 0.07
mm3__chr22 11.83 Mbp_chr22_11825240_F Primer3: not found at this Tm N.d. 0.07
mm3__chr22 11.83 Mbp_chr22_11825240_R Primer3: not found at this Tm N.d. 0.07
mm4__chr7 50.48 Mbp_chr7_50482480_F Primer3: not found at this Tm N.d. 0.07
mm4__chr7 50.48 Mbp_chr7_50482480_R Primer3: not found at this Tm N.d. 0.07
mm4__chr14 60.17 Mbp_chr14_60165310_F TCGTCGGCAGCGTCGTCGCCGTCAGCCCTCAG 62.8 0.07
mm4__chr14 60.17 Mbp_chr14_60165310_R GTCTCGTGGGCTCGGGTGCGGGCCGTCTTCTTC 61.1 0.07
mm3__chr11 68.84 Mbp_chr11_68843977_F TCGTCGGCAGCGTCCCGCTCCAAGGTCATTTCCT 60.0 0.07
mm3__chr11 68.84 Mbp_chr11_68843977_R GTCTCGTGGGCTCGGCCGAAGAGCCCTGGGAAC 59.7 0.07
mm4__chr22 41.29 Mbp_chr22_41286314_F TCGTCGGCAGCGTCCTCGGGTAGAGTAGTCCGGT 59.8 0.06
mm4__chr22 41.29 Mbp_chr22_41286314_R GTCTCGTGGGCTCGGGAAAGAGCCAGAAGCCACCA 60.2 0.06
mm3__chr20 50.15 Mbp_chr20_50153786_F Primer3: not found at this Tm N.d. 0.06
mm3__chr20 50.15 Mbp_chr20_50153786_R Primer3: not found at this Tm N.d. 0.06
mm4__chr8 25.00 Mbp_chr8_25000899_F Primer3: not found at this Tm N.d. 0.06
mm4__chr8 25.00 Mbp_chr8_25000899_R Primer3: not found at this Tm N.d. 0.06
mm3__chr13 27.97 Mbp_chr13_27970495_F TCGTCGGCAGCGTCCTGGAAGTGGCCGTCTTGG 60.6 0.05
mm3__chr13 27.97 Mbp_chr13_27970495_R GTCTCGTGGGCTCGGCTCCACGCGGTTGCTGAA 60.6 0.05
mm3__chr4 1.01 Mbp_chr4_1011271_F TCGTCGGCAGCGTCCGGAGGTGTCTCGAGAGTTG 59.8 0.04
mm3__chr4 1.01 Mbp_chr4_1011271_R GTCTCGTGGGCTCGGCCTAGGACCGGCCAGGAG 60.8 0.04
mm4__chr1 153.89 Mbp_chr1_153893319_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 153.89 Mbp_chr1_153893319_R Primer3: not found at this Tm N.d. 0.04
mm4__chr4 8.16 Mbp_chr4_8159399_F Primer3: not found at this Tm N.d. 0.04
mm4__chr4 8.16 Mbp_chr4_8159399_R Primer3: not found at this Tm N.d. 0.04
mm3__chr17 7.31 Mbp_chr17_7307898_F Primer3: not found at this Tm N.d. 0.04
mm3__chr17 7.31 Mbp_chr17_7307898_R Primer3: not found at this Tm N.d. 0.04
mm4__chr9 91.95 Mbp_chr9_91950673_F TCGTCGGCAGCGTCGAAAACAAACAGGCCGCCTT 59.8 0.03
mm4__chr9 91.95 Mbp_chr9_91950673_R GTCTCGTGGGCTCGGCCCCTGGCAGTTTTGAGCAT 60.9 0.03
mm4__chr18 80.26 Mbp_chr18_80262626_F Primer3: not found at this Tm N.d. 0.03
mm4__chr18 80.26 Mbp_chr18_80262626_R Primer3: not found at this Tm N.d. 0.03
mm3__chr2 45.01 Mbp_chr2_45009481_F TCGTCGGCAGCGTCATTCTAAGCGGGCATGAGGC 60.5 0.03
mm3__chr2 45.01 Mbp_chr2_45009481_R GTCTCGTGGGCTCGGGTGACTGACAGCGTCTCCAT 59.7 0.03
mm4__chr3 4.30 Mbp_chr3_4303508_F TCGTCGGCAGCGTCGTGCGGCCAGGAAAACTTG 60.0 0.03
mm4__chr3 4.30 Mbp_chr3_4303508_R GTCTCGTGGGCTCGGCTTGAGGAGACCACGTGGG 59.7 0.03
mm4__chr14 69.48 Mbp_chr14_69484560_F TCGTCGGCAGCGTCCCTTCTGGACGCACGGAG 60.1 0.03
mm4__chr14 69.48 Mbp_chr14_69484560_R GTCTCGTGGGCTCGGAATTCTGGTGGCCCCGATTG 60.6 0.03
mm3__chr16 229.48 Kbp_chr16_229483_F TCGTCGGCAGCGTCCCACTCGGCTCTTTCCCG 60.1 0.02
mm3__chr16 229.48 Kbp_chr16_229483_R GTCTCGTGGGCTCGGGTTGGACAACTTGCGACCAC 59.9 0.02
mm3__chr17 50.35 Mbp_chr17_50346392_F Primer3: not found at this Tm N.d. 0.02
mm3__chr17 50.35 Mbp_chr17_50346392_R Primer3: not found at this Tm N.d. 0.02
mm4__chr16 400.82 Kbp_chr16_400824_F TCGTCGGCAGCGTCGATGTATGAGCCAGGCAGGG 60.2 0.02
mm4__chr16 400.82 Kbp_chr16_400824_R GTCTCGTGGGCTCGGTTCTCCTTTGGCGGGATCAC 60.0 0.02
mm4__chr17 64.13 Mbp_chr17_64129866_F TCGTCGGCAGCGTCGGCTCACGGGGCATCTGG 62.8 0.02
mm4__chr17 64.13 Mbp_chr17_64129866_R GTCTCGTGGGCTCGGGTGGGGTGGAGGACAGCT 60.9 0.02
mm4__chr9 114.40 Mbp_chr9_114398497_F TCGTCGGCAGCGTCTGGTTTCCTGGAGTTGAGCG 60.2 0.02
mm4__chr9 114.40 Mbp_chr9_114398497_R GTCTCGTGGGCTCGGGGGCCTCAGGGATTATGCAA 59.8 0.02
mm3__chr2 85.54 Mbp_chr2_85539107_F Primer3: not found at this Tm N.d. 0.02
mm3__chr2 85.54 Mbp_chr2_85539107_R Primer3: not found at this Tm N.d. 0.02
mm4__chr19 1.59 Mbp_chr19_1592776_F Primer3: not found at this Tm N.d. 0.02
mm4__chr19 1.59 Mbp_chr19_1592776_R Primer3: not found at this Tm N.d. 0.02
mm4__chr8 140.51 Mbp_chr8_140511287_F Primer3: not found at this Tm N.d. 0.02
mm4__chr8 140.51 Mbp_chr8_140511287_R Primer3: not found at this Tm N.d. 0.02
mm4__chr7 815.84 Kbp_chr7_815836_F Primer3: not found at this Tm N.d. 0.02
mm4__chr7 815.84 Kbp_chr7_815836_R Primer3: not found at this Tm N.d. 0.02
mm4__chr12 120.45 Mbp_chr12_120446514_F TCGTCGGCAGCGTCGGGGCCAAGGAAGGAAGAAA 59.8 0.01
mm4__chr12 120.45 Mbp_chr12_120446514_R GTCTCGTGGGCTCGGCCTTTACCTGAGGATCCGCC 59.8 0.01
mm4__chr14 103.06 Mbp_chr14_103057407_F TCGTCGGCAGCGTCCCATGGGCCGCTCTCCTC 62.2 0.01
mm4__chr14 103.06 Mbp_chr14_103057407_R GTCTCGTGGGCTCGGTCCTCGCCGCTCCTAGTC 60.5 0.01
mm4__chr20 64.29 Mbp_chr20_64286503_F Primer3: not found at this Tm N.d. 0.01
mm4__chr20 64.29 Mbp_chr20_64286503_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 106.59 Mbp_chr12_106586235_F TCGTCGGCAGCGTCCAGTCGACGCACCTTCTGG 60.7 0.01
mm4__chr12 106.59 Mbp_chr12_106586235_R GTCTCGTGGGCTCGGGCTCAGCACCCACTTTTGC 60.0 0.01
mm4__chr3 49.55 Mbp_chr3_49554478_F Primer3: not found at this Tm N.d. 0.01
mm4__chr3 49.55 Mbp_chr3_49554478_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22 46.10 Mbp_chr22_46104463_F TCGTCGGCAGCGTCTTGGAGAGGCCTGGGAGG 60.2 0.01
mm4__chr22 46.10 Mbp_chr22_46104463_R GTCTCGTGGGCTCGGCCAAAGGCTGGCTGGTGC 61.6 0.01
mm4__chr4 159.10 Mbp_chr4_159104137_F Primer3: not found at this Tm N.d. 0.01
mm4__chr4 159.10 Mbp_chr4_159104137_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 223.85 Mbp_chr1_223846065_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 223.85 Mbp_chr1_223846065_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 113.45 Mbp_chr11_113452471_F TCGTCGGCAGCGTCTCCATGTTGAGGAGCAAGGG 59.6 0.01
mm4__chr11 113.45 Mbp_chr11_113452471_R GTCTCGTGGGCTCGGGGGTGGATCTGAATCAGCAGT 59.7 0.01
mm4__chr11 67.27 Mbp_chr11_67266344_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 67.27 Mbp_chr11_67266344_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 55.38 Mbp_chr19_55384373_F TCGTCGGCAGCGTCCAGAGAGAGCGTTCCGGG 59.5 0.00
mm4__chr19 55.38 Mbp_chr19_55384373_R GTCTCGTGGGCTCGGGGAAACCACGACCAGGGTAA 59.6 0.00
mm4__chr17 81.96 Mbp_chr17_81959973_F TCGTCGGCAGCGTCTGTGGTCCGTGGAAAATCCC 60.2 0.00
mm4__chr17 81.96 Mbp_chr17_81959973_R GTCTCGTGGGCTCGGTTCAGTGCGGACGCGTTT 60.5 0.00
mm4__chr12 121.63 Mbp_chr12_121626476_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 121.63 Mbp_chr12_121626476_R Primer3: not found at this Tm N.d. 0.00
mm3__chr17 81.94 Mbp_chr17_81937350_F TCGTCGGCAGCGTCGTACCCACCCCTCAGCGC 62.4 0.00
mm3__chr17 81.94 Mbp_chr17_81937350_R GTCTCGTGGGCTCGGCAGATGATGCAACCGCTCTG 59.3 0.00
mm4__chr1 1.00 Mbp_chr1_1001872_F TCGTCGGCAGCGTCCGAGCACTTAAGCCCCGG 60.5 0.00
mm4__chr1 1.00 Mbp_chr1_1001872_R GTCTCGTGGGCTCGGGACACCATCCACACCACCTC 60.3 0.00
mm4__chr11 111.60 Mbp_chr11_111602407_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 111.60 Mbp_chr11_111602407_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 47.93 Mbp_chr18_47931326_F TCGTCGGCAGCGTCCCTCCCCAGTCTAGCGTGA 60.3 0.00
mm4__chr18 47.93 Mbp_chr18_47931326_R GTCTCGTGGGCTCGGCTCTCCCTCTCCGCCCGT 62.8 0.00
mm4__chrY 57.22 Mbp_chrY_57215860_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215860_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215831_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215831_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215802_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215802_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215773_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215773_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215744_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215744_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215715_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215715_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215686_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215686_R Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215657_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 57.22 Mbp_chrY_57215657_R Primer3: not found at this Tm N.d. 0.00
mm4__chrUn_KI270743v1 167.55 Kbp_chrUn_KI270743v1_167547_F Primer3: not found at this Tm N.d. 0.00
mm4__chrUn_KI270743v1 167.55 Kbp_chrUn_KI270743v1_167547_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 66.27 Mbp_chr9_66268076_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 66.27 Mbp_chr9_66268076_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016948_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016948_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016919_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016919_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016890_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016890_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016861_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016861_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016832_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016832_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016803_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016803_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016774_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016774_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016745_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016745_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016716_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016716_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016687_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016687_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016658_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016658_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016629_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016629_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016600_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016600_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016571_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016571_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016542_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 64.02 Mbp_chr9_64016542_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 63.71 Mbp_chr9_63705754_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 63.71 Mbp_chr9_63705754_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 41.36 Mbp_chr9_41356962_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 41.36 Mbp_chr9_41356962_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 40.11 Mbp_chr9_40108526_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 40.11 Mbp_chr9_40108526_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 11.00 Kbp_chr9_11002_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 11.00 Kbp_chr9_11002_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.91 Kbp_chr5_12913_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.91 Kbp_chr5_12913_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.89 Kbp_chr5_12889_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.89 Kbp_chr5_12889_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.87 Kbp_chr5_12865_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.87 Kbp_chr5_12865_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.84 Kbp_chr5_12841_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.84 Kbp_chr5_12841_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.82 Kbp_chr5_12817_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.82 Kbp_chr5_12817_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.79 Kbp_chr5_12788_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.79 Kbp_chr5_12788_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.76 Kbp_chr5_12759_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.76 Kbp_chr5_12759_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.73 Kbp_chr5_12730_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.73 Kbp_chr5_12730_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.70 Kbp_chr5_12701_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.70 Kbp_chr5_12701_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.67 Kbp_chr5_12672_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.67 Kbp_chr5_12672_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.64 Kbp_chr5_12643_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.64 Kbp_chr5_12643_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.61 Kbp_chr5_12614_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.61 Kbp_chr5_12614_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.59 Kbp_chr5_12585_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.59 Kbp_chr5_12585_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.56 Kbp_chr5_12556_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.56 Kbp_chr5_12556_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.53 Kbp_chr5_12527_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.53 Kbp_chr5_12527_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.50 Kbp_chr5_12498_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.50 Kbp_chr5_12498_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.47 Kbp_chr5_12469_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 12.47 Kbp_chr5_12469_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.52 Mbp_chr4_49519754_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.52 Mbp_chr4_49519754_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.52 Mbp_chr4_49519725_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.52 Mbp_chr4_49519725_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.33 Mbp_chr4_49334711_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.33 Mbp_chr4_49334711_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.28 Mbp_chr4_49275526_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 49.28 Mbp_chr4_49275526_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203963_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203963_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203934_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203934_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203905_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203905_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203876_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203876_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203847_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203847_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203818_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203818_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203789_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203789_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203760_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 190.20 Mbp_chr4_190203760_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.70 Kbp_chr3_11704_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.70 Kbp_chr3_11704_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.68 Kbp_chr3_11675_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.68 Kbp_chr3_11675_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.65 Kbp_chr3_11646_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.65 Kbp_chr3_11646_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.62 Kbp_chr3_11617_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.62 Kbp_chr3_11617_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.59 Kbp_chr3_11588_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.59 Kbp_chr3_11588_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.56 Kbp_chr3_11559_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.56 Kbp_chr3_11559_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.53 Kbp_chr3_11530_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.53 Kbp_chr3_11530_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.50 Kbp_chr3_11501_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.50 Kbp_chr3_11501_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.47 Kbp_chr3_11472_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.47 Kbp_chr3_11472_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.44 Kbp_chr3_11443_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.44 Kbp_chr3_11443_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.41 Kbp_chr3_11414_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.41 Kbp_chr3_11414_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.38 Kbp_chr3_11385_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.38 Kbp_chr3_11385_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.36 Kbp_chr3_11356_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.36 Kbp_chr3_11356_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.33 Kbp_chr3_11327_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.33 Kbp_chr3_11327_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.30 Kbp_chr3_11298_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.30 Kbp_chr3_11298_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.27 Kbp_chr3_11269_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.27 Kbp_chr3_11269_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.24 Kbp_chr3_11240_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.24 Kbp_chr3_11240_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.21 Kbp_chr3_11211_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.21 Kbp_chr3_11211_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.18 Kbp_chr3_11182_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.18 Kbp_chr3_11182_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.15 Kbp_chr3_11153_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.15 Kbp_chr3_11153_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.12 Kbp_chr3_11124_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.12 Kbp_chr3_11124_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.10 Kbp_chr3_11095_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.10 Kbp_chr3_11095_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.07 Kbp_chr3_11066_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.07 Kbp_chr3_11066_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.04 Kbp_chr3_11037_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.04 Kbp_chr3_11037_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.01 Kbp_chr3_11008_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 11.01 Kbp_chr3_11008_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 50.81 Mbp_chr22_50807245_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 50.81 Mbp_chr22_50807245_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 50.81 Mbp_chr22_50807216_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 50.81 Mbp_chr22_50807216_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 12.17 Mbp_chr22_12167201_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 12.17 Mbp_chr22_12167201_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.83 Mbp_chr22_11825152_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.83 Mbp_chr22_11825152_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.83 Mbp_chr22_11825123_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.83 Mbp_chr22_11825123_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11707028_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11707028_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11706999_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11706999_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11706970_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 11.71 Mbp_chr22_11706970_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 8.82 Mbp_chr21_8815421_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 8.82 Mbp_chr21_8815421_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699241_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699241_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699212_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699212_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699183_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699183_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699154_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699154_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699125_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699125_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699096_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699096_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699067_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699067_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699038_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699038_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699009_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46699009_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698980_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698980_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698922_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698922_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698893_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698893_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698864_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698864_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698835_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 46.70 Mbp_chr21_46698835_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182756_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182756_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182727_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182727_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182698_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182698_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182669_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182669_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182640_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182640_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182611_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182611_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182582_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182582_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182553_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 242.18 Mbp_chr2_242182553_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1_KI270706v1_random 157.31 Kbp_chr1_KI270706v1_random_157308_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1_KI270706v1_random 157.31 Kbp_chr1_KI270706v1_random_157308_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607200_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607200_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607169_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607169_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607131_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607131_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607100_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607100_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607066_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607066_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607036_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607036_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607006_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58607006_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606976_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606976_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606946_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606946_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606916_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 58.61 Mbp_chr19_58606916_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 10.58 Kbp_chr16_10579_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 10.58 Kbp_chr16_10579_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 101.98 Mbp_chr15_101980221_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 101.98 Mbp_chr15_101980221_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 101.98 Mbp_chr15_101980192_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 101.98 Mbp_chr15_101980192_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 18.58 Mbp_chr14_18583124_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 18.58 Mbp_chr14_18583124_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353399_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353399_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353370_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353370_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353341_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353341_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353312_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353312_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353283_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 114.35 Mbp_chr13_114353283_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.91 Kbp_chr12_10906_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.91 Kbp_chr12_10906_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.88 Kbp_chr12_10877_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.88 Kbp_chr12_10877_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.85 Kbp_chr12_10848_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.85 Kbp_chr12_10848_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.82 Kbp_chr12_10819_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.82 Kbp_chr12_10819_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.79 Kbp_chr12_10790_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.79 Kbp_chr12_10790_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.76 Kbp_chr12_10761_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 10.76 Kbp_chr12_10761_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076355_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076355_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076326_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076326_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076297_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076297_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076268_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076268_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076239_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076239_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076210_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076210_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076181_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 135.08 Mbp_chr11_135076181_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786520_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786520_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786491_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786491_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786462_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 133.79 Mbp_chr10_133786462_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.95 Mbp_chr1_248945006_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.95 Mbp_chr1_248945006_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.94 Mbp_chr1_248944977_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.94 Mbp_chr1_248944977_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.94 Mbp_chr1_248944948_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 248.94 Mbp_chr1_248944948_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.29 Kbp_chr1_181290_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.29 Kbp_chr1_181290_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.20 Kbp_chr1_181203_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.20 Kbp_chr1_181203_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.17 Kbp_chr1_181174_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 181.17 Kbp_chr1_181174_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.75 Kbp_chr1_10752_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.75 Kbp_chr1_10752_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.72 Kbp_chr1_10723_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.72 Kbp_chr1_10723_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.69 Kbp_chr1_10694_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.69 Kbp_chr1_10694_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.66 Kbp_chr1_10665_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.66 Kbp_chr1_10665_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.64 Kbp_chr1_10636_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 10.64 Kbp_chr1_10636_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 75.64 Mbp_chr18_75639051_F TCGTCGGCAGCGTCGGCAGGACTCTCCACTTGTC 60.0 0.00
mm4__chr18 75.64 Mbp_chr18_75639051_R GTCTCGTGGGCTCGGCGATGAGGGCTGAGGTCATC 59.9 0.00
mm4__chr14 22.84 Mbp_chr14_22837361_F TCGTCGGCAGCGTCCATGCGCGAGAGTTTGTTGT 59.7 0.00
mm4__chr14 22.84 Mbp_chr14_22837361_R GTCTCGTGGGCTCGGCAGACTCGTCCGCTGAAGG 60.1 0.00
mm4__chr20 63.83 Mbp_chr20_63831196_F Primer3: not found at this Tm N.d. 0.00
mm4__chr20 63.83 Mbp_chr20_63831196_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 18.18 Mbp_chr17_18183723_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 18.18 Mbp_chr17_18183723_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 61.74 Mbp_chr1_61742720_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 61.74 Mbp_chr1_61742720_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 99.10 Mbp_chr15_99104971_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 99.10 Mbp_chr15_99104971_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 45.98 Mbp_chr22_45978688_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 45.98 Mbp_chr22_45978688_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 24.68 Mbp_chr13_24680446_F TCGTCGGCAGCGTCTCAATCCTGGACTCTCCCGA 59.6 0.00
mm4__chr13 24.68 Mbp_chr13_24680446_R GTCTCGTGGGCTCGGCACCAGCCTTAAATGCCGC 59.8 0.00
mm4__chr12 27.52 Mbp_chr12_27524053_F TCGTCGGCAGCGTCGGCTCAGCGAGGAATGCATA 60.2 0.00
mm4__chr12 27.52 Mbp_chr12_27524053_R GTCTCGTGGGCTCGGCCCTGCAGGAATCAGCCAAA 60.6 0.00
mm3__chr5 171.42 Mbp_chr5_171419693_F Primer3: not found at this Tm N.d. 0.00
mm3__chr5 171.42 Mbp_chr5_171419693_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 113.60 Mbp_chr2_113602186_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 113.60 Mbp_chr2_113602186_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 206.26 Kbp_chr8_206260_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 206.26 Kbp_chr8_206260_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 45.73 Mbp_chr12_45730362_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 45.73 Mbp_chr12_45730362_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 18.23 Mbp_chr19_18225716_F TCGTCGGCAGCGTCTGGACCGAGTGCTTAGAGGA 59.9 0.00
mm4__chr19 18.23 Mbp_chr19_18225716_R GTCTCGTGGGCTCGGATGAACTCTGGGTGTCCACG 59.6 0.00
mm4__chr7 150.37 Mbp_chr7_150372655_F TCGTCGGCAGCGTCCCTTCGTGTGTCCCGACTG 60.3 0.00
mm4__chr7 150.37 Mbp_chr7_150372655_R GTCTCGTGGGCTCGGCTTCTGGCTGAAGGCTTTGC 59.7 0.00
mm4__chr7 103.15 Mbp_chr7_103149225_F TCGTCGGCAGCGTCAGGTCCAACAGAAACCACGG 60.1 0.00
mm4__chr7 103.15 Mbp_chr7_103149225_R GTCTCGTGGGCTCGGCAAGCGCGAGGTGAGTCC 60.8 0.00
mm4__chr4 54.23 Mbp_chr4_54233937_F TCGTCGGCAGCGTCTCGCCAGGCTGGTTTATGG 60.0 0.00
mm4__chr4 54.23 Mbp_chr4_54233937_R GTCTCGTGGGCTCGGTCTCGCCACCTCAGCATTTC 60.3 0.00
mm4__chr19 56.66 Mbp_chr19_56664220_F TCGTCGGCAGCGTCGCTTCTCGCCTGTGTGGAT 60.0 0.00
mm4__chr19 56.66 Mbp_chr19_56664220_R GTCTCGTGGGCTCGGCCTACACGTGCCAGGACTG 60.0 0.00
mm4__chr17 67.99 Mbp_chr17_67993581_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 67.99 Mbp_chr17_67993581_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 50.06 Mbp_chr12_50057721_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 50.06 Mbp_chr12_50057721_R Primer3: not found at this Tm N.d. 0.00
mm3__chr2 151.83 Mbp_chr2_151828529_F TCGTCGGCAGCGTCGCCTGGAGGGACGTGAGT 60.9 0.00
mm3__chr2 151.83 Mbp_chr2_151828529_R GTCTCGTGGGCTCGGAGCCTCAGTCCCAGGGTAAA 60.1 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

mm4__chr19 9.83 Mbp_chr19_9834992 CATAAGCTCTATCCCGCCTGGGTGGGCTCTGCGGCGACGCGCGCCAAGAAGGGGTCGGGAGTTTTTTGGCGCTCGCTAAA
GGGCGTTGAGAAGGGC
mm4__chr22 41.67 Mbp_chr22_41666852 GACTTCTACCGCGGCCATCGCGCTTCCATCCGCCAGGTGGCGACACAGCCGGCGCGGAGCGCCGATCTGGGCCTGCGCAT
GCGC
GCCACGGACGAGGCAGGCCGGGTGCCTGGGCCTGGGAGCCATAAGCCGGAGAGTC
mm4__chr22 44.33 Mbp_chr22_44331164 TTCCTCTCCAAGGTTCAGCGACCCCGACCTTGGTGAGCGCCGGCGCGGACCCCAGTGCCGAACACCGGCTGTCCCGCACG
GTTCCTGCCCCCGGACCCTTCCCCGGTCGTCCCCAGCCCCTTTCTAAAGAGCCCCG
mm3__chr11 95.15 Mbp_chr11_95150808 CGGCCGGGAGCTATTACAGCAGGGTCTGCCCGTCCGACGACGGCGGCTCCAATCTGGGGTCCCGCCGGCGCCGACCCCTC
AGCTCTCTCCCGATACCTGCTGGCCTCTCTCA
mm4__chr17 42.42 Mbp_chr17_42422039 GTGTCCTCAAGGGCCTGAAAATGCCTGGTGGCCAGGAGCAGAGAGCAGCGAGGGGACAGGGGTGGGCCGCCGAGGCCGAT
GTGGGGGCGCGGGGGCGC
AGGGTCTGGGGAGGAGGTT
mm3__chr4 1.56 Mbp_chr4_1562486 GAGGCCGGTGTCATTAGTGGTAATGGATGGCCGGCGCGGGCCCAGATAAGACGTCCTTCCACATGGTCAG
mm4__chr5 157.67 Mbp_chr5_157671537 TATGTGTCTCCCAGGCAACGCGCCGCGCTCGGTTCAGGGCTCCGCCCAGGGACTCACTCCGCGTCAGCGCGCGCCCACCT
AGG
TGGGCTGGCAACCAGCCACCAGTTTCCAA
mm4__chr14 60.17 Mbp_chr14_60165310 GTCGCCGTCAGCCCTCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNTCCCAGTTCATTGCGGCCGCGCACGCCCAGCTCGGGGGGAAGAAGACGGCCCGCAC
mm3__chr11 68.84 Mbp_chr11_68843977 CCGCTCCAAGGTCATTTCCTCCCTCGCTCAGCGCCCCGGTGCGCGCCCAGCTGGGATTGGCCCCGCGCCCTGTCTGTTCC
CAGGGCTCTTCGG
mm4__chr22 41.29 Mbp_chr22_41286314 CTCGGGTAGAGTAGTCCGGTTCCCCATCTGGGAGCGCGATGGGGCGCGCACCGCGAACTACCCGTCCCATGGCGCACCGC
GCGCAGAGCGTCCTCCACCGCCCTCCTCTTCGCCCTCCCCTGGTGGCTTCTGGCTCTTTC
mm3__chr13 27.97 Mbp_chr13_27970495 CTGGAAGTGGCCGTCTTGGGGACCGAAGCCGCGCAGCCCGGGATCGGGAGCTGCCCCGACAGGAGCGCGGAGGCGGCGAG
ATTCGGCCCCCGCGCGCGCCCAGCTCGGTTTCAGCAACCGCGTGGAG
mm3__chr4 1.01 Mbp_chr4_1011271 CGGAGGTGTCTCGAGAGTTGAGGACCCGACCCGGGCGCGCGCGGGCGCAGGTATGGGGAGGGCGCGGCGGCTCCTGGCCG
GTCCTAGG
mm4__chr9 91.95 Mbp_chr9_91950673 GAAAACAAACAGGCCGCCTTGGCACCGGCGCCCTCCCAGGTCGGTGGGGACAAGCGCGTCCCCCGAAATGCTCAAAACTG
CCAGGGG
mm3__chr2 45.01 Mbp_chr2_45009481 ATTCTAAGCGGGCATGAGGCGCGGCGGCCCGCGCCCTGATTGGTCCGGTTATCTGACCCGGGGCCTGCCAGCGCCAGACA
ATAGTCGGAGTCAAATTATTCGCCATGGAGACGCTGTCAGTCAC
mm4__chr3 4.30 Mbp_chr3_4303508 GTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCCGCGCCGGCGCCCTTCCAGGTAGGGGCGGGGCCAG
GCGGCGCGGGAGGCGGGCGCGCGGCTCCCCCACGTGGTCTCCTCAAG
mm4__chr14 69.48 Mbp_chr14_69484560 CCTTCTGGACGCACGGAGCCCGGCCCTCCAGCCGGCGCCCGCCCTGATTGGCGCCCGAGGCCGGCCTCCCGCCGGCTCAC
CTGCCACTCCCAGCCAATCGGGGCCACCAGAATT
mm3__chr16 229.48 Kbp_chr16_229483 CCACTCGGCTCTTTCCCGCCGCGGGGGACGCCGGGAGGAAGTACGACGGCGCCGGCGCACGCCCTGCTGGGAAATGTAGT
TTCTGGGGTGGGTGCTTAGGGAGGCTGAGGCTCTGAGTGGTCGCAAGTTGTCCAAC
mm4__chr16 400.82 Kbp_chr16_400824 GATGTATGAGCCAGGCAGGGGTCCAGAGACCACCAGCCTGCAATCCGGAGAACCCAAGCTAGGCGCGAGCCGGTGCTGTG
ATCCCGCCAAAGGAGAA
mm4__chr17 64.13 Mbp_chr17_64129866 GGCTCACGGGGCATCTGGCCGCCGCCGTCGCGCTCCCAGCTCGGACTCCAGCCCCGGCGCCGCGACGCTGCCCCGGCCCC
GACCCGGCCGTCGCGAGCTGTCCTCCACCCCAC
mm4__chr9 114.40 Mbp_chr9_114398497 TGGTTTCCTGGAGTTGAGCGGCAAACAAAGGCCTCCAATCCGGGCGCGGGGCGGGGCGGTGGCTCCTTTGCATAATCCCT
GAGGCCC
mm4__chr12 120.45 Mbp_chr12_120446514 GGGGCCAAGGAAGGAAGAAATGTGGTCGCGGTTGGTGTGGCTGGGCCTTCGGGCCCCTCTGGGCGGGCGCCAGGGCTTCA
CCTCCAAGGCGGATCCTCAGGTAAAGG
mm4__chr14 103.06 Mbp_chr14_103057407 CCATGGGCCGCTCTCCTCCCCTCGGCGCCGCCGCCCTCCCAGCTCGGGCGGCCCGCCCCCGCCGCCCTCAGCCCCGCCCG
CGGCCGCGCCCTCCCCGCCGCCGCCGCCGCAGACTAGGAGCGGCGAGGA
mm4__chr12 106.59 Mbp_chr12_106586235 CAGTCGACGCACCTTCTGGCCGGTGCGCGGTTTGCAGTAAGCGCAGGCGCGCGTCCCGCTCGGCCCGCGCTACAGCCCCA
CGCCGCGCAAAAGTGGGTGCTGAGC
mm4__chr22 46.10 Mbp_chr22_46104463 TTGGAGAGGCCTGGGAGGGAGGGCGGCCATGGCCTGCCTGCCTGCCCTTGGCGGCCTGGACAGAGCCCAGCGCCGGCCCC
CGCCCAGTCTGG
CCTTGGCCGTGGCTGCCCCAGGCTTGGCACCAGCCAGCCTTTGG
mm4__chr11 113.45 Mbp_chr11_113452471 TCCATGTTGAGGAGCAAGGGCTAGGGCCACTGGTGTGCATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNACATTGG
GGGACAGGAAATGAGACACTGCTGATTCAGATCCACCC
mm4__chr19 55.38 Mbp_chr19_55384373 CAGAGAGAGCGTTCCGGGGTGCGGCCCGGTCCGGGGCGGGGTCAGGGGACGGCGCCAGGTGTGCCAAGCTCCCGCCTGGT
CACCTGAACCGGGCTGGGCGCGAACCGGCGCGGCCGGGGTTACCCTGGTCGTGGTTTCC
mm4__chr17 81.96 Mbp_chr17_81959973 TGTGGTCCGTGGAAAATCCCCACGTTCCGCCCGGCGCGGGTCCCGCTGTCACCTCGGCGCGGGCGGCGGTCCTCCCGGGG
CGCGCGGCGGCGC
TTGAAGCGTGAAACGCGTCCGCACTGAA
mm3__chr17 81.94 Mbp_chr17_81937350 GTACCCACCCCTCAGCGCTGCGGCCGCCACGCGGCACCCGCACCCAGGCCCCGCCCCCGTCGCGCCAGCGAATCCGGGCG
CGGCCGCCAGGCGCTGGCCAATCAGAGCGCGCGGCGGCGCCAGAGCGGTTGCATCATCTG
mm4__chr1 1.00 Mbp_chr1_1001872 CGAGCACTTAAGCCCCGGCTCTCCTGCCCCGACCTCTCTGCGCGCGCCTCGGCGCTGGAGAGGCTCGGACCGGCCCCGTA
GCCCAGGAGGTGGTGTGGATGGTGTC
mm4__chr18 47.93 Mbp_chr18_47931326 CCTCCCCAGTCTAGCGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGCGGTTTCGCTTGAGTTGGGCGCC
GGCGCGCTCCCACCGGGG
CTCACGGGCGGAGAGGGAGAG
mm4__chr18 75.64 Mbp_chr18_75639051 GGCAGGACTCTCCACTTGTCACTGCTTCAAATCTTCTTTGGGAGAGACAGTGCTGGAAGCGCCGGCTCTCGCCCAGCCCG
G
ATGAGAGCACTGGGACAGATTGATGACCTCAGCCCTCATCG
mm4__chr14 22.84 Mbp_chr14_22837361 CATGCGCGAGAGTTTGTTGTTTCTTCGACTTCCCAGCTCTCCAGCGCTCTCCCGCCGGCGCGCCCTCCGATGGGCGCTTC
GGCCTCCCCGGGTGGCCCAGGCCTTCAGCGGACGAGTCTG
mm4__chr13 24.68 Mbp_chr13_24680446 TCAATCCTGGACTCTCCCGACCCCTAGCTGTCGGTCCCCCTCCCTGCGCGCGCGCCGGCGGGTTTCCTACCCTCCGAGGC
GTCCGCTGGCCTGCGCCCTGGCGGGGACGTGGGCGGGGCGGGCGGCATTTAAGGCTGGTG
mm4__chr12 27.52 Mbp_chr12_27524053 GGCTCAGCGAGGAATGCATAAAATTAGTGGACTCCTCTGAGTGACGTTCCCCGGCTCCAATCAGCGGGCGCGCAGGCGCC
CCCGGCCCCGCCCCACTGCTGACTCTCTTTGGCTGATTCCTGCAGGG
mm4__chr19 18.23 Mbp_chr19_18225716 TGGACCGAGTGCTTAGAGGATGCAGCGCAAGCAGCTGCCGGGCGCAGGCGCGCGCTCAGCCTGGGACTCCCGAACGCCCT
GCCTCGAAAACCCCCTCAATTCAGGTCTTCCTCCGTGGACACCCAGAGTTCAT
mm4__chr7 150.37 Mbp_chr7_150372655 CCTTCGTGTGTCCCGACTGCGGCAAGGCCTTCCGCCACAAACCCTACCTGGCGGCGCACCGGCGCATCCACACCGGCGAG
AAGCCCTACGTCTGCCCCGACTGCGGCAAAGCCTTCAGCCAGAAG
mm4__chr7 103.15 Mbp_chr7_103149225 AGGTCCAACAGAAACCACGGAGCTCCGGACACTCGGGATCCCGGGCCGCGGGCGCGCGGCCAGAGCGGCGGGGTGCGAGC
GCGGGGGTGCGGACTCACCTCGCGCTTG
mm4__chr4 54.23 Mbp_chr4_54233937 TCGCCAGGCTGGTTTATGGGGGCGGCGGTGGCGCGCGGACAGATAGGACAGCGCGGCCCGGGAACGGCGTCGAGGTCCAG
GCGGTCCCCCTGCCCCGAGGGGGCGGAAATGCTGAGGTGGCGAGA
mm4__chr19 56.66 Mbp_chr19_56664220 GCTTCTCGCCTGTGTGGATGCGCCGGTGCTCGGCCAGAGAGGCGCTCTGGCTGAAGAGCGCGCCGCAGTCCTGGCACGTG
TAGG
mm3__chr2 151.83 Mbp_chr2_151828529 GCCTGGAGGGACGTGAGTGCGCAGGCGCGCGGCCAGAGGGGCGGAGCGTAGGAAAGGGCGCCCGCCCTGTCCTCGCTTAT
TTGCATAACCAAGACTGCGGAAGGCGCAGGACTTTACCCTGGGACTGAGGCT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.