← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529712_F | TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC | 60.1 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529712_R | GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT | 60.6 | 1.00 |
| mm4__chr1 167.49 Mbp_chr1_167492268_F | TCGTCGGCAGCGTCGCGTGCTCACAGTTGACATG | 60.1 | 0.42 |
| mm4__chr1 167.49 Mbp_chr1_167492268_R | GTCTCGTGGGCTCGGGTCTCCTGGTCTAGTTGGCG | 59.8 | 0.42 |
| mm4__chr2 36.67 Mbp_chr2_36671112_F | TCGTCGGCAGCGTCACCACACTTCTGTAAGATGAGTC | 57.8 | 0.29 |
| mm4__chr2 36.67 Mbp_chr2_36671112_R | GTCTCGTGGGCTCGGTCTGTCCTCATTTTGGGTGCA | 59.8 | 0.29 |
| mm4__chr6 30.07 Mbp_chr6_30067205_F | TCGTCGGCAGCGTCGAACTAATCCAGCGACGCCT | 60.1 | 0.20 |
| mm4__chr6 30.07 Mbp_chr6_30067205_R | GTCTCGTGGGCTCGGCCCTGGGGATAACCTGGCTA | 60.4 | 0.20 |
| mm4__chr17 82.02 Mbp_chr17_82023286_F | TCGTCGGCAGCGTCCTTTGTTCTCATTGGCCCGC | 60.1 | 0.19 |
| mm4__chr17 82.02 Mbp_chr17_82023286_R | GTCTCGTGGGCTCGGGGGGCCTCAGTATCCAGGA | 60.0 | 0.19 |
| mm4__chr8 3.59 Mbp_chr8_3585073_F | TCGTCGGCAGCGTCACAACTCGCTATCAGCTGGG | 59.8 | 0.19 |
| mm4__chr8 3.59 Mbp_chr8_3585073_R | GTCTCGTGGGCTCGGACCACTAGGGGTCAGTCACA | 59.8 | 0.19 |
| mm4__chrX 149.53 Mbp_chrX_149526013_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chrX 149.53 Mbp_chrX_149526013_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr1 7.77 Mbp_chr1_7771217_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr1 7.77 Mbp_chr1_7771217_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr9 37.80 Mbp_chr9_37800772_F | TCGTCGGCAGCGTCGTGTCTCAGCTTTCCCGGTC | 60.6 | 0.12 |
| mm4__chr9 37.80 Mbp_chr9_37800772_R | GTCTCGTGGGCTCGGCCTCAGTGCCCCTGTTCAG | 60.0 | 0.12 |
| mm4__chr7 2.69 Mbp_chr7_2688085_F | TCGTCGGCAGCGTCTTTGGCTTTAGGCTCTGGGG | 59.9 | 0.12 |
| mm4__chr7 2.69 Mbp_chr7_2688085_R | GTCTCGTGGGCTCGGCAGGACAGGAGGGCCACA | 60.9 | 0.12 |
| mm4__chr3 11.02 Mbp_chr3_11023589_F | TCGTCGGCAGCGTCGGGAACTTCTGGACCCACTG | 59.9 | 0.10 |
| mm4__chr3 11.02 Mbp_chr3_11023589_R | GTCTCGTGGGCTCGGTTAGACTGGGAGATACTTGTGC | 57.2 | 0.10 |
| mm3__chr19 50.15 Mbp_chr19_50147962_F | TCGTCGGCAGCGTCCTTCCTAGGGATCGCAGCAG | 59.9 | 0.10 |
| mm3__chr19 50.15 Mbp_chr19_50147962_R | GTCTCGTGGGCTCGGTCATTCCCTGAGTCCCACCT | 59.8 | 0.10 |
| mm3__chr5 74.14 Mbp_chr5_74143932_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3__chr5 74.14 Mbp_chr5_74143932_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr4 48.34 Mbp_chr4_48341908_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr4 48.34 Mbp_chr4_48341908_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr19 49.12 Mbp_chr19_49119873_F | TCGTCGGCAGCGTCTCCCGGATTCCCTTCCTCAT | 60.0 | 0.09 |
| mm4__chr19 49.12 Mbp_chr19_49119873_R | GTCTCGTGGGCTCGGCGGGATTCTAGGGTTCGGTC | 59.6 | 0.09 |
| mm4__chr10 81.87 Mbp_chr10_81874994_F | TCGTCGGCAGCGTCGAGGATGTCGGCGCTCCTG | 62.7 | 0.07 |
| mm4__chr10 81.87 Mbp_chr10_81874994_R | GTCTCGTGGGCTCGGCCTCCGGTCGGTCAGAGA | 60.0 | 0.07 |
| mm4__chrX 130.06 Mbp_chrX_130060695_F | TCGTCGGCAGCGTCCCATCGCTGGCTCGACAG | 60.5 | 0.06 |
| mm4__chrX 130.06 Mbp_chrX_130060695_R | GTCTCGTGGGCTCGGTGAGTGAGCTCCTTCCCCTC | 60.6 | 0.06 |
| mm4__chr1 154.57 Mbp_chr1_154567762_F | TCGTCGGCAGCGTCCCTACCACGGCAGAACCAAT | 60.0 | 0.05 |
| mm4__chr1 154.57 Mbp_chr1_154567762_R | GTCTCGTGGGCTCGGGGCTGCTCTTCCAGGGAATA | 59.1 | 0.05 |
| mm4__chr17 45.78 Mbp_chr17_45783357_F | TCGTCGGCAGCGTCAGCCAATTCAGAAGACGGCC | 60.6 | 0.04 |
| mm4__chr17 45.78 Mbp_chr17_45783357_R | GTCTCGTGGGCTCGGGAACGCTGCTAGGAGCCC | 60.2 | 0.04 |
| mm4__chr15 77.63 Mbp_chr15_77631250_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr15 77.63 Mbp_chr15_77631250_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 11.93 Mbp_chr2_11925893_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 11.93 Mbp_chr2_11925893_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr9 93.92 Mbp_chr9_93921356_F | TCGTCGGCAGCGTCCCTCTGCGTTTCCAAGTTGC | 60.0 | 0.04 |
| mm4__chr9 93.92 Mbp_chr9_93921356_R | GTCTCGTGGGCTCGGGCACTGGGTGTGGTTTTGTG | 60.1 | 0.04 |
| mm4__chr9 137.59 Mbp_chr9_137590329_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr9 137.59 Mbp_chr9_137590329_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr11 1.04 Mbp_chr11_1037090_F | TCGTCGGCAGCGTCCGGGATGGACCTGTTGGG | 59.7 | 0.02 |
| mm4__chr11 1.04 Mbp_chr11_1037090_R | GTCTCGTGGGCTCGGATTCAGGTGCAGCAGGGTTC | 60.6 | 0.02 |
| mm4__chr14 32.93 Mbp_chr14_32933398_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr14 32.93 Mbp_chr14_32933398_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chr14 56.12 Mbp_chr14_56119059_F | TCGTCGGCAGCGTCGAAGTGGCCAGAACTCGGG | 60.3 | 0.02 |
| mm3__chr14 56.12 Mbp_chr14_56119059_R | GTCTCGTGGGCTCGGCACATGGAGTCCCTGCCC | 59.7 | 0.02 |
| mm4__chrX 9.47 Mbp_chrX_9465457_F | TCGTCGGCAGCGTCCACAAGTTGCGCTGCTCG | 60.1 | 0.02 |
| mm4__chrX 9.47 Mbp_chrX_9465457_R | GTCTCGTGGGCTCGGGCCTCCCCGCAGAAGTTT | 59.9 | 0.02 |
| mm4__chr2 239.35 Mbp_chr2_239351879_F | TCGTCGGCAGCGTCTGTGGACGCAAGACAGAAGT | 59.5 | 0.01 |
| mm4__chr2 239.35 Mbp_chr2_239351879_R | GTCTCGTGGGCTCGGCAGAGCCAGTCCTGTGCTG | 60.3 | 0.01 |
| mm4__chr1 6.26 Mbp_chr1_6260748_F | TCGTCGGCAGCGTCCGATAGGGCGCGGAAAGC | 61.3 | 0.01 |
| mm4__chr1 6.26 Mbp_chr1_6260748_R | GTCTCGTGGGCTCGGCGCTCGAATGCAGGGTGA | 60.1 | 0.01 |
| mm4__chr15 42.02 Mbp_chr15_42024149_F | TCGTCGGCAGCGTCTAAGTGGGATGGGAGGGGAG | 60.0 | 0.01 |
| mm4__chr15 42.02 Mbp_chr15_42024149_R | GTCTCGTGGGCTCGGGGGGATGTCACTGTTTGCCT | 60.2 | 0.01 |
| mm4__chr1 157.99 Mbp_chr1_157992657_F | TCGTCGGCAGCGTCGGGATGGGTGGAAGGCTAAG | 59.8 | 0.01 |
| mm4__chr1 157.99 Mbp_chr1_157992657_R | GTCTCGTGGGCTCGGCTCTTGGACCTGCCTTGAGG | 60.0 | 0.01 |
| mm4__chr18 61.33 Mbp_chr18_61334100_F | TCGTCGGCAGCGTCCTCTCTGGAAGCAGGGAAGC | 60.1 | 0.01 |
| mm4__chr18 61.33 Mbp_chr18_61334100_R | GTCTCGTGGGCTCGGTGGATCCCCAGTCACCATCC | 60.9 | 0.01 |
| mm4__chr20 56.63 Mbp_chr20_56630187_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr20 56.63 Mbp_chr20_56630187_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chrX 14.53 Mbp_chrX_14529596_F | TCGTCGGCAGCGTCCCAGCACAGGATCTCAGCAA | 60.0 | 0.01 |
| mm4__chrX 14.53 Mbp_chrX_14529596_R | GTCTCGTGGGCTCGGTCTTTTCTGTTTAGCAAAGCCCT | 58.7 | 0.01 |
| mm3__chr18 72.87 Mbp_chr18_72867651_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr18 72.87 Mbp_chr18_72867651_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr17 81.44 Mbp_chr17_81442566_F | TCGTCGGCAGCGTCTTCGTGCCTTCTGTGGGAC | 59.9 | 0.00 |
| mm4__chr17 81.44 Mbp_chr17_81442566_R | GTCTCGTGGGCTCGGGAGACTCCATAGGGTGCAGC | 59.8 | 0.00 |
| mm4__chr11 31.99 Mbp_chr11_31987512_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 31.99 Mbp_chr11_31987512_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 31.90 Mbp_chr6_31902066_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 31.90 Mbp_chr6_31902066_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 158.56 Mbp_chr6_158560014_F | TCGTCGGCAGCGTCCTCAGGGCTAGCCTGGGT | 60.3 | 0.00 |
| mm4__chr6 158.56 Mbp_chr6_158560014_R | GTCTCGTGGGCTCGGCGGAAGCGACTCTCACGG | 60.2 | 0.00 |
| mm4__chr3 138.07 Mbp_chr3_138068633_F | TCGTCGGCAGCGTCCTCCAGGGTCGTGTTGTCAT | 59.6 | 0.00 |
| mm4__chr3 138.07 Mbp_chr3_138068633_R | GTCTCGTGGGCTCGGGGTAGCAGCCTTGGTGAAGT | 59.9 | 0.00 |
| mm4__chr2 232.49 Mbp_chr2_232487829_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 232.49 Mbp_chr2_232487829_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 42.70 Mbp_chr19_42698514_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 42.70 Mbp_chr19_42698514_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 44.54 Mbp_chr17_44539941_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 44.54 Mbp_chr17_44539941_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 58.03 Mbp_chr16_58026809_F | TCGTCGGCAGCGTCTACATTCAGCCTGTGTCCCG | 59.7 | 0.00 |
| mm4__chr16 58.03 Mbp_chr16_58026809_R | GTCTCGTGGGCTCGGGAGGGAAGGAAGGAGGGGAG | 60.6 | 0.00 |
| mm4__chr16 30.33 Mbp_chr16_30334910_F | TCGTCGGCAGCGTCGTGGGTGGAGGGCCTAGG | 61.0 | 0.00 |
| mm4__chr16 30.33 Mbp_chr16_30334910_R | GTCTCGTGGGCTCGGGGAGCTGGAATGACTGGCAA | 60.3 | 0.00 |
| mm4__chr16 29.59 Mbp_chr16_29594822_F | TCGTCGGCAGCGTCGTGGGTGGAGGGCCTAGG | 61.0 | 0.00 |
| mm4__chr16 29.59 Mbp_chr16_29594822_R | GTCTCGTGGGCTCGGGGAGCTGGAATGACTGGCAA | 60.3 | 0.00 |
| mm4__chr16 22.44 Mbp_chr16_22437533_F | TCGTCGGCAGCGTCCTGGAATGACTGGCAACCCA | 60.2 | 0.00 |
| mm4__chr16 22.44 Mbp_chr16_22437533_R | GTCTCGTGGGCTCGGGGAGGAGACCCAGGAAGCT | 60.3 | 0.00 |
| mm4__chr16 18.93 Mbp_chr16_18925885_F | TCGTCGGCAGCGTCGAGGGGTGGAGGGCCTAG | 60.4 | 0.00 |
| mm4__chr16 18.93 Mbp_chr16_18925885_R | GTCTCGTGGGCTCGGCTGGAATGACTGGCAACCCA | 60.2 | 0.00 |
| mm4__chr11 60.92 Mbp_chr11_60924401_F | TCGTCGGCAGCGTCCCTCTGTTGCTTCCAGGGAC | 60.3 | 0.00 |
| mm4__chr11 60.92 Mbp_chr11_60924401_R | GTCTCGTGGGCTCGGAATCGCAGCACATGGGCT | 60.0 | 0.00 |
| mm4__chr1 43.43 Mbp_chr1_43427702_F | TCGTCGGCAGCGTCCAGACAGAGAGTGCGGATGG | 60.1 | 0.00 |
| mm4__chr1 43.43 Mbp_chr1_43427702_R | GTCTCGTGGGCTCGGAAGGCACTCACCGGAAGATG | 60.0 | 0.00 |
| mm4__chr1 232.81 Mbp_chr1_232805709_F | TCGTCGGCAGCGTCCGCTGTTGGTTTACCCTCCT | 59.9 | 0.00 |
| mm4__chr1 232.81 Mbp_chr1_232805709_R | GTCTCGTGGGCTCGGGTGCAGGGTAGCAGGCTG | 60.4 | 0.00 |
| mm3__chr7 35.04 Mbp_chr7_35036979_F | TCGTCGGCAGCGTCAAAAGTCGGAGAGGGCTGC | 60.0 | 0.00 |
| mm3__chr7 35.04 Mbp_chr7_35036979_R | GTCTCGTGGGCTCGGGGGACGCGGAACTTTGAGG | 61.0 | 0.00 |
| mm3__chr3 51.39 Mbp_chr3_51391273_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr3 51.39 Mbp_chr3_51391273_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529712 |
TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG |
| mm4__chr1 167.49 Mbp_chr1_167492268 |
GCGTGCTCACAGTTGACATGTGTGAAGGACCAGGAGAGCCGGAAGAGGTCAGCAGGCTCAGGGGGTGCTGTTAGCCTGTT TCACCGACAGAAAGGCTTAACCCCTGCGGCGCCAACTAGACCAGGAGAC |
| mm4__chr2 36.67 Mbp_chr2_36671112 |
ACCACACTTCTGTAAGATGAGTCAGGACTGGGAGAGCCAGAAGAGGTTTCCAGGAGGAAGTAAGACTTGATTTGCACCCA AAATGAGGACAGA |
| mm4__chr6 30.07 Mbp_chr6_30067205 |
GAACTAATCCAGCGACGCCTGCGCTTTGACGCATTTGGTGCCGTGGAAGGGAAAAAGGGGGACTGCAGTATGCGTCACAC CCGGAAGCGGCGAGCCGGAAGTGGGGTTAGCCAGGTTATCCCCAGGG |
| mm4__chr17 82.02 Mbp_chr17_82023286 |
CTTTGTTCTCATTGGCCCGCACCAAGCGCTGCCTGCGCAGAGCCTGCTAGGAGTTGTAGTTCCCGCGGCCTCTCCCGGCG CGCCTTGTTCTTCCTGGATACTGAGGCCCC |
| mm4__chr8 3.59 Mbp_chr8_3585073 |
ACAACTCGCTATCAGCTGGGCCATACACCCTCCCTCCCCTTCCGGTGGGGCGGGTCCTGCAAAGCTGTATCCCCTGCATG TGACTGACCCCTAGTGGT |
| mm4__chr9 37.80 Mbp_chr9_37800772 |
GTGTCTCAGCTTTCCCGGTCCCCGGCGGACGGTGGCCGAGGGGCGGCGCGGGTTTCCAGCCGGTGGGGTTAGGCCGCTGC ACGCCGGAAGTGGCAGCTGAACAGGGGCACTGAGG |
| mm4__chr7 2.69 Mbp_chr7_2688085 |
TTTGGCTTTAGGCTCTGGGGCCCCCCTGCCCGGGTGTTTCTGGGAGGCTGGAGGGTCCCACGTGAGCTCCACCGCGGGCA GCTGGAGGGAGTGGGGCAGGACGCTGAGCGCCGGGAGGGGTGTGGCCCTCCTGTCCTG |
| mm4__chr3 11.02 Mbp_chr3_11023589 |
GGGAACTTCTGGACCCACTGGGTTCTCAAATCCAAGGCTGGTGTAAAAGTATCTCTGATCCATTCCTAGGACTAGGGACG CCGGAGGAGGGCACAAGTATCTCCCAGTCTAA |
| mm3__chr19 50.15 Mbp_chr19_50147962 |
CTTCCTAGGGATCGCAGCAGTCCCACGCTCCGCCGGACTCCTGGCAAACAGCAGACGCCCAATTCCTGCGCGCCGAGTCG TCTGAGCCGCACTGATACTAGGTGGGACTCAGGGAATGA |
| mm4__chr19 49.12 Mbp_chr19_49119873 |
TCCCGGATTCCCTTCCTCATCCCAGGGGACCCCCGAGCCCAGATTTCACACCCCAACCCCGGGGACTCAGGCGTGCCCCT CCCGGCGCCCAGAGTCCCCGACCGAACCCTAGAATCCCG |
| mm4__chr10 81.87 Mbp_chr10_81874994 |
GAGGATGTCGGCGCTCCTGGAGCGCACGCAGGCGGCGGNNNNNNNNNNNNNNNNNGGGCGCGGGGACCCGGCGCGCAGGA GGCGGCTTGGAGGGCTGCAGACGCGCCCCGCCGCTCTCTGACCGACCGGAGG |
| mm4__chrX 130.06 Mbp_chrX_130060695 |
CCATCGCTGGCTCGACAGCGCCACCTGCTGGACGTCGGCCTCCACGTCCGGCGCGCCGGGGCCCGAAGTTCTCGGAGGCT GGGACCTCAGTCTGGGAGGGGAAGGAGCTCACTCA |
| mm4__chr1 154.57 Mbp_chr1_154567762 |
CCTACCACGGCAGAACCAATCGAAGACTATCCCAGCCGGGGACCCCCCTTTTTTTTCCTGGGACCCAGCGTTTCCGCCTC CGGGGCGCAGACTCCTCCCCCTCACCGTCCCAATTGTATTCCCTGGAAGAGCAGCC |
| mm4__chr17 45.78 Mbp_chr17_45783357 |
AGCCAATTCAGAAGACGGCCCCGGGGTCTGGCGGGGAGGCCCACCGCTGCCCAGAGGGCTCGGCCGGCCGGGAGGGGGCG CTGTGTCCTCCCCACGCCCTGCCCGCGGGCTCCTAGCAGCGTTC |
| mm4__chr9 93.92 Mbp_chr9_93921356 |
CCTCTGCGTTTCCAAGTTGCATCAGCACCACTAGGACTCCGCCAGCAGGAAGGGGGTGGACCCGAGAGACTTGGCCAGGC CAAGCCATGACCCCATCATTTTGTCTTAACACAAAACCACACCCAGTGC |
| mm4__chr11 1.04 Mbp_chr11_1037090 |
CGGGATGGACCTGTTGGGGGAGGTCGGGGGCCCTGGGGCACCTGCTCTGCCCCTTCCGGGGAGCCCAGTGCTGACCCAGC TTGGGGGGAACCCTGCTGCACCTGAAT |
| mm3__chr14 56.12 Mbp_chr14_56119059 |
GAAGTGGCCAGAACTCGGGCGACCCAGCGGGGGCAGCGCCGGGCACCGGGTCCGCGGCCGTATCGTGGGGGCGGGGGCAG GGGCTCCACTTCCCGCGCGCCGCGTCCGGGCCGGGGAGGGCGGGCAGGGACTCCATGTG |
| mm4__chrX 9.47 Mbp_chrX_9465457 |
CACAAGTTGCGCTGCTCGCGACGAGGTGAGTGCGCAGGGCTCGGCGGGCCGGGCGCGGGGTCCGCAAACTTCTGCGGGGA GGC |
| mm4__chr2 239.35 Mbp_chr2_239351879 |
TGTGGACGCAAGACAGAAGTCGATGGCTAAGTGCCCTTGAAGGCCACGGTGCGCTGGAAGTGGCAGGAAGCTGGGCAGCA CAGGACTGGCTCTG |
| mm4__chr1 6.26 Mbp_chr1_6260748 |
CGATAGGGCGCGGAAAGCCCCCTCCCTTCCCGCCGAGTCCTCCCGCCGGGGTCCTCCCGTCCGTCCGCCCTCGGGCCAGG GCGGCGACGCTCACCCTGCATTCGAGCG |
| mm4__chr15 42.02 Mbp_chr15_42024149 |
TAAGTGGGATGGGAGGGGAGCAGAGCAGAGGACTCGGGGAGTTGGAAGTGGACTGGGACAGTGAGTAAGGAGTGTCAGGC AAACAGTGACATCCCC |
| mm4__chr1 157.99 Mbp_chr1_157992657 |
GGGATGGGTGGAAGGCTAAGATGAACAAAGGAAGGGTCAACTGTGCCTCTTCCAGGCCGCTGAGTCCTCCAACGCCTAGC TGTGCTCAACCTTGACTGGGTGAAGCCAAGGCAGGGCCTCAAGGCAGGTCCAAGAG |
| mm4__chr18 61.33 Mbp_chr18_61334100 |
CTCTCTGGAAGCAGGGAAGCTGGGGCGCACCGGGGCAGGTGGAGGACGTGGCGCGCTGGGAGCGGGGACTGCGCGCGCCT GGGGATGGTGACTGGGGATCCA |
| mm4__chrX 14.53 Mbp_chrX_14529596 |
CCAGCACAGGATCTCAGCAATTTTTCCCTCTTCCCCCCACCCACTCCAGCGCGCAGAGTCCTTTCTTCTCTCTCATTTAC AACTTCTTTTTAAAAGAAAACATTTTCTAGAAAAAGGGCTTTGCTAAACAGAAAAGA |
| mm4__chr17 81.44 Mbp_chr17_81442566 |
TTCGTGCCTTCTGTGGGACACCTGGCCGACAAGGGCCGCCCCTTCCAGGCCGCCGAGGCCTGTGCCGTGGCAGGGGAGGG CAAGGACCGGCACCTGGAGGGAACCATGGCCCCCGACCACGCTGCACCCTATGGAGTCTC |
| mm4__chr6 158.56 Mbp_chr6_158560014 |
CTCAGGGCTAGCCTGGGTGCTCCCGGCCGTGGGGCTCCGCCCCGGCACGGGGCCACGAAGGAGGGCCACNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCCGCCGTGAGAGTCGCTTCCG |
| mm4__chr3 138.07 Mbp_chr3_138068633 |
CTCCAGGGTCGTGTTGTCATTACTGAAGACCAGCAGGAGTCCGCGGGCCTGAAGAGGGGCTCAGTAGTCCTAGATGGACT TCACCAAGGCTGCTACC |
| mm4__chr16 58.03 Mbp_chr16_58026809 | TACATTCAGCCTGTGTCCCGGGAGCCGCTTCCGCCGCCCCCAGCCCTTGCCTCCCCTCCTTCCTTCCCTC |
| mm4__chr16 30.33 Mbp_chr16_30334910 |
GTGGGTGGAGGGCCTAGGCCGCGCCTCCCAGCCCCGCGGCCCTAGGCCTCGGCCCGCCCGAGGCGGAGCCCGGGAGGTCG GGGCGGGGTCCCGGGCCGGTCACCCACCTGGGTTGCCAGTCATTCCAGCTCC |
| mm4__chr16 29.59 Mbp_chr16_29594822 |
GTGGGTGGAGGGCCTAGGCCGCGCCTCCCAGCCCCGCGGCCCTAGGCCTCGGCCCGCCCGAGGCGGAGCCCGGGAGGTCG GGGCGGGGTCCCGGGCCGGTCACCCACCTGGGTTGCCAGTCATTCCAGCTCC |
| mm4__chr16 22.44 Mbp_chr16_22437533 |
CTGGAATGACTGGCAACCCAGGTGGGTGACCGGCCCGGGACCCCGCCCCGACCTCCCGGGCTCCGCCTCGGGCGGGCCGA GGCCTAGGCCCTCCACCCCCGGGAGCCGGGCGCAGCTTCCTGGGTCTCCTCC |
| mm4__chr16 18.93 Mbp_chr16_18925885 |
GAGGGGTGGAGGGCCTAGGCCGCGCCTCCCAGCCCCGCGGCCCTAGGCCTCGGCCCGCCCGAGGCGGAGCCCGGGAGGTC GGGGCGGGGTCCCGGGCCGGTCACCCACCTGGGTTGCCAGTCATTCCAG |
| mm4__chr11 60.92 Mbp_chr11_60924401 |
CCTCTGTTGCTTCCAGGGACGTGGCCCTCTTCCCGACCTCGGTCGCCTGAGCGCGCAGGGATTGGGGATCCGTAGGGCAC TAGGACCCGGCGGGGCGGAGGGGGCAGCCCATGTGCTGCGATT |
| mm4__chr1 43.43 Mbp_chr1_43427702 |
CAGACAGAGAGTGCGGATGGGCCCCGGACCCGGTGTCCTGTCTACATCTACAGCTGTTCACTGGAAGCGCTGAGGGAACA AATGGTTGGCATGCAGCCCCCTCAGGCGCCCCGAGACCTCATCTTCCGGTGAGTGCCTT |
| mm4__chr1 232.81 Mbp_chr1_232805709 |
CGCTGTTGGTTTACCCTCCTGACGGCCCCGGCGCTCCCGCCGCCGAACCGTGGCCCGGTGTCATCCGCTTCGGTCGCGGC AAGTCCTGCCTCTTCCGCCTGCAGCCTGCTACCCTGCAC |
| mm3__chr7 35.04 Mbp_chr7_35036979 |
AAAAGTCGGAGAGGGCTGCAAACTGGCTGGTCCGGCTGGCGGGGCTCGGCGCGGCGGAAGCNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNGCCAGCGCCTCAAAGTTCCGCGTCCC |