CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGGCGCGCGCCCAGATTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	(Wild-type sequence)
211.5	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA-------CAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`7 bp deleted → frameshift`
202.0	`TCCTTAGGCCGCCAGGGGGCGCCGG--------------------------------GCCGGCCGCGTTATTACCATAAA`	`32 bp deleted → frameshift`
199.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC----------------------GGGCCGGCCGCGTTATTACCATAAA`	`22 bp deleted → frameshift`
188.4	`TCCTTAGGCCGCCAGGGGGCGCCGGC-------------------------------------CGCGTTATTACCATAAA`	`37 bp deleted → frameshift`
180.6	`TCCTTAGGCCGCCAGGGGG------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`24 bp deleted → no frameshift`
173.1	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------AAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`11 bp deleted → frameshift`
172.2	`TCCTTAGGCCGCCAGGGG-------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`25 bp deleted → frameshift`
170.8	`TCCTTAGGCCGCCAGGGGGCGCCGG-----------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`17 bp deleted → frameshift`
163.8	`TCCTTAGGCCGCCAGGG--------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`26 bp deleted → frameshift`
162.8	`TCCTTAGGCCGCCAGGGGGCGCCGG------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`18 bp deleted → no frameshift`
156.6	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-------------GAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`13 bp deleted → frameshift`
155.4	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC---------------------------GGCCGCGTTATTACCATAAA`	`27 bp deleted → no frameshift`
149.1	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA--------------AGCCGGGCCGGCCGCGTTATTACCATAAA`	`14 bp deleted → frameshift`
140.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC---------------------GGGCCGGCCGCGTTATTACCATAAA`	`21 bp deleted → no frameshift`
132.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------------TTATTACCATAAA`	`36 bp deleted → no frameshift`
128.1	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-----------------CCGGGCCGGCCGCGTTATTACCATAAA`	`17 bp deleted → frameshift`
127.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC-------------------------------GCGTTATTACCATAAA`	`31 bp deleted → frameshift`
126.8	`TCCTTAGGCCGCCAGGGGG-----------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`23 bp deleted → frameshift`
120.4	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGC------------------------CGGGCCGGCCGCGTTATTACCATAAA`	`24 bp deleted → no frameshift`
120.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------------TTATTACCATAAA`	`38 bp deleted → frameshift`
115.2	`TCCTTAGGCCGCCAGGGGGCGCCGGC---------------------------------CGGCCGCGTTATTACCATAAA`	`33 bp deleted → no frameshift`
114.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG-------------------------GGCCGGCCGCGTTATTACCATAAA`	`25 bp deleted → frameshift`
114.8	`TCCTTAGGCCGCCAGGGGGCGCCGG-------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`25 bp deleted → frameshift`
113.6	`TCCTTAGGCCGCCAGGGGGC---------------------------------------CGGCCGCGTTATTACCATAAA`	`39 bp deleted → no frameshift`
109.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGC----------------------------------GTTATTACCATAAA`	`34 bp deleted → frameshift`
109.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC--------------------------GGCCGCGTTATTACCATAAA`	`26 bp deleted → frameshift`
109.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCGC--------------------------CGGGCCGGCCGCGTTATTACCATAAA`	`26 bp deleted → frameshift`
103.6	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCG---------------------------GGCCGGCCGCGTTATTACCATAAA`	`27 bp deleted → no frameshift`
103.6	`TCCTTAGGCCGCCAGG---------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`27 bp deleted → no frameshift`
103.2	`TCCTTAGGCCGCCAGGGGGCGCCG-----------------------------------------CGTTATTACCATAAA`	`41 bp deleted → frameshift`
98.8	`TCCTTAGGCCGCCAGGGGGCGCCGGC----------------------------CGGGCCGGCCGCGTTATTACCATAAA`	`28 bp deleted → frameshift`
94.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGC-----------------------------CGGCCGCGTTATTACCATAAA`	`29 bp deleted → frameshift`
94.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCG-----------------------------GGCCGGCCGCGTTATTACCATAAA`	`29 bp deleted → frameshift`
91.5	`TCCTTAGGCCGCCAGG----------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`34 bp deleted → frameshift`
89.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC------------------------------GCGTTATTACCATAAA`	`30 bp deleted → no frameshift`
89.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------GCCGCGTTATTACCATAAA`	`30 bp deleted → no frameshift`
84.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGC-------------------------------CGGCCGCGTTATTACCATAAA`	`31 bp deleted → frameshift`
84.8	`TCCTTAGGCCGCCAGGGGG-------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`31 bp deleted → frameshift`
84.0	`TCCTTAGGCCG---------------------------------------------GGCCGGCCGCGTTATTACCATAAA`	`45 bp deleted → no frameshift`
82.0	`TCCTTAGGCCG--------------------------------------------------GCCGCGTTATTACCATAAA`	`50 bp deleted → frameshift`
81.0	`TCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------------TTATTACCATAAA`	`40 bp deleted → frameshift`
81.0	`TCCTTAGGCCGCCAGGGG----------------------------------------CCGGCCGCGTTATTACCATAAA`	`40 bp deleted → frameshift`
80.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------GCCGCGTTATTACCATAAA`	`32 bp deleted → frameshift`
80.8	`TCCTTAGGCCGCCAGGGG--------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`32 bp deleted → frameshift`
80.4	`TCCTTAGGCCGC------------------------------------------------------GTTATTACCATAAA`	`54 bp deleted → no frameshift`
77.4	`TCCTTAGGCCGCCAGGG-----------------------------------------CCGGCCGCGTTATTACCATAAA`	`41 bp deleted → frameshift`
76.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGC---------------------------------CGCGTTATTACCATAAA`	`33 bp deleted → no frameshift`
76.8	`TCCTTAGGCCGCCAGGG---------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`33 bp deleted → no frameshift`
73.2	`TCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------GCCGCGTTATTACCATAAA`	`34 bp deleted → frameshift`
73.2	`TCCTTAGGCCGCCAGGGGGC----------------------------------CGGGCCGGCCGCGTTATTACCATAAA`	`34 bp deleted → frameshift`
73.2	`TCCTTAGG----------------------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`34 bp deleted → frameshift`
73.2	`TCCTTAGGCCGCC------------------------------------------GGGCCGGCCGCGTTATTACCATAAA`	`42 bp deleted → no frameshift`
69.6	`TCCTTAGGCCGCCAGGGGGC-------------------------------------------CGCGTTATTACCATAAA`	`43 bp deleted → frameshift`
69.6	`TCCTTAGGCCGCCAGGGGGCGCCGGCGC-----------------------------------CGCGTTATTACCATAAA`	`35 bp deleted → frameshift`
69.6	`TCCTTAGGCCGCCAGGGGGCG-----------------------------------GGCCGGCCGCGTTATTACCATAAA`	`35 bp deleted → frameshift`
69.6	`TCCTTAGG-----------------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`35 bp deleted → frameshift`
66.6	`TCCTTAGGCCGCCAGGGGGCGC--------------------------------------------GTTATTACCATAAA`	`44 bp deleted → frameshift`
64.5	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------------------------------------TAAA`	`41 bp deleted → frameshift`
61.0	`TCCTTAGG------------------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAA`	`42 bp deleted → no frameshift`
60.6	`TCCTTAGGCCGCCA--------------------------------AAGGAAGCCGGGCCGGCCGCGTTATTACCATAAA`	`32 bp deleted → frameshift`
60.0	`TCCTTAGGCCGCCAGGGGGCG----------------------------------------------TTATTACCATAAA`	`46 bp deleted → frameshift`
60.0	`TCCTTAGGCCGCCAGGGGG--------------------------------------GCCGGCCGCGTTATTACCATAAA`	`38 bp deleted → frameshift`
57.6	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT---------------------------------ACCATAAA`	`33 bp deleted → no frameshift`
57.0	`TCCTTAGGCCGCC-----------------------------------------------GGCCGCGTTATTACCATAAA`	`47 bp deleted → frameshift`
54.0	`TCCTTAGGCCGCCAGGGGGCG----------------------------------------GCCGCGTTATTACCATAAA`	`40 bp deleted → frameshift`
48.8	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC------------------------------------------ATAAA`	`42 bp deleted → no frameshift`
48.8	`TCCTTAGGCCGCCAGG------------------------------------------CCGGCCGCGTTATTACCATAAA`	`42 bp deleted → no frameshift`
46.8	`TCCTTAGGCCGCC---------------------------------------------------GCGTTATTACCATAAA`	`51 bp deleted → no frameshift`
46.4	`TCCTTAGGCCGCCAGGGGGCGCCG-------------------------------------------TTATTACCATAAA`	`43 bp deleted → frameshift`
45.0	`TCCTTAGGCCGCCAG--------------------------------------CCGGGCCGGCCGCGTTATTACCATAAA`	`38 bp deleted → frameshift`
44.6	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT------------------------------ATTACCATAAA`	`30 bp deleted → no frameshift`
44.4	`TCCTTAGGCCGCCAGGGG--------------------------------------------CCGCGTTATTACCATAAA`	`44 bp deleted → frameshift`
42.0	`TCCTTAGGCCGCCAGGG---------------------------------------------CCGCGTTATTACCATAAA`	`45 bp deleted → no frameshift`
40.0	`TCCTTAGGCCGCCAGG----------------------------------------------CCGCGTTATTACCATAAA`	`46 bp deleted → frameshift`
34.4	`TCCTTAGG-------------------------------------------------GCCGGCCGCGTTATTACCATAAA`	`49 bp deleted → frameshift`
30.0	`TCCTTAG----------------------------------------------CCGGGCCGGCCGCGTTATTACCATAAA`	`46 bp deleted → frameshift`
29.6	`TCCTTAGGCCGCCAGGGGGCGCC----------------------------------------------------ATAAA`	`52 bp deleted → frameshift`
29.6	`TCC----------------------------------------------------GGGCCGGCCGCGTTATTACCATAAA`	`52 bp deleted → frameshift`
28.4	`TCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGAT---------------------------------------AAA`	`39 bp deleted → no frameshift`
24.4	`TCCTTAGGCCG--------------------------------------------------------TTATTACCATAAA`	`56 bp deleted → frameshift`
23.2	`TCCTTAGGC---------------------------------------------------------GTTATTACCATAAA`	`57 bp deleted → no frameshift`
23.2	`TCC---------------------------------------------------------GGCCGCGTTATTACCATAAA`	`57 bp deleted → no frameshift`
22.5	`TCCTTAGGCCGCCA--------------------------------------------------------------TAAA`	`62 bp deleted → frameshift`
18.8	`TCC-------------------------------------------------------------GCGTTATTACCATAAA`	`61 bp deleted → frameshift`
15.6	`TCCTTAGGCC-----------------------------------------------------------------ATAAA`	`65 bp deleted → frameshift`
12.3	`TCCTTA----------------------------------------------------------------TTACCATAAA`	`64 bp deleted → frameshift`
10.8	`TCC------------------------------------------------------------------------ATAAA`	`72 bp deleted → no frameshift`
10.5	`TCCTTA-------------------------------------------------------------------CCATAAA`	`67 bp deleted → frameshift`
5.4	`TCCTTA------------------------------------------------------------------------AA`	`72 bp deleted → no frameshift`

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