CRISPOR

Micro-homology scoring of potential deletions

Guide: GCTATTCTCGCAGCTCACCA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTC`	(Wild-type sequence)
200.9	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGC-------------------------CGCGCTCGTCGTCGACAACGGCTC`	`25 bp deleted → frameshift`
181.3	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTC---------------------------GTCGTCGACAACGGCTC`	`27 bp deleted → no frameshift`
162.8	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACC------------------GCGCTCGTCGTCGACAACGGCTC`	`18 bp deleted → no frameshift`
148.2	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGC----------------------------GCTCGTCGTCGACAACGGCTC`	`28 bp deleted → frameshift`
133.8	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGC------------------------------TCGTCGTCGACAACGGCTC`	`30 bp deleted → no frameshift`
133.2	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGC----------------------CGCGCTCGTCGTCGACAACGGCTC`	`22 bp deleted → frameshift`
133.2	`CTCGGCTCACAGCGCGCCCGG----------------------ATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTC`	`22 bp deleted → frameshift`
128.1	`CTCGGCTCACAGCGCGCCCGGCTATTCTCG----------------------------------TCGTCGACAACGGCTC`	`34 bp deleted → frameshift`
122.1	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTC------------------GCCGCGCTCGTCGTCGACAACGGCTC`	`18 bp deleted → no frameshift`
114.8	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGC-------------------------GCTCGTCGTCGACAACGGCTC`	`25 bp deleted → frameshift`
113.6	`CTCGGCTCACAGCGCGCC---------------------------------------GCGCTCGTCGTCGACAACGGCTC`	`39 bp deleted → no frameshift`
111.0	`CTCGGCTCACAGCGCGC--------------------------------------------TCGTCGTCGACAACGGCTC`	`44 bp deleted → frameshift`
90.0	`CTCGGCTCACAGCGCGCCCG--------------------------------------CGCTCGTCGTCGACAACGGCTC`	`38 bp deleted → frameshift`
80.0	`CTCGGCTCACAGCGC----------------------------------------------TCGTCGTCGACAACGGCTC`	`46 bp deleted → frameshift`
78.5	`CTCGGCTCACAGCGCGCCCGGCTATTCTCG-------------------------------------TCGACAACGGCTC`	`37 bp deleted → frameshift`
77.7	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTC--------------------------------------------`	`44 bp deleted → frameshift`
77.7	`CTCGGCTCACAGCGCGCCCGGCTATTC---------------------------GCCGCGCTCGTCGTCGACAACGGCTC`	`27 bp deleted → no frameshift`
77.4	`CTCGGCTCACAGCGC-----------------------------------------CGCGCTCGTCGTCGACAACGGCTC`	`41 bp deleted → frameshift`
74.1	`CTCGGCTCACAGCGCGCCCGGCTAT----------------------------CGCCGCGCTCGTCGTCGACAACGGCTC`	`28 bp deleted → frameshift`
73.6	`CTCGGCTCACAGCGCGCCCGGCTAT--------------------GATGATATCGCCGCGCTCGTCGTCGACAACGGCTC`	`20 bp deleted → frameshift`
73.2	`CTCGGCTCACAGCGCGCCCGGC----------------------------------CGCGCTCGTCGTCGACAACGGCTC`	`34 bp deleted → frameshift`
73.2	`CTCGGCTCACAGCGCGC------------------------------------------GCTCGTCGTCGACAACGGCTC`	`42 bp deleted → no frameshift`
71.0	`CTCGGCTCACAGCGCGCCCGGCT---------------------------------------CGTCGTCGACAACGGCTC`	`39 bp deleted → no frameshift`
69.6	`CTCGGCTCACAGCGCGCCCG-----------------------------------CCGCGCTCGTCGTCGACAACGGCTC`	`35 bp deleted → frameshift`
67.5	`CTCGGCTCACAGCGCGCCCGGCTATTCTCG----------------------------------------ACAACGGCTC`	`40 bp deleted → frameshift`
66.9	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTC------------------------------GTCGACAACGGCTC`	`30 bp deleted → no frameshift`
63.4	`CTCGGCTCACAGCGCGCCCGGCTAT-----------------------GATATCGCCGCGCTCGTCGTCGACAACGGCTC`	`23 bp deleted → frameshift`
62.8	`CTCGGCTCACAGCGCGCCCGGC-------------------------------------GCTCGTCGTCGACAACGGCTC`	`37 bp deleted → frameshift`
60.0	`CTCGG--------------------------------------ATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTC`	`38 bp deleted → frameshift`
57.6	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCA---------------------------------ACGGCTC`	`33 bp deleted → no frameshift`
57.6	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTC---------------------------------GACAACGGCTC`	`33 bp deleted → no frameshift`
54.9	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCAC----------------------------------AACGGCTC`	`34 bp deleted → frameshift`
54.9	`CTCGGCTCACAGCGCGCCCGGCT--------------------------------------------------------C`	`56 bp deleted → frameshift`
54.6	`CTCGGCTCACAGCGCGCCCGGCTAT--------------------------ATCGCCGCGCTCGTCGTCGACAACGGCTC`	`26 bp deleted → frameshift`
54.0	`CTCGGCTCACAGCGCGCCCG----------------------------------------CTCGTCGTCGACAACGGCTC`	`40 bp deleted → frameshift`
49.5	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCA------------------------------------ACGGCTC`	`36 bp deleted → no frameshift`
49.5	`CTCGGCTCACAGCGCGCCCGGCTATTC------------------------------------GTCGTCGACAACGGCTC`	`36 bp deleted → no frameshift`
47.1	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCAC-------------------------------------GGCTC`	`37 bp deleted → frameshift`
46.4	`CTCGGCTCACAGC-------------------------------------------CGCGCTCGTCGTCGACAACGGCTC`	`43 bp deleted → frameshift`
44.8	`CTCGGCTC-------------------------------------------------------GTCGTCGACAACGGCTC`	`55 bp deleted → frameshift`
44.4	`CTCGGCTCACAGCGCGCCCG--------------------------------------------TCGTCGACAACGGCTC`	`44 bp deleted → frameshift`
42.6	`CTCGGCTCACAGCGCGCCCGGCTATTC---------------------------------------GTCGACAACGGCTC`	`39 bp deleted → no frameshift`
40.0	`CTCGGCTCACAGCGCGCCCGGCTATTCTCG----------------------------------------------GCTC`	`46 bp deleted → frameshift`
39.0	`CTCGGCTCACAGCGCGCCCGGCTATTCTC---------------------------------------------------`	`51 bp deleted → no frameshift`
39.0	`CTCG---------------------------------------------------CCGCGCTCGTCGTCGACAACGGCTC`	`51 bp deleted → no frameshift`
38.7	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGCA-----------------------------------------ACGGCTC`	`41 bp deleted → frameshift`
38.0	`CTCGGCTCACAGCGCGCCCGGCTATTCTCGC-----------------------------------------------TC`	`47 bp deleted → frameshift`
38.0	`CTCGGCTCACAGCGCGCCCG-----------------------------------------------TCGACAACGGCTC`	`47 bp deleted → frameshift`
36.6	`CTCGGCTCACAGCGCGCCCGGCTATTC------------------------------------------GACAACGGCTC`	`42 bp deleted → no frameshift`
36.4	`CTCGGCTCACAGCGCG------------------------------------------------TCGTCGACAACGGCTC`	`48 bp deleted → no frameshift`
36.4	`CTCGGCTCACAGC------------------------------------------------TCGTCGTCGACAACGGCTC`	`48 bp deleted → no frameshift`
35.0	`CTCG------------------------------------------------------------TCGTCGACAACGGCTC`	`60 bp deleted → no frameshift`
32.8	`CTCGGCTCACAGCGCGCCCG--------------------------------------------------ACAACGGCTC`	`50 bp deleted → frameshift`
32.8	`CTCGGCTCACAGCG--------------------------------------------------TCGTCGACAACGGCTC`	`50 bp deleted → frameshift`
32.8	`CTCGGC--------------------------------------------------CGCGCTCGTCGTCGACAACGGCTC`	`50 bp deleted → frameshift`
31.2	`CTCGGCTCACAGCGCG---------------------------------------------------TCGACAACGGCTC`	`51 bp deleted → no frameshift`
30.0	`CTCGGCTC----------------------------------------------GCCGCGCTCGTCGTCGACAACGGCTC`	`46 bp deleted → frameshift`
29.7	`CTCGGCTC------------------------------------------------------------------------`	`72 bp deleted → no frameshift`
28.4	`CTCGGCTCACAGCG-----------------------------------------------------TCGACAACGGCTC`	`53 bp deleted → frameshift`
28.4	`CTCGGC-----------------------------------------------------GCTCGTCGTCGACAACGGCTC`	`53 bp deleted → frameshift`
26.8	`CTCGGCTCACAGCGCG------------------------------------------------------ACAACGGCTC`	`54 bp deleted → no frameshift`
26.8	`CTCG------------------------------------------------------CGCTCGTCGTCGACAACGGCTC`	`54 bp deleted → no frameshift`
24.4	`CTCGGCTCACAGCG--------------------------------------------------------ACAACGGCTC`	`56 bp deleted → frameshift`
24.4	`CTCG--------------------------------------------------------CTCGTCGTCGACAACGGCTC`	`56 bp deleted → frameshift`
21.5	`CTCG---------------------------------------------------------------TCGACAACGGCTC`	`63 bp deleted → no frameshift`
21.3	`CTCGGCTCACAGCGCGCCCGGCTATTC-----------------------------------------------------`	`53 bp deleted → frameshift`
20.0	`CTCGGCTCACAGCGCG------------------------------------------------------------GCTC`	`60 bp deleted → no frameshift`
18.8	`CTCGGCTCACAGCGCGC-------------------------------------------------------------TC`	`61 bp deleted → frameshift`
18.5	`CTCG------------------------------------------------------------------ACAACGGCTC`	`66 bp deleted → no frameshift`
18.0	`CTCGGCTCACAGCG--------------------------------------------------------------GCTC`	`62 bp deleted → frameshift`
18.0	`CTCGGCTCACA--------------------------------------------------------------ACGGCTC`	`62 bp deleted → frameshift`
17.2	`CTCGGCTCACAGCGC---------------------------------------------------------------TC`	`63 bp deleted → no frameshift`
16.5	`CTCGGCTC----------------------------------------------------------GTCGACAACGGCTC`	`58 bp deleted → frameshift`
15.6	`CTCGGCTCACAGC-----------------------------------------------------------------TC`	`65 bp deleted → frameshift`
14.1	`CTCGGCTC-------------------------------------------------------------GACAACGGCTC`	`61 bp deleted → frameshift`
12.3	`CTCGGCTCA----------------------------------------------------------------ACGGCTC`	`64 bp deleted → frameshift`
11.7	`CTCGGCTCAC-----------------------------------------------------------------GGCTC`	`65 bp deleted → frameshift`
10.5	`CTC-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`

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