CRISPOR

Micro-homology scoring of potential deletions

Guide: GGGCGCGCGCCGGCGCCCCC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	(Wild-type sequence)
485.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG----------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`10 bp deleted → frameshift`
417.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGC-------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`13 bp deleted → frameshift`
346.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC-----------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`11 bp deleted → frameshift`
329.4	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGG----------------------------------AAGTGGCCAGGGC`	`34 bp deleted → frameshift`
327.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC--------------------------GCCGGAAGTGGCCAGGGC`	`26 bp deleted → frameshift`
294.4	`GCTTCCTTTGTCCCCAATCTGGGCG--------------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`20 bp deleted → frameshift`
269.4	`GCTTCCTTTGTCCCCAATCTGGGCGCGCG----------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`16 bp deleted → frameshift`
268.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCC--------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`8 bp deleted → frameshift`
268.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC--------GGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`8 bp deleted → frameshift`
256.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCC-----------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`17 bp deleted → frameshift`
255.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC---------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`9 bp deleted → no frameshift`
247.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC----------------------------GGAAGTGGCCAGGGC`	`28 bp deleted → frameshift`
244.2	`GCTTCCTTTGTCCCCAATCTGGGCGCG------------------GCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`18 bp deleted → no frameshift`
242.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC----------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`10 bp deleted → frameshift`
231.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGC------------------------------------CGGAAGTGGCCAGGGC`	`36 bp deleted → no frameshift`
198.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGC--------------GGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`14 bp deleted → frameshift`
190.2	`GCTTCCTTTGTCCCCAATCTGGGC-----------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`23 bp deleted → frameshift`
175.0	`GCTTCCTTTGTCCCCAATCTGG---------------------CGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`21 bp deleted → no frameshift`
161.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGC--------------------------------GCCGGAAGTGGCCAGGGC`	`32 bp deleted → frameshift`
154.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGC-------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`19 bp deleted → frameshift`
147.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGG--------------------ACTCGGCGCGCCGGAAGTGGCCAGGGC`	`20 bp deleted → frameshift`
140.0	`GCTTCCTTTGTCCCCAATCTGGGCGC---------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`21 bp deleted → no frameshift`
126.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG-----------------------GCGCGCCGGAAGTGGCCAGGGC`	`23 bp deleted → frameshift`
120.4	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC------------------------GCGCCGGAAGTGGCCAGGGC`	`24 bp deleted → no frameshift`
120.4	`GCTTCCTTTGTCCCCAATCTGG------------------------CCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`24 bp deleted → no frameshift`
120.0	`GCTTCCTTTGTCCCCAATCTGGGCGC--------------------------------------CGGAAGTGGCCAGGGC`	`38 bp deleted → frameshift`
114.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCC-------------------------GGAAGTGGCCAGGGC`	`25 bp deleted → frameshift`
109.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC--------------------------GGAAGTGGCCAGGGC`	`26 bp deleted → frameshift`
103.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC---------------------------GGAAGTGGCCAGGGC`	`27 bp deleted → no frameshift`
94.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCG-----------------------------CGCCGGAAGTGGCCAGGGC`	`29 bp deleted → frameshift`
94.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCG-----------------------------GCGCGCCGGAAGTGGCCAGGGC`	`29 bp deleted → frameshift`
90.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCC--------------------------------------AGGGC`	`38 bp deleted → frameshift`
89.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGC------------------------------CGGAAGTGGCCAGGGC`	`30 bp deleted → no frameshift`
89.2	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGC------------------------------GCGCCGGAAGTGGCCAGGGC`	`30 bp deleted → no frameshift`
89.2	`GCTTCCTTTGTCCCCAATCTGGG------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGC`	`30 bp deleted → no frameshift`
84.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCG-------------------------------GAAGTGGCCAGGGC`	`31 bp deleted → frameshift`
84.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCG-------------------------------CCGGAAGTGGCCAGGGC`	`31 bp deleted → frameshift`
84.8	`GCTTCCTTTGTCCCCAATCTGGGCGCG-------------------------------GCGCGCCGGAAGTGGCCAGGGC`	`31 bp deleted → frameshift`
84.8	`GCTTCCTTTGTCCCCAATCTGG-------------------------------ACTCGGCGCGCCGGAAGTGGCCAGGGC`	`31 bp deleted → frameshift`
81.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGC----------------------------------------CAGGGC`	`40 bp deleted → frameshift`
76.8	`GCTTCCTTTGTCCCCAATCTGGGCG---------------------------------GCGCGCCGGAAGTGGCCAGGGC`	`33 bp deleted → no frameshift`
76.8	`GCTTCCTTTGTCCCC---------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`33 bp deleted → no frameshift`
73.2	`GCTTCCTTTGTCCC----------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`34 bp deleted → frameshift`
70.5	`GCTTCCTTTGTCCCCAATCT-----------------------------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`29 bp deleted → frameshift`
69.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCC-----------------------------------AGGGC`	`35 bp deleted → frameshift`
69.6	`GCTTCCTTTGTCC-----------------------------------TAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`35 bp deleted → frameshift`
66.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCC--------------------------------------------AGGGC`	`44 bp deleted → frameshift`
66.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCC------------------------------------AGGGC`	`36 bp deleted → no frameshift`
62.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCC-------------------------------------AGGGC`	`37 bp deleted → frameshift`
62.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCG-------------------------------------GAAGTGGCCAGGGC`	`37 bp deleted → frameshift`
62.8	`GCTTCCTTTGTCCCCAATCTGG-------------------------------------CGCGCCGGAAGTGGCCAGGGC`	`37 bp deleted → frameshift`
61.0	`GCTTCCT------------------------------------------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`42 bp deleted → no frameshift`
60.6	`GCTTCCTTTG--------------------------------GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`32 bp deleted → frameshift`
60.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGC----------------------------------------------`	`46 bp deleted → frameshift`
56.8	`GCTTCCTTTGTCCCCAATCTGGGCGCG---------------------------------------GAAGTGGCCAGGGC`	`39 bp deleted → no frameshift`
54.0	`GCTTCCTTTGTCCCCAATCTGGGC----------------------------------------CGGAAGTGGCCAGGGC`	`40 bp deleted → frameshift`
51.6	`GCTTCCTTTGTCCCCAATCTGGGCG-----------------------------------------GAAGTGGCCAGGGC`	`41 bp deleted → frameshift`
49.5	`GCTTCCTTTGTCCCCAATCT------------------------------------CGGCGCGCCGGAAGTGGCCAGGGC`	`36 bp deleted → no frameshift`
49.2	`GCTTCCTTTGTCCCCAATCTGGGC--------------------------------------------------CAGGGC`	`50 bp deleted → frameshift`
48.8	`GCTTCCTTTGTCCCCAATCTGGGC--------------------------------------------------------`	`56 bp deleted → frameshift`
48.8	`GC------------------------------------------GGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`42 bp deleted → no frameshift`
45.0	`GCTTCCTTTGTCCCCAATC--------------------------------------GGCGCGCCGGAAGTGGCCAGGGC`	`38 bp deleted → frameshift`
44.4	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGC--------------------------------------------`	`44 bp deleted → frameshift`
44.4	`GCTTCCTTTGTCCCCAATCTGGG--------------------------------------------AAGTGGCCAGGGC`	`44 bp deleted → frameshift`
42.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGG---------------------------------------------GC`	`45 bp deleted → no frameshift`
42.0	`GCTTCCTTTGTCCCCAATCTGG---------------------------------------------AAGTGGCCAGGGC`	`45 bp deleted → no frameshift`
42.0	`GC---------------------------------------------CTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`45 bp deleted → no frameshift`
40.0	`GCTTCCTTTGTCCCCAATCTGGGCGCGC----------------------------------------------CAGGGC`	`46 bp deleted → frameshift`
39.0	`GCTTCCTTTGTCCCCAATCTGG---------------------------------------------------CCAGGGC`	`51 bp deleted → no frameshift`
36.6	`GCTTCCTTTGTCCCCAA----------------------------------GGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`34 bp deleted → frameshift`
36.4	`GCTTCCTTTGTCCCCAATCTGGGCGC------------------------------------------------CAGGGC`	`48 bp deleted → no frameshift`
32.8	`GCTTCCTTTGTCCCCAATCTGGGCGCGCGC--------------------------------------------------`	`50 bp deleted → frameshift`
32.8	`GCTTCCTTTGTCCCC--------------------------------------------------GGAAGTGGCCAGGGC`	`50 bp deleted → frameshift`
31.5	`GCTTCCTTTGTC---------------------------------------------GGCGCGCCGGAAGTGGCCAGGGC`	`45 bp deleted → no frameshift`
31.2	`GCTTCCTTTGTCCC---------------------------------------------------GGAAGTGGCCAGGGC`	`51 bp deleted → no frameshift`
30.0	`GCT----------------------------------------------AAGGACTCGGCGCGCCGGAAGTGGCCAGGGC`	`46 bp deleted → frameshift`
29.6	`GCTTCCTTTGTCCCCAATCTGGGCGCGC----------------------------------------------------`	`52 bp deleted → frameshift`
29.6	`GCTTCCTTTGTCC----------------------------------------------------GGAAGTGGCCAGGGC`	`52 bp deleted → frameshift`
26.8	`GCTTCCTTTGTCCCCAATCTGGGCGC------------------------------------------------------`	`54 bp deleted → no frameshift`
25.8	`GCTTCCT-------------------------------------------------CGGCGCGCCGGAAGTGGCCAGGGC`	`49 bp deleted → frameshift`
25.6	`GCTTCCTTTGTCCCCAATCTGGG-------------------------------------------------------GC`	`55 bp deleted → frameshift`
25.0	`GCTTCCTTTGTCCCCA------------------------------------------------------------GGGC`	`60 bp deleted → no frameshift`
23.2	`GCTTCCTTTGTCCCCAATCTGG---------------------------------------------------------C`	`57 bp deleted → no frameshift`
22.2	`GCTTC----------------------------------------------------GGCGCGCCGGAAGTGGCCAGGGC`	`52 bp deleted → frameshift`
22.0	`GC----------------------------------------------------------GCGCCGGAAGTGGCCAGGGC`	`58 bp deleted → frameshift`
21.3	`GCT-----------------------------------------------------CGGCGCGCCGGAAGTGGCCAGGGC`	`53 bp deleted → frameshift`
20.8	`GCTTCC-----------------------------------------------------------GGAAGTGGCCAGGGC`	`59 bp deleted → frameshift`
20.0	`GC------------------------------------------------------------GCCGGAAGTGGCCAGGGC`	`60 bp deleted → no frameshift`
18.8	`GCTTCCTTTGTCCC-------------------------------------------------------------AGGGC`	`61 bp deleted → frameshift`
18.0	`GCTTCCTTTGTCC--------------------------------------------------------------AGGGC`	`62 bp deleted → frameshift`
18.0	`GC--------------------------------------------------------------CGGAAGTGGCCAGGGC`	`62 bp deleted → frameshift`
15.0	`GCTTCCTTTGT------------------------------------------------------------GGCCAGGGC`	`60 bp deleted → no frameshift`
14.8	`GCTTCCTTTGTCCCCAA----------------------------------------------------GTGGCCAGGGC`	`52 bp deleted → frameshift`
13.5	`GCTTCCTTTG--------------------------------------------------------------GCCAGGGC`	`62 bp deleted → frameshift`
12.8	`GCTTCC---------------------------------------------------------------------AGGGC`	`69 bp deleted → no frameshift`
10.8	`GC------------------------------------------------------------------------CAGGGC`	`72 bp deleted → no frameshift`
8.0	`GC------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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