Position 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230
Sequence cttcctttgtccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcgcgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggctattctcgcagctcaccatgGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCC
CCT--- CCC--- CCG--- CCC--- CCT--- CCG--- CCA--- CCT--- CCC--- CCA--- CCG--- ---CGG CCG--- ---AGG ---CGG CCC--- CCG--- ---TGG
CCC--- ---CGG CCC--- ---AGG ---CGG ---AGG ---CGG CCG--- ---TGG ---CGG CCG--- CCC--- ---GGG
CCA--- CCC--- ---CGG ---TGG ---CGG ---CGG ---CGG ---GGG CCC--- ---GGG
CCT--- ---GGG CCC---
---TGG ---GGG CCG---
---CGG ---GGG ---CGG
---GGG ---GGG
| Position/ Strand 
|
Guide Sequence + PAM + Restriction Enzymes
Only G- Only GG- Only A-
| Specificity Score
|
Predicted Efficiency
Main scores |
Out-of- Frame
Click score for details |
Off-targets for 0-1-2-3-4 mismatches + next to PAM
|
Genome Browser links to matches sorted by CFD off-target score
No exons. chr7 only |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 204 / rev |
GGAGGGGAAGACGGCCCGGG GGG
High GC content
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
29 | 52 | -- | -- | -- | -- | -- | 101 | -- | -- | None |
0 - 5 - 23 - 37 - 363
0 - 0 - 0 - 1 - 1 428 off-targets |
show all... |
| 205 / rev |
TGGAGGGGAAGACGGCCCGG GGG
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
30 | 52 | -- | -- | -- | -- | -- | 97 | -- | -- | None |
1 - 4 - 12 - 42 - 196
1 - 0 - 0 - 0 - 0 255 off-targets |
show all... |
| 36 / rev |
CCGAGTCCTTAGGCCGCCAG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
87 | 56 | -- | -- | -- | -- | -- | 95 | -- | -- | None |
0 - 0 - 0 - 5 - 49
0 - 0 - 0 - 0 - 0 54 off-targets |
show all... |
| 79 / fw |
CGCGCCGGAAGTGGCCAGGG CGG
High GC content
Enzymes: StyD4I, BseDI, BstNI, BslI
Cloning / PCR primers
|
75 | 43 | -- | -- | -- | -- | -- | 93 | -- | -- | None |
0 - 0 - 0 - 11 - 92
0 - 0 - 0 - 0 - 8 103 off-targets |
show all... |
| 206 / rev |
ATGGAGGGGAAGACGGCCCG GGG
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
29 | 40 | -- | -- | -- | -- | -- | 87 | -- | -- | None |
1 - 4 - 14 - 17 - 122
1 - 0 - 0 - 0 - 0 158 off-targets |
show all... |
| 82 / fw |
GCCGGAAGTGGCCAGGGCGG GGG
High GC content
Enzymes: BslI
Cloning / PCR primers
|
61 | 32 | -- | -- | -- | -- | -- | 86 | -- | -- | None |
0 - 0 - 2 - 36 - 288
0 - 0 - 0 - 2 - 4 326 off-targets |
show all... |
| 13 / rev |
GCGCCGGCGCGCGCCCAGAT TGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
84 | 20 | -- | -- | -- | -- | -- | 78 | -- | -- | None |
0 - 0 - 0 - 15 - 219
0 - 0 - 0 - 0 - 0 234 off-targets |
show all... |
| 210 / fw |
CGCGGGCGACGATGCCCCCC GGG
High GC content
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
16 | 40 | -- | -- | -- | -- | -- | 78 | -- | -- | None |
0 - 5 - 1 - 8 - 74
0 - 5 - 0 - 4 - 2 88 off-targets |
show all... |
| 43 / fw |
CGCGCGCCGGCGCCCCCTGG CGG
High GC content
Enzymes: BshFI, TauI, BseDI, BstNI, BslI, StyD4I, Fsp4HI
Cloning / PCR primers
|
41 | 47 | -- | -- | -- | -- | -- | 77 | -- | -- | None |
0 - 0 - 12 - 31 - 255
0 - 0 - 5 - 6 - 30 298 off-targets |
|