Homo sapiens (hg38), chr7:5529546-5529784, reverse genomic strand
Found 52 possible guide sequences in input (239 bp). Click on a PAM NGG match to show its 20 bp guide sequence.
Shown below are the PAM site and the expected cleavage position located -3bp 5' of the PAM site.
Colors green, yellow and red indicate high, medium and low specificity of the PAM's guide sequence in the genome.

Suggest a genome variants database to show on this page

Position  0         10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       
Sequence  cttcctttgtccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcgcgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggctattctcgcagctcaccatgGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCC
             CCT---  CCC---           CCG---  CCC---   CCT---           CCG---    CCA---         CCT---           CCC---               CCA---            CCG---          ---CGG  CCG---   ---AGG      ---CGG         CCC---   CCG---         ---TGG
                    CCC---          ---CGG  CCC---      ---AGG        ---CGG     ---AGG          ---CGG            CCG---              ---TGG                                  ---CGG           CCG---                CCC---                  ---GGG
                      CCA---                 CCC---           ---CGG        ---TGG   ---CGG                      ---CGG                                                                       ---CGG   ---GGG          CCC---                  ---GGG
                                               CCT---                             ---GGG                                                                                                                                CCC---
                                              ---TGG                                  ---GGG                                                                                                                             CCG---
                                                 ---CGG                                ---GGG                                                                                                                          ---CGG
                                                                                        ---GGG                                                                                                                          ---GGG

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Predicted guide sequences for PAMs
Ranked by default from highest to lowest specificity score (Hsu et al., Nat Biot 2013). Click on a column title to rank by a score.
If you use this website, please cite our CRISPOR paper in Gen Biol 2016. Too much information? Look at the CRISPOR manual.

Download as Excel tables: Guides /  Off-targets /  Saturating mutagenesis assistant
Position/
Strand
Guide Sequence + PAM
+ Restriction Enzymes
Only G- Only GG- Only A-
Specificity Score Predicted Efficiency
Show all scores
Out-of-Frame score

Click on score to show micro-homology
Off-targets for
0-1-2-3-4 mismatches
+ next to PAM
Genome Browser links to matches sorted by CFD off-target score
No exons. chr7 only
204 / rev GGAGGGGAAGACGGCCCGGG GGG
High GC content
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, BglI, NciI, StyD4I, Cfr9I
 Cloning / PCR primers
29 52 101 None 0 - 5 - 23 - 37 - 363
0 - 0 - 0 - 1 - 1

428 off-targets
show all...
205 / rev TGGAGGGGAAGACGGCCCGG GGG
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
 Cloning / PCR primers
30 52 97 None 1 - 4 - 12 - 42 - 196
1 - 0 - 0 - 0 - 0

255 off-targets
show all...
36 / rev CCGAGTCCTTAGGCCGCCAG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
 Cloning / PCR primers
87 56 95 None 0 - 0 - 0 - 5 - 49
0 - 0 - 0 - 0 - 0

54 off-targets
show all...
79 / fw CGCGCCGGAAGTGGCCAGGG CGG
High GC content
Enzymes: StyD4I, BseDI, BstNI, BslI
 Cloning / PCR primers
75 43 93 None 0 - 0 - 0 - 11 - 92
0 - 0 - 0 - 0 - 8

103 off-targets
show all...
206 / rev ATGGAGGGGAAGACGGCCCG GGG
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
 Cloning / PCR primers
29 40 87 None 1 - 4 - 14 - 17 - 122
1 - 0 - 0 - 0 - 0

158 off-targets
show all...
82 / fw GCCGGAAGTGGCCAGGGCGG GGG
High GC content
Enzymes: BslI
 Cloning / PCR primers
61 32 86 None 0 - 0 - 2 - 36 - 288
0 - 0 - 0 - 2 - 4

326 off-targets
show all...
13 / rev GCGCCGGCGCGCGCCCAGAT TGG
High GC content
Enzymes: BslFI, BslI
 Cloning / PCR primers
84 20 78 None 0 - 0 - 0 - 15 - 219
0 - 0 - 0 - 0 - 0

234 off-targets
show all...
210 / fw CGCGGGCGACGATGCCCCCC GGG
High GC content
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
 Cloning / PCR primers
16 40 78 None 0 - 5 - 1 - 8 - 74
0 - 5 - 0 - 4 - 2

88 off-targets
show all...
43 / fw CGCGCGCCGGCGCCCCCTGG CGG
High GC content
Enzymes: BshFI, TauI, BseDI, BstNI, BslI, StyD4I, Fsp4HI
 Cloning / PCR primers
41 47 77 None 0 - 0 - 12 - 31 - 255
0 - 0 - 5 - 6 - 30

298 off-targets
show all...
207 / rev GATGGAGGGGAAGACGGCCC GGG
Enzymes: BshFI, BmeT110I, MwoI, NciI, MspI, BseDI, PspPI, BglI, BceAI, StyD4I, Cfr9I
 Cloning / PCR primers
21 33 77 None 1 - 4 - 16 - 32 - 265
1 - 2 - 0 - 1 - 0

318 off-targets
show all...
4 / rev CGCGCCCAGATTGGGGACAA AGG
Enzymes: BslFI
 Cloning / PCR primers
85 51 76 None 0 - 0 - 1 - 4 - 65
0 - 0 - 0 - 1 - 0

70 off-targets
show all...
91 / fw GGCCAGGGCGGGGGCGACCT CGG
High GC content
Enzymes: BseDI, MwoI, MnlI
 Cloning / PCR primers
60 42 71 None 0 - 0 - 3 - 29 - 324
0 - 0 - 0 - 0 - 3

356 off-targets
show all...
50 / fw CGGCGCCCCCTGGCGGCCTA AGG
High GC content
Enzymes: BshFI, BstDEI, Bse21I, HinfI
 Cloning / PCR primers
77 29 68 None 0 - 0 - 3 - 23 - 136
0 - 0 - 0 - 1 - 0

162 off-targets
show all...
209 / fw TCGCGGGCGACGATGCCCCC CGG
High GC content
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
 Cloning / PCR primers
18 36 68 None 0 - 5 - 4 - 12 - 26
0 - 5 - 0 - 4 - 1

47 off-targets
show all...
80 / fw GCGCCGGAAGTGGCCAGGGC GGG
High GC content
Enzymes: StyD4I, BseDI, BstNI, BslI
 Cloning / PCR primers
53 28 67 None 0 - 0 - 3 - 26 - 214
0 - 0 - 0 - 6 - 14

243 off-targets
show all...
35 / rev CGAGTCCTTAGGCCGCCAGG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
 Cloning / PCR primers
82 65 64 None 0 - 0 - 3 - 7 - 65
0 - 0 - 0 - 0 - 1

75 off-targets
show all...
163 / fw GCCGCGCTCGTCGTCGACAA CGG
Enzymes: NlaIV, BceAI, Hpy166II, HincII, LmnI, SalI, FblI
 Cloning / PCR primers
91 57 64 None 0 - 0 - 0 - 14 - 14
0 - 0 - 0 - 1 - 0

28 off-targets
show all...
70 / fw AGGACTCGGCGCGCCGGAAG TGG
Enzymes: BshFI, StyD4I, BalI, AcoI, BstNI
 Cloning / PCR primers
93 47 61 None 0 - 0 - 0 - 6 - 46
0 - 0 - 0 - 0 - 1

52 off-targets
show all...
40 / fw GGGCGCGCGCCGGCGCCCCC TGG
High GC content
Enzymes: BshFI, TauI, BseDI, BstNI, BslI, StyD4I, Fsp4HI
 Cloning / PCR primers
44 34 60 None 0 - 0 - 12 - 58 - 361
0 - 0 - 1 - 7 - 2

431 off-targets
show all...
56 / fw CCCCTGGCGGCCTAAGGACT CGG
Enzymes: HinP1I, BspFNI, HinfI, BssHII, BstC8I
 Cloning / PCR primers
84 33 60 None 0 - 0 - 0 - 8 - 100
0 - 0 - 0 - 0 - 4

108 off-targets
show all...
88 / rev GCCGGGCGCGCTGTGAGCCG AGG
High GC content
Enzymes: NmeAIII, BseDI, MwoI
 Cloning / PCR primers
74 57 60 None 0 - 0 - 2 - 22 - 178
0 - 0 - 1 - 1 - 1

202 off-targets
show all...
233 / fw CCGTCTTCCCCTCCATCGTG GGG
Enzymes: BslI
 Cloning / PCR primers
20 64 60 None 1 - 4 - 12 - 14 - 103
1 - 1 - 0 - 0 - 1

134 off-targets
show all...
144 / rev GCCGTTGTCGACGACGAGCG CGG
Enzymes: HinP1I, BspFNI, TauI, PcsI, Fsp4HI
 Cloning / PCR primers
86 62 59 None 0 - 1 - 1 - 9 - 10
0 - 0 - 0 - 0 - 1

21 off-targets
show all...
169 / fw CTCGTCGTCGACAACGGCTC CGG
Enzymes: NlaIV, MspI, BceAI, NlaIII, LmnI, XceI
 Cloning / PCR primers
43 52 59 None 0 - 1 - 13 - 4 - 13
0 - 1 - 0 - 0 - 2

31 off-targets
show all...
12 / rev CGCCGGCGCGCGCCCAGATT GGG
High GC content
Enzymes: BslFI, BslI
 Cloning / PCR primers
96 22 58 None 0 - 0 - 0 - 3 - 28
0 - 0 - 0 - 0 - 0

31 off-targets
show all...
46 / rev TTCCGGCGCGCCGAGTCCTT AGG
Enzymes: BshFI, Bse21I, TauI, BstDEI, SsiI, Fsp4HI
 Cloning / PCR primers
97 47 57 None 0 - 0 - 0 - 0 - 14
0 - 0 - 0 - 0 - 1

14 off-targets
show all...
81 / fw CGCCGGAAGTGGCCAGGGCG GGG
High GC content
Enzymes: BseDI, BslI
 Cloning / PCR primers
62 23 57 None 0 - 0 - 2 - 18 - 153
0 - 0 - 0 - 1 - 6

173 off-targets
show all...
37 / rev GCCGAGTCCTTAGGCCGCCA GGG
Enzymes: TauI, HinP1I, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
 Cloning / PCR primers
90 47 56 None 0 - 0 - 0 - 9 - 38
0 - 0 - 0 - 0 - 0

47 off-targets
show all...
29 / rev CTTAGGCCGCCAGGGGGCGC CGG
High GC content
Enzymes: NlaIV, AcyI, BfoI, MspI, BssHII, Cfr10I, HinP1I, BanI, SgrAI, SspDI, NaeI, BspFNI, MreI, BstC8I
 Cloning / PCR primers
63 34 55 None 0 - 0 - 2 - 22 - 125
0 - 0 - 1 - 3 - 7

149 off-targets
show all...
106 / rev TGGTGAGCTGCGAGAATAGC CGG
Enzymes: MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI
 Cloning / PCR primers
78 43 54 None 0 - 0 - 1 - 9 - 109
0 - 0 - 0 - 0 - 1

119 off-targets
show all...
107 / fw ACCTCGGCTCACAGCGCGCC CGG
Enzymes: BssHII, MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI, BstC8I
 Cloning / PCR primers
91 43 54 None 0 - 0 - 0 - 8 - 73
0 - 0 - 0 - 1 - 0

81 off-targets
show all...
193 / fw ATGTGCAAGGCCGGCTTCGC GGG
Enzymes: BspFNI, Hpy99I, MwoI, BstC8I
 Cloning / PCR primers
23 41 54 None 1 - 10 - 8 - 14 - 82
1 - 0 - 0 - 0 - 0

115 off-targets
show all...
11 / rev GCCGGCGCGCGCCCAGATTG GGG
High GC content
Enzymes: BslFI, BslI
 Cloning / PCR primers
77 44 53 None 0 - 1 - 0 - 7 - 73
0 - 0 - 0 - 0 - 0

81 off-targets
show all...
75 / fw TCGGCGCGCCGGAAGTGGCC AGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, StyD4I
 Cloning / PCR primers
90 40 52 None 0 - 0 - 1 - 4 - 28
0 - 0 - 1 - 2 - 1

33 off-targets
show all...
168 / rev GAAGCCGGCCTTGCACATGC CGG
Enzymes: MspI, NlaIII, NlaIV, XceI
 Cloning / PCR primers
21 53 49 None 2 - 11 - 7 - 18 - 99
2 - 11 - 2 - 1 - 1

137 off-targets
show all...
76 / fw CGGCGCGCCGGAAGTGGCCA GGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, BslI, StyD4I
 Cloning / PCR primers
87 52 47 None 0 - 0 - 1 - 5 - 52
0 - 0 - 0 - 0 - 2

58 off-targets
show all...
208 / rev CGATGGAGGGGAAGACGGCC CGG
Enzymes: BshFI, BmeT110I, MwoI, NciI, MspI, BseDI, PspPI, BglI, BceAI, StyD4I, Cfr9I
 Cloning / PCR primers
24 37 47 None 1 - 4 - 12 - 21 - 165
1 - 2 - 1 - 0 - 4

203 off-targets
show all...
105 / rev GGTGAGCTGCGAGAATAGCC GGG
Enzymes: BssHII, MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI, BstC8I
 Cloning / PCR primers
73 59 46 None 0 - 0 - 4 - 29 - 120
0 - 0 - 0 - 0 - 0

153 off-targets
show all...
192 / fw CATGTGCAAGGCCGGCTTCG CGG
Enzymes: BspFNI, MwoI, BstC8I
 Cloning / PCR primers
25 61 46 None 1 - 10 - 6 - 5 - 75
1 - 0 - 0 - 0 - 0

97 off-targets
show all...
213 / rev CCCCACGATGGAGGGGAAGA CGG
Enzymes: BshFI, MboII, MspI, PspPI, BbsI, BceAI, NciI, StyD4I
 Cloning / PCR primers
17 50 46 None 2 - 10 - 14 - 55 - 250
2 - 1 - 4 - 22 - 13

331 off-targets
show all...
64 / fw GGCCTAAGGACTCGGCGCGC CGG
Enzymes: BssHII, MspI, PalAI, HinP1I, BspFNI, BstC8I
 Cloning / PCR primers
92 51 45 None 0 - 0 - 0 - 1 - 50
0 - 0 - 0 - 0 - 0

51 off-targets
show all...
183 / rev GGCATCGTCGCCCGCGAAGC CGG
Enzymes: BshFI, MwoI, MspI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
41 39 43 None 0 - 6 - 5 - 6 - 30
0 - 0 - 0 - 0 - 2

47 off-targets
show all...
73 / rev AGCCGAGGTCGCCCCCGCCC TGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, BslI, FauI, StyD4I, SsiI
 Cloning / PCR primers
69 43 42 None 0 - 0 - 1 - 16 - 181
0 - 0 - 0 - 1 - 3

198 off-targets
show all...
184 / fw GGCTCCGGCATGTGCAAGGC CGG
Enzymes: BshFI, MwoI, MspI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
11 47 42 None 1 - 14 - 11 - 23 - 144
1 - 12 - 2 - 3 - 2

193 off-targets
show all...
231 / fw GGCCGTCTTCCCCTCCATCG TGG
Enzymes: HinP1I, BccI, BslI
 Cloning / PCR primers
23 53 41 None 1 - 3 - 14 - 17 - 102
1 - 1 - 0 - 1 - 3

137 off-targets
show all...
38 / rev CGCCGAGTCCTTAGGCCGCC AGG
Enzymes: TauI, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
 Cloning / PCR primers
91 31 40 None 0 - 0 - 0 - 2 - 53
0 - 0 - 0 - 0 - 0

55 off-targets
show all...
126 / rev CGCGGCGATATCATCATCCA TGG
Enzymes: AsuHPI, BstDSI, BseDI, ErhI, NlaIII, Bsp19I
 Cloning / PCR primers
32 55 39 None 0 - 2 - 6 - 8 - 21
0 - 2 - 4 - 8 - 4

37 off-targets
show all...
30 / fw TCCCCAATCTGGGCGCGCGC CGG
Enzymes: NlaIV, AcyI, BfoI, MspI, BssHII, Cfr10I, HinP1I, BanI, SgrAI, SspDI, NaeI, BspFNI, MreI, BstC8I
 Cloning / PCR primers
93 40 36 None 0 - 0 - 0 - 0 - 48
0 - 0 - 0 - 0 - 1

48 off-targets
show all...
63 / rev GCCCCCGCCCTGGCCACTTC CGG
High GC content
Enzymes: MspI, HinP1I, BssHII, BspFNI, BstC8I
 Cloning / PCR primers
63 30 36 None 0 - 0 - 3 - 27 - 274
0 - 0 - 0 - 6 - 6

304 off-targets
show all...
180 / fw CAACGGCTCCGGCATGTGCA AGG
Enzymes: BshFI, MwoI, HpyCH4V, MspI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
7 56 35 None 1 - 14 - 11 - 14 - 52
1 - 3 - 0 - 0 - 0

92 off-targets
show all...
232 / fw GCCGTCTTCCCCTCCATCGT GGG
Enzymes: BccI
 Cloning / PCR primers
18 51 35 None 1 - 4 - 17 - 17 - 87
1 - 2 - 0 - 0 - 0

126 off-targets
show all...
129 / fw GCTATTCTCGCAGCTCACCA TGG
Enzymes: BstDSI, BtsCI, BseDI, ErhI, NlaIII, Bsp19I
 Cloning / PCR primers
79 55 34 None 0 - 0 - 1 - 16 - 63
0 - 0 - 0 - 1 - 3

80 off-targets
show all...
Download as Excel tables: Guides /  Off-targets /  Saturating mutagenesis assistant
Tab-sep format: Guides /  Off-targets

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