Position 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230
Sequence cttcctttgtccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcgcgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggctattctcgcagctcaccatgGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCC
CCT--- CCC--- CCG--- CCC--- CCT--- CCG--- CCA--- CCT--- CCC--- CCA--- CCG--- ---CGG CCG--- ---AGG ---CGG CCC--- CCG--- ---TGG
CCC--- ---CGG CCC--- ---AGG ---CGG ---AGG ---CGG CCG--- ---TGG ---CGG CCG--- CCC--- ---GGG
CCA--- CCC--- ---CGG ---TGG ---CGG ---CGG ---CGG ---GGG CCC--- ---GGG
CCT--- ---GGG CCC---
---TGG ---GGG CCG---
---CGG ---GGG ---CGG
---GGG ---GGG
| Position/ Strand 
|
Guide Sequence + PAM + Restriction Enzymes
Only G- Only GG- Only A-
| Specificity Score
|
Predicted Efficiency
Main scores |
Out-of- Frame
Click score for details |
Off-targets for 0-1-2-3-4 mismatches + next to PAM
|
Genome Browser links to matches sorted by CFD off-target score
No exons. chr7 only |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4 / rev |
CGCGCCCAGATTGGGGACAA AGG
Enzymes: BslFI
Cloning / PCR primers
|
85 | 51 | -- | -- | -- | -- | -- | 76 | -- | -- | None |
0 - 0 - 1 - 4 - 65
0 - 0 - 0 - 1 - 0 70 off-targets |
show all... |
| 11 / rev |
GCCGGCGCGCGCCCAGATTG GGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
77 | 44 | -- | -- | -- | -- | -- | 53 | -- | -- | None |
0 - 1 - 0 - 7 - 73
0 - 0 - 0 - 0 - 0 81 off-targets |
show all... |
| 12 / rev |
CGCCGGCGCGCGCCCAGATT GGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
96 | 22 | -- | -- | -- | -- | -- | 58 | -- | -- | None |
0 - 0 - 0 - 3 - 28
0 - 0 - 0 - 0 - 0 31 off-targets |
show all... |
| 13 / rev |
GCGCCGGCGCGCGCCCAGAT TGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
84 | 20 | -- | -- | -- | -- | -- | 78 | -- | -- | None |
0 - 0 - 0 - 15 - 219
0 - 0 - 0 - 0 - 0 234 off-targets |
show all... |
| 29 / rev |
CTTAGGCCGCCAGGGGGCGC CGG
High GC content
Enzymes: NlaIV, AcyI, BfoI, MspI, BssHII, Cfr10I, HinP1I, BanI, SgrAI, SspDI, NaeI, BspFNI, MreI, BstC8I
Cloning / PCR primers
|
63 | 34 | -- | -- | -- | -- | -- | 55 | -- | -- | None |
0 - 0 - 2 - 22 - 125
0 - 0 - 1 - 3 - 7 149 off-targets |
show all... |
| 30 / fw |
TCCCCAATCTGGGCGCGCGC CGG
Enzymes: NlaIV, AcyI, BfoI, MspI, BssHII, Cfr10I, HinP1I, BanI, SgrAI, SspDI, NaeI, BspFNI, MreI, BstC8I
Cloning / PCR primers
|
93 | 40 | -- | -- | -- | -- | -- | 36 | -- | -- | None |
0 - 0 - 0 - 0 - 48
0 - 0 - 0 - 0 - 1 48 off-targets |
show all... |
| 35 / rev |
CGAGTCCTTAGGCCGCCAGG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
82 | 65 | -- | -- | -- | -- | -- | 64 | -- | -- | None |
0 - 0 - 3 - 7 - 65
0 - 0 - 0 - 0 - 1 75 off-targets |
show all... |
| 36 / rev |
CCGAGTCCTTAGGCCGCCAG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
87 | 56 | -- | -- | -- | -- | -- | 95 | -- | -- | None |
0 - 0 - 0 - 5 - 49
0 - 0 - 0 - 0 - 0 54 off-targets |
show all... |
| 37 / rev |
GCCGAGTCCTTAGGCCGCCA GGG
Enzymes: TauI, HinP1I, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
Cloning / PCR primers
|
90 | 47 | -- | -- | -- | -- | -- | 56 | -- | -- | None |
0 - 0 - 0 - 9 - 38
0 - 0 - 0 - 0 - 0 47 off-targets |
show all... |
| 38 / rev |
CGCCGAGTCCTTAGGCCGCC AGG
Enzymes: TauI, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
Cloning / PCR primers
|
91 | 31 | -- | -- | -- | -- | -- | 40 | -- | -- | None |
0 - 0 - 0 - 2 - 53
0 - 0 - 0 - 0 - 0 55 off-targets |
show all... |
| 40 / fw |
GGGCGCGCGCCGGCGCCCCC TGG
High GC content
Enzymes: BshFI, TauI, BseDI, BstNI, BslI, StyD4I, Fsp4HI
Cloning / PCR primers
|
44 | 34 | -- | -- | -- | -- | -- | 60 | -- | -- | None |
0 - 0 - 12 - 58 - 361
0 - 0 - 1 - 7 - 2 431 off-targets |
show all... |
| 43 / fw |
CGCGCGCCGGCGCCCCCTGG CGG
High GC content
Enzymes: BshFI, TauI, BseDI, BstNI, BslI, StyD4I, Fsp4HI
Cloning / PCR primers
|
41 | 47 | -- | -- | -- | -- | -- | 77 | -- | -- | None |
0 - 0 - 12 - 31 - 255
0 - 0 - 5 - 6 - 30 298 off-targets |
show all... |
| 46 / rev |
TTCCGGCGCGCCGAGTCCTT AGG
Enzymes: BshFI, Bse21I, TauI, BstDEI, SsiI, Fsp4HI
Cloning / PCR primers
|
97 | 47 | -- | -- | -- | -- | -- | 57 | -- | -- | None |
0 - 0 - 0 - 0 - 14
0 - 0 - 0 - 0 - 1 14 off-targets |
show all... |
| 50 / fw |
CGGCGCCCCCTGGCGGCCTA AGG
High GC content
Enzymes: BshFI, BstDEI, Bse21I, HinfI
Cloning / PCR primers
|
77 | 29 | -- | -- | -- | -- | -- | 68 | -- | -- | None |
0 - 0 - 3 - 23 - 136
0 - 0 - 0 - 1 - 0 162 off-targets |
show all... |
| 56 / fw |
CCCCTGGCGGCCTAAGGACT CGG
Enzymes: HinP1I, BspFNI, HinfI, BssHII, BstC8I
Cloning / PCR primers
|
84 | 33 | -- | -- | -- | -- | -- | 60 | -- | -- | None |
0 - 0 - 0 - 8 - 100
0 - 0 - 0 - 0 - 4 108 off-targets |
|