Position 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230
Sequence cttcctttgtccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcgcgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggctattctcgcagctcaccatgGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCC
CCT--- CCC--- CCG--- CCC--- CCT--- CCG--- CCA--- CCT--- CCC--- CCA--- CCG--- ---CGG CCG--- ---AGG ---CGG CCC--- CCG--- ---TGG
CCC--- ---CGG CCC--- ---AGG ---CGG ---AGG ---CGG CCG--- ---TGG ---CGG CCG--- CCC--- ---GGG
CCA--- CCC--- ---CGG ---TGG ---CGG ---CGG ---CGG ---GGG CCC--- ---GGG
CCT--- ---GGG CCC---
---TGG ---GGG CCG---
---CGG ---GGG ---CGG
---GGG ---GGG
| Position/ Strand 
|
Guide Sequence + PAM + Restriction Enzymes
Only G- Only GG- Only A-
| Specificity Score
|
Predicted Efficiency
Main scores |
Out-of- Frame
Click score for details |
Off-targets for 0-1-2-3-4 mismatches + next to PAM
|
Genome Browser links to matches sorted by CFD off-target score
No exons. chr7 only |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 46 / rev |
TTCCGGCGCGCCGAGTCCTT AGG
Enzymes: BshFI, Bse21I, TauI, BstDEI, SsiI, Fsp4HI
Cloning / PCR primers
|
97 | 47 | -- | -- | -- | -- | -- | 57 | -- | -- | None |
0 - 0 - 0 - 0 - 14
0 - 0 - 0 - 0 - 1 14 off-targets |
show all... |
| 144 / rev |
GCCGTTGTCGACGACGAGCG CGG
Enzymes: HinP1I, BspFNI, TauI, PcsI, Fsp4HI
Cloning / PCR primers
|
86 | 62 | -- | -- | -- | -- | -- | 59 | -- | -- | None |
0 - 1 - 1 - 9 - 10
0 - 0 - 0 - 0 - 1 21 off-targets |
show all... |
| 163 / fw |
GCCGCGCTCGTCGTCGACAA CGG
Enzymes: NlaIV, BceAI, Hpy166II, HincII, LmnI, SalI, FblI
Cloning / PCR primers
|
91 | 57 | -- | -- | -- | -- | -- | 64 | -- | -- | None |
0 - 0 - 0 - 14 - 14
0 - 0 - 0 - 1 - 0 28 off-targets |
show all... |
| 12 / rev |
CGCCGGCGCGCGCCCAGATT GGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
96 | 22 | -- | -- | -- | -- | -- | 58 | -- | -- | None |
0 - 0 - 0 - 3 - 28
0 - 0 - 0 - 0 - 0 31 off-targets |
show all... |
| 169 / fw |
CTCGTCGTCGACAACGGCTC CGG
Enzymes: NlaIV, MspI, BceAI, NlaIII, LmnI, XceI
Cloning / PCR primers
|
43 | 52 | -- | -- | -- | -- | -- | 59 | -- | -- | None |
0 - 1 - 13 - 4 - 13
0 - 1 - 0 - 0 - 2 31 off-targets |
show all... |
| 75 / fw |
TCGGCGCGCCGGAAGTGGCC AGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, StyD4I
Cloning / PCR primers
|
90 | 40 | -- | -- | -- | -- | -- | 52 | -- | -- | None |
0 - 0 - 1 - 4 - 28
0 - 0 - 1 - 2 - 1 33 off-targets |
show all... |
| 126 / rev |
CGCGGCGATATCATCATCCA TGG
Enzymes: AsuHPI, BstDSI, BseDI, ErhI, NlaIII, Bsp19I
Cloning / PCR primers
|
32 | 55 | -- | -- | -- | -- | -- | 39 | -- | -- | None |
0 - 2 - 6 - 8 - 21
0 - 2 - 4 - 8 - 4 37 off-targets |
show all... |
| 37 / rev |
GCCGAGTCCTTAGGCCGCCA GGG
Enzymes: TauI, HinP1I, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
Cloning / PCR primers
|
90 | 47 | -- | -- | -- | -- | -- | 56 | -- | -- | None |
0 - 0 - 0 - 9 - 38
0 - 0 - 0 - 0 - 0 47 off-targets |
show all... |
| 183 / rev |
GGCATCGTCGCCCGCGAAGC CGG
Enzymes: BshFI, MwoI, MspI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
41 | 39 | -- | -- | -- | -- | -- | 43 | -- | -- | None |
0 - 6 - 5 - 6 - 30
0 - 0 - 0 - 0 - 2 47 off-targets |
show all... |
| 209 / fw |
TCGCGGGCGACGATGCCCCC CGG
High GC content
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
18 | 36 | -- | -- | -- | -- | -- | 68 | -- | -- | None |
0 - 5 - 4 - 12 - 26
0 - 5 - 0 - 4 - 1 47 off-targets |
show all... |
| 30 / fw |
TCCCCAATCTGGGCGCGCGC CGG
Enzymes: NlaIV, AcyI, BfoI, MspI, BssHII, Cfr10I, HinP1I, BanI, SgrAI, SspDI, NaeI, BspFNI, MreI, BstC8I
Cloning / PCR primers
|
93 | 40 | -- | -- | -- | -- | -- | 36 | -- | -- | None |
0 - 0 - 0 - 0 - 48
0 - 0 - 0 - 0 - 1 48 off-targets |
show all... |
| 64 / fw |
GGCCTAAGGACTCGGCGCGC CGG
Enzymes: BssHII, MspI, PalAI, HinP1I, BspFNI, BstC8I
Cloning / PCR primers
|
92 | 51 | -- | -- | -- | -- | -- | 45 | -- | -- | None |
0 - 0 - 0 - 1 - 50
0 - 0 - 0 - 0 - 0 51 off-targets |
show all... |
| 70 / fw |
AGGACTCGGCGCGCCGGAAG TGG
Enzymes: BshFI, StyD4I, BalI, AcoI, BstNI
Cloning / PCR primers
|
93 | 47 | -- | -- | -- | -- | -- | 61 | -- | -- | None |
0 - 0 - 0 - 6 - 46
0 - 0 - 0 - 0 - 1 52 off-targets |
show all... |
| 36 / rev |
CCGAGTCCTTAGGCCGCCAG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
87 | 56 | -- | -- | -- | -- | -- | 95 | -- | -- | None |
0 - 0 - 0 - 5 - 49
0 - 0 - 0 - 0 - 0 54 off-targets |
show all... |
| 38 / rev |
CGCCGAGTCCTTAGGCCGCC AGG
Enzymes: TauI, BseDI, BstNI, BslI, StyD4I, SsiI, Fsp4HI
Cloning / PCR primers
|
91 | 31 | -- | -- | -- | -- | -- | 40 | -- | -- | None |
0 - 0 - 0 - 2 - 53
0 - 0 - 0 - 0 - 0 55 off-targets |
show all... |
| 76 / fw |
CGGCGCGCCGGAAGTGGCCA GGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, BslI, StyD4I
Cloning / PCR primers
|
87 | 52 | -- | -- | -- | -- | -- | 47 | -- | -- | None |
0 - 0 - 1 - 5 - 52
0 - 0 - 0 - 0 - 2 58 off-targets |
show all... |
| 4 / rev |
CGCGCCCAGATTGGGGACAA AGG
Enzymes: BslFI
Cloning / PCR primers
|
85 | 51 | -- | -- | -- | -- | -- | 76 | -- | -- | None |
0 - 0 - 1 - 4 - 65
0 - 0 - 0 - 1 - 0 70 off-targets |
show all... |
| 35 / rev |
CGAGTCCTTAGGCCGCCAGG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
82 | 65 | -- | -- | -- | -- | -- | 64 | -- | -- | None |
0 - 0 - 3 - 7 - 65
0 - 0 - 0 - 0 - 1 75 off-targets |
show all... |
| 129 / fw |
GCTATTCTCGCAGCTCACCA TGG
Enzymes: BstDSI, BtsCI, BseDI, ErhI, NlaIII, Bsp19I
Cloning / PCR primers
|
79 | 55 | -- | -- | -- | -- | -- | 34 | -- | -- | None |
0 - 0 - 1 - 16 - 63
0 - 0 - 0 - 1 - 3 80 off-targets |
show all... |
| 11 / rev |
GCCGGCGCGCGCCCAGATTG GGG
High GC content
Enzymes: BslFI, BslI
Cloning / PCR primers
|
77 | 44 | -- | -- | -- | -- | -- | 53 | -- | -- | None |
0 - 1 - 0 - 7 - 73
0 - 0 - 0 - 0 - 0 81 off-targets |
show all... |
| 107 / fw |
ACCTCGGCTCACAGCGCGCC CGG
Enzymes: BssHII, MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI, BstC8I
Cloning / PCR primers
|
91 | 43 | -- | -- | -- | -- | -- | 54 | -- | -- | None |
0 - 0 - 0 - 8 - 73
0 - 0 - 0 - 1 - 0 81 off-targets |
show all... |
| 210 / fw |
CGCGGGCGACGATGCCCCCC GGG
High GC content
Enzymes: BshFI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
16 | 40 | -- | -- | -- | -- | -- | 78 | -- | -- | None |
0 - 5 - 1 - 8 - 74
0 - 5 - 0 - 4 - 2 88 off-targets |
show all... |
| 180 / fw |
CAACGGCTCCGGCATGTGCA AGG
Enzymes: BshFI, MwoI, HpyCH4V, MspI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
7 | 56 | -- | -- | -- | -- | -- | 35 | -- | -- | None |
1 - 14 - 11 - 14 - 52
1 - 3 - 0 - 0 - 0 92 off-targets |
show all... |
| 192 / fw |
CATGTGCAAGGCCGGCTTCG CGG
Enzymes: BspFNI, MwoI, BstC8I
Cloning / PCR primers
|
25 | 61 | -- | -- | -- | -- | -- | 46 | -- | -- | None |
1 - 10 - 6 - 5 - 75
1 - 0 - 0 - 0 - 0 97 off-targets |
show all... |
| 79 / fw |
CGCGCCGGAAGTGGCCAGGG CGG
High GC content
Enzymes: StyD4I, BseDI, BstNI, BslI
Cloning / PCR primers
|
75 | 43 | -- | -- | -- | -- | -- | 93 | -- | -- | None |
0 - 0 - 0 - 11 - 92
0 - 0 - 0 - 0 - 8 103 off-targets |
show all... |
| 56 / fw |
CCCCTGGCGGCCTAAGGACT CGG
Enzymes: HinP1I, BspFNI, HinfI, BssHII, BstC8I
Cloning / PCR primers
|
84 | 33 | -- | -- | -- | -- | -- | 60 | -- | -- | None |
0 - 0 - 0 - 8 - 100
0 - 0 - 0 - 0 - 4 108 off-targets |
show all... |
| 193 / fw |
ATGTGCAAGGCCGGCTTCGC GGG
Enzymes: BspFNI, Hpy99I, MwoI, BstC8I
Cloning / PCR primers
|
23 | 41 | -- | -- | -- | -- | -- | 54 | -- | -- | None |
1 - 10 - 8 - 14 - 82
1 - 0 - 0 - 0 - 0 115 off-targets |
show all... |
| 106 / rev |
TGGTGAGCTGCGAGAATAGC CGG
Enzymes: MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI
Cloning / PCR primers
|
78 | 43 | -- | -- | -- | -- | -- | 54 | -- | -- | None |
0 - 0 - 1 - 9 - 109
0 - 0 - 0 - 0 - 1 119 off-targets |
show all... |
| 232 / fw |
GCCGTCTTCCCCTCCATCGT GGG
Enzymes: BccI
Cloning / PCR primers
|
18 | 51 | -- | -- | -- | -- | -- | 35 | -- | -- | None |
1 - 4 - 17 - 17 - 87
1 - 2 - 0 - 0 - 0 126 off-targets |
|